Medical research Books

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

a huge range and FREE tracked UK delivery on ALL orders.

Read more less

Table of ContentsPart I: Overview 1. Making Cardiomyocytes from Pluripotent Stem Cells Peter Karaginius and Yoshinori Yoshida Part II: Generation of Pluripotent Stem Cell-Derived Cardiomyocytes and Cardiac Tissues 2. A method for Large-Scale Cardiac Differentiation, Purification, and Cardiac Spheroid Production of Human Induced Pluripotent Stem Cells Yuika Morita, Shugo Tohyama, Jun Fujita, and Keiichi Fukuda 3. Large-scale Differentiation of Stem Cell-Derived Cardiomyocytes by Stirring-Type Suspension Culture Nagako Sougawa, Shigeru Miyagawa, and Yoshiki Sawa 4. Efficient Method to Dissociate Induced Pluripotent Stem Cell-derived Cardiomyocyte Aggregates into Single Cells Emiko Ito, Shigeru Miyagawa, Yoshinori Yoshida, and Yoshiki Sawa 5. Isolation of Cardiomyocytes Derived from Human Pluripotent Stem Cells using miRNA switches Kenji Miki, Hirohide Saito, and Yoshinori Yoshida 6. Fabrication of Cardiac Constructs using bio-3D Printer Kenichi Arai, Daiki Murata, Shoko Takao, and Koichi Nakayama 7. Fabrication of Thick and Anisotropic Cardiac Tissue on Nanofibrous Substrate for Repairing Infarcted Myocardium Junjun Li, Li Liu, Itsunari Minami, Shigeru Miyagawa, and Yoshiki Sawa 8. Construction of Three-Dimensional Cardiac Tissues Using Layer by Layer Method Maki Takeda, Shigeru Miyagawa, Mitsuru Akashi, and Yoshiki Sawa 9. Generation of Cylindrical Engineered Cardiac Tissues from human iPS Cell-derived Cardiovascular Cell Lineages Hidetoshi Masumoto Part III: Physiological Mesurements using Pluripotent Stem Cell-Derived Cardiomyocytes 10. Protocol for Morphological and Functional Phenotype Analysis of hiPS-derived Cardiomyocytes Jun LI and Jong-Kook LEE 11. Application of FluoVolt Membrane Potential Dye for Induced Pluripotent Stem Cell-derived Cardiac Single Cells and Monolayers Differentiated via Embryoid Bodies Tadashi Takaki and Yoshinori Yoshida 12. Multi-Electrode Array Assays Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes Daisuke Yoshinaga, Yimin Wuriyanghai, and Takeru Makiyama 13. Electrophysiological Analysis of hiPSC-derived Cardiomyocytes Using a Patch-Clamp Technique Yuta Yamamoto, Sayako Hirose, Yimin Wuriyanghai, Daisuke Yoshinaga, and Takeru Makiyama 14. Characterization of Ventricular and Atrial Cardiomyocyte Subtypes from Human Induced Pluripotent Stem Cells Misato Koakutsu, Tadashi Takaki, Kenji Miki, and Yoshinori Yoshida 15. Assessment of Contractility in Human iPS Cell-Derived Cardiomyocytes Using Motion Vector Analysis Yasunari Kanda, Ayano Satsuka, Sayo Hayashi, Mihoko Hagiwara-Nagasawa, and Atsushi Sugiyama 16. Contractile Force Measurement of Engineered Cardiac Tissues Derived from Human iPS Cells Daisuke Sasaki, Katsuhisa Matsuura, and Tatsuya Shimizu 17. A Method for Contraction Force Measurement of iPSC Derived Engineered Cardiac Tissues Yuya Fujiwara, Kohei Deguchi, Kenji Miki, Tomoyuki Nishimoto, and Yoshinori Yoshida Part IV: Transcriptome and Bioinfomatics Analysis 18. Single-cardiomyocyte RNA Sequencing to Dissect the Molecular Pathophysiology of the Heart Manami Kotoh, Seitaro Nomura, Shintaro Yamada,Hiroyuki Aburatani, and Issei Komuro 19. RNA-sequencing Analysis of Differentially Expressed Genes in Human iPSC-derived Cardiomyocytes Chikako Okubo, Megumi Narita, Takuya Yamamoto, and Yoshinori Yoshida 20. Analysis of Transcriptional Profiling of Chamber-specific Human Cardiac Myocytes Derived from Pluripotent Stem Cells Antonio Lucena-Cacace and Yoshinori Yoshida Part V: Gene Editing and CRISPR Technology for Pluripotent Stem Cells 21. Genome Editing in Human Induced Pluripotent Stem Cells (hiPSCs) Shuichiro Higo, Shungo Hikoso, Shigeru Miyagawa, and Yasushi Sakata 22. Generation of Efficient Knock-in Mouse and Human Pluripotent Stem Cells Using CRISPR-Cas9 Tatsuya Anzai, Hiromasa Hara, Chanthra Nawin, Taketaro Sadahiro, Masaki Ieda, Yutaka Hanazono, and Hideki Uosaki 23. CRISPRi/a Screening with Human iPSCs Masataka Nishiga, Lei S. Qi, and Joseph C. Wu Part VI: Transplantation of Pluripotent Stem Cell-Derived Cardiomyocytes 24. Transplantation of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes in a Mouse Myocardial Infarction Model Takeshi Hatani and Yoshinori Yoshida 25. Transplantation of Pluripotent Stem Cell-derived Cardiomyocytes into a Myocardial Infarction Model of Cynomolgus Monkey Hideki Kobayashi, Hajime Ichimura, Noburou Ohashi, and Yuji Shiba

