Description

Book Synopsis
This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.

Table of Contents
Getting Started.

Basics of Separation.

Detection Sensitivity and Selectivity.

Sample Preparation.

The Column.

Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

Gradient Elution.

Systematic Approach to the Reversed-Phase Separation of Regular Samples.

Computer-Assisted Method Development.

Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

Chiral Separations.

Preparative HPLC Separation.

Quantitation (Including Trace Analysis).

Completing the Method: Validation and Transfer.

Appendices.

Index.

Practical HPLC Method Development

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    A Hardback by Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch

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      Book details

      Publisher John Wiley & Sons Inc
      Published 27 March 1997
      ISBN-13 9780471007036
      978-0471007036
      ISBN-10 047100703X
      Also in:
      Chemistry Chromatography

      Description

      Book Synopsis
      This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.

      Table of Contents
      Getting Started.

      Basics of Separation.

      Detection Sensitivity and Selectivity.

      Sample Preparation.

      The Column.

      Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

      Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

      Gradient Elution.

      Systematic Approach to the Reversed-Phase Separation of Regular Samples.

      Computer-Assisted Method Development.

      Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

      Chiral Separations.

      Preparative HPLC Separation.

      Quantitation (Including Trace Analysis).

      Completing the Method: Validation and Transfer.

      Appendices.

      Index.

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