Biochemical engineering Books

Book SynopsisChemical reaction engineering is concerned with the exploitation of chemical reactions on a commercial scale. Ita s goal is the successful design and operation of chemical reactors. This text emphasizes qualitative arguments, simple design methods, graphical procedures, and frequent comparison of capabilities of the major reactor types.Table of ContentsPartial table of contents: Overview of Chemical Reaction Engineering. HOMOGENEOUS REACTIONS IN IDEAL REACTORS. Introduction to Reactor Design. Design for Single Reactions. Design for Parallel Reactions. Potpourri of Multiple Reactions. NON IDEAL FLOW. Compartment Models. The Dispersion Model. The Tank-in-Series Model. REACTIONS CATALYZED BY SOLIDS. Solid Catalyzed Reactions. The Packed Bed Catalytic Reactor. Deactivating Catalysts. HETEROGENEOUS REACTIONS. Fluid-Fluid Reactions: Kinetics. Fluid-Particle Reactions: Design. BIOCHEMICAL REACTIONS. Enzyme Fermentation. Substrate Limiting Microbial Fermentation. Product Limiting Microbial Fermentation. Appendix. Index.

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Book SynopsisAbout our authors Christie John Geankoplis was a professor of chemical engineering and materials science at the University of Minnesota. His research interests involved transport processes, biochemical reactor engineering, mass transfer in liquid solutions, and diffusion and/or reaction in porous solids. Allen Hersel is an associate professor in the Department of Chemical Engineering at Trine University in Angola, Indiana, where he teaches transport phenomena and separations for the last 19 years. He also served as the dean of the engineering school. His area of research is bio-separations and engineering education. Before entering academia, he worked for Koch Industries and Kellogg Brown & Root. He holds a Ph.D. in chemical engineering from Yale University. Daniel H. Lepek is a professor at the Department of Chemical Engineering at The Cooper Union. His research interests include particle technology, fluidiza

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Book SynopsisHydroquinone has a variety of uses principally associated with its action as a reducing agent which is soluble in water. It is a major component in most photographic developers for film and paper, and is used as a topical application in skin whitening to reduce the colour of skin. In this book, the authors present current research in the production, uses and health effects of hydroquinone. Topics discussed include the cellular effects of hydroquinone; the involvement of cigarette smoke-related hydroquinone in the pathogenesis of age-related macular degeneration; hydroquinone solubility and separation processes; the transport and transfer processes of poly(o-aminophenol)film electrodes in the presence of the hydroquinone/p-benzoquinone redox couple and the detection of thiols using hydroquinone on gold surfaces.

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Book SynopsisThis detailed guide to making templates used in cutting pipes includes: Drawing practice Dividing lines Angles, Circles, and the Use of Irregular Curves Instruction for Making Pipe Templates for 15 different variations of cuts are included.

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Book SynopsisThis edition is suitable as a text for Chemical Process Dynamics or Introductory Chemical Process Control courses at the junior/senior level. Also, for Numerical Methods courses in chemical engineering. The goal of this book is to provide an introduction to the modeling, analysis, and simulation of the dynamic behavior of chemical processes.Table of ContentsI. PROCESS MODELING. 1. Introduction. Motivation. Models. Systems. Background of the Reader. How To Use This Textbook. Courses Where This Textbook Can Be Used. 2. Process Modeling. Background. Balance Equations. Material Balances. Constitutive Relationships. Material and Energy Balances. Distributes Parameter Systems. Dimensionless Models. Explicit Solutions to Dynamic Models. General Form of Dynamic Models. II. NUMERICAL TECHNIQUES. 3. Algebraic Equations. Notations. General Form for a Linear System of Equations. Nonlinear Functions of a Single Variable. MATLAB Routines for Solving Functions of a Single Variable. Multivariable Systems. MATLAB Routines for Systems of Nonlinear Algebraic Equations. 4. Numerical Integration. Background. Euler Integration. Runge-Kutta Integration. MATLAB Integration Routines. III. LINEAR SYSTEMS ANALYSIS. 5. Linearization of Nonlinear Models: The State-Space Formulation. State Space Models. Linearization of Nonlinear Models. Interpretation of Linearization. Solution of the Zero-Input Form. Solution of the General State-Space Form. MATLAB Routines step and initial. 6. Solving Linear nth Order ODE Models. Background. Solving Homogeneous, Linear ODEs with Constant Coefficients. Solving Nonhomogeneous, Linear ODEs with Constant Coefficients. Equations with Time-Varying Parameters. Routh Stability Criterion—Determining Stability Without Calculating Eigenvalues. 7. An Introduction to Laplace Transforms. Motivation. Definition of the Laplace Transform. Examples of Laplace Transforms. Final and Initial Value Theorems. Application Examples. Table of Laplace Transforms. 8. Transfer Function Analysis of First-Order Systems. Perspective. Responses of First-Order Systems. Examples of Self-Regulating Processes. Integrating Processes. Lead-Lag Models. 9. Transfer Function Analysis of Higher-Order Systems. Responses of Second-Order Systems. Second-Order Systems with Numerator Dynamics. The Effect of Pole-Zero Locations on System Step Responses. Pad Approximation for Deadtime. Converting the Transfer Function Model to State-Space Form. MATLAB Routines for Step and Impulse Response. 10. Matrix Transfer Functions. A Second-Order Example. The General Method. MATLAB Routine ss2tf. 11. Block Diagrams. Introduction to Block Diagrams. Block Diagrams of Systems in Series. Pole-Zero Cancellation. Systems in Series. Blocks in Parallel. Feedback and Recycle Systems. Routh Stability Criterion Applied to Transfer Functions. SIMULINK. 12. Linear Systems Summary. Background. Linear Boundary Value Problems. Review of Methods for Linear Initial Value Problems. Introduction to Discrete-Time Models. Parameter Estimation of Discrete Linear Systems. IV. NONLINEAR SYSTEMS ANALYSIS. 13. Phase-Plane Analysis. Background. Linear System Examples. Generalization of Phase-Plane Behavior. Nonlinear Systems. 14. Introduction Nonlinear Dynamics: A Case Study of the Quadratic Map. Background. A Simple Population Growth Model. A More Realistic Population Model. Cobweb Diagrams. Bifurcation and Orbit Diagrams. Stability of Fixed-Point Solutions. Cascade of Period-Doublings. Further Comments on Chaotic Behavior. 15. Bifurcation Behavior of Single ODE Systems. Motivation. Illustration of Bifurcation Behavior. Types of Bifurcations. 16. Bifurcation Behavior of Two-State Systems. Background. Single-Dimensional Bifurcations in the Phase-Plane. Limit Cycle Behavior. The Hopf Bifurcation. 17. Introduction to Chaos: The Lorenz Equations. Introduction. Background. The Lorenz Equations. Stability Analysis of the Lorenz Equations. Numerical Study of the Lorenz Equations. Chaos in Chemical Systems. Other Issues in Chaos. IV. REVIEW AND LEARNING MODULES. Module 1 Introduction to MATLAB. Module 2 Review of Matrix Algebra. Module 3 Linear Regression. Module 4 Introduction to SIMULINK. Module 5 Stirred Tank Heaters. Module 6 Absorption. Module 7 Isothermal Continuous Stirred Tank Chemical Reactors. Module 8 Biochemical Reactors. Module 9 Diabatic Continuous Stirred Tank Reactors. Module 10 Ideal Binary Distillation. Index.

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Book SynopsisThis concise text contains the essential material covered in much longer texts, making it easier for students to understand the key principles. There are chapters on conduction, forced convection, natural convection and radiation. These are integrated in examples which need more than one aspect of heat transfer for solution.Table of ContentsNomenclature ; 1. Introduction ; 2. Conduction ; 3. Forced Convection ; 4. Natural Convection ; 5. Radiation ; Appendix - Properties of air and water. Error function

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Book SynopsisFormation of products by paste flow and extrusion occurs in a multitude of industries including the manufacture of a broad range of ceramics, foods and pharmaceuticals. This unique text is the first to demonstrate, to suppliers and users of paste flow equipment, a scientific means of approaching design and operation. It will be indispensable in developing new products and processes in addition to enhancing existing ones. All types of equipment are considered and particular emphasis is given to paste characterization and die design. Based on 25 years'' indusrial experience and research work, the text blends theory and practice, with emphasis on the practical applications, for anyone with a background in engineering or science.Trade ReviewThis is an attractive book that is easy to read ... They have captured both the practice and the principles in a way that will lead a newcomer past the usual barriers to entry, and will refresh the experienced with a broader perspective ... This book brings a rational and simplifying approach, and will be welcomed by teachers and practitioners alike. The Chemical Engineering Journal, 55 (1994) 97 - 100Table of ContentsIntroduction ; 1. Paste extrusion and other processes ; 2. Types of extruder ; 3. Fundamentals of paste flow ; 4. Laboratory evaluation methods ; 5. Paste formulation ; 6. Flow defects and phase migration ; 7. Die design and construction ; 8. Screw extruders ; 9. An overview ; 10. Worked example

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Book SynopsisThe study of dielectric properties of biological systems and their components is important not only for fundamental scientific knowledge but also for its applications in medicine, biology, and biotechnology. The associated technique - known as dielectric spectroscopy - has enabled researchers to quickly and accurately acquire time- or frequency-spectra of permittivity and conductivity and permitted the derivation and testing of realistic electrical models for cells and organelles. This text covers the theoretical basis and practical aspects of the study of dielectric properties of biological systems, such as water, electrolyte and polyelectrolytes, solutions of biological macromolecules, cells suspensions and cellular systems. The authors'' combined efforts provide a comprehensive and cohesive book that takes advantage of the expertise of multiple scientists involved in cutting-edge research in the specific sub-fields of bio-dielectric spectroscopy while maintaining its self-consistencTrade ReviewThis book is a tremendously useful tool for researchers who wish to understand dielectric relaxation methods aimed at studying biological materials. It covers experimental techniques, data analyses, as well as models, in each case ranging from historical backgrounds to state-of-the-art approaches. Challenging features of biological materials such as interfacial dielectric effects and the role of water are covered in detail by experts in the field. The book provides many examples of dielectric signatures of biological processes and illustrates these by informative figures. * Ranko Richert, *The textbook presents a view of dielectric theory, analysis and associated experimental techniques with a focus on biological applications. It addresses the determination of permittivity over a wide range of frequencies and conductivities and presents the methodology for dealing with electrode polarization by experimental techniques and analysis. The text presents a compendium of data, contemporary analyses and results that delineate the system dynamics on biologically interesting systems, ranging from water to macromolecules to membranes to cells. This text is intended for scientists who need information on such systems and/or who want to augment their research arsenal with dielectric measurements. This text is well annotated with useful references. * John G. Berberian *Table of ContentsPART I: THEORETICAL BACKGROUND; PART II: EXPERIMENTAL METHODS AND TECHNIQUES; PART III: APPLICATIONS

