Description
Book SynopsisThis detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful
Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.
Table of Contents1. Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy
Sharol Schmidt-Marcec, Austin Ross, and Andrei Smertenko
2. Actin: Static and Dynamic Studies
Huaqiang Ruan, Sha Zhang, Yi Zhang, and Haiyun Ren
3. 3D Visualization of Microtubules in Epidermal Pavement Cells
Amir J. Bidhendi, Bara Altartouri, and Anja Geitmann
4. Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells
Sandra Klemm, Jonas Buhl, Birgit Möller, and Katharina Bürstenbinder
5. Single Cell Confinement Methods to Study Plant Cytoskeleton
Pauline Durand-Smet, Antoine Chevallier, Léia Colin, Alice Malivert, Isaty Melogno, and Olivier Hamant
6. Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures
Eduard Belausov, Vikas Dwivedi, Sela Yechezkel, Sefi Bar-Sinai, and Einat Sadot
7. Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography
Janice Pennington and Marisa S. Otegui
8. Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana)
Yuh-Ru Julie Lee, Calvin H. Huang, and Bo Liu
9. Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis
Sabine Müller
10. Expansion Microscopy of Plant Cells (PlantExM)
Timothy J. Hawkins, Joanne L. Robson, Bethany Cole, and Simon J. Bush
11. Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens
Mari W. Yoshida and Elena Kozgunova
12. Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens
Yuji Hiwatashi and Takashi Murata
13. Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools
Fatima Cvrčková and Radek Bezvoda
14. Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata
Zoe Barr and Jens Tilsner
15. Studying Nuclear Dynamics in Response to Actin Disruption In Planta
Joseph F. McKenna and Katja Graumann
16. Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination
Lucie Riglet and Isabelle Fobis-Loisy
17. Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes
Lei Zhu and Ying Fu
18. Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study
Teresa Braga, Fernando Vaz Dias, Marta Fratini, Susana Serrazina, Ingo Heilmann, and Rui Malhó
19. Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis
Liru Dou, Xiangfeng Wang, and Tonglin Mao
20. Imaging of Cortical Microtubules in Plants under Salt Stress
Shuwei Wang, Liyuan Xu, Changjiang Li, Lei Zhu, Ying Fu, and Yan Guo
21. Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli
Bingxiao Wang, Minxia Zou, Qing Pan, and Jiejie Li
22. Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation
Amber J. Connerton, Stefan Sassmann, and Michael J. Deeks
23. Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes
Qiaonan Lu, Xiaonan Liu, Xiaolu Qu, and Shanjin Huang
24. Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings
Felix Ruhnow, Staffan Persson, and René Schneider
25. Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging
Guangda Wang, Yanjun Yu, and Zhaosheng Kong
26. Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells
Xie Dang, Lilan Zhu, Huibo Ren, and Deshu Lin
27. Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato
Zhijing Xu, Xiaolu Qu, Shuang Wu, and Pengwei Wang
28. Light Microscopy Technologies and the Plant Cytoskeleton
Timothy J. Hawkins
29. Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton
Patrick Duckney and Patrick J. Hussey
