Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium.- SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures.- A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells.- Expansion Microscopy of Ciliary Proteins.- Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes.- Structural Analysis of Sperm Centrioles Using N-STORM.- A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium.- Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets.- Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging.- Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells.- Proximity Mapping of C
Table of ContentsPreface…
Table of Contents…
Contributing Authors…
1. Combination of CRISPR-Cas9-RNP and Single-Cell RNAseq to Identify Cell State-Specific FOXJ1 Functions in the Human Airway Epithelium
Laure-Emmanuelle Zaragosi, Alizé Gouleau, Margot Delin, Kevin Lebrigand, Marei-Jeanne Arguel, Cedric Girard-Riboulleau, Geraldine Rios, Elisa Redman, Magali Plaisant, Rainer Waldmann, Virginie Magnone, Brice Marcet, Pascal Barbry, and Gilles Ponzio
2. SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures
Maria Victoria Humbert, Christopher J. McCormick, and Cosma Mirella Spalluto
3. A Chemically Inducible Organelle Rerouting Assay to Probe Primary Cilium Assembly, Maintenance, and Disassembly in Cultured Cells
F. Basak Turan, M. Erdem Ercan, and Elif Nur Firat-Karalar
4. Expansion Microscopy of Ciliary Proteins
Sohyeon Park and Xiaoyu Shi
5. Immunolabel-First-Expand-Later Expansion Microscopy Approach using Stable STED Dyes
Dong Kong, Delgermaa Luvsanjav, and Jadranka Loncarek
6. Structural Analysis of Sperm Centrioles Using N-STORM
Abigail Royfman, Sushil Khanal, and Tomer Avidor-Reiss
7. A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium
Nobuhiro Morone, Maria Guerra Martin, Patricia Goggin, Kirk Czymmek, Vito Mennella, and Jaime Llodra Gonzalez
8. Airway Cells 3D Reconstruction via Manual and Machine-Learning Aided Segmentation of Volume EM Datasets
Aaran Vijayakumaran, Analle Abuammar, Odara Medagedara, Kedar Narayan, and Vito Mennella
9. Endogenous Tagging of Ciliary Genes in Human RPE1 Cells for Live-Cell Imaging
Stefanie Kuhns, Alice Dupont Juhl, Zeinab Anvarian, Daniel Wüstner, Lotte B. Pedersen, and Jens S. Andersen
10. Live-Imaging Centriole Amplification in Mouse Brain Multiciliated Cells
Amélie-Rose Boudjema, Adel Al Jord, Anne-Iris Lemaître, Marion Faucourt, Nathalie Delgehyr, Nathalie Spassky, and Alice Meunier
11. Proximity Mapping of Ciliary Proteins by BioID
Melissa Iazzi, Jonathan St-Germain, Saujanya Acharya, Brian Raught, and Gagan D. Gupta
12. Affinity Purification of Intraflagellar Transport (IFT) Proteins in Mice using Endogenous Streptavidin/FLAG Tags
Tina Beyer, Tiago Martins, Jeshmi Jeyabalan Srikaran, Marian Seda, Emma Peskett, Franziska Klose, Katrin Junger, Philip L. Beales, Marius Ueffing, Karsten Boldt, and Dagan Jenkins
13. Primary Human Nasal Epithelial Cell Culture
Mystica Terrance, Tarini Gunawardena, Hong Ouyang, Julie Avolio, Wenming Duan, Sowmya Thanikachalam, and Theo J. Moraes
14. BMI1 Transduction of Human Airway Epithelial Cells for Expansion of Proliferation and Differentiation
Ruhina Maeshima, Amy Jacobs, Melis T. Dalbay, and Stephen L. Hart
15. High Speed Video Microscopy of Ependymal Cilia in Brain Organotypic and Cell Culture Models
William J. Dawes, Oriane Grant, Sam C. Reitemeier, Sarah Tetlow, Dani Lee, Robert A. Hirst, and Christopher O’Callaghan
16. Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods
Erika Causa, Ricardo Fradique, and Pietro Cicuta
17. Mucociliary Transport Device Construction and Application to Study Mucociliary Clearance
Patrick R. Sears and Lawrence E. Ostrowski
Subject Index List…
