Cellular biology (cytology) Books
Pan Stanford Publishing Pte Ltd NanoCellBiology: Multimodal Imaging in Biology
Book SynopsisThis book provides a comprehensive understanding of the discovery of a new cellular structure the "porosome," which is the universal secretory machinery in cells; the protein assembly, biomineralization, and biomolecular interactions; the molecular evolution of protein structure; the use of magnetic nanoparticles for transformative application in medicine and therapy, and the new and novel imaging approach of electrical impedance spectroscopy in biology. It be used for college courses in nanomedicine, nano cell biology, advanced nanotechnology, and biotechnology at the undergraduate and graduate level.Trade Review"NanoCellBiology: Multimodal Imaging in Biology and Medicine, edited by Bhanu P. Jena and Douglas J. Taatjes, is a collection of chapters that describe examples of the use of AFM, electron microscopy, photon correlation spectroscopy, confocal microscopy, fluorescence/CD spectroscopy, and other imaging approaches for revealing important structures and their function in cells. A wonderful example is the subject of the first several chapters, which describe the discovery of the porosome. Discovered in the 1990s, first in pancreatic acinar cells, the porosome is now considered a universal secretory portal in cells. The remaining chapters add to this excellent collection of studies employing high-resolution imaging to examine, for example, amylin aggregation, mRNA nanomachines, DNA delivery nanosystems, and other interesting applications of nanocellbiology."Prof. James A. Spudich, Stanford University School of Medicine, USA"Bhanu P. Jena is a pioneer nanocell biologist, whose seminal discovery of a new cell structure called the ‘porosome,’ has provided a molecular understanding of the fractional release of intravesicular contents from cells during secretion. In this book, co-edited by Jena and Douglas J. Taatjes, experts in the field present examples of powerful imaging modalities that have been extremely valuable in elucidating a wide range of normal cellular events, as well as in studying disease progression, detection, and treatment. Chapters in the book provide a lucid explanation of the subject matter, with ample illustrations presented for clarity. This is a timely book, filled with useful resources—a must-read for both researchers and students in cell biology, physiology, biophysics, nanobiology, and nanomedicine."Prof. Walter F. Boron, Case Western Reserve University, USA"NanoCellBiology: Multimodal Imaging in Biology and Medicine is a delightful book co-edited by a pioneer in the subject, Bhanu P. Jena, whose discovery of a new cellular structure, the porosome, in 1996 resulted in a paradigm shift in our understanding of cell secretion and revolutionized our understanding of the unit of life—the cell. This book, edited by Bhanu P. Jena and Douglas J. Taatjes, introduces to the reader a number of exciting subjects within the field of nanoscience and nanomedicine, and the various tools and approaches to solve them for the benefit of science and humanity. I thoroughly enjoyed reading the book and highly recommend to both students in the pure sciences and medicine."Prof. Lloyd L. Anderson, Iowa State University, USATable of ContentsPorosomes -The Universal Secretory Portals In Cells: A Brief Essay. The Hair Cell Porosome: Molecular and Synaptic Implications. The Neuronal Porosome Complex in Mammalian Brain: A Study Using Electron Microscopy. Granule Size Distribution Suggests Mechanism: The Case for Granule Growth and Elimination as a Fusion Nano-Machine. Probing Protein Assembly, Biomineralization, and Biomolecular Interactions by Atomic Force Microscopy. High-Resolution Imaging of Amylin Aggregation and Internalization in Pancreatic Cells: Implications in Health and Diseaser. Repair of Nanodefects in a 2-Dimensional Crystal Anticoagulant Shield in the Antiphospholipid Syndrome: Novel Molecular Strategies Assessed by Atomic Force Microscopy. A novel approach to study molecular evolution: Detection of ancestral conformation hidden in present-day proteins using antibody as nanostructure probes. mRNA Nanomachines and Stress Reprogramming Following Brain Ischemia. Physical Properties and Biomedical Applications of Superparamagnetic Iron Oxide Nanoparticles. Atomic Force Microscopy Imaging of DNA Delivery Nano-systems. Impedance Spectroscopy for Characterization of Biological Functions. Index.
£109.25
Pan Stanford Publishing Pte Ltd Handbook of H+-ATPases
Book SynopsisThis handbook on vacuolar and plasma membrane H+-ATPases is the first to focus on an essential link between vacuolar H+-ATPase and the glycolysis metabolic pathway to understand the mechanism of diabetes and the metabolism of cancer cells. It presents recent findings on the structure and function of vacuolar H+-ATPase in glucose promoting assembly and signaling, in addition to describing the regulatory mechanisms of vacuolar H+-ATPase in yeast cells, neural stem cells, kidney cells, cancer cells, as well as under diabetic conditions. Table of ContentsVacuolar H+-ATPase Assembly. Structure of Prokaryotic V type ATPase/synthase. The function of V-ATPase in the degradation of gluconeogenic enzymes in the yeast vacuole. The Role of Vacuolar ATPase in the Regulation of Npt2a Trafficking. Cytosolic pH regulated by glucose promotes V-ATPase assembly. Vacuolar H+-ATPase (V-ATPase) activated by glucose, a possible link to diabetic disease. Vacuolar proton pump (V-ATPase) and insulin secretion. Role of V-ATPase, cytohesin-2/Arf6 and aldolase in regulation of endocytosis: Implications for diabetic nephropathy. Renal Vacuolar H+-ATPase Regulation. Long-term Regulation of Vacuolar H+-ATPase by Angiotensin II in Proximal Tubule Cells. Vacuolar H+-ATPase in Distal Renal Tubular Acidosis and Diabetes. Vacuolar H+-ATPase in Cancer and Diabetes. The a2 isoform of Vacuolar ATPase and Cancer-Related Inflammation. V-ATPases in oral squamous cell carcinoma. Vacuolar H(+)-ATPase : functional mechanism and potential as a target for cancer chemotherapy. Vacuolar H+-ATPase Maintains Neural Stem Cells in the Developing Mouse Cortex. The relationship between glucose-induced calcium signaling and activation of the plasma membrane H+-ATPase in Saccharomyces cerevisiae cells.
£147.25
Pan Stanford Publishing Pte Ltd Cancer Genetics and Genomics for Personalized
Book SynopsisThis book covers almost all fields of cancer genetics and genomics for personalized medicine. Targeted therapy, or precision medicine, or personalized medicine is becoming a standard treatment for many diseases, including cancer. However, how much do we know about the personalized medicine approach? This lucid book helps undergraduate and graduate students, professional researchers, and clinicians to better understand the key concept of personalized medicine.The most up-to-date topics on personalized medicine in this book cover the recent trends in and updates on lung, gastric, liver, breast, and other types of cancers. Circulating tumor cell, cell-free circulating DNA, and microRNAs are discussed as new diagnostic and prognostic markers for cancer. The avatar mouse model is also discussed for maximizing treatment efficacy and prognosis prediction, and so is microenvironment as a drug resistance mechanism. With classical and new pathological approaches, the book provides a systemic overview of personalized immunotherapies and hyperthermic intraperitoneal chemotherapy, followed by new emerging fields of hereditary cancer, thereby equipping readers to eventually contribute in developing more advanced tools and therapies for curing cancer.Trade Review"Likely as the recent Cancer Moonshot Initiative highlights, this book well describes the importance of genetics/genomics-based personalized therapies to improve cancer treatments. This field is growing rapidly, and it is certain that many scientists should obtain professional knowledge of ‘cancer precision medicine’ to cure more cancer patients."—Prof. Yusuke Nakamura, University of Chicago, USA"At a time when an explosive increase in data has combined with rapidly evolving treatment paradigms, it has become particularly challenging for modern clinicians and medical researchers alike to put these advances in knowledge and practice into a proper context. It is in light of this predicament that this new volume edited by Dr. Il-Jin Kim provides a much-needed organization and harmonic understanding to the cacophony of information regarding the elusive realm of precision medicine. Covering an exhaustive array of topics ranging from the basic science of tumor modeling to the biology of tumor microenvironments to the horizon of cancer immunotherapy and advances for specific cancer types, this clearly written and very readable compilation provides a solid foundation for comprehending the rapidly advancing dawn of a truly new, personalized, precision approach to the treatment and, hopefully, the cure of cancer."—Prof. Michael J. Mann, University of California, San FranciscoTable of ContentsPersonalized Medicine for Cancer: Introduction and Overview of the Book. Personalized Medicine in Lung Cancer. Genome-Based Personalized Medicine in Liver Cancer. Applications of Circulating DNA Analysis in Personalized Medicine. Circulating Tumor Cells and Personalized Medicine. Mouse Models in Personalized Cancer Medicine. Tumor Microenvironment, Therapeutic Resistance, and Personalized Medicine. Personalized Immune Therapy. Hyperthermic Intraperitoneal Chemotherapy (HIPEC) for Peritoneal Malignancies. Personalized Medicine in Hereditary Cancer Syndromes. Pathology in the Era of Personalized Medicine. MicroRNAs in Human Cancers. Pharmacogenomics of Tamoxifen.
£102.60
The University of Chicago Press In Search of Cell History
Book SynopsisThe origin of cells remains one of the most fundamental problems in biology, one that over the past two decades has spawned a large body of research and debate. This book offers a comprehensive, impartial take on that research and the controversies that keep the field in turmoil.Trade Review"This book is a rare pleasure: a beautiful, rational, wise, and eloquent framing of life's greatest mysteries, what remains to be known, and how we might get there. It should be read by anyone who wonders, seriously, how we came to be. If it does not provide all the answers, that is because we honestly do not know." (Nick Lane, University College London)"
£96.90
The University of Chicago Press The Cell
Book Synopsis
£36.55
The University of Chicago Press The Third Lens
Book SynopsisAn analysis of the role of metaphor in shaping the work and findings of science, using cell biology as the central case study.
£76.00
The University of Chicago Press The Third Lens
Book SynopsisAn analysis of the role of metaphor in shaping the work and findings of science, using cell biology as the central case study.
£26.00
The University of Chicago Press Plant Allometry The Scaling of Form and Process
Book SynopsisAllometry, the study of the growth rate of an organism's parts in relation to the whole, has produced various results in research on animals. This text applies allometry to studies of the evolution, morphology, physiology and reproduction of plants.
£42.75
The University of Chicago Press The Evolutionary Origins of Life and Death
Book SynopsisTrade Review"That life is connected to death, and to evolution, is incontrovertible. But what might be the connection between death and evolution? That is, might death be an adaptation? That’s the startling conclusion reached by Durand in this brief volume, which combines philosophy, biological theory, and empirical science in often thrilling ways... Durand thinks big thoughts but is careful, in his prose, to involve readers whose interest in theoretical biology exceeds their grasp. A very important book. Highly recommended." * Choice Connect *"[The Evolutionary Origins of Life and Death] constitutes an impressive effort to integrate into a unified and coherent framework different and dispersed pieces of empirical work about how life and death emerged on Earth, and about how these two phenomena coevolved to become deeply entangled." * History and Philosophy of Life Sciences *“An eye opener for many readers, biologists, and others interested in visiting the frontiers of today’s conceptual thinking on evolution. This book, compact and simple but densely packed with information and ideas, presents the new edifice of evolutionary biology, a vibrant, progressing field that goes far beyond the confines of neo-Darwinism.” -- Eugene V. Koonin, National Center for Biotechnology Information, from the foreword“There is no question that Durand is deeply learned. The way that he lays out his argument linking life and death is certainly innovative and I am sure will attract attention. Insightful and stimulating, The Evolutionary Origins of Life and Death will forward discussion on important issues.” -- Michael Ruse, Florida State UniversityTable of ContentsForeword (by Eugene V. Koonin) Preface List of abbreviations An introduction to the scientific study of life and deathPart One: The origin of life 1 Philosophical considerations and the origin of life 2 The biotic world 3 The theory of life’s origins 4 Life at the very beginning I: the chemistry of the first biomolecules 5 Life at the very beginning II: the emergence of complex RNA molecules 6 The origin of life was an evolutionary transition in individuality 7 A synthesis for the origin of lifePart Two: The origin of death 8 Philosophical considerations and the origin of death 9 Observations of death 10 Mechanisms and measures of programmed cell death in the unicellular world 11 True PCD: when PCD is an adaptation 12 Ersatz PCD: the non-adaptive explanations for PCD 13 Programmed cell death and the levels of selection 14 A synthesis for the origin of programmed cell deathPart Three: Origins of life and death, and their coevolution 15 Group selection and the origins of life and death 16 Life and death coevolution, and the emergence of complexity Postface Additional notes Reference list Index
£87.40
The University of Chicago Press The Evolutionary Origins of Life and Death
Book SynopsisTrade Review"That life is connected to death, and to evolution, is incontrovertible. But what might be the connection between death and evolution? That is, might death be an adaptation? That’s the startling conclusion reached by Durand in this brief volume, which combines philosophy, biological theory, and empirical science in often thrilling ways... Durand thinks big thoughts but is careful, in his prose, to involve readers whose interest in theoretical biology exceeds their grasp. A very important book. Highly recommended." * Choice Connect *"[The Evolutionary Origins of Life and Death] constitutes an impressive effort to integrate into a unified and coherent framework different and dispersed pieces of empirical work about how life and death emerged on Earth, and about how these two phenomena coevolved to become deeply entangled." * History and Philosophy of Life Sciences *“An eye opener for many readers, biologists, and others interested in visiting the frontiers of today’s conceptual thinking on evolution. This book, compact and simple but densely packed with information and ideas, presents the new edifice of evolutionary biology, a vibrant, progressing field that goes far beyond the confines of neo-Darwinism.” -- Eugene V. Koonin, National Center for Biotechnology Information, from the foreword“There is no question that Durand is deeply learned. The way that he lays out his argument linking life and death is certainly innovative and I am sure will attract attention. Insightful and stimulating, The Evolutionary Origins of Life and Death will forward discussion on important issues.” -- Michael Ruse, Florida State UniversityTable of ContentsForeword (by Eugene V. Koonin) Preface List of abbreviations An introduction to the scientific study of life and deathPart One: The origin of life 1 Philosophical considerations and the origin of life 2 The biotic world 3 The theory of life’s origins 4 Life at the very beginning I: the chemistry of the first biomolecules 5 Life at the very beginning II: the emergence of complex RNA molecules 6 The origin of life was an evolutionary transition in individuality 7 A synthesis for the origin of lifePart Two: The origin of death 8 Philosophical considerations and the origin of death 9 Observations of death 10 Mechanisms and measures of programmed cell death in the unicellular world 11 True PCD: when PCD is an adaptation 12 Ersatz PCD: the non-adaptive explanations for PCD 13 Programmed cell death and the levels of selection 14 A synthesis for the origin of programmed cell deathPart Three: Origins of life and death, and their coevolution 15 Group selection and the origins of life and death 16 Life and death coevolution, and the emergence of complexity Postface Additional notes Reference list Index
£31.00
University of Notre Dame Press Stem Cell Research
Book SynopsisThis volume brings together essays by an internationally distinguished and diverse group of scholars. Contributors thoughtfully explore the ethical, public policy, and scientific implications of embryonic and adult stem cell research. Part one of the book offers a variety of scientific and public policy perspectives, including essays on stem cell plasticity and using umbilical cord blood as an alternative source of pluripotent stem cells. Part two vigorously examines the ethics of stem cell research and considers issues of social justice, morality, and public policy. Scientific alternatives, a natural law perspective regarding federal funding, and a discussion of the possible moral complicity of Catholic researchers are among the distinctive contributions made to the stem cell research debate by this collection. The objective and balanced discussions contained in this volume serve as an accessible introduction to the bioethical questions, issues, and problems surroundiTrade Review“This collection covers a broad range of scientific, ethical, and public policy issues. Some articles provide general background and information, while others make highly original contributions to the ongoing debate. Ethical issues are discussed knowledgeably from a variety of religious and philosophical perspectives. Thoughtful disagreements among authors illustrate the difficulty of reaching a consensus on public policy.” —Carol A. Tauer, Professor of Philosophy Emerita, The College of St. Catherine, St. Paul, MN"In the book, Stem Cell Research, Nancy Snow provides a collection of essays that discuss different aspects of the stem cell debate. This is an important endeavor. . ." —Dialogue". . . this collection of essays is sure to speak to readers on either side of this divisive issue." —NationalJournal.com"[Stem Cell Research] provides a useful primer for many. . . of the ethical issues presented by the embryonic stem cell debate." —The Quarterly Review of Biology“This collection is an important resource for any bioethics course.” —Theological Studies“. . . so volatile and dynamic are the variables in the debates over this type of research, it would be a mistake not to look upon it as having lasting value. The authors are attentive to cutting edge science to be sure, but they effectively raise up time-tested principles from biomedical ethics and the natural law tradition in an effort to weigh the pros and cons.” —Catholic Library World“Snow’s volume is earnest . . . a plea for ethically informed science.” —Times Literary Supplement“. . . the contributions are thought-provoking and the arguments need to be addressed by proponents of the research.” —Conscience“This volume is a collection of ten essays developed out of a 2001 conference . . . the gathering convened scientists and ethicists to discuss scientific, public policy, and ethical issues concerning human stem cell research . . . the essays offer a wide range of topics, analyses, and arguments, and, as a whole, exhibit some of the complexity of issues involved in discussing human stem cell research-human embryonic stem cell research in particular. As such, the volume is an excellent introduction to the nuanced contours of this contentious realm.” —Pro Ecclesia
£22.79
Springer-Verlag New York Inc. An Atlas of Histology
Book SynopsisBridging the gap between textbook diagrams and the complex reality of histological preparations, this magnificent atlas of human microanatomy is designed to help students understand the complex structures encountered when viewing microscopic sections of tissues. Instead of simply depicting an individual section, each drawing is a compilation of the key structures and features seen in many preparations from similar tissues or organs. Invaluable to students in a range of life science and medical disciplines including human and veterinary medicine, dentistry, mammalian biology, pharmacy, and nursing.Table of Contents1. Epithelial Tissue.- 2. Connective Tissue.- 3. Cartilage and Bone.- 4. Blood Cells and Hemopoietic Cells.- 5. Muscular Tissue.- 6. Nervous Tissue and Nervous System.- 7. Circulatory System.- 8. Lymphatic Organs.- 9. Respiratory System.- 10. Digestive System.- 11. Urinary System.- 12. Male Reproductive System.- 13. Female Reproductive System.- 14. Endocrine Organs.- 15. The Integument.- 16. The Eye.- 17. The Ear.- References.
