Biochemistry Books
de Gruyter Fortschritte Auf Dem Gebiet Der Untersuchungen
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£134.09
de Gruyter Principles of Peptide Synthesis
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£117.50
de Gruyter Die Biochemischen Grundlagen Des
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£134.09
de Gruyter Makromoleküle ALS Träger Von Lebensprozessen
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£134.09
de Gruyter Medicinal Implications in Cytochrome P450
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£111.62
De Gruyter Relationships Between Structure and Function of
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£123.33
de Gruyter Über Sekundäre Pflanzenstoffe
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£134.09
de Gruyter Hauptgruppe 5
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£134.09
de Gruyter Werkstofferhaltung Und Umweltschutz
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£134.09
Logos Verlag Berlin GmbH Enzymatic Stabilization and Formation of Food
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£51.07
Almuzara Historia del Arsenico
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£19.47
World Health Organization Strontium and Strontium Compounds
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£23.31
IARC Trichloroethylene, tetrachloroethylene and some
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£59.03
McGraw-Hill Education - Europe Lab Manual for General Organic Biochemistry
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£123.81
Princeton University Press Aquatic Photosynthesis
Book SynopsisA comprehensive guide to understanding the evolution and ecology of photosynthesis in aquatic environments. This second edition describes how one of the most fundamental metabolic processes evolved and transformed the surface chemistry of the Earth. It focuses on biochemical and biophysical advances and the molecular biological techniques.Trade Review"Despite the ubiquity and critical importance of this topic to the study of aquatic ecology, there are very few books dedicated primarily to aquatic photosynthesis, and none cover the subject matter with comparable elegance or continuity from the molecule to the ecosystem as does this one, authored by two of the leading researchers in the subject... [I]t is a pleasure to have this important volume back in print after a hiatus of several years. No other text covers the subject as lucidly or completely as this one, and that makes it an extremely valuable pedagogical reference. It will be a welcome addition to any library or personal collection."--Richard C. Zimmerman, Limnology and Oceanography Bulletin "Indeed a magnificent book that will, perhaps, be welcomed as one of the best and the most useful ones for all biologists and oceanographers... No other text covers the subject as lucidly or completely as this one, and that makes it an extremely valuable pedagogical reference. It will be a welcome addition to any library or personal collection."--Richard C. Zimmerman, Current Engineering PracticeTable of ContentsPreface to the Second Edition ix Preface to the First Edition xi Chapter 1: An Introduction to Photosynthesis in Aquatic Systems 1 Chapter 2: Light Absorption and Energy Transfer in the Photosynthetic Apparatus 44 Chapter 3: The Photosynthetic Light Reactions 81 Chapter 4: Photosynthetic Electron Transport and Photophosphorylation 118 Chapter 5: Carbon Acquisition and Assimilation 156 Chapter 6: The Molecular Structure of the Photosynthetic Apparatus 201 Chapter 7: Photosynthesis in Continuous Light 237 Chapter 8: Making Cells 278 Chapter 9: Photosynthesis and Primary Production in Nature 319 Chapter 10: Aquatic Photosynthesis in Biogeochemical Cycles 364 References 411 Index 465
£999.99
Springer Principles and Practices of Winemaking
Book Synopsis1 Introduction.- 2 Viticulture for Winemakers.- 3 Preparation of Musts and Juice.- 4 Yeast and Biochemistry of Ethanol Fermentation.- 5 Red and White Table Wines.- 6 Malolactic Fermentation.- 7 The Fining and Clarification of Wines.- 8 The Physical and Chemical Stability of Wine.- 9 Microbiological Spoilage of Wine and Its Control.- 10 The Maturation and Aging of Wines.- 11 The Bottling and Storage of Wines.- 12 The Role of Sulfur Dioxide in Wine.- 13 Must, Juice, and Wine Transfer Methods.- 14 Heating and Cooling Applications.- 15 Juice and Wine Acidity.- 16 Preparation, Analysis, and Evaluation of Experimental Wines.- Appendices.Table of ContentsPreface. Introduction. Viticulture for Winemakers. Preparation of Musts and Juice. Yeast and Biochemistry of Ethanol Fermentation. Red and White Table Wines. Malodactic Fermentation. The Fining and Clarification of Wines. The Physical and Chemical Stability of Wine. Microbiological Spoilage of Wine and its Control. The Maturation and Aging of Wine. The Bottling and Storage of Wines. The Role of Sulfur Dioxide in Wine. Must, Juice and Wine Transfer Methods. Heating and Cooling Applications. Juice and Wine Acidity. Preparation. Analysis and Evaluation of Experimental Wines. Appendixes. Glossary
£999.99
Springer-Verlag New York Inc. The Ubiquitin Code
