Chromatography Books

Book SynopsisChromatography is a technique for analyzing or separating mixtures. The first edition of Chromatography: Concepts and Contrasts, published in 1988, was one of the first books to discuss all the different types of chromatography under one cover and is still very popular today.Table of ContentsPreface to Second Edition. Acknowledgments. Preface to the First Edition. Symbols, Abbreviations, and Acronyms. 1 Impact of Industrial and Government Regulatory Practices on Analytical Chromatography. 1.1 Locus of Chromatography in Chemical Industry. 1.2 Governmental Organizations. 1.3 Nongovernmental Agencies. 1.4 Standards, Calibration, and NIST. 1.5 USP and Other Pharmacopoeias. 1.6 International Guidelines for Analytical Laboratories. 1.7 Final Comments. References. 2 Introduction to Chromatography. 2.1 Brief History. 2.2 Definitions and Classifications. 2.3 Summary. References. 3 Band Broadening and Kinetics. 3.1 Configurations of the Stationary Phase. 3.2 Rate Theory. References. 4 Physical Forces and Interactions. 4.1 Intermolecular and Interionic Forces. 4.2 Size Exclusion—Molecular Sieving. 4.3 Some Models. References. 5 Optimization and the Achievement of Separation. 5.1 Kinetics and Zone Broadening. 5.2 Thermodynamics and Zone Migration. 5.2 Achievement of Separation. 5.4 Optimization of Separations. References. 6 Comparisons Between Chromatographic Modes. 6.1 Gas Chromatography Compared to Liquid Chromatography. 6.2 Supercritical Fluids and Supercritical Fluid Chromatography. 6.3 Reduced Parameters. 6.4 Columnar and Planar Configurations. References. 7 Gas Chromatography. 7.1 Early History, Theories, and Classifications. 7.2 Instrumentation for Capillary GC. 7.3 Instrumentation for Packed-Column GC. 7.4 Stationary Phase. 7.5 Temperature Effects. 7.6 Special Topics. 7.7 Summary and Evaluation. References. 8 Liquid Chromatography in Columns. 8.1 Introductory Classifications. 8.2 Classification of HPLC Modes. 8.3 Instrumentation. 8.4 Reversed-Phase Method Development and Optimization. 8.5 RP-HPLC Alternatives for the Pharmaceutical Industry. 8.6 Preparative LC. 8.7 Special Topics. 8.8 Summary and Evaluation. References. 9 Quantitation: Detectors and Methods. 9.1 Detectors. 9.2 Data Acquisition and Processing. 9.3 Quantitative Analysis. References. 10 Chromatography with Mass Spectral Detection (GC/MS and LC/MS). 10.1 Basics of Mass Spectrometry. 10.2 Gas ChromatographyrMass Spectrometry. 10.3 Liquid ChromatographyrMass Spectrometry. 10.4 Other Hyphenated Methods. 10.5 Summary. References. 11 Liquid Chromatography on Plane Surfaces. 11.1 Paper Chromatography. 11.2 Thin-Layer Chromatography. 11.3 Other Topics. 11.4 Literature Summary and Applications. References. 12 Qualitative Analysis. 12.1 Retention Parameters. 12.2 Other Methods of Qualitative Analysis. References. 13 Capillary Electrophoresis and Capillary Electrochromatography 365 13.1 Principles of Electrophoresis. 13.2 Zone Electrophoresis. 13.3 Capillary Electrophoresis. 13.4 Capillary Electrochromatography. References. 14 Sample Preparation. 14.1 Extraction. 14.2 Dialysis. 14.3 Derivatization. References. 15 Special Applications. 15.1 Multidimensional Chromatography. 15.2 Biological Applications. 15.3 Chiral Separations. 15.4 Other Topics. References. 16 Selection of a Method. 16.1 Methods of Attack. 16.2 The Internet. 16.3 Experimental Approach. 16.4 Summary. References. Some Internet Web Sites of Interest to Chromatographers. Appendix A ICH Glossary. Appendix B. Index.

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Book SynopsisThis revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.Table of ContentsGetting Started. Basics of Separation. Detection Sensitivity and Selectivity. Sample Preparation. The Column. Non-Ionic Samples: Reversed- and Normal-Phase HPLC. Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC. Gradient Elution. Systematic Approach to the Reversed-Phase Separation of Regular Samples. Computer-Assisted Method Development. Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds. Chiral Separations. Preparative HPLC Separation. Quantitation (Including Trace Analysis). Completing the Method: Validation and Transfer. Appendices. Index.

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Book SynopsisIn the arena of new drug development, the rate of sample generation far exceeds the rate of sample analysis. LC/MS is an analytical tool that helps the researcher select the most promising sample for scrutiny, accelerating the discovery of new drugs. This book is the first to describe in detail the procedures and benefits of LC/MS applications.Trade Review"…a comprehensive primer and will appeal to laboratory scientists and instructors in the pharmaceutical field…" (Analytical Chemistry, November 2002) "...describes...applications in each phase of drug development...provides perspective on changes in strategies for pharmaceutical analysis..." (SciTech Book News, Vol. 26, No. 2, June 2002) "...useful not only for students and scholars of analytical chemistry, but also for managers and other scientists...another fine addition to the Wiley-InterScience Series on Mass Spectrometry, it should be in the collections of all research libraries." (The Chemist, Summer 2003)Table of ContentsPreface ix Acknowledgments xi 1. Introduction 1 Emerging Analytical Needs 1 Integration of LC/MS into Drug Development 3 Partnerships and Acceptance 6 Overview 10 2. Drug Development Overview 11 Analysis Perspectives 11 The Four Stages of Drug Development 12 Drug Discovery 14 Preclinical Development 15 Clinical Development 16 Manufacturing 17 3. Accelerated Drug Development 19 Accelerated Development Strategies 20 Quantitative and Qualitative Process Elements 20 Quantitative Process Pipeline 24 Qualitative Process Pipeline 25 Motivating Factors 27 Analysis Opportunities for Accelerated Development 28 Full-Time Equivalent 28 Sample Throughput Model 29 Elimination Model 29 Rate-Determining Event Model 31 Accelerated Development Perspectives 33 4. LC/MS Development 34 The Elements of LC/MS Application 34 HPLC 35 Mass Spectrometry 35 LC/MS Interface 36 LC/MS Growth 38 5. Strategies 41 Standard Methods 43 Template Structure Identification 46 Databases 49 Screening 50 Integration 53 Miniaturization 55 Parallel Processing 56 Visualization 58 Automation 61 Summary 63 6. LC/MS Applications 65 Drug Discovery 65 Proteomics 68 Protein Expression Profiling 70 Quantitation 76 Glycoprotein Mapping 78 Natural Products Dereplication 83 Lead Identification Screening 88 Bioaffinity Screening 89 Combinatorial Library Screening 92 Open-Access LC/MS 96 Structure Confirmation 97 High Throughput 100 Purification 102 Combinatorial Mixture Screening 103 In Vivo Drug Screening 106 Pharmacokinetics 109 In Vitro Drug Screening 115 Metabolic Stability Screening 118 Membrane Permeability 119 Drug-Drug Interaction 121 Metabolite Identification 122 Preclinical Development 123 Metabolite Identification 125 Impurity Identification 132 Degradant Identification 140 Clinical Development 145 Quantitative Bioanalysis—Selected Ion Monitoring 148 Quantitative Bioanalysis—Selected Reaction Monitoring 152 Quantitative Bioanalysis—Automated Solid-Phase Extraction 156 Quantitative Bioanalysis—Automated On-Line Extraction 162 Metabolite Identification 165 Degradant Identification 168 Manufacturing 171 Impurity Identification Using Data-Dependent Analysis 173 Peptide Mapping in Quality Control 176 Patent Protection 178 7. Future Applications and Prospects 183 Workstations 183 Multidimensional Analysis 186 Miniaturization 187 Information Management 189 Strategic Outsourcing 190 Summary 191 8. Perspectives on the Future Growth of LC/MS 192 9. Conclusions 195 Glossary 197 References 205 Index 235