£123.49
John Wiley & Sons Inc Biophysical Chemistry of Biointerfaces
Book SynopsisBiointerfaces are central to biology and medicine and crucial in research relating to implants, biosensors, drug delivery, proteomics, and many other fields.Trade Review"Ohshima (pharmaceutical science, Tokyo U. of Science) sets out a set of tools for discussing various phenomena at biological interfaces - such as cell surfaces - in terms of biophysical chemistry." (SciTech Book News, December 2010) Table of ContentsPreface xiii List of Symbols xv Part I Potential and Charge at Interfaces 1 1 Potential and Charge of a Hard Particle 3 1.1 Introduction 3 1.2 The Poisson-Boltzmann Equation 3 1.3 Plate 6 1.3.1 Low Potential 8 1.3.2 Arbitrary Potential: Symmetrical Electrolyte 8 1.3.3 Arbitrary Potential: Asymmetrical Electrolyte 13 1.3.4 Arbitrary Potential: General Electrolyte 14 1.4 Sphere 16 1.4.1 Low Potential 17 1.4.2 Surface Charge Density-Surface Potential Relationship: Symmetrical Electrolyte 18 1.4.3 Surface Charge Density-Surface Potential Relationship: Asymmetrical Electrolyte 21 1.4.4 Surface Charge Density-Surface Potential Relationship: General Electrolyte 22 1.4.5 Potential Distribution Around a Sphere with Arbitrary Potential 25 1.5 Cylinder 31 1.5.1 Low Potential 32 1.5.2 Arbitrary Potential: Symmetrical Electrolyte 33 1.5.3 Arbitrary Potential: General Electrolytes 34 1.6 Asymptotic Behavior of Potential and Effective Surface Potential 37 1.6.1 Plate 38 1.6.2 Sphere 41 1.6.3 Cylinder 42 1.7 Nearly Spherical Particle 43 References 45 2 Potential Distribution Around a Nonuniformly Charged Surface and Discrete Charge Effects 47 2.1 Introduction 47 2.2 The Poisson-Boltzmann Equation for a Surface with an Arbitrary Fixed Surface Charge Distribution 47 2.3 Discrete Charge Effect 56 References 62 3 Modified Poisson-Boltzmann Equation 63 3.1 Introduction 63 3.2 Electrolyte Solution Containing Rod-like Divalent Cations 63 3.3 Electrolyte Solution Containing Rod-like Zwitterions 70 3.4 Self-atmosphere Potential of Ions 77 References 82 4 Potential and Charge of a Soft Particle 83 4.1 Introduction 83 4.2 Planar Soft Surface 83 4.2.1 Poisson–Boltzmann Equation 83 4.2.2 Potential Distribution Across a Surface Charge Layer 87 4.2.3 Thick Surface Charge Layer and Donnan Potential 90 4.2.4 Transition Between Donnan Potential and Surface Potential 91 4.2.5 Donnan Potential in a General Electrolyte 92 4.3 Spherical Soft Particle 93 4.3.1 Low Charge Density Case 93 4.3.2 Surface Potential–Donnan Potential Relationship 95 4.4 Cylindrical Soft Particle 100 4.4.1 Low Charge Density Case 100 4.4.2 Surface Potential–Donnan Potential Relationship 101 4.5 Asymptotic Behavior of Potential and Effective Surface Potential of a Soft Particle 102 4.5.1 Plate 102 4.5.2 Sphere 103 4.5.3 Cylinder 104 4.6 Nonuniformly Charged Surface Layer: Isoelectric Point 104 References 110 5 Free Energy of a Charged Surface 111 5.1 Introduction 111 5.2 Helmholtz Free Energy and Tension of a Hard Surface 111 5.2.1 Charged Surface with Ion Adsorption 111 5.2.2 Charged Surface with Dissociable Groups 116 5.3 Calculation of the Free Energy of the Electrical Double Layer 118 5.3.1 Plate 119 5.3.2 Sphere 120 5.3.3 Cylinder 121 5.4 Alternative Expression for Fel 122 5.5 Free Energy of a Soft Surface 123 5.5.1 General Expression 123 5.5.2 Expressions for the Double-Layer Free Energy for a Planar Soft Surface 127 5.5.3 Soft Surface with Dissociable Groups 128 References 130 6 Potential Distribution Around a Charged Particle in a Salt-Free Medium 132 6.1 Introduction 132 6.2 Spherical Particle 133 6.3 Cylindrical Particle 143 6.4 Effects of a Small Amount of Added Salts 146 6.5 Spherical Soft Particle 152 References 162 Part II Interaction Between Surfaces 163 7 Electrostatic Interaction of Point Charges in an Inhomogeneous Medium 165 7.1 Introduction 165 7.2 Planar Geometry 166 7.3 Cylindrical Geometry 180 References 185 8 Force and Potential Energy of the Double-Layer Interaction Between Two Charged Colloidal Particles 186 8.1 Introduction 186 8.2 Osmotic Pressure and Maxwell Stress 186 8.3 Direct Calculation of Interaction Force 188 8.4 Free Energy of Double-Layer Interaction 198 8.4.1 Interaction at Constant Surface Charge Density 199 8.4.2 Interaction at Constants Surface Potential 200 8.5 Alternative Expression for the Electric Part of the Free Energy of Double-Layer Interaction 201 8.6 Charge Regulation Model 201 References 202 9 Double-Layer Interaction Between Two Parallel Similar Plates 203 9.1 Introduction 203 9.2 Interaction Between Two Parallel Similar Plates 203 9.3 Low Potential Case 207 9.3.1 Interaction at Constant Surface Charge Density 208 9.3.2 Interaction at Constant Surface Potential 211 9.4 Arbitrary Potential Case 214 9.4.1 Interaction at Constant Surface Charge Density 214 9.4.2 Interaction at Constant Surface Potential 224 9.5 Comparison Between the Theory of Derjaguin and Landau and the Theory of Verwey and Overbeek 226 9.6 Approximate Analytic Expressions for Moderate Potentials 227 9.7 Alternative Method of Linearization of the Poisson–Boltzmann Equation 231 9.7.1 Interaction at Constant Surface Potential 231 9.7.2 Interaction at Constant Surface Charge Density 234 References 240 10 Electrostatic Interaction Between Two Parallel Dissimilar Plates 241 10.1 Introduction 241 10.2 Interaction Between Two Parallel Dissimilar Plates 241 10.3 Low Potential Case 244 10.3.1 Interaction at Constant Surface Charge Density 244 10.3.2 Interaction at Constant Surface Potential 251 10.3.3 Mixed Case 252 10.4 Arbitrary Potential: Interaction at Constant Surface Charge Density 252 10.4.1 Isodynamic Curves 252 10.4.2 Interaction Energy 258 10.5 Approximate Analytic Expressions for Moderate Potentials 262 References 263 11 Linear Superposition Approximation for the Double-Layer Interaction of Particles at Large Separations 265 11.1 Introduction 265 11.2 Two Parallel Plates 265 11.2.1 Similar Plates 265 11.2.2 Dissimilar Plates 270 11.2.3 Hypothetical Charge 276 11.3 Two Spheres 278 11.4 Two Cylinders 279 References 281 12 Derjaguin’s Approximation at Small Separations 283 12.1 Introduction 283 12.2 Two Spheres 283 12.2.1 Low Potentials 285 12.2.2 Moderate Potentials 286 12.2.3 Arbitrary Potentials: Derjaguin’s Approximation Combined with the Linear Superposition Approximation 288 12.2.4 Curvature Correction to Derjaguin’ Approximation 290 12.3 Two Parallel Cylinders 292 12.4 Two Crossed Cylinders 294 References 297 13 Donnan Potential-Regulated Interaction Between Porous Particles 298 13.1 Introduction 298 13.2 Two Parallel Semi-infinite Ion-penetrable Membranes (Porous Plates) 298 13.3 Two Porous Spheres 306 13.4 Two Parallel Porous Cylinders 310 13.5 Two Parallel Membranes with Arbitrary Potentials 311 13.5.1 Interaction Force and Isodynamic Curves 311 13.5.2 Interaction Energy 317 13.6 pH Dependence of Electrostatic Interaction Between Ion-penetrable Membranes 320 References 322 14 Series Expansion Representations for the Double-Layer Interaction Between Two Particles 323 14.1 Introduction 323 14.2 Schwartz’s Method 323 14.3 Two Spheres 327 14.4 Plate and Sphere 342 14.5 Two Parallel Cylinders 348 14.6 Plate and Cylinder 353 References 356 15 Electrostatic Interaction Between Soft Particles 357 15.1 Introduction 357 15.2 Interaction Between Two Parallel Dissimilar Soft Plates 357 15.3 Interaction Between Two Dissimilar Soft Spheres 363 15.4 Interaction Between Two Dissimilar Soft Cylinders 369 References 374 16 Electrostatic Interaction Between Nonuniformly Charged Membranes 375 16.1 Introduction 375 16.2 Basic Equations 375 16.3 Interaction Force 376 16.4 Isoelectric Points with Respect To Electrolyte Concentration 378 Reference 380 17 Electrostatic Repulsion Between Two Parallel Soft Plates After Their Contact 381 17.1 Introduction 381 17.2 Repulsion Between Intact Brushes 381 17.3 Repulsion Between Compressed Brushes 382 References 387 18 Electrostatic Interaction Between Ion-Penetrable Membranes In a Salt-free Medium 388 18.1 Introduction 388 18.2 Two Parallel Hard Plates 388 18.3 Two Parallel Ion-Penetrable Membranes 391 References 398 19 van der Waals Interaction Between Two Particles 399 19.1 Introduction 399 19.2 Two Molecules 399 19.3 A Molecule and a Plate 401 19.4 Two Parallel Plates 402 19.5 A Molecule and a Sphere 404 19.6 Two Spheres 405 19.7 A Molecule and a Rod 407 19.8 Two Parallel Rods 408 19.9 A Molecule and a Cylinder 408 19.10 Two Parallel Cylinders 410 19.11 Two Crossed Cylinders 412 19.12 Two Parallel Rings 412 19.13 Two Parallel Torus-Shaped Particles 413 19.14 Two Particles Immersed In a Medium 417 19.15 Two Parallel Plates Covered with Surface Layers 418 References 419 20 DLVO Theory of Colloid Stability 420 20.1 Introduction 420 20.2 Interaction Between Lipid Bilayers 420 20.3 Interaction Between Soft Spheres 425 References 429 Part III Electrokinetic Phenomena at Interfaces 431 21 Electrophoretic Mobility of Soft Particles 433 21.1 Introduction 433 21.2 Brief Summary of Electrophoresis of Hard Particles 433 21.3 General Theory of Electrophoretic Mobility of Soft Particles 435 21.4 Analytic Approximations for the Electrophoretic Mobility of Spherical Soft Particles 440 21.4.1 Large Spherical Soft Particles 440 21.4.2 Weakly Charged Spherical Soft Particles 444 21.4.3 Cylindrical Soft Particles 447 21.5 Electrokinetic Flow Between Two Parallel Soft Plates 449 21.6 Soft Particle Analysis of the Electrophoretic Mobility of Biological Cells and Their Model Particles 454 21.6.1 RAW117 Lymphosarcoma Cells and Their Variant Cells 454 21.6.2 Poly(N-isopropylacrylamide) Hydrogel-Coated Latex 455 21.7 Electrophoresis of Nonuniformly Charged Soft Particles 457 21.8 Other Topics of Electrophoresis of Soft Particles 463 References 464 22 Electrophoretic Mobility of Concentrated Soft Particles 468 22.1 Introduction 468 22.2 Electrophoretic Mobility of Concentrated Soft Particles 468 22.3 Electroosmotic Velocity in an Array of Soft Cylinders 475 References 479 23 Electrical Conductivity of a Suspension of Soft Particles 480 23.1 Introduction 480 23.2 Basic Equations 480 23.3 Electrical Conductivity 481 References 484 24 Sedimentation Potential and Velocity in a Suspension of Soft Particles 485 24.1 Introduction 485 24.2 Basic Equations 485 24.3 Sedimentation Velocity of a Soft Particle 490 24.4 Average Electric Current and Potential 490 24.5 Sedimentation Potential 491 24.6 Onsager’s Reciprocal Relation 494 24.7 Diffusion Coefficient of a Soft Particle 495 References 495 25 Dynamic Electrophoretic Mobility of a Soft Particle 497 25.1 Introduction 497 25.2 Basic Equations 497 25.3 Linearized Equations 499 25.4 Equation of Motion of a Soft Particle 501 25.5 General Mobility Expression 501 25.6 Approximate Mobility Formula 503 References 506 26 Colloid Vibration Potential in a Suspension of Soft Particles 508 26.1 Introduction 508 26.2 Colloid Vibration Potential and Ion Vibration Potential 508 References 513 27 Effective Viscosity of a Suspension of Soft Particles 515 27.1 Introduction 515 27.2 Basic Equations 516 27.3 Linearized Equations 518 27.4 Electroviscous Coefficient 520 27.5 Approximation for Low Fixed-Charge Densities 523 27.6 Effective Viscosity of a Concentrated Suspension of Uncharged Porous Spheres 527 Appendix 27a 530 References 531 Part IV other Topics 533 28 Membrane Potential and Donnan Potential 535 28.1 Introduction 535 28.2 Membrane Potential and Donnan Potential 535 References 541 Index 543
£127.76
John Wiley & Sons Inc Plant Cell Culture Essential Methods
Book SynopsisThe ability to culture cells is fundamental for mass propagationand as a baseline for the genetic manipulation of plant nuclei andorganelles.Trade Review"In summary, this book is important for plant scientists as it thoroughly covers elementary technologies, providing the tools for comprehensive plant research." (The Quarterly Review of Biology, 1 September 2011) "For anyone working in the area of plant tissue culture, micro-propagation or transformation this book is essential reading." (The Journal of Experimental Agriculture, 1 October 2011)Table of ContentsPreface. Contributors. 1 Plant Micropropagation (Ivan Iliev, Alena Gajdosova, Gabriela Libiakova, Shri Mohan Jain). 1.1 Introduction. 1.2 Methods and approaches. 1.2.1 Explants and their surface disinfection. 1.2.2 Culture media and their preparation. 1.2.3 Stages of micropropagation. 1.2.4 Techniques of micropropagation. 1.3 Troubleshooting. References. 2 Thin Cell Layers: The Technique (Jaime A. Teixeira da Silva and Michio Tanaka). 2.1 Introduction. 2.2 Methods and approaches. 2.2.1 TCL. 2.2.2 Choice of material: Cymbidium hybrid. 2.3 Troubleshooting. 2.3.1 General comments. References. 3 Plant Regeneration – Somatic Embryogenesis (Kim E. Nolan, Ray J. Rose). 3.1 Introduction. 3.2 Methods and approaches. 3.2.1 Selection of the cultivar and type of explant. 3.2.2 Culture media. 3.2.3 Preparation of culture media. 3.2.4 Sterilization of tissues and sterile technique. 3.2.5 Culture and growth of tissue. 3.2.6 Culture and induction of somatic embryos. 3.2.7 Embryo development. 3.2.8 Transfer to soil – the final stage of regeneration. 3.3 Troubleshooting. References. 4 Haploid Plants (Sant S. Bhojwani and Prem K. Dantu). 4.1 Introduction. 4.2 Methods and approaches. 4.2.1 Androgenesis. 4.2.2 Diploidization. 4.3 Troubleshooting. References. 5 Embryo Rescue (Traud Winkelmann, Antje Doil, Sandra Reinhardt and Aloma Ewald). 5.1 Introduction. 5.2 Methods and approaches. 5.2.1 Identification of the time and type of barrier in hybridization. 5.2.2 Isolation of plant material after fertilization. 5.2.3 Culture conditions and media. 5.2.4 Confirmation of hybridity and ploidy. 5.2.5 Conditions for regeneration of embryos to plants. 5.3 Troubleshooting. References. 6 In vitro Flowering and Seed Set: Acceleration of Generation Cycles (Sergio J. Ochatt and Rajbir S. Sangwan). 6.1 Introduction. 6.2 Methods and approaches. 6.2.1 Protein legumes [7]. 6.2.2 Arabidopsis thaliana [13]. 6.3 Troubleshooting. References. 7 Induced Mutagenesis in Plants Using Physical and Chemical Agents (Chikelu Mba, Rownak Afza, Souleymane Bado and Shri Mohan Jain). 7.1 Introduction. 7.2 Methods and approaches. 7.2.1 Determination of the optimal doses of mutagens for inducing mutations. 7.3 Troubleshooting. 7.3.1 Factors influencing the outcome of mutagenesis using chemical mutagens. 7.3.2 Factors influencing the outcome of mutagenesis using physical mutagens. 7.3.3 Facts about induced mutations. References. 8 Cryopreservation of Plant Germplasm (E.R. Joachim Keller and Angelika Senula). 8.1 Introduction. 8.2 Methods and approaches. 8.2.1 Main principles. 8.2.2 Slow (two-step) freezing. 8.2.3 Vitrification. 8.2.4 Encapsulation–dehydration. 8.2.5 DMSO droplet freezing. 8.2.6 Combined methods. 8.2.7 Freezing of cold-hardened buds. 8.2.8 Freezing of orthodox seeds. 8.2.9 Freezing of pollen and spores. 8.3 Troubleshooting. References. 9 Plant Protoplasts: Isolation, Culture and Plant Regeneration (Michael R. Davey, Paul Anthony, Deval Patel and J. Brian Power). 9.1 Introduction. 9.2 Methods and approaches. 9.2.1 Protoplast isolation. 9.2.2 Protoplast culture. 9.3 Troubleshooting. References. 10 Protoplast Fusion Technology – Somatic Hybridization and Cybridization (Jude W. Grosser, Milica Calovic and Eliezer S. Louzada). 10.1 Introduction. 10.2 General applications of somatic hybridization. 10.3 Methods and approaches. 10.4 Troubleshooting. References. 11 Genetic Transformation – Agrobacterium (Ian S. Curtis). 11.1 Introduction. 11.2 Methods and approaches. 11.2.1 Agrobacterium as a natural genetic engineer. 11.2.2 Vector systems for transformation. 11.2.3 Inoculation procedures. 11.3 Troubleshooting. References. 12 Genetic Transformation – Biolistics (Fredy Altpeter and Sukhpreet Sandhu). 12.1 Introduction. 12.2 Methods and approaches. 12.2.1 Biolistic technology. 12.2.2 Optimization of gene delivery parameters. 12.2.3 Target tissues. 12.2.4 Reporter gene assays. 12.2.5 Selection and plant regeneration. 12.3 Troubleshooting. References. 13 Plastid Transformation (Bridget V. Hogg, Cilia L.C. Lelivelt, Aisling Dunne, Kim-Hong Nguyen and Jacqueline M. Nugent). 13.1 Introduction. 13.2 Methods and approaches. 13.2.1 Principles of plastid transformation. 13.2.2 Biolistic-mediated plastid transformation. 13.2.3 PEG-mediated plastid transformation. 13.2.4 Identification and characterization of transplastomic plants. 13.3 Troubleshooting. 13.3.1 Biolistic-mediated transformation. 13.3.2 PEG-mediated transformation. References. 14 Molecular Characterization of Genetically Manipulated Plants (Cristiano Lacorte, Giovanni Vianna, Francisco J.L. Aragao and El´ıbio L. Rech). 14.1 Introduction. 14.2 Methods and approaches. 14.2.1 Plant DNA extraction. 14.2.2 Polymerase chain reaction. 14.2.3 Southern blot technique. 14.2.4 Analysis of the integration site: inverse PCR (iPCR) and thermal asymmetric interlaced PCR (Tail-PCR). 14.3 Troubleshooting. References. 15 Bioreactors (Spiridon Kintzios). 15.1 Introduction. 15.2 Methods and approaches. 15.2.1 Medium scale disposable or semidisposable airlift reactors. 15.2.2 The RITA temporary immersion reactor. 15.2.3 The LifeReactor. 15.2.4 Immobilized cell bioreactors. 15.2.5 Mini-bioreactors. 15.3 Troubleshooting. References. 16 Secondary Products (Kexuan Tang, Lei Zhang, Junfeng Chen, Ying Xiao, Wansheng Chen and Xiaofen Sun). 16.1 Introduction. 16.2 Methods and approaches. 16.2.1 Plant cell cultures. 16.2.2 Scale-up and regulation of secondary metabolite production. 16.2.3 Detection of secondary products. 16.3 Troubleshooting. References. 17 Plant Cell Culture – Present and Future (Jim M. Dunwell). 17.1 Introduction. 17.2 Micropropagation. 17.3 Embryogenesis. 17.3.1 Background. 17.3.2 Commercial exploitation of somatic embryos. 17.3.3 Molecular aspects of somatic embryogenesis. 17.3.4 Microspore derived embryos. 17.4 Haploid methodology. 17.4.1 Haploids and their exploitation. 17.4.2 Induction of haploid plants. 17.4.3 Molecular aspects of haploid induction from microspores. 17.4.4 Ab initio zygotic-like embryogenesis from microspores. 17.5 Somaclonal variation. 17.6 Transgenic methods. 17.6.1 Background. 17.6.2 Regeneration and transformation techniques. 17.6.3 Chloroplast transformation. 17.6.4 Biopharming. 17.7 Protoplasts and somatic hybridization. 17.8 Bioreactors. 17.8.1 Production of plant products. 17.8.2 Production of pharmaceuticals. 17.8.3 Production of food ingredients. 17.8.4 Production of cosmetics. 17.8.5 Analytical methodology. 17.9 Cryopreservation. 17.10 Intellectual property and commercialization. 17.10.1 Background. 17.10.2 Sources of patent and other relevant information. 17.11 Conclusion. References. Index.
