Molecular biology Books
Springer Fachmedien Wiesbaden Botanisches Grundpraktikum
Book SynopsisDiese einmalige Kombination aus Lehrbuchtext und Praktikumsanleitung bietet sowohl einen theoretischen Hintergrund als auch eine praktische Anleitung, um die Histologie von Pflanzen kennenzulernen – ideal für Bachelor-Studierende. Ohne diese Grundlagen ist ein Verständnis von komplexen pflanzlichen Entwicklungsprozessen, die durch unterschiedliche Zelltypen und Gewebe mit verschiedenen Aufgaben geprägt sind, nicht möglich.In der 3. Auflage sind einige Objekte hinzugekommen, um das Spektrum des Praktikums zu erweitern. Verschiedene Präparate, die den Niederen Pflanzen zuzurechnen sind, wurden aufgenommen, um durch einfach gebaute Organismen das Verständnis für die Funktionsweise vielzelliger Pflanzen zu entwickeln. Das Kapitel Methoden haben die Autoren um die Bedienung des Lichtmikroskops, die Objektpräparation und Färbemethoden erweitert. Das Botanische Grundpraktikum ist vom Umfang her besonders für Bachelorstudierende geeignet.Table of ContentsDie Pflanzenzelle.- Die Sprossachse.- Das Blatt.- Die Wurzel.- Fortpflanzung und Entwicklung.- Methoden.- Lösungen zu den Aufgaben.- Glossar.- Literatur
£32.99
Springer Fachmedien Wiesbaden PCR - Polymerase-Kettenreaktion
Book SynopsisZiel dieses handlichen, deutschsprachigen Laborhandbuches ist es, die Standard- und Spezialanwendungen der PCR in praxisnaher und verständlicher Form darzustellen. Es wendet sich an Diplomanden, Doktoranden, Wissenschaftler und TAs, die die Möglichkeiten der PCR für ihre molekularbiologischen oder diagnostischen Fragestellungen nutzen möchten.In vorliegender 2. Auflage wurden die etablierten Methoden aktualisiert und wichtige neue Applikationen (z.B. Next Generation Sequencing oder die Emulsions-PCR) hinzugefügt. Da die Automation bei den molekularbiologischen Applikationen fortschreitet, erfordern gerade die letztgenannten Methoden immer weniger Handarbeit, wobei das generelle Verständnis über die einzelnen Schritte vorhanden sein muss. Dieses ‚Know-How‘ erhalten Sie im vorliegenden PCR-Methodenbuch.Table of ContentsEinleitung.- Allgemeine PCR-Parameter.- PCR als Detektionsmethode.- PCR als Klonierungsmethode.- PCR für die Standard-Klonierung.- T/A-Cloning.- Ligase-unabhängige-Klonierung (LIC).- UNG-Klonierung.- Surf-Klonierung.- Megaprime-PCR.- RT-PCR.- RACE-PCR.- Quantitative PCR.- Real-Time-PCR.- Colony-PCR.- PCR zur Mutationsanalyse.- Nested-PCR.- DOP-PCR.- Alu- (IRS) PCR.- PCR-Optimierung.- 1-Sekunden-PCR.- Long Distance-PCR.- Genomtypisierung mit der PCR.- Differential Display PCR Firmenverzeichnis Internet-Adressen rund um die PCR Sachregister.
£52.24
Harwood-Academic Publishers Programmed Cell Death
Book SynopsisAlthough general morphological features have been used to consistently identify the changes in cell ultrastructure occurring during apoptosis, as distinct from necrosis, important advances have been achieved more recently in the investigation of the cellular and molecular aspects of this process. This book brings together the latest international research on the complex subject of programmed cell death, and covers such areas as the biochemical mechanisms, introduction of DNA fragmentation, enetic regulation, and the importance of apoptosis in the immune system, particularly during T-cell development, and in cancer. The comparison of a number of common signal transduction pathways with those involved in cell growth highlights an important relationship between apoptosis and the control of cell proliferation.Table of ContentsBiochemical Mechanisms; DNA Fragmentation; Genetic Regulation; Apoptosis in the Immune System; Apoptosis in Cancer.
£209.00
Steidl Publishers Michel Comte: EL & Us
Book Synopsis
£38.40
Taylor & Francis Ltd Molecular Genetics of Drug Resistance
Book SynopsisDrug resistance is a growing problem in today's society. Successful drugs are constantly being developed but there is always the risk that a small percent of the drug's target will be immune. These survivors can then lead to a new population, resistant to the action of this drug. New drugs are continuously under development to combat this problem, but these can, in turn, lead to new resistant populations. This problem is universal whether the target is to destroy a deadly virus, or an insect which is ravaging crop production. Development of new drugs is difficult and time consuming so it is of crucial importance that we understand the processes behind drug resistance. "Molecular Genetics of Drug Resistance" forms a vital and timely review of the genetic processes behind drug resistance. Starting with an overview of the area, each chapter focuses on a particular target with important sections on drug resistance in malaria and in cancer. Each chapter has been written by an acknowledged expert in the field and the careful work of the editors has ensured a consistent approach and presentation. Table of ContentsNatural selection and drug resistance; drug and pesticide resistance in fungi; viral drug resistance; herbicide resistance; drug resistance in insects; the mechanisms of drug action and resistance in malaria; multidrug resistance in mammalian cells mediated by members of the ATP-binding cassette superfamily; drug resistance in cancer chemotherapy; oxidative stress responses in mammalian cells; modulation and circumvention of drug resistance.
£199.50
Taylor & Francis Ltd Molecular Biology of Alzheimer's Disease: Genes
Book SynopsisHighlighting the latest and the most timely aspects of Alzheimer's disease research, this text will enable scientists in related research fields, as well as physicians working with Alzheimer's disease patients, to obtain a quick and complete overview of the current state of the art in one of the most exciting fields in neuroscience research. Leading scientists have contributed articles focusing on key developments in this field. This includes an overview about the pathology, the genetics of familial Alzheimer's disease, proteolytic generation and aggregation of amyloid -peptide, presenilins, risk factors such as ApoE, and transgenic animal models. Some of the latest developments in Alzheimer's disease research, including the effect of presenilin knock outs on amyloid -peptide generation, are also included.Table of Contents1. The Tau Proteins in Alzheimer's Disease 2. The Genetics of Familial Alzheimer's Disease 3. Mutations in Three Genes Are Associated with Early Onset Alzheimer's Disease 4. The Molecular Biology of Presenilin 1 5. Molecular Biology of Presenilin 2 6. Presenilin Proteins and Their Role in Development and Notch Signaling 7. Transgenic Animal Models in the Development of Therapeutic Strategies for Alzheimer's Disease 8. Amyloid Morphology and Pathology of Alzheimer's Disease 9. The Biological Activities and Function of the Amyloid Precursor Protein of Alzheimer's Disease 10. Transgenic Models of Alzheimer's Disease
£218.50
Taylor & Francis Ltd Plant Chromosomes
Book SynopsisThe past two decades have brought with them remarkable progress in plant chromosomal research. The chromosome structure has been clarified in great detail, enabling identification of gene sequences at the microscopic level, which has aided the analysis of biodiversity. Knowledge of chromosome structure has played a crucial role in the improvement of crop species and has far-reaching implications. The manipulation and engineering of chromosomes involves a panoply of novel methods, combining conventional and modern techniques of biotechnology. A working knowledge of such techniques is essential for today's students and researchers, and the plant system, because of totipotency, requires special treatment. This treatise covers all the latest methods involved in the study of evolution, biodiversity, chromosome manipulation and engineering.Table of ContentsPREFACE, SECTION—I CHROMOSOME RESEARCH IN BIOTECHNOLOGY, BIODIVERSITY AND GENETICS, SECTION—II CHROMOSOME ANALYSIS—SCOPE AND ADVANCES, SECTION—III MOLECULAR PATTERN ANALYSIS AND GENE MAPPING IN CHROMOSOMES, SECTION—IV CHROMOSOME MANIPULATION AND ENGINEERING: GENOME AND GENE TRANSFER, SECTION—V MICRODISSECTION AND CHROMOSOME ENGINEERING, BIBLIOGRAPHY, AUTHOR INDEX
£120.00
Pan Stanford Publishing Pte Ltd Molecular Motors in Bionanotechnology
Book SynopsisBiological molecular motors provide most cells with the dynamic systems required for their day-to-day existence. Examples occur in even the simplest organism (e.g. a bacteria virus), and the range of tasks that they carry out is vast. Over the last few years, there has been a large increase in the study of these motors, and it is becoming apparent that many motors will find uses in either bionanotechnology or synthetic biology.Molecular Motors in Bionanotechnology describes a wide range of molecular motors, ranging from chemical motors to biological motors, in a manner that updates, or reviews, both classification of the type of motor and the grouping into families. Many techniques have evolved to study and characterise molecular motors at the single-molecule level (e.g. use of molecular tweezer devices for single-molecule studies). The text introduces the reader to the concepts and benefits of these techniques. In addition, it looks at the structural information and how this helps understand function and, finally, how some of these motors are being used or may be used in the future as part of a synthetic biology approach to building devices and sensors.
£109.25
Pan Stanford Publishing Pte Ltd Bioluminescent Microbial Biosensors: Design,
Book SynopsisThis book describes the design and the use of bioluminescent biosensors. It introduces beginners and experienced researchers starting in the microbiological biosensor domain to the practical aspects of building a luminescent microbial biosensor. It is also a source of information about other applications that use microbial cells. Each chapter focuses as far as possible on the technological conception of the presented biosensor with a clear demonstration of the issues in the design and how to reach the proof of concept. The book is divided into three practical sections facilitating the reader to easily access the information.Table of ContentsLuminescent Biosensors with Free Suspended Cells. Fully Automated Fluidic Analyzer for Food Quality Assessment Using Bioluminescent Biosensors. Technological Design of Optical Bacterial Biosensors for the Online Environmental Monitoring of Water Pollutants. Luminescent Biosensors with Immobilized Cells. Fiber Optic Biosensors for Environmental Monitoring. Non-Fiber Optic Bioluminescent Biosensors. Biosensor with Immobilized Cells and Lensless Imaging Detection Luca CeveniniaA biosensor with Genetically Modified Bacteria Immobilized on a Fiber and a Glass Slide. Development and Characterization of a Living-Cell Bioluminescent Bioreporter Integrated Circuit (BBIC). BOD Sensor with Immobilized Luminous Cells on ChipBioluminescent Whole-Cell Sensors: Integration of Bacterial Reporter Cells onto Hardware Platforms. Luminescent Biosensors with Bioluminescent Sporulated Cells ("Sporosensor"). Luminescent Biosensing Systems Based on Genetically Engineered Spore-Forming Bacteria.
£109.25
The University of Chicago Press Shaping Science with Rhetoric
Book SynopsisIn considering how scientists persuade colleagues to cross the disciplinary divide, this text examines three scientific monographs in their historical contexts: Dodzhansky's "Genetics and the Origin of Species" (1937); Schrodinger's "What is Life?" (1944); and Wilson's "Consilience" (1998).
£30.00
The University of Chicago Press The Evolutionary Biology of Plants
Book SynopsisProvides a comprehensive synthesis of modern evolutionary biology as it relates to plants. This text recounts the saga of plant life from its origins to the radiation of the flowering plants. Through computer-generated walks it shows how living plants might have evolved.
£28.00
The University of Chicago Press Membranes to Molecular Machines
Book SynopsisToday's science tells us that our bodies are filled with molecular machinery that orchestrates all sorts of life processes. When we think, microscopic channelsopen and close in our brain cell membranes; when we run, tiny motorsspin in our muscle cell membranes; and when we see, light operates molecular switchesin our eyes and nerves. A molecular-mechanical vision of life has become commonplace in both the halls of philosophy and the offices of drug companies, where researchers are developing proton pump inhibitors or medicines similar to Prozac. Membranes to Molecular Machines explores just how late twentieth-century science came to think of our cells and bodies this way. This story is told through the lens of membrane research, an unwritten history at the crossroads of molecular biology, biochemistry, physiology, and the neurosciences, that directly feeds into today's synthetic biology as well as nano- and biotechnology. Mathias Grote shows how these sciences not only have made us think differently about life, they have, by reworking what membranes and proteins represent in laboratories, allowed us to manipulate life as active matterin new ways. Covering the science of biological membranes in the United States and Europe from the mid-1960s to the 1990s, this book connects that history to contemporary work with optogenetics, a method for stimulating individual neurons using light, and will enlighten and provoke anyone interested in the intersection of chemical research and the life sciencesfrom practitioner to historian to philosopher. The research described in the book and its central actor, Dieter Oesterhelt, were honored with the 2021 Albert Lasker Basic Medical Research Awardfor his contribution to the development of optogenetics.
£37.05
The University of Chicago Press Morphological Integration
Book SynopsisPioneers in reexamining morphology, Everett Olson and Robert Miller were among the first to explore the concept of the integrated organism in both living and extinct populations. This text contains their findings.
£30.40
The University of Chicago Press Making PCR A Story of Biotechnology
Book SynopsisA behind-the-scenes account of the invention of the polymerase chain reaction, which transformed the practice and potential of molecular biology. This book explores the culture of biotechnology as it emerged at Cetus Corporation in the 1980s, looking at its scientific, social and economic elements.
