Cellular biology (cytology) Books
John Libbey & Co Liposomes in Cell Biology and Pharmacology
Book Synopsis
£37.39
Institute for the Study of Peak States Press Subcellular Psychobiology Diagnosis Handbook Subcellular Causes of Psychological Symptoms 1 Peak States Therapy
£21.60
Taylor & Francis Ltd Atlas of Terrestrial Mammal Limbs
Book SynopsisAtlas of Terrestrial Mammal Limbs is the first comprehensive and detailed anatomy book on a broad phylogenetic and ecological range of mammals. This extraordinary new work features more than 400 photographs and illustrations visualizing the limb musculature of 28 different species. Standardized views of the dissected bodies and concise text descriptions make it easy to compare the anatomy across different taxa. It provides tables of nomenclature and comparative muscle maps (schematic drawings on the origins and insertions of the muscles onto bones) in a diversity of animals. Atlas of Terrestrial Mammal Limbs is a reliable reference and an indispensable volume for all students and professional researchers in biology, paleontology, and veterinary medicine.Key Features: Provides an overview of the anatomy of the mammalian limb Includes osteological correlates of the limb muscles <Table of Contents Introduction Part I Chapter 1 Limbs and Locomotion Chapter 2 Comparative Anatomy 2.1 Anatomical Terms of Location 2.2 Limb skeleton 2.3 Limb musculature 2.4 Muscle maps Part II Chapter 1 Martes foina (European stone marten) Chapter 2 Meles meles (European badger) Chapter 3 Nasua nasua (South American coati) Chapter 4 Potos flavus (Kinkajou) Chapter 5 Cuon alpinus (Dhole) Chapter 6 Vulpes vulpes (Red fox) Chapter 7 Herpestes auropunctatus (Small Indian mongoose) Chapter 8 Cryptoprocta ferox (Fossa) Chapter 9 Hyaena hyaena (Striped hyena) Chapter 10 Acinonyx jubatus (Cheetah) Chapter 11 Panthera leo (Lion) Chapter 12 Manis tricuspis (Tree pangolin) Chapter 13 Sus scrofa (Wild boar) Chapter 14 Capreolus capreolus (European roe deer) Chapter 15 Erinaceus europaeus (European hedgehog) Chapter 16 Chiropotes satanas (Brown-bearded saki) Chapter 17 Cuniculus oryctolagus (European rabbit) Chapter 18 Laonastes aenigmamus (Laotian rock rat or kha-nyou) Chapter 19 Octodon degus (Degu) Chapter 20 Sciurus vulgaris (Eurasion red squirrel) Chapter 21 Pedetes capensis (Springhare) Chapter 22 Mesocricetus auratus (Golden hamster) Chapter 23 Meriones unguiculatus (Mongolian gerbil) Chapter 24 Rattus norvegicus (Norway rat) Chapter 25 Elephantulus brachyrhynchus (Short-snouted elephant shrew) Chapter 26 Bradypus tridactylus (Pale-Throated Three-Toed sloth) Chapter 27 Dasyurus viverrinus (Easter quoll) Chapter 28 Philander opoussum (Gray four-eyed opossum) Part III Muscle synonyms Non-exhaustive list of references on mammalian limb muscle anatomy Index
£43.69
Taylor & Francis Ltd Landmark Experiments in Protein Science
Book SynopsisProteins are the workhorses of cells, performing most of the important functions which allow cells to use nutrients and grow, communicate among each other, and importantly, die if aberrant behavior is detected. How were proteins discovered? What is their role in cells? How do dysfunctional proteins give rise to cancers? Landmark Experiments in Protein Science explores the manner in which the inner workings of cells were elucidated, with a special emphasis on the role of proteins. Experiments are discussed in a manner as to understand what questions were being asked that prompted the experiments and what technical challenges were faced in the process; and results are presented and discussed using primary data and graphs.Key Features Describes landmark experiments in cell biology and biochemistry. Discusses the How and Why of historically important experiments. Includes primary, original data and gTable of ContentsChapter 1: Prelude to Biology: A History of Chemistry Chapter 2: The Cell and Heredity Chapter 3: Discovery of Proteins and Enzymes Chapter 4: Protein and DNA Subunits Chapter 5: The Energy of Cells: Glycolysis and the Krebs Cycle Chapter 6: Protein and DNA Structure Chapter 7: Protein Synthesis Part I: Localization of Protein Translation Chapter 8: Protein Synthesis Part II: The Mechanism of Protein Translation Chapter 9: The Energy of Cells: Oxidative Phosphorylation Chapter 10: The Energy of Cells: The Mechanism of ATP Synthesis Chapter 11: Techniques Chapter 12: Cell Signaling Part I: The role of Phosphorylation Chapter 13: Cell Signaling Part II: G-protein-coupled Receptors Chapter 14: The Secretory Pathway Chapter 15: The Mechanism of Cell Death Chapter 16: The Biology of Cancer Index
£117.00
Taylor & Francis Ltd Landmark Experiments in Protein Science
Book SynopsisProteins are the workhorses of cells, performing most of the important functions which allow cells to use nutrients and grow, communicate among each other, and importantly, die if aberrant behavior is detected. How were proteins discovered? What is their role in cells? How do dysfunctional proteins give rise to cancers? Landmark Experiments in Protein Science explores the manner in which the inner workings of cells were elucidated, with a special emphasis on the role of proteins. Experiments are discussed in a manner as to understand what questions were being asked that prompted the experiments and what technical challenges were faced in the process; and results are presented and discussed using primary data and graphs.Key Features Describes landmark experiments in cell biology and biochemistry. Discusses the How and Why of historically important experiments. Includes primary, original data and gTable of ContentsChapter 1: Prelude to Biology: A History of Chemistry Chapter 2: The Cell and Heredity Chapter 3: Discovery of Proteins and Enzymes Chapter 4: Protein and DNA Subunits Chapter 5: The Energy of Cells: Glycolysis and the Krebs Cycle Chapter 6: Protein and DNA Structure Chapter 7: Protein Synthesis Part I: Localization of Protein Translation Chapter 8: Protein Synthesis Part II: The Mechanism of Protein Translation Chapter 9: The Energy of Cells: Oxidative Phosphorylation Chapter 10: The Energy of Cells: The Mechanism of ATP Synthesis Chapter 11: Techniques Chapter 12: Cell Signaling Part I: The role of Phosphorylation Chapter 13: Cell Signaling Part II: G-protein-coupled Receptors Chapter 14: The Secretory Pathway Chapter 15: The Mechanism of Cell Death Chapter 16: The Biology of Cancer Index
£47.49
CRC Press A First Course in Systems Biology
A First Course in Systems Biology, Third Edition is an introduction to the growing field of systems biology for advanced undergraduates and graduate students. Its focus is the design and analysis of computational models and their applications to diverse biomedical phenomena, from simple networks and kinetics to complex pathway systems, signal transduction, personalized medicine, and interacting populations. The book begins with the fundamentals of computational modeling, then reviews features of the molecular inventories that bring biological systems to life and ends with case studies that reflect some of the frontiers in systems biology. In this way, the First Course provides the reader with a comprehensive background and with access to methods for executing standard tasks of biomedical systems analysis, exposure to the modern literature, and a foundation for launching into specialized projects that address biomedical questions with theoretical and computati
£71.24
Springer-Verlag New York Inc. Pericytes
Book SynopsisTable of ContentsPreface…Table of Contents…Contributing Authors…1. Visualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture Roshini Prakash, Nikita Shivani Thareja, Thomas S. Carmichael, Raymond L. Barnhill, Claire Lugassy, and Laurent A. Bentolila2. Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu MiceJames E. Baily and William C.W. Chen3. Assessment of Pericyte Phenotype by Flow CytometryN. S. Khan, C.C. West, F. Rossi, and M. Crisan4. Characterization of Pericyte Phenotype In Vivo by ImmunohistochemistryC.C. West, N.S. Khan, and M. Crisan5. A Metabolomics Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures E.V. Alakpa, C.C. West, L. Goldie, M. Harper, K. Burgess, R. Ulijn, and M.J. Dalby6. Pericyte Ontogeny: The Use of Chimeras to Track a Cell Lineage of Diverse Germ Line OriginsHeather C. Etchevers7. Imaging Pericytes and the Regulation of Cerebral Blood FlowKatie Boyd, Matthew Hammond-Haley, Rozan Vroman, and Catherine Hall8. Derivation of Pericytes from Human Pluripotent Stem CellsAyelet Dar and Joseph Itskovitz-Eldor9. Assessing the Bone-Forming Potential of Pericytes Carolyn A. Meyers, Chenchao Wang, Jiajia Xu, Hsin Chuan Pan, Jia Shen, Kang Ting, Chia Soo, Bruno Peault, and Aaron W. James10. Manipulating Pericyte Function with MicroRNAsMilena Vitiello, Ben Cathcart, Andrea Caporali, and Marco Meloni11. Isolation of Mesoangioblasts: A Subset of Pericytes with Strong Myogenic Potential from Mammals Giorgia Giacomazzi, Gaia Giovannelli, Alessio Rotini, Domiziana Costamagna, Mattia Quattrocelli, and Maurilio Sampaolesi12. Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes Ania Stefanska, Angela Briski, Nusrat Khan, Christopher Kenyon, John J. Mullins, and Bruno Péault13. Metastatic Dissemination Mimicked in a Multicellular Transwell AssayCourtney König and Anja RungeSubject Index List…
£98.99
Springer-Verlag New York Inc. cAMP Signaling
Book SynopsisThis volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, cAMP Signaling: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers who are interested in learTable of ContentsPreface…Table of Contents...Contributing Authors…1. Real-Time Measurements of Intracellular cAMP Gradients using FRET-Based cAMP NanorulersCharlotte Konrad, Martin J. Lohse, and Andreas Bock2. Assaying Protein Kinase A Activity using a FRET-Based Sensor Purified from Mammalian CellsAshton J. Curtis, Ryan S. Dowsell, and Matthew G. Gold3. MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRETMasoud Ramuz, Ivan Diakonov, Chris Dunsby, and Julia Gorelik4. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal ActivityThilo Henss, Martin Schneider, Dennis Vettkötter, Wagner Steuer Costa, Jana F. Liewald, and Alexander Gottschalk5. Imaging the cAMP Signaling Microdomain of the Primary Cilium using Targeted FRET-Based BiosensorsDanielle T. Arena and Aldebaran M. Hofer6. Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity via Heterologous Expression in Fission YeastMarek Domin and Charles S. Hoffman7. Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET SensorsOlga Kukk, Jeffrey Klarenbeek, and Kees Jalink8. Disruptors of AKAP-Dependent Protein-Protein InteractionsRyan Walker-Gray, Tamara Pallien, Duncan C. Miller, Andreas Oder, Martin Neuenschwander, Jens Peter von Kries, Sebastian Diecke, and Enno Klussmann9. Micro-2D Cell Culture for cAMP Measurements using FRET Reporters in Human iPSC-Derived Cardiomyocytes Andreas Koschinski and Manuela Zaccolo10. Automated Image Analysis of FRET Signals for Subcellular cAMP QuantificationSilas J. Leavesley, Naga Annamdevula, Santina Johnson, DJ Pleshinger, and Thomas C. Rich11. In Vivo cAMP Dynamics in Drosophila Larval NeuronsIsabella Maiellaro12. Live Cell Imaging of Cyclic Nucleotides in Human CardiomyocytesKira Beneke and Cristina E. Molina13. Optogenetic Control of Heart Rhythm: Lightly Guiding the Cardiac PaceLolita Dokshokova, Nicola Pianca, Tania Zaglia, and Marco Mongillo14. Live Imaging of cAMP Signaling in D. discoideum Based on a Bright Bioluminescent Protein, Nano-LanternKazuki Horikawa and Takeharu Nagai15. Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMPRoberta Kurelić and Viacheslav O. Nikolaev16. How to Make the CUTiest Sensor in 3 Simple Steps for Computational PedestriansFlorencia Klein, Cecilia Abreu, and Sergio Pantano17. Ion-Channel Based Reporters for cAMP DetectionThomas C. Rich, Wenkuan Xin, Silas J. Leavesley, C. Michael Francis, and Mark Taylor18. Quantitative Phosphoproteomics to Study cAMP SignallingKatharina Schleicher, Svenja Hester, Monika Stegmann, and Manuela Zaccolo 19. Biochemical Analysis of AKAP-Anchored PKA Signaling ComplexesDominic P. Byrne, Mitchell H. Omar, Eileen J. Kennedy, Patrick A. Eyers, and John D. Scott20. Fluorescent Translocation Reporters for Sub-Plasma Membrane cAMP ImagingOleg Dyachok, Yunjian Xu, Olof Idevall-Hagren, and Anders Tengholm21. A Live-Cell Imaging Assay for Nuclear Entry of cAMP-Dependent Protein Kinase Catalytic Subunits Stimulated by Endogenous GPCR Activation Grace E. Peng and Mark von Zastrow22. Measuring Spatiotemporal cAMP Dynamics within an Endogenous Signaling Compartment using FluoSTEP-ICUEJulia C. Hardy, Sohum Mehta, and Jin ZhangSubject Index List…
£143.99
Springer-Verlag New York Inc. Celebrating J.D. Murrays Contributions to
Book SynopsisThe Bulletin of Mathematical Biology, the official journal of the Society for Mathematical Biology, disseminates original research findings and other information relevant to the interface of biology and the mathematical sciences. Contributions should have relevance to both fields. In order to accommodate the broad scope of new developments, the journal accepts a variety of contributions, including: Original research articles focused on new biological insights gained with the help of tools from the mathematical sciences or new mathematical tools and methods with demonstrated applicability to biological investigations Research in mathematical biology education Reviews Commentaries Perspectives, and contributions that discuss issues important to the profession All contributions are peer-reviewed.