£77.36
John Wiley & Sons Inc Culture of Hematopoietic Cells
Book SynopsisThis guide provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of haemopoietic cells. Covering a range of human and mouse cell types, and featuring step-by-step laboratory procedures, it gives detailed instructions for reagent preparation.Table of ContentsPartial table of contents: PRIMITIVE PROGENITOR ASSAYS. Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher). Assay for Murine Blast Cell Colonies (N. Katayama & M.Ogawa). Serum-Deprived Cultures of Primary Hematopoietic Cells (G.Migliaccio & A. Migliaccio). COLONY-FORMING ASSAYS FOR MALIGNANT CELLS. Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, etal.). LONG-TERM ASSAYS AND IMMORTALIZATION. Long-Term Culture of Human Myeloid Cells (H. Sutherland & C.Eaves). Immortalization of Hematopoietic Cells (C. MacDonald). PURIFICATION OF PROGENITOR CELLS. Purification of Murine Granulocyte-Macrophage Progenitor Cells(CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper &H. Broxmeyer). OTHER SYSTEMS AND ASSAYS. Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins& R. Fleischman). List of Suppliers. Index.
£171.86
Wiley Medical Biofilms v2 Detection Prevention and
Book SynopsisBiofilms are formed by microorganisms growing on surfaces and comprise a series of microcolonies interspersed with spaces through which fluids and other microorganisms move. This book focuses on medical biofilms, covering information on the problems of biofilms, how to detect them and how to control their presence.Table of ContentsContributors. Preface. Glossary. 1. Microbial Biofilms in Medicine (J, Jass, S. Surman and J. Walker). 2. Biofilms Associated with Medical Devices and Implants. Problems of Biofilms Associated with Medical Devices and Implants (R. Donlan). Pathogenesis and Detection of Biofilm Formation, on Medical Impants (C. Von Eiff and G. Peters). Control of Biofilms Associated With Implanted Medical Devices (P. Gilbert, et al.). 3. Microbial Adhesion and Biofilm Formation on Tissue Surfaces. Biofilm-Related Infections on Tissue Surfaces (S. Wai, Y. Mizunoe and J. Jass). Interaction of Biofilms with Tissues (M. Olson, H. Ceri and D. Morck). Control of Microbial Adhesion and Biofilm Formation on Tissue Surfaces (G. Reid, et al. ). 4. Dental Plaque and Bacterial Colonization of Dental Materials. Dental Plaque and Bacterial Colonization (D. Spratt). Detection of Microorganisms in Dental Plaque (D. Dymock). Control of Dental Plaque (R. Sammons). 5. Biofilms Past, Present and Future-New Methods and Control Strategies in Medicine (J. Walker, S. Surman and J. Jass). Index.
£145.76
John Wiley and Sons Ltd Molecular Methods in Ecology
Book Synopsis* Provides up-to-date summaries of the latest molecular approaches in this rapidly expanding field. * Gives guidance on the appropriate choice of methods for particular problems in ecology, and their strengths and limitations.Table of Contents1. Molecular Ecology (A J Baker). 2. General Molecular Biology (R E Carter). 3. Polymerase Chain Reaction (T P Birt & A J Baker). 4. Protein Electrophoresis (A J Baker). 5. Solution DNA-DNA Hybridisation (A H Bledsoe & F H Sheldon). 6. DNA Fingerprinting using minisatellite probes (R E Carter). 7. Mitochondrial DNA (E Randi). 8. Characterization and Evolution of Major Histocompatibility Complex (Mhc) Geners in Non-Model Organisms, with Examples from Birds (S V Edwards, J Nusser & J Gasper). 9. DNA-fragment markers in plants (C Ritland & K Ritland). 10. Microsatellites: Evolutionary and Methodological Background and Empirical Applications at individual, population and phylogenetic levels (K T Scribner & J M Pearce). 11. Introns (V Friesen). 12. Sex Identification using DNA markers (R Griffiths)
£98.06
John Wiley and Sons Ltd Route Maps in Gene Technology
Book SynopsisGene-based techniques have revolutionized our understanding of biology and had a direct impact on many aspects of our lives, from clinical medicine to agriculture. This book allows the reader to choose from pathways in discovering the concepts of molecular biology, the principles of a recombinant DNA technology and its key methods.Table of ContentsPreface. About the Route Maps format. The Concept Of Genes Is Developed. Genes Are Located To Chromosomes. Genes Are Composed Of DNA. The Chemical Building Blocks Of Nucelic Acids. Formation Of The DNA Double Helix. Packaging Of DNA Within Cells. Chromatin Structure And The Functional Activity Of Genes. Types And Functions Of DNA-Protein Interactions. Organization Of Genomes Into Multiple Chromosomes. Distribution Of Nucleic Acids Within Eukaryotic Cells. Types Of RNA Molecules. The Anatomy Of Eukaryotic Chromosomes. The Organisation Of Genes Within Chromosomes. The Molecular Anatomy Of Eukaryotic Genes. Chromosome Aberrations And Human Disease. Types Of Mutations And Their Effects. Forms Of Chemically Altered DNA; DNA Repair Mechanisms. Linkage Analysis. Pedigree Analysis And Modes Of Inheritance. Genes Dictate The Nature Of Proteins. The Nature Of The Genetic Code. Transcription: Forming Genetic Messages. Post-Transcriptional Processing Of Messenger RNA. Transfer And Ribosomal RNA Processing/Modification. Mechanisms Regulating Gene Expression. Transcriptional Regulatory Sequences. Operons And Prokaryotic Control Of Gene Expression. Transcription Factors And Gene Expression. In Vivo Translation: Decoding Genetic Messages. Sequences Involved In Cellular Protein Targeting. Eukaryotic Cell Division: Mitosis And Meiosis. Molecular Mechanisms Of Cell Cycle Control. Genetic Recombination Mechanisms. Gene Transfer During Bacterial Reproduction. Transposable Genetic Elements: Transposons. In Vivo DNA Replication. Genetic Control Of Development. The Natural Biology Of Bacteriophages. Bacteriophage Genetics. Recombinant DNA Technology. Enzymes Commonly Used In Molecular Biology Methods. Restriction Endonucleases. Restriction Fragment Length Polymorphisms. Isolation Of Nucleic Acids From Cells And Tissues. Visualising Mucleic Acids. Electrophoresis Of Nucleic Acids. In Vitro Hybridisation. Types Of Hybridisation Assay Formats. Southern Blotting; In Situ Hybridisation. Measuring Transcriptional Activity Via Messenger RNA. Converting Messenger RNA Into Complementary DNA (Cdna). Methods For Determining DNA Nucleotide Sequences. The Polymerase Chain Reaction. Alternatives To PCR-Based In Vitro DNA/RNA Amplification. In Vitro Translation Methods. Types And Methods Of Gene Probe Generation. Chemical Synthesis Of Oligonucleotides. Types And Applications Of Nucleotide Analogues. Methods For Labelling Gene Probes. Fundamental Principles Of Cloning. The Nature Of Cloning Vectors. Inserting Foreign DNA Into Vectors. The Development Of Bacteriophage Vectors. Plasmids: Development As Clonign Vectors. Yeast-Derived Plasmid Vectors. Phagemids: Hybrid Phage And Plasmid Vectors. Vectors For Use In Plant And Animal Cells. Delivering DNA Into Cells; Principal Genomic And Cdna Cloning Strategies. Strategies For Identifying Desirable Recombinant Clones. Gene Mapping Techniques. Detecting DNA-Protein Interaction Sites. Detecting Promoter And Enhancer Sequences. Methods For Identifying Protein Encoding Sequences. Genetic Fingerprinting. Analysing Ancient Dnas. In Vitro Mutagenesis Methods. Genetically Modified Micro-Organisms. Genetically Engineered Plants. Genetically Engineered Animals. Molecular Techniques In Prenatal Diagnosis. The Genetics Of Cystic Fibrosis. The Dystrophin Gene And Muscular Dystrophies. Identifying The Gene For Huntingdon's Disease. Lipoprotein Genes And Heart Disease. The Detection Of Microbial Infections. Molecular Biology Of Human Immunodeficiency Virus And AIDS. Engineering Microbial Bioluminescence. Recombinant DNA Techniques In Vaccine Development. Receptors And Cellular Signalling Mechanisms. Oncogenes And The Molecular Basis Of Cancer. Molecular Diagnosis And Therapy Of Cancers. Drug Development Using Recombinant DNA Approaches. Protein Engineering. Immunoglobulin Genetics. Genetic Engineering Of Recombinant Antibodies. Current Approaches To Gene Therapy. The Human Genome Mapping Project. Bibliography. Index.
£84.50
Harvard University Press The Generation of Diversity
Book SynopsisThis book is an intellectual history of the major theoretical problem in immunology and its resolution in the postâWorld War II period. In recent years immunology has been one of the most excitingâand successfulâfields of biomedical research; this book provides essential background for understanding the conceptual conflicts occurring in the field.Trade ReviewScott Podolsky and Alfred Tauber have written a dense and exhaustive scholarly treatise about the history of [the generation of diversity]. They explore the intellectual setting in which the Tonegawa experiment was performed by reviewing original literature and interviewing the major actors in the drama...Everyone interested in the history of immunology and genetics should read this book. -- Fred S. Rosen * Nature *The Generation of Diversity is a remarkable mixture of formal history, literature review, and 'immunophilosophy.' It traces the origins of Burnet's clonal selection theory as a central paradigm of contemporary immunology, then reconciles that theory with the discoveries of the molecular era, and concludes with consideration of 'the immune self'...The authors' meticulously detailed background to the experimental work is a tour de force, which one would never expect to be attempted again, and it stands as a vibrant testament to an extraordinary era for immunology. -- Ian R. Mackay * Science *This book is thoroughly researched, well written, and obviously intended to be definitive with respect to clonal selection and the generation of antibody diversity… Despite, or perhaps because of, the great amount of detail in this book, I enjoyed reading it… For an immunologist, it is satisfying to have the history of two major areas of research recapitulated. This book includes many details I missed earlier or had forgotten, and it is of interest to compare the authors’ assignment of priorities for correct conclusions with those of the principals, as expresses in their own reviews of the topic. This book will be an essential part of the collection of anyone with an interest in the history of immunology. -- Alfred Nisonoff * New England Journal of Medicine *This scholarly historiography is a must for those with more than a passing interest in immunology. Nine well-structured chapters cover the issues that have led to a general acceptance of the clonal selection theory and the establishment of molecular immunology as the arbiter of current thinking… Scott Podolsky and Alfred Tauber’s conceptual grasp of the ideas is anchored by a thorough explanation of critical experiments. They bring an almost extravagant degree of research, and attention to every argument, rendering the finished product as readable as a good novel. -- Richard Lake * Times Higher Education Supplement *Several recent studies have shown the central role of techniques, instruments, reagents and experimental systems in the ‘molecularization of biology and medicine’, first by focusing on the structure of proteins, then on the structure of nucleic acids (DNA and RNA). The Generation of Diversity is a fascinating account of how exactly this change took place in immunology. The book’s main strength [is] telling a very detailed story of a transformation of a single domain of scientific inquiry. -- Ilana Löwy * Medical History [UK] *A balanced and lucid conceptual history of postwar immunology, documenting one of its most fundamental transformations. This is an important and original contribution to the history of twentieth century biology. -- Angela Creager, Princeton UniversityTable of ContentsA conceptual orientation; the Burnet legacy; CST and molecular immunology - a dogmatic alliance; interlude - the conventional history; germline, somatic mutation and recombinatorial models - 1960-1974; immunobiological theories of antibody diversity; from protein to DNA; the recombinant revolution; interlude - a historigraphic reappraisal; heavy chain diversity and the molecular finale; an accounting; the fate of the immune self.
£37.36
Harvard University Press Cancer Stem Cells
Book SynopsisA new therapeutic strategy could break the stalemate in the war on cancer by targeting not all cancerous cells but the small fraction that lie at the root of cancers. Lucie Laplane offers a comprehensive analysis of cancer stem cell theory, based on an original interdisciplinary approach that combines biology, biomedical history, and philosophy.Trade ReviewLaplane places us right in the center of an urgently important discussion of cancer stem cells and the therapies that should accompany different theories. Her fresh philosophical perspective introduces us to a science in process, where the outcome is unknown and even the terms of debate remain contested. -- Jane Maienschein, author of Embryos under the MicroscopeIf you had doubts about the utility of philosophy for science, read this book. Its lucid exploration of stem cells will convince you that even cancer therapy can benefit from the lights of philosophy. -- Michel Morange, author of A History of Molecular BiologyBuilds a much broader framework for understanding the biology of stem cells of all types…Laplane’s rigorous analyses unveil deep semantic and conceptual problems in the field…Laplane’s stemness framework should be of great value…A philosopher may indeed have straightened out the stem-cell field. -- Hans Clever * Nature *
£31.41
Harvard University Press Body Messages
Book SynopsisWhether classified as regulators of inflammation, metabolism, or other functions, a distinctive set of molecules enables the body to convey information from one cell to another. Giamila Fantuzzi offers a primer on molecular mediators that coordinate complex bodily processes, and explores the consequences of their discovery for modern medicine.Trade ReviewFrom the way our brain thinks to how our body fights germs, the wonders of human life are fundamentally about how our cells and tissues interact. This book is a tour de force about how this communication happens. Examined with passion and insight, a wondrous and important story unfolds about how the human body really works above and beyond the level of individual genes and cells. -- Daniel M. Davis, author of The Compatibility GeneThe scholarship in the book is truly impressive. The interviews are revealing portraits of accomplished scientists. The writing style is lucid and purged of jargon so as to be intelligible to non-scientists. All in all this is a valuable and eminently readable record of the birth and development of a field that has had a dramatic impact on human health. -- Scott Durum, Center for Cancer Research, National Cancer InstituteReading this work will keep the reader current on a substantial number of recent, important studies on cell-to-cell information transfer. The reader will enjoy the personal essays and then want to read more about the scientific contributions. It is fascinating to find a work that is genuinely informative and also a joy to read. No matter the nature of the reader’s background, he or she will learn an immense amount of information from this book. -- F. W. Yow * Choice *
£31.41
Harvard University Press Adaptive Oncogenesis
Book SynopsisJames DeGregori proposes a way of thinking about cancer as a disease of evolutionone in which mutated cells outcompete healthy cells in the ecosystem of the body's tissues. By tying cancer's progression to natural selection and evolved strategies for reproductive success, his theory goes far in explaining who gets cancer, when it appears, and why.Trade ReviewWith engaging prose and an authoritative review of new research, Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us corrects the fundamental attribution error that has focused cancer research on malignant cells and their genes. Adaptive oncogenesis, or ‘EcoOncogenesis,’ shows that the ecosystems surrounding cells are equally important, responsible for creating selection forces that speed or slow the evolution of cancer. With huge implications for prevention and treatment, this book is required reading for cancer researchers and clinicians, and a pleasure read for anyone who appreciates fascinating new science. -- Randolph Nesse, Center for Evolution and Medicine at Arizona State UniversityDeGregori’s vision of cancer as an adaptive ecosystem is insightful and cogent. Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us, an evolutionary explanation for the risk of cancer as we age, is a provocative and refreshing revision of the prevailing gene-centric paradigm. -- Mel Greaves, The Institute of Cancer ResearchTo understand how and why tumors progress, James DeGregori argues that we must apply evolutionary principles to cancer. This book is a seamless discussion of natural evolutionary processes, ranging from longevity in rodents to bacterial antibiotic resistance, and drawing parallels to tumorigenesis. It is a must-read for everyone who wants to understand tumor evolution. -- Kornelia Polyak, Dana-Farber Cancer InstituteTo paraphrase Dobzhansky, cancer only makes sense in the light of evolution. In Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us, DeGregori challenges the commonly held view that it takes time for a normal cell to mutate into a cancer cell. He makes the compelling case that the ability to select against cancer becomes weaker as an individual gets older and the odds of reproduction decline. -- Angelika Amon, Koch Institute for Integrative Cancer Research at MITAt every point in our lives, the body holds the mutations necessary to cause cancer; why then does cancer develop primarily at certain times or after specific exposures? With crisp thinking and engaging prose, James DeGregori’s surprisingly readable book argues that cancer is a disease caused by damaged tissues rather than gene mutations. The implications of this revolutionary work have the potential to change the focus of cancer research, and especially research on cancer prevention, with emphasis on using the body’s own calculus of natural selection to keep cancers in check. -- Garth Sundem, author of Brain Trust: 93 Top Scientists Reveal Lab-Tested Secrets to Surfing, Dating, Dieting, Gambling, Growing Man-Eating Plants, and More!Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us is a highly readable and entertaining book, offering a fascinating new look at cancer through an evolutionary and ecological lens. With novel insights and thoughtful observations, James DeGregori guides his audience through the promise of new ideas, examining novel applications of Darwin’s principles as well as modern technological advancements connected to cancer biology and treatment. -- Robert A. Gatenby, Moffitt Cancer CenterA fresh view on how cancer originates and evolves, challenging the mutation-centric dogma of cancer genesis…An important book, accessible to general readers, teachers, and nonexpert biologists, and a must read for medical doctors and drug developers aiming to design effective cancer therapies, as it introduces the fundamental concept of evolution and tissue homeostasis into therapy design. -- Miguel Costa Coelho * Quarterly Review of Biology *
£34.81
Wiley Basic Molecular and Cell Biology
Book SynopsisThis third, fully revised edition, brings the reader up to date with recent advances made in the study of disease at the molecular and cellular level, and examines the new possibilities for treatment. Subjects covered include methods used in molecular medicine and the polymerase chain reaction.Table of ContentsMolecular and cell biology in clinical medicine; methods in molecular medicine; the polymerase chain reaction - a tool for molecular medicine; an introduction to cells; stem cells in normal growth and disease; cell reproduction; apoptosis (programmed cell death); cell to cell and cell to matrix adhesion; how do receptors at the cell surface transmit signals to the cell interior?; membrane traffic, from cell to clinic; cytoskeleton and disease; the cell nucleus' gene regulation and transcription factors; genes and cancer; human congenital malformations - insights from molecular genetics; molecular genetics of common diseases; impact of molecular biology on clinical genetics; monoclonal antibodies in medicine; production and use of therapeutic agents; gene therapy.