£23.00
University of Notre Dame Press Evolutionary and Molecular Biology
Book SynopsisThese research papers explore the creative interaction between evolutionary and molecular biology, philosophy and theology. They aim to support the engagement of constructive theology with the natural sciences and explore philosophical and theological elements in ongoing scientific research.Trade Review“The essays in this volume represent the real cutting edge in discussions between Christianity and biology. They provide a careful, sober assessment of biological story in all its complexity. One can only hope that churchpeople will enter into the debate with the care and reasonableness these authors have shown.” —The Christian Century
£51.85
Wiley Molecular Nutrition and Genomics
Book SynopsisThis fascinating book draws its subject matter from a range of relevant disciplines that extend from molecular nutrition, nutritional sciences, and nutrition dietetics through to genetics, genomics, and anthropology.Table of ContentsPREFACE. ACKNOWLEDGMENTS. INTRODUCTION. Chapter 1—Defining Important Concepts. 1.1 Key Concepts in Molecular Biology for the Study of Human Nutrition. 1.2 The Inheritance of Genetic Packets of Information. 1.3 A Brief Overview of Evolutionary Biology and the Ascent of Man. 1.4 The –omics Revolution. Chapter 2—Molecular Mechanisms of Genetic Variation Linked to Diet. 2.1 A Brief History of the Human Diet. 2.2 The Role of Milk in Human Evolution. 2.3 Micronutrients and the Evolution of Skin Pigmentation. 2.4 Micronutrients Optimize Gametogenesis and Reproductive Fecundity. 2.5 Direct Dietary Selection of a Human Metabolomic Profile. 2.6 The Evolution of Taste as a Survival Mechanism. 2.7 The Mystery of Alcohol Dehydrogenase Polymorphisms and Ethanol Toxicity. 2.8 Evolution of Xenobiotic Metabolism in Humans. Chapter 3—Essential Nutrients and Genomic Integrity: Developmental and Degenerative Correlates. 3.1 Micronutrients and Genomic Stability and Function. Chapter 4—Nutrients and Cerebral Function in Human Evolution. 4.1 Human Encephalisation May be Linked to an Evolutionary Reduction in Gut Mass. 4.2 Weaning and Brain Development. 4.3 Micronutrients and the Cerebral Basis of Spirituality and Social Structure. 4.4 Pharmacotoxicology of Plants and Cultural Evolution. Chapter 5—The Evolution of Micronutrient Metabolism. 5.1 Antioxidants, Evolution, and Human Health. Chapter 6—Evolved Refinement of the Human Lifecycle Based on Nutritional Criteria. 6.1 Human Breast Milk—An Evolved Food. 6.2 Conflict between Parent and Offspring over Nutrient Requirements. 6.3 Natural Selection for Foraging Efficiency. 6.4 Evolution of Senescence. Chapter 7—The Evolution of Human Disease. 7.1 The Conflict between Agriculture and Ancestral Genes. 7.2 Obesity: A Chronic Plague of our Affluent Societies. 7.3 Prion Protein Locus and Human Evolution: The Link Between Variant Creutzfeld-Jakob Disease and Cannibalism. Chapter 8—Contemporary Dietary Patterns that Work: The Mediterranean Diet. 8.1 Tomatoes. 8.2 Olive Oil. 8.3 Red Wine. 8.4 Bioflavonoids. 8.5 Fish. Chapter 9—Some Non-Micronutrient Essential and Nonessential Nutrients with Molecular and Possible Evolutionary Impact. 9.1 Lecithins. 9.2 Lipid-Derived First Messengers—The Eicosanoids.1 9.3 Isoflavones—Genomic and Nongenomic Influence at the Estrogen Receptor. 9.4 Phytic Acid. Chapter 10—Natural Food Toxins and the Human Diet. 10.1 Dietary Zootoxins. 10.2 Dietary Phytotoxins. Chapter 11—Nutrigenomics. 11.1 What is Nutrigenomics? 11.2 Genetic Buffering Underpins Nutrigenomic Relationships. Chapter 12—The Evolution of Protein Function. Chapter 13—Leading Edge Laboratory Tools in Nutrigenomics and Human Evolutionary Studies. 13.1 Denaturing HPLC. 13.2 DNA Sequencing. 13.3 Nucleic Acid Microchip Techniques. 13.4 The Polymerase Chain Reaction. 13.5 Protein Mass Spectrometry. 13.6 Bioinformatics. References. Index.
£98.96
John Wiley & Sons Inc Computational Intelligence
Book SynopsisThis book highlights the importance and recent success of computational intelligence methods over a diverse range of bioinformatics problems. It encourages others to use these methods and approaches in their research, while also serving as an introduction to computational intelligence methods and applications to the consumers of the research.Table of ContentsPreface. Contributors. Part One Gene Expression Analysis and Systems Biology. 1. Hybrid of Neural Classifi er and Swarm Intelligence in Multiclass Cancer Diagnosis with Gene Expression Signatures (Rui Xu, Georgios C. Anagnostopoulos, and Donald C. Wunsch II). 1.1 Introduction. 1.2 Methods and Systems. 1.3 Experimental Results. 1.4 Conclusions. 2. Classifying Gene Expression Profi les with Evolutionary Computation (Jin-Hyuk Hong and Sung-Bae Cho). 2.1 DNA Microarray Data Classifi cation. 2.2 Evolutionary Approach to the Problem. 2.3 Gene Selection with Speciated Genetic Algorithm. 2.4 Cancer Classifi ction Based on Ensemble Genetic Programming. 2.5 Conclusion. 3. Finding Clusters in Gene Expression Data Using EvoCluster (Patrick C. H. Ma, Keith C. C. Chan, and Xin Yao). 3.1 Introduction. 3.2 Related Work. 3.3 Evolutionary Clustering Algorithm. 3.4 Experimental Results. 3.5 Conclusions. 4. Gene Networks and Evolutionary Computation (Jennifer Hallinan). 4.1 Introduction. 4.2 Evolutionary Optimization. 4.3 Computational Network Modeling. 4.4 Extending Reach of Gene Networks. 4.5 Network Topology Analysis. 4.6 Summary. Part Two Sequence Analysis and Feature Detection. 5. Fuzzy-Granular Methods for Identifying Marker Genes from Microarray Expression Data (Yuanchen He, Yuchun Tang, Yan-Qing Zhang, and Rajshekhar Sunderraman). 5.1 Introduction. 5.2 Traditional Algorithms for Gene Selection. 5.3 New Fuzzy-Granular-Based Algorithm for Gene Selection. 5.4 Simulation. 5.5 Conclusions. 6. Evolutionary Feature Selection for Bioinformatics (Laetitia Jourdan, Clarisse Dhaenens, and El-Ghazali Talbi). 6.1 Introduction. 6.2 Evolutionary Algorithms for Feature Selection. 6.3 Feature Selection for Clustering in Bioinformatics. 6.4 Feature Selection for Classifi cation in Bioinformatics. 6.5 Frameworks and Data Sets. 6.6 Conclusion. 7. Fuzzy Approaches for the Analysis CpG Island Methylation Patterns (Ozy Sjahputera, Mihail Popescu, James M. Keller, and Charles W. Caldwell). 7.1 Introduction. 7.2 Methods. 7.3 Biological Signifi cance. 7.4 Conclusions. Part Three Molecular Structure and Phylogenetics. 8. Protein–Ligand Docking with Evolutionary Algorithms(René Thomsen). 8.1 Introduction. 8.2 Biochemical Background. 8.3 The Docking Problem. 8.4 Protein–Ligand Docking Algorithms. 8.5 Evolutionary Algorithms. 8.6 Effect of Variation Operators. 8.7 Differential Evolution. 8.8 Evaluating Docking Methods. 8.9 Comparison between Docking Methods. 8.10 Summary. 8.11 Future Research Topics. 9. RNA Secondary Structure Prediction Employing Evolutionary Algorithms (Kay C. Wiese, Alain A. Deschênes, and Andrew G. Hendriks). 9.1 Introduction. 9.2 Thermodynamic Models. 9.3 Methods. 9.4 Results. 9.5 Conclusion. 10. Machine Learning Approach for Prediction of Human Mitochondrial Proteins (Zhong Huang, Xuheng Xu, and Xiaohua Hu). 10.1 Introduction. 10.2 Methods and Systems. 10.3 Results and Discussion. 10.4 Conclusions. 11. Phylogenetic Inference Using Evolutionary Algorithms(Clare Bates Congdon). 11.1 Introduction. 11.2 Background in Phylogenetics. 11.3 Challenges and Opportunities for Evolutionary Computation. 11.4 One Contribution of Evolutionary Computation: Graphyl. 11.5 Some Other Contributions of Evolutionary computation. 11.6 Open Questions and Opportunities. Part Four Medicine. 12. Evolutionary Algorithms for Cancer Chemotherapy Optimization (John McCall, Andrei Petrovski, and Siddhartha Shakya). 12.1 Introduction. 12.2 Nature of Cancer. 12.3 Nature of Chemotherapy. 12.4 Models of Tumor Growth and Response. 12.5 Constraints on Chemotherapy. 12.6 Optimal Control Formulations of Cancer Chemotherapy. 12.7 Evolutionary Algorithms for Cancer Chemotherapy Optimization. 12.8 Encoding and Evaluation. 12.9 Applications of EAs to Chemotherapy Optimization Problems. 12.10 Related Work. 12.11 Oncology Workbench. 12.12 Conclusion. 13. Fuzzy Ontology-Based Text Mining System for Knowledge Acquisition, Ontology Enhancement, and Query Answering from Biomedical Texts (Lipika Dey and Muhammad Abulaish). 13.1 Introduction. 13.2 Brief Introduction to Ontologies. 13.3 Information Retrieval form Biological Text Documents: Related Work. 13.4 Ontology-Based IE and Knowledge Enhancement System. 13.5 Document Processor. 13.6 Biological Relation Extractor. 13.7 Relation-Based Query Answering. 13.8 Evaluation of the Biological Relation Extraction Process. 13.9 Biological Relation Characterizer. 13.10 Determining Strengths of Generic Biological Relations. 13.11 Enhancing GENIA to Fuzzy Relational Ontology. 13.12 Conclusions and Future Work. References. Appendix Feasible Biological Relations. Index.
£90.86
John Wiley & Sons Inc Molecular Pathology in Drug Discovery and
Book SynopsisThe pharmaceutical industry is looking for new ways to better identify populations that will react positively to new drugs, and molecular pathology can fill that need.Trade Review“Molecular Pathology in Drug Discovery and Development is a well-written book that provides both an overview of how biomarkers may be developed and detailed explanations of how to measure and analyze said biomarkers.” (The Yale Journal of Biology and Medicine, March 2010)Table of ContentsPreface. Contributors. 1 MOLECULAR PATHOLOGY AND DRUG DEVELOPMENT (Franz Fogt and J. Suso Platero). 1.1. General Pathology. 1.2. General Aspects. 1.3. Molecular Pathology, the Molecular Way. 1.4. Application of Molecular Pathology. 1.5. Molecular Pathology in Drug Development. 1.6. Pharmaceutical Drug Development. References. 2 MOLECULAR PATHOLOGY IN ONCOLOGY TARGET AND DRUG DISCOVERY (Rolf-P. Ryseck, Ricardo Attar, Matthew V. Lorenzi, and Brent A. Rupnow). 2.1. Introduction. 2.2. History of Chemotherapy and Cancer Drug Discovery. 2.3. Target-Based Drug Discovery. 2.4. Utilization of Molecular Pathology in the Discovery of Novel Cancer Targets. 2.5. Hit Identifi cation and In Vitro Lead Optimization. 2.6. Implications for Molecular Pathology in Cancer Drug Development and Use. 2.7. Summary and Future Considerations. References. 3 MOLECULAR PATHOLOGY AND TRANSCRIPTIONAL PROFILING IN EARLY DRUG DEVELOPMENT (Cornelia Liedtke, Lajos Pusztai, and W. Fraser Symmans). 3.1. Introduction. 3.2. Biomarkers in Clinical Setting and in Early Drug Development. 3.3. Advantages of Biomarker Implementation. 3.4. Changing Paradigm in Clinical Drug and Biomarker Development. 3.5. Promises of Transcriptional Profiling. 3.6. Biomarker Development and Validation Using Microarray Analysis. 3.7. Neoadjuvant Chemotherapy as an Intriguing Model for Biomarker Development. 3.8. Transcriptional Profi ling for Identifi cation of Individual Genes as Biomarkers. 3.9. Transcriptional Profi ling for the Definition of Multigene Predictors Using .Transcriptional Profiling. 3.10. Novel Tools for Pathway Analysis. 3.11. Implementation of Biomarkers into the Clinical Setting. 3.12. Conclusion. References. 4 MOLECULAR PATHOLOGY IN NONCLINICAL SAFETY ASSESSMENT (Richard A. Westhouse). 4.1. Introduction. 4.2. Drug Development. 4.3. Drug Discovery. 4.4. Biopharmaceuticals. 4.5. Summary. References. 5 TOXICOGENOMICS IN DRUG DEVELOPMENT (Wayne R. Buck and Eric A. G. Blomme). 5.1. Introduction. 5.2. Brief Overview of Large-Scale Gene Expression Technologies. 5.3. Analysis of Microarray Data. 5.4. Application of Toxicogenomics in Drug Development. 5.5. Considerations for Toxicogenomic Study Design. 5.6. Overview of Major Regulatory Developments Related to Use of Toxicogenomics in Drug Discovery and Development. 5.7. Summary. References. 6 MOLECULAR PATHOLOGY AS A WAY TO FIND THE RIGHT DOSE FOR A DRUG (F. Rojo, A. Rovira, S. Serrano, and J. Albanell). 6.1. Introduction. 6.2. Anti-EGFR-Targeted Therapies: The Pharmacodynamic Experience. 6.3. Molecular Pathology with Small Molecules Gefitinib and Erlotinib. 6.4. Molecular Pathology with Cetuximab and Other Monoclonal Antibodies to EGFR. 6.5. Proteasome Inhibitors: Pharmacodynamics on Blood Samples. 6.6. Pharmacodynamics with Rapamycin Analogs. 6.7. Second Generation of Targeted Therapies: Multitarget Agents. 6.8. Conclusions and Perspectives: Phase 0 Clinical Trials. References. 7 MOLECULAR PATHOLOGY IN LIFE-CYCLE MANAGEMENT IN DRUG DEVELOPMENT (Martha Quezado, Carlos A. Torres-Cabal, and David Berman). 7.1. Introduction. 7.2. Molecular Pathology Techniques. 7.3. Practical Applications of Molecular Pathology Biomarkers. 7.4. Conclusion. References. 8 MOLECULAR PATHOLOGY AND MOLECULAR THERAPY (Hewei Li). 8.1. Introduction. 8.2. Molecular Therapy Strategies. 8.3. Molecular Therapy Clinical Trials. References. 9 MOLECULAR PATHOLOGY: IMMUNOHISTOCHEMISTRY ASSAYS IN DRUG DEVELOPMENT PERFORMED BY A CONTRACT RESEARCH LABORATORY (Frank Lynch and Steve Bernstein). 9.1. Immunohistochemistry Is the Technique of Microscopic Visualization of Target Proteins in Tissue Sections Using Specific Antibodies. 9.2. Basics of the IHC Assay. 9.3. Immunohistochemistry Assay Development. 9.4. Sending a Study to a Contract Laboratory vs. Running In-house. 9.5. Choosing and Working with an Outside Laboratory— Keys for a Succe.ssful Relationship—What to Do before a Slide Is Stained. 9.6. Running and Managing Outsourced Clinical Studies 9.7. Applications of IHC in Drug Discovery and Development Process. 9.8. Conclusion. References. 10 QUANTIFICATION OF MOLECULAR PATHOLOGY: COLORIMETRIC IMMUNOHISTOCHEMISTRY (Raphael Marcelpoil). 10.1. Introduction. 10.2. Imaging Devices and Systems. 10.3. Quantifi cation: Introduction to Colorimetric Image Analysis. 10.4. Measuring Colorimetric Information. 10.5. Chromogen Separation. 10.6. Measuring Information. 10.7. Conclusion. References. 11 AQUA® TECHNOLOGY AND MOLECULAR PATHOLOGY (Mark Gustavson, Marisa Dolled-Filhart, Jason Christiansen, Robert Pinard, and David Rimm). 11.1. Introduction. 11.2. AQUA Technology—How It Works. 11.3. Standardization. 11.4. Quantification. 11.5. Localization. 11.6. Multiparametric Analysis. 11.7. Application of AQUA Technology to Drug Discovery and Companion Diagnostics. 11.8. Summary and Conclusions. References. Index.
