Clinical and internal medicine Books

Table of ContentsPart I: Overview 1. Making Cardiomyocytes from Pluripotent Stem Cells Peter Karaginius and Yoshinori Yoshida Part II: Generation of Pluripotent Stem Cell-Derived Cardiomyocytes and Cardiac Tissues 2. A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells Yuika Morita, Shugo Tohyama, Jun Fujita, and Keiichi Fukuda 3. Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture Nagako Sougawa, Shigeru Miyagawa, and Yoshiki Sawa 4. Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells Emiko Ito, Shigeru Miyagawa, Yoshinori Yoshida, and Yoshiki Sawa 5. Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches Kenji Miki, Hirohide Saito, and Yoshinori Yoshida 6. Fabrication of Cardiac Constructs using bio-3D Printer Kenichi Arai, Daiki Murata, Shoko Takao, and Koichi Nakayama 7. Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium Junjun Li, Li Liu, Itsunari Minami, Shigeru Miyagawa, and Yoshiki Sawa 8. Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method Maki Takeda, Shigeru Miyagawa, Mitsuru Akashi, and Yoshiki Sawa 9. Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages Hidetoshi Masumoto Part III: Physiological Mesurements using Pluripotent Stem Cell-Derived Cardiomyocytes 10. Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes Jun LI and Jong-Kook LEE 11. Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies Tadashi Takaki and Yoshinori Yoshida 12. Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Daisuke Yoshinaga, Yimin Wuriyanghai, and Takeru Makiyama 13. Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai, Daisuke Yoshinaga, and Takeru Makiyama 14. Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells Misato Koakutsu, Tadashi Takaki, Kenji Miki, and Yoshinori Yoshida 15. Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis Yasunari Kanda, Ayano Satsuka, Sayo Hayashi, Mihoko Hagiwara-Nagasawa, and Atsushi Sugiyama 16. Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells Daisuke Sasaki, Katsuhisa Matsuura, and Tatsuya Shimizu 17. A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues Yuya Fujiwara, Kohei Deguchi, Kenji Miki, Tomoyuki Nishimoto, and Yoshinori Yoshida Part IV: Transcriptome and Bioinfomatics Analysis 18. Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart Manami Kotoh, Seitaro Nomura, Shintaro Yamada,Hiroyuki Aburatani, and Issei Komuro 19. RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes Chikako Okubo, Megumi Narita, Takuya Yamamoto, and Yoshinori Yoshida 20. Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells Antonio Lucena-Cacace and Yoshinori Yoshida Part V: Gene Editing and CRISPR Technology for Pluripotent Stem Cells 21. Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs) Shuichiro Higo, Shungo Hikoso, Shigeru Miyagawa, and Yasushi Sakata 22. Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9 Tatsuya Anzai, Hiromasa Hara, Chanthra Nawin, Taketaro Sadahiro, Masaki Ieda, Yutaka Hanazono, and Hideki Uosaki 23. CRISPRi/a Screening with Human iPSCs Masataka Nishiga, Lei S. Qi, and Joseph C. Wu Part VI: Transplantation of Pluripotent Stem Cell-Derived Cardiomyocytes 24. Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model Takeshi Hatani and Yoshinori Yoshida 25. Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey Hideki Kobayashi, Hajime Ichimura, Noburou Ohashi, and Yuji Shiba