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Book SynopsisAmino Acid Metabolism, 3rd Edition covers all aspects of the biochemistry and nutritional biochemistry of the amino acids. Starting with an overview of nitrogen fixation and the incorporation of inorganic nitrogen into amino acids, the book then details other major nitrogenous compounds in micro-organisms, plants and animals. Contents include a discussion of the catabolism of amino acids and other nitrogenous compounds in animals, and the microbiological reactions involved in release of nitrogen gas back into the atmosphere. Mammalian (mainly human) protein and amino acid requirements are considered in detail, and the methods that are used to determine them. Chapters consider individual amino acids, grouped according to their metabolic origin, and discussing their biosynthesis (in plants and micro-organisms for those that are dietary essentials for human beings), major metabolic roles (mainly in human metabolism) and catabolism (again mainly in human metabolTrade Review“Summing Up: Recommended. Upper-division undergraduates through professionals.” (Choice, 1 March 2013) “Bender writes succinctly and clearly, in a manner which serves well for quick referencing or for reading whole chapters at a time. The chapters are well organised and arranged logically.” (Phenotype, 1 February 2013) Table of ContentsFigures xiii Tables xvii Preface xix 1 Nitrogen Metabolism 1 1.1 Nitrogen fixation 3 1.1.1 Nitrogenase 5 1.1.1.1 The nitrogen fixation gene cluster 7 1.1.1.2 Regulation of nitrogenase by the availability of fixed nitrogen and ATP 7 1.1.1.3 Protection of nitrogenase against oxygen 9 1.1.1.4 Respiratory protection in aerobic microorganisms 9 1.1.1.5 Conformational changes in nitrogenase 10 1.1.1.6 Heterocyst formation in filamentous cyanobacteria 10 1.1.1.7 Symbiotic Rhizobium spp. in root nodules 10 1.2 Nitrification and denitrification 11 1.2.1 The anammox (ANaerobic AMMonium OXidation) reaction 12 1.3 The incorporation of fi xed nitrogen into organic compounds 12 1.3.1 Utilization of nitrite and nitrate in plants 12 1.3.2 Incorporation of ammonium into organic compounds 13 1.3.2.1 Reductive amination – the glutamate pathway of ammonium incorporation 14 1.3.2.2 Glutamate dehydrogenase 16 1.3.2.3 Mammalian glutamate dehydrogenase 17 1.3.2.4 Glutamate synthase – the glutamine pathway of ammonium incorporation 18 1.3.2.5 Synthesis of aspartate and asparagine 21 1.4 The synthesis and catabolism of purine and pyrimidine nucleotides 23 1.4.1 Purine synthesis 26 1.4.1.1 Phosphoribosyl pyrophosphate (PRPP) synthetase 28 1.4.1.2 PRPP amidotransferase 30 1.4.2 Purine catabolism and salvage 31 1.4.2.1 Adenosine deaminase deficiency – severe combined immune deficiency 34 1.4.2.2 Gout and hyperuricaemia 35 1.4.2.3 HGPRT deficiency – the Lesch-Nyhan syndrome 37 1.4.3 Pyrimidine synthesis 38 1.4.3.1 Orotic aciduria 42 1.4.4 Pyrimidine catabolism and salvage 43 1.5 Deamination of amino acids 45 1.5.1 Amino acid oxidases 45 1.5.2 Amine oxidases 47 1.5.3 Glutamate and alanine dehydrogenases 48 1.5.4 Non-oxidative deamination of amino acids 49 1.5.5 Glutaminase and asparaginase 50 1.6 Excretion of nitrogenous waste 51 1.6.1 Uricotelic and purinotelic species 51 1.6.2 Ureotelic species 52 1.6.2.1 Urea synthesis 52 1.6.2.2 Inborn errors of metabolism affecting the urea synthesis cycle 57 1.6.2.3 Entero-hepatic circulation of urea 59 1.6.2.4 Canavanine 60 1.7 Other nitrogenous compounds in human urine 61 1.7.1 Aminoacidurias 62 Further reading 65 2 Nitrogen Balance and Protein Turnover – Protein and Amino Acids in Human Nutrition 67 2.1 Nitrogen balance and protein requirements 67 2.1.1 Protein digestion and absorption 69 2.1.2 Protein digestibility and unavailable amino acids in dietary proteins 74 2.1.3 Obligatory nitrogen losses 75 2.1.4 Dynamic equilibrium and tissue protein turnover 76 2.1.5 Tissue protein catabolism 77 2.1.5.1 Lysosomal autophagy 78 2.1.5.2 Ubiquitin and the proteasome 79 2.1.5.3 Active site proteolysis of apo-enzymes 81 2.1.6 Whole body protein turnover 81 2.1.6.1 The constant infusion, labelled precursor method 82 2.1.6.2 The constant infusion, labelled end product method 82 2.1.6.3 Rates of whole-body protein turnover 83 2.1.6.4 The catabolic drive and amino acid oxidation 83 2.1.6.5 The energy cost of protein turnover 84 2.1.6.6 Diurnal variation in protein turnover 85 2.2 Requirements for individual amino acids 86 2.2.1 Nitrogen balance studies 89 2.2.2 Isotope tracer studies 90 2.2.3 Control of protein synthesis by the availability of amino acids 91 2.2.4 Protein quality (protein nutritional value) 92 2.2.4.1 Biological assays of protein quality 93 2.2.4.2 Chemical analysis and protein quality 94 2.3 The fate of amino acid carbon skeletons and the thermic effect of protein 94 2.4 Inter-organ metabolism of amino acids 99 2.5 Transport of amino acids across membranes 100 2.5.1 Families of amino acid transporters 101 2.5.1.1 Dipeptide transport 104 Further reading 104 3 The Role of Vitamin B6 in Amino Acid Metabolism 105 3.1 Pyridoxal phosphate-dependent reactions 106 3.1.1 Families of pyridoxal phosphate-dependent enzymes 111 3.2 Amino acid racemases 112 3.2.1 Bacterial alanine racemase 112 3.2.2 Eukaryotic serine racemase 113 3.2.3 D-Aspartate in eukaryotes 114 3.2.4 D-Amino acids in aquatic invertebrates 115 3.2.5 D-Amino acids in gene-encoded peptides and proteins 115 3.3 Transamination 117 3.3.1 Dual substrate recognition in transaminases 120 3.3.2 Aspartate transaminase and the malate-aspartate shuttle 120 3.4 Decarboxylation and side-chain elimination and replacement reactions 122 3.4.1 Transamination of decarboxylases and enzymes catalyzing side-chain limination reactions 122 3.5 Pyruvate-containing enzymes 124 3.6 Vitamin B6 defi ciency and dependency 125 Further reading 128 4 Glycine, Serine and the One-Carbon Pool 129 4.1 Sources of glycine 130 4.1.1 Choline as a source of glycine 130 4.1.2 Glycine transaminase 132 4.2 The interconversion of glycine and serine 132 4.2.1 Serine hydroxymethyltransferase 133 4.2.2 The glycine cleavage system 135 4.2.3 Serine hydroxymethyltransferase and the glycine cleavage system in photosynthetic tissue 136 4.2.4 Non-ketotic and ketotic hyperglycinaemia 137 4.3 Glycine oxidase and glyoxylate metabolism 138 4.3.1 Primary hyperoxaluria 140 4.4 One-carbon metabolism 141 4.5 Serine biosynthesis 141 4.6 Serine catabolism 144 4.6.1 Serine transamination 144 4.6.2 Serine deaminase 145 4.7 Peptidyl glycine hydroxylase (peptide α-amidase) 146 4.8 5-Aminolevulinic acid and porphyrin synthesis 147 4.8.1 Porphyrias – diseases of porphyrin synthesis 151 4.9 Selenocysteine 152 Further reading 154 5 Amino Acids Synthesized from Glutamate: Glutamine, Proline, Ornithine, Citrulline and Arginine 157 5.1 Synthesis of 5-aminolevulinic acid from glutamate in plants 159 5.2 The catabolism of glutamate 160 5.3 Glutamine 161 5.3.1 Indirect formation of glutamine-tRNA 163 5.3.2 Glutaminases 164 5.3.2.1 Glutamine-dependent amidotransferases 164 5.3.3 Transglutaminases 165 5.4 Glutathione and the γ-glutamyl cycle 168 5.4.1 Glutathione peroxidases 170 5.4.2 Glutathione reductase 171 5.4.3 Glutathione S-transferases 171 5.4.4 Glutathione synthesis 174 5.4.4.1 Glutamate cysteine ligase 174 5.4.4.2 Glutathione synthetase 175 5.4.5 The γ-glutamyl cycle 176 5.5 Glutamate decarboxylase and the GABA shunt 178 5.5.1 Glutamate decarboxylase 180 5.5.2 Alternative pathways of GABA synthesis 181 5.5.3 GABA catabolism 183 5.6 Glutamate carboxylase and vitamin K-dependent post-synthetic modification of proteins 184 5.6.1 Vitamin K-dependent proteins in blood clotting 187 5.6.2 Osteocalcin and matrix Gla protein 189 5.6.3 Vitamin K-dependent proteins in cell signalling – Gas-6 and protein S 190 5.7 Proline 190 5.7.1 Proline synthesis and catabolism 192 5.7.1.1 Δ1-pyrroline-5-carboxylate reductase and proline oxidase 192 5.7.1.2 Hydroxyproline catabolism 194 5.7.2 Peptide prolyl hydroxylase 196 5.7.2.1 The hypoxia-inducible factor 198 5.8 The polyamines 198 5.8.1 Ornithine decarboxylase 199 5.8.2 S-Adenosylmethionine decarboxylase and polyamine synthesis 201 5.8.3 Polyamine catabolism and the interconversion pathway 203 5.8.4 Hypusine 204 5.9 Arginine, citrulline and ornithine 205 5.9.1 Arginine biosynthesis 206 5.9.1.1 The role of citrulline in arginine biosynthesis in mammals 208 5.9.2 Arginine catabolism in microorganisms 209 5.9.3 Nitric oxide 210 5.9.3.1 Nitric oxide synthase 211 5.9.3.2 Arginase and the control of arginine availability for nitric oxide synthesis or polyamine synthesis 214 5.9.4 Agmatine 216 5.9.5 Post-synthetic methylation of arginine in proteins 217 5.9.6 Post-synthetic formation of citrulline in proteins 218 5.9.7 Creatine 219 Further reading 222 6 Amino Acids Synthesized from Aspartate: Lysine, Methionine (and Cysteine), Threonine and Isoleucine 225 6.1 Regulation of the pathway of amino acid synthesis from aspartate 227 6.1.1 Aspartate kinase 228 6.1.1.1 Aspartate kinase in post-synthetic modification of proteins 230 6.1.1.2 Aspartic semialdehyde dehydrogenase 230 6.1.2 Homoserine dehydrogenase 230 6.1.3 Homoserine kinase 231 6.1.4 Threonine synthase 232 6.1.5 Threonine catabolism 232 6.1.5.1 Threonine deaminase 234 6.2 Lysine 235 6.2.1 Lysine biosynthesis in bacteria and plants – the diaminopimelate pathway 236 6.2.1.1 Diaminopimelate and dipicolinate in sporulating bacteria 238 6.2.2 Lysine biosynthesis in yeasts and fungi – the α-amino adipic acid pathway 239 6.2.3 Lysine catabolism 242 6.2.3.1 The saccharopine pathway of lysine catabolism 243 6.2.3.2 The pipecolic acid pathway of lysine catabolism 245 6.2.4 Post-synthetic modifi cation of lysine in proteins 245 6.2.4.1 Hydroxylysine, lysine aldehyde (allysine) and cross-links in collagen and elastin 247 6.2.4.2 Methyl lysine 249 6.2.4.3 Pyrrolysine 251 6.2.5 Carnitine 252 6.3 Methionine and cysteine 255 6.3.1 Methionine biosynthesis 256 6.3.1.1 Cystathionine γ-synthase and cystathionine β-lyase 258 6.3.1.2 Methionine synthase 259 6.3.1.3 S-Methylmethionine in plants 260 6.3.2 S-Adenosylmethionine and the methylation cycle 260 6.3.2.1 Glycine N-methyltransferase 263 6.3.2.2 Megaloblastic anaemia and the methyl folate trap 264 6.3.2.3 Methionine γ-lyase 264 6.3.3 Transsulphuration and cysteine synthesis in animals 265 6.3.3.1 Homocystinuria, hyperhomocysteinaemia and cardiovascular disease 266 6.3.4 Ethylene synthesis in plants 268 6.3.5 Radical SAM enzymes 271 6.3.6 Hydrogen sulphide 272 6.3.7 Taurine and the catabolism of cysteine 273 Further reading 276 7 The Branched-Chain Amino Acids: Leucine, Isoleucine and Valine 279 7.1 Synthesis of the branched-chain amino acids 280 7.1.1 Acetohydroxyacid synthase 282 7.1.2 Acetohydroxyacid reducto-isomerase, dihydroxyacid dehydratase and transamination of the oxo-acids 283 7.1.3 Leucine synthesis 284 7.1.3.1 The pyruvate pathway of isoleucine synthesis 286 7.2 Mammalian catabolism of the branched-chain amino acids 287 7.2.1 Branched-chain amino acid transaminases 289 7.2.2 Branched-chain 2-oxo-acid dehydrogenase 290 7.2.2.1 Maple syrup urine disease 293 7.2.3 Branched-chain acyl CoA dehydrogenases 293 7.2.4 Leucine catabolism 295 7.2.5 Isoleucine catabolism 296 7.2.6 Valine catabolism 297 7.2.7 Biotin-dependent carboxylation reactions 299 7.2.7.1 Multiple carboxylase deficiency 300 Further reading 302 8 Histidine 305 8.1 Biosynthesis of histidine 306 8.2 Histidine catabolism 310 8.2.1 The urocanic acid pathway of histidine catabolism 311 8.2.1.1 The histidine load test (FIGLU test) for folate nutritional status 314 8.2.2 The hydantoin propionate pathway 315 8.2.3 The transaminase pathway of histidine catabolism 316 8.3 Histamine 316 8.3.1 Bacterial histamine poisoning (scombroid poisoning) 317 8.3.2 Histidine decarboxylase 318 8.3.3 Histamine catabolism 319 8.4 Methylhistidine 321 8.5 Carnosine and related histidine-containing peptides 321 Further reading 322 9 The Aromatic Amino Acids: Phenylalanine, Tyrosine and Tryptophan 323 9.1 Biosynthesis of phenylalanine, tyrosine and tryptophan 324 9.1.1 The shikimate pathway 325 9.1.2 Synthesis of phenylalanine and tyrosine 328 9.1.3 Synthesis of tryptophan 331 9.1.3.1 The trp operon 333 9.2 Metabolism of phenylalanine and tyrosine 335 9.2.1 Phenylalanine ammonia lyase and lignin biosynthesis in plants 335 9.2.2 Polyphenol biosynthesis in plants 338 9.2.3 Phenylalanine hydroxylase and phenylketonuria 339 9.2.4 The catecholamines: dopamine, noradrenaline and adrenaline 342 9.2.4.1 Parkinson’s disease and inhibitors of dopa decarboxylase 346 9.2.4.2 Catabolism of the catecholamines 346 9.2.5 Tyrosinase and melanin synthesis 349 9.2.6 The thyroid hormones, thyroxine and tri-iodothyronine 352 9.3 Catabolism of phenylalanine and tyrosine 355 9.4 Metabolism of tryptophan 357 9.4.1 Auxin (indoleacetic acid) 357 9.4.2 Indole formation 358 9.4.3 Serotonin and melatonin 359 9.4.3.1 Melatonin synthesis and catabolism 362 9.4.4 The kynurenine pathway of tryptophan metabolism 363 9.4.4.1 Regulation of tryptophan dioxygenase 365 9.4.4.2 Kynurenine metabolism 367 9.4.4.3 Kynureninase and the tryptophan load test for vitamin B6 nutritional status 368 9.4.4.4 De novo synthesis of NAD 369 9.4.5 Pellagra 370 9.4.5.1 The pellagragenic effect of excess dietary leucine 372 9.4.5.2 Inborn errors of tryptophan metabolism 372 9.4.5.3 Carcinoid syndrome 373 9.4.5.4 Drug-induced pellagra 373 9.5 Quinone cofactors in amine oxidases 374 Further reading 375 Bibliography 377 Index 431