£42.70
John Wiley and Sons Ltd Plant Tropisms
Book SynopsisTropisms, the defined vectorial stimuli, such as gravity, light, touch, humidity gradients, ions, oxygen, and temperature, which provide guidance for plant organ growth, is a rapidly growing and changing field. The last few years have witnessed a true renaissance in the analysis of tropisms.Table of ContentsContributors. Preface. 1. Mechanisms of Gravity Perception in Higher Plants: Aline H. Valster and Elison B. Blancaflor. 1.1 Introduction. 1.2 Identification and characterization of gravity perception sites in plant organs. 1.2.1 Roots. 1.2.2 Hypocotyls and inflorescence stems (dicotyledons). 1.2.3 Cereal pulvini (monocotyledons). 1.3 The Starch-statolith hypothesis. 1.3.1 A variety of plant organs utilize sedimenting amyloplasts to sense gravity. 1.3.2 Amyloplast sedimentation is influenced by the environment and developmental stage of the plant. 1.4 The gravitational pressure model for gravity sensing. 1.5 The cytoskeleton in gravity perception. 1.6 Concluding remarks and future prospects. 1.7 Acknowledgment. 1.8 Literature Cited. 2. Signal Transduction in Gravitropism: Benjamin R. Harrison, Miyo T. Morita, Patrick H. Masson and Masao Tasaka. 2.1 Introduction. 2.2 Gravity signal transduction in roots and above-ground organs. 2.2.1 Do mechano-sensitive ion channels function as gravity receptors?. 2.2.2 Inositol 1,4,5 trisphosphate seems to function in gravity signal transduction. 2.2.3 Do pH changes contribute to gravity signal transduction?. 2.2.4 Proteins implicated in gravity signal transduction. 2.2.5 Global ‘-omic’ approaches to the study of root gravitropism. 2.2.6 Re-localization of auxin transport facilitators or activity regulation?. 2.2.7 Could cytokinin also contribute to the gravitropic signal?. 2.3 Gravity signal transduction in organs that do not grow vertically. 2.4 Acknowledgments. 2.5 Cited Literature. 3. Auxin Transport and the Integration of Gravitropic Growth: Gloria K. Muday and Abidur Rahman. 3.1 Introduction to auxins. 3.2 Auxin transport and its role in plant gravity response. 3.3 Approaches to Identify Proteins that Mediate IAA Efflux. 3.4 Proteins that Mediate IAA Efflux. 3.5 IAA influx carriers and their role in gravitropism. 3.6 Regulation of IAA efflux protein location and activity during gravity response. 3.6.1 Mechanisms that may control localization of IAA efflux carriers. 3.6.2 Regulation of IAA efflux by synthesis and degradation of efflux carriers. 3.6.3 Regulation of auxin transport by reversible protein phosphorylation. 3.6.4 Regulation of auxin transport by flavonoids. 3.6.5 Regulation of auxin transport by other signaling pathways. 3.6.6 Regulation of gravity response by ethylene. 3.7 Overview of the mechanisms of auxin induced growth. 3.8 Conclusions. 3.9 Acknowledgements. 3.10 Cited Literature. 4. Phototropism and its Relationship to Gravitropism: Jack L. Mullen and John Z. Kiss. 4.1 Phototropism: General Description and Distribution. 4.2 Light Perception. 4.3 Signal Transduction and Growth Response. 4.4 Interactions with Gravitropism. 4.5 Importance to Plant Form and Function. 4.6 Conclusions and outlook. 4.7 References. 5. Touch Sensing and Thigmotropism: Gabriele B. Monshausen, Sarah J. Swanson and Simon Gilroy. 5.1 Introduction. 5.2 Plant mechanoresponses. 5.2.1 Specialized touch responses. 5.2.2 Thigmomorphogenesis and thigmotropism. 5.3 General principles of touch perception. 5.3.1 Gating through membrane tension: the mechanoreceptor for hypoosmotic stress in bacteria, MscL. 5.3.2 Gating through tethers: the mechanoreceptor for gentle touch in Caenorhabditis elegans. 5.3.3 Evidence for mechanically gated ion channels in plants. 5.4 Signal transduction in Touch & Gravity Perception. 5.4.1 Ionic signaling. 5.4.2 Ca2+ signaling in the touch and gravity response. 5.5 Insights from transcriptional profiling. 5.6 Interaction of touch and gravity signaling/response. 5.7 Conclusion and Perspectives. 5.8 Acknowledgements. 5.9 Cited Literature. 6. Other Tropisms and their Relationship to Gravitropism: Gladys I. Cassab. 6.1 Introduction. 6.2 Hydrotropism. 6.2.1 Early studies of hydrotoprism. 6.2.2 Genetic analysis of hydrotropism. 6.2.3 Perception of moisture gradients and gravity stimuli by the root cap and the curvature response. 6.2.4 ABA and the hydrotropic response. 6.2.5 Future experiments. 6.3 Electrotropism. 6.4 Chemotropism. 6.5 Thermotropism and oxytropism. 6.6 Traumatropism. 6.7 Overview. 6.8 Acknowledgments. 6.9 Literature cited. 7. Single-Cell Gravitropism and Gravitaxis: Markus Braun and Ruth Hemmersbach. Introduction. 7.1 Definitions of responses to environmental stimuli that optimize the ecological fitness of single-cell organisms. 7.2 Occurrence and significance of gravitaxis in single-cell systems. 7.3 Significance of gravitropism in single-cell systems. 7.4 What makes a cell a biological gravity sensor?. 7.5 Gravity susception - the initial physical step of gravity sensing. 7.6 Susception in the statolith-based systems of Chara. 7.7 Susception in the statolith-based system Loxodes. 7.8 Susception in the protoplast-based systems of Euglena and Paramecium. 7.9 Graviperception in the statolith-based systems of Chara. 7.10 Graviperception in the statolith-based system Loxodes. 7.11 Graviperception in the protoplast-based systems Paramecium and Euglena. 7.12 Signal transduction pathways and graviresponse mechanisms in the statolith-based systems of Chara. 7.13 Signal transduction pathways and graviresponse mechanisms in Euglena and Paramecium. 7.14 Conclusions. 7.15 Acknowledgements. 7.18 Cited Literature. 8. Space-Based Research on Plant Tropisms: Melanie J. Correll and John Z. Kiss. 8.1 Introduction - the variety of plant movements. 8.2 The microgravity environment. 8.3 Ground-based studies: mitigating the effects of gravity. 8.4 Gravitropism. 8.4.1 Gravitropism: gravity perception. 8.4.2 Gravitropism: signal transduction. 8.4.3 Gravitropism: the curving response. 8.5 Phototropism. 8.6 Hydrotropism, autotropism and oxytropism. 8.7 Studies of other plant movements in microgravity. 8.8 Spaceflight hardware used to study tropisms. 8.9 Future outlook and prospects. 8.10 Cited Literature. . 9. Plan(t)s for Space Exploration: Christopher S. Brown, Heike Winter Sederoff, Eric Davies, Robert J. Ferl, and Bratislav Stankovic. Introduction. 9.1 Human missions to space. 9.2 Life support. 9.3 Genomics and space exploration. 9.4 Nanotechnology. 9.5 Sensors, biosensors and intelligent machines. 9.6 Plan(t)s for space exploration. 9.7 Imagine…. 9.8 Literature cited
£223.16
Springer-Verlag New York Inc. Pericytes
Book SynopsisTable of ContentsPreface…Table of Contents…Contributing Authors…1. Visualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture Roshini Prakash, Nikita Shivani Thareja, Thomas S. Carmichael, Raymond L. Barnhill, Claire Lugassy, and Laurent A. Bentolila2. Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu MiceJames E. Baily and William C.W. Chen3. Assessment of Pericyte Phenotype by Flow CytometryN. S. Khan, C.C. West, F. Rossi, and M. Crisan4. Characterization of Pericyte Phenotype In Vivo by ImmunohistochemistryC.C. West, N.S. Khan, and M. Crisan5. A Metabolomics Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures E.V. Alakpa, C.C. West, L. Goldie, M. Harper, K. Burgess, R. Ulijn, and M.J. Dalby6. Pericyte Ontogeny: The Use of Chimeras to Track a Cell Lineage of Diverse Germ Line OriginsHeather C. Etchevers7. Imaging Pericytes and the Regulation of Cerebral Blood FlowKatie Boyd, Matthew Hammond-Haley, Rozan Vroman, and Catherine Hall8. Derivation of Pericytes from Human Pluripotent Stem CellsAyelet Dar and Joseph Itskovitz-Eldor9. Assessing the Bone-Forming Potential of Pericytes Carolyn A. Meyers, Chenchao Wang, Jiajia Xu, Hsin Chuan Pan, Jia Shen, Kang Ting, Chia Soo, Bruno Peault, and Aaron W. James10. Manipulating Pericyte Function with MicroRNAsMilena Vitiello, Ben Cathcart, Andrea Caporali, and Marco Meloni11. Isolation of Mesoangioblasts: A Subset of Pericytes with Strong Myogenic Potential from Mammals Giorgia Giacomazzi, Gaia Giovannelli, Alessio Rotini, Domiziana Costamagna, Mattia Quattrocelli, and Maurilio Sampaolesi12. Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes Ania Stefanska, Angela Briski, Nusrat Khan, Christopher Kenyon, John J. Mullins, and Bruno Péault13. Metastatic Dissemination Mimicked in a Multicellular Transwell AssayCourtney König and Anja RungeSubject Index List…
£98.99
Springer-Verlag New York Inc. Craniofacial Development
Book SynopsisThis volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tiTable of Contents1. Using Caenorhabditis elegans as a model for mechanistic insights of craniofacial development Michael Gruss and Ann K. Corsi 2. Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial DevelopmentNishanthi Mathiyalagan and Sebastian Dworkin 3. Elucidation of Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-Mount In Situ Hybridization Joshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam 4. Visualization of the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining Joshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam 5. Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue M. E. de Vries and S. Dworkin 6. Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion Jack D. Morgan and Jeremy B. A. Green 7. Single cell RNA-seq: Cell Isolation and Data Analysis Val Yianni and Paul T Sharpe 8. Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9 Priyanka Kumari, Morgan Sturgeon, Gregory Bonde, and Robert A. Cornell 9. Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures Mustafa Mian, Jenny Tan, Robin Yong, Ruth Williams, Agatha Labrinidis, Peter J. Anderson, and Sarbin Ranjitkar 10. Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull Jenny Tan, Agatha Labrinidis, Ruth Williams, Mustafa Mian, Peter J. Anderson, and Sarbin Ranjitkar 11. Characterization of mammalian in vivo enhancers using mouse transgenesis and CRISPR genome editing Marco Osterwalder, Stella Tran, Riana D. Hunter, Eman M. Meky, Kianna von Maydell, Anne N. Harrington, Janeth Godoy, Catherine S. Novak, Ingrid Plajzer-Frick, Yiwen Zhu, Jennifer A. Akiyama, Veena Afzal, Evgeny Z. Kvon, Len A. Pennacchio, Diane E. Dickel, and Axel Visel 12. Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton Daniela M. Roth, Lakshmi Puttagunta, and Daniel Graf 13. Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development Jacob I. Reynolds, Ross A. Vitek, Peter G. Geiger, and Brian P. Johnson 14. Ex Vivo Culture of Human Cranial Suture Cells Peter J. Anderson 15. Scaffolds for Use in Craniofacial Bone Regeneration Katherine R. Hixon, Christopher T. Eberlin, Meghana Pendyala, Angela Alarcon de la Lastra, and Scott A. Sell 16. Mandible Explant Assay for The Analysis of Meckel’s Cartilage Development Sophie Wiszniak and Quenten Schwarz 17. High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models A. Huysseune, M. Soenens, J.-Y. Sire, and P.E. Witten 18. Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration Emma L. Moore and Paul A. Trainor 19. Salivary Gland Development In Culture Marcia Gaete, Tathyane H. N. Teshima, Lemonia Chatzeli, and Abigail S. Tucker 20. Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis Sarah Constantine and Piotr Niznik
£161.99
Springer-Verlag New York Inc. Plasmodesmata
Book SynopsisThis fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooTable of ContentsPart I: Overview Articles 1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development Philip Kirk and Yoselin Benitez-Alfonso 2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses Caiping Huang and Manfred Heinlein Part II: Structure and Distribution of Plasmodesmata 3. Plasmodesmata Ultrastructure Determination Using Electron Tomography Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer 4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska 5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots Andrea Paterlini and Ilya Belevich 6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith 7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy Florence R. Danila 8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy Kirsten Knox Part III: Compositional Analysis of Plasmodesmata 9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata Caiping Huang, Jerôme Mutterer, and Manfred Heinlein 10. Immunofluorescence Detection of Callose in Plant Tissue Sections Sam Amsbury and Yoselin Benitez-Alfonso 11. Callose Detection and Quantification at Plasmodesmata in Bryophytes Arthur Muller, Tomomichi Fujita, and Yoan Coudert 12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison 13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons Verena Kriechbaumer and Stanley W. Botchway 14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer Patrick Blümke, Vicky Howe, and Rüdiger Simon 15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy Joseph F. McKenna 16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering Ajeet Chaudhary and Kay Schneitz Part IV: Functional Analysis of Plasmodesmata 17. Quantification of Cell-to-Cell Connectivity Using Particle Bombardment Estee E. Tee, Sebastian Samwald, and Christine Faulkner 18. Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay Jessica C. Fernandez and Tessa M. Burch-Smith 19. Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay Wilson Horner and Jacob O. Brunkard 20. An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins Frank Machin, Yağmur Hasbioğlu, and Friedrich Kragler 21. Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP Min Diao and Shanjin Huang 22. Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes Takumi Tomoi, Yoan Coudert, and Tomomichi Fujita 23. Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability Ekaterina A. Lazareva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergey Y. Morozov, Manfred Heinlein, and Andrey G. Solovyev 24. Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis Justyna Wróbel-Marek, Kamila Godel-Jędrychowska, and Ewa Kurczyńska 25. Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling Lisa Van den Broeck, Mariah Gobble, and Rosangela Sozzani 26. Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction Dawei Yan 27. A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue Munenori Kitagawa and David Jackson Part V: Other Approaches Contributing to Plasmodesmata Research 28. In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG Eduardo J. Peña and Manfred Heinlein 29. Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM) Michael Fuchs and Jan U. Lohmann 30. More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight Eva E. Deinum 31. Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis Gwendolyn V. Davis, Richard S. Smith, and George W. Bassel
£170.99
Springer-Verlag New York Inc. cAMP Signaling
Book SynopsisThis volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, cAMP Signaling: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers who are interested in learTable of ContentsPreface…Table of Contents...Contributing Authors…1. Real-Time Measurements of Intracellular cAMP Gradients using FRET-Based cAMP NanorulersCharlotte Konrad, Martin J. Lohse, and Andreas Bock2. Assaying Protein Kinase A Activity using a FRET-Based Sensor Purified from Mammalian CellsAshton J. Curtis, Ryan S. Dowsell, and Matthew G. Gold3. MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRETMasoud Ramuz, Ivan Diakonov, Chris Dunsby, and Julia Gorelik4. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal ActivityThilo Henss, Martin Schneider, Dennis Vettkötter, Wagner Steuer Costa, Jana F. Liewald, and Alexander Gottschalk5. Imaging the cAMP Signaling Microdomain of the Primary Cilium using Targeted FRET-Based BiosensorsDanielle T. Arena and Aldebaran M. Hofer6. Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity via Heterologous Expression in Fission YeastMarek Domin and Charles S. Hoffman7. Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET SensorsOlga Kukk, Jeffrey Klarenbeek, and Kees Jalink8. Disruptors of AKAP-Dependent Protein-Protein InteractionsRyan Walker-Gray, Tamara Pallien, Duncan C. Miller, Andreas Oder, Martin Neuenschwander, Jens Peter von Kries, Sebastian Diecke, and Enno Klussmann9. Micro-2D Cell Culture for cAMP Measurements using FRET Reporters in Human iPSC-Derived Cardiomyocytes Andreas Koschinski and Manuela Zaccolo10. Automated Image Analysis of FRET Signals for Subcellular cAMP QuantificationSilas J. Leavesley, Naga Annamdevula, Santina Johnson, DJ Pleshinger, and Thomas C. Rich11. In Vivo cAMP Dynamics in Drosophila Larval NeuronsIsabella Maiellaro12. Live Cell Imaging of Cyclic Nucleotides in Human CardiomyocytesKira Beneke and Cristina E. Molina13. Optogenetic Control of Heart Rhythm: Lightly Guiding the Cardiac PaceLolita Dokshokova, Nicola Pianca, Tania Zaglia, and Marco Mongillo14. Live Imaging of cAMP Signaling in D. discoideum Based on a Bright Bioluminescent Protein, Nano-LanternKazuki Horikawa and Takeharu Nagai15. Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMPRoberta Kurelić and Viacheslav O. Nikolaev16. How to Make the CUTiest Sensor in 3 Simple Steps for Computational PedestriansFlorencia Klein, Cecilia Abreu, and Sergio Pantano17. Ion-Channel Based Reporters for cAMP DetectionThomas C. Rich, Wenkuan Xin, Silas J. Leavesley, C. Michael Francis, and Mark Taylor18. Quantitative Phosphoproteomics to Study cAMP SignallingKatharina Schleicher, Svenja Hester, Monika Stegmann, and Manuela Zaccolo 19. Biochemical Analysis of AKAP-Anchored PKA Signaling ComplexesDominic P. Byrne, Mitchell H. Omar, Eileen J. Kennedy, Patrick A. Eyers, and John D. Scott20. Fluorescent Translocation Reporters for Sub-Plasma Membrane cAMP ImagingOleg Dyachok, Yunjian Xu, Olof Idevall-Hagren, and Anders Tengholm21. A Live-Cell Imaging Assay for Nuclear Entry of cAMP-Dependent Protein Kinase Catalytic Subunits Stimulated by Endogenous GPCR Activation Grace E. Peng and Mark von Zastrow22. Measuring Spatiotemporal cAMP Dynamics within an Endogenous Signaling Compartment using FluoSTEP-ICUEJulia C. Hardy, Sohum Mehta, and Jin ZhangSubject Index List…
£143.99
Springer-Verlag New York Inc. TGFBeta Signaling
Book SynopsisThis detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-ß) and other cell signaling knowledge.Table of Contents1. Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot Difan Deng and Zhike Zi 2. Fast Quantitation of TGF-β Signaling Using Adenoviral Reporter Adilson Fonseca Teixeira, Josie Iaria, and Hong-Jian Zhu 3. Complex Formation among TGF-b Receptors in Live Cell Membranes Measured by Patch-FRAP Szabina Szófia Szilágyi, Orit Gutman, and Yoav I. Henis 4. Branched Proximity Hybridization Assay for the Quantification of Nanoscale Protein-Protein Proximity Jianying Yang 5. Visualizing Dynamic Changes during TGF-β-Induced Epithelial to Mesenchymal Transition Abhishek Sinha, Pranav Mehta, Chuannan Fan, Jing Zhang, Dieuwke L. Marvin, Maarten van Dinther, Laila Ritsma, Pouyan E. Boukany, and Peter ten Dijke 6. Establishment of Embryonic Zebrafish Xenograft Assays to Investigate TGF-β Family Signaling in Human Breast Cancer Progression Chao Li, Jin Ma, Arwin Groenewoud, Jiang Ren, Sijia Liu, B. Ewa Snaar-Jagalska, and Peter ten Dijke 7. Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling Zixin Huang and Alexander Loewer 8. CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-β Pathway Regulators Nina Frey and Gerald Schwank 9. Optogenetic Control of TGF-β Signaling Yuchao Li and Zhike Zi 10. Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-β Signaling in Mouse Embryonic Stem Cells Fabien Furfaro, Carine Vias, and Benoit Sorre 11. Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network Leijun Ye and Chunhe Li 12. Discrete Logic Modeling of Cell Signaling Pathways Nensi Ikonomi, Silke D. Werle, Julian D. Schwab, and Hans A. Kestler 13. Mining of Single-Cell Signaling Time-Series for Dynamic Phenotypes with Clustering Maciej Dobrzyński, Marc-Antoine Jacques, and Olivier Pertz 14. Automated Classification of Cellular Phenotypes Using Machine Learning in Cellprofiler and CellProfiler Analyst Marja Kornhuber and Sebastian Dunst 15. Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs Zeyu Liu, Adrian Ramirez, and Xuedong Liu
£151.99
Springer-Verlag New York Inc. Celebrating J.D. Murrays Contributions to
Book SynopsisThe Bulletin of Mathematical Biology, the official journal of the Society for Mathematical Biology, disseminates original research findings and other information relevant to the interface of biology and the mathematical sciences. Contributions should have relevance to both fields. In order to accommodate the broad scope of new developments, the journal accepts a variety of contributions, including: Original research articles focused on new biological insights gained with the help of tools from the mathematical sciences or new mathematical tools and methods with demonstrated applicability to biological investigations Research in mathematical biology education Reviews Commentaries Perspectives, and contributions that discuss issues important to the profession All contributions are peer-reviewed.