Read more less

Book SynopsisClelia Falletti is Associate Professor at Sapienza University of Rome, Italy. Since 1981 she has participated in research by ISTA (International School of Theatre Anthropology) directed by Eugenio Barba; she is dramaturg for Teatro Potlach, and co-editor of theatre books series with two Italian publishing houses.Gabriele Sofia teaches theatre studies and physical theatre at Paul Valéry University, Montpellier, France. Since 2006 he has carried out an interdisciplinary research project on the neurophysiology of the actor and the spectator between the Sapienza University of Rome and the Maisons des Sciences de l'Homme Paris Nord. From 2009 to 2013 he promoted and organized five editions of the International Conference Dialogues between Theatre and Neuroscience at Sapienza.Victor Jacono, PhD in Performance Studies, teaches at the MCAST Institute for the Creative Arts and Drama at the Alternative Learning Program, Malta.Trade ReviewA key strength of the book lies in the diversity of its perspectives ... The book makes a significant contribution to this field of study, particularly since there is arguably a lacuna when it comes to the featuring of theatre ... in scientific publications ... Its speciality is its multifaceted perspective. * South African Theatre Journal *The essays provide a range of information, applications, and insights from the intersections of science and performance. * Theatre Journal *Table of ContentsPreface Part One: Theatre as a space of relationships: a neurocognitive approach 1. Editorial Introduction: The space of shared action (Clelia Falletti, Sapienza University of Rome, Italy) 2. “Mirror mechanism” and motor behavior (Maria Alessandra Umiltà, neuroscientist, University of Parma, Italy) 3. Body presence and extra-personal space perception (Giorgia Committeri and Chiara Fini neuroscientists, University of Chieti, Italy) 4. The actor at the circus. Towards a cognitive approach (Philippe Goudard, University Paul Valéry Montpellier 3, France) Part Two: The spectator’s performative experience and “Embodied theatrology” 5. Editorial introduction: Towards an “Embodied Theatrology”? (Gabriele Sofia, University Paul Valéry Montpellier 3, France) 6. Body and corporeality. A small multidisciplinary glossary (Marco De Marinis, University of Bologna, Italy) 7. Audiences’ experience of proximity and co-presence in live dance performance (Corinne Jola, neuroscientist and choreographer, Abertay University, Dundee and Matthew Reason, Faculty of Arts, York St John University, UK) 8. “Theatre and science”. Some reflections on the theatre effectiveness mechanism in Antonin Artaud (Lorraine Dumenil, University Paris 3 Sorbonne Nouvelle, France) Part Three: The complexity of theatre and human cognition 9. Editorial Introduction: The Complexity of the actor’s pedagogy and human cognition (Victor Jacono, Sapienza University of Rome, Italy) 10. A rope over an abyss (John J. Schranz, University of Malta) 11. The actor’s embodied language. Preliminary indications from a pilot experiment (Gabriele Sofia; Silvia Spadacenta, neuroscientist, University of Tu¨bingen, Germany; Clelia Falletti; Giovanni Mirabella, neuroscientist, Sapienza University of Rome, Italy) 12. Perception and the organization of time in the theatre (Luciano Mariti, Sapienza University of Rome, Italy) Part Four: Interdisciplinary perspectives in applied performance 13. Editorial introduction: Does art therapy work as a rehabilitative tool? (Giovanni Mirabella neuroscientist, Sapienza University of Rome, Italy) 14. Use of theatrical techniques and elements as interventions for autism spectrum disorders (Jenna Gabriel, Harvard University, USA; Elisa Angevin, Columbia University, USA; Tamara Rosen and Matthew D. Lerner, Stony Brook University, USA) 15. Theatre is a valuable tool for parkinson’s disease rehabilitation (Nicola Modugno, neurologist, IRCCS, Neuromed, Pozzilli; Imogen Kusch, theatre director of the Klesidra Company, Rome and Giovanni Mirabella) 16. Theatre and therapy: care, cure or illusion? (Jean-Marie Pradier, Ethnoscenologist, University Paris 8 – MSH-Paris Nord, France) Afterword Endnotes Index