£116.96
John Wiley & Sons Inc Searching for Molecular Solutions
Book SynopsisThis book compares empirical discovery methods for biologically relevant molecules with serendipitous discovery and rational design, and highlights the general approach to empirical discovery and its advantanges/disadvantages.Trade Review"This is a valuable resource that compares empirical discovery and rational design, while also considering the strengths and limitations of empirical pathways to molecular discovery. Scientists, professionals, and students interested in general aspects of molecular discovery will find this book extremely useful." (Doody's, 19 August 2011) Table of ContentsAcknowledgments xi Introduction xiii 1 If It Works, It Works: Pragmatic Molecular Discovery 1 2 Empirical Miracles: Nature's Precedents for Empirical Solutions 22 3 Evolution's Gift: Natural Somatic Diversification and Molecular Design 61 4 Evolution While You Wait 103 5 The Blind Protein Maker 145 6 The Blind Shaper of Nucleic Acids 187 7 Evolving and Creating Recognition Molecules 234 8 Molecules Small and Large 275 9 An Empirical--Rational Loop Forwards 315 10 Exploring the Limits 343 Glossary 365 Bibliography 374 References in Figure Legends 440 Index 443
£123.45
John Wiley & Sons Inc Plant Phenolics and Human Health
Book SynopsisA collection of current knowledge of phytochemicals and health Interest in phenolic phytochemicals has increased as scientific studies indicate these compounds exhibit potential health benefits. With contributions from world leaders in this research area, Plant Phenolics and Human Health: Biochemistry, Nutrition, and Pharmacology offers an essential survey of the current knowledge on the capacity of specific micronutrients present in ordinary diets to fight disease. The coverage in this resource: Explains the presence and biochemical properties of phenolics present in fruits and vegetables, as well as in foods derived from their plant sources Provides biochemical explanations on how certain plant phenolics fight cardiovascular and neurodegenerative diseases, cancer, and other widespread pathologies Focuses on certain phenolics, e.g., flavonoids, stilbenes, and curcuminoids, and provides insights on the biocheTrade Review"Some 62 eminent researchers from 14 countries have contributed to the book's 21 chapters, each offering a comprehensive account of what is currently happening at the cutting edge of such intersecting disciplines as food science, nutrition, medicine, and pharmaceutical research. Learned updates to this topic of plant phenolics will probably be forthcoming every five to ten years." (CHOICE, 2010) Table of ContentsPREFACE. CONTRIBUTORS. 1 Dietary Flavonoids and Phenolic Compounds (Indu B. Jaganath and Alan Crozier). 2 Bioavailability of Flavanols and Phenolic Acids (Laure Poquet, Michael N Clifford, and Gary Williamson). 3 Biochemical Actions of Plant Phenolics Compounds: Thermodynamic and Kinetic Aspects (Cesar G. Fraga, Gulcin Sagdicoglu Celep, and Monica Galleano). 4 Flavonoids–Membrane Interactions: Consequences for Biological Actions (Sandra V. Verstraeten, Cesar G. Fraga, and Patricia I. Oteiza). 5 The Biochemistry Behind the Potential Cardiovascular Protection by Dietary Flavonoids (Wai Mun Loke, Jonathan M. Hodgson, and Kevin D. Croft). 6 Dietary Flavanols: Biochemical Basis of Short-Term and Longer-Term Vascular Responses (Tankred Schewe, Yvonne Steffen, Elisabeth Kravets and Helmut Sies). 7 Green Tea Catechins: Anticancer Effects and Molecular Targets (Naghma Khan and Hasan Mukhtar). 8 Flavonols: Metabolism, Bioavailability, and Health Impacts (Junji Terao). 9 Flavonols: Biochemistry Behind Cardiovascular Effects (Francisco Pérez-Vizcaı´no and Juan Duarte). 10 Metabolism, Bioavailability, and Analysis of Dietary Isoflavones (Adrian A. Franke, Brunhild M. Halm, Kerry Kakazu and Xingnan Li). 11 Phytoestrogens Up-regulate Antioxidant Genes (Consuelo Borrás and Jose Viña). 12 Dietary Isoflavones: Cardiovascular Actions and Activation of Cellular Signalling Pathways (Richard C. M. Siow and Giovanni E. Mann). 13 Bioavailability and Metabolism of Resveratrol (Cristina Andres-Lacueva, Mireia Urpi-Sarda, Raul Zamora-Ros, and Rosa M. Lamuela-Raventos). 14 Resveratrol: Biochemistry and Functions (Samarjit Das, Hannah R. Vasanthi, and Dipak K. Das). 15 Resveratrol: The Biochemistry Behind its Anticancer Effects (Joydeb K. Kundu and Young-Joon Surh). 16 Curcumin: The Biochemistry Behind Its Anticancer Effects (Preetha Anand, Ajaikumar B. Kunnumakkara, and Bharat B. Aggarwal). 17 Plant Phenolic Compounds: Modulation of Cytoprotective Enzymes and Nrf2/ARE Signaling (Siwang Yu, Ka Lung Cheung, Wenge Li and Ah-Ng Kong). 18 Phenolics in Aging and Neurodegenerative Disorders (Vittorio Calabrese, Marzia Perluigi, Carolin Cornelius, Raffaella Coccia, Fabio Di Domenico, Giovanni Pennisi, Chiara Cini and Albena T. Dinkova-Kostova). 19 Natural Phenolics and Metal Metabolism in Neurodegenerative Diseases (Baolu Zhao). 20 Epidemiology behind Fruit and Vegetable Consumption and Cancer Risk with Focus on Flavonoids (Marta Rossi, Eva Negri, Cristina Bosetti, Claudio Pelucchi, and Carlo La Vecchia). 21 Phenylpropanoid Metabolism in Plants: Biochemistry, Functional Biology, and Metabolic Engineering (Alberto B. Landolino and Douglas R. Cook). INDEX.
£135.85
John Wiley and Sons Ltd Handbook of Molecular Microbial Ecology I
Book SynopsisThe premiere two-volume reference on revelations from studying complex microbial communities in many distinct habitats Metagenomics is an emerging field that has changed the way microbiologists study microorganisms. It involves the genomic analysis of microorganisms by extraction and cloning of DNA from a group of microorganisms, or the direct use of the purified DNA or RNA for sequencing, which allows scientists to bypass the usual protocol of isolating and culturing individual microbial species. This method is now used in laboratories across the globe to study microorganism diversity and for isolating novel medical and industrial compounds. Handbook of Molecular Microbial Ecology is the first comprehensive two-volume reference to cover unculturable microorganisms in a large variety of habitats, which could not previously have been analyzed without metagenomic methodology. It features review articles as well as a large number of case studies, based largely on oTrade Review“Handbook of Molecular Microbial Ecology I is an invaluable reference for researchers in metagenomics, microbiology, and environmental microbiology; those working on the Human Microbiome Project; microbial geneticists; molecular microbial ecologists; and professionals in molecular microbiology and bioinformatics.” (Bois et Forets des Tropiques, 2011) "Handbook of Molecular Microbial Ecology I is an invaluable reference for researchers in metagenomics, microbiology, and environmental microbiology; those working on the Human Microbiome Project; microbial geneticists; molecular microbial ecologists; and professionals in molecular microbiology and bioinformatics." (TMCnet.com, 8 November 2011) Table of ContentsPreface. Contributors. 1. Introduction (Frans J. de Bruijn). Background Chapters. 2. DNA reassociation yields broad-scale information on metagenome complexity and microbial diversity (V. Torsvik). 3. Diversity of 23S rRNA genes within individual prokaryotic genomes (Zhiheng Pei). 4. Use of the rRNA operon and genomic repetitive sequences for the identification of bacteria (A. Nascimento). 5. Use of different PCR primer-based strategies for characterization of natural microbial communities (James Prosser). 6. Horizontal gene transfer and recombination shape mesorhizobial populations in the gene center of the host plants Astragalus luteolus and Astragalus ernestii in Sichuan, China (Xiaoping Zhang). 7. Amplified rDNA restriction analysis (ARDRA)for identification and phylogenetic placement of 16S-rDNA clones (Menachim Sklarz). 8. Clustering-based peak alignment algorithm for objective and quantitative analysis of DNA fingerprinting data (Satoshi Ishii). The Species Concept. 9. Population genomics informs our understanding of the bacterial species concept (Margaret Riley). 10. Genome analysis of Streptococcus agalactiae: Implication for the microbial “pan-genome” (R. Rappuoli). 11. Metagenomic insights into bacterial species (Kostas Konstantinidis). 12. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology (E. Stackebrandt). 13. Metagenomic Approaches for the Identification of Microbial Species (David Ward). Metagenomics. 14. Microbial Ecology in the age of metagenomics (Jianping Xu). 15. The enduring legacy of small rRNA in microbiology (Susan Tringe). 16. Pitfalls of PCR-based rRNA gene sequence analysis: an update on some parameters (Stackebrandt). 17. Empirical testing of 16S rRNA gene PCR primer pairs reveals variance in target specificity and efficacy not suggested by in silico analysis (Sergio Morales and Bill Holben). 18. The impact of next-generation sequencing technologies on (meta)genomics (George Weinstock). 19. Accuracy and quality of massively parallel DNA pyrosequencing (Susan Huse and David Mark Welch). 20. Environmental shotgun sequencing: Its potential and challenges for studying the hidden world of microbes (Jonathan Eisen). 21. Comparison of random sequence reads versus 16S rDNA sequences for estimating the biodiversity of a metagenomic library (C. Manischan). 22. Metagenomic libraries for functional screeing (Svein Valla). 23. GC Fractionation Allows Comparative Total Microbial Community Analysis, Enhances Diversity Assessment, and Facilitates of Minority Populations of Bacteria (Bill Holben). 24. Enriching plant microbiota for a metagenomic library construction (Ying Zeng). 25. Towards Automated Phylogenomic Inference (Wu and Eisen). 26. Integron first gene cassettes: a target to find adaptive genes in metagenomes (Christine Cagnon). 27. High-resolution metagenomics: assessing specific functional types in complex microbial communities (Christoserdova). 28. Gene-targeted –metagenomics (GT-metagenomics) to explore the extensive diversity of genes of interest in microbial communities (J. Tiedje). 29. Phylogenetic screening of metagenomic libraries using homing endonuclease restriction and marker insertion (Torsten Thomas). 30. ArrayOme- & tRNAcc-facilitated mobilome discovery: comparative genomics approaches for identifying rich veins of novel bacterial DNA sequences (Hong-Yu OU). 