Description
Table of ContentsPart I: Measurement of Carbohydrate Modification
1. A low-volume, parallel copper-bicinchoninic acid (BCA) assay for glycoside hydrolases
Gregory Arnal, Mohamed A. Attia, Jathavan Asohan, Zhenhuan Lei, Benedikt Golisch, and Harry Brumer
2. Measuring enzyme kinetics of glycoside hydrolases using the 3,5-dinitrosalicylic acid assay
He Li and Lauren S. McKee
3. Analyzing activities of lytic polysaccharide monooxygenases by liquid chromatography and mass spectrometry
Bjørge Westereng, Magnus Ø. Arntzen, Heidi Østby, Jane Wittrup Agger, Gustav Vaaje-Kolstad, and Vincent G.H. Eijsink
4. Carbohydrate depolymerization by intricate cellulosomal systems
Sarah Moraïs, Johanna Stern, Lior Artzi, Carlos M.G.A. Fontes, Edward A Bayer, and Itzhak Mizrahi
5. Biomechanical weakening of paper and plant cell walls by bacterial expansins
Daniel J. Cosgrove, Nathan K. Hepler, Edward R. Wagner, and Daniel M. Durachko
Part II: Quantitation of Carbohydrate Binding
6. Affinity Electrophoresis for Analysis of Catalytic Module-Carbohydrate Interactions
Darrell W Cockburn, Casper Wilkens, and Birte Svensson
7. Quantifying CBM carbohydrate interactions using microscale thermophoresis
Haiyang Wu, Cédric Y. Montanier, and Claire Dumon
8. Qualitative and quantitative characterization of protein-carbohydrate interactions by NMR spectroscopy
Julie M. Grondin, David N. Langelaan, and Steven P. Smith
9. Isothermal Titration Calorimetry for quantification of protein-carbohydrate interactions
Haley A. Brown and Nicole M. Koropatkin
10. Surface plasmon resonance analysis for quantifying protein-carbohydrate interactions
Marie Sofie Møller, Darrell Cockburn, and Casper Wilkens
11. Bioinspired polymer assemblies of plant cell walls for measuring protein-carbohydrate interactions by FRAP
Berangère Lebas and Gabriel Paës
12. CBMs as probes to explore plant cell wall heterogeneity using immunocytochemistry
Louise Badruna, Vincent Burlat, and Cédric Y. Montanier
13. Flow Cytometry-based Detection of Siglec Ligands
Edward N. Schmidt, Jaesoo Jung, and Matthew S. Macauley
Part III: Purification and visualization of carbohydrates
14. Extraction and Verification of Mouse and Human Mucins from Tissue and Fecal Material
Mackenzie Melvin, Noah Fancy, Darrek Kniffen, and Kirk Bergstrom
15. Analysis of complex carbohydrate composition in plant cell wall using Fourier transformed mid-infrared spectroscopy
Ajay Badhan, Yuxi Wang, and Tim A. McAllister
16. Separation and Visualisation of Glycans by Fluorophore-Assisted Carbohydrate Electrophoresis
Mélissa Robb, Joanne K. Hobbs, and Alisdair B. Boraston
17. A rapid protocol for preparing 8-aminopyrene-1,3,6-trisulfonate-labelled glycans for capillary electrophoresis-based enzyme assays
Jolene M. Garber, Osei B. Fordwour, and Wesley F. Zandberg
18. Visualization of Carbohydrate Uptake using Fluorescent Polysaccharides
Greta Reintjes, Leeann Klassen, and D. Wade Abbott
Part IV: Computational methods for studying protein-carbohydrate interactions
19. Long-read Metagenomics and CAZyme discovery
A. Ferrillo, C.M. Kobel, A.Vera-Ponce de León, S.Leanti La Rosa, B.J. Kunath, P.B. Pope, and L.H. Hagen
20. Identification of genes involved in the degradation of lignocellulose using comparative transcriptomics
Gruninger R.J., Tsang A., and McAllister T.A.
21. Isolation and preparation of extracellular proteins from lignocellulose degrading fungi for comparative proteomic studies using mass spectrometry
Gruninger R.J., Tsang A., and McAllister T.A.