£67.49
Springer-Verlag New York Inc. The Plant Cytoskeleton
Book SynopsisThis detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.Table of Contents1. Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy Sharol Schmidt-Marcec, Austin Ross, and Andrei Smertenko 2. Actin: Static and Dynamic Studies Huaqiang Ruan, Sha Zhang, Yi Zhang, and Haiyun Ren 3. 3D Visualization of Microtubules in Epidermal Pavement Cells Amir J. Bidhendi, Bara Altartouri, and Anja Geitmann 4. Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells Sandra Klemm, Jonas Buhl, Birgit Möller, and Katharina Bürstenbinder 5. Single Cell Confinement Methods to Study Plant Cytoskeleton Pauline Durand-Smet, Antoine Chevallier, Léia Colin, Alice Malivert, Isaty Melogno, and Olivier Hamant 6. Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures Eduard Belausov, Vikas Dwivedi, Sela Yechezkel, Sefi Bar-Sinai, and Einat Sadot 7. Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography Janice Pennington and Marisa S. Otegui 8. Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana) Yuh-Ru Julie Lee, Calvin H. Huang, and Bo Liu 9. Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis Sabine Müller 10. Expansion Microscopy of Plant Cells (PlantExM) Timothy J. Hawkins, Joanne L. Robson, Bethany Cole, and Simon J. Bush 11. Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens Mari W. Yoshida and Elena Kozgunova 12. Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens Yuji Hiwatashi and Takashi Murata 13. Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools Fatima Cvrčková and Radek Bezvoda 14. Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata Zoe Barr and Jens Tilsner 15. Studying Nuclear Dynamics in Response to Actin Disruption In Planta Joseph F. McKenna and Katja Graumann 16. Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination Lucie Riglet and Isabelle Fobis-Loisy 17. Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes Lei Zhu and Ying Fu 18. Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study Teresa Braga, Fernando Vaz Dias, Marta Fratini, Susana Serrazina, Ingo Heilmann, and Rui Malhó 19. Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis Liru Dou, Xiangfeng Wang, and Tonglin Mao 20. Imaging of Cortical Microtubules in Plants under Salt Stress Shuwei Wang, Liyuan Xu, Changjiang Li, Lei Zhu, Ying Fu, and Yan Guo 21. Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli Bingxiao Wang, Minxia Zou, Qing Pan, and Jiejie Li 22. Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation Amber J. Connerton, Stefan Sassmann, and Michael J. Deeks 23. Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes Qiaonan Lu, Xiaonan Liu, Xiaolu Qu, and Shanjin Huang 24. Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings Felix Ruhnow, Staffan Persson, and René Schneider 25. Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging Guangda Wang, Yanjun Yu, and Zhaosheng Kong 26. Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells Xie Dang, Lilan Zhu, Huibo Ren, and Deshu Lin 27. Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato Zhijing Xu, Xiaolu Qu, Shuang Wu, and Pengwei Wang 28. Light Microscopy Technologies and the Plant Cytoskeleton Timothy J. Hawkins 29. Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton Patrick Duckney and Patrick J. Hussey
£179.99
Springer-Verlag New York Inc. Cell Migration in Three Dimensions
Book SynopsisThis detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration.Table of Contents1. Cell Migration in Three Dimensions Coert Margadant Part I: The Cell Biology of Cell Migration 2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, and David A. Calderwood 3. Probing the ER-Focal Adhesion Link during Cell Migration Noemi Antonella Guadagno and Cinzia Progida 4. Mapping the Localization of Proteins within Filopodia Using FiloMap Guillaume Jacquemet 5. Extended Methods for 2D Confinement Juan Manuel García-Arcos, Kevin Gateau, Larisa Venkova, and Matthieu Piel 6. Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63 Bong Hwan Sung and Alissa M. Weaver 7. Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf Part II: Developmental Model Systems to Assess Cell Migration during Morphogenesis 8. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila Maik C. Bischoff and Sven Bogdan 9. Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells Antonius L. van Boxtel 10. Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure Matteo A. Molè, Gabriel L. Galea, and Andrew J. Copp 11. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila Sofia J. Araújo and Marta Llimargas 12. A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland Edouard Hannezo and Colinda L.G.J. Scheele 13. Analysis of Integrin-Dependent Melanoblast Migration during Development Amanda Haage and Guy Tanentzapf Part III: Cell Migration in Cancers and the Tumor Microenvironment 14. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices David Remy, Anne-Sophie Macé, Philippe Chavrier, and Pedro Monteiro 15. Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion Jian Zhang and Cynthia A. Reinhart-King 16. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids Konstadinos Moissoglu, Stephen J. Lockett, and Stavroula Mili 17. Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices Daan Smits and Antoine A. Khalil 18. Analysis of Collective Migration Patterns within Tumors Ralitza Staneva and Andrew G. Clark 19. An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-Evaluation Ann L.B. Seynhaeve and Timo L.M. ten Hagen Part IV: Blood Vessel Formation and Interactions 20. Angiogenesis Invasion Assay to Study Endothelial Cell Invasion and Sprouting Behavior Yuechao Dong, Florian Alonso, Tiya Jahjah, Isabelle Fremaux, and Elisabeth Génot 21. Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics during Collective Cell Migration Maxime Cammeraat, Marko Popovic, Wendy Stam, and Coert Margadant 22. Analysis of mRNA Subcellular Distribution in Collective Cell Migration Joshua J. Bradbury, Holly E. Lovegrove, Marta Giralt-Pujol, and Shane P. Herbert 23. A Bio-Engineered Model for Studying Vascular-Pericyte Interactions of the Placenta Marta Cherubini and Kristina Haase 24. Analysis of Vascular Morphogenesis in Zebrafish Marvin Hubert and Wiebke Herzog 25. Analysis of Monocyte Recruitment during Inflammation by Intravital Imaging Jose M. Gonzalez-Granado, Alberto Del Monte, Laura Piqueras, Vicente Andres, and Cristina Rius
£107.99
Springer-Verlag New York Inc. Liposomes
Book SynopsisThis third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.Table of Contents1. From olive oil emulsions to COVID-19 Vaccines - Liposomes Came First Volkmar Weissig 2 Preparation of DRV liposomes Sophia G. Antimisiaris 3 Preparation of small unilamellar vesicles using detergent dialysis method Qingyue Zhong and Hongwei Zhang 4 Thin-film hydration followed by extrusion method for liposome preparation Hongwei Zhang 5 Ethanol injection method for liposome preparation Guangsheng Du and Xun Sun 6 Preparation of giant vesicles with mixed single-tail and double-tail lipids Lauren A Lowe and Anna Wang 7 Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing Jung Yeon Han, Zhu Chen, and Don L. DeVoe 8 Preparation of doxorubicin liposomes by remote loading method Jian Chen 9 Magnetic thermosensitive liposomes loaded with Doxorubicin Mohamad Alawak, Alice Abu Dayyih, Ibrahim Awak, Bernd Gutberlet, Konrad Engelhardt, and Udo Bakowsky 10 Preparation and physical characterization of DNA binding cationic liposomes Vaibhav Saxena 11 Tunable pH sensitive lipoplexes Helene Dhotel, Michel Bessodes and Nathalie Mignet 12 Solid Lipid Nanoparticles for Drug Delivery Wei-Chung Luo and Xiuling Lu 13 Stable Discoidal Bicelles – Formulation, Characterization and Functions Ying Liu, Yan Xia, Armin Tahmasbi Rad, Wafa Aresh, Justin M. Fang, and Mu-Ping Nieh 14 The post-insertion method for the preparation of PEGylated liposomes Sherif E. Emam, Nehal E. Elsadek, Taro Shimizua, and Tatsuhiro Ishidaa 15 Click chemistry for liposome surface modification Maria Vittoria Spanedda, Marcella De Giorgi, Béatrice Heurtault, Antoine Kichler, Line Bourel-Bonnet and Benoît Frisch 16 Surface Modification of Liposomes Using Folic Acid Mengran Guo, Zhongshan He, Xi He and Xiangrong Song 17 Preparation and Characterization of Trastuzumab Fab conjugated liposomes (immunoliposomes) Yuhong Xu 18 Pyrophosphorylated cholesterol modified bone-targeting liposomes formulation procedure Yanzhi Liu, Zhenshan Jia, Luoyang Ma, and Dong Wang 19 Method of simultaneous analysis of liposome components using HPTLC/FID Sophia Hatziantoniou and Costas Demetzos 20 HPLC-MS/MS Method for Identification and Quantification of Lipids in Liposomes Yujie Shi and Xiaona Li 21 DPH probe method for liposome-membrane fluidity determination Wei He 22 Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy Anand S. Ubhe 23 Visualization and characterization of liposomes by Atomic Force Microscopy (AFM) Konrad Engelhardt, Eduard Preis and Udo Bakowsky 24 Determination of the sub-cellular distribution of fluorescently labeled liposomes using confocal microscopy Melani A. Solomon 25 Liposome biodistribution via europium complexes Nathalie Mignet and Daniel Scherman 26 Quantification of a fluorescent lipid DOPE-NBD by an HPLC method in biological tissue: application to study liposome’s uptake by human placenta Louise Fliedel, Nathalie Mignet, Thierry Fournier, Karine Andrieux, and Khair Alhareth
£179.99
Humana Cilia
Book SynopsisCombination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium.- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures.- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells.- Expansion Microscopy of Ciliary Proteins.- Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes.- Structural Analysis of Sperm Centrioles Using N-STORM.- A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium.- Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets.- Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging.- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells.- Proximity Mapping of CTable of ContentsPreface…Table of Contents…Contributing Authors… 1. Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway EpitheliumLaure-Emmanuelle Zaragosi, Alizé Gouleau, Margot Delin, Kevin Lebrigand, Marei-Jeanne Arguel, Cedric Girard-Riboulleau, Geraldine Rios, Elisa Redman, Magali Plaisant, Rainer Waldmann, Virginie Magnone, Brice Marcet, Pascal Barbry, and Gilles Ponzio2. SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface CulturesMaria Victoria Humbert, Christopher J. McCormick, and Cosma Mirella Spalluto3. A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured CellsF. Basak Turan, M. Erdem Ercan, and Elif Nur Firat-Karalar4. Expansion Microscopy of Ciliary ProteinsSohyeon Park and Xiaoyu Shi5. Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes Dong Kong, Delgermaa Luvsanjav, and Jadranka Loncarek 6. Structural Analysis of Sperm Centrioles Using N-STORMAbigail Royfman, Sushil Khanal, and Tomer Avidor-Reiss7. A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated EpitheliumNobuhiro Morone, Maria Guerra Martin, Patricia Goggin, Kirk Czymmek, Vito Mennella, and Jaime Llodra Gonzalez8. Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets Aaran Vijayakumaran, Analle Abuammar, Odara Medagedara, Kedar Narayan, and Vito Mennella9. Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell ImagingStefanie Kuhns, Alice Dupont Juhl, Zeinab Anvarian, Daniel Wüstner, Lotte B. Pedersen, and Jens S. Andersen10. Live-Imaging Centriole Amplification in Mouse Brain Multiciliated CellsAmélie-Rose Boudjema, Adel Al Jord, Anne-Iris Lemaître, Marion Faucourt, Nathalie Delgehyr, Nathalie Spassky, and Alice Meunier11. Proximity Mapping of Ciliary Proteins by BioIDMelissa Iazzi, Jonathan St-Germain, Saujanya Acharya, Brian Raught, and Gagan D. Gupta12. Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice using Endogenous Streptavidin/FLAG TagsTina Beyer, Tiago Martins, Jeshmi Jeyabalan Srikaran, Marian Seda, Emma Peskett, Franziska Klose, Katrin Junger, Philip L. Beales, Marius Ueffing, Karsten Boldt, and Dagan Jenkins13. Primary Human Nasal Epithelial Cell CultureMystica Terrance, Tarini Gunawardena, Hong Ouyang, Julie Avolio, Wenming Duan, Sowmya Thanikachalam, and Theo J. Moraes14. BMI1 Transduction of Human Airway Epithelial Cells for Expansion of Proliferation and Differentiation Ruhina Maeshima, Amy Jacobs, Melis T. Dalbay, and Stephen L. Hart15. High Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture ModelsWilliam J. Dawes, Oriane Grant, Sam C. Reitemeier, Sarah Tetlow, Dani Lee, Robert A. Hirst, and Christopher O’Callaghan16. Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis MethodsErika Causa, Ricardo Fradique, and Pietro Cicuta17. Mucociliary Transport Device Construction and Application to Study Mucociliary ClearancePatrick R. Sears and Lawrence E. OstrowskiSubject Index List…
£161.99
John Wiley & Sons Inc Fluorescent Analogs of Biomolecular Building
Book SynopsisFluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.Trade Review"This book provides a thorough overview on the design and application of fluorescent analogs of biomolecular building blocks...The way the book is written makes reading enjoyable and relatively easy for readers who already have some knowledge on the subject as well as for beginners...Overall, the book is very well achieved, and I strongly recommend reading." (Angewandte Chemie International Edition May 2017)Table of ContentsList of Contributors xv Preface xix 1 Fluorescence Spectroscopy 1 Renatus W. Sinkeldam, L. Marcus Wilhelmsson, and Yitzhak Tor 1.1 Fundamentals of Fluorescence Spectroscopy 1 1.2 Common Fluorescence Spectroscopy Techniques 3 1.2.1 Steady-State Fluorescence Spectroscopy 3 1.2.2 Time-Resolved Fluorescence Spectroscopy 5 1.2.3 Fluorescence Anisotropy 6 1.2.4 Resonance Energy Transfer and Quenching 7 1.2.5 Fluorescence Microscopy and Single Molecule Spectroscopy 8 1.2.6 Fluorescence-Based in vivo Imaging 9 1.3 Summary and Perspective 10 References 10 2 Naturally Occurring and Synthetic Fluorescent Biomolecular Building Blocks 15 Renatus W. Sinkeldam and Yitzhak Tor 2.1 Introduction 15 2.2 Naturally Occurring Emissive Biomolecular Building Blocks 16 2.3 Synthetic Fluorescent Analogs of Biomolecular Building Blocks 18 2.3.1 Synthetic Emissive Analogs of Membranes Constituents 19 2.3.2 Synthetic Emissive Analogs of Amino Acids 22 2.3.3 Synthetic Emissive Analogs of Nucleosides 24 2.4 Summary and Perspective 31 References 32 3 Polarized Spectroscopy with Fluorescent Biomolecular Building Blocks 40 Bo Albinsson and Bengt Nordén 3.1 Transition Moments 40 3.2 Linear Dichroism 41 3.3 Magnetic Circular Dichroism 45 3.4 F̈orster Resonance Energy Transfer (FRET) 46 3.5 Fluorescence Anisotropy 47 3.6 Fluorescent Nucleobases 47 3.7 Fluorescent Peptide Chromophores 48 3.8 Site-Specific Linear Dichroism (SSLD) 50 3.9 Single-Molecule Fluorescence Resonance Energy Transfer (smFRET) 50 3.10 Single-Molecule Fluorescence-Detected Linear Dichroism (smFLD) 51 References 53 4 Fluorescent Proteins: The Show Must go on! 55 Gregor Jung 4.1 Introduction 55 4.2 Historical Survey 55 4.3 Photophysical Properties 57 4.3.1 Absorption Properties and Color Hue Modification 57 4.3.2 Chromophore Formation 61 4.3.3 Fluorescence Color and Dynamics 64 4.3.4 Directional Properties along with Optical Transitions 68 4.3.5 Energy Transfer and Energy Migration 69 4.4 Photochemical Reactions 71 4.4.1 Excited-state Proton Transfer (ESPT) 71 4.4.2 Isomerization Reactions: Reversible Photoswitching 73 4.4.3 Photoconversion: Irreversible Bond Rupture 74 4.4.4 Other Photochemical Reactions 75 4.5 Ion Sensitivity 75 4.5.1 Ground-State Equilibria of Protonation States 75 4.5.2 Quenching by Small Ions 76 4.6 Relation Microscopy–Spectroscopy for Fluorescent Proteins 77 4.6.1 Brightness Alteration from Cuvette to Microscopic Experiments 77 4.6.2 Lessons from Microspectrometry 78 4.6.3 Tools for Advanced Microscopic Techniques 79 4.7 Prospects and Outlook 82 Acknowledgments 82 References 82 5 Design and Application of Autofluorescent Proteins by Biological Incorporation of Intrinsically Fluorescent Noncanonical Amino Acids 91 Patrick M. Durkin and Nediljko Budisa 5.1 Introduction 91 5.2 Design and Synthesis of Fluorescent Building Blocks in Proteins 97 5.2.1 Extrinsic Fluorescent Labels 97 5.2.2 Intrinsic Fluorescent Labels 98 5.2.3 Extrinsic Labels Chemically Ligated using Cycloaddition Chemistry 108 5.2.4 Modification of the Genetic Code to Incorporate ncAAs 109 5.3 Application of Fluorescent Building Blocks in Proteins 111 5.3.1 Azatryptophans 111 5.3.2 FlAsH-EDT2 Extrinsic Labeling System 112 5.3.3 Huisgen Dipolar Cycloaddition System 114 5.4 Conclusions 117 5.5 Prospects and Outlook 118 5.5.1 Heteroatom-Containing Trp Analogs 119 5.5.2 Expanded Genetic Code – Orthogonal Pairs 119 Acknowledgments 120 References 120 6 Fluoromodules: Fluorescent Dye–Protein Complexes for Genetically Encodable Labels 124 Bruce A. Armitage 6.1 Introduction 124 6.2 Fluoromodule Development and Characterization 126 6.2.1 Fluorogenic Dyes 128 6.2.2 