Read more less

Book SynopsisThousands of methods have been developed in the various biomedical disciplines, and those covered in this book represent the basic, essential and most widely used methods in several different disciplines. Table of Contents1: Detection And Analysis Of Proteins A. Introduction B. Basic methods for protein analysis C. Characterization of primary, secondary, tertiary and quaternary structures of proteins 2: Detection And Analysis Of Nucleic Acids A. Introduction B. Basic methods for nucleic acid analysis C. DNA sequencing D. Detection and quantitation of RNA 3: Recombinant DNA Techniques: Cloning And Manipulation Of DNA A. Introduction B. Plasmid and viral vectors C. Libraries D. Site Directed Mutagenesis E. Polymerase chain reaction (PCR) F. Screening for differentially expressed genes (DEGs) G. Promoter-protein interactions H. Silencing gene expression I. Forensics and DNA technology 4: Antibody-Based Techniques A. Introduction B. Monoclonal antibodies C. Purification and use of antibodies D. Flow cytometry and fluorescence activated cell sorting (FACS) E. Other assays using antibodies 5: Microscopy: Imaging Of Biological Specimens A. Introduction B. Light microscopy C. Fluorescence and laser confocal microarray microscopy D. Electron Microscopy (EM) E. Magnetic resonance imaging (MRI) 6: The Derivation And Manipulation Of Experimental Animals In Biomedical Sciences A. Introduction B. Inbred strains of mice C. Congenic strains of mice D. Transgenic mice E. Targeted transgenes: 'knockout' and 'knockin' mice F. Other uses of mice in biomedical research References Some excellent methodology manuals Index