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Book SynopsisProteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine.Table of ContentsPreface xi About the Companion Website xiii Chapter 1 Proteins and proteomics 1 1.1 Proteins, an introduction 1 1.2 Genes, genomics and proteomics 2 1.3 Bioinformatics 12 1.4 Proteomics: goals and applications 14 Further reading 22 Chapter 2 Protein structure and engineering 25 2.1 Primary structure 25 2.2 Higher-level structure 36 2.3 Protein classification on the basis of structure 41 2.4 Protein structural stability 45 2.5 Higher-order structure prediction 47 2.6 Protein folding 48 2.7 Intrinsically disordered proteins 50 2.8 Protein engineering 51 2.9 Protein post-translational modification 54 Further reading 62 Chapter 3 Protein sources 65 3.1 Recombinant versus non-recombinant production 65 3.2 Approaches to recombinant protein production 67 3.3 Heterologous protein production in E. coli 72 3.4 Heterologous production in bacteria other than E. coli 77 3.5 Heterologous protein production in yeast 77 3.6 Heterologous protein production in fungi 78 3.7 Proteins from plants 80 3.8 Animal tissue as a protein source 84 3.9 Heterologous protein production in transgenic animals 85 3.10 Heterologous protein production using animal cell culture 86 3.11 Insect cell culture systems 87 Further reading 88 Chapter 4 Protein purification and characterization 91 4.1 Protein detection and quantification 93 4.2 Initial recovery of protein 95 4.3 Removal of whole cells and cell debris 98 4.4 Concentration 103 4.5 Chromatographic purification 107 4.6 Protein inactivation and stabilization 128 4.7 Protein characterization 137 Further reading 139 Chapter 5 Large-scale protein production 141 5.1 Upstream processing 141 5.2 Downstream processing 154 5.3 Therapeutic protein production: some special issues 163 5.4 Range and medical significance of impurities potentially present in protein-based therapeutic products 166 Further reading 175 Chapter 6 Therapeutic proteins: blood products, vaccines and enzymes 177 6.1 Blood products 177 6.2 Anticoagulants 184 6.3 Thrombolytic agents 186 6.4 Additional blood-related products 189 6.5 Vaccine technology 190 6.6 Therapeutic enzymes 194 Further reading 202 Chapter 7 Therapeutic antibodies 205 7.1 Antibodies 205 7.2 IgG structure and activity 205 7.3 Antibody therapeutics: polyclonal antibody preparations 209 7.4 Antibody therapeutics: monoclonal antibodies 211 7.5 Therapeutic applications of monoclonal antibodies 220 7.6 Antibody conjugates 223 7.7 Bispecific antibodies 224 7.8 Antibody fragments 225 7.9 Engineering the antibody glycocomponent 228 7.10 Fc fusion proteins 229 Further reading 230 Chapter 8 Hormones and growth factors used therapeutically 233 8.1 Insulin 233 8.2 Glucagon 240 8.3 Gonadotrophins 240 8.4 Growth hormone 243 8.5 Erythropoietin 246 8.6 Other hormones 247 8.7 Growth factors 249 Further reading 253 Chapter 9 Interferons, interleukins and tumour necrosis factors 257 9.1 Regulatory factors: cytokines versus hormones 257 9.2 Interferons 258 9.3 Interleukins 264 9.4 Tumour necrosis factors 271 Further reading 274 Chapter 10 Proteins used for analytical purposes 277 10.1 The IVD sector 279 10.2 The basis of analyte detection and quantification 280 10.3 Enzymes as diagnostic/analytical reagents 281 10.4 Biosensors 289 10.5 Antibodies as analytical reagents 295 Further reading 309 Chapter 11 Industrial enzymes: an introduction 311 11.1 Sales value and manufacturers 313 11.2 Sources and engineering 314 11.3 Environmental benefits 315 11.4 Enzyme detection and quantification 315 11.5 Immobilized enzymes 316 11.6 Extremophiles 319 11.7 Enzymes in organic solvents 324 11.8 Industrial enzymes: the future 325 Further reading 325 Chapter 12 Industrial enzymes: proteases and carbohydrases 327 12.1 Proteolytic enzymes 327 12.2 Carbohydrases 340 Further reading 367 Chapter 13 Additional industrial enzymes 371 13.1 Lipases 371 13.2 Penicillin acylase 375 13.3 Amino acylase and amino acid production 378 13.4 Cyclodextrins and cyclodextrin glycosyltransferase 380 13.5 Enzymes and animal nutrition 382 13.6 Enzymes in molecular biology 387 Further reading 390 Chapter 14 Non-catalytic industrial proteins 393 14.1 Functional properties of proteins 393 14.2 Milk and milk proteins 397 14.3 Animal-derived proteins 408 14.4 Plant-derived proteins 411 14.5 Sweet and taste-modifying proteins 412 Further reading 414 Index 417

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Book SynopsisThis is a modern introductory book on sensors, combining underlying theory with bang up to date topics such as nanotechnology. The text is suitable for graduate students and research scientists with little background in analytical chemistry. It is user-friendly, with an accessible theoretical approach of the basic principles, and referencesfor further reading. Thebook covers up-to-date advances in the sensor field, e.g. nanotechnology and quantum dots. It includes calculation exercises and solutions, andthe accompanying website contains Powerpoint slides.Trade Review“Summary In conclusion it can be stated that this book is very suitable for students and a sound didactic means of learning the basics of chemo and biosensors . . . The organization of the content and the quantity of material presented are highly suitable for undergraduate and graduate students and for newcomers to this field; it can, therefore, be recommended for those wishing to gain both a first insight into, and a comprehensive overview of, this still growing topic.” (Analytical and Bioanalytical Chemistry, 1 March 2013)Table of Contents1 What are Chemical Sensors? 1 2 Protein Structure and Properties 21 3 Enzymes and Enzymatic Sensors 28 4 Mathematical Modeling of Enzymatic Sensors 50 5 Materials and Methods in Chemical-Sensor Manufacturing 66 6 Affinity-Based Recognition 101 7 Nucleic Acids in Chemical Sensors 118 8 Nanomaterial Applications in Chemical Sensors 135 9 Thermochemical Sensors 157 10 Potentiometric Sensors 165 11 Chemical Sensors Based on Semiconductor Electronic Devices 217 12 Resistive Gas Sensors (Chemiresistors) 246 13 Dynamic Electrochemistry Transduction Methods 258 14 Amperometric Enzyme Sensors 314 15 Mathematical Modeling of Mediated Amperometric Enzyme Sensors 332 16 Electrochemical Affinity and Nucleic Acid Sensors 347 17 Electrical-Impedance-Based Sensors 367 18 Optical Sensors – Fundamentals 404 19 Optical Sensors – Applications 435 20 Nanomaterial Applications in Optical Transduction 454 21 Acoustic-Wave Sensors 473 22 Microcantilever Sensors 507 23 Chemical Sensors Based on Microorganisms, Living Cells and Tissues 518