Fluorogen-Activating Protein (FAP) Optimization 131 6.2.3 Fluoromodule Recycling 132 6.3 Implementation 132 6.3.1 Fusion Constructs for Protein Tagging 132 6.3.2 Protein Tagging and pH Sensing 133 6.3.3 Super-Resolution Imaging 133 6.3.4 Protease Biosensors 133 6.4 Conclusions 134 6.5 Prospects and Outlook 134 Acknowledgments 134 References 134 7 Design of Environmentally Sensitive Fluorescent Nucleosides and their Applications 137 Subhendu Sekhar Bag and Isao Saito 7.1 Introduction 137 7.1.1 Solvatochromic Fluorophores 138 7.1.2 Origin of Solvatochromism 139 7.2 Solvatochromic Fluorescent Nucleoside Analogs 140 7.2.1 Designing Criteria for Solvatochromic Fluorescent Nucleosides 140 7.3 Fluorescently Labeled Nucleosides and Oligonucleotide Probes: Covalent Attachment of Solvatochromic Fluorophores Onto the Natural Bases 141 7.3.1 Base-Discriminating Fluorescent Nucleosides (BDF) 142 7.4 Nucleosides with Dual Fluorescence for Monitoring DNA Hybridization 153 7.5 Approach for Developing Environmentally Sensitive Fluorescent (ESF) Nucleosides 154 7.5.1 Concept for Designing ESF Nucleosides 154 7.5.2 Examples and Photophysical Properties of ESF Nucleosides 156 7.6 Base-Selective Fluorescent ESF Probe 163 7.6.1 Cytosine Selective ESF Probe 163 7.6.2 Thymine Selective Fluorescent ESF Probe 163 7.6.3 Specific Detection of Adenine by Exciplex Formation with Donor-Substituted ESF Guanosine 165 7.7 Molecular Beacon (MB) and ESF Nucleosides 167 7.7.1 Ends-Free and Self-Quenched MB 167 7.7.2 Single-Stranded Molecular Beacon Using ESF Nucleoside in a Bulge Structure 168 7.8 Summary and Future Outlook 169 Acknowledgments 170 References 170 8 Expanding The Nucleic Acid Chemist’s Toolbox: Fluorescent Cytidine Analogs 174 Kirby Chicas and Robert H.E. Hudson 8.1 Introduction 174 8.2 Design and Characterization of Fluorescent C Analogs 176 8.2.1 1,3-Diaza-2-Oxophenothiazine (tC) 177 8.2.2 1,3-Diaza-2-Oxophenoxazine (tCO) 178 8.2.3 7-Nitro-1,3-Diaza-2-Oxophenothiazine (tCnitro) 179 8.2.4 G-Clamp and 8-oxoG-Clamp 179 8.2.5 Ç and Çf 181 8.2.6 Benzopyridopyrimidine (BPP) 182 8.2.7 Napthopyridopyrimidine (NPP) 183 8.2.8 dChpp 183 8.2.9 dChpd, dCmpp, dCtpp, dCppp 184 8.2.10 dCPPI 184 8.2.11 dxC 185 8.2.12 rxC 186 8.2.13 Methylpyrrolo-dC (MepdC) 186 8.2.14 5-(Fur-2-yl)-2′-Deoxycytidine (CFU) 187 8.2.15 Thiophen-2-yl pC 187 8.2.16 Thiophene Fused pC 188 8.2.17 Thieno[3,4-d]-Cytidine (thC) 189 8.2.18 Triazole Appended 190 8.3 Implementation 190 8.3.1 PNA 192 8.3.2 DNA 196 8.3.3 RNA 200 8.4 Conclusions 202 8.5 Prospects and Outlook 202 Acknowledgments 203 References 203 9 Synthesis and Fluorescence Properties of Nucleosides with Pyrimidopyrimidine-Type Base Moieties 208 Kohji Seio, Takashi Kanamori, Akihiro Ohkubo, and Mitsuo Sekine 9.1 Introduction 209 9.2 Discovery, Design, and Synthesis of Pyrimidopyrimidine Nucleosides 209 9.2.1 Synthesis and Fluorescence Properties of dChpp 209 9.2.2 Design, Synthesis, and Fluorescence Properties of dCPPP, dCPPI, and dCPPI Derivatives 212 9.2.3 Fluorescence Properties of the Oligonucleotides Containing dCPPI 213 9.3 Implementation 215 9.3.1 Application to a DNA Triplex System 215 9.3.2 Double Labeling of an Oligonucleotide with dCPPI and 2-Aminopurine 219 9.4 Conclusions 220 9.5 Prospects and Outlook 221 References 221 10 Förster Resonance Energy Transfer (FRET) Between Nucleobase Analogues – a Tool for Detailed Structure and Dynamics Investigations 224 L. Marcus Wilhelmsson 10.1 Introduction 224 10.2 The Tricyclic Cytosine Family 226 10.2.1 Structural Aspects, Dynamics, and Ability to Serve as Cytosine Analogs 228 10.2.2 Photophysical Properties 231 10.3 Development of the First Nucleic Acid Base Analog FRET Pair 234 10.3.1 The Donor–Acceptor Pair tCO –tCnitro 235 10.3.2 Applications of Tricyclic Cytosines in FRET Measurements 237 10.4 Conclusions 238 10.5 Prospects and Outlook 238 Acknowledgments 239 References 239 11 Fluorescent Purine Analogs that Shed Light on DNA Structure and Function 242 Anaëlle Dumas, Guillaume Mata, and Nathan W. Luedtke 11.1 Introduction 242 11.2 Design, Photophysical Properties, and Applications of Purine Mimics 244 11.2.1 Early Examples of Fluorescent Purine Mimics 245 11.2.2 Chromophore-Conjugated Purine Analogs 246 11.2.3 Pteridines 250 11.2.4 Isomorphic Purine Analogs 251 11.2.5 Fused-Ring Purine Analogs 252 11.2.6 Substituted Purine Derivatives 253 11.3 Implementation 258 11.3.1 Probing G-Quadruplex Structures with 2PyG 259 11.3.2 Energy Transfer Quantification 261 11.3.3 Metal-Ion Localization to N7 264 11.4 Conclusions 265 11.5 Prospects and Outlook 265 Appendix 268 References 268 12 Design and Photophysics of Environmentally Sensitive Isomorphic Fluorescent Nucleosides 276 Renatus W. Sinkeldam and Yitzhak Tor 12.1 Introduction 276 12.2 Designing Environmentally Sensitive Emissive Nucleosides 279 12.2.1 Structural and Electronic Elements that Impart Environmental Sensitivity 279 12.2.2 Sensitivity to Polarity 279 12.2.3 Sensitivity to Viscosity 281 12.2.4 Sensitivity to pH 282 12.3 Two Isomorphic Environmentally Sensitive Designs 282 12.4 Probing Environmental Sensitivity 283 12.4.1 Probing Sensitivity to Polarity 283 12.4.2 Probing Sensitivity to Viscosity 286 12.4.3 Probing Sensitivity to pH 288 12.5 Recent Advancements in Isomorphic Fluorescent Nucleoside Analogs 291 12.6 Summary 293 12.7 Prospects and Outlook 294 Acknowledgments 294 References 294 13 Site-Specific Fluorescent Labeling of Nucleic Acids by Genetic Alphabet Expansion Using Unnatural Base Pair Systems 297 Michiko Kimoto, Rie Yamashige, and Ichiro Hirao 13.1 Introduction 297 13.2 Development of Unnatural Base Pair Systems and Their Applications 299 13.2.1 Site-Specific Fluorescent Labeling of DNA by Unnatural Base Pair Replication Systems 301 13.2.2 Site-Specific Fluorescent Labeling of RNA by Unnatural Base Pair Transcription Systems 307 13.3 Implementation 310 13.3.1 Fluorescence Sensor System Using an RNA Aptamer by Fluorophore-Linked y Labeling 310 13.3.2 Local Structure Analyses of Functional RNA Molecules by s Labeling 313 13.4 Conclusions 315 13.5 Prospects and Outlook 316 Acknowledgments 317 References 317 14 Fluorescent C-Nucleosides and their Oligomeric Assemblies 320 Pete Crisalli and Eric T. Kool 14.1 Introduction 320 14.2 Design, Synthesis, Characterization, and Properties of Fluorescent C-Glycoside Monomers 322 14.2.1 Design of Fluorescent C-Glycoside Monomers 322 14.2.2 Synthesis of Fluorescent C-Glycoside Monomers 323 14.2.3 Characterization and Properties of Fluorescent C-glycoside Monomers 325 14.3 Implementation of Fluorescent C-Glycoside Monomers 327 14.3.1 Environmentally Sensitive Fluorophores 327 14.3.2 Pyrene Nucleoside in DNA Applications 330 14.4 Oligomers of Fluorescent C-Glycosides: Design, Synthesis, and Properties 335 14.4.1 Design of Fluorescent C-Glycoside Oligomers 335 14.4.2 Synthesis of Fluorescent C-Glycoside Oligomers 336 14.4.3 Characterization and Properties of Fluorescent C-Glycoside Oligomers 337 14.5 Implementation of Fluorescent C-Glycoside Oligomers 342 14.5.1 ODFs as Chemosensors in the Solution State 342 14.5.2 ODFs as Sensors in the Solid State 347 14.5.3 Alternative Designs of Oligomeric Fluorescent Glycosides 351 14.5.4 General Conclusions: Oligomers of Fluorescent C-glycosides 352 14.6 Conclusions 353 14.7 Prospects and Outlook 353 Acknowledgments 354 References 354 15 Membrane Fluorescent Probes: Insights and Perspectives 356 Amitabha Chattopadhyay, Sandeep Shrivastava, and Arunima Chaudhuri Abbreviations 356 15.1 Introduction 357 15.2 NBD-Labeled Lipids: Monitoring Slow Solvent Relaxation in Membranes 358 15.3 n-AS Membrane Probes: Depth-Dependent Solvent Relaxation as Membrane Dipstick 359 15.4 Pyrene: a Multiparameter Membrane Probe 362 15.5 Conclusion and Future Perspectives 362 Acknowledgments 364 References 364 16 Lipophilic Fluorescent Probes: Guides to the Complexity of Lipid Membranes 367 Marek Cebecauer and Radek Šachl 16.1 Introduction 367 16.2 Lipids, Lipid Bilayers, and Biomembranes 368 16.3 Lipid Phases, Phase Separation, and Lipid Ordering 370 16.4 Fluorescent Probes for Membrane Studies 370 16.4.1 Fluorescently Labeled Lipids 371 16.4.2 Environment-Sensitive Membrane Probes 373 16.4.3 Specialized Techniques Using Fluorescent Probes to Investigate Membrane Properties 380 16.5 Conclusions 386 16.6 Prospects and Outlook 386 Acknowledgments 386 References 387 17 Fluorescent Neurotransmitter Analogs 393 James N. Wilson 17.1 Introduction 393 17.1.1 Structure of Neurotransmitters 393 17.1.2 Regulation of Neurotransmitters 394 17.1.3 Native Fluorescence of Neurotransmitters 395 17.1.4 Fluorescent Histochemical Techniques 396 17.2 Design and Optical Properties of Fluorescent Neurotransmitters 397 17.2.1 Early Examples 397 17.2.2 Recent Examples 398 17.3 Applications of Fluorescent Neurotransmitters 400 17.3.1 Probing Binding Pockets with Fluorescent Neurotransmitters 400 17.3.2 Imaging Transport and Release of Fluorescent Neurotransmitters 401 17.3.3 Enzyme Substrates 403 17.4 Conclusions 404 17.5 Prospects and Outlook 405 Acknowledgments 405 References 406 Index 409
£136.76
John Wiley and Sons Ltd Plant Cell Wall Patterning and Cell Shape
Book SynopsisPlant Cell Wall Patterning and Cell Shape is among the first books to take a holistic look at the cell wall?s complex role in plant growth and development. This study examines how cell walls dictate cell shape, their effects on plant development, and researchers? latest perceptions of cell wall pattering.Table of ContentsList of Contributors vii Preface xiii Section 1 Factors Controlling Plant Cell Wall Patterning 1 1 The Biosynthesis and Function of Polysaccharide Components of the Plant Cell Wall 3 Ryusuke Yokoyama, Naoki Shinohara, Rin Asaoka, Hideki Narukawa and Kazuhiko Nishitani 2 Regulation of Cell Wall Formation by Membrane Traffic 35 Clara Sánchez-Rodríguez and Staffan Persson 3 A Blueprint for Cellulose Biosynthesis, Deposition, and Regulation in Plants 65 Ian S. Wallace and Chris R. Somerville 4 Cortical Microtubule Array Organization and Plant Cell Morphogenesis 97 Sidney L. Shaw and Laura Vineyard 5 Actin Filament Dynamics and their Role in Plant Cell Expansion 127 Jiejie Li, Ruthie Arieti and Christopher J. Staiger Section 2 Cellular Mechanisms Underlying Various Cell Shapes 163 6 The Regulation of Cell Shape Formation by ROP-dependent Auxin Signaling 165 Shingo Nagawa and Zhenbiao Yang 7 Xylem Cell Wall Pattern Formation Regulated by Microtubule-associated Proteins and ROP GTPases 191 Yoshihisa Oda and Hiroo Fukuda 8 ROP Signaling and the Cytoskeleton in Pollen Tube Growth 215 Lei Zhu and Ying Fu 9 Phosphoinositide Signaling in Root Hair Tip Growth 239 Hiroaki Kusano, Rumi Tominaga, Takuji Wada, Mariko Kato and Takashi Aoyama 10 Arabidopsis Trichome Morphogenesis and the Role of Microtubules in Controlling Trichome Branch Formation 269 M. David Marks 11 Transfer Cells: Novel Cell Types with Unique Wall Ingrowth Architecture Designed for Optimized Nutrient Transport 287 David W. McCurdy Section 3 Developmental Regulations of Cell Shape 319 12 Regulation of Guard Cell Formation by Integration of Transcriptional and Signaling Regulation 321 Chin-Min Kimmy Ho and Dominique C. Bergmann 13 Transcriptional Regulation of Biosynthesis of Cell Wall Components during Xylem Differentiation 351 Ruiqin Zhong and Zheng-Hua Ye 14 Phloem Cell Development 379 Raffael Lichtenberger, Kaori Furuta-Miyashima, Eva Hellmann and Ykä Helariutta Index 401
£161.06
John Wiley and Sons Ltd Technology Platforms for 3D Cell Culture
Book SynopsisTechnology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.Table of ContentsList of contributors, vii Preface, xi List of abbreviations, xiv 1 An introduction to the third dimension for routine cell culture, 1Antonio Romo‐Morales and Stefan Przyborski Part I: Aggregate‐based technologies 2 Gravity‐enforced microtissue engineering, 23Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm 3 Physiologically relevant spheroid models for three‐dimensionalcell culture, 50Nicole A. Slawny and MaryAnn Labant 4 NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture, 74Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman 5 Micro‐moulded non‐adhesive hydrogels to form multicellular microtissues – the 3D Petri Dish®, 97Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan 6 Organotypic microtissues on an air‐liquid interface, 123Lars E. Sundstrom, Igor Charvet and Luc Stoppini Part II: Hydrogels 7 Materials and assay systems used for three‐dimensional cell culture, 145Suparna Sanyal and Marshall Kosovsky 8 HyStem®, a customisable hyaluronan‐based hydrogel matrix for 3D cell culture, 173T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach‐Carson and D. D. Carson 9 3‐D Life biomimetic hydrogels: A modular system for cell environment design, 197Brigitte M. Angres and Helmut Wurst Part III: Scaffolds 10 Alvetex®, a highly porous polystyrene scaffold for routine three‐dimensional cell culture, 225Antonio Romo‐Morales, Eleanor Knight and Stefan Przyborski 11 CelluSponge™ and Go Matrix as innovative three‐dimensional cell culture platforms, 250Bramasta Nugraha 12 Mimetix® electrospun scaffold: An easy‐to‐use tool for 3D cell culture in drug discovery and regenerative medicine, 284Robert J. McKean and Elena Heister Part IV: 3D bioreactor technologies 13 Quasi Vivo® bioreactor technology, 305J. Malcolm Wilkinson 14 Three‐dimensional cell‐based assays in hollow fibre bioreactors, 327John J. S. Cadwell and William G. Whitford 15 Three‐dimensional engineered tissues for high‐throughput compound screening: Mechanical properties of skin and ageing, 351Michael Conway, Ayla Annac and Tetsuro Wakatsuki 16 Three‐dimensional cell culture in the Rotary Cell Culture System™, 370Stephen S. Navran Glossary, 386 Index, 393
£111.56
John Wiley and Sons Ltd Fundamental Molecular Biology
Book SynopsisFundamental Molecular Biology Discover a focused and up to date exploration of foundational and core concepts in molecular biologyThe newly revised Third Edition of Fundamental Molecular Biology delivers a selective and precise treatment of essential topics in molecular biology perfect for allowing students to develop an accurate understanding of the applications of the field. The book applies the process of discovery-observations, questions, experimental designs, results, and conclusions-with an emphasis on the language of molecular biology. Readers will easily focus on the key ideas they need to succeed in any introductory molecular biology course.Fundamental Molecular Biology provides students with the most up to date techniques and research used by molecular biologists today. Readers of the book will have the support and resources they need to develop a concrete understanding of core and foundational concepts of molecular biology, without beiTable of ContentsChapter 1 The Beginnings of Molecular Biology Chapter 2 The Structure of DNA Chapter 3 The Versatility of RNA Chapter 4 Protein Structure and Folding Chapter 5 Genome Organization and Evolution Chapter 6 DNA Replication and Telomer Maintenance Chapter 7 DNA Repair Pathways Chapter 8 Transcription in Bacteria Chapter 9 Transcription in Eukaryotes Chapter 10 Epigenetic Mechanisms of Gene Regulation Chapter 11 RNA Processing and Posttranscriptional Gene Regulation Chapter 12 The Mechanisms of Translation Chapter 13 Recombinant DNA Technology and Genetically Modified Organisms Chapter 14 Tools for Analyzing Gene Organization, Expression, and Function Chapter 15 Medical Molecular Biology
£130.45
John Wiley & Sons Inc Advanced Surfaces for Stem Cell Research
