Description
Book SynopsisThis third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge,
Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.
Table of Contents1. From olive oil emulsions to COVID-19 Vaccines - Liposomes Came First
Volkmar Weissig
2 Preparation of DRV liposomes
Sophia G. Antimisiaris
3 Preparation of small unilamellar vesicles using detergent dialysis method
Qingyue Zhong and Hongwei Zhang
4 Thin-film hydration followed by extrusion method for liposome preparation
Hongwei Zhang
5 Ethanol injection method for liposome preparation
Guangsheng Du and Xun Sun
6 Preparation of giant vesicles with mixed single-tail and double-tail lipids
Lauren A Lowe and Anna Wang
7 Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing
Jung Yeon Han, Zhu Chen, and Don L. DeVoe
8 Preparation of doxorubicin liposomes by remote loading method
Jian Chen
9 Magnetic thermosensitive liposomes loaded with Doxorubicin
Mohamad Alawak, Alice Abu Dayyih, Ibrahim Awak, Bernd Gutberlet, Konrad Engelhardt, and Udo Bakowsky
10 Preparation and physical characterization of DNA binding cationic liposomes
Vaibhav Saxena
11 Tunable pH sensitive lipoplexes
Helene Dhotel, Michel Bessodes and Nathalie Mignet
12 Solid Lipid Nanoparticles for Drug Delivery
Wei-Chung Luo and Xiuling Lu
13 Stable Discoidal Bicelles – Formulation, Characterization and Functions
Ying Liu, Yan Xia, Armin Tahmasbi Rad, Wafa Aresh, Justin M. Fang, and Mu-Ping Nieh
14 The post-insertion method for the preparation of PEGylated liposomes
Sherif E. Emam, Nehal E. Elsadek, Taro Shimizua, and Tatsuhiro Ishidaa
15 Click chemistry for liposome surface modification
Maria Vittoria Spanedda, Marcella De Giorgi, Béatrice Heurtault, Antoine Kichler, Line Bourel-Bonnet and Benoît Frisch
16 Surface Modification of Liposomes Using Folic Acid
Mengran Guo, Zhongshan He, Xi He and Xiangrong Song
17 Preparation and Characterization of Trastuzumab Fab conjugated liposomes (immunoliposomes)
Yuhong Xu
18 Pyrophosphorylated cholesterol modified bone-targeting liposomes formulation procedure
Yanzhi Liu, Zhenshan Jia, Luoyang Ma, and Dong Wang
19 Method of simultaneous analysis of liposome components using HPTLC/FID
Sophia Hatziantoniou and Costas Demetzos
20 HPLC-MS/MS Method for Identification and Quantification of Lipids in Liposomes
Yujie Shi and Xiaona Li
21 DPH probe method for liposome-membrane fluidity determination
Wei He
22 Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy
Anand S. Ubhe
23 Visualization and characterization of liposomes by Atomic Force Microscopy (AFM)
Konrad Engelhardt, Eduard Preis and Udo Bakowsky
24 Determination of the sub-cellular distribution of fluorescently labeled liposomes using confocal microscopy
Melani A. Solomon
25 Liposome biodistribution via europium complexes
Nathalie Mignet and Daniel Scherman
26 Quantification of a fluorescent lipid DOPE-NBD by an HPLC method in biological tissue: application to study liposome’s uptake by human placenta
Louise Fliedel, Nathalie Mignet, Thierry Fournier, Karine Andrieux, and Khair Alhareth