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Book SynopsisThis is a modern introductory book on sensors, combining underlying theory with bang up to date topics such as nanotechnology. The text is suitable for graduate students and research scientists with little background in analytical chemistry.Trade Review“Summary In conclusion it can be stated that this book is very suitable for students and a sound didactic means of learning the basics of chemo and biosensors . . . The organization of the content and the quantity of material presented are highly suitable for undergraduate and graduate students and for newcomers to this field; it can, therefore, be recommended for those wishing to gain both a first insight into, and a comprehensive overview of, this still growing topic.” (Analytical and Bioanalytical Chemistry, 1 March 2013)Table of Contents1 What are Chemical Sensors? 1 2 Protein Structure and Properties 21 3 Enzymes and Enzymatic Sensors 28 4 Mathematical Modeling of Enzymatic Sensors 50 5 Materials and Methods in Chemical-Sensor Manufacturing 66 6 Affinity-Based Recognition 101 7 Nucleic Acids in Chemical Sensors 118 8 Nanomaterial Applications in Chemical Sensors 135 9 Thermochemical Sensors 157 10 Potentiometric Sensors 165 11 Chemical Sensors Based on Semiconductor Electronic Devices 217 12 Resistive Gas Sensors (Chemiresistors) 246 13 Dynamic Electrochemistry Transduction Methods 258 14 Amperometric Enzyme Sensors 314 15 Mathematical Modeling of Mediated Amperometric Enzyme Sensors 332 16 Electrochemical Affinity and Nucleic Acid Sensors 347 17 Electrical-Impedance-Based Sensors 367 18 Optical Sensors -- Fundamentals 404 19 Optical Sensors -- Applications 435 20 Nanomaterial Applications in Optical Transduction 454 21 Acoustic-Wave Sensors 473 22 Microcantilever Sensors 507 23 Chemical Sensors Based on Microorganisms, Living Cells and Tissues 518 Index 531

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Book SynopsisSince the major pioneering of joint replacement surgery more than fifty years ago, much research and progress has been made in the field of arthroplasty with new insights into better materials, types of cement and bone-cell compatible coatings, and a better understanding of the causes of implant failure.Table of ContentsPreface xi 1 Introduction 1 References 5 2 Basic Anatomy 7 2.1 Terminology 7 2.2 Human Skeleton 8 2.3 Joints 10 2.4 Cartilage 10 2.5 Protein and Collagen 11 2.6 Human Bone 14 References 22 3 Anatomy of Joints 25 3.1 Shoulder 25 3.2 Elbow 29 3.3 Wrist 34 3.4 Finger 38 3.5 Hip 38 3.6 Knee 43 3.7 Ankle 49 3.8 Foot 52 3.9 Toe 52 3.10 Degradation of Joints 54 References 56 4 Methods of Inspection for Joint Replacements 59 4.1 Introduction 59 4.2 Gait Analysis 60 4.3 X-ray 61 4.4 Tomography and Computed Tomography (CT) 64 4.5 Radionuclide Scanning 66 4.6 Ultrasonography 66 4.7 Magnetic Resonance Imaging (MRI) 67 References 69 5 Materials in Human Joint Replacement 71 5.1 Introduction 71 5.2 Alloy Metals 71 5.3 Ceramics 79 5.4 Polymers 83 5.5 Joint Replacement Materials in Service 91 5.6 Nanomaterials 95 References 98 6 Methods of Manufacture of Joint Replacements 103 6.1 Introduction 103 6.2 Surface Finish 104 6.3 Tolerance 106 6.4 Wear and Friction 106 6.5 Machining 106 6.6 Forging 112 6.7 Casting 114 6.8 Manufacture of Polymer Parts 119 6.9 Surface Treatment 121 6.10 Surface Finishing of Implants 125 6.11 Manufacture of Joint Replacements 127 References 128 7 Computer-Aided Engineering in Joint Replacements 131 7.1 Introduction 131 7.2 Reverse Engineering 132 7.3 Solid Modelling 133 7.4 Finite Element Analysis (FEA) 137 7.5 Rapid Prototyping (RP) in Joint Replacement Manufacture 141 7.6 Computer-Aided Manufacture 145 7.7 Navigation 150 7.8 Robotics 153 References 162 8 Joint Replacement 167 8.1 Introduction 167 8.2 Shoulder 171 8.3 Elbow 175 8.4 Wrist 178 8.5 Fingers 180 8.6 Hip 183 8.7 Knee 191 8.8 Ankle 200 8.9 Foot and Toe 203 References 206 Index

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Book SynopsisAs will be seen, there is not much missing here. I thought that the sections were well balanced, with rarely too much or too little on a given topic...This is a text to be welcomed by both teachers and students. BIOCHEMISTRY & MOLECULAR BIOLOGY EDUCATION (on the first edition) The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. A major update of this comprehensive, accessible introduction to physical biochemistry. Includes two new chapters on proteomics and bioinformatics. Introduces experimental approaches with a minimum of mathematics and numerous practical examples. Provides a bibliography at the end of each chapter. Written by an author with Table of ContentsPreface. Chapter 1 Introduction. 1.1 Special Chemical Requirements of Biomolecules. 1.2 Factors Affecting Analyte Structure and Stability. 1.3 Buffering Systems Used in Biochemistry. 1.4 Quantitation, Units and Data Handling. 1.5 The Worldwide Web as a Resource in Physical Biochemistry. 1.6 Objectives of this Volume. References. Chapter 2 Chromatography. 2.1 Principles of Chromatography. 2.2 Performance Parameters Used in Chromatography. 2.3 Chromatography Equipment. 2.4 Modes of Chromatography. 2.5 Open Column Chromatography. 2.6 High Performance Liquid Chromatography (HPLC). 2.7 Fast Protein Liquid Chromatography. 2.8 Perfusion Chromatography. 2.9 Membrane-Based Chromatography Systems. 2.10 Chromatography of a Sample Protein. References. Chapter 3 Spectroscopic Techniques. 3.1 The Nature of Light. 3.2 The Electromagnetic Spectrum. 3.3 Ultraviolet/Visible Absorption Spectroscopy. 3.4 Fluorescence Spectroscopy. 3.5 Spectroscopic Techniques Using Plane-Polarized Light. 3.6 Infrared Spectroscopy. 3.7 Nuclear Magnetic Resonance (NMR) Spectroscopy. 3.8 Electron Spin Resonance (ESR) Spectroscopy. 3.9 Lasers. 3.10 Surface Plasmon Resonance. References. Chapter 4 Mass Spectrometry. 4.1 Principles of Mass Spectrometry. 4.2 Mass Spectrometry of Proteins/Peptides. 4.3 Interfacing MS with other Methods. 4.4 Uses of Mass Spectrometry in Biochemistry. References. Chapter 5 Electrophoresis. 5.1 Principles of Electrophoresis. 5.2 Nondenaturing Electrophoresis. 5.3 Denaturing Electrophoresis. 5.4 Electrophoresis in DNA Sequencing. 5.5 Isoelectric Focusing (IEF). 5.6 Immunoelectrophoresis. 5.7 Agarose Gel Electrophoresis of Nucleic Acids. 5.8 Pulsed Field Gel Electrophoresis. 5.9 Capillary Electrophoresis. 5.10 Electroblotting Procedures. 5.11 Electroporation. References. Chapter 6 Three-Dimensional Structure Determination of Macromolecules. 6.1 The Protein-Folding Problem. 6.2 Structure Determination by NMR. 6.3 Crystallization of Biomacromolecules. 6.4 X-Ray Diffraction by Crystals. 6.5 Calculation of Electron Density Maps. 6.6 Other Diffraction Methods. 6.7 Comparison of X-Ray Crystallography with Multi-Dimensional NMR. 6.8 Structural Databases. References. Chapter 7 Hydrodynamic Methods. 7.1 Viscosity. 7.2 Sedimentation. 7.3 Methods for Varying Buffer Conditions. 7.4 Flow Cytometry. References. Chapter 8 Biocalorimetry. 8.1 The Main Thermodynamic Parameters. 8.2 Isothermal Titration Calorimetry. 8.3 Differential Scanning Calorimetry. 8.4 Determination of Thermodynamic Parameters by Non-Calorimetric Means. References. Chapter 9 Bioinformatics. 9.1 Overview of Bioinformatics. 9.2 Sequence Databases. 9.3 Tools for Analysis of Primary Structures. 9.4 Tertiary Structure Databases. 9.5 Programs for Analysis and Visualization of Tertiary Structure Databases. 9.6 Homology Modelling. References. Chapter 10 Proteomics. 10.1 Electrophoresis in Proteomics. 10.2 Mass Spectrometry in Proteomics. 10.3 Chip Technologies in Proteomics. 10.4 Post-Translational Modification Proteomics. Further Reading. References. Appendix 1 SI Units. Appendix 2 The Fourier Transform. Index.

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Book SynopsisOffers a study guide which describes the basic principles of pharmaceutical physicochemical characterisation. This text introduces the basic concepts and discusses their wider implication for pharmaceutical development, with reference to many case examples of drugs and drug products.Table of ContentsPreface xiii List of Abbreviations xv 1 Basic Principles of Preformulation Studies 1 1.1 Introduction 1 1.2 Assay design 2 1.2.1 Assay development 3 1.3 Concentrations 5 1.3.1 Units of concentration 5 1.4 UV spectrophotometry 9 1.4.1 Method development for UV assays 11 1.5 Thin-layer chromatography (TLC) 14 1.5.1 TLC method development 15 1.5.2 High-performance TLC 17 1.6 High-performance liquid chromatography 19 1.6.1 Normal- and reverse-phase HPLC 20 1.6.2 HPLC method development 21 1.7 Differential scanning calorimetry 22 1.7.1 Interpreting DSC data 23 1.7.2 Modulated-temperature DSC 27 1.7.3 DSC method development 30 1.8 Dynamic vapour sorption 32 1.8.1 DVS method development 32 1.9 Summary 33 References 33 Answer to study question 34 Additional study questions 35 2 Ionisation Constants 36 2.1 Introduction 36 2.2 Ionisation 36 2.2.1 Percent ionisation 42 2.3 Buffers 44 2.4 Determination of pKa 44 2.4.1 Determination of pKa by potentiometric titration 45 2.4.2 Determination of pKa in nonaqueous solvents 45 2.4.3 Other factors affecting measurement of pKa 47 2.5 Summary 48 References 48 Answers to study questions 49 Additional self-study questions and answers 50 3 Partition Affinity 52 3.1 Introduction 52 3.2 Partitioning 52 3.2.1 Effect of partitioning 54 3.2.2 Determination of log P 55 3.2.3 Effect of salt formation on partitioning 62 3.3 Summary 63 References 63 Answers to study questions 64 4 Solubility 65 4.1 Introduction 65 4.2 Intrinsic solubility 67 4.2.1 Ideal solubility 69 4.2.2 Solubility as a function of temperature 73 4.2.3 Solubility and physical form 74 4.2.4 Measurement of intrinsic solubility 77 4.2.5 Calculation of pKa from solubility data 83 4.3 Summary 83 References 84 Answer to study question 84 Additional self-study questions and answers 84 5 Dissolution 86 5.1 Introduction 86 5.2 Models of dissolution 86 5.3 Dissolution testing 87 5.3.1 Intrinsic dissolution rate (IDR) 92 5.3.2 IDR as a function of pH 93 5.3.3 IDR and the common ion effect 94 5.4 Summary 96 References 96 6 Salt Selection 98 6.1 Introduction 98 6.2 Salt formation 99 6.2.1 Selection of a salt-forming acid or base 104 6.2.2 Salt screening 108 6.3 Salt solubility 110 6.3.1 Solubility of basic salts 111 6.3.2 Solubility of acidic salts 112 6.3.3 The importance of pHmax 114 6.4 Dissolution of salts 117 6.4.1 Modification of pHm 120 6.5 Partitioning of salts 121 6.6 Summary 123 References 124 Answers to study questions 126 7 Physical Form I – Crystalline Materials 127 7.1 Introduction 127 7.2 Crystal formation 127 7.2.1 Crystal formation from the melt 128 7.2.2 Crystal growth from solution 129 7.3 Crystal structure 130 7.4 Polymorphism 131 7.4.1 Thermodynamics of polymorphism 133 7.4.2 Physicochemical properties of polymorphs 137 7.5 Pseudopolymorphism 139 7.6 Polymorph screening 141 7.7 Characterisation of physical form 141 7.7.1 Characterisation of polymorphs 142 7.7.2 Characterisation of pseudopolymorphs 149 7.8 Summary 152 References 152 Answers to study questions 153 8 Physical Form II – Amorphous Materials 156 8.1 Introduction 156 8.2 Formation of amorphous materials 156 8.3 Ageing of amorphous materials 160 8.4 Characterisation of amorphous materials 162 8.4.1 Measurement of ageing 164 8.5 Processing and formation of amorphous material 168 8.5.1 Spray-drying 168 8.5.2 Freeze-drying 168 8.5.3 Quench-cooling 169 8.5.4 Milling 170 8.5.5 Compaction 171 8.6 Amorphous content quantification 171 8.6.1 Calibration standards 172 8.6.2 DSC for amorphous content quantification 173 8.6.3 DVS for amorphous content quantification 175 8.7 Summary 177 References 178 Answers to study questions 179 9 Stability Assessment 181 9.1 Introduction 181 9.2 Degradation mechanisms 183 9.2.1 Hydrolysis 185 9.2.2 Solvolysis 187 9.2.3 Oxidation 188 9.2.4 Photolysis 190 9.3 Reaction kinetics 191 9.3.1 Solution-phase kinetics 191 9.3.2 Zero-order reactions 192 9.3.3 First-order kinetics 193 9.3.4 Second-order reactions 194 9.3.5 Solid-state kinetics 195 9.4 The temperature dependence of reaction kinetics 198 9.5 Stress testing 203 9.5.1 Stress testing in solution 203 9.5.2 Stress testing in the solid-state 204 9.5.3 Drug–excipient compatibility testing 205 9.6 Summary 208 References 208 Answers to study questions 209 10 Particle Properties 211 10.1 Introduction 211 10.2 Microscopy 211 10.2.1 Light microscopy 212 10.2.2 Hot-stage microscopy 213 10.2.3 Electron microscopy 214 10.2.4 Atomic force microscopy 214 10.3 Particle shape 215 10.3.1 Habit 215 10.3.2 Particle sizing 219 10.3.3 Particle size distributions 222 10.4 Summary 227 References 227 Answer to study question 227 11 Powder Properties 228 11.1 Introduction 228 11.2 Powder flow and consolidation 228 11.2.1 Carr’s index 230 11.2.2 Hausner ratio 232 11.2.3 Angle of repose 232 11.2.4 Mohr diagrams 235 11.3 Compaction properties 240 11.3.1 Compaction simulators 242 11.4 Summary 243 References 243 Answers to study questions 243 Index 247 Companion website This book is accompanied by a companion website at: ttp://www.wiley.com/go/gaisford/essentials Visit the website for: • Figures and tables from the book • Multiple choice questions