31. Sequence-Based Characterization of Microbiomes by Serial Analysis of Ribosomal Sequence Tags (SARST) (Zhongtang Yu). Consortia and Databases. 32. The metagenomics of plant pathogen-suppressive soils (J.D. Van Elsas). 33. Soil Metagenomic Exploration of the Rare Biosphere (Pascal Simonet and Timothy Vogel). 34. The BIOSPAS consortium: Soil Biology and agricultural production (Luis Wall). 35. The Human Microbiome Project (George Weinstock). 36. The Ribosomal Database Project: sequences and Software for high-throughput rRNA analysis (J. R. Cole, G. M. Garrity and Jim Tiedje). 37. The metagenomics RAST server- a public resource for the automatic phylogenetic and functional analysis of metagenomes (Folker Meyer). 38. The EBI Metagenomics Archive, Integration and Analysis resource (Apweiler). Computer Assisted Analysis. 39. Comparative metagenome analysis using MEGAN (Suparna Mitra and Daniel Huson). 40. Phylogenetic binning of metagenome sequence samples (Alice C. McHardy). 41. Gene prediction in metagenomic fragments with Orphelia: A large scale machine learning approach (Katharina Hoff). 42. Binning metagenomic sequences using seeded GSOm (Sen-Lin Tang). 43. Iterative read mapping and assembly allows the use of a more distant reference in metagenomic assembly (Bas E. Dutilh). 44. Ribosomal RNA identification in metagenomic and metatranscriptomic datasets (Li). 45. SILVA: comprehensive databases for quality checked and aligned ribosomal RNA sequence data compatible with ARB (Frank Gloeckner). 46. ARB; a software environment for sequence data (Wolfgang Ludwig). 47. The Phyloware Project: A software framework for phylogenomic virtue (Daniel Frank). 48. Metasim- A sequencing simulator for genomics and metagenomics (Daniel Richter). 49. ClustScan: an integrated program package for the detection and semi-automatic annotation of secondary metabolite clusters in genomic and metagenomic DNA datasets (Daslav Hranueli). 50. MetaGene; Prediction of prokaryotic and phage genes in metagenomic sequences (Noguchi). 51. primers4clades, a web server to design lineage-specific PCR primers for gene-targeted metagenomics (Pablo Vinuesa). 52. A parsimony approach to biological pathway reconstruction/inference for genomes and metagenomes (Y. Ye). 53. ESPRIT: estimating species richness using large collections of 16S rRNA data (Yijun Sun). Complementary Approaches. 54. (Meta) genomics approaches in systems biology (Manuel Ferrer). 55. Towards “focused metagenomics”: a case study combining DNA stable-isotope probing, multiple displacement amplification and metagenomics (J. Colin Murrell). 56. Galbraith, E. A., D. A. Antonopoulos, K. E. Nelson, and B. A. White . Suppressive subtractive hybridization reveals extensive horizontal transfer in the rumen metagenome (Bryan White). Microarrays. 57. GeoChip: A high throughout metagenomics technology for dissecting microbial community functional structure (J. Zhou). 58. Phylogenetic microarrays (PhyloChips) for analysis of complex microbial communities (Eoin Brodie). 59. Phenomics and Phenotype MicroArrays: Applications Complementing Metagenomics (Barry Bochner). 60. Microbial persistence in low biomass, extreme environments: The great unknown (Kasthuri Venkateswaran). 61. Application of phylogenetic oligonucleotide microarrays in microbial analysis (Nian Wang). Metatranscriptomics. 62. Isolation of mRNA from environmental microbial communities for metatranscriptomic analyses (P. Schenk). 63. Comparative day/night metatrancriptomic analysis of microbial communities in the North Pacific subtropical gyre (Rachel Poretski). 64. The “double RNA” approach to simultaneously assess the structure and function of environmental microbial communities by meta-transcriptomics (Tim Urich and Christa Schleper). 65. Soil eukaryotic diversity, a metatranscriptomic approach (Marmeisse). Metaproteomics. 66. Proteomics for the analysis of environmental stress responses in prokaryotes (Mark Suter). 67. Microbial community proteomics (Paul Wilmes). 68. Synchronicity between population structure and proteome profiles: A metaproteomic analysis of Chesapeake Bay bacterial communities (Feng Chen). 69. High-Throughput Cyanobacterial Proteomics: Systems-level Proteome Identification and Quantitation (Phillip Wright). 70. Protein Expression Profile of an Environmentally Important Bacterial Strain: the Chromate Response of Arthrobacter sp. strain FB24 (K. Henne). Metabolomics. 71. The small molecule dimension: Mass spectrometry based metabolomics, enzyme assays, and imaging (Trent R. Northen). 72. Metabolomics: high resolution tools offer to follow bacterial growth on a molecular level (Lucio Marianna and Philipp Schmitt-Kopplin). 73. Metabolic profiling of plant tissues by electrospray mass spectrometry (Heather Walker). 74. Metabolite identification, pathways and omic integration using online databases and tools (Matthew Davey). Single cell analysis. 75. Application of cytomics to separate natural microbial communities by their physiological properties (Susann Müller). 76. Capturing microbial populations for environmental genomics (A. Pernthaler/Wendeberg). 77. Microscopic single-cell isolation and multiple displacement amplification of genomes from uncultured prokaryotes (Peter Westermann).
£257.36
John Wiley and Sons Ltd Molecular Ecology 2e
Book SynopsisThis second edition provides an accessible introduction to the many diverse aspects of this subject. The book unites theory with examples from a wide range of taxa in a logical and progressive manner, and its straightforward writing style makes subjects such as population genetics and phylogenetics highly comprehensible to its readers.Table of ContentsPreface. Acknowledgements. 1 Molecular Genetics in Ecology. What is molecular ecology? The emergence of molecular ecology. Protein allozymes. Allozymes as genetic markers. An unlimited source of data. Mutation and recombination. Is genetic variation adaptive? Polymerase chain reaction. Getting data from PCR. Quantitative PCR. Overview. Chapter summary. Useful websites and software. Further reading. Review questions. 2 Molecular Markers in Ecology. Understanding molecular markers. Modes of Inheritance. Nuclear versus organelle. Haploid chromosomes. Identifying hybrids. Uniparental markers: A cautionary note Molecular markers. Co-dominant markers. Dominant markers. Overview. Chapter summary. Useful websites and software. Further reading. Online activities. Review questions. 3 Genetic Analysis of Single Populations. Why Study Single Populations? What is a population? Quantifying genetic diversity. Hardy-Weinberg equilibrium. Estimates of genetic diversity. Haploid diversity. Choice of marker. What Influences Genetic Diversity? Genetic drift. What is effective population size? Quantifying census population size. Quantifying effective population size. Demographic influences on Ne. Ne, genetic drift and genetic diversity. Population bottlenecks. Founder effects and invasive species. Natural selection. The major histocompatibility complex. Reproduction. Overview. Chapter summary. Useful websites and software. Further reading. Online activities. Review questions. 4 Genetic Analysis of Multiple Populations. Why study multiple populations? Quantifying Population Subdivision. Genetic distance. F-statistics. Interpreting FST. Non-a priori identification of populations. Quantifying Gene Flow. Direct methods. Indirect methods. Assignment tests. What Influences Gene Flow? Barriers to dispersal. Landscape genetics. Metapopulations. Interspecific interactions. Hybridization. Population differentiation: genetic drift and natural selection. Gene flow and genetic drift. Gene flow and local adaptation. Overview. Chapter summary. Useful websites and software. Further reading. Online activities. Review questions. 5 Studying Ecologically Important Traits: Ecogenomics, QTL Analysis, and Reverse Genetics. Studying ecologically important traits. cDNA libraries and ESTs. Microarrays. How do microarrays work? Probes. Verifying differences in gene expression. Microarray applications. Microarrays and community ecology of microorganisms. Microorganism functions. Microarrays and genotyping. Connecting genotype to phenotype. Reverse genetics. QTL analysis. Linkage mapping. QTL mapping. QTL mapping of ecologically important traits. Overview. Chapter Summary. Useful Websites and Software. Further Reading. Review Questions. 6 Phylogeography. What is Phylogeography? Molecular markers in phylogeography. Organelle versus nuclear markers. Repetitive versus non-repetitive markers. Molecular clocks. Bifurcating trees. The coalescent. Applying the coalescent. Networks. Nested Clade Phylogeographic Amalysis and statistical phylogeography. The distributions of genetic lineages. Subdivided populations. Dispersal and vicariance. Comparative phylogeography. Regional concordance. Continental concordance. European post-glacial recolonization routes. Dispersal and invasive species. Allele sharing between species. Lineage sorting. Hybridization. Overview. Chapter summary. Useful websites and software. Further reading. Online activities. Review questions. 7 Behavioural Ecology. Why use molecules to study behaviour? Mating systems. Parentage analysis. Extra-pair fertilizations. Social breeding. Social insects. Manipulating sex ratios. Sex ratio conflicts. Sex-biased dispersal. Nuclear and mitochondrial markers. Relatedness. FST values. Assignment tests. Spatial autocorrelation. Concordant results. Predators and prey. Identifying prey. Predation and conservation. Overview. Chapter summary. Useful websites and software. Further reading. Online activities. Review questions. 8 Conservation Genetics. The need for conservation. Taxonomy. Species concepts. DNA barcoding. Subspecies. Conservation units. Hybrids. Population size, genetic diversity and inbreeding. Inbreeding depression. Heterozygosity fitness correlations. Self-fertilization. Inbreeding avoidance Outbreeding depression. Translocations. Genetic rescue. Source populations. Restoration genetics. Captive breeding. Maximizing genetic diversity. Captive inbreeding and outbreeding. Genetic diversity banks. Overview. Chapter summary. Useful websites and software. Further Reading. Online activities. Review questions. Glossary. Answers to Review Questions. References. Index.