Book SynopsisThis volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle oTable of ContentsPart I: Chain Diversity 1 Thioester and oxyester linkages in the ubiquitin system Alba Ferri-Blazquez, Ernst Jarosch and Thomas Sommer 2 Getting to the root of branched ubiquitin chains: A review of current methods and functions Anita Waltho, Thomas Sommer Part II: Ub and UbL Chemical Tools 3 Chemical synthesis of non-hydrolysable Ubiquitin(-like) hybrid chains David A. Pérez Berrocal, Gerbrand J. van der Heden van Noort and Monique P. C. Mulder 4 Total Linear Chemical Synthesis of LC3A and LC3B Yara Huppelschoten, Jens Buchardt, Thomas Eiland Nielsen and Gerbrand J. van der Heden van Noort Part III: Methods to study Conjugating enzymes (E3s) 5 A microbead-based flow cytometry assay to assess the activity of Ubiquitin and Ubiquitin-like conjugating enzymes Clara Recasens-Zorzo, Pierre Gâtel, Frédérique Brockly and Guillaume Bossis 6 Monitoring HECT ubiquitination activity in vitro Vincenzo Taibi, Simona Polo and Elena Maspero1 Part IV: Methods to study DUBs 7 RNAi-based screening for the identification of specific substrate-deubiquitinase pairs Nagore Elu, Natalia Presa and Ugo Mayor 8 Strategies for monitoring ‘ubiquitin C-terminal hydrolase 1’ (Yuh1) activity Shahaf Saad, Eden Berda, Yuval Klein, Suha Issa and Elah Pick Part V: Methods to identify new targets and regulators 9 Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome using Chain-Specific Nanobodies Maria Gonzalez-Santamarta, Laurie Ceccato, Ana Sofia Carvalho, Jean-Christophe Rain, Rune Matthiesen and Manuel S. Rodriguez 10 A mass spectrometry-based strategy for mapping modification sites for the Ubiquitin-like modifier NEDD8 Caio A.B. Oliveira, Ekaterina Isaakova, Petra Beli and Dimitris P. Xirodimas 11 Isolation of the hepatic ubiquitome/NEDDylome by streptavidin pull-down assay in the biotinylated ubiquitin (bioUb)/biotinylated NEDD8 (bioNEDD8) transgenic mice Marina Serrano-Maciá, Teresa Cardoso Delgado and María Luz Martínez-Chantar 12 The Lambda display technology: a useful tool for the identification of ubiquitin and ubiquitin like-binding domains Elena Santonico 13 SUMO-ID: a strategy for the identification of SUMO-dependent proximal interactors Orhi Barroso-Gomila, Ugo Mayor, Rosa Barrio and James D. Sutherland 14 Analysis of ATG8 family members using LC3-Interacting Regions (LIR)-based molecular traps Grégoire Quinet, Pierre Génin, Naima Belgareh-Touzé, Oznur Ozturk, Robert Weil, Mickael M. Cohen, Renaud Legouis and Manuel S. Rodriguez 15 A computational tool for analysis of mass spectrometry data of ubiquitin enriched samples Rune Matthiesen, Manuel S. Rodriguez, and Ana Sofia Carvalho Part VI: to measure proteasome activity 16 In-plate and in-gel assays for the assessment of proteasome activity in Caenorhabditis elegans Eleni Panagiotidou, Anna Gioran and Niki Chondrogianni 17 Isolation of Proteasome-Trapped Peptides (PTPs) for degradome analysis Indrajit Sahu, Manisha Priyadarsini Sahoo, Oded Kleifeld and Michael H. Glickman
£143.99
Springer-Verlag New York Inc. Heterologous Expression of Membrane Proteins
Book SynopsisThis detailed volume explores protocols for the production of membrane proteins in a panel of heterologous organisms for structural studies. Beginning with techniques using E. coli as a host for the overproduction and purification of membrane proteins, the book continues with chapters covering mammalian membrane protein production in yeast, insect cells, mammalian cells, as well as using virus like particles and acellular systems. Additionally, new detergents and alternatives to detergents allowing membrane protein purification for structural analyses are described. The book closes with a chapter exploring the use of microscale thermophoresis (MST) to evaluate the binding activity of heterologously expressed proteins directly in crude membrane extracts. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryTable of Contents1. Solubilization and Purification of a5b1 Integrin from Rat Liver for Reconstitution into Nanodiscs Estelle Dransart, Aurélie Di Cicco, Ahmed El Marjou, Daniel Lévy, Staffan Johansson, Ludger Johannes, and Massiullah Shafaq-Zadah 2. Membrane Proteins Production in Escherichia coli: Protocols and Rules Jordi Royes, Pauline Talbot, Christel Le Bon, Karine Moncoq, Marc Uzan, Francesca Zito, and Bruno Miroux 3. Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly Margot Di Cesare, Aissatou Maty Diagne, Benjamin Bourgey, Jean-Michel Jault, and Cédric Orelle 4. Heterologous Expression of Membrane Proteins in E. coli Peer Depping, María Monserrat Román Lara, Athanasios Kesidis, Roslyn M. Bill, Alice J. Rothnie, Douglas F. Browning, and Alan D. Goddard 5. Overexpression of the ABC Transporter BmrA within Intracellular Caveolæ in Escherichia coli Alexia Gobet, Christine Jaxel, Sandrine Magnard, Manuel Garrigos, Stéphane Orlowski, Nadège Jamin, Pierre Falson, and Vincent Chaptal 6. Production of Membrane Proteins in Pseudomonas stutzeri Hao Xie, Cornelia Muenke, Manuel Sommer, Sabine Buschmann, and Hartmut Michel 7. Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae Landon Haslem, Marina Brown, Xin A. Zhang, Jennifer M. Hays, and Franklin A. Hays 8. Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae Landon Haslem, Marina Brown, Xin A. Zhang, Jennifer M. Hays, and Franklin A. Hays 9. Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p Jorgaq Pata, Alexis Moreno, Sandrine Magnard, Atanu Banerjee, Rajendra Prasad, and Pierre Falson 10. Membrane Protein Production in the Yeast, P. pastoris Hoor Ayub, Michelle Clare, Luke Broadbent, John Simms, Alan D. Goddard, Alice J. Rothnie, and Roslyn M. Bill 11. Production and Preparation of Isotopically-Labelled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses Lina Barret, Tobias Schubeis, Valérie Kugler, Lucile Guyot, Guido Pintacuda, and Renaud Wagner 12. Membrane Protein Production in Insect Cells Afroditi Vaitsopoulou, Peer Depping, Roslyn M. Bill, Alan D. Goddard, and Alice J. Rothnie 13. Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies Pawel Krawinski and Martin Caffrey 14. Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies Ana S. Ramírez, Kamil Nosol, and Kaspar P. Locher 15. The Heterologous Expression and Purification of GPCRs Xiaoting Li, Ling Shen, Junlin Liu, Xiaoyan Liu, Zhi-Jie Liu, and Tian Hua 16. Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor Lukas Helfinger and Christopher G. Tate 17. Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-Like Particles Kathleen Aertgeerts, Thao T. Ho, and Yingzhou G. Yan 18. Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface Valeria Jaramillo-Martinez, Vadivel Ganapathy, and Ina L. Urbatsch 19. Tailor the Purification and Analysis of Membrane Proteins with Modular Detergents Leonhard H. Urner 20. Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers Valentina S. Dimitrova, Saemee Song, Alexandra Karagiaridi, Anika Marand, and Heather W. Pinkett 21. Detergent-Free Membrane Protein Purification Using SMA Polymer Luke Broadbent, Peer Depping, Alexis Lodé, Afroditi Vaitsopoulou, David Hardy, Hoor Ayub, James Mitchell-White, Ian D. Kerr, Alan D. Goddard, Roslyn M. Bill, and Alice J. Rothnie 22. Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies Simon Umbach, Volker Dötsch, and Frank Bernhard 23. Cell-Free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs Hoa Quynh Do and Michaela Jansen 24. Microscale Thermophoresis to Evaluate the Functionality of Heterologously Overexpressed Membrane Proteins in Membrane Preparations Sandra Kovachka, Pierre Soule, and Isabelle Mus-Veteau
£999.99
Springer-Verlag New York Inc. Clinical Applications of Mass Spectrometry in
Book SynopsisThis updated volume provides stepwise instructions for the analysis of numerous clinically important analytes by mass spectrometry. Mass spectrometry offers clinical laboratory scientists a number of advantages including increased sensitivity and specificity, multiple component analysis, and limited need for specialized reagents. These techniques are essential in laboratory fields including endocrinology, biochemical genetics, drug analysis, proteomics, and pathogen identification. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Clinical Applications of Mass Spectrometry in Biomolecular Analysis: Methods and Protocols, Second Edition is an ideal resource fTable of Contents 1. Introduction to Mass Spectrometry for Bimolecular Analysis in a Clinical Laboratory Y. Victoria Zhang and Uttam Garg 2. System Performance Monitoring in Clinical Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Shannon Haymond 3. Tandem Mass Spectrometry for the Analysis of Plasma/Serum Acylcarnitines for the Diagnosis of Certain Organic Acidurias and Fatty Acid Oxidation Disorders David Scott, C. Clinton Frazee III, Bryce Heese, and Uttam Garg 4. Quantification of Plasma S-Adenosylmethionine and S-Adenosylhomocysteine Using Liquid Chromatography Electrospray Tandem Mass Spectrometry Erland Arning, Brandi Wasek, and Teodoro Bottiglieri 5. Quantitation of Aldosterone in Serum or Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) J. Grace Van Der Gugten and Daniel T. Holmes 6. Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism Stephen M. Roper, Annette L. Weindel, and Dennis J. Dietzen 7. Quantification of Branched-Chain Amino Acids in Plasma by High-Performance Liquid Chromatography-Tandem Mass Spectrometry (LC- MS/MS) Hamed Piri-Moghadam, Alan Miller, Debra Pronger, Faye Vicente, Joel Charrow, Shannon Haymond, and David C. Lin 8. Quantitation of Butyrylcarnitine, Isobutyrylcarnitine, and Glutarylcarnitine in Urine Using Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry (UPLC-MS/MS) Judith A. Hobert, Stephen A. Brose, and Marzia Pasquali 9. Quantification of Free and Total Carnitine in Serum Using Liquid Chromatography Tandem Mass Spectrometry Serena Baird, C. Clint Frazee III, and Uttam Garg 10. Sensitive and Robust LC-MS/MS Analysis of Salivary Cortisol in Negative Mode Wayne B. Anderson, Putuma P. Gqamana, and Y. Victoria Zhang 11. Measurement of Urine Free Cortisol and Cortisone by LC-MS/MS Julie A. Ray, Erik Kish-Trier, and Lisa M. Johnson 12. Laboratory Diagnosis of Cerebral Creatine Deficiency Syndromes by Determining Creatine and Guanidinoacetate in