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Book SynopsisDealing with the principles of calibration--both the theoretical and mathematical constructs which relate features of calibration equations to the physical phenomena that affect instruments and samples used on generating information.Table of ContentsPrinciples of Calibration. Calibration Practice. Prediction. Appendices. Index.

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Book SynopsisA comprehensive manual for the analyst (or chromatographer) for evaluating and using the various tandem systems obtained by combining different methods of chromatography and spectroscopy. It introduces the reader to the different separation techniques that can be combined with spectroscopic techniques.Table of ContentsPartial table of contents: INTRODUCTION TO TANDEM SYSTEMS. Identification Techniques for Tandem Use. Interface Conduits. GAS CHROMATOGRAPHY TANDEM SYSTEMS. Gas Chromatography IR Spectroscopy (GC/IR) Tandem Systems. Gas Chromatography/Atomic Spectroscopy (GC/AS) Tandem Systems. LIQUID CHROMATOGRAPHY TANDEM SYSTEMS. LC/IR Tandem Systems. Liquid Chromatography/Atomic Spectroscopy (LC/AS) Tandem Systems. OTHER TANDEM SYSTEMS. Thin Layer Chromatography/Spectroscopy Tandem Systems. Electrophoresis/Spectroscopy Tandem Systems. Index.

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Book SynopsisFocusing on what has been one of the driving forces behind the development of lab-on-a-chip devices, Separation Methods in Microanalytical Systems explores the implementation, realization, and operation of separation techniques and related complex workflows on microfabricated devices. The book details the design, manufacture, and integration of diverse components needed to perform an entire analytical procedure on a single miniaturized device. The content applies to a diversity of disciplines including chemical analysis, biomedical diagnostics, environmental monitoring, and drug discovery.Separation Methods in Microanalytical Systems lays its theoretical background in a way that scientists from varied disciplines can approach. The book describes factors that influence the performance of separation, such as microfluidic handling, sample pre-treatment, and detection. It also conveys fabrication and material issues, design challenges, and practical considerations. Trade Review“This is just what I have been looking for. To my knowledge it’s the first time that anyone has put together a definitive book on the subject... The editors have done an excellent job in ensuring that the authors keep to their brief and do not cover data or examples give by other authors, but [still] have allowed the authors their individual styles... it is [of] great value, a book every researcher and student should have.”— P. Myers, in Chromatographia, 2006, vol. 63Table of ContentsAnalytical Microsystems: A Bird’s Eye View. Microfluidics: Some Basics. Microfabrication and Integration. New Tools: Scalar Imaging, Velocimetry, and Simulation. Transport Modes: Realizations and Practical Considerations. Pressure-Driven Separation Methods on a Chip. Transverse Transport in Microsystems: Theory and Applications. Electro-Driven Separation Methods on Chips. Gas Chromatography on Microchips. Sample Preparation on Microchips. Detection on Microchips: Principles, Challenges, Hyphenation, and Integration. Analytical Applications on Microchips. Index

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Book SynopsisDriven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomics research. Written as a compilation of hands-on methods exemplified by the work of several recognized leaders in the field, this book may serve as a guide for selection of the optimal separation strategies to solve particular biological problems. Recent progress in the development of robust analytical techniques and instrumentation has created the need for good quality biological samples that are subject to analysis. Emphasizing the importance of sample preparation, the book explains how proteomes can be divided into smaller, less complicated subproteomes' for individual analysis. It also highlights several hybrid approaches that taTable of ContentsSample Preparation. Sample Prefractionation and Analyses. Applications of Electrophoresis in Proteomics. Applications of High-Performance Liquid Chromatography. Related Techniques.

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Book SynopsisTheAdvances in Chromatographyseriesprovides the most up-to-date information on a wide range of developments in chromatographic methods and applications. For more than five decades, scientists and researchers have relied upon this series to cover the state of the art in separation science. With contributions from among the leading researchers around the world, this respected series continues to present timely, cutting-edge reviews in the fields of bio-, analytical, organic, polymer, and pharmaceutical chemistry. With contributions from leading authorities, this is an enriching guide for analytical, organic, inorganic, clinical, and physical chemists; chromatographers; biochemists and biotechnologists; scientists in Academia, government, hospitals and industry in both research and quality control. This is Volume 27 originally published in 1987.Table of ContentsPhysicochemical and Analytical Aspects of the Adsorption Phenomena, HPLC in Endocrinology, Chiral Stationary Phases for the Direct LC Separation of Enantiomers, The Use of Modified Silica Gels in TLC and HPTLC, Micellar Liquid Chromatography, Derivatization in Liquid Chromatography, Analytical High-Performance Affinity Chromatography, Characterization of Unsaturated Aliphatic Compounds by GC/Mass Spectrometry.