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Book SynopsisPharmaceutical Emulsions: A Drug Developer's Toolbag covers all the key aspects of pharmaceutical emulsions, starting from the fundamental scientific basics, to the pharmaceutical forms and the chemical tests for its application.Table of ContentsMathematical symbols (with normal units) xi Acronyms and abbreviations xiii Preface xv Acknowledgements xvii About the companion website xix I Product considerations: medicinal formulations 1 1 Historical perspective 5 1.1 Landmarks 5 1.2 Significant discoveries 7 1.3 Difficulties 8 1.4 Traditional uses 10 1.5 Product regulation 13 2 What is an emulsion? 15 2.1 States of matter 24 2.2 Summary thermodynamics 34 2.3 Interfacial tension and wetting 36 2.4 Shear and size reduction 46 2.5 Raw materials 47 3 Stability, metastability and instability 49 3.1 Stokes’ law 51 3.2 Derjaguin–Landau–Verwey–Overbeek (DLVO) theory 52 3.3 Interfacial rheology 56 4 Manufacture 63 4.1 Premixing 63 4.2 High-shear mixers and size reduction 64 4.3 Multiple and microemulsions 65 4.4 Hot melt (steriles) 65 4.5 Filling 66 II Forms, uses and applications: biopharmaceutics 67 5 Creams and ointments 69 5.1 Nutraceuticals and cosmeceuticals 71 5.2 Medicinals 71 6 Pastes and bases 77 6.1 Emolliency 77 6.2 Suppositories 78 6.3 Pessaries 79 7 IV colloids 81 7.1 Needle free 86 7.2 Ocular therapy 87 7.3 Cancer 88 7.4 Antimicrobials 90 7.5 Temperature-sensitive matrices and release forms 93 7.6 Targeted endosomal use 94 7.7 Solid lipid nanoparticles 95 7.8 Diagnostic emulsions 98 8 Transdermal patches: semisolids 99 8.1 Hormones 104 8.2 Analgesia 104 8.3 Anaesthesia 104 8.4 Nicotine 105 8.5 Inserts: vaginal rings 105 9 Gels 107 9.1 Micro- and nanogels 107 9.2 Semisolids 107 10 Implants 109 10.1 Plastics and glasses 109 10.2 Thermoresponsive materials 110 11 De novo science, sustainable novel products and platform applications 111 11.1 Tablets 113 11.2 Metered-dose inhalers 113 11.3 Blood substitutes 114 III Tests: chemistry to control the quality, efficacy and fitness for purpose of a product 117 12 Physicochemical properties 119 12.1 Thermal evaluation (differential scanning calorimetry) and lipid polymorphs 124 12.2 Drug form, log P and Lipinski rules 129 12.3 Skin and epithelial models 133 12.4 Drug delivery routes 134 13 Sizing and microscopy 137 13.1 ζ -potential 137 13.2 Hydrodynamic diameter 139 14 Rheology, texture, consistency and spreadability 141 14.1 Bulk properties 141 14.2 Solid-state and nanorheological properties 144 14.3 Interfacial properties 144 15 Quality control, process analytical technology and accelerated testing 149 15.1 Preformulation, high-throughput screening 150 15.2 Industrial concerns 151 15.3 Rancimat and other methods 152 Questions 155 Guide for readers 155 Specimen 'test' questions 155 Answers 168 References 173 Index 181

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Book SynopsisBoasting numerous industrial applications, inorganic chemistry forms the basis for research into new materials and bioinorganic compounds such as calcium that act as biological catalysts. Now complete, this highly acclaimed series presents current knowledge in all areas of inorganic chemistry, including chemistry of the elements; organometallic, polymeric and solid-state materials; and compounds relevant to bioinorganic chemistry.

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Book SynopsisMany medical applications could benefit from system theory, if only instrumentation designers understood systems theory and signal processing academics had a better understanding of practical medical applications. This book bridges those gaps in a practical manner, with suggestions for coursework included.Trade Review"…this is a useful addition to the library of those who are involved in product development of technologies using complex signal processing." (Biomedical Instrumentation & Technology, May/June 2004) "...an excellent contribution to the current literature...well written..." (IEEE Engineering in Medicine and Biology, July/August 2002) "The approach chosen by Dr. Baura is original and is to be congratulated for its ambitiousness. I would recommend the book to existing biomedical engineering experts working in environment where solving practical problems is the issue...in addition it could be very useful as a class text..." (IFMBE News, No. 61, July 2003)Table of ContentsPreface. Nomenclature. I FILTERS. 1 System Theory and Frequency-Selective Filters. 2 Low Flow Rate Occlusion Detection Using Resistance Monitoring. 3 Adaptive Filters. 4 Improved Pulse Oximetry. 5 Time-Frequency and Time-Scale Analysis. 6 Improved Impedance Cardiography. II MODELS FOR REAL TIME PROCESSING. 7 Linear System Identification. 8 External Defibrillation Waveform Optimization. 9 Nonlinear System Identification. 10 Improved Screening for Cervical Cancer. 11 Fuzzy Models. 12 Continuous Noninvasive Blood Pressure Monitoring: Proof of Concept. III COMPARTMENTAL MODELS. 13 The Linear Compartmental Model. 14 Pharmacologic Stress Testing Using Closed-Loop Drug Delivery. 15 The Nonlinear Compartmental Model. 16 The Role of Nonlinear Compartmental Models in Development of Antiobesity Drugs. IV SYSTEM THEORY IMPLEMENTATION. 17 Algorithm Implementation. 18 The Need for More System Theory in Low-Cost Medical Applications. Glossary. Index.

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Book SynopsisThis new edition provides students and professionals with a comprehensive and up-to-date treatment of colloid science theory, methods, and applications. Emphasizing the molecular interactions that determine the properties of colloidal systems, the authors provide an authoritative account of critical developments in colloid science that have occurred over the past several decades. Combining all of the best features of a professional reference and a student text, the Second Edition features: * Concept maps preceding each chapter that put subject matter into perspective. * Numerous worked examples - many new to this edition - illustrating key concepts. * More than 250 high-quality illustrations that help clarify processes described. * A new chapter that integrates the development of colloid science and technology in the twentieth century with challenges facing the field today. The Colloidal Domain, Second Edition is an indispensable professional resource Trade ReviewFrom the reviews of the First Edition: "Very well written and brings a focus and a perspective that are not currently available in one convenient volume, especially one that is suitable for self-study or as a teaching tool."-Colloid and Interface Science From the reviews of the First Edition: "A revolutionary approach [to] writing an up-to-date text on 'The Colloidal Domain' and its origin in and impact on physics, chemistry, biology, and technology."-Advanced Materials From the reviews of the First Edition: "The authors should be congratulated for producing such a well written text that is full of illustrations and formulas." -Chemistry and IndustryTable of ContentsSolutes and Solvents, Self-Assembly of Amphiphiles. Surface Chemistry and Monolayers. Electrostatic Interactions in Colloidal Systems. Structure and Properties of Micelles. Forces in Colloidal Systems. Bilayer Systems. Polymers in Colloidal Systems. Colloidal Stability. Colloidal Sols. Phase Equilibra, Phase Diagrams, and Their Application. Micro- and Macroemulsions. Epilogue. Index.

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Book SynopsisIntroduces the fundamental biological processes that influence these sophisticated, procedures. This text provides details about molecular-level events that happen at the tissue-implant interface and explores material, biological, and physiological consequences of these events. It emphasizes the importance of the body's wound-healing response.Trade Review"...well illustrated with a glossary, end-chapter summaries, and references...materials, scientists, medical device designers and manufacturers, corrosion researchers and practitioners, surgeons, and clinicians will profit from reading this book." (CORROSION, February 2006) "…a concise, topical, and not overly technical hardbound…the strengths of this book are its crisp information and condensed summaries. The jewels of this book are the diagrams and tables." (Annals of Biomedical Engineering, Issue 31:11) "...delivers precisely what the authors intended...excellent book...great introduction...nicely complements existing texts..." (Advanced Materials, Vol 16(4), 17 Feb 2004) "This text would be of great use for faculty teaching courses on tissue-biomaterial interactions." (IEEE Engineering in Medicine and Biology, May/ June 2003) "...a pleasure to read...highly recommendable..." (Biomateria.com) "...excellent attention to detail…recommended for graduate students, faculty and researchers, and bioengineers and physicians." (Choice, Vol. 40, No. 6, February 2003)Table of ContentsPreface. Acknowledgments. Introduction. Biomaterials. Proteins. Protein-Surface Interactions. Blood-Biomaterial Interactions and Coagulation. Inflammation and Infection. The Immune System and Inflammation. Wound Healing. Biomaterial Surface and the Physiological Environment. Biocompatibility. Example 1: Opening Occluded Vessels: Vascular Grafts, Intimal Hyperplasia. Example 2: Replacing Joints and Teeth. Answers to Quiz Questions. Gloassry. Index.

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Book SynopsisThis is a revised and expanded edition of a major reference work offering complete coverage of Russian chemical terms, along with their English translations.Table of ContentsNot Obtainable.