Read more less

Book SynopsisPrinciples of Biophysical Inquiry.- Introduction: To the Student First Edition.- Philosophy and Practice of Biophysical Study.- Overview of the Biological System Under Study.- Physical Thoughts, Biological Systems The Application of Modeling Principles to Understanding Biological Systems.- Probability and Statistics.- Foundations.- Energy and Force The Prime Observables.- Biophysical Forces in Molecular Systems.- Physical Principles: Quantum Mechanics.- Chemical Principles.- Measuring the Energy of a System: Energetics and the First Law of Thermodynamics.- Entropy and the Second Law of Thermodynamics.- Which Way Is That System Going? The Gibbs Free Energy.- The Thermodynamics of Phase Equilibria.- Building a Model of Biomolecular Structure.- Water: A Unique Solvent and Vital Component of Life.- IonSolvent Interactions.- IonIon Interactions.- Lipids in Aqueous Solution.- Macromolecules in Solution.- Molecular Modeling Mapping Biochemical State Space.- The Electrified Interphase.- Function and Action Biological State Space.- Transport A Non-equilibrium Process.- Flow in a Chemical Potential Field: Diffusion.- Flow in an Electric Field: Conduction.- Forces Across Membranes.- Kinetics Chemical Kinetics.- Dynamic Bioelectrochemistry Charge Transfer in Biological Systems.- Methods for the Measuring Structure and Function.- Separation and Characterization of Biomolecules Based on Macroscopic Properties.- Analysis of Molecular Structure with Electronic Spectroscopy.- Molecular Structure from Scattering Phenomena.- Analysis of Structure Microscopy.- Epilogue.- Physical Constants.Table of ContentsPREFACE PART I: Principles of Biophysical Inquiry Chapter 1 Introduction: “To the Student” Chapter 2 Philosophy and Practice of Biophysical Study Chapter 3 Overview of the Biological System Under Study – Descriptive Models Chapter 4 Physical Thoughts, Biological Systems - The application of modeling principles to understanding biological systems Chapter 5 Probability and Statistics PART II: Foundations Chapter 6 Physical Principles: Energy - The Prime Observable Chapter 7 Biophysical Forces in Molecular Systems Chapter 8 An Introduction to Quantum Mechanics Chapter 9 Chemical Principles Chapter 10 Measuring the Energy of a System: Energetics and the First Law of Thermodynamics Chapter 11 Entropy and the Second Law of Thermodynamics Chapter 12 Which Way Did That System Go? The Gibbs Free Energy Chapter 13 The Thermodynamics of Phase Equilibria PART III: Building a Model of Biomolecular Structure Chapter 14 Water: A Unique Structure, A Unique Solvent Chapter 15 Ion-Solvent Interactions Chapter 16 Ion-Ion Interactions Chapter 17 Lipids in Aqueous Solution Chapter 18 Macromolecules in Solution Chapter 19 Molecular Modeling - Mapping Biochemical State Space Chapter 20 The Electrified Interphase PART IV: Function and Action Biological State Space Chapter 21 Transport and Kinetics: Processes Not at Equilibrium Chapter 22 Flow in a Chemical Potential Field: Diffusion Chapter 23 Flow in an Electrical Field: Conduction Chapter 24 Forces Across Membranes Chapter 25 Kinetics - Chemical Kinetics Chapter 26 Bioelectrochemistry – Charge Transfer in Biological Systems PART V: Methods for the Measuring Structure and Function Chapter 27 Separation and Characterization of Biomolecules Based on Macroscopic Properties (with Kristin E. Bergethon) Chapter 28 Determining Structure by molecular interactions with photons: Electronic Spectroscopy (with Kristin Bergethon) Chapter 29 Determining Structure by molecular interactions with photons: ScatteringPhenomena Chapter 30 Analysis of Structure – Microscopy Chapter 31 Epilogue Chapter 32 Physical Constants PART VI: APPENDICES Appendix A Review of Mathematical Methods Appendix B Quantum Electrodynamics Appendix C The Pre-Socratic Roots of Modern Science Appendix D The Poisson Function Appendix E Assumptions of a Kinetic Theory of Ideal Gas Behavior Appendix F Determination of a Field from the Potential Appendix G Geometric Optics Appendix H The Compton Effect Appendix I Hamilton's Principle of Least Action/Fermat's Principle of Least Time Appendix J Energy of Interaction between ions Appendix K Derivation of the Statement, Qrev > Qirrev Appendix L Derivation of the Clausius-Clapeyron Equation Appendix M Derivation of the van't Hoff Equation for Osmotic Pressure Appendix N Pseudoforces Appendix O Work of charging and discharging a rigid sphere Appendix P Review of Electrical Circuits Appendix Q Fermi's Golden Rule Appendix R Adiabatic vs non-Adiabatic processes