£123.26
John Wiley and Sons Ltd Protein Kinase CK2
Book SynopsisProtein kinases play a critical role in cellular processes that impact overall organismal health and function. Of the kinases that collectively make up the Human Kinome, CK2 has garnered special attention because of its significant role in the generation of the human phosphoproteome. The role CK2 plays in the development of cancer and other disease has also made it of significant interest for its potential role in future therapeutics. Protein Kinase CK2 comprehensively brings together the varied work being done on this critical enzyme. Protein Kinase CK2 is logically divided into three sections. The first section reviews key molecular and structural aspects of the enzyme. The second section looks at functional aspects of CK2 and the diverse roles it plays in cellular development, function, and health. The final section focuses on CK2 and cancer, looking at the impacts of the kinase on neoplastic development and its rapidly developing role as a therapeutic agent.Table of ContentsContributors xi Preface xv Part 1 Molecular and Structural Aspects 1 1 Structural Bases of Protein Kinase CK2 Function and Inhibition 3 Karsten Niefind and Roberto Battistutta Introduction 3 Basic Structure/Function Relationships of CK2 8 CK2 Inhibitors 37 Conclusions and Outlook 60 Acknowledgments 61 2 The Interactome of Protein Kinase CK2 76 Mathias Montenarh and Claudia Götz Introduction 76 From the Outside to the Inside: Interaction of CK2 with Membrane Proteins 78 Regulating Gene Expression: Interaction of CK2 with Components of Signaling Cascades, Transcription Factors and DNA Modifying Enzymes 82 Mastering Nucleic Acid Functions: Interaction of CK2 with Proteins of the Replication, Transcription, and Translation Machinery of the Cell 87 Let It Roll: Interaction of CK2 with Cell Cycle Regulatory Proteins 90 Guardian Angels: Interaction of CK2 with Proteins That Maintain the Cellular Integrity 93 Live and Let Die: Interaction of CK2 with Proteins of the Apoptotic Pathway 96 Highways in the Cell: Interaction of CK2 with the Cytoskeleton and Motor Proteins 96 Collaborating with the Enemy: Interaction of CK2 with Proteins Implicated in Viral Infections 98 Last But Not Least: Miscellaneous 99 Concluding Remarks 101 3 CK2 Contribution to the Generation of the Human Phosphoproteome 117 Luca Cesaro and Mauro Salvi Kinases Contribution to the Human Phosphoproteome 117 CK2 Substrate Specificity 118 Subphosphoproteomes of Proteins with Specific Functions 122 Subphosphoproteomes of Cellular Compartments 123 Absolute Quantification of Yeast Phosphoproteome Reflects the Constitutive Activity of CK2 125 Conclusions 125 Acknowledgments 126 Part 2 Functional Aspects 129 4 CK2 in Embryonic Development 131 Laura Macias Alvarez, Jesus Revuelta-Cervantes, and Isabel Dominguez CK2 in Yeast Biology 131 CK2 in Invertebrate Development 135 CK2 in Vertebrate Development 139 CK2 in Plant Development 148 CK2 in Animal Developmental Signaling Pathways 149 Discussion 155 Outlook 157 Acknowledgments 158 5 Protein Kinase CK2: At the Crossroads of Pathways Controlling Cell Proliferation and Survival 169 Michelle Gabriel and David W. Litchfield General Introduction 169 Protein Kinase CK2 170 CK2 in Cancer 171 Involvement of CK2 in Signaling Pathways Controlling Proliferation and Death 172 Concluding Remarks 182 Acknowledgments 183 6 The Role of Protein Kinase CK2 in the p53 Response 190 David W. Meek Protein Kinase CK2 190 The p53 Network 191 The Interaction Between p53 and CK2 192 Regulation of p53 by Phosphorylation of Ser392 193 Proposed Mechanism for Regulation of p53 Phosphorylation at Ser392 (the “CK2” Site) 194 Phosphorylation of p53 by CK2 in a Physiological Context? 196 A Broader Role for CK2 in Regulating the p53 Network? 197 7 The Pivotal Role of CK2 in the Kinome-Targeting Hsp90 Chaperone Machinery 205 Yoshihiko Miyata Protein Kinase CK2 205 Hsp90: A Major Molecular Chaperone 206 Co-Chaperones That Regulate Hsp90 Function 208 Hsp90 and Signaling Protein Kinases 212 Phosphorylation and the Regulation of Hsp90 by CK2 217 Phosphorylation of Cdc37 by CK2 218 A Crucial Role of CK2-Dependent Phosphorylation in the Functional Regulation of Cdc37 220 Regulation of the Cdc37 Phosphorylation Cycle 220 Regulatory Phosphorylation of FKBP52 by CK2 223 Phosphorylation of p23 by CK2 224 Targeting the CK2-Cdc37-Hsp90 Trinity for Cancer Chemotherapy 224 Conclusion 227 8 CK2: A Global Regulator of Cell Survival 239 Barbara Guerra and Olaf-Georg Issinger CK2 and Cell Survival: Strategies, Methods, and Techniques for Exploring Its Role 239 CK2 and Cellular Death 244 Role of CK2 in DNA Damage 246 Role of the Individual CK2 Subunits in Cell Survival 248 CK2 Status in Non-neoplastic Cells 250 CK2 Activity and Expression in Neoplasia 252 CK2 in Heterotransplanted Tumors in Nude Mice 252 CK2 Holoenzyme and Its Subunits 253 Tumor Hypoxia 255 Conclusion 257 9 Specific Features of Plant CK2 267 Marta Riera, Isabel Cristina Vélez-Bermúdez, Tommaso Legnaioli, and Montserrat Pagès Introduction 267 CK2α Catalytic Subunits 268 CK2β Regulatory Subunits 274 CK2 Holoenzyme 276 Physiological Role of CK2 in Plants 279 Part 3 Ck2 And Neoplasia 291 10 The Oncogenic Potential of CK2 293 David C. Seldin and Esther Landesman-Bollag Introduction 293 CK2 Overexpression in Human Cancer 294 CK2 Is Overexpressed in Animal Models of Cancer 297 CK2 Overexpression in Transgenic Mice Leads to Cancer 297 Possible Targets of CK2 in Cancer: Wnt, NF-κB, and PI3-Kinase Pathways 298 Conclusions 301 Acknowledgments 301 11 Addiction of Cancer Cells to CK2: Survival at All Costs or Achilles’ Heel? 305 Maria Ruzzene Many Substrates, One Major Role 305 A Lateral Player 308 “More Necessary” for Some Cells 310 To Survive at All Costs 310 A Novel Achilles’ Heel of Cancer Cells 311 The Right Weapons 312 Acknowledgments 313 12 CK2 Suppression of Apoptosis and Its Implication in Cancer Biology and Therapy 319 Janeen H. Trembley, JingJiang Wu, Gretchen M. Unger, Betsy T. Kren, and Khalil Ahmed Introduction 319 CK2 Dynamics in Cell Growth and Cell Death 320 CK2 and Hallmarks of Cancer 323 CK2 as Target of Cancer Therapy 329 Acknowledgments 333 13 Protein Kinase CK2 in Normal and Malignant Hematopoiesis 344 Francesco Piazza Hematopoiesis and Blood Tumors: General Concepts 344 CK2 Role in Mouse Embryonic Development: Insights into CK2 Involvement in Blood Development 345 CK2-Directed Regulation of Hematopoiesis-Associated Molecules and Signal Transduction Pathways 346 Role of CK2 in Hematologic Malignancies 351 CK2 in Blood Tumors Arising from Lymphocytes 352 CK2 in Blood Tumors Arising from Myeloid Cells 353 Conclusions 355 14 Role of CK2 in the Control of Cell Plasticity in Breast Carcinoma Progression 363 Odile Filhol, Alexandre Deshiere, and Claude Cochet Introduction 363 Dysregulation of CK2 in Mammary Tumorigenesis 364 CK2 as a Guardian of Epithelial Cell Integrity 364 Unbalanced Expression of CK2 Subunits Is Correlated with Hypoxia and EMT-Related Markers 366 CK2β Subunit Silencing Induces EMT-Like Morphological Changes 367 Gene Expression Profiling 373 CK2β Silencing Triggers Snail1 Induction 373 Overexpression of SIX1 in CK2β-Depleted Cells 374 Conclusions 375 Acknowledgments 377 15 CK2 as a Logical Target in Cancer Therapy: Potential for Combining CK2 Inhibitors with Various Classes of Cancer Therapeutic Agents 383 Denis Drygin Introduction 384 Suppression of Apoptosis 385 PI3K-Akt-mTOR Signaling 390 Promotion of Angiogenesis 393 Hsp90 Machinery 395 NF-κB Transcription 399 Wnt Signaling 402 Epithelial-Mesynchemal Transition 405 DNA Damage Repair 405 Other Pathways 409 Concluding Remarks 411 Acknowledgments 411 Appendix: Ck2 And Its False Sisters: The Recent Solution Of A Very “Cold Case” 440 Index 442
£163.76
John Wiley & Sons Inc LigandReceptor Energetics
Book SynopsisA foundation for quantitative perspectives and a framework for interpreting experimental observations. Researchers in the life sciences who are unaware of the origins of the fundamental concepts and theoretical constructs in ligand-receptor energetics may fail to recognize the hidden assumptions and premises in their interpretations of observed phenomena. This book offers a detailed exposition of these fundamentals and of the treatment of multiple equilibria in successive steps of the binding of ligands to receptors. It also describes the calculations and meanings of energetic quantities for ligand-receptor complexes. Ligand-Receptor Energetics is the only book on this topic that is both accessible to beginners and extremely useful for experienced investigators. It features numerous specific examples; tables of literature results; extensive, up-to-date thermodynamic data; graphical representations of ligand bonding concepts; and four helpful appendices. Topics coveredTable of ContentsNumber of Liganded Molecules. Affinities: From a Site Perspective. Affinities: From a Stoichiometric Perspective. Affinities: From a Ghost-Site Perspective. Facts and Fantasies from Graphical Analyses. Numerical Evaluations of Stoichiometric Binding Constants. Affinity Profiles. Thermodynamic Perspectives. Forces of Interaction. Molecular Scenarios. Appendices. Index.
£99.86
John Wiley & Sons Inc Advances in Enzymology and Related Areas of
Book SynopsisEnzymes are the primary catalysts of life processes. This book covers the latest advances in enzymology, explaining the behaviour of enzymes and how they can be utilized to develop novel drugs, synthesize known and novel compounds, and understand evolutionary processes.Table of ContentsContributors. Preface. Abstracts. Molecular Paleoscience: Systems Biology from the Past (Steven A. Benner, Slim O. Sassi, and Eric A. Gaucher). A Structure-Centric View of Protein Evolution, Design, and Adaptation (Eric J. Deeds and Eugene I. Shakhnovich). Mechanisms of Protein Evolution and Their Application to Protein Engineering (Margaret E. Glasner, John A. Gerlt, and Patricia C. Babbitt). Novel Enzymes Through Design and Evolution (Kenneth J. Woycechowsky, Katherina Vamvaca, and Donald Hilvert). Author Index. Subject Index.
£152.95
John Wiley & Sons Inc Guide to Mutation Detection
Book SynopsisAs genomic science and potential gene therapies continue to develop, the detection of mutations plays an increasingly central role in diverse areas of biology. With innumerable detection techniques available spanning multiple disciplines, the choice of the best method for a particular study can prove exceedingly difficult.Trade Review"…a useful tool…" (American Journal of Human Genetics, August 2005) “…an honest attempt to assemble useful and practical information within this book.” (ChemBioChem, 2005; Vol.6; 6)Table of ContentsPreface. PART I: REVIEWS. 1. Genotype to Phenotype (Sue Malcolm). 2. Frequency and Nature of Mutations and the Methods to Detect Them (Mireille Claustres). 3. A Full-Coverage, High-Resolution Human Chromosome 22 Microarray for Clinical and Research Applications (Jan P. Dumanski and Patrick G. Buckley). 4. Detection of Large Heterozygous Deletions and Duplications (Mario Tosi). 5. Principles of Mutation Scanning by DHPLC (John F. Harvey). 6. Enzymatic and Chemical Cleavage Methods (Richard G. H. Cotton). 7. Quality in Mutation Detection (David E. Barton). 8. Comparison of Scanning Methods for BRCA1 Mutation Analysis (Norbert Arnold). 9. MeltMADGE: Economical Very High-Throughput Mutation Scanning Using Thermal Ramp Electrophoresis in Conjunction with MADGE (Microplate Array) Gels (Ian N. M. Day, Mohammed A. Aldahmesh, Lema Haddad, Khalid K. Alharbi, Xiao-he Chen, Hamid Rassoulian, Steve E. Humphries, Emmanuel Spanakis, and Diana Eccles). 10. High-Throughput SNP Genotyping by Minisequencing Primer Extension Using Oligonucleotide Microarrays (Katarina Lindroos, Ulrika Liljedahl, Snaevar Sigurdsson, and Ann-Christine Syvänen). 11. Mutation Databases (Rania Horaitis and Richard G. H. Cotton). 12. Mass Spectrometry for High-Throughput Genotyping (Ivo Glynne Gut). 13. Locus-Specific Mutation Databases and Nomenclature Annex (Christophe Beroud). PART II: METHODS. 14. Extraction of Nucleic Acids (Graham R. Taylor). 15. Polymerase Chain Reaction and ARMS (Mark D. Robinson and Graham R. Taylor). 16. Minisequencing and Capillary Electrophoresis (Darren Cuthbert-Heavens). 17. SNP Genotyping Using Tag-Array Minisequencing (Snaevar Sigurdsson and Ann-Christine Syvänen). 18. Dynamic Allele-Specific Hybridization (Darren Cuthbert-Heavens). 19. Fluorescence Polarization SNP Detection (PerkinElmer Life Sciences, Inc.). 20. Pyrosequencing (Pyrosequencing AB). 21. Single Nucleotide Polymorphisms (SNPs) by Electronic Hybridization (Nanogen). 22. Manual SSCP and Heteroduplex Analysis Gels (Mark D. Robinson). 23. Fluorescent SSCP: Slab Gels and Capillary Electrophoresis (Lucy Jenkins and Ruth Charlton). 24. Denaturing Gradient Gel Electrophoresis (Gert de Voss). 25. Protein Truncation Test (Mark D. Robinson). 26. DHPLC (Claire F. Taylor). 27. Temperature-Gradient Capillary Electrophoresis (Spectrumedix Corp.). 28. Chemical Cleavage of Mismatch and Enzyme Mismatch Cleavage (Richard G. H. Cotton). 29. Automated Dye Terminator Sequencing (Ruth Charlton). 30. Mutation Detection with the Staden Package (James Bonfield and David Judge). 31. Gene Dosage by Semiquantitative PCR (Kim Flintoff). 32. Gene Dosage by Multiplex Amplifiable Probe Hybridization (David Cockburn and Helen Snowden). 33. Gene Dosage by Multiplex Ligatable Probe Amplification (Jan Schouten and Ruth Charlton). 34. Array CGH (Jan P. Dumanski and Patrick G. Buckley). 35. Microplate Array Diagonal Gel Electrophoresis (MADGE) (Ian N. M. Day). Index.
£134.06
John Wiley & Sons Inc Bioinstrumentation
Book SynopsisWhile many books on medical instrumentation only cover hospital instrumentation, this comprehensive book also encompasses measurements in the growing fields of molecular biology and biotechnology, including applications such as cell engineering, tissue engineering, and biomaterials.Table of ContentsPreface. 1. Measurement Systems (Kevin Hugo). 2. Basic Concepts of Electronics (Hong Cao). 3. Analysis of Molecules in Clinical Medicine (Mat Klein). 4. Surface Characterization in Biomaterials and Tissue Engineering (Jorge E. Monzon). 5. Hematology (Susanne Clark Cazzanti). 6. Cellular Measurements in Biomaterials and Tissue Engineering (Jeffrey S. Schowalter). 7. Nervous Systems (Jang-Zern Tsai). 8. Heart and Circulation (Supan Tungjitkusolmun). 9. Lung, Kidney, Bone, and Skin (Shilpa Sawale). 10. Body Temperature, Heat, Fat, and Movement (Chao-Min Wu). Index.
£222.26
John Wiley & Sons Inc The Harvey Lectures Series 94 19981999
Book SynopsisThe Harvey Society was founded in 1905 by thirteen New York scientists and physicians with the purpose of forging a closer relationship between the purely practical side of medicine and the results of laboratory investigation. The Society distributes scientific knowledge in selected areas of anatomy, physiology, pathology, bacteriology, pharmacology, and physiological and pathological chemistry through public lectures, which are published annually. Series 94, 1998-1999 covers themes in neurogenetic studies, the role of tyrosine phosphorylation in cell growth and disease, the biology of the epidermis and its appendages, and the phenotypic diversity of monogenic disease.Table of ContentsThe Phenotypic Diversity of Monogenic Disease: Lessons from the Thalassemias (D. Weatherall). Humble Starts and Conserved Themes in Neurogenetic Studies (Y. Jan & L. Jan). Beauty is Skin Deep: The Fascinating Biology of the Epidermis and Its Appendages (E. Fuchs). The Final Step in Gene Expression: Caperonin Assisted Protein Folding (P. Sigler). The Role of Tyrosine Phosphorylation in Cell Growth and Disease (T. Hunter). Visualizing Antigen Recognition (M. Davis). Lessons from the Immune System: From Catalsis to Materials (P. Schultz). Former Officers of the Harvey Society. Cumulative Author Index. Active Members.