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Book Synopsis* A clear and practical guide to one of the biggest challenges in primary care * Addresses the complex issues faced in treating and managing patients with medically unexplained symptoms'.Trade Review“The major contribution this monograph makes is to recognize that not all symptoms are readily explainable by a clear diagnosis. To young clinicians, this can be especially important as they face both the straightforward diagnosis and the conundrum. It is an easy read and another tool for working with primary care patients. ” (Doody’s, 30 August 2013) Table of ContentsContributors vii Acknowledgements ix 1 Introduction 1 Chris Burton 2 Epidemiology and Impact in Primary and Secondary Care 5 Alexandra Rolfe and Chris Burton 3 Considering Organic Disease 7 David Weller and Chris Burton 4 Considering Depression and Anxiety 10 Alan Carson and Jon Stone 5 Medically Unexplained Symptoms and the General Practitioner 15 Christopher Dowrick 6 Principles of Assessment and Treatment 18 Chris Burton 7 Palpitations Chest Pain and Breathlessness 22 Chris Burton 8 Headache 27 David P. Kernick 9 Gastrointestinal Symptoms: Functional Dyspepsia and Irritable Bowel Syndrome 31 Henriëtte E. van der Horst 10 Pelvic and Reproductive System Symptoms 36 Nur Amalina Che Bakri, Camille Busby-Earle, Robby Steel and Andrew W. Horne 11 Widespread Musculoskeletal Pain 40 Barbara Nicholl, John McBeth and Christian Mallen 12 Fatigue 43 Alison J. Wearden 13 Neurological Symptoms: Weakness Blackouts and Dizziness 47 Jon Stone and Alan Carson 14 Managing Medically Unexplained Symptoms in the Consultation 52 Avril F. Danczak 15 Cognitive Approaches to Treatment 56 Vincent Deary 16 Behavioural Approaches to Treatment 60 Vincent Deary 17 Pharmacological Treatment 64 Killian A. Welch 18 Conclusion 68 Chris Burton Appendix: Suggestions for Reflection and Audit 69 Chris Burton Index 71

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Book SynopsisA powerful new approach to natural, intuitive whole-body healing.The Body Code is a truly revolutionary method of holistic healing. Dr. Bradley Nelson, a globally renowned expert in bioenergetic medicine, has spent decades teaching his powerful self-healing method and training practitioners around the globe, but this is the first time his system of healing will be available to the general public in the form of The Body Code.The Body Code is based on the simple premise that the body is self-healing and knows what it needs in order to thrive and flourish. The Body Code method allows readers to tap into this inner knowing, and find imbalances in 6 key areasEnergies, Circuits and Systems, Toxicity, Nutrition and Lifestyle, Misalignments, and Pathogensthat are the root causes of our physical, mental and emotional issues. By identifying and releasing these imbalances, readers become empowered to activate their body''s innate healing power.

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Book SynopsisImprove your skills in visual diagnosis Speed and accuracy of diagnosis is the key to saving lives in emergency and critical care medicine. Careful visual inspection of the patient, the data (radiography, electrocardiogram), and related clues can often help providers choose the right diagnosis and ultimately the best treatment but this knowledge comes with experience. This book provides 110 randomly presented visual diagnosis cases for self-testing, imitating real-life situations found in the emergency department setting. Written by distinguished emergency and critical care physicians, and thoroughly revised and updated throughout, this second edition includes 25% new cases and is an ideal aid for trainees preparing for Board examinations as well as an invaluable refresher' for qualified emergency and critical care providers.This title is also available as a mobile App from MedHand Mobile Libraries. Buy it now from Trade Review“Image quality, a critical aspect of a work of this type, and succinct but clear