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Book SynopsisThis Second Edition of the well--received work on design, construction, and operation of heat exchangers. Demonstrates how to apply theories of fluid mechanics and heat transfer to practical problems posed by design, testing, and installation of heat exchangers.Table of ContentsHeat Exchanger Types and Construction. Heat Exchanger Fabrication. Heat Transmission and Fluid Flow. Performance Estimation. Boiling Heat Transfer and Flow Stability. Heat Pipes. Fluidized Beds. Flow Distribution Problems. Stress Analysis. Service Life, Reliability, and Maintenance. General Design Considerations and Approaches. Liquid-to-Liquid Heat Exchangers. Gas-to-Gas Heat Exchangers. Liquid-to-Gas Heat Exchangers. Steam Generators. Condensers. Heat Exchangers for Liquid Metals and Molten Salts. Heat Exchangers Operating on Radiant Energy. Cooling Towers. Heat Exchanger Tests. Handbook. Nomenclature, Constants, and Conversion Factors. Physical Properties Affecting Heat Transfer. Fluid Flow and Pressure Drop. LMTD and Thermal Effectiveness. Heat Transfer. Geometric Data for Tube Bundles and Header Sheets. Dimensional and Related Data for Pipes, Tubes, and Fins. Stress Analysis. Cost Estimation. Index.

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Book SynopsisA comprehensive, up-to-date treatment of the biochemistry essential for an understanding of molecular and cellular biological processes. This third edition offers new units covering the chemistry of life, bioenergetics, energy transfer molecules, regulation of enzymes and reaction sequences, lab techniques for purification of proteins and nucleic acids, and lab techniques of molecular genetics. Also, each unit contains more applications to biological systems. The text provides a well-organized and rigorous approach suitable for classroom use or self-instruction. Each unit begins with a 1- to 2-page presentation of basic concepts, followed by about 20 questions and problems with sample responses. Self-tests appear after every 2 to 3 units and there is a cumulative self-test at the end of the book.Table of ContentsIntroduction to the Chemistry of Life. Particles, Atoms, and Molecules. Chemical Groups. Ions, Ionic Bonds, and Crystals. Polarity, Hydrogen Bonds, Solubility, and HydrophobicInteractions. Chemical Equilibrium. Carbohydrates I: Monosaccharides and Related Molecules. Carbohydrates II: Disaccharides, and Polysaccharides. Amino Acids. Proteins I: Peptide Bonding and Polypeptides. Proteins II: Levels of Structure and Conjugated Proteins. Lipids: Fats, Phospholipids, Sterols, and Prostaglandins. Nucleic Acids. Bioenergetics. Energy Transfer Molecules. Enzymes. Regulation of Enzymes and Reaction Sequences. Molecular Weight and Molar, Percent, Mg Percent, and ppmSolutions. pH and H?+ Concentration. Weak Acids, Dissociation Constants, and Buffers. Self-Test. Laboratory Methods. Cumulative Self-Test. Index.

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Book SynopsisFunctional Biochemistry in Health and Disease provides aclear and straightforward account of the biochemistry that isnecessary to understand the physiological functions of tissues ororgans essential to the life of human beings.Trade Review"This text and resource will appeal to biochemists looking to better translate their knowledge and undersatnding into real situations. It will particularly appeal to physiologists and medical students hoping to unravel the biochemical processes that underpin the physiology of life." (ChemBioChem, 11 April 2011) "Functional Biochemistry in Health and Disease provides a clear and straightforward account of the biochemistry that is necessary to understand the physiological functions of tissues or organs essential to the life of human beings'. The book makes a strong connection between biochemistry and medical practice." (Australian Biochemist, December 2010)Table of ContentsAbbreviations. I INTRODUCTION. 1 The Structural and Biochemical Hierarchy of a Cell and a Human. Cell structure. Tissues. The whole human. The biochemical hierarchy. II ESSENTIAL TOPICS IN DYNAMIC BIOCHEMISTRY. 2 Energy: In the Body, Tissues and Biochemical Processes. Energy transformations in the whole body. Energy transformations in tissues and organs. Energy transformation in biochemical reactions and pathways. Adenosine triphosphate: its role in the cell. 3 Enzymes: Activities, Properties, Regulation and Physiology. Nomenclature and classifi cation. Basic facts. Mechanisms by which an enzyme enhances the rate of a reaction. Cofactors and prosthetic groups. Factors that change the activity of an enzyme. Allosteric inhibition. The physiological signifi cance of Km and Vmax values. Enzymes as tools. Enzymes in diagnosis. Enzymes as therapeutic agents. Enzymes as targets for therapy. Kinetic structure of a biochemical pathway. Regulation of enzyme activity. 4 Transport into the Body: The Gastrointestinal Tract, Digestion and Absorption. Gross structure of the gastrointestinal tract. Biochemistry of cooking and food preparation. Digestion and absorption. The gastrointestinal tract and disease. 5 Transport into the Cell: Particles, Molecules and Ions. Structure of the plasma membrane. Diffusion through membranes. Active transport. Endocytosis and exocytosis. Physiological importance of some transport systems. III ESSENTIAL METABOLISM. 6 Carbohydrate Metabolism. Glycolysis. The biochemical and physiological importance of anaerobic glycolysis. Regulation of the flux through glycolysis. Glycogen synthesis. Synthesis of Fructose and lactose. The pentose phosphate pathway. Gluconeogenesis: glucose formation from non-carbohydrate sources. Role of the liver in the regulation of the blood glucose concentration. Hormones and control of gluconeogenesis. Regulation of glycolysis and gluconeogenesis by ATP/ADP concentration ratio in the liver. Hypoglycaemia. Hyperglycaemia. 7 Fat Metabolism. Fats in nutrition. Fat fuels. Physiological importance of fat fuels. Limitations or drawbacks of fats as a fuel. Genetic defects in fatty acid oxidation. Pathological concentrations of fat fuels. 8 Amino Acid and Protein Metabolism. Introduction. Sources of amino acids. Protein and amino acid requirements. Fate of amino acids. Central role of transdeamination. Amino acid metabolism in different tissues. Glutamine: an amino acid of central importance. Urea 'salvage'. 9 Oxidation of Fuels and ATP Generation: Physiological and Clinical Importance. The Krebs cycle. The electron transfer chain. Oxidative phosphorylation. Coupling of electron transfer with oxidative phosphorylation. Transport into and out of mitochondria. 'Energy' transport in the cytosol: the creatine/phosphocreatine shuttle. Regulation of fl uxes. The physiological importance of mitochondrial ATP generation. The effect of ageing on ATP generation. 10 Metabolism of Ammonia and Nucleic Acids. Roles of ammonia. Urea synthesis. Degradation of nucleic acids, nucleotides, nucleosides and bases: the generation of ammonia. Ammonia toxicity. Defi ciencies of urea cycle enzymes. 11 Synthesis of Fatty Acids, Triacylglycerol, Phospholipids and Fatty Messengers: The Roles of Polyunsaturated Fatty Acids. Synthesis of long-chain fatty acids. Unsaturated fatty acids. Essential fatty acids. Phospholipids. Fatty messenger molecules. Fatty acids in neurological and behavioural disorders. 12 Hormones: From Action in the Cell to Function in the Body. Endocrine hormones: traditional and novel. The action, effects and functions of a hormone. Action of hormones. The biochemical and physiological effects of a hormone. Pheromones. Kinetic principles that apply to hormone action. IV ESSENTIAL PROCESSES OF LIFE. 13 Physical Activity: In Non-Athletes, Athletes and Patients. The mechanical basis of movement by skeletal muscle. Structure of muscle. Proteins involved in muscle action. Mechanism of contraction: the cross-bridge cycle. Regulation of contraction. Fuels for muscle. Fuels for various athletic events and games. Fatigue. Fatigue in patients. Physical training. Development of muscle. Health benefi ts of physical activity. Health hazards of physical activity. Skeletal muscle diseases. 14 Mental Activity and Mental Illness. Mental activity. Cells in the brain. Electrical communication. Chemical communication. Fuels and energy metabolism in the brain. Mental illnesses: biochemical causes. Recreational drugs. 15 Nutrition: Biochemistry, Physiology and Pathology. Basic information required for discussion of some biochemical aspects of nutrition. Vitamins. Minerals. A healthy diet. Nutrition for specifi c activities or conditions. Overnutrition. Malnutrition. Functional foods and nutraceuticals. Nutrition for patients with genetic disorders. Vegetarian diets. Eating disorders. 16 Starvation: Metabolic Changes, Survival and Death. Mechanisms for the regulation of the blood glucose concentration. Metabolic responses to starvation. Sequence of metabolic changes from intermediate starvation to death. Progressive decrease in protein degradation in starvation. 17 Defence Against Pathogens: Barriers, Enzymes and the Immune System. When the physical barrier is breached. The immune system. Adaptive immunity. Cytokines. Mechanisms for killing pathogens. Killing of intracellular bacteria and large parasites in the extracellular fluid. Allergy. Fuels and generation of ATP in immune cells: consequences for a patient. Essential fatty acids and proliferation. The lymph nodes. Tolerance. Chronic infl ammation and autoimmunity. Immunosuppressive agents. Conditions that reduce the effectiveness of the immune system. Factors that increase the effectiveness of the immune system. Return of the 'old' infectious diseases. New infectious diseases. Defence in the intestine. 18 Survival after Trauma: Metabolic Changes and Response of the Immune System. Physiological and metabolie responses the ebb & fl ow phases. Nutrition. Mobilisation of triacylglycerol and protein in trauma. Metabolic changes in trauma and in starvation. Fever. Summary of the effects of trauma on the immune system and the whole body. 19 Sexual Reproduction. Male reproductive system. Female reproductive system. The menstrual cycle. Ovulation. Chemical communication in male and female reproduction. Coitus and the sexual response in the male and female. Fertilisation. Pregnancy. Parturition. Contraception. The menopause. Sexually transmitted diseases. 20 Growth and Death of Cells and Humans: The Cell Cycle, Apoptosis and Necrosis. Introduction to cell proliferation. The cell cycle. Death. V SERIOUS DISEASES. 21 Cancer: Genes, Cachexia and Death. Basic information. Oncogenes and proto-oncogenes. Proteins expressed by oncogenes. Processes by which proto-oncogenes can be activated or converted to oncogenes. Tumour suppressor genes. Telomeres and telomerase in tumour cells. Metastasis. Metabolic changes in cancer patients. Overview of cancer. Cancer-causing agents or conditions. Chemotherapy. Radiotherapy. 22 Atherosclerosis, Hypertension and Heart Attack. Atherosclerosis. Hypertension. Heart attack (myocardial infarction). Index.

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Book SynopsisTable of ContentsThe Use of Plant Remedies in Indigenous Medical Systems. Presentation of Results. Preparation of Plant Material. The Gastro-Intestinal Tract. The Liver and Biliary System. The Cardiovascular System. The Respiratory System. Anti-Inflammatory and Analgesic Activity. Diabetes Mellitus. The Nervous System. Endocrine Activity: Antifertility and Sex Hormones. Appendices. Index.