£80.99
Springer-Verlag New York Inc. The Plant Cytoskeleton
Book SynopsisThis detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.Table of Contents1. Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy Sharol Schmidt-Marcec, Austin Ross, and Andrei Smertenko 2. Actin: Static and Dynamic Studies Huaqiang Ruan, Sha Zhang, Yi Zhang, and Haiyun Ren 3. 3D Visualization of Microtubules in Epidermal Pavement Cells Amir J. Bidhendi, Bara Altartouri, and Anja Geitmann 4. Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells Sandra Klemm, Jonas Buhl, Birgit Möller, and Katharina Bürstenbinder 5. Single Cell Confinement Methods to Study Plant Cytoskeleton Pauline Durand-Smet, Antoine Chevallier, Léia Colin, Alice Malivert, Isaty Melogno, and Olivier Hamant 6. Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures Eduard Belausov, Vikas Dwivedi, Sela Yechezkel, Sefi Bar-Sinai, and Einat Sadot 7. Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography Janice Pennington and Marisa S. Otegui 8. Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana) Yuh-Ru Julie Lee, Calvin H. Huang, and Bo Liu 9. Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis Sabine Müller 10. Expansion Microscopy of Plant Cells (PlantExM) Timothy J. Hawkins, Joanne L. Robson, Bethany Cole, and Simon J. Bush 11. Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens Mari W. Yoshida and Elena Kozgunova 12. Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens Yuji Hiwatashi and Takashi Murata 13. Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools Fatima Cvrčková and Radek Bezvoda 14. Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata Zoe Barr and Jens Tilsner 15. Studying Nuclear Dynamics in Response to Actin Disruption In Planta Joseph F. McKenna and Katja Graumann 16. Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination Lucie Riglet and Isabelle Fobis-Loisy 17. Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes Lei Zhu and Ying Fu 18. Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study Teresa Braga, Fernando Vaz Dias, Marta Fratini, Susana Serrazina, Ingo Heilmann, and Rui Malhó 19. Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis Liru Dou, Xiangfeng Wang, and Tonglin Mao 20. Imaging of Cortical Microtubules in Plants under Salt Stress Shuwei Wang, Liyuan Xu, Changjiang Li, Lei Zhu, Ying Fu, and Yan Guo 21. Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli Bingxiao Wang, Minxia Zou, Qing Pan, and Jiejie Li 22. Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation Amber J. Connerton, Stefan Sassmann, and Michael J. Deeks 23. Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes Qiaonan Lu, Xiaonan Liu, Xiaolu Qu, and Shanjin Huang 24. Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings Felix Ruhnow, Staffan Persson, and René Schneider 25. Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging Guangda Wang, Yanjun Yu, and Zhaosheng Kong 26. Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells Xie Dang, Lilan Zhu, Huibo Ren, and Deshu Lin 27. Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato Zhijing Xu, Xiaolu Qu, Shuang Wu, and Pengwei Wang 28. Light Microscopy Technologies and the Plant Cytoskeleton Timothy J. Hawkins 29. Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton Patrick Duckney and Patrick J. Hussey
£179.99
Springer-Verlag New York Inc. The Bacterial Cell Wall
Book SynopsisBioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall.- Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography.- Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy.- Computational and Biophysical Approaches to Identify Cell Wall-Associated Modulators in Salmonella enterica Serovar Typhi.- Employing Cloning-Independent Mutagenesis of Parvimonas micra for the Study of Cell Wall Biogenesis.- A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum.- Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids.- Type I Lipoteichoic Acid (LTA) Detection by Western Blot.- Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) SpectTable of Contents1. Bioorthogonal Labeling and Click-Chemistry-Based Visualization of the Tannerella forsythia Cell Wall Stephen N. Hyland, Sreedevi Chinthamani, Sushanta Ratna, Kimberly A. Wodzanowski, Liam-Michael D. Sandles, Kiyonobu Honma, Catherine Leimkuhler-Grimes, and Ashu Sharma 2. Probing Membrane-Associated Cytoskeletal Oligomers of the Bacterial Divisome by Electron Microscopy and Tomography Bo Hu and William Margolin 3. Visualization of a Cell Wall Hydrolase Inhibitor in Fusobacterium nucleatum by Immunofluorescence Microscopy Kevin To, Timmie Britton, and Hung Ton-That 4. Computational and Biophysical Approaches to Identify Cell Wall-Associated Modulators in Salmonella enterica Serovar Typhi Mukesh Kumar, Md. Anzarul Haque, and Punit Kaur 5. Employing Cloning-Independent Mutagenesis of Parvimonas micra for the Study of Cell Wall Biogenesis Dustin L. Higashi, Zhengzhong Zou, Hua Qin, Jens Kreth, and Justin Merritt 6. A New Method for Gene Deletion to Investigate Cell Wall Biogenesis in Fusobacterium nucleatum Bibek G.C., Peng Zhou, and Chenggang Wu 7. Super-Resolution Microscopy of the Bacterial Cell Wall Labeled by Fluorescent D-Amino Acids Chen Zhang and Suliana Manley 8. Type I Lipoteichoic Acid (LTA) Detection by Western Blot Charlotte Millership and Angelika Gründling 9. Type I Lipoteichoic Acid (LTA) Purification by Hydrophobic Interaction Chromatography and Structural Analysis by 2D Nuclear Magnetic Resonance (NMR) Spectroscopy Jeanine Rismondo and Angelika Gründling 10. Single-Copy Gene Editing of a Cell Wall-Anchored Pilin in Actinomyces oris Aadil H. Bhat, Asis Das, and Hung Ton-That 11. Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography Nicole Cheung, Mabel Song, Christopher K. Sue, and Robert T. Clubb 12. Detection of Cell Wall-Anchoring Machinery by Immunogold-Labeling Thin-Section Electron Microscopy Chungyu Chang and Hung Ton-That 13. Localization of the Remnant of a Cell Wall Sorting Signal and Its Interaction with a Sensor Kinase Jeffrey W. Hall, Bruno P. Lima, Karen F. Johnstone, and Mark C. Herzberg 14. Determination of the Crystal Structure of the Cell Wall-Anchored Proteins and Pilins Shivangi Tyagi, Rajnesh Kumari Yadav, and Vengadesan Krishnan 15. Tracking Cell Wall Anchored Proteins in Gram-Positive Bacteria Salvatore J. Scaffidi and Wenqi Yu 16. Probing Bacterial Cell Division and Cell Envelope Biogenesis with Live-Cell Fluorescence Microscopy Lauren R. Hammond, Maria L. White, and Prahathees J. Eswara 17. Assembling the Bacillus subtilis Spore Coat Basement Layer on Spherical Supported Lipid Bilayers Taylor B. Updegrove, Domenico D’Atri, and Kumaran Ramamurthi 18. Structural Determination of Glucosyltransferase C by Cryo-Electron Microscopy Qing Xie, Jianhao Cao, Hua Zhang, and Hui Wu
£161.99
Springer-Verlag New York Inc. Borrelia burgdorferi
Book SynopsisTable of ContentsPreface…Table of Contents…Contributing Authors…1. Real-Time Microfluidic PCRs: A High-Throughput Method to Detect 48 or 96 Tick-Borne Pathogens in 48 or 96 SamplesSara Moutailler and Clemence Galon2. Direct Detection of Borrelia Species in TissuesShiva Kumar Goud Gadila and Monica E. Embers3. Purification of Borrelia burgdorferi Outer Membrane VesiclesHanna Tammisto and Kati Karvonen4. Constructing an ELISA for Detection of Anti-Borrelia in Wildlife and Agricultural AnimalsJulia Bland, Caitlin McGowan, Emma Bush, and Vett Lloyd5. A Step-by-Step Guide to Selecting an Optimal Cut-Off Value Based on the Receiver Operating Characteristic and Youden Index in Methods Designed to Diagnose Lyme DiseaseKunal Garg and Sara Campolonghi6. Adaptive Immune Response Investigation in Lyme borreliosisMihail Pruteanu, Armin Schwarzbach, and Markus Berger7. CYTOSPOT: Intracellular Staining and Detection of Cytokines by Flow Cytometer, An Alternative to LYMESPOTLuis Calderilla-Barbosa and Jose Luis Flores-Sevilla8. Use of Specific Borrelia Phages as a New Strategy for Improved Diagnostic TestsJinyu Shan, Ying Jia, and Tatjana Mijatovic9. Identification of Unambiguous Borrelia Peptides in Human Urine Using Affinity Capture and Mass SpectrometryRocio Cornero, Sumaiya Safia Irfan, Silvia Cachaco, Weidong Zhou, Ahana Byne, Marissa Howard, Hope McIntyre, Barbara Birkaya, Lance Liotta, and Alessandra Luchini10. Preparation of Borrelia Infected Mammalian Cells for Helium Ion MicroscopyKati Karvonen11. Establishing a Zebrafish Model for Borrelia Burgdorferi Infection Using Immersion and Microinjection MethodsErica Misner, Min Zhang, and Eva Sapi12. Development of Organoids to Study Infectious Host InteractionsAparajita Lahree and Leona Gilbert13. Content Analysis: An Approach to Exploring the Depiction of Tick-Borne DiseasesStephen M. Croucher14. Applying BERT and ChatGPT for Sentiment Analysis of Lyme Disease in Scientific LiteratureTeo Susnjak15. Building a Binary Classification Machine Learning Model: A Guide to Predicting Participation in a Lyme Disease Program at a Medical InstituteKunal Garg, Liria Mitzuko Fajardo-Yamamoto, Flor Cecilia Rojas-Castro, Teo Susnjak, and Leona Gilbert16. Clinical Algorithm and Diagnostic Tools for Lyme DiseaseGisell García-Bretón, Flor C. Rojas-Castro, Astrid N. Espino-Vázquez, and Liria M. Fajardo-Yamamoto17. Establishing a Pregnancy Lyme Disease BiobankStanley J. Naides18. Developing a Prospective Gestational Lyme Disease StudyGraham McLennan, Suzanne E. Dale, Laura Gillim, Vivian Weinblatt, Robert Wallerstein, and Stanley J. NaidesSubject Index List…
£143.99
Humana The Nuclear Membrane
Book SynopsisLipidomic profiling of isolated nuclei from mice and cultured cells.- Profiling plant nuclear envelope composition using subtractive proteomics.- Ultrastructural visualization of the nuclearenvelope in HeLa cells.- Imaging nuclear envelopes using correlative AFM/fluorescence microscopy.- Fast super-resolution live cell imaging of nuclear membrane-endoplasmic reticulum dynamicsImmunostaining the yeast nuclear membrane for imaging by super-resolution fluorescence microscopy.- Visualizing phosphatidic acid and diacylglycerol at the nuclear envelope in fission yeast.- Measuring molecular mass densities at subcellular resolution using optical diffraction tomography.- Quantification and comparison of protein distribution on nuclear membrane.- Quantifying nuclear shape fluctuations along the cell cycle, with a focus on early mitosis.- Micropost arrays to model ECM fiber obstacles during cell migration in confinement.- Use of nucleoporin-conjugated beads to study the nuclear pore complex assembly on the nuclear membrane.- T4 DNA-induced reconstruction of artificial nuclei in living mouse oocyte.- Preparation of nucleoplasmic extract and its application in DNA end processing.- Liposome floatation assays to study membrane interactions of nucleoporins.- Giant unilamellar vesicles (GUVs) to study membrane interaction of nucleoporins.- One-pot reconstitution of GPCRs into unilamellar vesicles for fluorescence-based phospholipid scramblase activity assay.- Identifying genomic DNA sequences near the nuclear lamina using proximity biotinylation with Ascorbate Peroxidase.- Pairing LaminB1-DamID and Immuno-3D-FISH to resolve and verify peripheral genome organization of adipogenesis.- Measurement of spatial contact map using sequential FISH.
£161.99
Cambridge University Press On Growth and Form
Book SynopsisAnalysing biological processes in their mathematical and physical aspects, this historic work, first published in 1917, has also become renowned for the sheer poetry of its descriptions. It is now available for a wider readership including a foreword by one of today's great populisers of science.Trade Review'Thompson describes, in great detail, how natural organisms evolve in response to the forces of survival to achieve fitness to purpose. Thompson demonstrates the causality of the shaping and design of natural organisms. For me, as a budding architect, this gave a clue to the concept of fitness to purpose: that architecture must evolve not as a formalistic shape-forming, but from a deep understanding of the programmatic, functional material and economic forces that shape it. This means an understanding of the material and structural aspects of a building, the life intended in a building (i.e. its human purpose) and the many forces (climatic and others) that must be responded to in the process. … [the book is] as topical and significant today, going forward, as [it was], for me, 60 years ago.' Moshe Safdie, Architektura KontekstyTable of ContentsIntroduction John Tyler Bonner; 1. Introductory; 2. On magnitude; 3. The forms of cells; 4. The forms of tissues, of cell-aggregates; 5. On spicules and spicular skeletons; 6. The equiangular spiral; 7. The shapes of horns and of teeth or tusks; 8. On form and mechanical efficiency; 9. On the theory of transformations, or the comparison of related forms; 10. Epilogue; Index.
£21.54
Cambridge University Press Cellular Flows
Book SynopsisA cell, whose spatial extent is small compared with a surrounding flow, can develop inside a vortex. Such cells, often referred to as vortex breakdown bubbles, provide stable and clean flame in combustion chambers; they also reduce the lift force of delta wings. This book analyzes cells in slow and fast, one- and two-fluid flows and describes the mechanisms of cell generation: (a) minimal energy dissipation, (b) competing forces, (c) jet entrainment, and (d) swirl decay. The book explains the vortex breakdown appearance, discusses its features, and indicates means of its control. Written in acceptable, non-math-heavy format, it stands to be a useful learning tool for engineers working with combustion chambers, chemical and biological reactors, and delta-wing designs.Table of Contents1. Introduction; 2. Creeping eddies; 3. Two-fluid creeping flows; 4. Formation of cells in thermal convection; 5. Swirl decay mechanisms; 6. Vortex breakdown in a sealed cylinder; 7. Cellular whirlpool flow; 8. Cellular water-spout flow; 9. Cellular flows in vortex devices.