Plasma and Urine Ning Liu and Qin Sun 13. Quantitation of Estradiol and Testosterone in Serum Using LC-MS/MS Ryan C. Schofield, Daniel Kirchoff, and Dean C. Carlow 14. Quantitation of Fatty Acids in Serum/Plasma and Red Blood Cells by Gas Chromatography-Negative Chemical Ionization-Mass Spectrometry Erik Kish-Trier and Tatiana Yuzyuk 15. Quantitation of γ-Aminobutyric Acid in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry Erland Arning, Brandi Wasek, and Teodoro Bottiglieri 16. A Simple, Fast, and Reliable LC-MS/MS Method for the Measurement of Homovanillic Acid and Vanillylmandelic Acid in Urine Specimens Vrajesh Pandya and Elizabeth L. Frank 17. Quantitation of Neuroblastoma Markers Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine by Gas Chromatography-Mass Spectrometry (GC/MS) Melissa Beals, Bheemraj Ramoo, C. Clinton Frazee III, and Uttam Garg 18. Quantification of 5-Hydroxyindoleacetic Acid in Urine by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry Heather A. Nelson and Elizabeth L. Frank 19. Quantitation of IgG Subclasses in Serum Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) J. Grace Van Der Gugten, Daniel T. Holmes, and Andre Mattman 20. Quantification of Insulin Analogs by Liquid Chromatography-High Resolution Mass Spectrometry Erica M. Fatica, Nicholas E. Larkey, and Ravinder J. Singh 21. Differentiation of Common IGF-1 Variants Using HRAM-MS COM Determination with Follow-Up MS/MS Verification Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh 22. Monitoring and Identifying Insulin-Like Growth Factor 1 Variants by Liquid Chromatography-High Resolution Mass Spectrometry in a Clinical Laboratory Ievgen Motorykin, Allison Li, and Zengru Wu 23. Quantitation of Lactate in Cerebrospinal Fluid Using Liquid Chromatography-Electrospray Tandem Mass Spectrometry Brandi Wasek and Erland Arning 24. Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry Hsuan-Chieh (Joyce) Liao and Hsiao-Jan Chen 25. Plasma Lysosphingolipid Biomarker Measurement by Liquid Chromatography-Tandem Mass Spectrometry Brandon B. Stauffer and Chunli Yu 26. Detection of 13C-Mannitol and Other Saccharides Using Tandem Mass Spectrometry for Evaluation of Intestinal Permeability, or Leaky Gut Nicholas E. Larkey, Erica M. Fatica, and Ravinder J. Singh 27. LC-MS/MS Method for High Throughput Analysis of Methylmalonic Acid in Serum, Plasma, and Urine: Method for Analyzing Isomers without Chromatographic Separation Mark M. Kushnir, Gordon J. Nelson, Elizabeth L. Frank, and Alan L. Rockwood 28. Quantitation of 5-Methyltetrahydrofolate in Cerebrospinal Fluid Using Liquid Chromatography Electrospray Tandem Mass Spectrometry Erland Arning, Brandi Wasek, and Teodoro Bottiglieri 29. Screening of Organic Acidurias by Gas Chromatography Mass Spectrometry (GC-MS) David Scott, C. Clinton Frazee III, and Uttam Garg 30. Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease and Gas Chromatography-Mass Spectrometry (GC/MS) Stanley F. Lo, Keely Pierzchalski, Velta Young, and William J. Rhead 31. Quantitative Organic Acids in Urine by Two-Dimensional Gas Chromatography-Time-of-Flight Mass Spectrometry (GCxGC-TOFMS) Erland Arning, Paula Ashcraft, Jeanna Bennett-Firmin, and Lawrence Sweetman 32. Quantification of Parathyroid Hormone and Its Fragments in Serum by Liquid Chromatography-High Resolution Mass Spectrometry Erica M. Fatica, Nicholas E. Larkey, and Ravinder J. Singh 33. High Sensitivity Measurement of Parathyroid Hormone Related Protein (PTHrP) in Plasma by LC-MS/MS Mark M. Kushnir and Alan L. Rockwood 34. Quantitation of Phenylalanine in Dried Blood Spot using Liquid Chromatography Tandem Mass Spectrometry for Monitoring of Patients with Phenylketonuria (PKU) Serena Baird, C. Clinton Frazee III, and Uttam Garg 35. An Optimized Procedure for Proteomic Analysis of Extracellular Vesicles Using In-Stage Tip Digestion and DIA LC-MS/MS: Application to Liquid Biopsy in Cancer Rajesh K. Soni, Lyssa Dimapanat, Manpreet S. Katari, and Alex J. Rai 36. High-Throughput Plasma Proteomic Profiling Rajesh Kumar Soni 37. Quantitation of Purine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Qin Sun 38. Quantitation of Pyrimidine in Urine by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry Ning Liu and Qin Sun 39. Quantitation of Renin Activity in Plasma Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) J. Grace Van Der Gugten and Daniel T. Holmes 40. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method for the Quantification Steroids Androstenedione, Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone Ada Munar, C. Clinton Frazee III, and Uttam Garg 41. A User-Friendly Sample Preparation Alternative for Manual and Automated LC-MS/MS Quantification of Testosterone Judy A. Stone, Dawn Francisco, Heather Tone, Joshua Akin, and Robert L. Fitzgerald 42. Quantitation of Thyroglobulin in Serum Using SISCAPA and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) J. Grace Van Der Gugten, Morty Razavi, and Daniel T. Holmes 43. Quantitation of Free Thyroxine by Equilibrium Dialysis and Liquid Chromatography-Tandem Mass Spectrometry Weijia (William) Wu, Michael J. McPhaul, and Zengru Wu 44. Quantification of Tryptophan, Indole, and Indoxyl Sulfate in Urine Using Liquid Chromatography-Tandem Mass Spectrometry Serena Baird, C. Clinton Frazee III, and Uttam Garg 45. Very Long-Chain Fatty Acids Quantification by Gas-Chromatography Mass Spectrometry Anna I. Scott 46. Quantification of Very Long-Chain and Branched-Chain Fatty Acids in Plasma by Liquid Chromatography-Tandem Mass Spectrometry Irene De Biase and Marzia Pasquali 47. High Throughput Analysis of 25-OH-Vitamin D2 and D3 Using Multi-Channel Liquid Chromatography-Tandem Mass Spectrometry Putuma P. Gqamana and Y. Victoria Zhang 48. Quantification of 25-Hydroxyvitamin D2 and D3 Using Liquid Chromatography-Tandem Mass Spectrometry Ada Munar, Clinton Frazee III, and Uttam Garg