Book SynopsisThe book outlines first the importance of Extra Cellular Matrix (ECM), which is a natural surface for most of cells. In the following chapters the influence of biological, chemical, mechanical, and physical properties of surfaces in micro and nano-scale on stem cell behavior are discussed including the mechanotransduction. Biomimetic and bioinspired approaches are highlighted for developing microenvironment of several tissues, and surface engineering applications are discussed in tissue engineering, regenerative medicine and different type of biomaterials in various chapters of the book. This book brings together innovative methodologies and strategies adopted in the research and development of Advanced Surfaces in Stem Cell Research. Well-known worldwide researchers deliberate subjects including: Extracellular matrix proteins for stem cell fateThe superficial mechanical and physical properties of matrix microenvironment as stem cell fate regulatorEffects of mechanotransduction on sTable of ContentsPreface xv 1 Extracellular Matrix Proteins for Stem Cell Fate 1 Betül Çelebi-Saltik 1.1 Human Stem Cells, Sources, and Niches 2 1.2 Role of Extrinsic and Intrinsic Factors 5 1.2.1 Shape 5 1.2.2 Topography Regulates Cell Fate 6 1.2.3 Stiffness and Stress 6 1.2.4 Integrins 7 1.2.5 Signaling via Integrins 9 1.3 Extracellular Matrix of the Mesenchyme: Human Bone Marrow 11 1.4 Biomimetic Peptides as Extracellular Matrix Proteins 13 References 15 2 The Superficial Mechanical and Physical Properties of Matrix Microenvironment as Stem Cell Fate Regulator 23 Mohsen Shahrousvand, Gity Mir Mohamad Sadeghi and Ali Salimi 2.1 Introduction 24 2.2 Fabrication of the Microenvironments with Different Properties in Surfaces 25 2.3 Effects of Surface Topography on Stem Cell Behaviors 28 2.4 Role of Substrate Stiffness and Elasticity of Matrix on Cell Culture 31 2.5 Stem Cell Fate Induced by Matrix Stiffness and Its Mechanism 32 2.6 Competition/Compliance between Matrix Stiffness and Other Signals and Their Effect on Stem Cells Fate 33 2.7 Effects of Matrix Stiffness on Stem Cells in Two Dimensions versus Three Dimensions 34 2.8 Effects of External Mechanical Cues on Stem Cell Fate from Surface Interactions Perspective 34 2.9 Conclusions 35 Acknowledgments 36 References 36 3 Effects of Mechanotransduction on Stem Cell Behavior 43 Bahar Bilgen and Sedat Odabas 3.1 Introduction 43 3.2 The Concept of Mechanotransduction 45 3.3 The Mechanical Cues of Cell Differentiation and Tissue Formation on the Basis of Mechanotransduction 46 3.4 Mechanotransduction via External Forces 47 3.4.1 Mechanotransduction via Bioreactors 48 3.4.2 Mechanotransduction via Particle-based Systems 51 3.4.3 Mechanotransduction via Other External Forces 53 3.5 Mechanotransduction via Bioinspired Materials 54 3.6 Future Remarks and Conclusion 54 Declaration of Interest 55 References 55 4 Modulation of Stem Cells Behavior Through Bioactive Surfaces 65 Eduardo D. Gomes, Rita C. Assunção-Silva, Nuno Sousax, Nuno A. Silva and António J. Salgado 4.1 Lithography 66 4.2 Micro and Nanopatterning 70 4.3 Microfluidics 71 4.4 Electrospinning 71 4.5 Bottom-up/Top-down Approaches 74 4.6 Substrates Chemical Modifications 75 4.6.1 Biomolecules Coatings 76 4.6.2 Peptide Grafting 77 4.7 Conclusion 78 References 79 Contents vii 5 Influence of Controlled Micro- and Nanoengineered Environments on Stem Cell Fate 85 Anna Lagunas, David Caballero and Josep Samitier 5.1 Introduction to Engineered Environments for the Control of Stem Cell Differentiation 86 5.1.1 Stem Cells Niche In Vivo: A Highly Dynamic and Complex Environment 86 5.1.2 Mimicking the Stem Cells Niche In Vitro: Engineered Biomaterials 88 5.2 Mechanoregulation of Stem Cell Fate 89 5.2.1 From In Vivo to In Vitro: Influence of the Mechanical Environment on Stem Cell Fate 89 5.2.2 Regulation of Stem Cell Fate by Surface Roughness 90 5.2.3 Control of Stem Cell Differentiation by Micro- and Nanotopographic Surfaces 92 5.2.4 Physical Gradients for Regulating Stem Cell Fate 96 5.3 Controlled Surface Immobilization of Biochemical Stimuli for Stem Cell Differentiation 100 5.3.1 Micro- and Nanopatterned Surfaces: Effect of Geometrical Constraint and Ligand Presentation at the Nanoscale 100 5.3.2 Biochemical Gradients for Stem Cell Differentiation 107 5.4 Three-dimensional Micro- and Nanoengineered Environments for Stem Cell Differentiation 112 5.4.1 Three-dimensional Mechanoregulation of Stem Cell Fate 113 5.4.2 Three-dimensional Biochemical Patterns for Stem Cell Differentiation 119 5.5 Conclusions and Future Perspectives 122 References 122 6 Recent Advances in Nanostructured Polymeric Surface: Challenges and Frontiers in Stem Cells 141 Ilaria Armentano, Samantha Mattioli, Francesco Morena, Chiara Argentati, Sabata Martino, Luigi Torre and Josè Maria Kenny 6.1 Introduction 142 6.2 Nanostructured Surface 144 6.3 Stem Cell 146 6.4 Stem Cell/Surface Interaction 147 6.5 Microscopic Techniques Used in Estimating Stem Cell/Surface 148 6.5.1 Fluorescence Microscopy 148 6.5.2 Electron Microscopy 149 6.5.3 Atomic Force Microscopy 153 6.5.3.1 Instrument 154 6.5.3.2 Cell Nanomechanical Motion 156 6.5.3.3 Mechanical Properties 156 6.6 Conclusions and Future Perspectives 158 References 158 7 Laser Surface Modification Techniques and Stem Cells Applications 165 Çağrı Kaan Akkan 7.1 Introduction 166 7.2 Fundamental Laser Optics for Surface Structuring 166 7.2.1 Definitive Facts for Laser Surface Structuring 167 7.2.1.1 Absorptivity and Reflectivity of the Laser Beam by the Material Surface 167 7.2.1.2 Effect of the Incoming Laser Light Polarization 168 7.2.1.3 Operation Mode of the Laser 169 7.2.1.4 Beam Quality Factor 170 7.2.1.5 Laser Pulse Energy/Power 171 7.2.2 Ablation by Laser Pulses 172 7.2.2.1 Focusing the Laser Beam 172 7.2.2.2 Ablation Regime 173 7.3 Methods for Laser Surface Structuring 174 7.3.1 Physical Surface Modifications by Lasers 174 7.3.1.1 Direct Structuring 175 7.3.1.2 Beam Shaping Optics 177 7.3.1.3 Direct Laser Interference Patterning 180 7.3.2 Chemical Surface Modification by Lasers 181 7.3.2.1 Pulsed Laser Deposition 181 7.3.2.2 Laser Surface Alloying 184 7.3.2.3 Laser Surface Oxidation and Nitriding 186 7.4 Stem Cells and Laser-Modified Surfaces 187 7.5 Conclusions 191 References 192 8 Plasma Polymer Deposition: A Versatile Tool for Stem Cell Research 197 M. N. Macgregor-Ramiasa and K. Vasilev 8.1 Introduction 197 8.2 The Principle and Physics of Plasma Methods for Surface Modification 199 8.2.1 Plasma Sputtering, Etching an Implantation 200 8.2.2 Plasma Polymer Deposition 201 8.3 Surface Properties Influencing Stem Cell Fate 202 8.3.1 Plasma Methods for Tailored Surface Chemistry 203 8.3.1.1 Oxygen-rich Surfaces 204 8.3.1.2 Nitrogen-rich Surfaces 208 8.3.1.3 Systematic Studies and Copolymers 210 8.3.2 Plasma for Surface Topography 211 8.3.3 Plasma for Surface Stiffness 213 8.3.4 Plasma for Gradient Substrata 215 8.3.5 Plasma and 3D Scaffolds 218 8.4 New Trends and Outlook 219 8.5 Conclusions 219 References 220 9 Three-dimensional Printing Approaches for the Treatment of Critical-sized Bone Defects 231 Sara Salehi, Bilal A. Naved and Warren L. Grayson 9.1 Background 232 9.1.1 Treatment Approaches for Critical-sized Bone Defects 232 9.1.2 History of the Application of 3D Printing to Medicine and Biology 233 9.2 Overview of 3D Printing Technologies 234 9.2.1 Laser-based Technologies 235 9.2.1.1 Stereolithography 235 9.2.1.2 Selective Laser Sintering 236 9.2.1.3 Selective Laser Melting 236 9.2.1.4 Electron Beam Melting 237 9.2.1.5 Two-photon Polymerization 237 9.2.2 Extrusion-based Technologies 238 9.2.2.1 Fused Deposition Modeling 238 9.2.2.2 Material Jetting 238 9.2.3 Ink-based Technologies 239 9.2.3.1 Inkjet 3D Printing 239 9.2.3.2 Aerosol Jet Printing 239 9.3 Surgical Guides and Models for Bone Reconstruction 240 9.3.1 Laser-based Surgical Guides 240 9.3.2 Extrusion-based Surgical Guides 240 9.3.3 Ink-based Surgical Guides 241 9.4 Three-dimensionally Printed Implants for Bone Substitution 242 9.4.1 Laser-based Technologies for Metallic Bone Implants 244 9.4.2 Extrusion-based Technologies for Bone Implants 245 9.4.3 Ink-based Technologies for Bone Implants 246 9.5 Scaffolds for Bone Regeneration 246 9.5.1 Laser-based Printing for Regenerative Scaffolds 247 9.5.2 Extrusion-based Printing for Regenerative Scaffolds 247 9.5.3 Ink-based Printing for Regenerative Scaffolds 249 9.5.4 Pre- and Postprocessing Techniques 250 9.5.4.1 Preprocessing 250 9.5.4.2 Postprocessing: Sintering 256 9.5.4.3 Postprocessing: Functionalization 256 9.6 Bioprinting 257 9.7 Conclusion 262 List of Abbreviation 263 References 264 10 Application of Bioreactor Concept and Modeling Techniques to Bone Regeneration and Augmentation Treatments 277 Oscar A. Deccó and Jésica I. Zuchuat 10.1 Bone Tissue Regeneration 278 10.1.1 Proinflammatory Cytokines 279 10.1.2 Transforming Growth Factor Beta 279 10.1.3 Angiogenesis in Regeneration 280 10.2 Actual Therapeutic Strategies and Concepts to Obtain an Optimal Bone Quality and Quantity 281 10.2.1 Guided Bone Regeneration Based on Cells 282 10.2.1.1 Embryonic Stem Cells 282 10.2.1.2 Adult Stem Cells 282 10.2.1.3 Mesenchymal Stem Cells 283 10.2.2 Guided Bone Regeneration Based on PRP and Growth Factors 284 10.2.2.1 Bone Morphogenetic Proteins 287 10.2.3 Guided Bone Regeneration Based on Barrier Membranes 288 10.2.4 Guided Bone Regeneration Based on Scaffolds 290 10.3 Bioreactors Employed for Tissue Engineering in Guided Bone Regeneration 291 10.4 Bioreactor Concept in Guided Bone Regeneration and Tissue Engineering: In Vivo Application 294 10.5 New Multidisciplinary Approaches Intended to Improve and Accelerate the Treatment of Injured and/or Diseased Bone 303 10.5.1 Application of Bioreactor in Dentistry: Therapies for the Treatment of Maxillary Bone Defects 304 10.5.2 Application of Bioreactor in Cases of Osteoporosis 307 10.6 Computational Modeling: An Effective Tool to Predict Bone Ingrowth 310 References 311 11 Stem Cell-based Medicinal Products: Regulatory Perspectives 321 DenizOzdil and Halil Murat Aydin 11.1 Introduction 321 11.2 Defining Stem Cell-based Medicinal Products 323 11.3 Regional Regulatory Issues for Stem Cell Products 326 11.4 Regulatory Systems for Stem Cell-based Technologies 327 11.4.1 The US Regulatory System 328 11.5 Stem Cell Technologies: The European Regulatory System 336 References 340 12 Substrates and Surfaces for Control of Pluripotent Stem Cell Fate and Function 341 Akshaya Srinivasan, Yi-Chin Toh, Xian Jun Loh and Wei Seong Toh 12.1 Introduction 342 12.2 Pluripotent Stem Cells 342 12.3 Substrates for Maintenance of Self-renewal and Pluripotency of PSCs 344 12.3.1 Cellular Substrates 344 12.3.2 Acellular Substrates 345 12.3.2.1 Biological Matrices 345 12.3.2.2 ECM Components 348 12.3.2.3 Decellularized Matrices 350 12.3.2.4 Cell Adhesion Molecules 351 12.3.2.5 Synthetic Substrates 352 12.4 Substrates for Promoting Differentiation of PSCs 355 12.4.1 Cellular Substrates 355 12.4.2 Acellular Substrates 356 12.4.2.1 Biological Matrices 356 12.4.2.2 ECM Components 358 12.4.2.3 Decellularized Matrices 362 12.4.2.4 Cell Adhesion Molecules 363 12.4.2.5 Synthetic Substrates 363 12.5 Conclusions 366 Acknowledgments 367 References 367 13 Silk as a Natural Biopolymer for Tissue Engineering 379 Ayşe Ak Can and Gamze Bölükbaşi Ateş 13.1 Introduction 380 13.2 SF as a Biomaterial 383 13.2.1 Fibroin Hydrogels and Sponges 384 13.2.2 Fibroin Films and Membranes 386 13.2.3 Nonwoven and Woven Silk Scaffolds 386 13.2.4 Silk Fibroin as a Bioactive Molecule Delivery 386 13.3 Biomedical Applications of Silk-based Biomaterials 387 13.3.1 Bone Tissue Engineering 387 13.3.2 Cartilage Tissue Engineering 389 13.3.3 Ligament and Tendon Tissue Engineering 391 13.3.4 Cardiovascular Tissue Engineering 391 13.3.5 Skin Tissue Engineering 393 13.3.6 Other Applications of Silk Fibroin 393 13.4 Conclusion and Future Directions 393 References 394 14 Applications of Biopolymer-based, Surface-modified Devices in Transplant Medicine and Tissue Engineering 399 Ashim Malhotra, Gulnaz Javan and Shivani Soni 14.1 Introduction to Cardiovascular Disease 400 14.2 Need Assessment for Biopolymer-based Devices in Cardiovascular Therapeutics 400 14.3 Emergence of Surface Modification Applications in Cardiovascular Sciences: A Historical Perspective 401 14.4 Nitric Oxide Producing Biosurface Modification 403 14.5 Surface Modification by Extracellular Matrix Protein Adherence 404 14.6 The Role of Surface Modification in the Construction of Cardiac Prostheses 405 14.7 Biopolymer-based Surface Modification of Materials Used in Bone Reconstruction 406 14.8 The Use of Biopolymers in Nanotechnology 409 14.8.1 Protein Nanoparticles 410 14.8.1.1 Albumin-based Nanoparticles and Surface Modification 411 14.8.1.2 Collagen-based Nanoparticles and Surface Modification 412 14.8.1.3 Gelatin-based Nanoparticle Systems 413 14.8.2 Polysaccharide-based Nanoparticle Systems 413 14.8.2.1 The Use of Alginate for Surface Modifications 413 14.8.2.2 The Use of Chitosan-based Nanoparticles and Chitosan-based Surface Modification 414 14.8.2.3 The Use of Chitin-based Nanoparticles and Chitin-based Surface Modification 416 14.8.2.4 The Use of Cellulose-based Nanoparticles and Cellulose-based Surface Modification 417 References 418 15 Stem Cell Behavior on Microenvironment Mimicked Surfaces 423 M. Özgen Öztürk Öncel and Bora Garipcan 15.1 Introduction 424 15.2 Stem Cells 425 15.2.1 Definition and Types 425 15.2.1.1 Embryonic Stem Cells 426 15.2.1.2 Adult Stem Cells 426 15.2.1.3 Reprogramming and Induced Pluripotent Stem Cells 427 15.2.2 Stem Cell Niche 427 15.3 Stem Cells: Microenvironment Interactions 428 15.3.1 Extracellular Matrix 429 15.3.2 Signaling Factors 429 15.3.3 Physicochemical Composition 430 15.3.4 Mechanical Properties 430 15.3.5 Cell–Cell Interactions 431 15.4 Biomaterials as Stem Cell Microenvironments 431 15.4.1 Surface Chemistry 431 15.4.2 Surface Hydrophilicity and Hydrophobicity 434 15.4.3 Substrate Stiffness 435 15.4.4 Surface Topography 435 15.5 Biomimicked and Bioinspired Approaches 436 15.5.1 Bone Tissue Regeneration 439 15.5.2 Cartilage Tissue Regeneration 440 15.5.3 Cardiac Tissue Regeneration 441 15.6 Conclusion 442 References 442
£176.36
John Wiley and Sons Ltd Cellular Physiology of Nerve and Muscle
Book SynopsisCellular Physiology of Nerve and Muscle, Fourth Edition offers a state of the art introduction to the basic physical, electrical and chemical principles central to the function of nerve and muscle cells. The text begins with an overview of the origin of electrical membrane potential, then clearly illustrates the cellular physiology of nerve cells and muscle cells. Throughout, this new edition simplifies difficult concepts with accessible models and straightforward descriptions of experimental results. An all-new introduction to electrical signaling in the nervous system. Expanded coverage of synaptic transmission and synaptic plasticity. A quantitative overview of the electrical properties of cells. New detailed illustrations. Table of ContentsPart I: Origin of Electrical Membrane Potential. 1. Introduction to Electrical Signaling in the Nervous System. The Patellar Reflex as a Model for Neural Function. The Cellular Organization of Neurons. Electrical Signals in Neurons. Transmission between Neurons. 2. Composition of Intracellular and Extracellular Fluids. Intracellular and Extracellular Fluids. The Structure of the Plasma Membrane. Summary. 3. Maintenance of Cell Volume. Molarity, Molality, and Diffusion of Water. Osmotic Balance and Cell Volume. Answers to the Problem of Osmotic Balance. Tonicity. Time-Course of Volume Changes. Summary. 4. Membrane Potential: Ionic Equilibrium. Diffusion Potential. Equilibrium Potential. The Nernst Equation. The Principle of Electrical Neutrality. The Cell Membrane as an Electrical Capacitor. Incorporating Osmotic Balance. Donnan Equilibrium. A Model Cell That Looks Like a Real Animal Cell. The Sodium Pump. Summary. 5. Membrane Potential: Ionic Steady State. Equilibrium Potentials for Sodium, Potassium, and Chloride. Ion Channels in the Plasma Membrane. Membrane Potential and Ionic Permeability. The Goldman Equation. Ionic Steady State. The Chloride Pump. Electrical Current and the Movement of Ions Across Membranes. Factors Affecting Ion Current Across a Cell Membrane. Membrane Permeability vs. Membrane Conductance. Behavior of Single Ion Channels. Summary. Part II: Cellular Physiology of Nerve Cells. 6. Generation of Nerve Action Potential. The Action Potential. Ionic Permeability and Membrane Potential. Measuring the Long-Distance Signal in Neurons. Characteristics of the Action Potential. Initiation and Propagation of Action Potentials. Changes in Relative Sodium Permeability During an Action Potential. Voltage-Dependent Sodium Channels of the Neuron Membrane. Repolarization. The Refractory Period. Propagation of an Action Potential Along a Nerve Fiber. Factors Affecting the Speed of Action Potential Propagation. Molecular Properties of the Voltage-Sensitive Sodium Channel. Molecular Properties of Voltage-Dependent Potassium Channels. Calcium-Dependent Action Potentials. Summary. 7. The Action Potential: Voltage Clamp Experiments. The Voltage Clamp. Measuring Changes in Membrane Ionic Conductance Using the Voltage Clamp. The Squid Giant Axon. Ionic Currents Across an Axon Membrane Under Voltage Clamp. The Gated Ion Channel Model. Membrane Potential and Peak Ionic Conductance. Kinetics of the Change in Ionic Conductance Following a Step Depolarization. Sodium Inactivation. The Temporal Behavior of Sodium and Potassium Conductance. Gating Currents. Summary. 8. Synaptic Transmission at the Neuromuscular Junction. Chemical and Electrical Synapses. The Neuromuscular Junction as a Model Chemical Synapse. Transmission at a Chemical Synapse. Presynaptic Action Potential and Acetylcholine Release. Effect of ACh on the Muscle Cell. Neurotransmitter Release. The Vesicle Hypothesis of Quantal Transmitter Release. Mechanism of Vesicle Fusion. Recycling of Vesicle Membrane. Inactivation of Released Acetylcholine. Recording the Electrical Current Flowing Through a Single Acetylcholine-Activated Ion Channel. Molecular Properties of the Acetylcholine-Activated Channel. Summary. 9. Synaptic Transmission in the Central Nervous System. Excitatory and Inhibitory Synapses. Excitatory Synaptic Transmission Between Neurons. Temporal and Spatial Summation of Synaptic Potentials. Some Possible Excitatory Neurotransmitters. Conductance-Decrease E.P.S.P.'s. Inhibitory Synaptic Transmission. The Synapse Between Sensory Neurons and Antagonist Neurons in the Patellar Reflex. Characteristics of Inhibitory Synaptic Transmission. Mechanism of Inhibition in the Postsynaptic Membrane. Some Possible Inhibitory Neurotransmitters. The Family of Neurotransmitter-Gated Ion Channels. Neuronal Integration. Indirect Actions of Neurotransmitters. Presynaptic Inhibition and Factilitation. Synaptic Plasticity. Short-Term Changes in Synaptic Strength. Long-Term Changes in Synaptic Strength. Summary. Part III: Cellular Physiology of Muscle Cells. 10. Excitation-Contraction Coupling in Skeletal Muscle. The Three Types of Muscle. Structure of Skeletal Muscle. Changes in Striation Pattern on Contraction. Molecular Composition of Filaments. Interaction Between Myosin and Actin. Regulation of Contraction. The Sarcoplasmic Reticulum. The Transverse Tubule System. Summary. 11. Neural Control of Muscle Contraction. The Motor Unit. The Mechanics of Contraction. The Relationship Between Isometric Tension and Muscle Length. Control of Muscle Tension by the Nervous System. Recruitment of Motor Neurons. Fast and Slow Muscle Fibers. Temporal Summation of Contractions Within a Single Motor Unit. Asynchronous Activation of Motor Units During Maintained Contraction. Summary. 12. Cardiac Muscle: The Autonomic Nervous System. Autonomic Control of the Heart. The Pattern of Cardiac Contraction. Coordination of Contraction Across Cardiac Muscle Fibers. Generation of Rhythmic Contractions. The Cardiac Action Potential. The Pacemaker Potential. Actions of Acetylcholine and Norepinephrine on Cardiac Muscle Cells. Summary. Appendix A: Derivation of the Nernst Equation. Appendix B: Derivation of the Goldman Equation. Appendix C: Electrical Properties of Cells. Suggested Readings