£161.00
Cambridge University Press Understanding Development
Book SynopsisDevelopmental biology is seemingly well understood, with development widely accepted as being a series of programmed changes through which an egg turns into an adult organism, or a seed matures into a plant. However, the picture is much more complex than that: is it all genetically controlled or does environment have an influence? Is the final adult stage the target of development and everything else just a build-up to that point? Are developmental strategies the same in plants as in animals? How do we consider development in single-celled organisms? In this concise, engaging volume, Alessandro Minelli, a leading developmental biologist, addresses these key questions. Using familiar examples and easy-to-follow arguments, he offers fresh alternatives to a number of preconceptions and stereotypes, awakening the reader to the disparity of developmental phenomena across all main branches of the tree of life.Trade Review'Developmental biology has been described as the process by which a fertilized egg is transformed into a multicellular organism. But is it? In this thoughtful and erudite book, Alessandro Minelli forces us to step back and reconsider the subject. Using an astonishing range of examples, from pythons to lichens and from sponges to ciliates, Minelli challenges a series of generalizations and preconceptions. We see how development is not only the process of building adults, why development does not have end-points, how development need not start with a fertilized egg, why we must be careful with the concept of developmental genes, and much more. After reading this book, you might not think about developmental biology in the same way again.' Peter Holland, University of Oxford, UK'This is the finest book on the principles underpinning biological development that I have read in a long time. It is succinct, thoughtful and full of examples, offering wise reflection on the diversity of developmental phenomena across the whole tree of life. Understanding Development is especially notable for its organization into 48 sections comprising 8 chapters. Each section subtitle states a key lesson to be learned through brief historical and theoretical expositions, well-chosen examples, and stories of odd-ball and familiar life forms. Every lesson overturns some conventional wisdom or common knowledge that cannot stand up to the wondrous diversity of life on Earth. Minelli's broad, deep knowledge of the field is expressed with an engaging contrarian spirit that serves his larger goal: to prompt a reassessment of the state of contemporary understanding of development in a way accessible to novice and expert alike.' James Griesemer, University of California, Davis, USA'Developmental biology is a highly dynamic area of the life sciences, and it also lacks a unifying theoretical framework and must rely on general principles derived from a small number of well-studied model organisms. In Understanding Development, Minelli channels an encyclopaedic knowledge of biological diversity to convincingly show the need for a more expansive concept of development that can embrace the variability and complexity of life. Minelli surveys the interplay of generalizations and exceptions that arise in the study of development, tracing out important open conceptual challenges facing researchers today. Engagingly written and always insightful, this book is highly recommended to biologists, philosophers of biology, and historians interested in grappling with a fundamental and active problem area in the contemporary landscape of biological thought.' James DiFrisco, KU Leuven, Belgium'The text assumes a basic acquaintance with evolution, genetics, and embryology and is at once well written, entertaining, and loaded with fascinating examples of organisms that defy expectations … Highly recommended.' J. L. Hunt, Choice MagazineTable of Contents1. Defining development, If possible; 2. Cells and development; 3. Development as the history of the individual; 4. Revisiting the embryo; 5. Developmental sequences: sustainability vs adaptation; 6. Genes and development; 7. Emerging form; 8. The ecology of development; Concluding remarks.
£11.99
John Wiley and Sons Ltd Oxidative Stress in Vertebrates and Invertebrates
Book SynopsisThis volume presents a unique comparative treatment of the role oxidative stress plays in vertebrates and invertebrates in multiple organ systems with regards to cell death, development, aging, and human diseases, and anti-oxidant therapy. It offers comprehensive reviews of the current understanding of oxidative stress-mediated physiology and pathology as well as directions for future research. It also provides current information on the role of oxidative stress in neurodegenerative diseases, cardiovascular diseases, and various types of cancer mediated by oxidative stress.Table of ContentsPREFACE xi Tahira Farooqui and Akhlaq A. Farooqui FOREWORD xiii Grace Y. Sun ACKNOWLEDGMENTS xv Tahira Farooqui and Akhlaq A. Farooqui CONTRIBUTORS xvii PART I OXIDATIVE STRESS IN VERTEBRATES 1 Generation of Reactive Oxygen Species in the Brain: Signaling for Neural Cell Survival or Suicide 3 Akhlaq A. Farooqui 2 Free Radicals, Signal Transduction, and Human Disease 17 Klaudia Jomova and Marian Valko 3 Oxidative Stress and its Biochemical Consequences in Mitochondrial DNA Mutation-Associated Diseases: Implications of Redox Therapy for Mitochondrial Diseases 33 Shi-Bei Wu, Yu-Ting Wu, Yi-Shing Ma, and Yau-Huei Wei 4 Oxidative Stress in Kainic Acid Neurotoxicity: Implications for the Pathogenesis of Neurotraumatic and Neurodegenerative Diseases 51 Akhlaq A. Farooqui 5 Survival Strategy and Disease Pathogenesis According to the Nrf2-Small Maf Heterodimer 63 Masanobu Morita and Hozumi Motohashi 6 Caloric Restriction and Oxidative Stress 83 Jan Škrha 7 Pathogenesis of Neurodegenerative Diseases: Contribution of Oxidative Stress and Neuroinflammation 103 Tahira Farooqui and Akhlaq A. Farooqui 8 Neurosteroids in Oxidative Stress-Mediated Injury in Alzheimer Disease 117 Amandine Grimm, Ayikoe Guy Mensah-Nyagan, and Anne Eckert 9 Oxidative Stress in Adult Neurogenesis and in the Pathogenesis of Alzheimer Disease 129 Philippe Taupin 10 Oxidative Stress and Parkinson Disease 139 Kah-Leong Lim, Doyle Graham, and Xiao-Hui Ng 11 Oxidative Stress in Cardiovascular Diseases 153 Michael E. Andrades and Rodrigo Lorenzi 12 Oxidative Stress and Aging: A Comparison between Vertebrates and Invertebrates 167 Syed Ibrahim Rizvi and Kanti Bhooshan Pandey 13 Oxidative Stress-Mediated Signaling Pathways by Environmental Stressors 175 Hideko Sone and Hiromi Akanuma 14 Selenoproteins in Cellular Redox Regulation and Signaling 195 Arjun V. Raman and Marla J. Berry 15 Antioxidant Therapy and its Effectiveness in Oxidative Stress-Mediated Disorders 209 Tommaso Iannitti and Beniamino Palmieri 16 The Protective Role of Grape Seed Polyphenols Against Oxidative Stress in Treating Neurodegenerative Diseases 235 Giulio Maria Pasinetti 17 Pharmacological and Therapeutic Properties of Propolis (Bee Glue) 245 Kandangath Raghavan Anilakumar, Farhath Khanum, and Amarinder Singh Bawa PART II OXIDATIVE STRESS IN INVERTEBRATES 18 Endocrine Control of Oxidative Stress in Insects 261 Natraj Krishnan and Dalibor Kodri´k 19 Oxidative Stress in the Airway System of the Fruit Fly Drosophila melanogaster 271 Thomas Roeder 20 Molecular Mechanisms of Antioxidant Protective Processes in Honeybee Apis mellifera 279 Alexey G. Nikolenko, Elena S. Saltykova, and Louisa R. Gaifullina 21 Molecular Basis of Iron-induced Oxidative Stress in the Honeybee Brain: A Potential Model System of Olfactory Dysfunction in Neurological Diseases 295 Tahira Farooqui 22 Modulation of Oxidative Stress by Keap1/Nrf2 Signaling in Drosophila: Implications for Human Diseases 309 Gerasimos P. Sykiotis, M. Mahidur Rahman, and Dirk Bohmann 23 Orchestration of Oxidative Stress Responses in Drosophila melanogaster: A Promoter Analysis Study of Circadian Regulatory Motifs 327 Kuntol Rakshit and Natraj Krishnan 24 The Protective Role of Sestrins Against Chronic TOR Activation and Oxidative Stress 337 Jun Hee Lee and Ethan Bier 25 Current Advances in the Studies of Oxidative Stress and Age-Related Memory Impairment in C. elegans 347 Shin Murakami, Kelly Cabana, and Danielle Anderson 26 Oxidative Challenge and Redox Sensing in Mollusks: Effects of Natural and Anthropic Stressors 361 Julie Letendre, Franc¸ois Leboulenger, and Fabrice Durand 27 Perspective and Directions for Future Studies 377 Tahira Farooqui and Akhlaq A. Farooqui INDEX 385
£145.30
John Wiley and Sons Ltd Molecular Biology of Cancer 2e
Book SynopsisThe Molecular Biology of Cancer, Stella Pelengaris & Michael Khan This capturing, comprehensive text, extensively revised and updated for its second edition, provides a detailed overview of the molecular mechanisms underpinning the development of cancer and its treatment. Bench to Bedside: A key strength of this book that sets it apart from general cancer biology references is the interweaving of all aspects of cancer biology from the causes, development and diagnosis through to the treatment and care of cancer patients essential for providing a broader view of cancer and its impact. The highly readable presentation of a complex field, written by an international panel of researchers, specialists and practitioners, would provide an excellent text for graduate and undergraduate courses in the biology of cancer, medical students and qualified practitioners in the field preparing for higher exams, and for researchers and teachers in the field. For thTrade Review“An excellent didactic technical production. For medical students, trainees, cancer biologists, oncologists, pharmacologists and endocrinologists. Cancer remains a menace in modern times.” (Pediatric Endocrinology Reviews, 1 September 2013) “This book will be invaluable for nursing and medical students, establishing a basic understanding and progressing to more complex issues. The end-of-chapter review questions are great preparation for exams.” (Nursing Standard, 1 September 2013Table of ContentsContributors vii Preface to the Second Edition ix Reviews of the First Edition x Acknowledgments and Dedication xi About the Companion Website xii Introduction 1 1 Overview of Cancer Biology 3 Michael Khan and Stella Pelengaris Introduction 5 Cancer incidence and epidemiology 8 Towards a definition of cancer 8 Causes of cancer 16 Cancer is a genetic disease 21 Cancers (and Darwin’s finches) evolve by mutation and natural selection 21 Blame the parents – inherited single gene defects and susceptibility to cancer 21 The cancer “roadmap” – What kinds of genes are epimutated in cancer? 23 Viruses and the beginnings of cancer biology 25 Hens and teeth or bears and woods? The hens have it – cancer is rare 25 The barriers to cancer 25 What is the secret of cancer developme . . . “timing” 28 Location location location – the cancer environment: nanny or spartan state 28 Cancer goes agricultural 29 Cancer superhighways – blood vessels and lymphatics 31 On your bike and turn the lights off before you go 31 Catching cancer 31 Hammering the hallmarks 32 Painting a portrait of cancer 33 The drugs don’t work 34 Mechanism of origin rather than cell of origin – towards a new functional taxonomy of cancer 35 Is it worth it? 36 Conclusions and future directions 36 Bibliography 37 Appendix 1.1 History of cancer 40 2 The Burden of Cancer 43 William P. Steward and Anne L. Thomas Introduction 43 Lung cancer 45 Breast cancer 49 Colorectal cancer 53 Carcinoma of the prostate 56 Renal carcinoma 57 Skin cancer 58 Carcinoma of the cervix 60 Hematological malignancies 60 Conclusions and future directions 63 Outstanding questions 63 Bibliography 64 Questions for student review 66 3 Nature and Nurture in Oncogenesis 67 Michael Khan and Stella Pelengaris Introduction 69 Risk factors 73 Preventing cancers 76 Cancer genetics – in depth 78 Cancer genomics 87 Gene–environment interactions 89 Mutations and treatment 89 Chemoprevention of cancer 90 Risk factors act in combination 90 Environmental causes of cancer 93 The clinical staging and histological examination of cancer 101 Screening and biomarkers 102 Somatic gene mutations epigenetic alterations and multistage tumorigenesis 105 Conclusions and future directions 107 Outstanding questions 107 Bibliography 107 Questions for student review 109 4 DNA Replication and the Cell Cycle 111 Stella Pelengaris and Michael Khan Introduction 112 The cell cycle – overview 114 Phases of the cell cycle 120 The cell-cycle engine: cyclins and kinases 123 Regulation by degradation 126 Regulation by transcription 129 MicroRNAs and the cell cycle 131 Chromatin 131 DNA replication and mitosis 131 Checkpoints – putting breaks on the cell-cycle The DNA damage response (DDR) 136 The checkpoints 136 Cell-cycle entry and its control by extracellular signals 138 Changes in global gene expression during the cell cycle 139 Cell cycle and cancer 139 Drugging the cell cycle in cancer therapies 141 Conclusions and future directions 142 Outstanding questions 143 Bibliography 143 Questions for student review 144 5 Growth Signaling Pathways and the New Era of Targeted Treatment of Cancer 146 Stella Pelengaris and Michael Khan Introduction 147 Growth factor regulation of the cell cycle 150 Growth homeostasis and tissue repair and regeneration 151 Regulated and deregulated growth 155 Cellular differentiation 157 Tissue growth and the “angiogenic switch” 158 Cancers and nutrients 158 Growth factor signaling pathways 160 A detailed description of signal transduction pathways and their subversion in cancer 160 Translational control and growth 184 Conclusions and future directions 185 Outstanding questions 185 Bibliography 186 Questions for