presentations, make this book successful.” (Doody’s, 27 April 2012) "These discrepancies detract only slightly from the overall quality of the book, however, and it can be recommended for purchase by any ED library." (Emergency Nurse, 1 December 2011) Table of ContentsList of contributors ix Foreword xii Preface xiv Illustration credits xv Part I Case Presentations and Questions 1 1 Slash Wound to the Neck 3Kevin S. Barlotta MD and Alexander B. Baer MD 2 “I’ve Got Blood in My Eye” 3Chris S. Bergstrom MD and Alexander B. Baer MD 3 Forearm Fracture After Falling 4Alexander B. Baer MD 4 A Neonate with Fever and Rash 4David L. Eldridge MD 5 A Missing Button Battery 5Brendan G. Carr MD and Sarah E. Winters MD MSCE 6 Anorexia Hair Loss and Fingernail Bands 6Christopher P. Holstege MD 7 Wide Complex Tachycardia in a Young Adult 6William J. Brady MD 8 Wide Complex Tachycardia in an Older Male Patient 7William J. Brady MD 9 Muscle Spasms Following a Spider Bite 8J. Michael Kowalski DO and Adam K. Rowden DO 10 Necrotic Skin Lesion 8David A. Kasper DO MBA Aradhna Saxena MD and Kenneth A. Katz MD 11 Intense Pain Following High-pressure Injection Injury 9David T. Lawrence DO 12 Prenatal Vitamin Overdose 9Christopher P. Holstege MD and Adriana I. Goldberg MD 13 Blurred Vision Following Yard Work 10Allyson Kreshak MD 14 Foot Pain in a Gymnast 11Hoi K. Lee MD 15 A Child with Bruises of Different Ages 11David L. Eldridge MD 16 Sudden Shortness of Breath after Removal of a Central Line 12Christopher P. Holstege MD 17 “My Eyes Are Yellow!” 13David T. Lawrence DO 18 Pleuritic Chest Pain in a Young Adult Male 13William J. Brady MD 19 Exposed During a Blizzard 14Joseph D. Forrester MD and Christopher P. Holstege MD 20 FAST Evaluation of a Trauma Patient 15John S. Rajkumar MD and James H. Moak MD RDMS 21 Chest Pain with Sudden Cardiac Death 15William J. Brady MD 22 Wrist “Sprain” in a Child 16Jennifer S. Boyle PharmD MD 23 Acute Eye Pain and Blurred Vision in an Elderly Female 17Chris S. Bergstrom MD and Alexander Baer MD 24 Heel Pain Following a Fall 17Jennifer S. Boyle PharmD MD 25 Confluent Rash on a Child 18Sarah E. Winters MD MSCE and Brendan G. Carr MD 26 Bradycardia Following an Herbal Ingestion 18Alexander B. Baer MD 27 A Pain-free Adult with Persistent T Wave Abnormalities 19William J. Brady MD 28 Caustic Ingestion with Cardiotoxic Effects 20Heather A. Borek MD and Christopher P. Holstege MD 29 Chemical Eye Exposure 21Chris S. Bergstrom MD and Alexander B. Baer MD 30 Acute Abdominal Pain in Pregnancy 22James H. Moak MD RDMS and John S. Rajkumar MD 31 Coma Following Head Trauma 22Andrew L. Homer MD and William J. Brady MD 32 Tongue Swelling in a Hypertensive Female 23Kevin S. Barlotta MD and Alexander B. Baer MD 33 Purulent Eye Discharge in an Adult 24Chris S. Bergstrom MD and Alexander B. Baer MD 34 Shoulder Pain Following Direct Blow 24Nathan P. Charlton MD 35 A Gagging Child 25Maureen Chase MD and Worth W. Everett MD 36 Adult Male with a Sudden Severe Headache 25Andrew L. Homer MD and William J. Brady MD 37 New Facial Droop 26Andrew D. Perron MD and Christopher T. Bowe MD 38 Eye Pain After Tree Branch Strike 26Chris S. Bergstrom MD and Alexander B. Baer MD 39 An Elderly Woman with Groin Pain 27Brendan G. Carr MD 40 Rash and Joint Pain in a Child 28Mara L. Becker MD 41 Radiology Findings after Laparoscopy 28Munish Goyal MD 42 Postprandial Abdominal Pain in an Elderly Woman 29Hoi K. Lee MD 43 “Pink Eye” in a Contact Lens Wearer 30Chris S. Bergstrom MD and Alexander B. Baer MD 44 Suspicious Hand Pain 30Rex G. Mathew MD 45 Fever and Rash in a Child 31David L. Eldridge MD 46 An Alcoholic with Dyspnea 31James H. Moak MD RDMS and John S. Rajkumar MD 47 Dark Urine from