£161.99
Humana Mitotic Exit
Book SynopsisA Transgenic Method to Measure Mitotic Exit in Drosophila Embryos.- Generation of Cdc20 RNAi-Sensitive Cell Lines to Study Mitotic Exit.- Overexpression of GFP Fusions of Regulators of the SAC from Arabidopsis thaliana.- Production of SAC Proteins in a Baculovirus-Based Heterologous Gene Expression System.- Quantitative Live-Cell Imaging to Study Chromatin Segregation and Nuclear Reformation.- Determination of Anaphase Duration by Time-Lapse Microscopy in Budding Yeast.- Visualizing Cartwheel Disassembly Process During Mitosis in Fixed and Live Cells by Fluorescence Microscope.- FRET-FLIM for the Study of Protein-Protein Interactions Underpinning Mitosis Checkpoints.- Using FRET to Define Cdk1-Dependent Ordering of Events during Exit from Second Meiotic M-Phase in Oocytes.- Time-Lapse Imaging to Analyze Cell Fate in Response to Antimitotics.- The Role of Polo-Like Kinase 1 (PLK1) O-GlcNAcylation in Mitosis.- Luciferase-Based
£186.99
Arcler Press Biochemistry
Book SynopsisThis text explores the chemical processes that occur in living organisms. It provides a comprehensive overview of the molecular basis of life, including topics cell biology, metabolism, genetics, and molecular biology. The book is designed to help students and researchers understand the fundamental principles of biochemistry and the complex interactions that occur within cells. Whether you are studying biochemistry for the first time or looking to deepen your knowledge, this book is an essential resource for anyone interested in the science of life.Table of Contents Chapter 1 Introduction to Biochemistry Chapter 2 Biomolecules Chapter 3 Energy and Metabolism Chapter 4 Protein Synthesis and Folding Chapter 5 Gene Expression Chapter 6 DNA and RNA Chapter 7 Metabolic Pathways in the Human Body Chapter 8 Sustainable Chemical and Biochemical Technologies
£87.20
Arcler Education Inc Introduction to Biophysical Chemistry
Book SynopsisBiophysical chemistry is a multidisciplinary field that uses models of chemistry and physics to comprehend organic systems by interpreting the quantitative, energetics, structural, functional and interactional context of the physical nature. In this introductory volume, the authors provide insight into the significance of biophysics chemistry as a developing area of science and the wide variety of strategies that have been applied to explain the related phenomena. The book discusses a few specific methods for reviewing biological molecules and how they function. A few of these procedures use environmental and semi-environmental factors as that for organic particles in the natural environment. The book focuses on different methods that up to date are utilized in biophysical chemistry. Every method comes with a brief definition and is cited with proper reference for future research.Table of Contents Chapter 1 Principles of Physical Chemistry (Basic Rules and Constants) Chapter 2 Thermodynamics Chapter 3 Chemical Equilibria Chapter 4 Chemical Kinetics with Enzymology Chapter 5 Membranes and Surfaces in Biological Systems Chapter 6 Protein Conformation Chapter 7 Mass Spectrometry Chapter 8 Nanopore-Based Measurements Chapter 9 Fluorimetry and its Applications Chapter 10 The Future of Biophysical Chemistry
£143.20
RSC Publishing Therapeutic Potential of Gold Complexes
Book SynopsisGlobally, millions of deaths are reported annually due to cancers, bacterial, fungal, viral and parasitic diseases, diabetes and inflammatory conditions. Metal complexes have been extensively developed and tested for anticancer, antibacterial, antifungal, antiviral, antiparasitic, antidiabetic and anti-inflammatory properties. It is quite encouraging to learn that quite a lot of metal complexes with interesting therapeutic properties have been developed.This concise book reviews the recent advances made in the development of gold complexes as therapeutics, highlighting the different ligand architectures that have been used for their development. The mechanisms of therapeutic action, the development of nanodrugs and the toxicity issues of gold complexes are all discussed in detail. Finally, the book reflects on the future research and development in gold complexes as therapeutic agents. Postgraduates and researchers in drug discovery and development interested in metal-based therapeutics and biomedical applications of gold will find this book enlightening.
