Clinical and internal medicine Books

Table of ContentsPart I: Overview 1. Making Cardiomyocytes from Pluripotent Stem Cells Peter Karaginius and Yoshinori Yoshida Part II: Generation of Pluripotent Stem Cell-Derived Cardiomyocytes and Cardiac Tissues 2. A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells Yuika Morita, Shugo Tohyama, Jun Fujita, and Keiichi Fukuda 3. Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture Nagako Sougawa, Shigeru Miyagawa, and Yoshiki Sawa 4. Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells Emiko Ito, Shigeru Miyagawa, Yoshinori Yoshida, and Yoshiki Sawa 5. Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches Kenji Miki, Hirohide Saito, and Yoshinori Yoshida 6. Fabrication of Cardiac Constructs using bio-3D Printer Kenichi Arai, Daiki Murata, Shoko Takao, and Koichi Nakayama 7. Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium Junjun Li, Li Liu, Itsunari Minami, Shigeru Miyagawa, and Yoshiki Sawa 8. Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method Maki Takeda, Shigeru Miyagawa, Mitsuru Akashi, and Yoshiki Sawa 9. Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages Hidetoshi Masumoto Part III: Physiological Mesurements using Pluripotent Stem Cell-Derived Cardiomyocytes 10. Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes Jun LI and Jong-Kook LEE 11. Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies Tadashi Takaki and Yoshinori Yoshida 12. Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Daisuke Yoshinaga, Yimin Wuriyanghai, and Takeru Makiyama 13. Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai, Daisuke Yoshinaga, and Takeru Makiyama 14. Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells Misato Koakutsu, Tadashi Takaki, Kenji Miki, and Yoshinori Yoshida 15. Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis Yasunari Kanda, Ayano Satsuka, Sayo Hayashi, Mihoko Hagiwara-Nagasawa, and Atsushi Sugiyama 16. Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells Daisuke Sasaki, Katsuhisa Matsuura, and Tatsuya Shimizu 17. A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues Yuya Fujiwara, Kohei Deguchi, Kenji Miki, Tomoyuki Nishimoto, and Yoshinori Yoshida Part IV: Transcriptome and Bioinfomatics Analysis 18. Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart Manami Kotoh, Seitaro Nomura, Shintaro Yamada,Hiroyuki Aburatani, and Issei Komuro 19. RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes Chikako Okubo, Megumi Narita, Takuya Yamamoto, and Yoshinori Yoshida 20. Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells Antonio Lucena-Cacace and Yoshinori Yoshida Part V: Gene Editing and CRISPR Technology for Pluripotent Stem Cells 21. Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs) Shuichiro Higo, Shungo Hikoso, Shigeru Miyagawa, and Yasushi Sakata 22. Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9 Tatsuya Anzai, Hiromasa Hara, Chanthra Nawin, Taketaro Sadahiro, Masaki Ieda, Yutaka Hanazono, and Hideki Uosaki 23. CRISPRi/a Screening with Human iPSCs Masataka Nishiga, Lei S. Qi, and Joseph C. Wu Part VI: Transplantation of Pluripotent Stem Cell-Derived Cardiomyocytes 24. Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model Takeshi Hatani and Yoshinori Yoshida 25. Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey Hideki Kobayashi, Hajime Ichimura, Noburou Ohashi, and Yuji Shiba

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Book Synopsis* A clear and practical guide to one of the biggest challenges in primary care * Addresses the complex issues faced in treating and managing patients with medically unexplained symptoms'.Trade Review“The major contribution this monograph makes is to recognize that not all symptoms are readily explainable by a clear diagnosis. To young clinicians, this can be especially important as they face both the straightforward diagnosis and the conundrum. It is an easy read and another tool for working with primary care patients. ” (Doody’s, 30 August 2013) Table of ContentsContributors vii Acknowledgements ix 1 Introduction 1 Chris Burton 2 Epidemiology and Impact in Primary and Secondary Care 5 Alexandra Rolfe and Chris Burton 3 Considering Organic Disease 7 David Weller and Chris Burton 4 Considering Depression and Anxiety 10 Alan Carson and Jon Stone 5 Medically Unexplained Symptoms and the General Practitioner 15 Christopher Dowrick 6 Principles of Assessment and Treatment 18 Chris Burton 7 Palpitations Chest Pain and Breathlessness 22 Chris Burton 8 Headache 27 David P. Kernick 9 Gastrointestinal Symptoms: Functional Dyspepsia and Irritable Bowel Syndrome 31 Henriëtte E. van der Horst 10 Pelvic and Reproductive System Symptoms 36 Nur Amalina Che Bakri, Camille Busby-Earle, Robby Steel and Andrew W. Horne 11 Widespread Musculoskeletal Pain 40 Barbara Nicholl, John McBeth and Christian Mallen 12 Fatigue 43 Alison J. Wearden 13 Neurological Symptoms: Weakness Blackouts and Dizziness 47 Jon Stone and Alan Carson 14 Managing Medically Unexplained Symptoms in the Consultation 52 Avril F. Danczak 15 Cognitive Approaches to Treatment 56 Vincent Deary 16 Behavioural Approaches to Treatment 60 Vincent Deary 17 Pharmacological Treatment 64 Killian A. Welch 18 Conclusion 68 Chris Burton Appendix: Suggestions for Reflection and Audit 69 Chris Burton Index 71

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Book SynopsisA powerful new approach to natural, intuitive whole-body healing.The Body Code is a truly revolutionary method of holistic healing. Dr. Bradley Nelson, a globally renowned expert in bioenergetic medicine, has spent decades teaching his powerful self-healing method and training practitioners around the globe, but this is the first time his system of healing will be available to the general public in the form of The Body Code.The Body Code is based on the simple premise that the body is self-healing and knows what it needs in order to thrive and flourish. The Body Code method allows readers to tap into this inner knowing, and find imbalances in 6 key areasEnergies, Circuits and Systems, Toxicity, Nutrition and Lifestyle, Misalignments, and Pathogensthat are the root causes of our physical, mental and emotional issues. By identifying and releasing these imbalances, readers become empowered to activate their body''s innate healing power.

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