£208.95
John Wiley & Sons Inc Chronic Obstructive Pulmonary Disease
Book SynopsisChronic obstructive pulmonary disease (COPD), characterized by progressive chronic airflow obstruction, is the most common respiratory disorder of adults in the developed world and is the fourth main cause of death in the US. COPD has received little attention compared with other respiratory diseases such as asthma and lung cancer.Trade Review"The panel of speakers included some of the most distinguished scientists and physicians working in the field of COPD." (Biochemie, No. 83, 2001)Table of ContentsOverview of the pathology/pathogenesis Overview of current therapies Genetics Mucus hypersecretion in COPD Mechanisms of exacerbation Epithelial and mesenchymal cells Macrophage control of inflammation: negative pathways of regulation of inflammatory cytokines Neutrophils Lymphocytes Oxidants/antioxidants Proteases and anti-proteases - I Proteases and anti-proteases - II Alveolus formation: critical period, plasticity and retinoid regulation Systemic effects of COPD Potential novel therapies for COPD
£137.66
John Wiley & Sons Inc Advances in Enzymology and Related Areas of
Book SynopsisThe articles contained in this volume should aid research workers, teachers and writers of textbooks on biochemistry. They aim to provide reviews of the progress made in the various branches of enzymology.Table of ContentsChemical and Genetic Probes of the Active Site ofD-Ribulose-1,5-Bifphosphate Carboxylase/Oxygenase: A RetrospectiveBased on the Three-Dimensional Structure (F. Hartman & M.Harpel). Phenylalanine Hydroxylating System (S. Kaufman). Post-Translational Modification of Proteins (R. Krishna & F.Wold). The Role of Metal Clusters and MgATP in Nitrogenase Catalysis (L.Mortenson, et al.). Myristoyl CoA: Protein N-Myristoyl-Transferase (D. Rudnick, etal.). Development of Enzyme-Based Methods for DNA Sequence Analysis andTheir Applications in the Genome Projects (R. Wu). Indexes.
£396.10
John Wiley & Sons Inc Dictionary of Biochemistry and Molecular Biology
Book SynopsisIn response to the expansion of knowledge in biochemistry and molecular biology, the Second Edition of this reference has been completely revised and updated, with approximately 16,000 new entries. Names of specific compounds and other substances have been substantially enlarged, and definitions have been expanded for clarity and precision. Information is drawn from over 500 books and 1,000 articles, including recommendations of the Commission on Biochemical Nomenclature, the International Union of Pure and Applied Chemistry, and the International Union of Biochemistry. Terms used by biochemists from a broad range of sciences, such as chemistry, immunology, genetics, virology, biophysics, and microbiology, are included. Abbreviations, both standard and nonstandard, are also provided, as well as cross-referenced synonymous expressions.
£413.96
Wiley The LockAndKey Principle
a huge range and FREE tracked UK delivery on ALL orders.
£474.26
John Wiley & Sons Inc Quantitative Molecular Pharmacology and
Book SynopsisQuantitative Molecular Pharmacology and Informatics in Drug Discovery Michael Lutz, Section Head, Cheminformatics Group and Terry Kenakin, Principal Research Scientist, Glaxo Wellcome Research and Development, Research Triangle Park, NC, USA Quantitative Molecular Pharmacology and Informatics in Drug Discovery combines pharmacology, genetics and statistics to provide a complete guide to the modern drug discovery process. The book discusses the pharmacology of drug testing and provides a detailed description of the statistical methods used to analyze the resulting data. Application of genetic and genomic tools for identification of biological targets is reviewed in the context of drug discovery projects. Covering both the theoretical principles upon which the techniques are based and the practicalities of drug discovery, this informative guide. * outlines in step-by-step detail the advantages and disadvantages of each technology and approach and links these to the type of chemicalTrade Review"...a timely and important book..." "I recommend this book to pharmaceutical company statisticians..." --Biometrics, September 2000 "...covers a tremendous amount of ground....I recommend this book to pharmaceutical statisticians..." --Technometrics, 2000Table of ContentsDrug Discovery. Measurement of Drug Affinity. Efficacy. Pharmacological Assays Used in Screening for Therapeutic Ligands. Finding the Optimal Assay Format for the Chemical Target. Mathematical and Statistical Framework for Problems in Drug Discovery. Statistical Methods for Target Identification and Validation. Experimental Design. Analysis and Interpretation of Data. Index.
£232.16
Harvard University Press The Hidden Mechanics of Exercise
Book SynopsisThe Hidden Mechanics of Exercise reveals the microworld of the body in motion, from motor proteins that produce force to enzymes that extract energy from food, and tackles questions athletes ask: What should we ingest before and during a race? How does a hard workout trigger changes in our muscles? Why does exercise make us feel good?Trade ReviewGillen’s enjoyable account emphasizes the molecules and protein structures that allow us to move, run, jump, control fuel use, and regulate adaptations to exercise training… Refreshingly, Gillen approaches the subject from the system down rather than the molecule up. Throughout the book, he emphasizes how tiny changes in protein structures scale up to produce whole-body movements… Gillen offers exercise enthusiasts wishing to understand the science behind their training an interesting read. The book also serves as an engaging primer for exercise-science students who want to begin to understand some of the underlying molecular mechanisms. The Hidden Mechanics of Exercise introduces concepts that make the step to the specialized textbook or research article easier. At the same time, researchers studying the behavior of the individual molecules may find Gillen’s account enlightening in regard to the functional implications of their work at the whole-body level. -- Stephen D. R. Harridge * Science *Anyone who has an interest in how the body and mind works in a sporting context will not only gain an insight and much knowledge, but also enjoy the way that the message is put across. * Athletics Weekly *To most of us, what happens deep inside our bodies when we exercise is a mysterious black box. This entertaining and illuminating book lucidly explains for nonspecialists the marvels of how molecules literally move a body. Gillen provides the ideal introduction to the physiology of exercise for anyone interested in how bodies work. -- Daniel E. Lieberman, author of The Story of the Human Body
£32.36
Princeton University Press Biomolecular Feedback Systems
Book SynopsisProvides an introduction to the principles and tools for modeling, analyzing, and synthesizing biomolecular systems. This book begins with modeling tools such as reaction-rate equations, reduced-order models, stochastic models, and specific models of important core processes.Trade Review"The authors did superbly in combining the biophysical processes and corresponding mathematics... This book serves both as a primer and a reference for constructing synthetic biological circuits with special focus on biomolecular feedback. It nicely bridges the gap between fields with a concise biological introduction, and approachable mathematics."--Harold Bien and Gabor Balazsi, Quarterly Review of Biology "This book promises much for the reader with a background in both biochemistry and mathematics. Such a reader will not only learn how to analyse models for bioengineered bimolecular systems but they will have the insights to both build these systems and to 'tune' the biochemistry to obtain desired parameter values."--Mark Nelson, Gazette of the Australian Mathematical SocietyTable of ContentsPreface vii 1 Introductory Concepts 1 1.1 Systems biology: Modeling, analysis and role of feedback 1 1.2 The cell as a system 8 1.3 Control and dynamical systems tools 11 1.4 Input/output modeling 18 1.5 From systems to synthetic biology 22 1.6 Further reading 28 2 Dynamic Modeling of Core Processes 29 2.1 Modeling chemical reactions 29 2.2 Transcription and translation 44 2.3 Transcriptional regulation 55 2.4 Post-transcriptional regulation 70 2.5 Cellular subsystems 81 Exercises 86 3 Analysis of Dynamic Behavior 89 3.1 Analysis near equilibria 89 3.2 Robustness 103 3.3 Oscillatory behavior 113 3.4 Bifurcations 124 3.5 Model reduction techniques 127 Exercises 133 4 Stochastic Modeling and Analysis 139 4.1 Stochastic modeling of biochemical systems 139 4.2 Simulation of stochastic systems 154 4.3 Input/output linear stochastic systems 157 Exercises 164 5 Biological Circuit Components 169 5.1 Introduction to biological circuit design 169 5.2 Negative autoregulation 171 5.3 The toggle switch 177 5.4 The repressilator 180 5.5 Activator-repressor clock 184 5.6 An incoherent feedforward loop (IFFL) 189 5.7 Bacterial chemotaxis 191 Exercises 203 6 Interconnecting Components 205 6.1 Input/output modeling and the modularity assumption 205 6.2 Introduction to retroactivity 206 6.3 Retroactivity in gene circuits 209 6.4 Retroactivity in signaling systems 214 6.5 Insulation devices: Retroactivity attenuation 219 6.6 A case study on the use of insulation devices 236 Exercises 239 7 Design Tradeoffs 243 7.1 Competition for shared cellular resources 243 7.2 Stochastic effects: Design tradeoffs in systems with large gains 253 Exercises 257 Bibliography 259 Index 267
£74.80
Princeton University Press Strange Glow The Story of Radiation
Book SynopsisTrade ReviewWinner of the 2017 PROSE Award in History of Science, Medicine & Technology, Association of American Publishers One of Physics World's Top Ten Books of the Year, 2016 One of Choice's Outstanding Academic Titles for 2016 #6 on The Telegraph's Top 50 Books of the Year 2016 One of Smithsonian Magazine's Best Science Books of 2016 Shortlisted for Physics World's Book of the Year 2016 "Jorgensen walks readers through the history of humanity's interaction with radiation... [Strange Glow] is a solid, accessible work, but perhaps its most beneficial aspect is that Jorgensen equips readers with enough knowledge to make their own risk assessments, whether it is of a potential medical diagnostic test or a particular consumer decision."--Publishers Weekly "Unbiased, comprehensible information on radiation risk is hard to come by...Strange Glowfills this gap."--Science "Narrative science at its best ... a propulsive story, each piece building on the next in a series of progressive revelations... A seismic piece of scientific inquiry, top shelf in narrative style and illumination."--Kirkus, starred review "The only antidote to irrational fear is knowledge, andStrange Glowimparts this in spades."--Independent "Strange Glow isn't about lessening what is largely a sensible fear, but about removing some of the mystery and misunderstanding... This is a long overdue and successful attempt to rationalise an emotional subject by telling its story in very human terms."--Engineering & Technology News "Strange Glow ... integrates detailed science and carefully illuminated medical statistics with the personal lives of scientists... The book's goal--'to present the facts about radiation as objectively and even-handedly as possible, leaving you to decide which aspects to fear'--is achieved with authority and style."--Andrew Robinson, Lancet "This book can be enjoyed as a sort of scientific QI--a string of interesting facts you can't wait to share with anyone who can be persuaded to listen... Jorgensen proves that there's no excuse for convoluted writing, however difficult the subject. His conversational style makes even the most complex equations seem attractive."--William Cook, The Spectator "[Strange Glow] is ... the story of human interaction with radiation--beginning with the one type that we can see (light) and continuing through radio waves, atomic blasts, cellphones, radon, microwave ovens, luggage scanners, the Fukushima accident, and on and on... Jorgensen avoids graphs and numbers, instead relying largely on entertaining--if alarming--anecdotes."--Nancy Szokan, Washington Post "What I certainly did not expect was to get caught up in the stories of the scientists [in Strange Glow]... Jorgensen has written a compelling book about the history of radiation... [His] gift is that he make us care about the scientists."--Jacqueline Cutler, Newark Star-Ledger "Strange Glow is a cracking good read, filled with fascinating stories about the people behind the science."--Literary Review "Timothy Jorgensen is a scientist with a knack for narrative storytelling."--Ryan Stellabotte, Fordham News "Strange Glow is clear, engaging and refreshingly willing to treat the reader as a thinking adult."--Japan Times "Strange Glow is a fantastic, well-written book about the benefits and risks of radiation. Jorgenson uses common prose so that a wide range of readers can follow the discussions...The book includes extensive, useful, and lucid discussions on medical x-rays and radon gas. Readers also learn the facts regarding the Three Mile Island, Chernobyl, and Fukushima nuclear reactor meltdowns and the problems they cause. [A] well-researched book."--Choice "Informative, fast paced and entertaining... I guarantee you will be engaged and surprised."--Chemistry World "Jorgensen's lucid writing and strong story-telling skills are demonstrated thoroughly in this book, making it a pleasure to read... In its simplicity and conciseness, it greatly contributes to removing some of the mystery and misunderstanding that surrounds radiation... I feel it will become a very useful resource to the general public as well as to radiation experts."--Jun Deng, Physics World "A narrative history, which integrates detailed science and statistics with the personal lives of the pioneers. [Jorgensen's] goal--'to present the facts about radiation as objectively and even-handedly as possible, leaving you to decide which aspects to fear'--is achieved with authority and style."--Andrew Robinson, The Telegraph "Jorgenson lays out the progression of mankind's understanding of radiation science over the past century, including the figures, breakthroughs and disasters that moved the field forward (for better or worse). An informative read that chronicles the history and science of humankind's 'ambivalent' relationship with this strange force."--Rachel Gross, Smithsonian "[Jorgensen] ... has chosen another original but interesting and straightforward way of storytelling, devoid of scientific jargon, to achieve the aim of reaching the widest possible audience of readers, regardless to their technical background... Strange Glow: The Story of Radiation ... Will certainly be useful due to the striking and exciting style of its presentation."--R.M. Alexakhin, Radiation Protection DosimetryTable of ContentsPREFACE ix 1. Nuclear Jaguars 1 PART ONE: RADIATION 101: THE BASICS 2. Now You See It: Radiation Revealed 7 3. Seek and You Shall Find: Radioactivity Everywhere 38 4. Splitting Hairs: Atomic Particles and Nuclear Fission 51 PART TWO: THE HEALTH EFFECTS OF RADIATION 5. Painted into a Corner: Radiation and Occupational Illness 81 6. The Hippocratic Paradox: Radiation Cures Cancer 116 7. Location, Location, Location: Radiation Sickness 141 8. Snow Warning: Radioactive Fallout 164 9. After the Dust Settles: Measuring the Cancer Risk of Radiation 187 10. Breeding Season: Genetic Effects 206 11. Crystal Clear: The Target for Radiation Damage 234 PART THREE: WEIGHING THE RISKS AND BENEFITS OF RADIATION 12. Silent Spring: Radon in Homes 275 13. A Tale of Two Cities: Diagnostic Radiography 293 14. Sorry, Wrong Number: Cell Phones 310 15. Hot Tuna: Radioactivity in Food 326 16. Blue Moon: Nuclear Power Plant Accidents 346 17. The Things They Carried: Geopolitical Radiation Threats 374 Epilogue: N-Rays 397 ACKNOWLEDGMENTS 407 NOTES AND CITATIONS 411 BIBLIOGRAPHY 453 INDEX 465