an Immigrant 32Suzanne M. Shepherd MD 48 Chest Pain and Lead aVR ST Segment Elevation 33William J. Brady MD 49 Hand Pain after Striking a Wall 34William J. Brady MD and Kevin S. Barlotta MD 50 A Refugee with Skin Lesions 35Roger A. Band MD and Jeanmarie Perrone MD 51 Pain out of Proportion to Examination 35J. Michael Kowalski DO and Adam K. Rowden DO 52 Leg Pain Following a Motor Vehicle Collision 36Nathan P. Charlton MD 53 Deformed Globe Following Trauma 36Worth W. Everett MD 54 Foot Pain Following Breaking 37Munish Goyal MD 55 Lamp Oil Ingestion 38David L. Eldridge MD 56 Intermittent Abdominal Pain in a Female 38John S. Rajkumar MD and James H. Moak MD RDMS 57 Hallucinations in a Botanist 39Joseph D. Forrester MD and Christopher Holstege MD 58 Altered Mental Status with an Abnormal Electrocardiogram 40William J. Brady MD 59 Fishing in the Stomach 41Joseph D. Forrester MD and Christopher P. Holstege MD 60 Overdose-induced Boiled Lobster Skin 41Heather A. Borek MD and Christopher P. Holstege MD 61 Back Pain Following a Fall 42Andrew D. Perron MD and Christopher T. Bowe MD 62 Painful Facial Rash 42Chris S. Bergstrom MD and Alexander B. Baer MD 63 Intense Wrist Pain Following Trauma 43Rex G. Mathew MD 64 Fever and Drooling in a Child 44Sarah E. Winters MD MSCE and Brendan G. Carr MD 65 Syncope and Flank Pain in an Elderly Man 44John S. Rajkumar MD and James H. Moak MD RDMS 66 Get Them Undressed! 45Munish Goyal MD 67 A “Blue Hue” Following Endoscopy 46Saumil Vaghela PharmD and Christopher P. Holstege MD 68 Acute-onset Blurred Vision 46Chris S. Bergstrom MD and Alexander B. Baer MD 69 Elbow Pain in a Child After a Fall 47Elizabeth Cochran Ward MD and Alexander B. Baer MD 70 Confusion Anemia and Abdominal Pain in a Toddler 47Christopher P. Holstege MD and Joseph T. Vance 71 A Ground-level Fall with Ankle Pain 48Christopher T. Bowe MD 72 Traumatic Eye Pain and Proptosis 49Chris Bergstrom MD and Alexander Baer MD 73 Diffuse Ankle Pain Following a Fall 49Andrew D. Perron MD and Christopher T. Bowe MD 74 Rash Following Brush Fire 50Christopher P. Holstege MD and Alejandro C. Stella MD 75 Abdominal Pain in a Trauma Victim 51Esther H. Chen MD 76 Skin Target Lesion 51Mara L. Becker MD 77 Chest Pain and a Confounding Electrocardiogram Pattern 52William J. Brady MD 78 Sudden Sedation in a Student 53David T. Lawrence DO 79 Skin Lesions in a Comatose Patient 53Christopher P. Holstege MD and Matthew D. Wilson MD 80 Raccoon Eyes 54Angela M. Mills MD 81 Fall on an Outstretched Hand in a Young Adolescent 55William Brady MD and Kevin S. Barlotta MD 82 Eye Pain and Facial Swelling 55Adam K. Rowden DO and Chris S. Bergstrom MD 83 Wrist Pain Following Fall on an Outstretched Hand 56William J. Brady MD and Kevin S. Barlotta MD 84 Rash on a Child with Epilepsy 56Heather A. Borek MD and Christopher P. Holstege MD 85 Abdominal Pain in an Alcoholic 57Angela M. Mills MD 86 Chest Pain with Electrocardiographic ST Segment and T Wave Abnormalities 58William J. Brady MD 87 A Heroin Abuser with Multiple Skin Lesions 58Christopher P. Holstege MD and Ashley L. Harvin MD 88 Chest Pain in a Middle-aged Male Patient with ST Segment Elevation 59William J. Brady MD 89 Fire Victim with Hoarseness 60Kathryn Mutter MD and Christopher P. Holstege MD 90 A Gardener with a Non-healing Rash 60Roger A. Band MD and Steve Larson MD 91 A Bite to the Leg in Tall Grass 61Alejandro C. Stella MD and Christopher P. Holstege MD 92 An Elderly Man with Diffuse Facial Edema 62Kevin S. Barlotta MD and Alexander B. Baer MD 93 Acute-onset Double Vision 62Chris S. Bergstrom MD and Alexander B. Baer MD 94 Low Back Pain in a Car Accident Victim 63Edward G. Walsh MD and William J. Brady MD 95 Pain and Rash Following