Description
Book SynopsisThis guide provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of haemopoietic cells. Covering a range of human and mouse cell types, and featuring step-by-step laboratory procedures, it gives detailed instructions for reagent preparation.
Table of ContentsPartial table of contents:
PRIMITIVE PROGENITOR ASSAYS.
Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher).
Assay for Murine Blast Cell Colonies (N. Katayama & M.Ogawa).
Serum-Deprived Cultures of Primary Hematopoietic Cells (G.Migliaccio & A. Migliaccio).
COLONY-FORMING ASSAYS FOR MALIGNANT CELLS.
Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, etal.).
LONG-TERM ASSAYS AND IMMORTALIZATION.
Long-Term Culture of Human Myeloid Cells (H. Sutherland & C.Eaves).
Immortalization of Hematopoietic Cells (C. MacDonald).
PURIFICATION OF PROGENITOR CELLS.
Purification of Murine Granulocyte-Macrophage Progenitor Cells(CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper &H. Broxmeyer).
OTHER SYSTEMS AND ASSAYS.
Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins& R. Fleischman).
List of Suppliers.
Index.