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Book SynopsisTheAdvances in Chromatographyseriesprovides the most up-to-date information on a wide range of developments in chromatographic methods and applications. For more than five decades, scientists and researchers have relied upon this series to cover the state of the art in separation science. With contributions from among the leading researchers around the world, this respected series continues to present timely, cutting-edge reviews in the fields of bio-, analytical, organic, polymer, and pharmaceutical chemistry. With contributions from leading authorities, this is an enriching guide for analytical, organic, inorganic, clinical, and physical chemists; chromatographers; biochemists and biotechnologists; scientists in Academia, government, hospitals and industry in both research and quality control. This is Volume 30 and looks at Selectivity and Retention, originally published in 1989.Table of ContentsMobile and Stationary Phases for Supercritical Fluid Chromatography, Polymer-based Packing Materials for Reversed-Phase Liquid Chromatography, Retention Behavior of Large Polycyclic Armoatic Hydrocarbons in Reversed-Phase Liquid Chromatography, Miniaturisation in High-Performance Liquid Chromatography, Sources of Errors in the Densitometric Evaluation of Thin-Layer Separations with Special regard to Nonlinear problems, Electronic Scanning for the Densitometric Analysis of Flat-bed Separations

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Book SynopsisTheAdvances in Chromatographyseriesprovides the most up-to-date information on a wide range of developments in chromatographic methods and applications. For more than five decades, scientists and researchers have relied upon this series to cover the state of the art in separation science. With contributions from among the leading researchers around the world, this respected series continues to present timely, cutting-edge reviews in the fields of bio-, analytical, organic, polymer, and pharmaceutical chemistry. With contributions from leading authorities, this is an enriching guide for analytical, organic, inorganic, clinical, and physical chemists; chromatographers; biochemists and biotechnologists; scientists in Academia, government, hospitals and industry in both research and quality control. This is Volume 32 originally published in 1991.Table of ContentsPorous Graphitic Carbon in Biomedical Applications, Tryptic Mapping by Reserved Phase Liquid Chromatography, Determination of Dissolved Gases in Water by Gas Chromatography, Separation of Polar Lipid Classes into their Molecular Species Components by Planr and Column Liquid Chromatography, The Use of Chromatography in Forensic Science, HPLC of Explosives Materials.

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Book SynopsisThis work introduces scientists of all disciplines to the chromatographic process and how it functions. The basic principles of chromatographic separation and specific chromatographic procedures, including gas, liquid and thin-layer chromatography, are covered. For each separation method the book details its characteristics, the instrumentation required, the procedures necessary for effective use, areas of application and examples of its use.;This work is intended for analytical chemists, laboratory technicians, and upper-level undergraduate and graduate students in analytical chemistry or separation science courses.Trade Review"Excellent discussions and practical advice are provided for the selection of stationary and mobile phases, as well as the exact type of chromatography needed to accomplish a separation. "---Analytical Chemistry News & Features ". . .a very readable and. . .informative book which is highly recommended for any scientist who needs to use separation techniques as part of their work but is not a separations expert. "---Bioseparation ". . .a[n]. . .instructive presentation of basic intermolecular forces and processes playing a substantial role in understanding the selectivity and resolution of separations, and a nice broad set of practical examples of applications. "---Journal of Chromatography ATable of ContentsThe chromatographic separation; the mechanism of retention; peak dispersion; qualitative analysis; quantitative analysis. Part 1 Gas chromatography: the gas chromatograph; gas chromatography detectors; gas chromatography columns; gas chromatography applications; the liquid chromatograph; liquid chromatography detectors; liquid chromatography columns; liquid chromatography applications. Part 2 Thin layer chromatography: TLC apparatus; thin-layer chromatography techniques.

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Book SynopsisHandbook of Chromatography: Analysis of Lipids provides a valuable review of state-of-the-art applications of chromatographic techniques (TLC, GC, HPLC) and other analytical techniques. Much of this volume is devoted to applications of HPLC (including supercritical fluid chromatography) in the analysis of lipids such as fatty acids, oxygenated fatty acids, enantiomeric acyl- and alkylglycerols, and lipoproteins. The handbook also provides extensive coverage of applications of combinations of various chromatographic techniques used in the analysis of ozonides, anacardic acids, glycerophospholipids, products of lipolysis, artifacts and contaminants in edible fats, acylated proteins, non-caloric lipids, lipophilic vitamins, acyl-Coenzyme A thioesters, dolichols, mycolic acids, technical fats and fat products, and liposomes. Handbook of Chromatography: Analysis of Lipids will be a useful reference for oil chemists, biochemists, fat science technologists, and other scieTable of Contents: Obtaining and Handling Biological Materials and Prefractionating Extracts for Lipid Analysis. Analysis of Lipids by Planar Chromatography. Imaging Plate System for Radio Luminographic Detection of Lipids on Thin-Layer Plates. High Performance Liquid Chromatography as a Tool for the Lipid Chemist and Biochemist. High Performance Liquid Chromatography of Fatty Acids. Analysis of Lipids by On-Line Coupled Liquid Chromatography-Gas Chromatography. Supercritical Fluid Chromatographic Analysis of Lipids. High Performance Liquid Chromatography of Oxygenated Fatty Acids Including Enantiomer Separation. High Performance Liquid Chromatography of Lipoproteins. High Performance Liquid Chromatography of Acylglycerols and Alkylglycerols. Gas Chromatography of Triacylglycerols and Other Lipids in Packed Columns. Gas Chromatography of Fatty Acids and Acylglycerols in Capillary Columns. Radio-Gas Chromatography of Lipids. Ozonides and Ozonolysis of Lipids. Anacardic Acids. Glycerophospholipids. Artifacts, Contaminants and Autoxidation Products of Edible Fats and Oils. Acylated Proteins: Identification of the Attached Fatty Acids and Their Linkages. Non-Caloric Lipids. Lipophilic Vitamins. Acyl-CoA Thioesters. Dolichols and Dolichyl Derivatives. Mycolic Acids. Technical Fats and Oils and Products Derived Therefrom. Analysis of Natural Fats and Oils by Ammonia Negative Ion Tandem Mass Spectrometry. Characterization of Liposomes. Phospholipids from Pulmonary Surfactant and Amniotic Fluid. Large-Scale Chromatography of Lipids. Products of Lipolysis. Index. FEATURES:

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Book SynopsisPreparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compounds. The book contains theoretical background, chemical principles, and relevance of preparative layer chromatography (PLC) to a wide range of applications, particularly in the study of pharmaceuticals and biochemistry. Written by many of the best known and most knowledgeable specialists in the field, the chapters describe all the necessary techniques, current procedures, and superior strategies for selecting the most suitable eluents and designing application-specific PLC systems based on the data being sought. They provide comprehensive instructions, surrounding issues, and suggestions for optimizing optional working techniques withiTrade Review"...A valuable and important supplement to the existing vast chromatographic literature, [this book] demonstrates the potential of planar chromatography for separation and isolation of pure compounds, even from very complex mixtures."-Journal of Liquid Chromatography, August 2006"The contributor[s']...experience integrated in the monograph forms a good basis for the readers."-Instrumentation Science & Technology, August 2006"The [first] eight chapters provide sound theoretical and methodological foundations of PLC. Numerous applications of PLC are reviewed in the second section, based on extensive literature. ...The application reviews provide numerous examples of solving experimental difficulties for the separation of complex mixtures."-Preparative Biochemistry & Biotechnology, August 2006"The book is quite well produced and has a large number of illustrations . . . this volume is a worthy addition to the PLC literature, and a good place to start for the novice." – I.D. Wilson, in Chromatographia, January 2008, Vol. 67, No. 1/2Table of ContentsIntroduction. Adsorption Planar Chromatography in the Non-linear Range: Selected Drawbacks and Selected Guidelines. Sorbents and Precoated Layers in PLC. Selection and Optimization of the Mobile Phase for PLC. Sample Application and Development of Layers. On Methodical Possibilities of the Horizontal Chambers in PLC. Location of Separated Zones by Use of Visualization Reagents, UV Absorbance on Layers Containing a Fluorescent Indicator, and Densitometry. Additional Detection Methods for Separated Zones and Recovery of Substances from the Layer. Medical Applications of PLC. PLC of Hydrophobic Vitamins. PLC of Natural Mixtures. Application of PLC to Lipids. The Use of PLC for Separation of Natural Pigments. Application of PLC to Inorganics and Organometallics. PLC in a Clean-up and Group Fractionaction of Geochemical Samples: A Review of the Commonly Applied Techniques. The Use of PLC for Isolation and Identification of Unknown Compounds from the Frankincense Resin: Strategies for Finding Marker Substances.

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Book SynopsisThin layer chromatography (TLC) is well suited for performing enantioseparations for research as well as larger-scale applications. A fast, inexpensive, and versatile separation technique, there are many practical considerations that contribute to its effectiveness. Thin Layer Chromatography in Chiral Separations and Analysis is the first book to focus solely on the theory, capabilities, and applications of TLC for direct and indirect enantioseparations.The first part of the book examines the fundamental principles of chirality and TLC. It describes the necessary materials, laboratory equipment, procedures, and strategies for the separation, quantification, isolation, and analysis of chiral compounds. The second part evaluates the real-world enantioseparations and densitometric analyses. Emphasizing pharmaceutical applications, the book discusses chiral separation mechanisms and methods for analyzing the chiral purity of diastereoisomers, amino acids, beta-blockers, and NSAIDS. Topics also include commercial stationary phases and chiral modifiers of mobile phases. Thin Layer Chromatography in Chiral Separations and Analysis presents a unified perspective of theory and experimental details underlying the collective developments in the field. The book offers scientists in a variety of disciplines and levels of expertise a complete guide to understanding the current and potential applications of chiral TLC. Table of ContentsIntroduction. Chirality. Commercial Stationary Phases for Enantioseparations. Planar Chromatographic Enantioseparations on Non-commercial CSPs. Planar Chromatographic Enantioseparations on Non-Commercial CCPs. Chiral Modifiers of Mobile Phases. An Overview of the Chiral Separation Mechanisms. Separation of Diastereoisomers by means of TLC. Selected Bottlenecks of Densitometric Detection with Chiral Analytes. Chirality of Pharmaceutical Product Racemates. Chiral Separation of the Selected β-blockers. Chiral Separation of Aminoacid Enantiomers. Chiral Separation of NSAIDs. Determination of Components in Selected Drug Racemates. Chiral Separations using Marfey’s Reagent.

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Book SynopsisCapillary electrochromatography (CEC) is a new and exciting hybrid separation technique that seeks to exploit the combined advantages of both capillary electrophoresis (high efficiencies) and HPLC (mobile and stationary phase selectivity). It is a technique with tremendous potential, especially in the pharmaceutical and biomedical fields. This is the first book to be devoted to the topic and presents reviews by the world leaders in the field on the theory and development of the technique and current and potential future applications. Capillary Electrochromatography provides an excellent introduction to the field for graduates and professionals in industry and academia with an interest in separation science.Trade Review"... a compact and informative review of the principles and practice of this novel and exciting technique ... the book will be very useful to readers new to the field as it is both up-to-date and fully referenced ..." * Chemistry & Industry, Issue 1, 7 January 2002, p 19 *"... an excellent introduction to anyone about to enter the field ... useful and highly informative ..." * Angewandte Chemie, International Edition, Vol 41, No 3, 1 February 2002 *Table of ContentsAn Introduction to Capillary Electrochromatography; The Capillary Electrochromatograph; Supports and Stationary Phases for Capillary Electrochromatography; Electroosmosis in Complex Media: Bulk Transport in CEC; Capillary Electrochromatography with Open Tubular Columns (OTCEC); Capillary Electrochromatography/Mass Spectrometry; Pharmaceutical Applications of Capillary Electrochromatography; Capillary Electrochromatography in Natural Product Research; Subject Index.

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Book SynopsisCyclodextrins can form complexes with a wide variety of organic and inorganic compounds, a property which can prove useful when trying to separate complex mixtures. This book provides an up-to-date and critical evaluation of the application of cyclodextrins in many fields of chromatography (including thin layer, gas-liquid, high performance liquid and supercritical fluid chromatography; capillary electrophoresis; and isotacophoresis). Whilst mainly practical in nature, the book also looks briefly at the theoretical background for the various techniques. Any professional working with chromatography will welcome this unique book as both a practical compilation of methods and a source of reference to the literature regarding the use and impact of cyclodextrins in chromatography.Trade ReviewA good addition to all libraries of pharmaceutical and agrochemical industries and also academic institutions, drug regulatory agencies and environmental agencies. * Biomedical Chromatography, May 2004 (Hassan Y Aboul-Enein) *"... the book is well written and produced, and it can be recommended to anyone interested in an overview of the impact of CD complexes on analytical chromatography ..." * Trends in Analytical Chemistry, Vol 22, No 9, 2003 *"... valuable for anyone working with CDs in chromatographic methods ... the book is well written as a monograph that provides a good review of applications of CDs in chromatographic and electromigration methods. " * Analytical and Bioanalytical Chemistry, 2004, 379 *Table of ContentsPrinciples of Chromatography; Chemistry and Physicochemistry of Cyclodextrins; Use of Cyclodextrins in Gas-Liquid Chromatography; Supercritical Fluid Chromatography; Use of Cyclodextrins in Liquid Chromatography; Use of Cyclodextrins in Electrophoretic Techniques; Subject Index.