student review 187 6 Oncogenes 188 Stella Pelengaris and Michael Khan Introduction 189 The oncogenes 189 The discovery of oncogenes ushers in the new era of the molecular biology of cancer 191 Overview of oncogenes 191 Types of oncogenes 193 Oncogene collaboration – from cell culture to animal models 199 The c-MYC oncogene 199 The RAS superfamily 213 SRC – the oldest oncogene 228 BCR–ABL and the Philadelphia chromosome 232 The BCL-2 family 235 Biologically targeted therapies in cancer and the concept of “oncogene addiction” 235 Conclusions and future directions 235 Outstanding questions 236 Bibliography 236 Questions for student review 238 7 Tumor Suppressors 239 Martine F. Roussel Introduction 239 The “two-hits” hypothesis: loss of heterozygosity (LOH) 240 Haploinsuffi ciency in cancer 240 Epigenetic events 242 Definition of a tumor suppressor 242 The retinoblastoma protein family 242 p53/TP53 250 INK4a/ARF 254 The p53 and RB pathways in cancer 257 Senescence and immortalization: Role of RB and p53 258 Tumor suppressors and the control of cell proliferation 258 Tumor suppressors and control of the DNA damage response and genomic stability 260 The microRNAs and tumor suppressors 260 Conclusions and future directions 263 Acknowledgments 263 Outstanding questions 264 Bibliography 264 Questions for student review 265 8 Cell Death 266 Stella Pelengaris and Michael Khan Introduction 267 An historical perspective 267 Apoptosis in context 267 Apoptosis as a barrier to cancer formation 271 Apoptosis versus necrosis 271 Cell death by necrosis – not just infl ammatory 272 The pathways to apoptosis 272 The apoptosome – “wheel of death” 274 Caspases – the initiators and executioners of apoptosis 274 The IAP family – inhibitors of apoptosis and much more 276 The central role of MOMP and its regulators in apoptosis – the BCL-2 family 279 Mitochondrial outer membrane permeabilization (MOMP) 281 Endoplasmic reticulum stress 282 Stress-inducible heat shock proteins 282 Tumor suppressor p53 282 Oncogenic stress: MYC-induced apoptosis 283 Autophagy – a different kind of cell death and survival 287 Cell death in response to cancer therapy 290 Exploiting cell death (and senescence) in cancer control 290 Conclusions and future directions 292 Outstanding questions 293 Bibliography 293 Questions for student review 294 9 Senescence Telomeres and Cancer Stem Cells 295 Maria A. Blasco and Michael Khan Introduction 296 Senescence 298 Conclusions and future directions 310 Outstanding questions 310 Bibliography 311 Questions for student review 312 10 Genetic Instability Chromosomes and Repair 314 Michael Khan Introduction 316 Telomere attrition and genomic instability 321 Sensing DNA damage 323 Repairing DNA damage 325 Checkpoints 336 Microsatellites and minisatellites 343 Chaperones and genomic instability 344 Cancer susceptibility syndromes involving genetic instability 345 Genomic instability and colon cancer 346 Conclusions and future directions 346 Outstanding questions 347 Bibliography 347 Questions for student review 349 11 There Is More to Cancer than Genetics: Regulation of Gene and Protein Expression by Epigenetic Factors Small Regulatory RNAs and Protein Stability 350 Stella Pelengaris and Michael Khan Introduction 351 The language of epigenetics 353 Epigenetics 353 Methylation of DNA 359 Acetylation of histones and other posttranslational modifications 360 Epigenetics and cancer 362 CIMP and MIN and the “mutator phenotype” 365 Imprinting and loss of imprinting 366 Clinical use of epigenetics 367 Regulation of translation 368 Noncoding RNA and RNA interference 369 Therapeutic and research potential of RNAi 371 Treatments based on miRNA 373 Regulating the proteins 373 Therapeutic inhibition of the proteasome 376 Receptor degradation 377 Wrestling with protein transit – the role of SUMO and the promyelocytic leukemia (PML) body 377 Conclusions and future directions 380 Outstanding questions 380 Bibliography 381 Questions for student review 382 12 Cell Adhesion in Cancer 383 Charles H. Streuli Introduction 383 Adhesive interactions with the extracellular matrix 384 Cell–cell interactions 393 Critical steps in the dissemination of metastases 395 E-cadherin downregulation in cancer leads to migration 399 Epithelial–mesenchymal transitions 401 Integrins metalloproteinases and cell invasion 402 Survival in an inappropriate environment 404 Conclusions 406 Outstanding questions 406 Bibliography 407 Questions for student review 409 13 Tumor Immunity and Immunotherapy 410 Cassian Yee Introduction 410 Endogenous immune response 411 Effector cells in tumor immunity 413 Tumor antigens 417 Antigen-specific therapy of cancer 420 Clinical trials in vaccine therapy 422 Cytokine therapy of cancer 423 Tumor immune evasion 424 Clinical trials in immunomodulatory therapy 425 Conclusions 425 Bibliography 426 Questions for student review 427 14 Tumor Angiogenesis 429 Christiana Ruhrberg Introduction 429 General principles of new vessel growth 430 Pathological neovascularization: tumor vessels 430 Basic concepts in tumor angiogenesis: the angiogenic switch 432 Vascular growth and differentiation factors: stimulators of the angiogenic switch 432 Role of inhibitors in angiogenesis 436 Clinical outcomes and future directions 436 Acknowledgments 437 Bibliography 437 Questions for student review 437 15 Cancer Chemistry: Designing New Drugs for Cancer Treatment 438 Ana M. Pizarro and Peter J. Sadler Introduction 439 Historical perspective 439 The drug discovery process and preclinical development of a drug 442 Questions remaining 457 Conclusions and future directions 457 Bibliography 458 Questions for student review 459 16 Biologically Targeted Agents from Bench to Bedside 461 Michael Khan Peter Sadler Ana M. Pizarro and Stella Pelengaris Introduction 463 Targeted therapies 465 Cancer cell heterogeneity 466 Finding the molecular targets 468 Tumor regression in mice by inactivating single oncogenes 468 Targeted cancer therapies 473 Targeting oncogenes to treat cancer? 473 The concept of synthetic lethality and collateral vulnerability 475 Clinical progress in biological and molecular targeted therapies 476 Molecular targeted drugs – an inventory 479 DNA damage responses 490 Transcription factors 491 Targeting epigenetic regulation of gene expression 492 Hitting the extrinsic support network and preventing spread 493 Gene therapy antisense and siRNA 495 Resistance to targeted therapies – intrinsic resistance and emergence of secondary pathways and tumor escape 497 Negative feedback loops and failure of targeted therapies 500 Biomarkers to identify optimal treatments and tailored therapies 501 Pharmacogenetics and pharmacogenomics 505 Clinical trials in cancer 506 Conclusions and future directions 506 Bibliography 507 Questions for student review 508 17 The Diagnosis of Cancer 509 Anne L. Thomas Bruno Morgan and William P. Steward Introduction 509 Clinical manifestations 510 Investigations in oncological practice 511 Non-invasive imaging techniques 516 Future novel uses of imaging 521 Proteomics and microarrays 523 Circulating tumor cells 523 Disease staging 523 Conclusions and future directions 524 Bibliography 524 Questions for student review 525 18 Treatment of Cancer: Chemotherapy and Radiotherapy 526 Anne L. Thomas J.P. Sage and William P. Steward Introduction 526 Radiotherapy physics 526 Radiobiology 527 Treatment planning 528 Recent advances 529 Chemoradiation 530 Conclusion 540 Bibliography 542 Questions for student review 543 19 Caring for the Cancer Patient 544 Nicky Rudd and Esther Waterhouse Introduction 544 Key concepts 544 Communication with the cancer patient 544 When is palliative care appropriate for cancer patients? 545 Palliative care assessment 545 Symptom control 545 Respiratory symptoms 547 Nausea and vomiting 547 Bowel obstruction 548 Constipation 549 Fatigue 549 Cachexia and anorexia 549 Psychological problems 549 The dying patient 550 Supportive care 550 An example of the care of a cancer patient 551 Questions remaining 551 Conclusions and future directions 551 Underlying problems 551 Comment 551 Underlying problems 552 Bibliography 552 Questions for student review 553 20 Systems Biology of Cancer 554 Walter Schubert Norbert C.J. de Wit and Peter Walden Introduction 556 Information flow in cells 556 Model organisms and cancer models 557 Array-based technologies: genomics epigenomics and transcriptomics 559 SNPs the HapMap and the identification of cancer genes 559 Cancer mRNA expression analysis 562 CGH arrays CpG island microarrays and ChIP-on-Chip 564 Next-generation sequencing 564 Proteomics 566 Posttranslational modifi cations 567 Protein complexes and cellular networks 569 Clinical applications of proteomics 570 Toponomics: investigating the protein network code of cells and tissues 571 Processing the images from the cyclical imaging procedures 571 Structure code and semantics of the toponome: a high-dimensional combinatorial problem 573 Detecting a cell surface protein network code: lessons from a tumor cell 575 The molecular face of cells in diseases 576 Individualized medicine and tailored therapies 576 Discussion and conclusion 579 Bibliography 579 Internet resources 581 Questions for student review 582 Appendix 20.1 Techniques for the generation of genetically altered mouse models of cancer 582 Glossary 585 Answers to Questions 597 Index 603
£83.66
John Wiley & Sons Inc Fluorescent Analogs of Biomolecular Building
Book SynopsisFluorescent Analogs of Biomolecular Building Blocks focuses on the design of fluorescent probes for the four major families of macromolecular building blocks. Compiling the expertise of multiple authors, this book moves from introductory chapters to an exploration of the design, synthesis, and implementation of new fluorescent analogues of biomolecular building blocks, including examples of small-molecule fluorophores and sensors that are part of biomolecular assemblies.Trade Review"This book provides a thorough overview on the design and application of fluorescent analogs of biomolecular building blocks...The way the book is written makes reading enjoyable and relatively easy for readers who already have some knowledge on the subject as well as for beginners...Overall, the book is very well achieved, and I strongly recommend reading." (Angewandte Chemie International Edition May 2017)Table of ContentsList of Contributors xv Preface xix 1 Fluorescence Spectroscopy 1 Renatus W. Sinkeldam, L. Marcus Wilhelmsson, and Yitzhak Tor 1.1 Fundamentals of Fluorescence Spectroscopy 1 1.2 Common Fluorescence Spectroscopy Techniques 3 1.2.1 Steady-State Fluorescence Spectroscopy 3 1.2.2 Time-Resolved Fluorescence Spectroscopy 5 1.2.3 Fluorescence Anisotropy 6 1.2.4 Resonance Energy Transfer and Quenching 7 1.2.5 Fluorescence Microscopy and Single Molecule Spectroscopy 8 1.2.6 Fluorescence-Based in vivo Imaging 9 1.3 Summary and Perspective 10 References 10 2 Naturally Occurring and Synthetic Fluorescent Biomolecular Building Blocks 15 Renatus W. Sinkeldam and Yitzhak Tor 2.1 Introduction 15 2.2 Naturally Occurring Emissive Biomolecular Building Blocks 16 2.3 Synthetic Fluorescent Analogs of Biomolecular Building Blocks 18 2.3.1 Synthetic Emissive Analogs of Membranes Constituents 19 2.3.2 Synthetic Emissive Analogs of Amino Acids 22 2.3.3 Synthetic Emissive Analogs of Nucleosides 24 2.4 Summary and Perspective 31 References 32 3 Polarized Spectroscopy with Fluorescent Biomolecular Building Blocks 40 Bo Albinsson and Bengt Nordén 3.1 Transition Moments 40 3.2 Linear Dichroism 41 3.3 Magnetic Circular Dichroism 45 3.4 F̈orster Resonance Energy Transfer (FRET) 46 3.5 Fluorescence Anisotropy 47 3.6 Fluorescent Nucleobases 47 3.7 Fluorescent Peptide Chromophores 48 3.8 Site-Specific Linear Dichroism (SSLD) 50 3.9 Single-Molecule Fluorescence Resonance Energy Transfer (smFRET) 50 3.10 Single-Molecule Fluorescence-Detected Linear Dichroism (smFLD) 51 References 53 4 Fluorescent Proteins: The Show Must go on! 55 Gregor Jung 4.1 Introduction 55 4.2 Historical Survey 55 4.3 Photophysical Properties 57 4.3.1 Absorption Properties and Color Hue Modification 57 4.3.2 Chromophore Formation 61 4.3.3 Fluorescence Color and Dynamics 64 4.3.4 Directional Properties along with Optical Transitions 68 4.3.5 Energy Transfer and Energy Migration 69 4.4 Photochemical Reactions 71 4.4.1 Excited-state Proton Transfer (ESPT) 71 4.4.2 Isomerization Reactions: Reversible Photoswitching 73 4.4.3 Photoconversion: Irreversible Bond Rupture 74 4.4.4 Other Photochemical Reactions 75 4.5 Ion Sensitivity 75 4.5.1 Ground-State Equilibria of Protonation States 75 4.5.2 Quenching by Small Ions 76 4.6 Relation Microscopy–Spectroscopy for Fluorescent Proteins 77 4.6.1 Brightness Alteration from Cuvette to Microscopic Experiments 77 4.6.2 Lessons from Microspectrometry 78 4.6.3 Tools for Advanced Microscopic Techniques 79 4.7 Prospects and Outlook 82 Acknowledgments 82 References 82 5 Design and Application of Autofluorescent Proteins by Biological Incorporation of Intrinsically Fluorescent Noncanonical Amino Acids 91 Patrick M. Durkin and Nediljko Budisa 5.1 Introduction 91 5.2 Design and Synthesis of Fluorescent Building Blocks in Proteins 97 5.2.1 Extrinsic Fluorescent Labels 97 5.2.2 Intrinsic Fluorescent Labels 98 5.2.3 Extrinsic Labels Chemically Ligated using Cycloaddition Chemistry 108 5.2.4 Modification of the Genetic Code to Incorporate ncAAs 109 5.3 Application of Fluorescent Building Blocks in Proteins 111 5.3.1 Azatryptophans 111 5.3.2 FlAsH-EDT2 Extrinsic Labeling System 112 5.3.3 Huisgen Dipolar Cycloaddition System 114 5.4 Conclusions 117 5.5 Prospects and Outlook 118 5.5.1 Heteroatom-Containing Trp Analogs 119 5.5.2 Expanded Genetic Code – Orthogonal Pairs 119 Acknowledgments 120 References 120 6 Fluoromodules: Fluorescent Dye–Protein Complexes for Genetically