£141.55
Springer Nature Switzerland AG The Art of Writing Reasonable Organic Reaction
Book SynopsisIntended for students of intermediate organic chemistry, this text shows how to write a reasonable mechanism for an organic chemical transformation. The discussion is organized by types of mechanisms and the conditions under which the reaction is executed, rather than by the overall reaction as is the case in most textbooks. Each chapter discusses common mechanistic pathways and suggests practical tips for drawing them. Worked problems are included in the discussion of each mechanism, and "common error alerts" are scattered throughout the text to warn readers about pitfalls and misconceptions that bedevil students. Each chapter is capped by a large problem set.Table of ContentsChapter 1. The Basics. 1. Structure and Stability of Organic Compounds o Conventions of Drawing Structures; Grossman's Rule o Lewis Structures; Resonance Structures o Molecular Shape; Hybridization o Aromaticity 2. Bronsted Acidity and Basicity o pKa Values o Tautomerism 3. Kinetics and Thermodynamics 4. Getting Started at Drawing a Mechanismo Reading and balancing organic reaction equationso Determining which bonds are made and broken in a reaction 5. Classes of Overall Transformations 6. Classes of Mechanisms o Polar Mechanisms§ Nucleophiles§ Electrophiles and Leaving Groups§ Acidic and Basic Conditions; The pKa Rule§ A Typical Polar Mechanismo Free-Radical Mechanismso Pericyclic Mechanismso Transition-Metal-Catalyzed and -Mediated Mechanisms 7. Summary8. End of Chapter Problems Chapter 2. Polar Reactions under Basic Conditions. 1. Introduction to Substitution and Elimination o Substitution by the SN2 Mechanism o β-Elimination by the E2 and E1cb Mechanisms o Predicting Substitution vs. Elimination 2. Addition of Nucleophiles to Electrophilic π Bonds o Addition to Carbonyl Compounds o Conjugate Addition; The Michael Reaction 3. Substitution at C(sp2)–X s Bonds o Substitution at Carbonyl C o Substitution at Alkenyl and Aryl C o Metal Insertion; Halogen–Metal Exchange 4. Substitution and Elimination at C(sp3)–X σ Bonds o Substitution by the SRN1 Mechanism o Substitution by the Elimination–Addition Mechanism o Substitution by the One-Electron Transfer Mechanism o Metal Insertion; Halogen–Metal Exchange o α-Elimination; Generation and Reactions of Carbenes 5. Base-Promoted Rearrangements o Migrations from C to C o Migrations from C to Oo Migrations from C to N o Migrations from B to C or O 6. Two Multistep Reactions o The Swern Oxidation o The Mitsunobu Reaction 7. Summary8. End of Chapter Problems Chapter 3. Polar Reactions under Acidic Conditions. 1. Carbocations o Carbocation Stability o Carbocation Generation; The Role of Protonation o Typical Reactions of Carbocations; Rearrangements 2. Substitution and β-Elimination Reactions at C(sp3)–X o Substitution by the SN1 and SN2 Mechanisms o Elimination by the E1 Mechanism o Predicting Substitution vs. Elimination 3. Electrophilic Addition to Nucleophilic C=C π Bonds 4. Substitution at Nucleophilic C=C π Bonds o Electrophilic Aromatic Substitution o Aromatic Substitution of Anilines via Diazonium Salts o Electrophilic Aliphatic Substitution 5. Nucleophilic Addition to and Substitution at Electrophilic π Bonds. o Heteroatom Nucleophiles o Carbon Nucleophiles 6. Catalysis Involving Iminium Ions 7. Summary8. End of Chapter Problems Chapter 4. Pericyclic Reactions. 1. Introduction o Classes of Pericyclic Reactions o Polyene MOs 2. Electrocyclic Reactions o Typical Reactions o Stereospecificity o Stereoselectivity 3. Cycloadditions o Typical Reactions § The Diels–Alder Reaction § Other Cycloadditions o Regioselectivity o Stereospecificity o Stereoselectivity 4. Sigmatropic Rearrangements o Typical Reactions o Stereospecificity o Stereoselectivity 5. Ene Reactions. 6. Summary7. End of Chapter Problems Chapter 5. Free Radical Reactions. 1. Free Radicals o Stability o Generation from Closed-Shell Species o Typical Reactions o Chain vs. Nonchain Mechanisms 2. Chain Free-Radical Reactions o Substitution Reactions o Addition and Fragmentation Reactions§ Carbon-Heteroatom Bond-Forming Reactions§ Carbon-Carbon Bond-Forming and -Cleaving Reactions 3. Nonchain Free-Radical Reactions o Photochemical Reactions o Reductions and Oxidations with Metals§ Addition of H2 across π Bonds § Reduction of C-X Bonds. Reductive Coupling§ One-Electron Oxidations o Cycloaromatizations 4. Miscellaneous Radical Reactions o 1,2-Anionic Rearrangements; Lone-Pair Inversion o Triplet Carbenes and Nitrenes 5. Summary6. End of Chapter Problems Chapter 6. Transition-Metal-Mediated and -Catalyzed Reactions. 1. Introduction to the Chemistry of Transition Metals o Conventions of Drawing Structures o Counting Electrons § Typical Ligands; Total Electron Count § Oxidation State and d Electron Count o Typical Reactions o Stoichiometric vs. Catalytic Mechanisms 2. Addition Reactions o Late-Metal-Catalyzed Hydrogenation and Hydrometallation (Pd, Pt, Rh) o Hydroformylation (Co, Rh) o Hydrozirconation (Zr) o Alkene Polymerization (Ti, Zr, Sc, and others) o Cyclopropanation, Epoxidation, and Aziridination of Alkenes (Cu, Rh, Mn, Ti) o Dihydroxylation and Aminohydroxylation of Alkenes (Os) o Nucleophilic Addition to Alkenes and Alkynes (Hg, Pd) o Conjugate Addition Reactions (Cu) o Reductive Coupling Reactions (Ti, Zr) o Pauson–Khand Reaction (Co) o Dötz Reaction (Cr) o Metal-Catalyzed Cycloaddition and Cyclotrimerization (Co, Ni, Rh) 3. Substitution Reactions o Hydrogenolysis (Pd)o Carbonylation of Alkyl Halides (Pd, Rh) o Heck Reaction (Pd) o Metal-Caatalyzed Nucleophilic Substitution Reactions: Kumada, Stille, Suzuki, Negishi, Buchwald–Hartwig, Sonogashira, and Ullmann Reactions (Ni, Pd, Cu) o Allylic Substitution (Pd) o Pd-Catalyzed Nucleophilic Substitution of Alkenes; Wacker Oxidationo C–H Activation (Pd, Ru, Rh) o Tebbe Reaction (Ti) o Propargyl Substitution in Co–Alkyne Complexes 4. Rearrangement Reactions o Alkene Isomerization (Rh) o Olefin and Alkyne Metathesis (Ru, W, Mo, Ti) 5. Elimination Reactions o Oxidation of Alcohols (Cr, Ru) o Decarbonylation of Aldehydes (Rh) 6. Summary7. End of Chapter Problems Chapter 7. Mixed Mechanism Problems.