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Book SynopsisThis result-oriented primer provides a practical guide to key approaches, methodologies and tools for designing, modelling and simulating chromatographic processes. With an emphasis on real-world applications, this is a must-have for practitioners and researchers working in chemical, biochemical, food and pharmaceutical engineering.Table of Contents1. An approach to modeling chromatographic processes; 2. Linear chromatography: the Russian Lego; 3. Non-linear chromatography: equilibrium theory; 4. Fluid-solid phase equilibria; 5. Mass transfer; 6. Hydrodynamics of chromatographic columns; 7. Simulating chromatographic columns; 8. Counter-current systems; 9. Presentation of chromatographic modes and their optimization; 10. Addressing a few industrial problems; 11. Conclusion.

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Book SynopsisPreparative Chromatography for Separation of Proteins addresses a wide range of the most current modeling techniques, strategies, and case studies of industrial separation of proteins and peptides to aid in the efficiency and efficacy of this broadly-used technique in the purification of biopharmaceuticals.Table of ContentsList of Contributors xv Series Preface xix Preface xxi 1 Model-Based Preparative Chromatography Process Development in the QbD Paradigm 1Arne Staby, Satinder Ahuja, and Anurag S. Rathore 1.1 Motivation 1 1.2 Regulatory Context of Preparative Chromatography and Process Understanding 1 1.3 Application of Mathematical Modeling to Preparative Chromatography 6 Acknowledgements 8 References 8 2 Adsorption Isotherms: Fundamentals and Modeling Aspects 11Jørgen M. Mollerup 2.1 Introduction 11 2.2 Definitions 12 2.3 The Solute Velocity Model 14 2.4 Introduction to the Theory of Equilibrium 17 2.5 Association Equilibria 21 2.6 The Classical Adsorption Isotherm 24 2.7 The Classical Ion Exchange Adsorption Isotherm 26 2.8 Hydrophobic Adsorbents, HIC and RPC 38 2.9 Protein–Protein Association and Adsorption Isotherms 47 2.10 The Adsorption Isotherm of a GLP-1 Analogue 51 2.11 Concluding Remarks 59 Appendix 2.A Classical Thermodynamics 60 References 77 3 Simulation of Process Chromatography 81Bernt Nilsson and Niklas Andersson 3.1 Introduction 81 3.2 Simulation-Based Prediction of Chromatographic Processes 82 3.3 Numerical Methods for Chromatography Simulation 94 3.4 Simulation-Based Model Calibration and Parameter Estimation 96 3.5 Simulation-Based Parametric Analysis of Chromatography 97 3.6 Simulation-Based Optimization of Process Chromatography 101 3.7 Summary 106 Acknowledgement 107 References 108 4 Simplified Methods Based on Mechanistic Models for Understanding and Designing Chromatography Processes for Proteins and Other Biological Products 111Noriko Yoshimoto and Shuichi Yamamoto 4.1 Introduction 111 4.2 HETP and Related Variables in Isocratic Elution 114 4.3 Linear Gradient Elution (LGE) 120 4.4 Applications of the Model 130 4.5 Summary 145 Appendix 4.A Mechanistic Models for Chromatography 149 Appendix 4.B Distribution Coefficient and Binding Sites [20- 149 References 152 5 Development of Continuous Capture Steps in Bioprocess Applications 159Frank Riske and Tom Ransohoff 5.1 Introduction 159 5.2 Economic Rationale for Continuous Processing 160 5.3 Developing a Continuous Capture Step 162 5.4 The Operation of MCC Systems 165 5.5 Modeling MCC Operation 167 5.6 Processing Bioreactor Feeds on a Capture MCC 169 5.7 The Future of MCC 171 References 172 6 Computational Modeling in Bioprocess Development 177Francis Insaidoo, Suvrajit Banerjee, David Roush, and Steven Cramer 6.1 Linkage of Chromatographic Thermodynamics (Affinity, Kinetics, and Capacity) 177 6.2 Binding Maps and Coarse-Grained Modeling 180 6.3 QSPR for Either Classification or Quantification Prediction 188 6.4 All Atoms MD Simulations for Free Solution Studies and Surfaces 192 6.5 Ensemble Average and Comparison of Binding of Different Proteins in Chromatographic Systems 204 6.6 Antibody Homology Modeling and Bioprocess Development 205 6.7 Summary of Gaps and Future State 209 Acknowledgment 212 References 212 7 Chromatographic Scale-Up on a Volume Basis 227Ernst B. Hansen 7.1 Introduction 227 7.2 Theoretical Background 229 7.3 Proof of Concept Examples 232 7.4 Design Applications: How to Scale up from Development Data 233 7.5 Discussion 240 7.6 Recommendations 242 References 245 8 Scaling Up Industrial Protein Chromatography: Where Modeling Can Help 247Chris Antoniou, Justin McCue, Venkatesh Natarajan, Jörg Thömmes, and Qing Sarah Yuan 8.1 Introduction 247 8.2 Packing Quality: Why and How to Ensure Column Packing Quality Across Scales 248 8.3 Process Equipment: Using CFD to Describe Effects of Equipment Design on Column Performance 257 8.4 Long-Term Column Operation at Scale: Impact of Resin Lot-to-Lot Variability 264 8.5 Closing Remarks 265 References 265 9 High-Throughput Process Development 269Silvia M. Pirrung and Marcel Ottens 9.1 Introduction to High-Throughput Process Development in Chromatography 269 9.2 Process Development Approaches 271 9.3 Case Descriptions 279 9.4 Future Directions 286 References 286 10 High-Throughput Column Chromatography Performed on Liquid Handling Stations 293Patrick Diederich and Jürgen Hubbuch 10.1 Introduction 293 10.2 Chromatographic Methods 299 10.3 Results and Discussion 300 10.4 Summary and Conclusion 328 Acknowledgements 329 References 330 11 Lab-Scale Development of Chromatography Processes 333Hong Li, Jennifer Pollard, and Nihal Tugcu 11.1 Introduction 333 11.2 Methodology and Proposed Workflow 336 11.3 Conclusions 377 Acknowledgments 377 References 377 12 Problem Solving by Using Modeling 381Martin P. Breil, Søren S. Frederiksen, Steffen Kidal, and Thomas B. Hansen 12.1 Introduction 381 12.2 Theory 382 12.3 Materials and Methods 385 12.4 Determination of Model Parameters 385 12.5 Optimization In Silico 388 12.6 Extra-Column Effects 390 Abbreviations 397 References 398 13 Modeling Preparative Cation Exchange Chromatography of Monoclonal Antibodies 399Stephen Hunt, Trent Larsen, and Robert J. Todd 13.1 Introduction 399 13.2 Theory 401 13.3 Model Development 403 13.4 Model Application 413 13.5 Conclusions 424 Nomenclature 425 Greek letters 425 References 426 14 Model-Based Process Development in the Biopharmaceutical Industry 429Lars Sejergaard, Haleh Ahmadian, Thomas B. Hansen, Arne Staby, and Ernst B. Hansen 14.1 Introduction 429 14.2 Molecule—FVIII 430 14.3 Overall Process Design 431 14.4 Use of Mathematical Models to Ensure Process Robustness 432 14.5 Experimental Design of Verification Experiments 435 14.6 Discussion 438 14.7 Conclusion 439 Acknowledgements 439 Appendix 14.A Practical MATLAB Guideline to SEC 439 Appendix 14.B Derivation of Models Used for Column Simulations 449 References 455 15 Dynamic Simulations as a Predictive Model for a Multicolumn Chromatography Separation 457Marc Bisschops and Mark Brower 15.1 Introduction 457 15.2 BioSMB Technology 459 15.3 Protein A Model Description 460 15.4 Fitting the Model Parameters 463 15.5 Case Studies 464 15.6 Results for Continuous Chromatography 469 15.7 Conclusions 475 References 476 16 Chemometrics Applications in Process Chromatography 479Anurag S. Rathore and Sumit K. Singh 16.1 Introduction 479 16.2 Data Types 480 16.3 Data Preprocessing 481 16.4 Modeling Approaches 485 16.5 Case Studies of Use of Chemometrics in Process Chromatography 490 16.6 Guidance on Performing MVDA 495 References 497 17 Mid-UV Protein Absorption Spectra and Partial Least Squares Regression as Screening and PAT Tool 501Sigrid Hansen, Nina Brestrich, Arne Staby, and Jürgen Hubbuch 17.1 Introduction 501 17.2 Mid-UV Protein Absorption Spectra and Partial Least Squares Regression 503 17.3 Spectral Similarity and Prediction Precision 511 17.4 Application as a Screening Tool: Analytics for High-Throughput Experiments 516 17.5 Application as a PAT Tool: Selective In-line Quantification and Real-Time Pooling 518 17.6 Case Studies 523 17.7 Conclusion and Outlook 532 References 532 18 Recent Progress Toward More Sustainable Biomanufacturing: Practical Considerations for Use in the Downstream Processing of Protein Products 537Milton T. W. Hearn 18.1 Introduction 537 18.2 The Impact of Individualized Unit Operations versus Integrated Platform Technologies on Sustainable Manufacturing 543 18.3 Implications of Recycling and Reuse in Downstream Processing of Protein Products Generated by Biotechnological Processes: General Considerations 549 18.4 Metrics and Valorization Methods to Assess Process Sustainability 553 18.5 Conclusions and Perspectives 573 Acknowledgment 573 References 574 Index 583