Encodable Labels 124 Bruce A. Armitage 6.1 Introduction 124 6.2 Fluoromodule Development and Characterization 126 6.2.1 Fluorogenic Dyes 128 6.2.2 Fluorogen-Activating Protein (FAP) Optimization 131 6.2.3 Fluoromodule Recycling 132 6.3 Implementation 132 6.3.1 Fusion Constructs for Protein Tagging 132 6.3.2 Protein Tagging and pH Sensing 133 6.3.3 Super-Resolution Imaging 133 6.3.4 Protease Biosensors 133 6.4 Conclusions 134 6.5 Prospects and Outlook 134 Acknowledgments 134 References 134 7 Design of Environmentally Sensitive Fluorescent Nucleosides and their Applications 137 Subhendu Sekhar Bag and Isao Saito 7.1 Introduction 137 7.1.1 Solvatochromic Fluorophores 138 7.1.2 Origin of Solvatochromism 139 7.2 Solvatochromic Fluorescent Nucleoside Analogs 140 7.2.1 Designing Criteria for Solvatochromic Fluorescent Nucleosides 140 7.3 Fluorescently Labeled Nucleosides and Oligonucleotide Probes: Covalent Attachment of Solvatochromic Fluorophores Onto the Natural Bases 141 7.3.1 Base-Discriminating Fluorescent Nucleosides (BDF) 142 7.4 Nucleosides with Dual Fluorescence for Monitoring DNA Hybridization 153 7.5 Approach for Developing Environmentally Sensitive Fluorescent (ESF) Nucleosides 154 7.5.1 Concept for Designing ESF Nucleosides 154 7.5.2 Examples and Photophysical Properties of ESF Nucleosides 156 7.6 Base-Selective Fluorescent ESF Probe 163 7.6.1 Cytosine Selective ESF Probe 163 7.6.2 Thymine Selective Fluorescent ESF Probe 163 7.6.3 Specific Detection of Adenine by Exciplex Formation with Donor-Substituted ESF Guanosine 165 7.7 Molecular Beacon (MB) and ESF Nucleosides 167 7.7.1 Ends-Free and Self-Quenched MB 167 7.7.2 Single-Stranded Molecular Beacon Using ESF Nucleoside in a Bulge Structure 168 7.8 Summary and Future Outlook 169 Acknowledgments 170 References 170 8 Expanding The Nucleic Acid Chemist’s Toolbox: Fluorescent Cytidine Analogs 174 Kirby Chicas and Robert H.E. Hudson 8.1 Introduction 174 8.2 Design and Characterization of Fluorescent C Analogs 176 8.2.1 1,3-Diaza-2-Oxophenothiazine (tC) 177 8.2.2 1,3-Diaza-2-Oxophenoxazine (tCO) 178 8.2.3 7-Nitro-1,3-Diaza-2-Oxophenothiazine (tCnitro) 179 8.2.4 G-Clamp and 8-oxoG-Clamp 179 8.2.5 Ç and Çf 181 8.2.6 Benzopyridopyrimidine (BPP) 182 8.2.7 Napthopyridopyrimidine (NPP) 183 8.2.8 dChpp 183 8.2.9 dChpd, dCmpp, dCtpp, dCppp 184 8.2.10 dCPPI 184 8.2.11 dxC 185 8.2.12 rxC 186 8.2.13 Methylpyrrolo-dC (MepdC) 186 8.2.14 5-(Fur-2-yl)-2′-Deoxycytidine (CFU) 187 8.2.15 Thiophen-2-yl pC 187 8.2.16 Thiophene Fused pC 188 8.2.17 Thieno[3,4-d]-Cytidine (thC) 189 8.2.18 Triazole Appended 190 8.3 Implementation 190 8.3.1 PNA 192 8.3.2 DNA 196 8.3.3 RNA 200 8.4 Conclusions 202 8.5 Prospects and Outlook 202 Acknowledgments 203 References 203 9 Synthesis and Fluorescence Properties of Nucleosides with Pyrimidopyrimidine-Type Base Moieties 208 Kohji Seio, Takashi Kanamori, Akihiro Ohkubo, and Mitsuo Sekine 9.1 Introduction 209 9.2 Discovery, Design, and Synthesis of Pyrimidopyrimidine Nucleosides 209 9.2.1 Synthesis and Fluorescence Properties of dChpp 209 9.2.2 Design, Synthesis, and Fluorescence Properties of dCPPP, dCPPI, and dCPPI Derivatives 212 9.2.3 Fluorescence Properties of the Oligonucleotides Containing dCPPI 213 9.3 Implementation 215 9.3.1 Application to a DNA Triplex System 215 9.3.2 Double Labeling of an Oligonucleotide with dCPPI and 2-Aminopurine 219 9.4 Conclusions 220 9.5 Prospects and Outlook 221 References 221 10 Förster Resonance Energy Transfer (FRET) Between Nucleobase Analogues – a Tool for Detailed Structure and Dynamics Investigations 224 L. Marcus Wilhelmsson 10.1 Introduction 224 10.2 The Tricyclic Cytosine Family 226 10.2.1 Structural Aspects, Dynamics, and Ability to Serve as Cytosine Analogs 228 10.2.2 Photophysical Properties 231 10.3 Development of the First Nucleic Acid Base Analog FRET Pair 234 10.3.1 The Donor–Acceptor Pair tCO –tCnitro 235 10.3.2 Applications of Tricyclic Cytosines in FRET Measurements 237 10.4 Conclusions 238 10.5 Prospects and Outlook 238 Acknowledgments 239 References 239 11 Fluorescent Purine Analogs that Shed Light on DNA Structure and Function 242 Anaëlle Dumas, Guillaume Mata, and Nathan W. Luedtke 11.1 Introduction 242 11.2 Design, Photophysical Properties, and Applications of Purine Mimics 244 11.2.1 Early Examples of Fluorescent Purine Mimics 245 11.2.2 Chromophore-Conjugated Purine Analogs 246 11.2.3 Pteridines 250 11.2.4 Isomorphic Purine Analogs 251 11.2.5 Fused-Ring Purine Analogs 252 11.2.6 Substituted Purine Derivatives 253 11.3 Implementation 258 11.3.1 Probing G-Quadruplex Structures with 2PyG 259 11.3.2 Energy Transfer Quantification 261 11.3.3 Metal-Ion Localization to N7 264 11.4 Conclusions 265 11.5 Prospects and Outlook 265 Appendix 268 References 268 12 Design and Photophysics of Environmentally Sensitive Isomorphic Fluorescent Nucleosides 276 Renatus W. Sinkeldam and Yitzhak Tor 12.1 Introduction 276 12.2 Designing Environmentally Sensitive Emissive Nucleosides 279 12.2.1 Structural and Electronic Elements that Impart Environmental Sensitivity 279 12.2.2 Sensitivity to Polarity 279 12.2.3 Sensitivity to Viscosity 281 12.2.4 Sensitivity to pH 282 12.3 Two Isomorphic Environmentally Sensitive Designs 282 12.4 Probing Environmental Sensitivity 283 12.4.1 Probing Sensitivity to Polarity 283 12.4.2 Probing Sensitivity to Viscosity 286 12.4.3 Probing Sensitivity to pH 288 12.5 Recent Advancements in Isomorphic Fluorescent Nucleoside Analogs 291 12.6 Summary 293 12.7 Prospects and Outlook 294 Acknowledgments 294 References 294 13 Site-Specific Fluorescent Labeling of Nucleic Acids by Genetic Alphabet Expansion Using Unnatural Base Pair Systems 297 Michiko Kimoto, Rie Yamashige, and Ichiro Hirao 13.1 Introduction 297 13.2 Development of Unnatural Base Pair Systems and Their Applications 299 13.2.1 Site-Specific Fluorescent Labeling of DNA by Unnatural Base Pair Replication Systems 301 13.2.2 Site-Specific Fluorescent Labeling of RNA by Unnatural Base Pair Transcription Systems 307 13.3 Implementation 310 13.3.1 Fluorescence Sensor System Using an RNA Aptamer by Fluorophore-Linked y Labeling 310 13.3.2 Local Structure Analyses of Functional RNA Molecules by s Labeling 313 13.4 Conclusions 315 13.5 Prospects and Outlook 316 Acknowledgments 317 References 317 14 Fluorescent C-Nucleosides and their Oligomeric Assemblies 320 Pete Crisalli and Eric T. Kool 14.1 Introduction 320 14.2 Design, Synthesis, Characterization, and Properties of Fluorescent C-Glycoside Monomers 322 14.2.1 Design of Fluorescent C-Glycoside Monomers 322 14.2.2 Synthesis of Fluorescent C-Glycoside Monomers 323 14.2.3 Characterization and Properties of Fluorescent C-glycoside Monomers 325 14.3 Implementation of Fluorescent C-Glycoside Monomers 327 14.3.1 Environmentally Sensitive Fluorophores 327 14.3.2 Pyrene Nucleoside in DNA Applications 330 14.4 Oligomers of Fluorescent C-Glycosides: Design, Synthesis, and Properties 335 14.4.1 Design of Fluorescent C-Glycoside Oligomers 335 14.4.2 Synthesis of Fluorescent C-Glycoside Oligomers 336 14.4.3 Characterization and Properties of Fluorescent C-Glycoside Oligomers 337 14.5 Implementation of Fluorescent C-Glycoside Oligomers 342 14.5.1 ODFs as Chemosensors in the Solution State 342 14.5.2 ODFs as Sensors in the Solid State 347 14.5.3 Alternative Designs of Oligomeric Fluorescent Glycosides 351 14.5.4 General Conclusions: Oligomers of Fluorescent C-glycosides 352 14.6 Conclusions 353 14.7 Prospects and Outlook 353 Acknowledgments 354 References 354 15 Membrane Fluorescent Probes: Insights and Perspectives 356 Amitabha Chattopadhyay, Sandeep Shrivastava, and Arunima Chaudhuri Abbreviations 356 15.1 Introduction 357 15.2 NBD-Labeled Lipids: Monitoring Slow Solvent Relaxation in Membranes 358 15.3 n-AS Membrane Probes: Depth-Dependent Solvent Relaxation as Membrane Dipstick 359 15.4 Pyrene: a Multiparameter Membrane Probe 362 15.5 Conclusion and Future Perspectives 362 Acknowledgments 364 References 364 16 Lipophilic Fluorescent Probes: Guides to the Complexity of Lipid Membranes 367 Marek Cebecauer and Radek Šachl 16.1 Introduction 367 16.2 Lipids, Lipid Bilayers, and Biomembranes 368 16.3 Lipid Phases, Phase Separation, and Lipid Ordering 370 16.4 Fluorescent Probes for Membrane Studies 370 16.4.1 Fluorescently Labeled Lipids 371 16.4.2 Environment-Sensitive Membrane Probes 373 16.4.3 Specialized Techniques Using Fluorescent Probes to Investigate Membrane Properties 380 16.5 Conclusions 386 16.6 Prospects and Outlook 386 Acknowledgments 386 References 387 17 Fluorescent Neurotransmitter Analogs 393 James N. Wilson 17.1 Introduction 393 17.1.1 Structure of Neurotransmitters 393 17.1.2 Regulation of Neurotransmitters 394 17.1.3 Native Fluorescence of Neurotransmitters 395 17.1.4 Fluorescent Histochemical Techniques 396 17.2 Design and Optical Properties of Fluorescent Neurotransmitters 397 17.2.1 Early Examples 397 17.2.2 Recent Examples 398 17.3 Applications of Fluorescent Neurotransmitters 400 17.3.1 Probing Binding Pockets with Fluorescent Neurotransmitters 400 17.3.2 Imaging Transport and Release of Fluorescent Neurotransmitters 401 17.3.3 Enzyme Substrates 403 17.4 Conclusions 404 17.5 Prospects and Outlook 405 Acknowledgments 405 References 406 Index 409
£136.76
John Wiley and Sons Ltd Plant Cell Wall Patterning and Cell Shape
Book SynopsisPlant Cell Wall Patterning and Cell Shape is among the first books to take a holistic look at the cell wall?s complex role in plant growth and development. This study examines how cell walls dictate cell shape, their effects on plant development, and researchers? latest perceptions of cell wall pattering.Table of ContentsList of Contributors vii Preface xiii Section 1 Factors Controlling Plant Cell Wall Patterning 1 1 The Biosynthesis and Function of Polysaccharide Components of the Plant Cell Wall 3 Ryusuke Yokoyama, Naoki Shinohara, Rin Asaoka, Hideki Narukawa and Kazuhiko Nishitani 2 Regulation of Cell Wall Formation by Membrane Traffic 35 Clara Sánchez-Rodríguez and Staffan Persson 3 A Blueprint for Cellulose Biosynthesis, Deposition, and Regulation in Plants 65 Ian S. Wallace and Chris R. Somerville 4 Cortical Microtubule Array Organization and Plant Cell Morphogenesis 97 Sidney L. Shaw and Laura Vineyard 5 Actin Filament Dynamics and their Role in Plant Cell Expansion 127 Jiejie Li, Ruthie Arieti and Christopher J. Staiger Section 2 Cellular Mechanisms Underlying Various Cell Shapes 163 6 The Regulation of Cell Shape Formation by ROP-dependent Auxin Signaling 165 Shingo Nagawa and Zhenbiao Yang 7 Xylem Cell Wall Pattern Formation Regulated by Microtubule-associated Proteins and ROP GTPases 191 Yoshihisa Oda and Hiroo Fukuda 8 ROP Signaling and the Cytoskeleton in Pollen Tube Growth 215 Lei Zhu and Ying Fu 9 Phosphoinositide Signaling in Root Hair Tip Growth 239 Hiroaki Kusano, Rumi Tominaga, Takuji Wada, Mariko Kato and Takashi Aoyama 10 Arabidopsis Trichome Morphogenesis and the Role of Microtubules in Controlling Trichome Branch Formation 269 M. David Marks 11 Transfer Cells: Novel Cell Types with Unique Wall Ingrowth Architecture Designed for Optimized Nutrient Transport 287 David W. McCurdy Section 3 Developmental Regulations of Cell Shape 319 12 Regulation of Guard Cell Formation by Integration of Transcriptional and Signaling Regulation 321 Chin-Min Kimmy Ho and Dominique C. Bergmann 13 Transcriptional Regulation of Biosynthesis of Cell Wall Components during Xylem Differentiation 351 Ruiqin Zhong and Zheng-Hua Ye 14 Phloem Cell Development 379 Raffael Lichtenberger, Kaori Furuta-Miyashima, Eva Hellmann and Ykä Helariutta Index 401
£161.06
John Wiley and Sons Ltd Technology Platforms for 3D Cell Culture
Book SynopsisTechnology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs.Table of ContentsList of contributors, vii Preface, xi List of abbreviations, xiv 1 An introduction to the third dimension for routine cell culture, 1Antonio Romo‐Morales and Stefan Przyborski Part I: Aggregate‐based technologies 2 Gravity‐enforced microtissue engineering, 23Randy Strube, Johannes Haugstetter, Markus Furter, Andreia Fernandez, David Fluri and Jens M. Kelm 3 Physiologically relevant spheroid models for three‐dimensionalcell culture, 50Nicole A. Slawny and MaryAnn Labant 4 NanoCulture Plate: A scaffold‐based high‐throughput three‐dimensional cell culture system suitable for live imaging and co‐culture, 74Manabu Itoh, Kazuya Arai, Hiromi Miura and M. Mamunur Rahman 5 Micro‐moulded non‐adhesive hydrogels to form multicellular microtissues – the 3D Petri Dish®, 97Elizabeth Leary, Sean Curran, Michael Susienka, Kali L. Manning, Andrew M. Blakely and Jeffrey R. Morgan 6 Organotypic microtissues on an air‐liquid interface, 123Lars E. Sundstrom, Igor Charvet and Luc Stoppini Part II: Hydrogels 7 Materials and assay systems used for three‐dimensional cell culture, 145Suparna Sanyal and Marshall Kosovsky 8 HyStem®, a customisable hyaluronan‐based hydrogel matrix for 3D cell culture, 173T. I. Zarembinski, B. J. Engel, N. J. Doty, P. E. Constantinou, M. V. Onorato, I. E. Erickson, E. L. S. Fong, M. Martinez, R. L. Milton, B. P. Danysh, N. A. Delk, D. A. Harrington, M. C. Farach‐Carson and D. D. Carson 9 3‐D Life biomimetic hydrogels: A modular system for cell environment design, 197Brigitte M. Angres and Helmut Wurst Part III: Scaffolds 10 Alvetex®, a highly porous polystyrene scaffold for routine three‐dimensional cell culture, 225Antonio Romo‐Morales, Eleanor Knight and Stefan Przyborski 11 CelluSponge™ and Go Matrix as innovative three‐dimensional cell culture platforms, 250Bramasta Nugraha 12 Mimetix® electrospun scaffold: An easy‐to‐use tool for 3D cell culture in drug discovery and regenerative medicine, 284Robert J. McKean and Elena Heister Part IV: 3D bioreactor technologies 13 Quasi Vivo® bioreactor technology, 305J. Malcolm Wilkinson 14 Three‐dimensional cell‐based assays in hollow fibre bioreactors, 327John J. S. Cadwell and William G. Whitford 15 Three‐dimensional engineered tissues for high‐throughput compound screening: Mechanical properties of skin and ageing, 351Michael Conway, Ayla Annac and Tetsuro Wakatsuki 16 Three‐dimensional cell culture in the Rotary Cell Culture System™, 370Stephen S. Navran Glossary, 386 Index, 393