£27.00
Princeton University Press Strange Glow
Book SynopsisTrade ReviewWinner of the 2017 PROSE Award in History of Science, Medicine & Technology, Association of American Publishers One of Physics World's Top Ten Books of the Year, 2016 One of Choice's Outstanding Academic Titles for 2016 #6 on The Telegraph's Top 50 Books of the Year 2016 One of Smithsonian Magazine's Best Science Books of 2016 Shortlisted for Physics World's Book of the Year 2016 "Jorgensen walks readers through the history of humanity's interaction with radiation... [Strange Glow] is a solid, accessible work, but perhaps its most beneficial aspect is that Jorgensen equips readers with enough knowledge to make their own risk assessments, whether it is of a potential medical diagnostic test or a particular consumer decision."--Publishers Weekly "Unbiased, comprehensible information on radiation risk is hard to come by...Strange Glowfills this gap."--Science "Narrative science at its best ... a propulsive story, each piece building on the next in a series of progressive revelations... A seismic piece of scientific inquiry, top shelf in narrative style and illumination."--Kirkus, starred review "The only antidote to irrational fear is knowledge, andStrange Glowimparts this in spades."--Independent "Strange Glow isn't about lessening what is largely a sensible fear, but about removing some of the mystery and misunderstanding... This is a long overdue and successful attempt to rationalise an emotional subject by telling its story in very human terms."--Engineering & Technology News "Strange Glow ... integrates detailed science and carefully illuminated medical statistics with the personal lives of scientists... The book's goal--'to present the facts about radiation as objectively and even-handedly as possible, leaving you to decide which aspects to fear'--is achieved with authority and style."--Andrew Robinson, Lancet "This book can be enjoyed as a sort of scientific QI--a string of interesting facts you can't wait to share with anyone who can be persuaded to listen... Jorgensen proves that there's no excuse for convoluted writing, however difficult the subject. His conversational style makes even the most complex equations seem attractive."--William Cook, The Spectator "[Strange Glow] is ... the story of human interaction with radiation--beginning with the one type that we can see (light) and continuing through radio waves, atomic blasts, cellphones, radon, microwave ovens, luggage scanners, the Fukushima accident, and on and on... Jorgensen avoids graphs and numbers, instead relying largely on entertaining--if alarming--anecdotes."--Nancy Szokan, Washington Post "What I certainly did not expect was to get caught up in the stories of the scientists [in Strange Glow]... Jorgensen has written a compelling book about the history of radiation... [His] gift is that he make us care about the scientists."--Jacqueline Cutler, Newark Star-Ledger "Strange Glow is a cracking good read, filled with fascinating stories about the people behind the science."--Literary Review "Timothy Jorgensen is a scientist with a knack for narrative storytelling."--Ryan Stellabotte, Fordham News "Strange Glow is clear, engaging and refreshingly willing to treat the reader as a thinking adult."--Japan Times "Strange Glow is a fantastic, well-written book about the benefits and risks of radiation. Jorgenson uses common prose so that a wide range of readers can follow the discussions...The book includes extensive, useful, and lucid discussions on medical x-rays and radon gas. Readers also learn the facts regarding the Three Mile Island, Chernobyl, and Fukushima nuclear reactor meltdowns and the problems they cause. [A] well-researched book."--Choice "Informative, fast paced and entertaining... I guarantee you will be engaged and surprised."--Chemistry World "Jorgensen's lucid writing and strong story-telling skills are demonstrated thoroughly in this book, making it a pleasure to read... In its simplicity and conciseness, it greatly contributes to removing some of the mystery and misunderstanding that surrounds radiation... I feel it will become a very useful resource to the general public as well as to radiation experts."--Jun Deng, Physics World "A narrative history, which integrates detailed science and statistics with the personal lives of the pioneers. [Jorgensen's] goal--'to present the facts about radiation as objectively and even-handedly as possible, leaving you to decide which aspects to fear'--is achieved with authority and style."--Andrew Robinson, The Telegraph "Jorgenson lays out the progression of mankind's understanding of radiation science over the past century, including the figures, breakthroughs and disasters that moved the field forward (for better or worse). An informative read that chronicles the history and science of humankind's 'ambivalent' relationship with this strange force."--Rachel Gross, Smithsonian "[Jorgensen] ... has chosen another original but interesting and straightforward way of storytelling, devoid of scientific jargon, to achieve the aim of reaching the widest possible audience of readers, regardless to their technical background... Strange Glow: The Story of Radiation ... Will certainly be useful due to the striking and exciting style of its presentation."--R.M. Alexakhin, Radiation Protection DosimetryTable of ContentsPREFACE ix 1. Nuclear Jaguars 1 PART ONE: RADIATION 101: THE BASICS 2. Now You See It: Radiation Revealed 7 3. Seek and You Shall Find: Radioactivity Everywhere 38 4. Splitting Hairs: Atomic Particles and Nuclear Fission 51 PART TWO: THE HEALTH EFFECTS OF RADIATION 5. Painted into a Corner: Radiation and Occupational Illness 81 6. The Hippocratic Paradox: Radiation Cures Cancer 116 7. Location, Location, Location: Radiation Sickness 141 8. Snow Warning: Radioactive Fallout 164 9. After the Dust Settles: Measuring the Cancer Risk of Radiation 187 10. Breeding Season: Genetic Effects 206 11. Crystal Clear: The Target for Radiation Damage 234 PART THREE: WEIGHING THE RISKS AND BENEFITS OF RADIATION 12. Silent Spring: Radon in Homes 275 13. A Tale of Two Cities: Diagnostic Radiography 293 14. Sorry, Wrong Number: Cell Phones 310 15. Hot Tuna: Radioactivity in Food 326 16. Blue Moon: Nuclear Power Plant Accidents 346 17. The Things They Carried: Geopolitical Radiation Threats 374 Epilogue: N-Rays 397 ACKNOWLEDGMENTS 407 NOTES AND CITATIONS 411 BIBLIOGRAPHY 453 INDEX 465
£16.19
Princeton University Press The Molecular Switch
Book Synopsis
£74.80
Johns Hopkins University Press Genomic Medicine Articles from the New England
Book SynopsisEdited by leaders in the field, written by experts from around the globe, and brimming with full-color illustrations, Genomic Medicine is an indispensable guide to the full potential of the DNA-based transformation of medicine.Trade ReviewAn indispensable guide to the full potential of the DNA-based transformation of medicine. Human Reproduction and Genetic Ethics The editors have assembled a fine set of short, readable and highly authoritative articles by eminent authors. The book is beautifully produced and will give an accessible entry into genetic medicine to its target audience of non-specialist clinicians. Readers of this journal will need no persuading of the fascination and ever growing importance of genetic medicine and these themes are well communicated. -- Andrew P. Read Human Genetics 2004Table of ContentsForewordPrefaceList of Contributors GlossaryChapter 1. Getting Ready for Gene-Based MedicineChapter 2. Genomic Medicine – A PrimerChapter 3. Genetic TestingChapter 4. Population Screening in the Age of Genomic Medicine Chapter 5. Inheritance and Drug Response Chapter 6. Pharmacogenomics – Drug Disposition, Drug Targets, and Side EffectsChapter 7. Pharmacogenetics in the Laboratory and the Clinic Chapter 8. Hereditary Colorectal CancerChapter 9. Alzheimer's Disease and Parkinson's DiseaseChapter 10. Molecular Diagnosis of the Hematologic CancersChapter 11. Breast and Ovarian CancerChapter 12. Cardiovascular DiseaseChapter 13. Ethical, Legal, and Social Implications of Genomic MedicineChapter 14. Genomics as a Probe for Disease Biology Chapter 15. Welcome to the Genomic EraIndex
£29.70
Johns Hopkins University Press Geriatric Bioscience
Book SynopsisExamining the science of geriatrics and converting its concepts into clinical applications, this groundbreaking work provides clinicians and researchers with a solid foundation for a greater understanding of the diagnosis, treatment, and prevention of age-related diseases as well as recommendations for promoting healthy aging.Trade ReviewHamerman leads the advanced and careful reader to a better understanding of the relationships between the biology of aging and the emergence of aging-related diseases. Choice 2008 A unique presentation based on the author's many decades of research and experience in the science of aging. It is extremely well documented with more than a thousand references and a helpful reference... an important source of information from an expert. -- Dr. Steven Schwartz Riverdale Press 2008 The nexus between obesity, heart disease, diabetes, cancer, and Alzheimer's disease is tantalizing. It takes the hand of a master to synthesize so much material and give a unified overarching view of the relationship between aging and disease. This skillful hand is seen in the smooth flow of the book and a most pleasurable style of writing. -- David O. Staats Doody's Review Service 2008 A must-read for clinicians providing primary care in geriatrics -- Laura J. Niedernhofer Journal of Clinical Investigation 2008 An inspiring, concise, comprehensive, and stimulating primer of the field for aspiring gerontologists, geriatricians, and other physicians who will care for elderly persons. -- William B. Hazzard, MD JAMA 2008 The depth of knowledge presented is astounding... It is a must read for young and in-training geriatricians. -- Dr. Denise Zwahlen-Minton Pharos 2009 I found much of interest in the collection. -- John E. Morley Ageing and Society 2008Table of ContentsForeword by Robert N. ButlerPreface1. The Emergence and Future of Geriatrics2. An Introduction to Concepts of Molecular Biology3. AgingI. Trends in the Aging of the PopulationII. What Is Aging? Can We Distinguish betweenAging and Disease?III. Mechanisms of AgingIV. Longevity: Genetics of Extended Life Span or Risk for Adverse Events4. Interrelations of Certain Aging-related ConditionsI. Cytokines, Inflammation, and Responses to StressII. Atherosclerosis: Risk Factors for Cardiovascular DiseaseIII. The Metabolic Syndrome and Type 2 DiabetesIV. OsteoporosisV. OsteoarthritisVI. Frailty and Related Conditions: Anorexia, Sarcopenia, ExerciseVII. CancerVIII. Alzheimer Disease5. Interactive Therapies Significant for an Aging PopulationI. CyclooxygenasesII. StatinsIII. BisphosphonatesIV. Thiazolidinediones and Peroxisome Proliferatoractivated ReceptorV. Cytokine Inhibitors as a Landmark for Newer Anti-inflammatory TherapiesVI. Hormonal Therapies6. Preventive GerontologyI. The Compression or Expansion of Morbidity?II. Healthy Lifestyle PracticesIII. "Successful Aging"EpilogueGlossaryReferencesIndex
£38.70
Duke University Press Rendering Life Molecular
Book SynopsisNatasha Myers shows in this ethnography how scientists who build three-dimensional models of proteins use their senses and bodies to create, represent, and evaluate otherwise imperceptible molecules. These modelers often consider matter to be made up of living, moving, and sometimes breathing entities, and Myers' study of them rethinks the objectivity of science.Trade Review"Essential reading for those interested biopolitics, bioethics, science studies, and genetics, genomics, and the new omics." -- Rebecca Scott Yoshizawa * New Genetics and Society *"One of the most exciting books published this year, Natasha Myer’s Rendering Life Molecular: Models, Modelers, and Excitable Matter, is an ethnography of protein modellers, the first such study of this new and central area of biological research." -- Sherryl Vint * Year’s Work in Critical and Cultural Theory *"Amplifying instances of haptic and creative thinking opens avenues for a different kind of science than one may find presented in popular media.... The result is not only intellectually invigorating but also abounds in amusing curiosities. One might say 'eye opening,' but in the spirit of the book, it would perhaps be better to say 'vivifying.'" -- Jonathan G. Wald * Current Anthropology *"Rendering Life Molecular offers an engaging view into the world of scientists who describe the unseeable." -- R. M. Denome * Choice *"Rendering Life Molecular is a thought-provoking book, a whirlwind ethnography pregnant with epistemological and empirical insights on movements, practices, knowledge and reasoning around proteins, which can and should inform future philosophical studies of modeling as well as STS work on experimental practices in and beyond biology." -- Sabina Leonelli * Metascience *"... logical, theoretically and methodologically iterative, and, most importantly, ethnographically rich and robust." -- Udo Krautwurst * Journal of the Royal Anthropological Institute *Table of ContentsPreface ix Acknowledgments xiii Introduction 1 Part One. Laboratory Entanglements 1. Crystallographic Renderings 35 2. Tangible Media 74 3. Molecular Embodiments 99 Part Two. Ontics and Epistemics 4. Rending Representation 121 5. Remodeling Objectivity 136 Part Three. Forms of Life 6. Machinic Life 159 7. Lively Machines 182 8. Molecular Calisthenics 204 Conclusion: What Is Life Becoming? 230 Appendix: A Protein Primer 239 Notes 243 Bibliography 277 Index 299
