Description
Table of ContentsPart I: Overview
1. Making Cardiomyocytes from Pluripotent Stem Cells
Peter Karaginius and Yoshinori Yoshida
Part II: Generation of Pluripotent Stem Cell-Derived Cardiomyocytes and Cardiac Tissues
2. A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells
Yuika Morita, Shugo Tohyama, Jun Fujita, and Keiichi Fukuda
3. Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture
Nagako Sougawa, Shigeru Miyagawa, and Yoshiki Sawa
4. Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells
Emiko Ito, Shigeru Miyagawa, Yoshinori Yoshida, and Yoshiki Sawa
5. Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches
Kenji Miki, Hirohide Saito, and Yoshinori Yoshida
6. Fabrication of Cardiac Constructs using bio-3D Printer
Kenichi Arai, Daiki Murata, Shoko Takao, and Koichi Nakayama
7. Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium
Junjun Li, Li Liu, Itsunari Minami, Shigeru Miyagawa, and Yoshiki Sawa
8. Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method
Maki Takeda, Shigeru Miyagawa, Mitsuru Akashi, and Yoshiki Sawa
9. Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages
Hidetoshi Masumoto
Part III: Physiological Mesurements using Pluripotent Stem Cell-Derived Cardiomyocytes
10. Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes
Jun LI and Jong-Kook LEE
11. Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies
Tadashi Takaki and Yoshinori Yoshida
12. Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
Daisuke Yoshinaga, Yimin Wuriyanghai, and Takeru Makiyama
13. Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique
Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai, Daisuke Yoshinaga, and Takeru Makiyama
14. Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells
Misato Koakutsu, Tadashi Takaki, Kenji Miki, and Yoshinori Yoshida
15. Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis
Yasunari Kanda, Ayano Satsuka, Sayo Hayashi, Mihoko Hagiwara-Nagasawa, and Atsushi Sugiyama
16. Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells
Daisuke Sasaki, Katsuhisa Matsuura, and Tatsuya Shimizu
17. A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues
Yuya Fujiwara, Kohei Deguchi, Kenji Miki, Tomoyuki Nishimoto, and Yoshinori Yoshida
Part IV: Transcriptome and Bioinfomatics Analysis
18. Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart
Manami Kotoh, Seitaro Nomura, Shintaro Yamada,Hiroyuki Aburatani, and Issei Komuro
19. RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes
Chikako Okubo, Megumi Narita, Takuya Yamamoto, and Yoshinori Yoshida
20. Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells
Antonio Lucena-Cacace and Yoshinori Yoshida
Part V: Gene Editing and CRISPR Technology for Pluripotent Stem Cells
21. Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs)
Shuichiro Higo, Shungo Hikoso, Shigeru Miyagawa, and Yasushi Sakata
22. Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9
Tatsuya Anzai, Hiromasa Hara, Chanthra Nawin, Taketaro Sadahiro, Masaki Ieda, Yutaka Hanazono, and Hideki Uosaki
23. CRISPRi/a Screening with Human iPSCs
Masataka Nishiga, Lei S. Qi, and Joseph C. Wu
Part VI: Transplantation of Pluripotent Stem Cell-Derived Cardiomyocytes
24. Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model
Takeshi Hatani and Yoshinori Yoshida
25. Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey
Hideki Kobayashi, Hajime Ichimura, Noburou Ohashi, and Yuji Shiba