£111.56
John Wiley and Sons Ltd Fundamental Molecular Biology
Book SynopsisFundamental Molecular Biology Discover a focused and up to date exploration of foundational and core concepts in molecular biologyThe newly revised Third Edition of Fundamental Molecular Biology delivers a selective and precise treatment of essential topics in molecular biology perfect for allowing students to develop an accurate understanding of the applications of the field. The book applies the process of discovery-observations, questions, experimental designs, results, and conclusions-with an emphasis on the language of molecular biology. Readers will easily focus on the key ideas they need to succeed in any introductory molecular biology course.Fundamental Molecular Biology provides students with the most up to date techniques and research used by molecular biologists today. Readers of the book will have the support and resources they need to develop a concrete understanding of core and foundational concepts of molecular biology, without beiTable of ContentsChapter 1 The Beginnings of Molecular Biology Chapter 2 The Structure of DNA Chapter 3 The Versatility of RNA Chapter 4 Protein Structure and Folding Chapter 5 Genome Organization and Evolution Chapter 6 DNA Replication and Telomer Maintenance Chapter 7 DNA Repair Pathways Chapter 8 Transcription in Bacteria Chapter 9 Transcription in Eukaryotes Chapter 10 Epigenetic Mechanisms of Gene Regulation Chapter 11 RNA Processing and Posttranscriptional Gene Regulation Chapter 12 The Mechanisms of Translation Chapter 13 Recombinant DNA Technology and Genetically Modified Organisms Chapter 14 Tools for Analyzing Gene Organization, Expression, and Function Chapter 15 Medical Molecular Biology
£130.45
John Wiley & Sons Inc Advanced Surfaces for Stem Cell Research
Book SynopsisThe book outlines first the importance of Extra Cellular Matrix (ECM), which is a natural surface for most of cells. In the following chapters the influence of biological, chemical, mechanical, and physical properties of surfaces in micro and nano-scale on stem cell behavior are discussed including the mechanotransduction. Biomimetic and bioinspired approaches are highlighted for developing microenvironment of several tissues, and surface engineering applications are discussed in tissue engineering, regenerative medicine and different type of biomaterials in various chapters of the book. This book brings together innovative methodologies and strategies adopted in the research and development of Advanced Surfaces in Stem Cell Research. Well-known worldwide researchers deliberate subjects including: Extracellular matrix proteins for stem cell fateThe superficial mechanical and physical properties of matrix microenvironment as stem cell fate regulatorEffects of mechanotransduction on sTable of ContentsPreface xv 1 Extracellular Matrix Proteins for Stem Cell Fate 1 Betül Çelebi-Saltik 1.1 Human Stem Cells, Sources, and Niches 2 1.2 Role of Extrinsic and Intrinsic Factors 5 1.2.1 Shape 5 1.2.2 Topography Regulates Cell Fate 6 1.2.3 Stiffness and Stress 6 1.2.4 Integrins 7 1.2.5 Signaling via Integrins 9 1.3 Extracellular Matrix of the Mesenchyme: Human Bone Marrow 11 1.4 Biomimetic Peptides as Extracellular Matrix Proteins 13 References 15 2 The Superficial Mechanical and Physical Properties of Matrix Microenvironment as Stem Cell Fate Regulator 23 Mohsen Shahrousvand, Gity Mir Mohamad Sadeghi and Ali Salimi 2.1 Introduction 24 2.2 Fabrication of the Microenvironments with Different Properties in Surfaces 25 2.3 Effects of Surface Topography on Stem Cell Behaviors 28 2.4 Role of Substrate Stiffness and Elasticity of Matrix on Cell Culture 31 2.5 Stem Cell Fate Induced by Matrix Stiffness and Its Mechanism 32 2.6 Competition/Compliance between Matrix Stiffness and Other Signals and Their Effect on Stem Cells Fate 33 2.7 Effects of Matrix Stiffness on Stem Cells in Two Dimensions versus Three Dimensions 34 2.8 Effects of External Mechanical Cues on Stem Cell Fate from Surface Interactions Perspective 34 2.9 Conclusions 35 Acknowledgments 36 References 36 3 Effects of Mechanotransduction on Stem Cell Behavior 43 Bahar Bilgen and Sedat Odabas 3.1 Introduction 43 3.2 The Concept of Mechanotransduction 45 3.3 The Mechanical Cues of Cell Differentiation and Tissue Formation on the Basis of Mechanotransduction 46 3.4 Mechanotransduction via External Forces 47 3.4.1 Mechanotransduction via Bioreactors 48 3.4.2 Mechanotransduction via Particle-based Systems 51 3.4.3 Mechanotransduction via Other External Forces 53 3.5 Mechanotransduction via Bioinspired Materials 54 3.6 Future Remarks and Conclusion 54 Declaration of Interest 55 References 55 4 Modulation of Stem Cells Behavior Through Bioactive Surfaces 65 Eduardo D. Gomes, Rita C. Assunção-Silva, Nuno Sousax, Nuno A. Silva and António J. Salgado 4.1 Lithography 66 4.2 Micro and Nanopatterning 70 4.3 Microfluidics 71 4.4 Electrospinning 71 4.5 Bottom-up/Top-down Approaches 74 4.6 Substrates Chemical Modifications 75 4.6.1 Biomolecules Coatings 76 4.6.2 Peptide Grafting 77 4.7 Conclusion 78 References 79 Contents vii 5 Influence of Controlled Micro- and Nanoengineered Environments on Stem Cell Fate 85 Anna Lagunas, David Caballero and Josep Samitier 5.1 Introduction to Engineered Environments for the Control of Stem Cell Differentiation 86 5.1.1 Stem Cells Niche In Vivo: A Highly Dynamic and Complex Environment 86 5.1.2 Mimicking the Stem Cells Niche In Vitro: Engineered Biomaterials 88 5.2 Mechanoregulation of Stem Cell Fate 89 5.2.1 From In Vivo to In Vitro: Influence of the Mechanical Environment on Stem Cell Fate 89 5.2.2 Regulation of Stem Cell Fate by Surface Roughness 90 5.2.3 Control of Stem Cell Differentiation by Micro- and Nanotopographic Surfaces 92 5.2.4 Physical Gradients for Regulating Stem Cell Fate 96 5.3 Controlled Surface Immobilization of Biochemical Stimuli for Stem Cell Differentiation 100 5.3.1 Micro- and Nanopatterned Surfaces: Effect of Geometrical Constraint and Ligand Presentation at the Nanoscale 100 5.3.2 Biochemical Gradients for Stem Cell Differentiation 107 5.4 Three-dimensional Micro- and Nanoengineered Environments for Stem Cell Differentiation 112 5.4.1 Three-dimensional Mechanoregulation of Stem Cell Fate 113 5.4.2 Three-dimensional Biochemical Patterns for Stem Cell Differentiation 119 5.5 Conclusions and Future Perspectives 122 References 122 6 Recent Advances in Nanostructured Polymeric Surface: Challenges and Frontiers in Stem Cells 141 Ilaria Armentano, Samantha Mattioli, Francesco Morena, Chiara Argentati, Sabata Martino, Luigi Torre and Josè Maria Kenny 6.1 Introduction 142 6.2 Nanostructured Surface 144 6.3 Stem Cell 146 6.4 Stem Cell/Surface Interaction 147 6.5 Microscopic Techniques Used in Estimating Stem Cell/Surface 148 6.5.1 Fluorescence Microscopy 148 6.5.2 Electron Microscopy 149 6.5.3 Atomic Force Microscopy 153 6.5.3.1 Instrument 154 6.5.3.2 Cell Nanomechanical Motion 156 6.5.3.3 Mechanical Properties 156 6.6 Conclusions and Future Perspectives 158 References 158 7 Laser Surface Modification Techniques and Stem Cells Applications 165 Çağrı Kaan Akkan 7.1 Introduction 166 7.2 Fundamental Laser Optics for Surface Structuring 166 7.2.1 Definitive Facts for Laser Surface Structuring 167 7.2.1.1 Absorptivity and Reflectivity of the Laser Beam by the Material Surface 167 7.2.1.2 Effect of the Incoming Laser Light Polarization 168 7.2.1.3 Operation Mode of the Laser 169 7.2.1.4 Beam Quality Factor 170 7.2.1.5 Laser Pulse Energy/Power 171 7.2.2 Ablation by Laser Pulses 172 7.2.2.1 Focusing the Laser Beam 172 7.2.2.2 Ablation Regime 173 7.3 Methods for Laser Surface Structuring 174 7.3.1 Physical Surface Modifications by Lasers 174 7.3.1.1 Direct Structuring 175 7.3.1.2 Beam Shaping Optics 177 7.3.1.3 Direct Laser Interference Patterning 180 7.3.2 Chemical Surface Modification by Lasers 181 7.3.2.1 Pulsed Laser Deposition 181 7.3.2.2 Laser Surface Alloying 184 7.3.2.3 Laser Surface Oxidation and Nitriding 186 7.4 Stem Cells and Laser-Modified Surfaces 187 7.5 Conclusions 191 References 192 8 Plasma Polymer Deposition: A Versatile Tool for Stem Cell Research 197 M. N. Macgregor-Ramiasa and K. Vasilev 8.1 Introduction 197 8.2 The Principle and Physics of Plasma Methods for Surface Modification 199 8.2.1 Plasma Sputtering, Etching an Implantation 200 8.2.2 Plasma Polymer Deposition 201 8.3 Surface Properties Influencing Stem Cell Fate 202 8.3.1 Plasma Methods for Tailored Surface Chemistry 203 8.3.1.1 Oxygen-rich Surfaces 204 8.3.1.2 Nitrogen-rich Surfaces 208 8.3.1.3 Systematic Studies and Copolymers 210 8.3.2 Plasma for Surface Topography 211 8.3.3 Plasma for Surface Stiffness 213 8.3.4 Plasma for Gradient Substrata 215 8.3.5 Plasma and 3D Scaffolds 218 8.4 New Trends and Outlook 219 8.5 Conclusions 219 References 220 9 Three-dimensional Printing Approaches for the Treatment of Critical-sized Bone Defects 231 Sara Salehi, Bilal A. Naved and Warren L. Grayson 9.1 Background 232 9.1.1 Treatment Approaches for Critical-sized Bone Defects 232 9.1.2 History of the Application of 3D Printing to Medicine and Biology 233 9.2 Overview of 3D Printing Technologies 234 9.2.1 Laser-based Technologies 235 9.2.1.1 Stereolithography 235 9.2.1.2 Selective Laser Sintering 236 9.2.1.3 Selective Laser Melting 236 9.2.1.4 Electron Beam Melting 237 9.2.1.5 Two-photon Polymerization 237 9.2.2 Extrusion-based Technologies 238 9.2.2.1 Fused Deposition Modeling 238 9.2.2.2 Material Jetting 238 9.2.3 Ink-based Technologies 239 9.2.3.1 Inkjet 3D Printing 239 9.2.3.2 Aerosol Jet Printing 239 9.3 Surgical Guides and Models for Bone Reconstruction 240 9.3.1 Laser-based Surgical Guides 240 9.3.2 Extrusion-based Surgical Guides 240 9.3.3 Ink-based Surgical Guides 241 9.4 Three-dimensionally Printed Implants for Bone Substitution 242 9.4.1 Laser-based Technologies for Metallic Bone Implants 244 9.4.2 Extrusion-based Technologies for Bone Implants 245 9.4.3 Ink-based Technologies for Bone Implants 246 9.5 Scaffolds for Bone Regeneration 246 9.5.1 Laser-based Printing for Regenerative Scaffolds 247 9.5.2 Extrusion-based Printing for Regenerative Scaffolds 247 9.5.3 Ink-based Printing for Regenerative Scaffolds 249 9.5.4 Pre- and Postprocessing Techniques 250 9.5.4.1 Preprocessing 250 9.5.4.2 Postprocessing: Sintering 256 9.5.4.3 Postprocessing: Functionalization 256 9.6 Bioprinting 257 9.7 Conclusion 262 List of Abbreviation 263 References 264 10 Application of Bioreactor Concept and Modeling Techniques to Bone Regeneration and Augmentation Treatments 277 Oscar A. Deccó and Jésica I. Zuchuat 10.1 Bone Tissue Regeneration 278 10.1.1 Proinflammatory Cytokines 279 10.1.2 Transforming Growth Factor Beta 279 10.1.3 Angiogenesis in Regeneration 280 10.2 Actual Therapeutic Strategies and Concepts to Obtain an Optimal Bone Quality and Quantity 281 10.2.1 Guided Bone Regeneration Based on Cells 282 10.2.1.1 Embryonic Stem Cells 282 10.2.1.2 Adult Stem Cells 282 10.2.1.3 Mesenchymal Stem Cells 283 10.2.2 Guided Bone Regeneration Based on PRP and Growth Factors 284 10.2.2.1 Bone Morphogenetic Proteins 287 10.2.3 Guided Bone Regeneration Based on Barrier Membranes 288 10.2.4 Guided Bone Regeneration Based on Scaffolds 290 10.3 Bioreactors Employed for Tissue Engineering in Guided Bone Regeneration 291 10.4 Bioreactor Concept in Guided Bone Regeneration and Tissue Engineering: In Vivo Application 294 10.5 New Multidisciplinary Approaches Intended to Improve and Accelerate the Treatment of Injured and/or Diseased Bone 303 10.5.1 Application of Bioreactor in Dentistry: Therapies for the Treatment of Maxillary Bone Defects 304 10.5.2 Application of Bioreactor in Cases of Osteoporosis 307 10.6 Computational Modeling: An Effective Tool to Predict Bone Ingrowth 310 References 311 11 Stem Cell-based Medicinal Products: Regulatory Perspectives 321 DenizOzdil and Halil Murat Aydin 11.1 Introduction 321 11.2 Defining Stem Cell-based Medicinal Products 323 11.3 Regional Regulatory Issues for Stem Cell Products 326 11.4 Regulatory Systems for Stem Cell-based Technologies 327 11.4.1 The US Regulatory System 328 11.5 Stem Cell Technologies: The European Regulatory System 336 References 340 12 Substrates and Surfaces for Control of Pluripotent Stem Cell Fate and Function 341 Akshaya Srinivasan, Yi-Chin Toh, Xian Jun Loh and Wei Seong Toh 12.1 Introduction 342 12.2 Pluripotent Stem Cells 342 12.3 Substrates for Maintenance of Self-renewal and Pluripotency of PSCs 344 12.3.1 Cellular Substrates 344 12.3.2 Acellular Substrates 345 12.3.2.1 Biological Matrices 345 12.3.2.2 ECM Components 348 12.3.2.3 Decellularized Matrices 350 12.3.2.4 Cell Adhesion Molecules 351 12.3.2.5 Synthetic Substrates 352 12.4 Substrates for Promoting Differentiation of PSCs 355 12.4.1 Cellular Substrates 355 12.4.2 Acellular Substrates 356 12.4.2.1 Biological Matrices 356 12.4.2.2 ECM Components 358 12.4.2.3 Decellularized Matrices 362 12.4.2.4 Cell Adhesion Molecules 363 12.4.2.5 Synthetic Substrates 363 12.5 Conclusions 366 Acknowledgments 367 References 367 13 Silk as a Natural Biopolymer for Tissue Engineering 379 Ayşe Ak Can and Gamze Bölükbaşi Ateş 13.1 Introduction 380 13.2 SF as a Biomaterial 383 13.2.1 Fibroin Hydrogels and Sponges 384 13.2.2 Fibroin Films and Membranes 386 13.2.3 Nonwoven and Woven Silk Scaffolds 386 13.2.4 Silk Fibroin as a Bioactive Molecule Delivery 386 13.3 Biomedical Applications of Silk-based Biomaterials 387 13.3.1 Bone Tissue Engineering 