Description
Book SynopsisThis detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research.
Table of Contents1. A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment
Magdalena Sutcliffe and Madeline A. Lancaster
2. Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells
Eva Tomaskovic-Crook and Jeremy M. Crook
3. Organoid Assay of Human Cancer Stem Cells
Yohei Shimono, Piero Dalerba, Junko Mukohyama, Taichi Isobe, Darius Johnston, and Akira Suzuki
4. Construction of Thymus Organoids from Decellularized Thymus Scaffolds
Asako Tajima, Isha Pradhan, Xuehui Geng, Massimo Trucco, and Yong Fan
5. Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres
Robert E. Hynds, Colin R. Butler, Sam M. Janes, and Adam Giangreco
6. Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells
Mahboobe Ghaedi and Laura E. Niklason
7. Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix
Hiroko Hisha and Hiroo Ueno
8. Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension
Valentina Benedetti, Valerio Brizi, and Christodoulos Xinaris
9. Efficient Culture of Intestinal Organoids with Blebbistatin
Zhen Qi and Ye-Guang Chen
10. Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion
Dustin J. Flanagan, Renate H.M. Schwab, Bang M. Tran, Toby J. Phesse, and Elizabeth Vincan
11. The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine
Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, and Maja Grabacka
12. New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice
Toshio Takahashi
13. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System
Jessica L. Forbester, Nicholas Hannan, Ludovic Vallier, and Gordon Dougan
14. Murine Colonic Organoid Culture System and Downstream Assay Applications
Yang-Yi Fan, Laurie A. Davidson, and Robert S. Chapkin
15. Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions
Giulia Nigro, Melissa Hanson, Cindy Fevre, Marc Lecuit, and Philippe J. Sansonetti
16. The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism
Diana Li, Hongli Dong, and Alison B. Kohan
17. Oncogenic Transformation of Human-Derived Gastric Organoids
Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, and Yana Zavros
18. Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation
Yun Chen, Chuan Li, Sheng-Hong Tseng, and Ya-Hui Tsai
19. Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections
Winnie Y. Zou, Sarah E. Blutt, Sue E. Crawford, Khalil Ettayebi, Xi-Lei Zeng, Kapil Saxena, Sasirekha Ramani, Umesh Karandikar, Nicholas C. Zachos, and Mary K. Estes
20. Study Bacterial-Host Interactions Using Intestinal Organoids
Yong-guo Zhang and Jun Sun
21. Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering
Megan K. Eldred, Leila Muresan, and William A. Harris
22. Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo
Kai Tong, Mahendra Wagle, and Su Guo
23. Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells
Yoni Baert, Charlotte Rombaut, and Ellen Goossens
24. Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids
Tiziana A.L. Brevini, Elena F.M. Manzoni, Sergio Ledda, and Fulvio Gandolfi
25. Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly
Yuqing Zhu, Vahid Serpooshan, Sean Wu, Utkan Demirci, Pu Chen, and Sinan Güven
26. Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying
Mozhdeh Imaninezhad, Era Jain, and Silviya Petrova Zustiak
27. Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues
Soojung Hahn and Jongman Yoo
28. Drug Sensitivity Assays of Human Cancer Organoid Cultures
Hayley E. Francies, Andrew Barthorpe, Anne McLaren-Douglas, William J. Barendt, and Mathew J. Garnett
