Cellular and molecular neuroscience Books
Profile Books Ltd The Story of the Brain in 10½ Cells
Book SynopsisThere are more than 100 billion brain cells in our heads, and every single one represents a fragment of thought and feeling. Each cell possesses a mysterious beauty, with branching, intricate patterns like shattered glass. Richard Wingate has been scrutinising them for decades, yet he is still gripped by the myriad of forms when he looks down the microscope. With absorbing lyricism and clarity, Wingate shows how each type of cell possesses its own personality and history, illustrating a milestone of scientific discovery and illuminating the stories of pioneering scientists like Santiago Ramon y Cajal and Francis Crick, and capturing their own fascinating shapes and patterns. Discover the ethereal world of the brain with this elegant little book - and find out how we all think and feel.Trade ReviewA lyrical love letter to the art and science of neuroanatomy. In elegant, evocative prose, Wingate tells the very human tales of the early explorers of the nervous system, highlighting the joy of discovery and the insights that can be had if you just really look. -- Kevin J. Mitchell, Ph.D., Associate Professor of Genetics and Neuroscience, Trinity College DublinFew books [...] convey so succinctly the combination of care, determination, obsessiveness and imagination that scientists in a field such as neuroscience require to make an advance in knowledge ... A short and exhilarating book * The Oldie *This book is a wonderful description of the cells that make up the brain and how the remarkable organ works ... The author conveys his passion for neuroscience in this volume, which will leave the reader enthralled and wanting more. -- Dr Arpan K Banerjee * Hektoen International *Beautiful, mysterious, and intricate * The Idler *
£13.49
Springer-Verlag New York Inc. Receptor and Ion Channel Detection in the Brain
Book SynopsisThis second edition volume expands on the previous edition with new information on the latest developments in the field, and the most up-to-date representative methodological information on molecular neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters in this book are organized into three parts. Part One discusses molecular immunological and pharmacological aspects related to the detection of receptors and ion channels in the brain. Part Two explores the neuroanatomical techniques devoted to localizing receptors and ion channels within the central nervous system. Part Three looks at functional approaches devoted to ascertaining receptors and ion channels functionality in the brain. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and coTable of ContentsSeries Preface…Preface…Table of Contents…Contributing Authors…Part I Molecular Techniques1. Production of High-Quality Antibodies for the Study of Receptors and Ion ChannelsMasahiko Watanabe2. Co-Immunoprecipitation from BrainXavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe, Víctor Fernández-Dueñas, and Francisco Ciruela3. Sub-Synaptic Membrane FractionationPaula M. Canas and Rodrigo A. Cunha4. Quantitative Analysis of Cell Surface Expressed, Intracellular, and Internalized Neurotransmitter Receptor Populations in Brain Slices Using Biotinylation Elek Molnár5. Single Nanoparticle Tracking of Surface Ion Channels and Receptors in Brain CellsJuan Varela, Julien Dupuis, François Maingret, and Laurent Groc 6. Radioligand Binding Detection of Receptors in Brain MembranesFuencisla Pilar-Cuéllar, Emilio Garro-Martínez, Elena Castro, and Álvaro Díaz7. Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the BrainMarkus U. Ehrengruber and Kenneth Lundstrom8. Time Resolved-Fluorescence Resonance Energy Transfer using Fluorescent Ligands to Study Native G Protein-Coupled Receptor Heteromerization in Brain Víctor Fernández-Dueñas, Thierry Durroux, and Francisco Ciruela9. Study of GPCR Homo and Heteroreceptor Complexes in Specific Neuronal Cell Populations using the In Situ Proximity Ligation AssayManuel Narváez, Minerva Crespo-Ramírez, Ramón Fores-Pons, Mariana Pita-Rodríguez, Francisco Ciruela, Malgorzata Filip, Sarah Beggiato, Luca Ferraro, Sergio Tanganelli, Patrizia Ambrogini, Miguel Perezde la Mora, Kjell Fuxe, and Dasiel O. Borroto-Escuela10. Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers in Human Post-Mortem BrainMarta Valle-León, Víctor Fernández-Dueñas, and Francisco CiruelaPart II Neuroanatomical Techniques11. Fluorescent In Situ Hybridization for Sensitive and Specific Labeling Miwako Yamasaki and Masahiko Watanabe12. Autoradiographic Visualization of G Protein-Coupled Receptors in Brain Rebeca Vidal, Fuencisla Pilar-Cuellar, Álvaro Díaz, and Elena Castro13. Localization of Neurotransmitter Receptor and Ion Channel Proteins in Unfixed Brains using In Situ ImmunoblottingElek Molnár14. Immunohistochemistry for Ion Channels and their Interacting Molecules: Tips for Improving Antibody AccessibilityKohtarou Konno and Masahiko Watanabe15. Localization of GFP-Tagged Proteins at the Electron MicroscopeSara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil, J.S. Lee, M.V. Nachury, and José Manuel