387 13.3.2 Cartilage Tissue Engineering 389 13.3.3 Ligament and Tendon Tissue Engineering 391 13.3.4 Cardiovascular Tissue Engineering 391 13.3.5 Skin Tissue Engineering 393 13.3.6 Other Applications of Silk Fibroin 393 13.4 Conclusion and Future Directions 393 References 394 14 Applications of Biopolymer-based, Surface-modified Devices in Transplant Medicine and Tissue Engineering 399 Ashim Malhotra, Gulnaz Javan and Shivani Soni 14.1 Introduction to Cardiovascular Disease 400 14.2 Need Assessment for Biopolymer-based Devices in Cardiovascular Therapeutics 400 14.3 Emergence of Surface Modification Applications in Cardiovascular Sciences: A Historical Perspective 401 14.4 Nitric Oxide Producing Biosurface Modification 403 14.5 Surface Modification by Extracellular Matrix Protein Adherence 404 14.6 The Role of Surface Modification in the Construction of Cardiac Prostheses 405 14.7 Biopolymer-based Surface Modification of Materials Used in Bone Reconstruction 406 14.8 The Use of Biopolymers in Nanotechnology 409 14.8.1 Protein Nanoparticles 410 14.8.1.1 Albumin-based Nanoparticles and Surface Modification 411 14.8.1.2 Collagen-based Nanoparticles and Surface Modification 412 14.8.1.3 Gelatin-based Nanoparticle Systems 413 14.8.2 Polysaccharide-based Nanoparticle Systems 413 14.8.2.1 The Use of Alginate for Surface Modifications 413 14.8.2.2 The Use of Chitosan-based Nanoparticles and Chitosan-based Surface Modification 414 14.8.2.3 The Use of Chitin-based Nanoparticles and Chitin-based Surface Modification 416 14.8.2.4 The Use of Cellulose-based Nanoparticles and Cellulose-based Surface Modification 417 References 418 15 Stem Cell Behavior on Microenvironment Mimicked Surfaces 423 M. Özgen Öztürk Öncel and Bora Garipcan 15.1 Introduction 424 15.2 Stem Cells 425 15.2.1 Definition and Types 425 15.2.1.1 Embryonic Stem Cells 426 15.2.1.2 Adult Stem Cells 426 15.2.1.3 Reprogramming and Induced Pluripotent Stem Cells 427 15.2.2 Stem Cell Niche 427 15.3 Stem Cells: Microenvironment Interactions 428 15.3.1 Extracellular Matrix 429 15.3.2 Signaling Factors 429 15.3.3 Physicochemical Composition 430 15.3.4 Mechanical Properties 430 15.3.5 Cell–Cell Interactions 431 15.4 Biomaterials as Stem Cell Microenvironments 431 15.4.1 Surface Chemistry 431 15.4.2 Surface Hydrophilicity and Hydrophobicity 434 15.4.3 Substrate Stiffness 435 15.4.4 Surface Topography 435 15.5 Biomimicked and Bioinspired Approaches 436 15.5.1 Bone Tissue Regeneration 439 15.5.2 Cartilage Tissue Regeneration 440 15.5.3 Cardiac Tissue Regeneration 441 15.6 Conclusion 442 References 442
£176.36
Taylor & Francis Ltd Aging
Book SynopsisThis book is a detailed and comprehensive synthesis of the scientific study of aging. Dozens of contributions from leading scholars review various theories of aging, and molecular, cellular, biochemical and microbial aspects of aging, among just a few of the topics included. Authoritative, wide ranging and thorough, this book will act as a source for experimental design, a comprehensive description of age related diseases, and provide information of the latest molecular theories underlying their causes. Additionally, it will target industries involved in developing anti-aging drugs, post-graduate medical students, and university libraries.Table of ContentsWhat is aging. Biological theories of aging. Free radical theory. Mitochondrial dysfunction theory. Error catastrophe theory. Evolutionary theory of aging. Fading electricity theory. Quasi-program theory for aging. Multiple hormone deficiency theory of aging. Oxidation–inflammation theory of aging. Reproductive-cell cycle theory of aging. Telomeres and telomerase theory of aging. Markov Models of aging. Immunological theory of aging. Social theory of aging. Genetic program theory of aging. Psychological theory of aging. Healthy living and its effects on aging. Longevity in aging. Impact of climate on aging. Centenarians in Okinawa. Genetics of the population. Caloric restriction, healthy diet. Staying young at heart. Genetics of aging and longevity. Metabolic programming, exercise, caloric restriction and aging. Premature aging. Progeria and Werner’s syndrome. Stem cell aging in progeria. Oxidative and nitrosative stress and accelerative aging. Progeria: Genetics, and lamin disorder. Molecular aspect of aging. Hematopoietic stem cells aging. Immune system and aging. Sirtuins in aging and age related diseases. AMPK function in aging. Aging mechanisms and lipid changes. Biochemical aspects of aging. GABA(A) receptors in aging. The ubiquitin-proteasome system and aging. Autophagy and aging. Oxidation of potassium channels and aging. Proteins and lipids in aging. Spatial protein quality control and aging. Klotho protein and aging. Glutathione as antioxidant. Neuropeptide Y as an anti-aging agent. Adipokines and aging. Lamins and aging. Sestrins and aging. HDL metabolism and aging. Growth hormone and aging. DNA damage, repair and aging. DNA damage, repair and neurodegenerative diseases. yH2AX molecular marker for DNA damage and aging. DNA repair in organelles and aging. Transcriptional blocking and DNA damage in aging. Microbiota and aging. Neuroscience of aging. Thermal stress in human and aging. Age related diseases. Ischemic heart disease and aging. Endothelial and vascular aging. The aging heart. Age related eye disease. Age related inflammation and osteoarthritis. Aging and musculoskeletal health. Cancer and aging. Sexual health in aging. Sleep disturbance in aging. Anxiety in aging. Female sexuality and aging. Aging and circadian rhythm. Metabolic syndrome and aging. Environmental toxicants and aging. Stem cell and aging. Non-human models for aging studies. Aging modulation in multiple species. Mouse models. Murine genetic models. C. elegans model. S. cerevisiae model. Drosophila model. Pig model. Mammalian hibernation and aging. Neobiosis as a predecessor of mammalian hibernation. Chemosensory and chemosensory systems and aging. Molecular biology of freezing tolerance. Freezing susceptibility in arthropods. Proteomic approach to study mechanisms of aging. Therapeutic approach for facial aging. Integrative medicine research on aging population. Meditation and aging. Life after death: Fact or fiction.
£147.25
Taylor & Francis Ltd Stem Cell Research
Book SynopsisStem Cell Research takes a multi-disciplinary approach to the topic of human embryonic stem cell research, starting with the breakthrough discovery up through the present day controversy. The book invites the reader to join the conversation by providing a well balanced approach to many of the issues surrounding the development of this controversial scientific field. It includes the thoughts and experiences of scientists, journalists and ethicists as it tried to approach the topic through a variety of different academic disciplines.The book will help the non-scientist understand the biology, research regulations and funding; and simultaneously it will help the scientist better comprehend the full spectrum of ethical, religious, and policy debates.Table of ContentsStem Cells and the Research Controversy. The Biology of Stem Cells. The Role of the Media. Regulation of the Research: Who Cares or Should Care. Who Pays for the Research: Public and Private Funding. The ethics of Stem Cell Research. Conclusion.
£42.74
Pearson Education Limited Beckers World of the Cell Global Edition
Book SynopsisJEFFHARDIN received his Ph.D. in Biophysics from the University ofCaliforniaBerkeley. He is the Raymond E. Keller Professor and Chair of theDepartment of Integrative Biology at the University of WisconsinMadison, wherehe has been since 1991. For 18 years he was Faculty Director of the BiologyCore Curriculum, a four-semester honors biology sequence for undergraduates atWisconsin known for its teaching innovations. Jeff 's research focuses on howcells migrate and adhere to one another during early embryonic development.Jeff 's teaching is enhanced by his extensive use of digital microscopy and hisweb-based teaching materials, which are used on many campuses in the UnitedStates and in other countries. Jeff was a founding member of the UW TeachingAcademy, and has received several teaching awards, including a Lily TeachingFellowship, a National Science Foundation Young Investigator Award, and aChancellor's Distinguished Teaching Award. JAMES P.LODOLCE earned his Ph.D. in ImmunologTable of Contents1. A Preview of Cell Biology2. The Chemistry of the Cell3. The Macromolecules of the Cell4. Cells and Organelles5. Bioenergetics: The Flow of Energy in the Cell6. Enzymes: The Catalysts of Life7. Membranes: Their Structure, Function, and Chemistry8. Transport Across Membranes: Overcoming the Permeability Barrier9. Chemotrophic Energy Metabolism: Glycolysis and Fermentation10. Chemotrophic Energy Metabolism: Aerobic Respiration11. Phototrophic Energy Metabolism: Photosynthesis12. The Endomembrane System and Protein Sorting 13. Cytoskeletal Systems14. Cellular Movement: Motility and Contractility15. Beyond the Cell: Cell Adhesions, Cell Junctions, and Extracellular Structures16. The Structural Basis of Cellular Information: DNA, Chromosomes, and theNucleus17. DNA Replication, Repair, and Recombination18. Gene Expression: I. Transcription19. Gene Expression: II. The Genetic Code and Protein Synthesis20. The Regulation of Gene Expression21. Molecular Biology Techniques for Cell Biology22. Signal Transduction Mechanisms: I. Electrical and Synaptic Signaling inNeurons23. Signal Transduction Mechanisms: II. Messengers and Receptors24. The Cell Cycle and Mitosis25. Sexual Reproduction, Meiosis, and Genetic Recombination26. Cancer CellsAppendix: Visualizing Cells and Molecules
£61.74
Macmillan Learning Molecular Biology
Book Synopsis
£75.04
W. H. Freeman Molecular Cell Biology 842581
Book Synopsis
£325.41
W. W. Norton & Company Essential Cell Biology
Book Synopsis
£111.75
Springer-Verlag New York Inc. Bioelectromagnetics Current Concepts
Book SynopsisProceedings of the NATO Advanced Research Workshop on The Mechanisms of the Biological Effect on Extra High Power Pulses (EHPP), Yerevan, Armenia 3 - 5 March 2005Table of ContentsPreface.Acknowledgements. Chapter I. Mechanisms of EMF interactions with biological systems. Thermal vs. nonthermal Mechanisms of Interactions between Electromagnetic Fields and Biological Systems; M. Markov.- The Mechanisms Paradox; K. Foster.- Cell Aqua Medium as a Primary Target for the Effecy of Electronmagnetic Fields; S.Ayrapetyan.- The Effect of ELF EMF-Pretreated Distillated Water on Barley seed Hydration and Germination Potential; A. Amyan and S. Ayrapetyan.- Intracellular Calcium Signaling Basic Mechanisms and Possible Alterations; P. Kostyuk and E. Lukyanetz.-Suppression of Synaptic Transmission in Hippocampus by extremely-high-power Microwave Pulses Synchronized with Neuronal Excitation; J. Doyle et al.-The in vitro Assessment of Potential Genotoxicity of High Power Microwave Pulses; N. Chemeris at al.- Unconventional Approach to Biological Effects of EMF; B. Sernelius.- The Effect of Iron Ions and weak static or low Frequency(50 HZ) Magnetic Fields on Lymphocytes: free Radical Processes; J. Jajte and M. Zmyslony.-Collagen as a Target for Electromagnetic Fields. Effects of 910-mhz on Rat Brain; M. Tzaphlidou and E. Fotiou.-Animal Studies on the Effects of ELF and Static EMF; N.Seyhan et al.- Chapter II. EMF Therapy. Interactions Between Electromagnetic Fields and Immune System: Possible Mechanism for Pain Control; M.Markov et al.- Electromagnetic Field Therapy: A Role for Water?;C. Hazlewood et al.- Physiological Mechanisms Underlying Millimeter Wave Therapy; M. Ziskin.-Anti-Inflammatory Effects of low-intensity Millimeter Wave Radiation; A.Gapeyev et al.- Study of the Secretion of Melatonin and Stress Hormones in Operators from Broadcasting and TV-Stations Exposed to Radiofrequency (RF) Electromagnetic Radiation (EMR) ; M. Israel et al.-The Combining Effect of the ferrocene Compounds with electro magnetic Field ; A. F. Badawi and A.A.Hafiz.-Chapter III. EMF Dosimetry. High-Frequency Device for the Measurement of the Specific Absorbed Rate by the Biotissues of High Intensity; R. Simonyan et al.-Physical Aspects of Pulsed Microwave Absorption in Tissue; P.Šístek.- Exposure Metrics of Magnetic Fields related to Power Lines and electric Applicances T. Saito et al.-Chapter IV. Epidemiology and policy Science, Uncertainty and Policy for Power and Mobile Frequency EMF; L. Kheifets.-Risk Evaluation of Potential Environmental Hazards from low energy electromagnetic Field Exposure using sensitive In Vitro Methods; F. Adlkofer.-Can the Radiation from Cellular Phones have important Effects on the Forces between Biological-Tissue-Components?; B.E.Sernelius.-Exposure to non-ionizing radiation of personnel in Physiotherapy; M.Israel and P. Tschobanoff.- Chapter V. Posters. Changes of the Magnitude of Arteriolar Vasomotion during and after ELF-EMF Exposure in Vivo L.Traikov et al.-The Effects of SMF, EHPP and Hydrogen Peroxide on the Development of Yeasts; N. Baghdasaryan and S.Ayrapetyan.- Inhibition of Melatonin Synthesis in Human Peripheral Blood Lymphocytes by EMF: A Mechanism of Interaction? ; R.Coghill and R. Baghurst.- A Study of Melatonin in Plant Tissues and its Dietary and Health Implications; R. Baghurst and R.Coghill.-Effect of High Dilution Quinones on 02 uptake by peripheral Blood Lymphocytes: A Polarographic Study; C. Conners and R. Coghill.-Internal originators of Functions Fluctuation in Multi-Celled Organisms; R.D. Grygoryan and P.N. Lissov.-List of speakers. List of participants. Official Photograph. Index
£170.99
John Wiley and Sons Ltd Cellular Physiology of Nerve and Muscle