Read more less

Book Synopsis1 / Organism Theory: The Dictates of Genes, the Meanings of Environment.- Primary Rules of Reproductive Fitness.- Grand Strategies of Maintenance and Spread.- Rules of Conduct Dictated by the Mechanisms of Gene Transmission and Expression.- The Environment.- 2 / CognitionPredictability.- A Predictable Environment.- Relation Between Pattern Matching and the Reafference Principle.- Pattern Matching.- Theory of Emotionality.- Intelligence, Creativity, Memory.- Structuring and Ontogeny of Cognitive Patterns.- Prediction With the Theory of Emotionality and Pattern Matching: From Sheep in Pastures to Conventional Wisdom.- 3 / Communication.- Origin, Components, and Definitions.- Some Principles of Communication: The Law of Least Effort.- Some Rules Governing Semantic and Pragmatic Structure of Signals: Novelty.- Antithesis or the Law of the Opposite.- Stimulus Contrast.- Redundancy.- Intention MovementsIconic Signals.- Levels of Communication.- 4 / Aggression.- Competition.- Agonistic AdaptaTable of Contents1 / Organism Theory: The Dictates of Genes, the Meanings of Environment.- Primary Rules of Reproductive Fitness.- Grand Strategies of Maintenance and Spread.- Rules of Conduct Dictated by the Mechanisms of Gene Transmission and Expression.- The Environment.- 2 / Cognition—Predictability.- A Predictable Environment.- Relation Between Pattern Matching and the Reafference Principle.- Pattern Matching.- Theory of Emotionality.- Intelligence, Creativity, Memory.- Structuring and Ontogeny of Cognitive Patterns.- Prediction With the Theory of Emotionality and Pattern Matching: From Sheep in Pastures to Conventional Wisdom.- 3 / Communication.- Origin, Components, and Definitions.- Some Principles of Communication: The Law of Least Effort.- Some Rules Governing Semantic and Pragmatic Structure of Signals: Novelty.- Antithesis or the Law of the Opposite.- Stimulus Contrast.- Redundancy.- Intention Movements—Iconic Signals.- Levels of Communication.- 4 / Aggression.- Competition.- Agonistic Adaptations.- Definition of Aggression.- The Phylogeny Fallacy.- Misadventures in Aggression.- Weapons and Defenses.- Retaliation as a Control on the Evolution of Aggression.- Armament and Ecological Strategy.- Circumstances and Aggression: Some Rules.- 5 / Dominance Displays: The Biology of Art, Pride—and Materialism.- Dominance Displays: Their Raison d’ Etre.- Characteristics of Dominance Displays.- Attention-Guiding Organs: The Biology of Art.- Quantitative Rules in Dominance Displays.- Ontogeny of Dominance Displays.- Evolutionary Directions in Dominance Displays.- Human Dominance Displays: Biological Basis of Displays and Their Cultural Enhancement.- Humor, Laughter, Courtesy.- Cultural Dominance Displays and Their Rules.- 6 / How Genes Communicate With the Environment—The Biology of Inequality.- Phenotype Syndromes in Vertebrates.- Social and Psychological Factors Affecting Population Quality.- Two Explanatory Hypotheses Based on Cortical Dominance, Growth Priority, and Blood Flow.- Evolution and Adaptive Significance of Growth Priorities in Tissues: The Dispersal and Maintenance Phenotypes.- Phenotype Plasticity and Man.- 7 / Mammalian Systems.- Prelude to Mammalhood.- A New Ecological Profession or Ecological Niche.- From Reptile to Mammal: Locomotion, Tonus, Heat Production and Control.- From Bolting to Chewing.- Alterations of Digestive Glands and Ureotelism.- Bigger Kidney and Control Glands.