Description
Book SynopsisThis fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshoo
Table of ContentsPart I: Overview Articles
1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development
Philip Kirk and Yoselin Benitez-Alfonso
2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses
Caiping Huang and Manfred Heinlein
Part II: Structure and Distribution of Plasmodesmata
3. Plasmodesmata Ultrastructure Determination Using Electron Tomography
Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer
4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata
Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska
5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots
Andrea Paterlini and Ilya Belevich
6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities
Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith
7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy
Florence R. Danila
8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy
Kirsten Knox
Part III: Compositional Analysis of Plasmodesmata
9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata
Caiping Huang, Jerôme Mutterer, and Manfred Heinlein
10. Immunofluorescence Detection of Callose in Plant Tissue Sections
Sam Amsbury and Yoselin Benitez-Alfonso
11. Callose Detection and Quantification at Plasmodesmata in Bryophytes
Arthur Muller, Tomomichi Fujita, and Yoan Coudert
12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis
Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison
13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons
Verena Kriechbaumer and Stanley W. Botchway
14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer Patrick Blümke, Vicky Howe, and Rüdiger Simon
15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy
Joseph F. McKenna
16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering
Ajeet Chaudhary and Kay Schneitz
Part IV: Functional Analysis of Plasmodesmata
17. Quantification of Cell-to-Cell Connectivity Using Particle Bombardment
Estee E. Tee, Sebastian Samwald, and Christine Faulkner
18. Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay
Jessica C. Fernandez and Tessa M. Burch-Smith
19. Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay
Wilson Horner and Jacob O. Brunkard
20. An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins
Frank Machin, Yağmur Hasbioğlu, and Friedrich Kragler
21. Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP
Min Diao and Shanjin Huang
22. Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes
Takumi Tomoi, Yoan Coudert, and Tomomichi Fujita
23. Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability
Ekaterina A. Lazareva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergey Y. Morozov, Manfred Heinlein, and Andrey G. Solovyev
24. Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis
Justyna Wróbel-Marek, Kamila Godel-Jędrychowska, and Ewa Kurczyńska
25. Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling
Lisa Van den Broeck, Mariah Gobble, and Rosangela Sozzani
26. Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction
Dawei Yan
27. A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue
Munenori Kitagawa and David Jackson
Part V: Other Approaches Contributing to Plasmodesmata Research
28. In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG
Eduardo J. Peña and Manfred Heinlein
29. Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM)
Michael Fuchs and Jan U. Lohmann
30. More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight
Eva E. Deinum
31. Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis
Gwendolyn V. Davis, Richard S. Smith, and George W. Bassel