£62.65
John Wiley and Sons Ltd Annual Plant Reviews Membrane Transport in Plants
Book SynopsisAnnual Plant Reviews, Volume 15 This volume addresses some of the most important and hotly-pursued topics in the field of plant membrane transport. The first two chapters consider membrane transport analysis, emphasizing concepts, techniques and tools for electrophysiology. Chapters 3-8 divide along boundaries of pumps, coupled transporters and channels; the addition of a chapter on water channels highlights this rapidly expanding and, until recently, highly controversial topic. Chapters 9 and 10 deal with issues of Ca2+ and H+ signalling, and of membrane traffic that increasingly attracts the attention of researchers in plant development. Finally, chapters 11 and 12 take a post-genomic look at the problems of understanding the integration of transport mechanisms and its relevance to inorganic nutrition and phytoremediation. An overriding theme throughout is the extent to which the research on membrane transport now informs the fields of plaTable of Contents1. Concepts and techniques in plant membrane physiology. Michael R. Blatt, Laboratory of Plant Physiology and Biophysics, University of Glasgow, UK. 2. Electrophysiology equipment and software. Adrian Hills and Vadim Volkov, Laboratory of Plant Physiology and Biophysics, University of Glasgow, UK. 3. Structure, function and regulation of primary H+ and Ca2+ pumps. Rosa L. López-Marqués, Morten Schiøtt, Mia Kyed Jakobsen and Michael G. Palmgren, Department of Plant Biology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark. 4. Ion-coupled transport of inorganic solutes. Malcolm J. Hawkesford and Anthony J. Miller, Crop Performance and Improvement Division, Rothamsted Research, Harpenden, UK. 5. Functional analysis of proton-coupled sucrose transport. Daniel R. Bush, USDA-ARS and Plant Biology, University of Illinois, Urbana, USA. 6. Voltage-gated ion channels. Ingo Dreyer, Bernd Müller-Röber and Barbara Köhler, Universität Potsdam, Institut für Biochemie und Biologie, Golm, Germany. 7. Ligand-gated ion channels. Frans Maathius, Biology Department, University of York, UK. 8. Aquaporins in plants. Clare Vander Willigen, Lionel Verdoucq, Yann Boursiac and Christophe Maurel, Department of Molecular and Cellular Biology, University of Cape Town, South Africa. 9. Ca2+ and pH as integrating signals in transport control. Tatiana N. Bibikova, Sarah M. Assmann and Simon Gilroy, Biology Department, Penn. State University, Pennsylvania, USA. 10. Vesicle traffic and plasma membrane transport. Annette C Hurst, Gerhard Thiel and Ulrike Homann, Botanisches Institut, TU-Darmstadt, Darmstadt, Germany. 11. Potassium nutrition and salt stress. Anna Amtmann, Patrick Armengaud and Vadim Volkov, Laboratory of Plant Physiology and Biophysics, University of Glasgow, UK. 12. Membrane transport and soil bioremediation. Susan Rosser and Peter Dominy, Plant Sciences, IBLS, University of Glasgow, UK. References . Index
£242.06
John Wiley and Sons Ltd Annual Plant Reviews Intercellular Communication
Book SynopsisAnnual Plant Reviews, Volume 16 Intercellular communication in plants plays a vital role in the co-ordination of processes leading to the formation of a functional organism.Table of Contents1. Auxin as an intercellular signal. Jiri Friml and Justyna Wisniewska, Department of Developmental Genetics, University of Tübingen, Germany. 2. Peptides as signals. Yiji Xia, Donald Danforth Plant Science Center, St Louis, Missouri, USA. 3. RNA as a signalling molecule. Patrice Dunoyer and Olivier Voinnet, Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France. 4. The plant extracellular matrix and signalling. Andrew Fleming, Department of Animal and Plant Sciences, University of Sheffield, UK. 5. Plasmodesmata – gateways for intercellular communication in plants. Trudie Gillespie and Karl Oparka, Scottish Crop Research Institute, Dundee, UK. 6. Lessons from the vegetative shoot apex. John Golz, School of Biological Sciences, University of Victoria, Australia. 7. Intercellular communication during floral initiation and development. George Coupland, Max Planck Institute for Plant Breeding, Cologne, Germany. 8. Lessons from the root apex. Martin Bonke, Sari Tähtiharju and Ykä Helariutta, Institute of Biotechnology, University of Helsinki, Finland. 9. Lessons from leaf epidermal patterning in plants. Bhylahalli Purushottam and Martin Hülskamp, Botanical Institute, University of Cologne, Germany. 10. Lessons on signalling in plant self-incompatibility systems. Andrew G. McCubbin, School of Biological Sciences, Washington State University, Pullmann, USA. References. Index
£218.66
John Wiley and Sons Ltd Annual Plant Reviews Plasmodesmata
Book SynopsisAnnual Plant Reviews, Volume 18 Since their discovery over 100 years ago, plasmodesmata have been the focus of intense investigation. Plasmodesmata are unique to plants and form an intercellular continuum for the transport of solutes, signals and ribonucleoprotein complexes.Table of ContentsContributors. Preface. 1. Plasmodesmal structure and development. Alison G. Roberts, Scottish Crop Research Institute, Dundee, UK. 2. Evolution of plasmodesmata. John A. Raven, Division of Environmental and Applied Biology, University of Dundee, UK. 3. Plasmodesmata: protein transport signals and receptors. Friedrich Kragler, Vienna Biocenter, Austria. 4. Comparative structures of specialised monocotyledonous leaf blade plasmodesmata. C. E. J. Botha, Botany Department, Rhodes University, Grahamstown, South Africa, R. H. M. Cross, Electron Microscopy Unit, Rhodes University, Grahamstown, South Africa and L. Liu, Linyi University, Linyi, China. 5. Plasmodesmata and plant morphogenesis. Ken Kobayashi, Insoon Kim, Euna Cho and Patricia Zambryski, Department of Plant and Microbial Biology, University of California, Berkeley, USA. 6. Transcription factor movement through plasmodesmata. David Jackson, Cold Spring Harbor Laboratory, New York, USA. 7. Role of plasmodesmata in solute loading and unloading. Alexander Schulz, Department of Plant Biology, The Royal Veterinary and Agricultural University, Copenhagen, Denmark. 8. Plasmodesmata and the phloem: conduits for local and long-distance signaling. Robert L. Gilbertson, Maria R. Rojas, and William J. Lucas, Department of Plant Pathology, University of California, Davis, USA. 9. Movement of viruses to and through plasmodesmata. Richard S. Nelson, Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA. 10. Systemic RNA silencing. Manfred Heinlein, Institute of Botany, University of Basel, Switzerland. 11. Techniques for imaging intercellular transport. Karl Oparka and Petra Boevink, Unit of Cell Biology, Scottish Crop Research Institute, Dundee, UK. 12. Electrical signalling via plasmodesmata. Aart J. E. van Bel and Katrina Ehlers, Institute of General Botany, Justus-Liebig University, Giessen, Germany. 13. Regulation of plasmodesmal conductance. Terena L. Holdaway-Clarke, School of Biological Sciences, University of Sydney, New South Wales, Australia. Index
£205.16
John Wiley and Sons Ltd Annual Plant Reviews Cell Cycle Control and Plant
Book SynopsisThe cell cycle in plants consists of an ordered set of events, including DNA replication and mitosis, that culminates in cell division. As cell division is a fundamental part of a plant's existence and the basis for tissue repair, development and growth, a full understanding of all aspects of this process is of pivotal importance. Cell Cycle Control and Plant Development commences with an introductory chapter and is broadly divided into two parts. Part 1 details the basic cell machinery, with chapters covering cyclin-dependent kinases (CDKs), cyclins, CDK inhibitors, proteolysis, CDK phosphorylation, and E2F/DP transcription factors. Part 2, which describes the cell cycle and plant development, covers cell cycle activation, cell cycle control during leaf development, endoreduplication, the cell cycle and trichome, fruit and endosperm development, the hormonal control of cell division and environmental stress, and cell cycle exit. The editor of this important bookTrade Review"Excellent new volume ... .An essential purchase for research teams ... .All libraries ... should have copies of this essential and timely volume." Biotechnology, Agronomy, Society and Environment “Welcome and timely ... .Much of the information was relatively new to me; an enjoyable read … .Full of excellent information, carefully and thoughtfully presented.” Annals of BotanyTable of Contents1. The growing family of plant cyclin-dependent kinases with multiple functions in cellular and developmental regulation. 2. The plant cyclins. 3. CDK inhibitors. 4. The UPS: an engine that drives the cell cycle. 5. CDK phosphorylation. 6. E2F-DP transcription factors. 7. Function of the retinoblastoma-related protein in plants. 8. Auxin fuels the cell cycle engine during lateral root initiation. 9. Cell cycle control during leaf development. 10. Physiological relevance and molecular control of the endocycle in plants. 11. Insights into the endocycle from trichome development. 12. Cell cycle control and fruit development. 13. Cell cycle and endosperm development. 14. Hormonal regulation of cell cycle progression and its role in development. 15. Cell cycle and environmental stresses
£172.76
University of Toronto Press Dreams and Due Diligence
Book SynopsisDreams and Due Diligence vividly chronicles the work of two researchers who made medical history two men who possessed exactly the right complementary talents to achieve greatness and win nearly every award available in medical research.Table of ContentsContents Introduction Part I: Discovery 1. On a Sunday in 1960 2. After the A-Bomb, before the Beatles 3. The Impossible partnership Part II: Development 4. A bunch of kids having a good time 5. The progeny Part III: Today and Tomorrow 6. Ethics, hope and hype 7. The evil twin: the cancer stem cell 8. The beneficiary 9. The future 10. Little fame, no Nobel Conclusion
£26.99
Humana Press Inc. Organoids
Book SynopsisThis detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research.Table of Contents1. A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment Magdalena Sutcliffe and Madeline A. Lancaster 2. Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells Eva Tomaskovic-Crook and Jeremy M. Crook 3. Organoid Assay of Human Cancer Stem Cells Yohei Shimono, Piero Dalerba, Junko Mukohyama, Taichi Isobe, Darius Johnston, and Akira Suzuki 4. Construction of Thymus Organoids from Decellularized Thymus Scaffolds Asako Tajima, Isha Pradhan, Xuehui Geng, Massimo Trucco, and Yong Fan 5. Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres Robert E. Hynds, Colin R. Butler, Sam M. Janes, and Adam Giangreco 6. Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells Mahboobe Ghaedi and Laura E. Niklason 7. Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix Hiroko Hisha and Hiroo Ueno 8. Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension Valentina Benedetti, Valerio Brizi, and Christodoulos Xinaris 9. Efficient Culture of Intestinal Organoids with Blebbistatin Zhen Qi and Ye-Guang Chen 10. Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion Dustin J. Flanagan, Renate H.M. Schwab, Bang M. Tran, Toby J. Phesse, and Elizabeth Vincan 11. The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, and Maja Grabacka 12. New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice Toshio Takahashi 13. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System Jessica L. Forbester, Nicholas Hannan, Ludovic Vallier, and Gordon Dougan 14. Murine Colonic Organoid Culture System and Downstream Assay Applications Yang-Yi Fan, Laurie A. Davidson, and Robert S. Chapkin 15. Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions Giulia Nigro, Melissa Hanson, Cindy Fevre, Marc Lecuit, and Philippe J. Sansonetti 16. The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism Diana Li, Hongli Dong, and Alison B. Kohan 17. Oncogenic Transformation of Human-Derived Gastric Organoids Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, and Yana Zavros 18. Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation Yun Chen, Chuan Li, Sheng-Hong Tseng, and Ya-Hui Tsai 19. Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections Winnie Y. Zou, Sarah E. Blutt, Sue E. Crawford, Khalil Ettayebi, Xi-Lei Zeng, Kapil Saxena, Sasirekha Ramani, Umesh Karandikar, Nicholas C. Zachos, and Mary K. Estes 20. Study Bacterial-Host Interactions Using Intestinal Organoids Yong-guo Zhang and Jun Sun 21. Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering Megan K. Eldred, Leila Muresan, and William A. Harris 22. Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo Kai Tong, Mahendra Wagle, and Su Guo 23. Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells Yoni Baert, Charlotte Rombaut, and Ellen Goossens 24. Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids Tiziana A.L. Brevini, Elena F.M. Manzoni, Sergio Ledda, and Fulvio Gandolfi 25. Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly Yuqing Zhu, Vahid Serpooshan, Sean Wu, Utkan Demirci, Pu Chen, and Sinan Güven 26. Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying Mozhdeh Imaninezhad, Era Jain, and Silviya Petrova Zustiak 27. Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues Soojung Hahn and Jongman Yoo 28. Drug Sensitivity Assays of Human Cancer Organoid Cultures Hayley E. Francies, Andrew Barthorpe, Anne McLaren-Douglas, William J. Barendt, and Mathew J. Garnett
£89.99
American Society for Microbiology The Bacterial Spore: From Molecules to Systems
Book Synopsis
£86.36
John Wiley and Sons Ltd Barriers and Channels Formed by Tight Junction
Book SynopsisTight junction proteins connect epithelial and endothelial cells and, importantly, form selective barriers and channels for paracellular transportation. Two types of tight junction proteins—the claudin family (claudins-1 to -27) and the TAMP family (occludin, Marvel-D2, and tricellulin)—can be distinguished molecularly and functionally. Recent work has focused on delineating the functional significance of differences in selective permeability, for example, by examining the regulatory mechanisms of tight junctions. This work is of great clinical importance as many inflammatory or infectious diseases are characterized by increased permeability and the manipulation of tight junctions presents a new horizon in the development of novel therapeutics. The volume Barriers and Channels Formed by Tight Junction Proteins II provides a detailed look at the recent advances in our understanding of tight junction regulation and dysregulation. The papers included focus on three major themes: inflammation and gastrointestinal function; epithelial transport, barrier modulation, and food components; and tight junctions in intestinal and renal epithelia. NOTE: Annals volumes are available for sale as individual books or as a journal. For more information on instutional journal subscriptions, please visit http://ordering.onlinelibrary.wiley.com/subs.asp?ref=1749-6632&doi=10.111/(ISSN)1749-6632 ACADEMY MEMBERS: Please contact the New York Academy of Sciences directly to place your order (www.nyas.org). Members of the New York Academy of Science receive full-text access to Annals online and discounts on print volumes. Please visit http://www.nyas.org/MemberCenter/Join.aspx for more information on becoming a member.
£57.56