£999.99
Springer International Publishing AG Pharmaceutical Applications of Supramolecules
Book SynopsisThis book outlines the use of supramolecules as different pharmaceutical drugs. Supramolecular chemistry in pharmaceutical sciences is quite a young and rapidly developing field. Supramolecular assemblies might offer an alternative for existing pharmaceutical formulations, as they facilitate the improvement of physicochemical and pharmacological properties i.e., higher bioavailability, better biocompatibility and drug-targeting, fewer multidrug-resistances. This book offers an overview of the recent advances in supramolecular structures and discusses the future aspects and challenges related to the development of these molecules, providing also a perspective on how to overcome these issues. Divided into 13 chapters contributed by experts in their field, the book provides a deeper understanding of intermolecular forces playing pivotal roles in mediating the interactions between chemical molecules and biological systems by focusing on different applications of supramolecular compounds. In this book, readers will find valuable insights into the preparation of supramolecules and the latest research and development trends of supramolecules as anticancer drugs, including liquid-crystalline supramolecular assemblies, and as antimicrobial, antiviral, anti-inflammatory and cardiovascular drugs. Particular attention is given to the application of supramolecules in the fields of biomedicine, bioimaging, and vaccine development. Given its breadth, this book will appeal to a wide readership from researchers and students interested in these fields to professionals in the pharma industry.Table of ContentsTable of Contents (13 Chapters)1. Supramolecules in pharmaceutical science: A brief overviewNidhi Goel* and Naresh Kumar*2. Basic strategy and methods of preparation for supramoleculesVikrant Jayant, Shakeel Alvi and Rashid Ali*3. Research and development of supramolecules as anticancer drugsManjit and Brahmeshwar Mishra*4. Research and development of liquid-crystalline supramolecular assemblies as anticancer drugsAtsushi Yoshizawa*5. Progressive approach of supramolecules towards the advancement of antimicrobial drugsManju Sharma, Nidhi Aggarwal, Sonika Chibh, Avneet Kour, Samraggi Choudhury and Jiban Jyoti Panda*6. Promising functional supramolecules in antiviral drugsKanika Arora, Aakriti Singh, Aaqib Javaid and Shyam lal Mudavath*7. Role of supramolecules in anti-inflammatory drugsPatil Shivprasad Suresh, Anmol and Upendra Sharma*8. Recent advancements of supramolecules in the evolution of cardiovascular drugsMerve Kiremit and Ayse B. Tekinay*9. Development of supramolecules in the field of nanomedicinesAbhishesh Kumar Mehata and M. S. Muthu*10. Supramolecular self-assembled peptide-based nanostructures and their applications in biomedicineUttam Ghosh and Goutam Ghosh*11. Recent advancement of supramolecules in the field of bioimagingUttam Ghosh , Naresh Kumar and Goutam Ghosh*12. Role of supramolecules in vaccine developmentNaresh Kumar* and Nidhi Goel*13. Supramolecules: Future challenges and perspectivesNidhi Goel* and Naresh Kumar*
£132.99
Springer International Publishing AG Biochemistry and Cell Biology of Ageing: Part IV,
Book SynopsisThis book provides an up-to-date overview of key areas of ageing research and bridges the gap between the subcellular events and the reality of ageing as seen in clinical practice.To this end, the reader learns about the historical development and progression of clinical ageing research. All chapters address the biochemistry or cell biology of various ageing events (to the extent that the data are available) and work their way to the clinical understanding we have of ageing. The focus of this volume is on how dietary restriction, virus infection and chronic inflammation affect the ageing process. Additionally, this book discusses how phosphate metabolism and metabolic dysfunction contribute to ageing events and how various organs and tissues (e.g. tendons, ears, heart muscle, and the endocrine system) age. This book follows on from Parts I, II and III of Biochemistry and Cell Biology of Ageing within the Subcellular Biochemistry book series and aims to bring the subcellular and clinical areas into closer contact by including interesting and significant biomedical ageing topics that were not included in the earlier volumes. Comprehensive and cutting-edge, this book is a valuable resource for experienced researchers and early career scientist alike, who are interested in learning more about the fascinating and challenging question of why and how our cells age.Table of ContentsSee Attachments
£999.99
Springer International Publishing AG Naturally Occurring Organohalogen Compounds
Book SynopsisThe present volume is the third in a trilogy that documents naturally occurring organohalogen compounds, bringing the total number — from fewer than 25 in 1968 — to approximately 8,000 compounds to date. Nearly all of these natural products contain chlorine or bromine, with a few containing iodine and, fewer still, fluorine. Produced by ubiquitous marine (algae, sponges, corals, bryozoa, nudibranchs, fungi, bacteria) and terrestrial organisms (plants, fungi, bacteria, insects, higher animals) and universal abiotic processes (volcanos, forest fires, geothermal events), organohalogens pervade the global ecosystem. Newly identified extraterrestrial sources are also documented. In addition to chemical structures, biological activity, biohalogenation, biodegradation, natural function, and future outlook are presented.Table of ContentsNaturally Occurring Organohalogen Compounds.- A Comprehensive Review.