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Book SynopsisPreparative Enantioselective Chromatography Geoffrey B Cox The development of chiral liquid chromatography, facilitating the straightforward separation of enantiomers, was a significant advance in chromatography, leading to widespread application in analytical chemistry.Trade Review"With such a good coverage of the topics and a generous collection of references, the book is a pleasure to read and refer to. The book has struck almost exactly the right note in attractiveness of purpose and method of presentation. The book has a distinctly readable quality. Both the editor and the authors are to be commended for sharing their insights and experience in such an easily accessed volume. The entire volume is lavishly illustrated with tables, graphs and drawings, the design and execution and placement of which are as perfect as the text material they illustrate." Current Engineering Practice 'The book is a delight to read.' 'Cox's book provides chemists and chemical engineers with valuable hints and advises. The authors are, in part, experienced practitioners in the industry and academics at the forefront of this field...at every stage, material is logically presented and easy to access.' 'In summary, this is an excellent compiled book with well-produced text' Gregor Mann, Organic Process Research and Development, September 2005 "This book represents a valuable source of information on the multifaceted challenges one may encounter when performing preparative chromatographic enantiomer seperations, and large-scale chromatography in general ... [this] account will be warmly welcomed by clientele from both industry and academia, as it offers insightful and enjoyable reading, even to the expert." Analytical and Bioanalytical ChemistryTable of ContentsContributors. Preface. 1. Chiral Chromatography in Support of Pharmaceutical Process Research (Christopher J. Welch). 2. Introduction to Preparative Chromatography (Geoffrey B. Cox). 3. Chiral Stationary Phases for Preparative Enantioselective Chromatography (Eric Francotte). 4. Method Development for Preparative Enantioselective Chromatography (Christina Suteu). 5. Scaling-Up of Preparative Chromatographic Enantiomer Separations (Jules Dingenen). 6. Steady-State Recycling and Its use in Chiral Separations (Charles M. Grill and Laurence M. Miller). 7. Simulated Moving Bed and Related Techniques (Luís S. Pais, Vera G. Mata and Alírio E. Rodrigues). 8. Preparative-Scale Supercritical Fluid Chromatography (Manon S. Villeneuve and Luke A. Miller). 9. Equipment for Preparative and Large Size Enantioselective Chromatography (Henri Colin, Olivier Ludemann-Hombourger and Félicie Denet). 10. Case Study in Production-Scale Multicolumn Continuous Chromatography (Michel Hamende). 11. Contract Manufacturing and Outsourcing Considerations (Olivier Dapremont). Appendix: Advanced Concepts. Index.