£28.80
CABI Publishing Soybean
Book SynopsisSoybean is one of the world's leading sources of seed oil and seed protein and is grown extensively, particularly in the Americas and Asia. Indeed, soybean is the lowest-cost producer of vegetable oil, since the oil is a coproduct of its protein-rich meal. Major advances have recently been made in our understanding of soybean genetics and of the application of new technologies to soybean improvement. Thus it is now possible, using molecular methods, to alter the protein and oil composition of soybean, as well as produce other foreign proteins in the plant. Further progress should permit the improvement of nitrogen fixation and other desirable traits. This book focuses on recent progress in our understanding of the genetics and molecular biology of soybean and provides a broad review of the subject, from genome diversity to transformation and integration of desired genes using current technologies. It is aimed at workers in legume agronomy, plant genetics, breeding and biotechnology.Table of Contents1: Germplasm Diversity within Soybeans, R G Palmer, T Hymowitz and R LNelson 2: Molecular Genetic Mapping of Soybean, R C Shoemaker, K M Polzen and J ESpecht 3: Cytoplasmic Genetics in the Legumes (Fabaceae), with Special Reference toSoybean, S A Mackenzie 4: Plant Transposable Elements: Potential Application for Gene Tagging inSoybean, L O Vodkin 5: Limitations and Potentials of Genetic Manipulations of Soybean, J ESpecht and G L Graef 6: In vitro Selection and Culture-induced Variation in Soybean, J M Widholm 7: Soybean Seed Composition, N C Nielsen 8: Genetic Modification of Soybean Oil Quality, N S Yadav 9: Molecular Genetic Analysis of Soybean Nodulation Mutants, P M Gresshoff 10: Improvement of Soybean for Nitrogen Fixation: Molecular Genetics ofNodulation, A J Delauney and D P S Verma 11: Soybean Transformation: Technologies and Progress, J J Finer, T-S Chengand D P S Verma"
£116.68
CABI Publishing Testing Methods for SeedTransmitted Viruses
Book SynopsisThis practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.Table of ContentsPART I: Introduction 1: Seed transmission of viruses 2: Ecology, epidemiology and control PART II: Biological assays 3: Serological testing methods 4: Nucleic acid-based testing methods 5: Nucleic acid hybridization 6: Epilogue i: Appendix 1. List of seed-transmitted viruses and viroids ii: Appendix 2. Reagents, solutions and buffers iii: Appendix 3. Suppliers of laboratory equipment and materials
£92.31
CABI Publishing Hormonal Regulation of Farm Animal Growth
Book SynopsisThis book describes the cellular and molecular mechanisms that control farm animal growth, includingdevelopment and body composition. The emphasis is on circulating hormones, local growth factors andgene transcription factors which regulate growth and differentiation of skeletal muscle, bone andadipose tissue. Overall, this book will be an important resource for students that have a limitedbackground in cell, molecular and developmental biology and the effect of endocrine and growthfactors on the growth process.Table of Contents1: Whole Animal Growth 2: Cellular and Molecular Biology 3: The Endocrine System 4: Development of Muscle, Skeletal System and Adipose Tissue 5: Growth Hormone and Insulin-like Growth Factors 6: Calcium Homeostasis and Regulation of Bone Growth 7: Hormones, Growth Factors and Skeletal Muscle 8: Hormones, Growth Factors and Adipose Tissue 9: Steroids and Animal Growth 10: Catecholamines, b-agonists and Nutrient Partitioning 11: Leptin, Body Composition and Appetite Control
£44.56
CABI Publishing Biotechnology and Plant Genetic Resources
Book SynopsisAdvances in molecular and cell biology have led to the development of a whole range of techniques for manipulating genomes, collectively termed biotechnology. Although much of the focus in the plant sciences has been on the direct manipulation of plant genomes, biotechnology has also catalyzed a renewed emphasis on the importance of biological and genetic diversity and its conservation. The methods of biotechnology now permit a greater understanding of both species and genetic diversity in plants, the mechanisms by which that variation is generated in nature, and the significance of that variation in the adaptation of plants to their environment. They allow the development of rapid methods for screening germplasm for specific characters and promote more effective conservation strategies by defining the extent of genetic diversity. Tissue culture-based techniques are available for conserving germplasm that cannot be maintained by more traditional methods. Also sophisticated informatics Table of Contents1: An Overview J A Callow, B V Ford-Lloyd and H J Newbury 2: Use of molecular marker techniques for description of plant genetic variation A L Westman and S Kresovich 3: Genetic diversity - population structure and conservation M D Hayward and N R Sackville Hamilton 4: Genenomic relationships, conserved synteny and wide-hybrids D A Laurie, G J Bryan and J W Snape 5: Molecular markers and the management of genetic resources in seed genebanks: a case study of rice B V Ford-Lloyd, M T Jackson and H J Newbury 6: In vitro conservation methods F Engelmenn 7: Conservation of DNA: DNA banking R P Adams 8: Genetic resources and plant breeding M J Kearsey 9: Gene identification, isolation and transfer I D Godwin 10: Importance of biotechnology for germplasm health and quarantine H Barker and L Torrance 11: Biodiversity for bioindustries G Tamayo, W F Naider and A Sittenfeld 12: Internet resources for the biologist M L Anderson and S W Cartinhour
£116.68
John Wiley and Sons Ltd Molecular Evolution
Book SynopsisThe study of evolution at the molecular level has given the subject of evolutionary biology a new significance. Phylogenetic ''trees'' of gene sequences are a powerful tool for recovering evolutionary relationships among species, and can be used to answer a broad range of evolutionary and ecological questions. They are also beginning to permeate the medical sciences. In this book, the authors approach the study of molecular evolution with the phylogenetic tree as a central metaphor. This will equip students and professionals with the ability to see both the evolutionary relevance of molecular data, and the significance evolutionary theory has for molecular studies. The book is accessible yet sufficiently detailed and explicit so that the student can learn the mechanics of the procedures discussed. The book is intended for senior undergraduate and graduate students taking courses in molecular evolution/phylogenetic reconstruction. It will also be a useful supplement for students taking Table of ContentsThe Archaeology of the Genome. Trees; Genes: Organization, Function and Evolution. Genes in Populations. Measuring Genetic Change. Inferring Molecular Phylogeny. Models of Molecular Evolution. Applications of Molecular Phylogenetics
£85.46
Springer-Verlag New York Inc. Computer Simulations of Aggregation of Proteins and Peptides
Book SynopsisThis volume provides computational methods and reviews various aspects of computational studies of protein aggregation. Chapters discuss the relationship between protein misfolding and protein aggregation, methods of prediction of aggregation propensities of protein, peptides, protein structure, results of computer simulations of aggregation, and computational simulations focused on specific diseases such as Alzheimer''s, Parkinson''s, and preeclampsia. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Computer Simulations of Aggregation of Proteins and Peptides aims to ensure successful results in the further study of this vital field.Table of Contents1. Bioinformatics Methods in Predicting Amyloid Propensity of Peptides and Proteins Małgorzata Kotulska and Jakub W. Wojciechowski 2. Protocols for Rational Design of Protein Solubility and Aggregation Properties using Aggrescan3D Standalone Aleksander Kuriata, Aleksandra E. Badaczewska-Dawid, Jordi Pujols, Salvador Ventura, and Sebastian Kmiecik 3. Using Surface Hydrophobicity Together with Empirical Potentials to Identify Protein-Protein Binding Sites. Application to the Interactions of E-cadherins Robert L. Jernigan, Pranav Khade, Ambuj Kumar, and Andrzej Kloczkowski 4. Computational Models for Study of Protein Aggregation Nguyen Truong Co, Mai Suan Li, and Pawel Krupa 5. Probing Protein Aggregation Using the Coarse-Grained UNRES Force Field Ana V. Rojas, Gia G. Maisuradze, Harold A. Scheraga, and Adam Liwo 6. Contact-based Analysis of Aggregation of Intrinsically Disordered Proteins Marek Cieplak, Łukasz Mioduszewski, and Mateusz Chwastyk 7. Molecular Insights into the Effect of Metals on Amyloid Aggregation Yifat Miller 8. From Quantum Mechanics, Classical Mechanics and Bioinformatics to Artificial Intelligence Studies in Neurodegenerative Diseases Orkid Coskuner-Weber, M. Gokhan Habiboglu, David Teplow, and Vladimir N. Uversky 9. Computer Simulations Aimed at Exploring Protein Aggregation and Dissociation Phuong H. Nguyen and Philippe Derreumaux 10. All-atom Molecular Dynamics Simulation Methods for Aggregation of Protein and Peptides: Replica-exchange/permutation and Nonequilibrium Simulations Satoru G. Itoh and Hisashi Okumura 11. Key Factors Controlling Fibril Formation of Proteins Tran Thi Minh Thu, Andrzej Kloczkowski, Mai Suan Li, and Maksim Kouza 12. Determination of the Most Stable Packing Of Peptides From Ribosomal S1 Protein, Protein Bgl2p and Aβ peptide in β-layers during Molecular Dynamics Simulations Glyakina A.V., Balabaev N.K., and Galzitskaya O.V 13. Molecular Dynamics Simulations Of Protein Aggregation: Protocols For Simulation Setup and Analysis with Markov State Models And Transition Networks Suman Samantray, Wibke Schumann, Alexander-Maurice Illig, Arghadwip Paul, Bogdan Barz, and Birgit Strodel 14. Challenges in Experimental Methods Marlena E. Gąsior-Głogowska, Natalia Szulc, and Monika Szefczyk 15. Aggregates Sealed By Ions Giovanni La Penna and Silvia Morante 16. Modifying Amyloid Motif Aggregation through Local Structure Sofia Bali and Lukasz A. Joachimiak 17. Assessing the Stability Of Biological Fibrils By Molecular-Scale Simulations Rodrigo A. Moreira 18. Predictive Modeling of Neurotoxic α-Synuclein Polymorphs Liang Xu, Shayon Bhattacharya, and Damien Thompson 19. Characterization of Amyloidogenic Peptide Aggregability in Helical Subspace Shayon Bhattacharya, Liang Xu, and Damien Thompson 20. Exploration of Protein Aggregations in Parkinson’s Disease through Computational Approaches and Big Data Analytics Saba Shahzadi, Muhammad Yasir, Bisma Jawad, Sumbal Baber, Mubashir Hassan 21. Computational Studies of Protein Aggregation In Preeclampsia Maksim Kouza, Andrzej Kolinski, Irina Buhimschi, and Andrzej Kloczkowski 22. Final remarks Mai Suan Li, Andrzej Kloczkowski, Marek Cieplak, and Maksim Kouza
£143.99
Springer-Verlag New York Inc. Solanum tuberosum
Book SynopsisThis volume offers a comprehensive collection of experimental approaches to study Solanum tuberosum (potato). Chapters are divided into five sections covering a general overview of potato, the research fields of molecular biology, omics approaches, and bioinformatics, and finally, the application of the generated knowledge for crop improvement. Written in the format of the highly successful Methods in Molecular Biology series, each wet-lab chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and practical, Solanum tuberosum: Methods and Protocols aims to provide a collection of methods for the potato research community that will enable scientists gain insight into the world of the potato.Chapter 2 is available open access under a Creative ComTable of ContentsImportance of Potato as a Crop and Practical Approaches to Potato Breeding.- Cryopreservation of Potato Shoot Tips for Long Term Storage.- RNA Sequencing Analyses for Deciphering Potato Molecular Response.- Yeast-2-hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum.- Potato as a Model for Field Trials with Modified Gene Functions in Research And Translational Experiments.- DAP-seq Identification of Transcription Factor Binding Sites in Potato.- Mass Spectrometric Monitoring Of Plant Hormone Crosstalk During Biotic Stress Responses In Potato (Solanum tuberosum L.).- A Comprehensive Guide to Potato Transcriptome Assembly.- MapMan visualization of RNA-seq data using Mercator4 functional annotations.- Identification of Resistance Genes Using Diagnostic R-gene Enrichment Sequencing (dRenSeq).- Methodologies for Discovery and Quantitative Profiling of sRNAs in Potato.- Co-expression for Genotype-Phenotype Function Annotation In Potato Research.- Computer Vision and Less Complex Image Analyses to Monitor Potato Traits In Fields.- Quantifying the contribution to virulence of Phytophthora infestans effectors in potato.- Identification of Solanum immune receptors by Bulked Segregant RNA-Seq and high-Throughput Recombinant Screening.- Gene editing in potato using CRISPR-Cas9 technology.- Gene downregulation in potato roots using Agrobacterium rhizogenes-mediated Transformation.- Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato.- Towards the design of potato tolerant to abiotic stress.- Rapid loop-mediated isothermal amplification for detection of the Ralstonia solanacearum species complex bacteria in symptomatic potato tubers and plants.
£161.99