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Book SynopsisEndocrine and Hormonal Toxicology Edited by Philip W. Harvey, Kevin C. Rush and Andrew Cockburn AgrEvo UK Ltd, Saffron Walden, UK This is the first book to consider the integrated role of the classical endocrine system and hormones (including those from tissues outside the classical endocrine system) in toxicological responses. Although focusing on the latest knowledge on endocrine glands as target organs and the mechanistic and molecular basis for toxicity in these organs, Endocrine and Hormonal Toxicology has been written to cover toxicological responses at the whole body level mediated by endocrine or hormonal mechanisms. This whole body, multi-organ approach significantly broadens the relevance of this volume to toxicologists. Following an introductory section on the types of endocrine toxicity including primary, secondary and indirect mechanisms, the next section deals with endocrine organs as toxicological targets. International contributions focus on the pituitary, thyroid and pTrade Review"section III of this book is a classic" (Clinical Endocrinology, No.53 2000)Table of ContentsSection 1: An Integrated Approach to Endocrine and Hormonal Toxicology Section 2: Endocrine Organs as Toxicological Targets Section 3: Endocrine and Hormonal Toxicology: A Target System Approach Section 4: Endocrine Toxicology: Human and Environmental Health Perspectives

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Book SynopsisCurrent Toxicology Series Series Editors Diana Anderson BIBRA Toxicology International, Surrey, UK Michael D Waters Consultant, Chapel Hill, NC, USA Timothy C Marrs Department of Health, London, UK Nutrition and Chemical Toxicity Edited by Costas Ioannides, University of Surrey, Guildford, UK An enormous amount of research is currently devoted to evaluating the role of nutrition in the aetiology of major human diseases and to clarifying the underlying mechanisms. This resurgent interest reflects the realisation that carcinogens may be generated during the normal cooking of food and that they may play a role in human carcinogenesis. Moreover, during the last decade it has become apparent that naturally occurring chemicals in food, primarily of plant origin, can afford effective protection against human cancer and heart disease. As a result, the need for up-to-date information in this subject area has never been greater. Nutrition and Chemical Toxicity deals in depth with the various toxTrade Review"The book is a well structured reference source for nutritional toxicologists, with many clear and concise diagrams, and a comprehensive bibliography at the end of each chapter." (British Toxicology Society Newsletter)Table of ContentsToxicants in Food: Naturally Occurring (H. Mori, et al.). Toxicants in Food: Fungal Contaminants (J.-S. Wang, et al.). Toxicants in Food: Generated During Cooking (K. Skog & M. Jägerstad). Toxicants in Food: Food Allergens (G. Reese & S. Lehrer). Nutritional Modulation of Cytochromes P450 (C. Ioannides). Interactions between Drugs and Diet (J. Thomas, et al.). Glutathione, Sulphur Amino Acids and Chemical Detoxication (T. Bray, et al.). Modulation of the Carcinogenic Response by Caloric Restriction (A. Turturro, et al.). Lipotropes and Chemical Carcinogenesis (E. Farber & A. Ghoshal). Expression of Chemical Toxicity in Vitamin Deficiency and Supplementation (G. Williamson). Safety Evaluation of Vitamins and Minerals (J. Hathcock). Naturally Occurring Organosulphur Compounds as Potential Anticarcinogens (H. Mori & A. Nishikawa). Cancer Chemoprevention by Tea Polyphenols (N. Ahmad, et al.). Animal Diets in Safety Evaluation Studies (G. Rao & J. Knapka). Index.

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Book SynopsisA catalyst is a substance which alters rate at which a chemical reaction occurs but is itself unchanged at the end of the reaction. This book includes approximately 40 proceduces describing the use of different catalysts.Trade Review"Everyone who is concerned with...pure compounds will be able to use this handy book..." (Angewandte Chemie, International Edition, Vol. 42, 2003) "...the book clearly fulfills its purpose to provide a practical introduction?it will be useful..." (CATTECH, August 2003)Table of ContentsSeries Preface. Preface to Volume 1. Abbreviations. PART I: REVIEW. 1. The Integration of Biotransformations into the Catalyst Portfolio. PART II: PROCEDURES. 2. General Information. 3. Asymmetric Epoxidation. 4. Epoxidation of , -Unsaturated Carbonyl Compounds. 5. Epoxidation of Allylic Alcohols. 6. Epoxidation of Unfunctionalized Alkenes and , -Unsaturated Esters. 7. Asymmetric Hydroxylation and Aminohydroxylation. 8. Asymmetric Sulfoxidation. 9. Asymmetric Reduction of Ketones Using Organometallic Catalysts. 10. Asymmetric Reduction of Ketones Using Baker's Yeast. 11. Asymmetric Reduction of Ketones Using Nonmetallic Catalysts. 12. Asymmetric Hydrogenation of Carbon-Carbon Double Bonds Using Organometallic Cataluysts. 13. Employment of Catalysts Working in Tandem. Index.

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Book SynopsisQuantitative Molecular Pharmacology and Informatics in Drug Discovery Michael Lutz, Section Head, Cheminformatics Group and Terry Kenakin, Principal Research Scientist, Glaxo Wellcome Research and Development, Research Triangle Park, NC, USA Quantitative Molecular Pharmacology and Informatics in Drug Discovery combines pharmacology, genetics and statistics to provide a complete guide to the modern drug discovery process. The book discusses the pharmacology of drug testing and provides a detailed description of the statistical methods used to analyze the resulting data. Application of genetic and genomic tools for identification of biological targets is reviewed in the context of drug discovery projects. Covering both the theoretical principles upon which the techniques are based and the practicalities of drug discovery, this informative guide. * outlines in step-by-step detail the advantages and disadvantages of each technology and approach and links these to the type of chemicalTrade Review"...a timely and important book..." "I recommend this book to pharmaceutical company statisticians..." --Biometrics, September 2000 "...covers a tremendous amount of ground....I recommend this book to pharmaceutical statisticians..." --Technometrics, 2000Table of ContentsDrug Discovery. Measurement of Drug Affinity. Efficacy. Pharmacological Assays Used in Screening for Therapeutic Ligands. Finding the Optimal Assay Format for the Chemical Target. Mathematical and Statistical Framework for Problems in Drug Discovery. Statistical Methods for Target Identification and Validation. Experimental Design. Analysis and Interpretation of Data. Index.

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Book SynopsisChemical Biophysics provides an engineering-based approach to biochemical system-analysis for graduate level courses on systems biology, computational bioengineering and molecular biophysics. It was the first textbook to apply rigorous physical chemistry principles to mathematical and computational modelling of biochemical systems for an interdisciplinary audience.Trade ReviewReview of the hardback: 'There are a growing number of physicists, engineers, mathematicians and chemists who are interested in joining the post-genomics party and addressing cutting-edge problems in molecular and cell biology. The barrier to entry can be high and prohibitive. This marvelous new book opens the door for the quantitively inclined. Beard and Qian, in an accessible and clear style, present fundamental methods that can be used to model and analyze an array of biomolecular systems and processes, ranging from enzyme kinetics to gene regulatory networks to cellular transport. This book will appeal to autodidacts as well as professors looking for course texts.' J. J. Collins, Professor of Biomedical Engineering and MacArthur Fellow, Boston UniversityReview of the hardback: 'This is one of the most useful and readable accounts of biochemical thermodynamics that I have seen for a long time, if indeed ever. It is very definitely a book that I shall want to have on my shelves and to refer others to, because it contains a considerable amount of information not easy to find elsewhere.' Athel Cornish-Bowden, Directeur de Recherche, CNRS, MarseillesReview of the hardback: 'Dan Beard's and Hong Qian's Chemical Biophysics is a masterful portrayal of a critically important new area of science. The success of genomics now makes it imperative to understand the relationships between proteomics, biochemical systems behavior and the physiology of the intact animal or human. This book provides the path. Its clarity of description, making the complexities seem simple by adhering to fundamental principles, avoiding cluttering detail while painting the broad picture to great depth, makes it a pleasure to read and a treasure to study. It's a must for scientists and scholars working to understand integrative biology.' James B. Bassingthwaighte, Professor of Bioengineering, Biomathematics and Radiology at the University of Washington, SeattleReview of the hardback: 'This wonderful book will be indispensable to specialists in the fields of systems biology, biochemical kinetics, cell signaling, genetic circuits and quantitative aspects of biology, and also to undergraduate and graduate students. It presents a systematic approach to analyzing biochemical systems. The complex subjects are described in a clear style, with carefully crafted definitions and derivations. This unique book is an important step in the development and dissemination of systems biology approaches.' Aleksander S. Popel, Professor of Biomedical Engineering, Johns Hopkins UniversityReview of the hardback: 'Chemical Biophysics: Quantitative Analysis of Cellular Systems by Daniel Beard and Hong Qian fills a significant niche. The text is a concise yet clear exposition of the fundamentals of chemical thermodynamics and kinetics, aimed specifically at practitioners of the new science of systems biology. It is marvellously illustrated with biochemical examples that will aid those who aim to analyze and model the workings of biological cells.' David Eisenberg, Director UCLA-DOE Institute for Genomics & Proteomics, University of California, Los Angeles, Investigator, Howard Hughes Medical InstituteReview of the hardback: 'As computational biology moves into a more integrative and multi-scale phase, to provide the quantitative framework for linking the mass of experimental data generated by molecular techniques at the subcellular level to tissue and organ scale physiology, it is vitally important that models are based on quantitative approaches that incorporate, wherever possible, thermodynamically constrained biophysical mechanisms. This new book on the Chemical Biophysics of Cellular Processes by Dan Beard and Hong Qian does a wonderful job of formulating models for metabolic pathways, gene regulatory networks and protein interaction networks on the well-established principles of physical chemistry. Topics include enzyme catalyzed reactions, reaction-diffusion modelling, membrane transport, the chemical master equation and much more. This book will be of lasting value to computational biologists and bioengineers.' Professor Peter J Hunter, Auckland Bioengineering Institute at the University of AucklandReview of the hardback: 'It is recommended for institutional or personal purchase as it comprises a substantial reference textbook.' Microbiology TodayTable of ContentsPreface; Introduction; Part I. Background Material: 1. Concepts from physical chemistry; 2. Conventions and calculations for biochemical systems; 3. Chemical kinetics and transport processes; Part II. Analysis and Modeling of Biochemical Systems: 4. Enzyme-catalyzed reactions; 5. Biochemical signaling modules; 6. Biochemical reaction networks; 7. Coupled biochemical systems and membrane transport; Part III. Special Topics: 8. Spatially distributed systems and reaction-diffusion modeling; 9. Constraint-based analysis of biochemical systems; 10. Biomacromolecular structure and molecular association; 11. Stochastic biochemical systems and the chemical master equation; 12. Appendix: the statistical basis of thermodynamics; Bibliography; Index.