- Larger Lungs, Bigger Heart, Simpler Circulation.- Adjusting Surface-to-Mass Ratio.- A Consequence of Tail Reduction: New Weapons and Defenses.- Toward Homeothermy.- Evolution of Hair.- A New Breathing System.- More Erythrocytes.- Olfaction, Scent Glands, Touch, and Hearing.- “Mnemotaxis”.- The Nest for the Young and for Digestion, Energy Stores, and Digging Paws.- Evolution of Parental Care and Lactation.- Diphyodonty.- New Bone Microstructure.- Sleep and Its Early Evolution.- Mammalian Liabilities: High-Cost, Short Life, High Aggression, Poor Performance in Heat and Desert.- 8 / Life Forms and Extinction.- Dynasties of the Past.- Extinction.- Life Forms.- 9 / The Ice Ages.- Tertiary and Pleistocene Giants and the Dispersal Theory.- The Tundra Ecosystem.- The Periglacial Ecosystem: The St. Elias Range.- The Elements of the Periglacial Ecosystem.- Habitat Mosaics as a Function of Periglacial Climatic and Edaphic Factors.- Biomass of Large Mammals.- Large Mammal Habitats in the Periglacial Zone.- Climatic Factors and Megafaunal Extinctions.- 10 / Prehumans.- The Early Hominids.- Adaptations to the Wet Savannah.- Adaptive Syndrome of the Gracile Hominids.- Meat Diet: For and Against.- Social System.- Territoriality.- Significance of Tools.- Evolution of Conscience, Imitation and Faith.- 11 / The Homo erectus Stage.- Adaptive Successions: From Savannah to Steppe.- Australopithecine versus early Homo Characteristics.- Characteristics of the Steppe.- From Opportunistic to Systematic Hunting.- Roots of Language.- Geographic Dispersal and the Evolution of Homo erectus: Applying the Dispersal Theory.- An Overview of Homo erectus.- 12 / The First Advance to the Glaciers.- Late Acheulean Man and Temperate Climates.- Cooperative Hunting.- Evolution of Hairlessness.- Dictates of Cold Environments.- Neanderthal Man’s Characteristics.- On the Adaptive Syndrome of Neanderthal Man.- The Extinction of Neanderthal Man.- 13 / On the Evolution of Modern Man.- Early Environmental Settings.- Meat Conservation and its Consequences.- Chronologie Time and Big-Game Migrations.- Distance Confrontation Hunting and Complex Cooperative Hunting.- Before Cro-Magnon.- Some Attributes of Cro-Magnon.- The Early Anxieties.- Megafauna and Late Paleolithic Hunters.- On the Ecology of Bonding Male and Female.- Effects of Monogamy on the Dominance Displays and Courtship of Homo.- The Social Feedback System.- Prerequisites of Cooperation: Music, Dancing, Laughter, Language, Games.- Music: Its Functions and Possible Origins.- Extended Family.- Relict Behaviors.- Sexual Displays.- Neoteny.- 14 / From Periglacial to Artificial Environments.- Hunting Economics.- The Vitamin C Problem.- The Vitamin D Problem.- Parasites, Diseases, and Periglacial Hygiene.- How to Maximize Intellectual Development, Speed of Pattern Matching, and Creativity.- Magic Numbers.- The Social Environment.- The Mesolithic.- Geographic Dispersal of Modern Man..- On the Origin of Medical and Pharmaceutical Knowledge.- The Beginning of Artificial Environments and Some of Their Biological Effects.- 15 / Health, Professionals, and Creature Comforts.- Professionals and Intervention.- On Health and a Healthful Environment.- Evolutionary Model of a Healthful Environment: Its Rationale.- Normative Environmental Criteria.- Quality of Life.- Creature Comforts.- Testing the Evolutionary Model.- The Evolutionary Model in Decision Making: Examples.- What are the Costs of Maximizing Individual Development?.- References.