Contact with a Caterpillar 64Nathan P. Charlton MD and Mairin Smith MD 96 Moonshine-induced Basal Ganglion Necrosis and Metabolic Acidosis 64Nathan P. Charlton MD and Christopher P. Holstege MD 97 A Rock Climber with Finger Pain Swelling and Redness 65Joseph D. Forrester MD and Christopher P. Holstege MD 98 Vomiting and Syncope Following Ingestion of Ramps 66Christopher P. Holstege MD and Justin H. Price MD 99 Chest Pain and Subtle ST Segment Elevation 66William J. Brady MD 100 Herbalist with Bradycardia and Vision Changes 67William J. Brady MD 101 Painless Penile Ulcer 68Andrea L. Neimann MD 102 Hyperthermia Autonomic Instability and Confusion in a Traveler 68Christopher P. Holstege MD and Alexander B. Baer MD 103 An Immigrant with Neck Swelling 69Suzanne M. Shepherd MD and William H. Shoff MD 104 Eyelid Laceration Following a Brawl 70Chris S. Bergstrom MD and Alexander B. Baer MD 105 Young Athlete with Back Pain 71Edward G. Walsh MD and William J. Brady MD 106 Chest Pain and Hypotension in an Adult Male Patient 71William J. Brady MD 107 Adult Male with Atraumatic Lower Back Pain and Leg Weakness 72William J. Brady MD 108 Facial Swelling in a Patient with Poor Dentition 73Alexander B. Baer MD and Christopher P. Holstege MD 109 Weakness and Bradycardia in an Elderly Female Patient 73William J. Brady MD 110 Lightning Strike Induced Skin Changes 74Christopher P. Holstege MD Part II Answers Diagnoses and Discussion 75 Index 183

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Book SynopsisPathophysiology of Peptic Ulcer Disease.- Decreased Intragastric Acid Concentration as an Approach to Peptic Disease Therapy.- General Approach to Peptic Disease Therapy.- Ulcer Healing by Strengthening of Mucosal Defense.- History of the Development of Sucralfate.- Sucralfate: A Nonsystemic Site Protective Agent.- Chemistry of Sucralfate.- Binding of Bile Acids by Sucralfate.- Mechanisms of Action of Sucralfate.- Binding of Sucralfate to the Mucosal Surface.- Stimulation of Mucus Production.- Gastroduodenal Bicarbonate Secretion and its Response to Sucralfate.- Effect of Sucrose Octasulfate on Isolated Gastric Cells.- Effect on Gastric Surfactant.- Stimulation of Mucosal Prostaglandins by Sucralfate.- Sucralfate and Helicobacter pylori.- Sucralfate and Cell Proliferation.- Vascular Factors.- Sucralfate.- Effects of Sucralfate on Growth Factor Availability.- Duodenal Ulcer Therapy, Acid Rebound, and Early Relapse.- Effect of Sucralfate on Experimental Ulcers.- Effect on Experimental EsTable of ContentsIntroduction; D. Hollander, G.N.J. Tytgat. Pathophysiology of Peptic Ulcer Disease; F. Halter. Decreased Intragastric Acid Concentration as an Approach to Peptic Disease Therapy; A.B.R. Thomson. Strengthening of Mucosal Defenses as an Approach to Peptic Disease Therapy; A.S. Tarnawski. History of the Development of Sucralfate; A. Ishimori. The Chemistry of Sucralfate; K. Ochi. Binding of Bile Acids by Sucralfate; W.F. Caspary. Binding of Sucralfate to the Mucosal Surface; G. Morris. Stimulation of Mucus Production by Sucralfate; C. TasmanJones. Stimulation of Gastroduodenal Bicarbonate Secretion by Sucralfate; D.W. Rees. Effect of Sucrose Octasulphate on Isolated Gastric Cells; M. Lucey, T. Yamada. Effect of Sucralfate of Gastric Surfactant; B.A. Hills. Stimulation of Mucosal Prostaglandins by Sucralfate; D. Rachmilewitz. Effect of Sucralfate on Helicobacter Pylori; J.A. Louw. Sucralfate and Cell Proliferation; H. Kuwayama. Effect of Sucralfate on Vascular Factors: Mucosal Vasoprotection and Angiogenesis; S. Szabo. Sucralfate Effects on Growth Factor Availability; J. Konturek. 16 additional articles. Index.

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