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Book SynopsisBioenergetics is the study of the way biological systems, usually at the molecular level, utilize and convert energy in order to drive the biochemical reactions that constitute life. However, because of its often quantitative basis and the amount of technical jargon, the subject tends to alienate and intimidate students. This beautifully illustrated text has a lucid and logical approach to the subject. The text uses the modern perspective throughout so that the student is given an easily assimilable, logical introduction to the important concepts of the subject, particulary the core concept, the ''chemiosmotic theory''. It has been specifically designed to make information easily accessible by devoting each double-page spread to one topic. Within the spread, a variety of carefully constructed diagrams present information in a concise and innovative manner. The text is further enhanced by a comprehensive guide to additional reading. Original, easily understood combinatioTable of ContentsScope of bioenergetics. The 'universal' view of energy. The 'local' view of energy. Equilibrium and equilibrium constants. Sources of biological energy - quantitation. Direct coupling of oxidation to anhydride formation. Oxygen as an oxidising agent. Respiratory carriers. Respiratory chain (mitochondrial). Respiratory assemblages (mitochondrial). Mechanism of electron flow between proteins. Variations within respiratory chains. Light trapping and water splitting in chloroplasts. Reaction centre structure. Organisation of membrane proteins (chloroplast). Photosynthetic bacteria. Concepts of energy transduction- redox-anhydride bond energy. Transfer via H+gradient -. I. Qualitative aspects. II. Kinetic aspects. Quantitative aspects -. I. H+/O ratio. II. DGp/DMH+. How does electron transfer generate H+ (I) + (II). ATP synthase complex. F1 structure. F0 structure. Coupling H+ gradient to ATP synthesis - energetics. structural aspects. Integration with cytoplasm - mitochondria. Aspects of control - qualitative:quantitative. Integration of cytoplasm/control - chloroplasts. Uncouplers and ionophores in studies of phosphorylation. Mitochondrial defects. Alternative uses of H+ gradient. Alternative gradient production. ATP driven ion pumps - a broader perspective. E-P pumps. Evolution of pumps. Evolution of organelles

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Book SynopsisOffers guidelines that can reduce the risk or mitigate the severity of accidents associated with storing and handling reactive materials. Necessary elements of a reliable system to prevent equipment or human failures that might lead to a reactive chemical incident are sound and responsible management policies.Table of ContentsPreface. Acknowledgments. Acronyms. Introduction. 1. Chemical Reactivity Hazards. 1.1 Framework for Understanding Reactivity Hazards. 1.1.1 Grouping of Reactivity Hazards into General Categories. 1.1.2 Key Parameters That Drive Reactions. 1.1.3 Types of Runaway Reactions. 1.1.4 How Reactive Chemical Storage and Handling Accidents Are Initiated. 1.2 Self-Reactive Polymerizing Chemicals. 1.2.1 Thermal Instability. 1.2.2 Induction Time. 1.2.3 Example. 1.3 Self-Reactive Decomposing Chemicals. 1.3.1 Peroxides. 1.3.2 Self-Accelerating Decomposition Temperature. 1.3.3 Predicting Instability Potential. 1.3.4 Deflagration and Detonation of Pure Material. 1.3.5 Slow Gas-Forming Reactions. 1.3.6 Heat of Compression. 1.3.7 Minimum Pressure for Vapor Decomposition. 1.3.8 Shock Sensitivity. 1.3.9 Examples of Shock Sensitivity. 1.4 Self-Reactive Rearranging Chemicals. 1.4.1 Isomerization. 1.4.2 Disproportionation. 1.5 Reactivity with Oxygen. 1.5.1 Spontaneous Ignition and Pyrophoricity. 1.5.2 Pyrophoricity versus Hypergolic Properties. 1.5.3 Accumulation and Explosion of Pyrophoric Materials. 1.5.4 Competition between Air and Atmosphere Moisture. 1.5.5 Peroxide Formation. 1.6 Reactivity with Water. 1.6.1 Water Reactivity: Fast and Slow Reactions. 1.6.2 Water-Reactive Structures. 1.7 Reactivity with Other Common Substances. 1.7.1 Reactions with Metals. 1.7.2 Surface Area Effects. 1.7.3 Catalyst Deactivation and Surface Passivation. 1.8 Reactive with Other Chemicals Incompatibility. 1.8.1 Oxidizing and Reducing Properties. 1.8.2 Acidic and basic Properties. 1.8.3 Formation of Unstable Materials. 1.8.4 Thermite-Type Reactions. 1.8.5 Incompatibility with Heat Transfer Fluids and Refrigerants. 1.8.6 Adsorbents. References. 2. Chemical Reactivity Classifications. 2.1 NFPA Reactivity Hazard Signal. 2.1.1 NFPA 704 Rating System for Overall Reactivity. 2.1.2 Definitions for Reactivity Signal Ratings. 2.1.3 Reactivity Hazards Not Identified by NFPA 704. 2.1.4 NFPA Reactivity Ratings for Specific Chemicals. 2.2 NPCA Hazardous Materials Identification System. 2.3 Classifications of Organic Peroxides. 2.3.1 SPI 19A Classification of Organic Peroxides. 2.3.2 NFPA 43B Classification of Organic Peroxides. 2.4 Classification of Materials That Form Peroxides. 2.5 Classification of Water-Reactive Materials. 2.5.1 Materials That React Violently with Water. 2.5.2 Materials That React Slowly with Water. References. 3. Materials Assessment. 3.1 Prior Experience Review. 3.1.1 Common Knowledge. 3.1.2 Analogy. 3.1.3 Safety Data and Literature. 3.2 Theoretical Evaluations. 3.2.1 Unstable Atomic Groups. 3.2.2 Oxygen Balance. 3.2.3 Thermodynamics: Heat of 3.2.4 Thermodynamics: Heats of Reaction and Self-Reaction. 3.2.5 Thermodynamics: Equilibrium Considerations. 3.2.6 CHETAH. 3.2.7 Example Evaluation. 3.3 Expert Determination. 3.3.1 Expert Committees. 3.3.2 Kinetics Determination Factors. 3.4 Reactivity Screening Tests. 3.4.1 Thermal Stability Screening Tests. 3.4.2 Shock Sensitivity Screening. 3.4.3 Pyrophoricity Screening. 3.4.4 Water Reactivity Screening. 3.4.5 Peroxide Formation Screening. 3.4.6 Compatibility Screening. References. 4. Consequence Analysis. 4.1 Identifying Potential Accident Scenarios. 4.1.1 Process Hazard Analysis. 4.1.2 Checklist of Potentially Hazardous Events. 4.1.3 Chemical Interaction Matrix. 4.1.4 Industry Experience. 4.1.5 Local Size Experience. 4.2 Severity Testing. 4.2.1 Calorimetric Testing for Consequence Analysis. 4.2.2 Self-Accelerating Decomposition Temperature. 4.2.3 Isoperibolic Calorimetry. 4.2.4 Assessment of Maximum Pressure and Temperature. 4.3 Where to Find Methods for Estimating Immediate Consequences. 4.3.1 Reactive Chemical Explosions. 4.3.2 Reactive Chemical Fires. 4.3.3 Toxic Releases. 4.4 Where to Find Methods for Estimating Immediate Impact. 4.4.1 Explosion Effect Models. 4.4.2 Thermal Effect Models. 4.4.3 Toxic Gas Effect Models. 4.4.4 Modeling Systems. 4.4.5 Caveats. 4.5 Applications of Consequence Analysis. 4.5.1 Selection of Size, Quantity, and Location of Facilities. 4.5.2 Selection of Dedicated Safeguard Systems. 4.5.3 Basis for Emergency Response Systems and Planning. 4.5.4 Better Understanding of the Hazard and the Consequences. 4.5.5 Significant Step toward a Well-Managed Operating Facility. References. 5. General Design Considerations. 5.1 Summary of General Design Strategies. 5.1.1 Reduce the Inherent Hazards. 5.1.2 Build Reliable Safety Layers. 5.1.3 Conduct In-Depth Reviews. 5.1.4 Use Previous Experience. 5.2 Compatibility. 5.2.1 Identifying Potential Incompatibility Problems. 5.2.2 Compatibility with Process Materials/Reagents. 5.2.3 Compatibility with Impurities. 5.2.4 Compatibility with Heat Transfer Fluids. 5.2.5 Compatibility with Materials of Construction and Corrosion Products. 5.2.6 Compatibility with Insulation. 5.2.7 Compatibility with Fire-Extinguishing Agents. 5.2.8 Compatibility with Other Materials. 5.2.9 Other Compatibility-Related Practices. 5.3 Storage Time and Shelf Life. 5.3.1 Storage Time Limitations. 5.3.2 Practices for Increasing Shelf Life. 5.3.3 Handling and Disposal of Too-Old Material. 5.4 Storage Quantity and Configuration. 5.4.1 Determining Maximum Inventory. 5.4.2 Storage Configurations. 5.4.3 Top versus Bottom Discharge. 5.4.4 Facility Siting. 5.4.5 Restrictions on Container Shape or Configuration. 5.4.6 Mixing and Recirculation. 5.5 Air and Moisture Exclusion. 5.5.1 Air Exclusion Practices. 5.5.2 Moisture Exclusion Practices. 5.6 Monitoring and Control. 5.6.1 Oxygen Concentration Monitoring. 5.6.2 Humidity/Moisture Content Monitoring. 5.6.3 Pressure Monitoring. 5.6.4 Temperature Monitoring. 5.6.5 Temperature Control. 5.7 Handling and Transfer. 5.7.2 Piping Specifications and Layout. 5.7.3 Fittings and Connections. 5.7.4 Pumps and Pump Seals. 5.7.5 Valves. 5.7.6 Drain Systems. 5.7.7 Cleaning Equipment. 5.7.8 Transfer Systems Operating and Maintenance Practices. 5.8 Last-Resort Safety Features. 5.8.1 Inhibitor Injection. 5.8.2 Quench System. 5.8.3 Dump System. 5.8.4 Depressuring System. 5.8.5 Emergency Relief Configuration. 5.8.6 Emergency Relief Sizing Basis. 5.8.7 Emergency Relief Headers. 5.8.8 Emergency Relief Treatment Systems. 5.8.9 Explosion Suppression. 5.9 Passive Mitigation. 5.9.1 Flow-Limiting Orifices. 5.9.2 Fire-Resistant/Explosion-Resistant Construction. 5.9.3 Weak Seams and Explosion Venting. 5.9.4 Bunkers, Blast Walls and Barricades. 5.9.5 Secondary Containment. 5.9.6 Separation Distances. 5.10 Detections, Warning and Isolation. 5.10.1 Release Detection. 5.10.2 Release Warning. 5.10.3 Release Isolation. 5.11 Fire Prevention and Protection. 5.11.1 Ignition Source Control. 5.11.2 Fireproofing and Insulation. 5.11.3 Extinguishing Systems. 5.12 Postrelease Mitigation. 5.12.1 Reactive Release Countermeasures. 5.12.2 Reactive Chemicals Personal Protective Equipment. 5.12.3 Reactive Chemicals Emergency Response. 5.13 Hazard Reviews. 5.13.1 Hazard Severity Categories. 5.13.2 Reactive Chemicals Hazard Reviews. 5.14 Codes and Standards. References. 6. Process Safety Management of Reactive Material Facilities. 6.1 Accountability: Objective and Goals. 6.2 Process Knowledge and Documentation. 6.3 Capital Project Review and Design Procedures. 6.4 Process Risk Management. 6.5 Management of Change. 6.6 Process and Equipment Integrity. 6.7 Human Factors. 6.8 Personnel Training and Performance. 6.9 Incident Investigation. 6.10 Standards, Codes, and Regulations. 6.11 Audits and Corrective Actions. 6.12 Enhancement of Process Safety Knowledge. 6.13 Other Elements Required by Regulatory Authorities. Bibliography. References. 7. Specific Design Considerations. 7.1 Polymerizable Materials: Acrylic Acid. 7.2 Polymerizable Materials: Styrene. 7.3 Organic Peroxides. 7.4 Organic Peroxides: Dibenzoyl Peroxide. 7.5 Organic Peroxides: MEK Peroxide. 7.6 Temperature-Sensitive Materials: Ethylene Oxide. 7.7 Pyrophoric Materials: Aluminum Alkyls. 7.8 Peroxide Formers: 1,3-Butadiene. 7.9 Water-Reactive Materials: Sodium. 7.10 Water-Reactive Materials: Chlorosulfonic Acid. References. Appendix A. Reactive Chemicals Literature Sources. Procedures for Hazard Evaluation and Testing. Accident and Loss Prevention. Data Sources and Compilations. Material Safety Data Sheets. Computerized On-line Databases. Educational and Training Materials. Appendix B. Industry Practice Survey Results. Glossary. Index.

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Book SynopsisThis is a collection of formulas devoted entirely to products that serve as cleaning agents for personal hygiene, household uses, and industrial maintenance. All formulas are systematically presented first by application and then classified by their chemical constituents. The formulas include instructions for preparation. Because of the broad spectrum of cleaning agents included in the book it will be valuable to both the industrial and home chemists with formulas geared to the professional and the hobbyist.

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