Description

Book Synopsis

FOXO Transcription Factors: A Brief Overview.- Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity.- Identification of DAF-16/FOXO Binding Partners in Caenorhabditis elegans by Immunoprecipitation and Mass Spectrometry.- Measuring FOXO Activity by Using qPCR-Based Expression Analysis of FOXO Target Genes.-   Reporter Gene Assays to Measure FOXO-Specific Transcriptional Activity.- Functional Motif Discovery in FOXO1 through CRISPR/Cas9 Exon Tiling Scan.- Cardiomyocyte-Specific FoxO1 Knockout Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental Considerations using Cre-loxP.- Methods to Study Structure and Dynamics of FOXO Proteins.- Fluorescence Polarization Method to Assess the FOXO Phosphopeptide Interaction with 14-3-3.- Characterization of FOXO3-14-3-3 Interaction by Isothermal Titration Calorimetry.- Identification of Small-Molecule Modulators of FOXO3 through Virtual Screening.- Me

FOXO Transcription Factors

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    A Hardback by Wolfgang Link

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      Publisher: Humana
      Publication Date: 11/21/2024
      ISBN13: 9781071642160, 978-1071642160
      ISBN10: 1071642162
      Also in:
      Biochemistry

      Description

      Book Synopsis

      FOXO Transcription Factors: A Brief Overview.- Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity.- Identification of DAF-16/FOXO Binding Partners in Caenorhabditis elegans by Immunoprecipitation and Mass Spectrometry.- Measuring FOXO Activity by Using qPCR-Based Expression Analysis of FOXO Target Genes.-   Reporter Gene Assays to Measure FOXO-Specific Transcriptional Activity.- Functional Motif Discovery in FOXO1 through CRISPR/Cas9 Exon Tiling Scan.- Cardiomyocyte-Specific FoxO1 Knockout Mice as Tools to Assess Cardiac Hypertrophy and Key Experimental Considerations using Cre-loxP.- Methods to Study Structure and Dynamics of FOXO Proteins.- Fluorescence Polarization Method to Assess the FOXO Phosphopeptide Interaction with 14-3-3.- Characterization of FOXO3-14-3-3 Interaction by Isothermal Titration Calorimetry.- Identification of Small-Molecule Modulators of FOXO3 through Virtual Screening.- Me

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