Read more less

Book SynopsisAvian Influenza Virus, Second Edition aims to provide the essential methods used in working with animal influenza viruses, and to compile more advanced information that will guide the user in designing influenza studies.Trade ReviewFrom the book reviews:“This methodology book presents laboratory protocols for working with animal influenza viruses. … Because it covers a broad spectrum of research, this book is best suited for new students or technicians researching animal influenza viruses or for experienced researchers in one area of animal influenza viruses wishing to work with influenza viruses from other species. … Most sections contain excellent illustrations, photographs, and tables that help readers understand the assays, something that is often lacking in protocol books.” (Elizabeth A. Driskell, Doody’s Book Reviews, September, 2014)Table of ContentsPart I. General 1. Hemagglutination Assay for Influenza Virus Mary Lea Killian 2. Hemagglutination-Inhibition Assay for Influenza Virus Subtype Identification and the Detection and Quantitation of Serum Antibodies to Influenza Virus Janice. C. Pedersen 3. Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Virus Subtype or Neuraminidase Antibody Specificity Janice. C. Pedersen 4. Reverse Genetics of Influenza Virus Chang-Won Lee 5. Immunohistochemical Staining of Influenza Virus in Tissues Mary J. Pantin-Jackwood Part II. Avian Influenza 6. A Brief Introduction to Avian Influenza Virus Erica Spackman 7. Wild Bird Surveillance for Avian Influenza Virus Justin D. Brown, Rebecca Poulson, and David E. Stallknecht 8. Avian Influenza Virus Sample Types, Collection and Handling Mary Lea Killian 9. Avian Influenza Virus RNA Extraction Erica Spackman and Scott A. Lee 10. Avian Influenza Virus Detection and Quantitation by Real-Time RT-PCR Erica Spackman 11. Influenza Subtype Identification with Molecular Methods Erica Spackman 12. Avian Influenza Virus Isolation, Propagation and Titration in Embryonated Chicken Eggs Erica Spackman and Mary Lea Killian 13. Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus Terra A. Jenson 14. Detection of Influenza A Antibodies in Avian Serum Samples by ELISA Len Chappell, Mary Lea Killian, and Erica Spackman 15. Conducting Influenza Virus Pathogenesis Studies in Avian Species Carol Cardona, David A. Halvorson, Justin Brown, Jeffrey Hall, and Mary Pantin-Jackwood 16. Laboratory Methods for Assessing and Licensing Influenza Vaccines for Poultry David E. Swayne 17. Detection of Cell Mediated Immune Response to Avian Influenza Viruses Darrell R. Kapczynski 18. Characterization of Cytokine Expression Induced by Avian Influenza Virus Infection with Real-Time RT-PCR Darrell R. Kapczynski, Hai Jun Jiang, and Michael H. Kogut 19. Newcastle Disease Virus Detection and Differentiation from Avian Influenza Patti J. Miller and Mia Kim Torchetti 20. A Brief Introduction to Influenza A Virus in Swine Amy L. Vincent, Kelly M. Lager, and Tavis K. Anderson 21. Sample Types, Collection, and Transport for Influenza A Viruses of Swine Marie Culhane and Susan Detmer 22. Isolation of Swine Influenza Virus in Cell Cultures and Embryonated Chicken Eggs Jianqiang Zhang and Phillip C. Gauger 23. RNA Extraction from Swine Samples and Detection of Influenza A Virus in Swine by Real-Time RT-PCR Jianqiang Zhang and Karen M Harmon 24. Hemagglutinin Inhibition Assay with Swine Sera Pravina Kitikoon, Phillip C. Gauger, and Amy L. Vincent 25. Enzyme-Linked Immunosorbent Assay for Detection of Serum or Mucosal Isotype Specific IgG and IgA Whole Virus Antibody to Influenza A Virus in Swine Phillip C. Gauger, Crystal L. Loving, and Amy L. Vincent 26. Serum Virus Neutralization Assay for Detection and Quantitation of Serum Neutralizing Antibodies to Influenza A Virus in Swine Phillip C. Gauger and Amy L. Vincent 27. Microneutralization Assay for Swine Influenza Virus in Swine Serum Pravina Kitikoon and Amy L. Vincent 28. Analyzing Swine Sera for Functional Antibody Titers Against Influenza A Neuraminidase Proteins Using an Enzyme-Linked Lectin Assay (ELLA) Matthew R. Sandbulte and Maryna C. Eichelberger 29. Antibody Secreting Cell Assay for Influenza A Virus in Swine Pravina Kitikoon, Crystal L. Loving, and Amy L. Vincent 30. In vivo Models for Pathotyping and Vaccine Efficacy for Swine Influenza Kelly M. Lager and Amy L. Vincent Part III. Equine Influenza 31. A Brief Introduction to Equine Influenza and Equine Influenza Viruses Thomas M. Chambers 32. Equine Influenza Diagnosis: Sample Collection and Transport Thomas M. Chambers and Stephanie E. Reedy 33. RNA Extraction from Equine Samples for Equine Influenza Virus Udeni B. R. Balasuriya 34. Type A Influenza Virus Detection from Horses by Real-Time RT-PCR and Insulated Isothermal RT-PCR Udeni B. R. Balasuriya 35. Equine Influenza Culture Methods Thomas M. Chambers and Stephanie E. Reedy 36. Equine Influenza Serological Methods Thomas M. Chambers and Stephanie E. Reedy