£134.99
de Gruyter The Neuropathogenesis of Mood Disorders
Book Synopsis
£36.40
De Gruyter Biochemie und Physiologie der Alkaloide
Book Synopsis
£134.09
De Gruyter 2. Internationale Arbeitstagung Biochemie Und
Book Synopsis
£134.09
De Gruyter Biochemistry of Ribosomes and MessengerRNA
Book Synopsis
£141.54
De Gruyter Boudinagen und Mikroboudinagen im metagabbrischen
Book Synopsis
£122.35
De Gruyter Über Proteolyse durch intrazelluläre Proteasen
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£122.35
Birkhauser Verlag AG Molecular Determinants of Head and Neck Cancer
Book SynopsisSquamous cell cancers of the head and neck (SCCHN), also known as head and neck cancers (HNC) encompass malignancies of the oral cavity, larynx, nasopharynx and pharynx, and are diagnosed in over 500,000 patients worldwide each year, accounting for 5% of all malignancies. It is estimated that approximately 50,000 patients develop head and neck cancer annually in the United States, of whom approximately 50% succumb to this cancer. For most cases of SCCHN, treatment is multimodal, often combining surgery or irradiation with chemotherapy; even successfully treated patients frequently experience durable and severe side effects. Improving cure rates and reducing chronic morbidity are urgent clinical needs for head and neck cancer. However, in contrast to cancer types such as breast or prostate that have been much studied and have well-defined biology, until recently, relatively few researchers investigated the molecular basis of HNC, making it difficult to design targeted treatments with better efficacy and less debilitating side effects. This volume will provide an overview of the factors contributing to disease pathogenesis, including the recognition of discrete molecular subtypes with distinct etiology, prognosis, and treatment response. This volume will familiarize the reader with the critical signaling pathways and oncogenic drivers for HNC. It will outline the differences between HPV-positive and HPV-negative disease, and how these differences affect treatment choice and outcome. The book will emphasize developments in the past five years, including the growing understanding of the genomic and epigenomic features of the disease based on analysis of next generation sequencing (NGS) data, and timely topics such as the analysis of HNC stem cell populations, non-coding mRNAs, and inflammatory response. It will address exciting new therapeutic approaches such as the use of immunotherapies to treat HNC patients. Overall, the book will provide the reader with current understanding of the biology and treatment of the disease, and describe timely questions that will guide future research aimed at controlling and curing this disease.Trade Review“The book is intended primarily for clinicians, fellows, interns, and trainees, but medical students who may be interested in head and neck cancer but face the challenge of updating themselves on the latest innovations may also find this book very useful. … this is an informative and authoritative book on the subject that includes experiences from clinicians and scientists working on head and neck cancer at some of the most renowned cancer centers in the world.” (Ravi Salgia, Doody's Book Reviews, March, 2019)Table of ContentsChapter 1: Overview: B. Burtness and E. GolemisChapter 2: HER inhibition in HNC: E. Golemis and B. BurtnessChapter 3: MET: T Beck, Fox Chase Cancer CenterChapter 4: PI3K/mTOR/AMPK: C. Chung, Moffit Cancer CenterChapter 5: Jak/STAT: J.R. Grandis, UCSFChapter 6: TGFb: Neiman and Wang, University of ColoradoChapter 7: Wnt and catenin Signaling: MA Kukurusinska, Boston UniversityChapter 8: P53/p63/p73: B. BurtnessChapter 9: DNA damage: R. Bindra, Yale UniversityChapter 10: APOBEC Proteins: K.S. Anderson, Yale UniversityChapter 11: Hypoxia, including HIF1: Q. Le, Stanford UniversityChapter 12: VEGF/Angiogenesis and Antiangiogenic Therapy: A Argiris, Thomas Jefferson UniversityChapter 13: Sequencing/genomic insights: J.Myers, MD AndersonChapter 14: Gene expression, Methylation, and histones: R.Mehra, A.Andrews, S.Peri, Fox Chase Cancer Center and A.Bhatia, Yale UniversityChapter 15: Inflammation: Carter Van Waes, NIHChapter 16:Immunotherapy: Ferris and Baumann, UPMCChapter 17: FAK/Integrin pathway inhibitors: Nils Cordes, DresdenChapter 18: Targeting cell cycle controls: E.GolemisChapter 19: Head and neck cancer stem cells: L.Bourguignon, UCSFChapter 20: Non-coding RNAs: C.Punyadeera, Queensland UniversityChapter 21: Epidemiology of HPV: Ragin and Lui, Fox Chase Cancer CenterChapter 22: DNA damage in HPV-positive SCCHN: WG Yarbrough, Yale UniversityChapter 23: Treatment paradigms in HPV-positive SCCHN: A Cmelak, Vanderbilt University
£999.99
KS Omniscriptum Publishing Verwendung von Milchpeptidasen zur Herstellung
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£999.99