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Book SynopsisIon-Pair Chromatography (IPC) is a rapidly evolving method for difficult analyses of organic and inorganic ions and ionogenic, neutral, and zwitterionic compounds. The possibilities for this technology continue to grow as novel ion-pair reagents and strategies are introduced at an accelerated level. Compensating for a dearth in the literature, Ion-Pair Chromatography and Related Techniques details the basics and the evolution of this established and easily tunable technique and explains its influence on similar methods.Theoretical modeling to applicationProviding a thorough exploration of the multiplicity of interactions involved in an IPC system, this book emphasizes the progress from theoretical modeling to application. It explores the practical potential of IPC in the life science, medicine, pharmacology, forensic, food, and environmental sectors. It examines the upgrade of column technology and instrumentation to imTrade Review"… provides a broad coverage of the subject of IPC with regard to theoretical features, the parameters influencing the results and the applications. The reader will find a discussion of all aspects of IPC, beginning with the theoretical basis of this technique and its retention mechanism. The author introduces to the reader all the issues connected with stationary phases, ionpair reagents, organic modifiers, the pH of the eluent, temperature, and various detection techniques which may be used in IPC. One can also find much practical advice on how to start with IPC and how to improve results by the use of IPC."—Analytical and Bioanalytical Chemistry, April 2012Table of ContentsIntroduction. Electrolyte Solutions and Historical Concept of Ion-Pairing. Retention Modeling as Function of Mobile Phase Composition. Modeling of Retention as a Function of Analyte Nature. Stationary Phases. Developments in Column Technology and Fast IPC. Ion Pairing Reagents. Organic Modifiers. Role of Eluent pH in IPC. Temperature. Special IPC Modes and Variations. Detection and Combination. Examples of Applications. IPC versus Competitive Techniques. Ion-Pairing in Different Analytical Techniques. Non-Separative Applications of IPC. Conclusions and Future Research Needs.

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Book SynopsisThis book covers the various methods of validation for high performance liquid chromatography - mass spectrometry (LC-MS) analytical techniques and their applications in the analysis of a broad range of drugs in modern clinical pharmacology.Table of ContentsInternal standards for quantitative LC-MS bioanalysis.- Method development, validation and sample analysis for regulated quantitative bioanalysis using LC-MS/MS.- Impact of sampling paper/cards on bioanalytical quantitation via dried blood spots by liquid chromatography - mass spectrometry.- Highly sensitive pharmaceutical and clinical analysis using selective solid-phase extraction coupled to microflow liquid chromatography and isotope-dilution mass spectrometry.- Pitfalls of LC-MS/MS in the clinical laboratory.- Therapeutic drug monitoring to support clinical pharmacogenomics.- Liquid chromatography - mass spectrometry for the determination of antidepressants and some of their major metabolites in human biological matrices.- The analysis of antipsychotic drugs in human biosamples by LC-MS.- Therapeutic drug monitoring of targeted anticancer therapy - tyrosine kinase inhibitors and selective oestrogen receptor modulators: a clinical pharmacology laboratory perspective.- Applications of mass spectrometry in analyses of steroid hormones.- Liquid chromatography - mass spectrometric analysis of tropane alkaloids in mammalian samples: techniques and applications.- Analysis of illicit drugs in human biological samples by LC-MSn.- Use of matrix assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS) in the development of novel small molecule drugs.- A planar integrated micro mass spectrometer.

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Book SynopsisUsed routinely in drug control laboratories, forensic laboratories, and as a research tool, thin layer chromatography (TLC) plays an important role in pharmaceutical drug analyses. It requires less complicated or expensive equipment than other techniques, and has the ability to be performed under field conditions. Filling the need for an up-to-date, complete reference, Thin Layer Chromatography in Drug Analysis covers the most important methods in pharmaceutical applications of TLC, namely, analysis of bulk drug material and pharmaceutical formulations, degradation studies, analysis of biological samples, optimization of the separation of drug classes, and lipophilicity estimation.The book is divided into two parts. Part I is devoted to general topics related to TLC in the context of drug analysis, including the chemical basis of TLC, sample pleparation, the optimization of layers and mobile phases, detection and quantification, analysis of ionic compounds, and separaTable of ContentsPart I Theory of Thin Layer Chromatography in Context of Pharmaceutical Analysis. Overview of Drug Analysis and Structure of the Book. Chemistry of Drugs and Its Influence on Retention. Sorbents and Layers Used in Drug Analysis. Optimization of Mobile Phase Composition. Chromatographic Analysis of Ionic Drugs. Ionic Liquid Additives to Mobile Phases. Chromatographic Analysis of Chiral Drugs. Chambers, Sample Application, and New Devices in the Chromatography of Drugs. 2D Planar Chromatography. Quantitative Detection of Drugs by Densitometry and Video Scanning. Statistical Evaluation and Validation of Quantitative Methods of Drug Analysis. Detection and Identification in TLC Drug Analysis. TLC Determination of Drug Lipophilicity. Screening of Substandard and Fake Drugs in Underdeveloped Countries by TLC. Part II Planar Chromatography of Particular Drug Groups. TLC of Antidepressants and Neuroleptics. TLC of Anxiolytics and Sedatives. TLC of Morphine Analogs. TLC of Nonopioid Analgesics, Anti-Inflammatics, and Antimigraine Drugs. TLC of Ergot Alkaloid Derivatives. Thin-Layer Chromatography of Anesthetics. TLC of Psychostimulants. TLC of Antiepileptics. TLC of Alzheimer’s Disease Medicines. TLC of Antiparkinsonians. Thin-Layer Chromatography of Cardiac Drugs. TLC of Antihypertensive and Antihypotensive Drugs. TLC of Beta-Blockers and Beta-Agonists. TLC of Antithrombotics. TLC of Antihyperlipidemics. TLC of Spasmolytics. TLC of Mucolytic, Antitussive, and Antiasthmatic Drugs. TLC of Neuromuscular Blockers. TLC of Antiulcers. TLC of Antiemetic Drugs. TLC of Steroids and Analogs. TLC of Drugs Used in Obesity and Sexual Dysfunction Treatment. TLC of Prostaglandins. TLC of Diuretics. TLC of Antidiabetics. TLC of Antihistamines. TLC of Vitamins Including Nicotinic Acid Derivatives. TLC of Antiseptics. TLC of Sulfonamides. TLC of Quinolones. Thin-Layer Chromatography of Tuberculostatic Drugs. TLC of Antifungal and Antiprotozoal Drugs. TLC of β-Lactam Antibiotics. TLC of Other Antibiotics. Thin-Layer Chromatography of Antiviral Drugs. Thin-Layer Chromatography of Anticancer Drugs. Uncertainty Factors in the Enantioseparation of Chiral Drugs on Silica Gel Layers. Index.