Read more less

Book SynopsisThe aim of this volume is to make computer programs for analyzing human genetic data more easily accessible to the beginner.Statistical Human Genetics: Methods and Protocols, Second Edition provides updated and new chapters detailing genetic terms, analysis software, and how to interpret the program outputs.Table of Contents1. Statistical Genetic TerminologyRobert Elston, Jaya Satagopan, and Shuying Sun 2. Identification of Genotype ErrorsJeffery O’Connell and Yin Yao 3. Detecting Pedigree Relationship ErrorsLei Sun 4. Identifying Cryptic RelationshipsLei Sun, Apostolos Dimitromanolakis, and Wei-Min Chen 5. Estimating Allele FrequenciesIndra Adrianto and Courtney Montgomery 6. Testing Departure from Hardy-Weinberg ProportionsJian Wang and Sanjay Shete​ 7. Estimating Disequilibrium CoefficientsMaren Vens and Andreas Ziegler 8. Detecting Familial AggregationAdam C. Naj and Terri H. Beaty 9. Estimating Heritability from Twin StudiesKatrina Grasby, Karin J.H. Verweij, Miriam A. Mosing, Brendan P. Zietsch, and Sarah E. Medland 10. Estimating Heritability from Nuclear Family and Pedigree DataMurielle Bochud 11. Correcting for AscertainmentWarren Ewens and Robert C. Elston 12. Segregation Analysis using the Unified ModelXiangqing Sun 13. Design Considerations for Genetic Linkage and Association StudiesJérémie Nsengimana and D Timothy Bishop 14. Model-based Linkage Analysis of a Quantitative Trait Yeunjoo E. Song, Sunah Song, and Audrey H. Schnell 15. Model-based Linkage Analysis of a Binary TraitRita M. Cantor 16. Model-free Linkage Analysis of a Quantitative TraitNathan J. Morris and Catherine M. Stein 17. Model-free Linkage Analysis of a Binary TraitWei Xu, Jin Ma, Celia M.T. Greenwood, Andrew D. Paterson, and Shelley B. Bull 18. Single Marker Association Analysis for Unrelated SamplesGang Zheng­, Ao Yuan, Qizhai Li, and Joseph L. Gastwirth 19. Single Marker Family-based Association Analysis Conditional on Parental InformationRen-Hua Chung, Daniel D. Kinnamon, and Eden R. Martin 20. Single Marker Family-based Association Analysis not Conditional on Parental InformationJunghyun Namkung and Sungho Won 21. Calibrating Population Stratification in Association AnalysisHuaizhen Qin and Xiaofeng Zhu 22. Cross-phenotype Association Analysis using Summary Statistics from GWASXiaoyin Li and Xiaofeng Zhu 23. Haplotype Inference Sunah Song, Xin Li, and Jing Li 24. Multi-SNP Haplotype Analysis Methods for Association AnalysisDaniel O. Stram 25. The Analysis of Ethnic MixturesXiaofeng Zhu and Heming Wang 26. Detecting Multiethnic Rare VariantsWeiwei Ouyang, Xiaofeng Zhu, and Huaizhen Qin 27. Identifying Gene Interaction NetworksDanica Wiredja and Gurkan Bebek 28. Structural Equation ModelingCatherine M. Stein, Nathan J. Morris, Noémi B. Hall, and Nora L. Nock 29. Mendelian RandomizationSandeep Grover, Fabiola Del Greco M, Catherine M. Stein, and Andreas Ziegler 30. Preprocessing and Quality Control for Whole Genome Sequences from the Illumina HiSeq X PlatformMarvin N. Wright 31. Statistical Approaches for Processing and Analyzing Microbiome DataXuan Zhu, Jian Wang, Cielito Reyes-Gibby, and Sanjay Shete

Read more less

Book Synopsis

Read more less