Description

Book Synopsis
This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.

Table of Contents
Getting Started.

Basics of Separation.

Detection Sensitivity and Selectivity.

Sample Preparation.

The Column.

Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

Gradient Elution.

Systematic Approach to the Reversed-Phase Separation of Regular Samples.

Computer-Assisted Method Development.

Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

Chiral Separations.

Preparative HPLC Separation.

Quantitation (Including Trace Analysis).

Completing the Method: Validation and Transfer.

Appendices.

Index.

Practical HPLC Method Development

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A Hardback by Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch

15 in stock


    View other formats and editions of Practical HPLC Method Development by Lloyd R. Snyder

    Publisher: John Wiley & Sons Inc
    Publication Date: 27/03/1997
    ISBN13: 9780471007036, 978-0471007036
    ISBN10: 047100703X
    Also in:
    Chemistry Chromatography

    Description

    Book Synopsis
    This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.

    Table of Contents
    Getting Started.

    Basics of Separation.

    Detection Sensitivity and Selectivity.

    Sample Preparation.

    The Column.

    Non-Ionic Samples: Reversed- and Normal-Phase HPLC.

    Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC.

    Gradient Elution.

    Systematic Approach to the Reversed-Phase Separation of Regular Samples.

    Computer-Assisted Method Development.

    Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.

    Chiral Separations.

    Preparative HPLC Separation.

    Quantitation (Including Trace Analysis).

    Completing the Method: Validation and Transfer.

    Appendices.

    Index.

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