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Book SynopsisIncreased environmental consciousness within the scientific community has spurred the search for environmentally friendly processes as alternatives to conventional organic solvents. In the past two decades, numerous advancesincluding the use of ionic liquidshave made it possible to develop substitutes for some toxic solvents. Ionic liquids are widely recognized as suitable for use in organic reactions and can also improve the control of product distribution, enhanced reactivity, ease of product recovery, catalyst immobilization, and recycling.Environmentally Friendly Syntheses Using Ionic Liquids presents the latest developments in the field. It also reviews the latest applications in a wide range of fields including biotechnology, nuclear science, medicine, pharmaceuticals, environmental science, and organic and inorganic chemistryall from the standpoint of green sustainable chemistry.Growing interest in the field of ionic liquids will define newer and uTable of ContentsIntroduction. Organic Synthesis Using an Ionic Liquid as a Reaction Medium. Biocatalysis in Ionic Liquids. Deep Eutectic Solvents: Promising Solvents and Nonsolvent Solutions for Biocatalysis. Synthesis and Applications of Ionic Liquids as Pharmaceutical Materials. Ionic Liquids as Versatile Media for Chemical Reactions.

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Book SynopsisAdvanced Separations by Specialized Sorbents opens a new window into sorbent materials, presenting fundamental principles for their syntheses and adsorption properties. The book presents advanced techniques used to create specialized sorbents with a wide range of functions that can be used to enhance the separation and/or purification of useful bioactive compounds, heavy metals, dyes, and other substances. It discusses the most recent developments in the field of separation processes, covering specialized sorbents such as monolith cryogels, composite hydrogels, metal-impregnated ion exchangers, and molecularly imprinted polymers. The book provides a comprehensive discussion of the selectivity in separation processes by composite materials based on synthetic polymers/biopolymers and inorganic particles. It is a comprehensive resource for academic and research scientists as well as students interested in the preparation, characterization, and applicationTable of ContentsComposite Hydrogel Materials. Cryogels for Affinity Chromatography. Particulate/Cell Separations Using Macroporous Monolithic Matrices. Polysaccharide-Based Composite Hydrogels for Removal of Pollutants from Water. Iron Oxide Magnetic Composite Adsorbents for Heavy Metal Pollutant Removal. Biopolymers-Zeolites Composites as Biosorbents for Separation Processes. Metal-Impregnated Ion Exchanger for Selective Removal and Recovery of Trace Phosphate. Molecularly Imprinted Polymers for Water Polishing. Biopolymer-Based Sorbents for Metal Sorption. Mixed-Mode Sorbents in Solid-Phase Extraction. Interpenetrating Polymer Network Composite Hydrogels and Their Applications in Separation Processes. Toward Adaptive Self-Informed Membranes.

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Book SynopsisThis book provides a comprehensive overview of ionic liquid based separation techniques. The glimpse of thermodynamic predictive models along with global optimization techniques will help readers understand the separation techniques at molecular and macroscopic levels. Experimental and characterization techniques are coupled with model based predictions so as to provide multicomponent data for the scientific community. The models will focus more on the a-priori based predictions which gives higher emphasis on hydrogen-bonded systems. Particle Swarm Optimization (PSO) technique will also eventually help the readers to apply optimization technique to an extraction process. The overriding goal of this work is to provide pathways for leading engineers and researchers toward a clear understanding and firm grasp of the phase equilibria of Ionic Liquid systems.Table of Contents1. Introduction2. Liquid-liquid Equilibria: Experiments and Predictions by Gibb’s free energy models3. COSMO-SAC: A predictive model for calculating thermodynamic properties on a-priori basis4. Application of COSMO-SAC in complex phase behavior: Vapor-Liquid-Liquid Equilibria5. Modification in COSMO-SAC6. Particle Swarm Optimization and application to LLE7. Cuckoo Search Optimization and application to LLE

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Book SynopsisGas Chromatography: History, Methods and Applications focuses on the main applications of gas chromatography in clinical and forensic toxicology, mainly in the determination of drugs of abuse including the new psychoactive substances in several types of biological matrices. The authors go on to investigated the analysis of gaseous or volatile substances using sensor gas chromatography equipped with a semiconductor gas sensor detector. The simplicity, ease of handling, and high sensitivity of this method allow results to be obtained rapidly, which may provide valuable information for forensic diagnosis. This compilation addresses the way in which food adulteration practices are potentially harmful to human health and so food safety and authenticity constitute an important issue in food chemistry. The chemical composition of foodstuffs is an excellent indicator of quality, origin, authenticity and/or adulteration. The concluding study aims to determine the organic compounds of vinasse through gas chromatography-mass spectrometry GC-MS. Vinasse is a byproduct of ethanol and poses long-term risk to public health because of its persistent and toxic nature.Table of ContentsPreface; The Role of Gas Chromatography in Clinical and Forensic Toxicology; Application of Sensor Gas Chromatography in Forensic Medicine; Trends of Gas Chromatography-Mass Spectrometry Techniques in Food Authentication; Gas Chromatography-Mass Spectrometry Analysis of Sugarcane Vinasse; Index.

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Book SynopsisFor more than four decades, scientists and researchers have relied on the Advances in Chromatography series for the most up-to-date information on a wide range of developments in chromatographic methods and applications. Volume 44 of this authoritative series once again compiles the work of expert contributors in order to present timely and cutting-edge reviews on a variety of related topics.Each author's clear presentation of topics and vivid illustrations make the material in Advances in Chromatography: Volume 44 accessible and engaging to biochemists and analytical, organic, polymer, and pharmaceutical chemists at all levels of technical skill.Table of ContentsSeparations in Multiple-Channel Microchips. Temperature Effects in Liquid Chromatography. Lipophilicity Measurements by Liquid Chromatography. Concepts and Practice of Multidimensional High-Performance Liquid Chromatography. High-Performance Liquid Chromatography in the Pharmaceutical Industry: Application, Validation, and Regulatory Issues. The Use of Polysaccharide Phases in the Separation of Enantiomers. Chaotropic Effects in RP-HPLC. Chromatography of Difficult and Water-Insoluble Proteins with Organic Solvents.

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Book SynopsisChromatography is the collective term for a set of laboratory techniques for the separation of mixtures. It involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture and allows it to be isolated. Chromatography may be preparative or analytical. The purpose of preparative chromatography is to separate the components of a mixture for further use (and is thus a form of purification). Analytical chromatography is done normally with smaller amounts of material and is for measuring the relative proportions of analytes in a mixture. This new and important book gathers the latest research from around the globe in the study of chomatography and herein highlights such topics as: analysis of veterinary drugs using chromatographic techniques, liquid chromatography for the determination of mycotoxins in foods, chromatography in the research of phenolic secondary metabolites, and others.

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