García-Verdugo16. Tyramide Signal Amplificación for Immunoelectron MicroscopyMaría José Ulloa-Navas, Patricia García-Tárraga, Susana González-Granero, Pedro Pérez-Borreda, Vicente Herranz-Pérez, and José Manuel García-Verdugo17. Pre-Embedding Methods for the Localization of Receptors and Ion ChannelsRafael Luján18. Post-Embedding Immunohistochemistry in the Localization of Receptors and Ion ChannelsRafael Luján and Masahiko Watanabe19. High-Resolution Localization and Quantitation of Membrane Proteins by SDS-Digested Freeze-Fracture Replica Labeling (SDS-FRL)Walter A. Kaufmann, David Kleindienst, Harumi Harada, and Ryuichi Shigemoto20. Pre-Embedding Immunostaining of Brain Tissue and Three-Dimensional Imaging with FIB-SEMMarta Turégano-López, José Rodrigo-Rodríguez, Lidia Alonso-Nanclares, Juncal González-Soriano, Javier DeFelipe, and Ángel Merchán-Pérez21. Application of Virus Vectors for Anterograde Tract-Tracing and Single-Neuron Labeling Studies Hiroyuki Hioki, Hisashi Nakamura, and Takahiro Furuta22. Efficient Labeling of Neurons and Identification of Postsynaptic Sites Using Adeno-Associated Virus VectorMegumu Takahashi, Yoko Ishida, Naoya Kataoka, Kazuhiro Nakamura, and Hiroyuki Hioki23. Analysis of Synaptic Connections at the Electron Microscopic Level using Sindbis Virus Vectors Takahiro Furuta, Keiko Okamoto-Furuta, and Hiroyuki Hioki24. Morphological and Neurochemical Characterization of Electrophysiologically Identified CellsYoshiyuki KubotaPart III Functional Techniques25. Using Electrophysiology to Study Synaptic and Extrasynaptic Ionotropic Receptors in Hippocampal NeuronsIan D. Coombs and David Soto26. Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium ChannelsNorbert Weiss and Michel De Waard27. Electrophysiological Recordings in Behaving AnimalsAgnès Gruart and José M. Delgado-García28. Voltammetry in Behaving AnimalsKendra D. Bunner and George V. Rebec29. In Vivo Brain Microdialysis of MonoaminesJorge E. Ortega, Blanca Pérez-Palomar, J. Javier Meana, and Luis F. Callado30. Optical Control of Brain Receptors using Photoactive Drug in Behaving AnimalsMarc López-Cano, Víctor Fernández-Dueñas, and Francisco Ciruela31. Dynamic Recording of Membrane Potential from Hippocampal Neurons by Using a Fluorescence Resonance Energy Transfer-Based Voltage Biosensor Víctor Fernández-Dueñas, Xavier Morató, Thomas Knöpfel, and Francisco CiruelaChapter 32. Monitoring GPCR-Mediated cAMP Accumulation in Rat Striatal SynaptosomesJaume Taura, Víctor Fernández-Dueñas, and Francisco Ciruela33. GPCR-Mediated MAPK/ERK Cascade Activation in Mouse Striatal SlicesMaricel Gómez-Soler, Víctor Fernández-Dueñas, and Francisco CiruelaSubject Index List…
£237.49
New India Publishing Agency Molecular Markers and Plant Biotechnology
Book Synopsis
£83.90
New India Publishing Agency Molecular Markers and Plant Biotechnology
Book SynopsisThe book entitled "Molecular Markers and Plant Biotechnology" is an exclusive collection of molecular marker based techniques narrated in 40 s through 578 along with figures makes it essential for biotechnology people. To supplement the practical working the relevant equipments have been described. Laboratory safety rules placed in the beginning is a wise task. Appendices include basic calculations; basic principles in preparation of reagents, abbreviations and glossary show the carefulness while preparing this text. This is an unavoidable text for biotechnology laboratory and class."Table of Contents1. Milestones in DNA history and biotechnology. 2. Equipments required in a molecular marker laboratory. 3. Isolation, purification and quantification of genomic DNA from plants. 4. Isolation, purification and quantification of RNA from plants. 5. Electrophoresis. 6. Molecular weight markers for gel electrophoresis. 7. Polycrylamide Gel Electrophoresis PAGE. 8. Gel electrophoresis of protein. 9. Isoenzyme. 10. Extraction of DNA fragments from Agarose Gel. 11. Why molecular markers? 12. Restriction enzyme digestion of DNA and its Agarose Gel Electrophoresis. 13. Thermocyclers PCR Machines. 14. Modifications of basic PCR technique. 15. Random amplified polymorphic DNA RAPD. 16. Restriction Fragment Length Polymorphism RFLP. 17. Amplified Fragment Length Polymorphism AFLP. 18. Simple sequence repeats Microsatellite. 19. Variable number of Tandem repeats Minisatellite. 20. Inter Simple Sequence Repeat ISSR. 21. Sequence Characterized Amplified Region SCAR. 22. Cleaved Amplified Polymorphic Sequence CAPS. 23. Single-Strand Conformation Polymorphism SSCP. 24. Retrotransposon-based markers S-SAP, IRAP, REMAP, RBIP, RGAS, SNP. 25. Silver staining. 26. Basics of marker assisted selection in crop plants. 27. Mapping populations. 28. QTL mapping. 29. Southern blotting. 30. Western blotting. 31. Northern blotting. 32. Eastern blotting. 33. DNA sequencing and designing of primers. 34. Gene transfer to plant. 35. Fluorescent in Situ Hybridization FISH. 36. Genotypic Barcoding. 37. mtDNA. 38. MicroRNA. 39. Graphical approach to calculate molecular weights of DNA/protein fragments. 40. Calculation of similarity index values and construction of Dendrogram using NTSYSpc 2.0.
£151.24