Book SynopsisCellular Physiology of Nerve and Muscle, Fourth Edition offers a state of the art introduction to the basic physical, electrical and chemical principles central to the function of nerve and muscle cells. The text begins with an overview of the origin of electrical membrane potential, then clearly illustrates the cellular physiology of nerve cells and muscle cells. Throughout, this new edition simplifies difficult concepts with accessible models and straightforward descriptions of experimental results. An all-new introduction to electrical signaling in the nervous system. Expanded coverage of synaptic transmission and synaptic plasticity. A quantitative overview of the electrical properties of cells. New detailed illustrations. Table of ContentsPart I: Origin of Electrical Membrane Potential. 1. Introduction to Electrical Signaling in the Nervous System. The Patellar Reflex as a Model for Neural Function. The Cellular Organization of Neurons. Electrical Signals in Neurons. Transmission between Neurons. 2. Composition of Intracellular and Extracellular Fluids. Intracellular and Extracellular Fluids. The Structure of the Plasma Membrane. Summary. 3. Maintenance of Cell Volume. Molarity, Molality, and Diffusion of Water. Osmotic Balance and Cell Volume. Answers to the Problem of Osmotic Balance. Tonicity. Time-Course of Volume Changes. Summary. 4. Membrane Potential: Ionic Equilibrium. Diffusion Potential. Equilibrium Potential. The Nernst Equation. The Principle of Electrical Neutrality. The Cell Membrane as an Electrical Capacitor. Incorporating Osmotic Balance. Donnan Equilibrium. A Model Cell That Looks Like a Real Animal Cell. The Sodium Pump. Summary. 5. Membrane Potential: Ionic Steady State. Equilibrium Potentials for Sodium, Potassium, and Chloride. Ion Channels in the Plasma Membrane. Membrane Potential and Ionic Permeability. The Goldman Equation. Ionic Steady State. The Chloride Pump. Electrical Current and the Movement of Ions Across Membranes. Factors Affecting Ion Current Across a Cell Membrane. Membrane Permeability vs. Membrane Conductance. Behavior of Single Ion Channels. Summary. Part II: Cellular Physiology of Nerve Cells. 6. Generation of Nerve Action Potential. The Action Potential. Ionic Permeability and Membrane Potential. Measuring the Long-Distance Signal in Neurons. Characteristics of the Action Potential. Initiation and Propagation of Action Potentials. Changes in Relative Sodium Permeability During an Action Potential. Voltage-Dependent Sodium Channels of the Neuron Membrane. Repolarization. The Refractory Period. Propagation of an Action Potential Along a Nerve Fiber. Factors Affecting the Speed of Action Potential Propagation. Molecular Properties of the Voltage-Sensitive Sodium Channel. Molecular Properties of Voltage-Dependent Potassium Channels. Calcium-Dependent Action Potentials. Summary. 7. The Action Potential: Voltage Clamp Experiments. The Voltage Clamp. Measuring Changes in Membrane Ionic Conductance Using the Voltage Clamp. The Squid Giant Axon. Ionic Currents Across an Axon Membrane Under Voltage Clamp. The Gated Ion Channel Model. Membrane Potential and Peak Ionic Conductance. Kinetics of the Change in Ionic Conductance Following a Step Depolarization. Sodium Inactivation. The Temporal Behavior of Sodium and Potassium Conductance. Gating Currents. Summary. 8. Synaptic Transmission at the Neuromuscular Junction. Chemical and Electrical Synapses. The Neuromuscular Junction as a Model Chemical Synapse. Transmission at a Chemical Synapse. Presynaptic Action Potential and Acetylcholine Release. Effect of ACh on the Muscle Cell. Neurotransmitter Release. The Vesicle Hypothesis of Quantal Transmitter Release. Mechanism of Vesicle Fusion. Recycling of Vesicle Membrane. Inactivation of Released Acetylcholine. Recording the Electrical Current Flowing Through a Single Acetylcholine-Activated Ion Channel. Molecular Properties of the Acetylcholine-Activated Channel. Summary. 9. Synaptic Transmission in the Central Nervous System. Excitatory and Inhibitory Synapses. Excitatory Synaptic Transmission Between Neurons. Temporal and Spatial Summation of Synaptic Potentials. Some Possible Excitatory Neurotransmitters. Conductance-Decrease E.P.S.P.'s. Inhibitory Synaptic Transmission. The Synapse Between Sensory Neurons and Antagonist Neurons in the Patellar Reflex. Characteristics of Inhibitory Synaptic Transmission. Mechanism of Inhibition in the Postsynaptic Membrane. Some Possible Inhibitory Neurotransmitters. The Family of Neurotransmitter-Gated Ion Channels. Neuronal Integration. Indirect Actions of Neurotransmitters. Presynaptic Inhibition and Factilitation. Synaptic Plasticity. Short-Term Changes in Synaptic Strength. Long-Term Changes in Synaptic Strength. Summary. Part III: Cellular Physiology of Muscle Cells. 10. Excitation-Contraction Coupling in Skeletal Muscle. The Three Types of Muscle. Structure of Skeletal Muscle. Changes in Striation Pattern on Contraction. Molecular Composition of Filaments. Interaction Between Myosin and Actin. Regulation of Contraction. The Sarcoplasmic Reticulum. The Transverse Tubule System. Summary. 11. Neural Control of Muscle Contraction. The Motor Unit. The Mechanics of Contraction. The Relationship Between Isometric Tension and Muscle Length. Control of Muscle Tension by the Nervous System. Recruitment of Motor Neurons. Fast and Slow Muscle Fibers. Temporal Summation of Contractions Within a Single Motor Unit. Asynchronous Activation of Motor Units During Maintained Contraction. Summary. 12. Cardiac Muscle: The Autonomic Nervous System. Autonomic Control of the Heart. The Pattern of Cardiac Contraction. Coordination of Contraction Across Cardiac Muscle Fibers. Generation of Rhythmic Contractions. The Cardiac Action Potential. The Pacemaker Potential. Actions of Acetylcholine and Norepinephrine on Cardiac Muscle Cells. Summary. Appendix A: Derivation of the Nernst Equation. Appendix B: Derivation of the Goldman Equation. Appendix C: Electrical Properties of Cells. Suggested Readings
£59.36
John Wiley and Sons Ltd Annual Plant Reviews Membrane Transport in Plants
Book SynopsisAnnual Plant Reviews, Volume 15 This volume addresses some of the most important and hotly-pursued topics in the field of plant membrane transport. The first two chapters consider membrane transport analysis, emphasizing concepts, techniques and tools for electrophysiology. Chapters 3-8 divide along boundaries of pumps, coupled transporters and channels; the addition of a chapter on water channels highlights this rapidly expanding and, until recently, highly controversial topic. Chapters 9 and 10 deal with issues of Ca2+ and H+ signalling, and of membrane traffic that increasingly attracts the attention of researchers in plant development. Finally, chapters 11 and 12 take a post-genomic look at the problems of understanding the integration of transport mechanisms and its relevance to inorganic nutrition and phytoremediation. An overriding theme throughout is the extent to which the research on membrane transport now informs the fields of plaTable of Contents1. Concepts and techniques in plant membrane physiology. Michael R. Blatt, Laboratory of Plant Physiology and Biophysics, University of Glasgow, UK. 2. Electrophysiology equipment and software. Adrian Hills and Vadim Volkov, Laboratory of Plant Physiology and Biophysics, University of Glasgow, UK. 3. Structure, function and regulation of primary H+ and Ca2+ pumps. Rosa L. López-Marqués, Morten Schiøtt, Mia Kyed Jakobsen and Michael G. Palmgren, Department of Plant Biology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark. 4. Ion-coupled transport of inorganic solutes. Malcolm J. Hawkesford and Anthony J. Miller, Crop Performance and Improvement Division, Rothamsted Research, Harpenden, UK. 5. Functional analysis of proton-coupled sucrose transport. Daniel R. Bush, USDA-ARS and Plant Biology, University of Illinois, Urbana, USA. 6. Voltage-gated ion channels. Ingo Dreyer, Bernd Müller-Röber and Barbara Köhler, Universität Potsdam, Institut für Biochemie und Biologie, Golm, Germany. 7. Ligand-gated ion channels. Frans Maathius, Biology Department, University of York, UK. 8. Aquaporins in plants. Clare Vander Willigen, Lionel Verdoucq, Yann Boursiac and Christophe Maurel, Department of Molecular and Cellular Biology, University of Cape Town, South Africa. 9. Ca2+ and pH as integrating signals in transport control. Tatiana N. Bibikova, Sarah M. Assmann and Simon Gilroy, Biology Department, Penn. State University, Pennsylvania, USA. 10. Vesicle traffic and plasma membrane transport. Annette C Hurst, Gerhard Thiel and Ulrike Homann, Botanisches Institut, TU-Darmstadt, Darmstadt, Germany. 11. Potassium nutrition and salt stress. Anna Amtmann, Patrick Armengaud and Vadim Volkov, Laboratory of Plant Physiology and Biophysics, University of Glasgow, UK. 12. Membrane transport and soil bioremediation. Susan Rosser and Peter Dominy, Plant Sciences, IBLS, University of Glasgow, UK. References . Index
£242.06
John Wiley and Sons Ltd Annual Plant Reviews Intercellular Communication
Book SynopsisAnnual Plant Reviews, Volume 16 Intercellular communication in plants plays a vital role in the co-ordination of processes leading to the formation of a functional organism.Table of Contents1. Auxin as an intercellular signal. Jiri Friml and Justyna Wisniewska, Department of Developmental Genetics, University of Tübingen, Germany. 2. Peptides as signals. Yiji Xia, Donald Danforth Plant Science Center, St Louis, Missouri, USA. 3. RNA as a signalling molecule. Patrice Dunoyer and Olivier Voinnet, Institut de Biologie Moléculaire des Plantes, CNRS, Strasbourg, France. 4. The plant extracellular matrix and signalling. Andrew Fleming, Department of Animal and Plant Sciences, University of Sheffield, UK. 5. Plasmodesmata – gateways for intercellular communication in plants. Trudie Gillespie and Karl Oparka, Scottish Crop Research Institute, Dundee, UK. 6. Lessons from the vegetative shoot apex. John Golz, School of Biological Sciences, University of Victoria, Australia. 7. Intercellular communication during floral initiation and development. George Coupland, Max Planck Institute for Plant Breeding, Cologne, Germany. 8. Lessons from the root apex. Martin Bonke, Sari Tähtiharju and Ykä Helariutta, Institute of Biotechnology, University of Helsinki, Finland. 9. Lessons from leaf epidermal patterning in plants. Bhylahalli Purushottam and Martin Hülskamp, Botanical Institute, University of Cologne, Germany. 10. Lessons on signalling in plant self-incompatibility systems. Andrew G. McCubbin, School of Biological Sciences, Washington State University, Pullmann, USA. References. Index
£218.66
John Wiley and Sons Ltd Annual Plant Reviews Plasmodesmata
Book SynopsisAnnual Plant Reviews, Volume 18 Since their discovery over 100 years ago, plasmodesmata have been the focus of intense investigation. Plasmodesmata are unique to plants and form an intercellular continuum for the transport of solutes, signals and ribonucleoprotein complexes.Table of ContentsContributors. Preface. 1. Plasmodesmal structure and development. Alison G. Roberts, Scottish Crop Research Institute, Dundee, UK. 2. Evolution of plasmodesmata. John A. Raven, Division of Environmental and Applied Biology, University of Dundee, UK. 3. Plasmodesmata: protein transport signals and receptors. Friedrich Kragler, Vienna Biocenter, Austria. 4. Comparative structures of specialised monocotyledonous leaf blade plasmodesmata. C. E. J. Botha, Botany Department, Rhodes University, Grahamstown, South Africa, R. H. M. Cross, Electron Microscopy Unit, Rhodes University, Grahamstown, South Africa and L. Liu, Linyi University, Linyi, China. 5. Plasmodesmata and plant morphogenesis. Ken Kobayashi, Insoon Kim, Euna Cho and Patricia Zambryski, Department of Plant and Microbial Biology, University of California, Berkeley, USA. 6. Transcription factor movement through plasmodesmata. David Jackson, Cold Spring Harbor Laboratory, New York, USA. 7. Role of plasmodesmata in solute loading and unloading. Alexander Schulz, Department of Plant Biology, The Royal Veterinary and Agricultural University, Copenhagen, Denmark. 8. Plasmodesmata and the phloem: conduits for local and long-distance signaling. Robert L. Gilbertson, Maria R. Rojas, and William J. Lucas, Department of Plant Pathology, University of California, Davis, USA. 9. Movement of viruses to and through plasmodesmata. Richard S. Nelson, Samuel Roberts Noble Foundation, Ardmore, Oklahoma, USA. 10. Systemic RNA silencing. Manfred Heinlein, Institute of Botany, University of Basel, Switzerland. 11. Techniques for imaging intercellular transport. Karl Oparka and Petra Boevink, Unit of Cell Biology, Scottish Crop Research Institute, Dundee, UK. 12. Electrical signalling via plasmodesmata. Aart J. E. van Bel and Katrina Ehlers, Institute of General Botany, Justus-Liebig University, Giessen, Germany. 13. Regulation of plasmodesmal conductance. Terena L. Holdaway-Clarke, School of Biological Sciences, University of Sydney, New South Wales, Australia. Index
£205.16
John Wiley and Sons Ltd Annual Plant Reviews Cell Cycle Control and Plant
Book SynopsisThe cell cycle in plants consists of an ordered set of events, including DNA replication and mitosis, that culminates in cell division. As cell division is a fundamental part of a plant's existence and the basis for tissue repair, development and growth, a full understanding of all aspects of this process is of pivotal importance. Cell Cycle Control and Plant Development commences with an introductory chapter and is broadly divided into two parts. Part 1 details the basic cell machinery, with chapters covering cyclin-dependent kinases (CDKs), cyclins, CDK inhibitors, proteolysis, CDK phosphorylation, and E2F/DP transcription factors. Part 2, which describes the cell cycle and plant development, covers cell cycle activation, cell cycle control during leaf development, endoreduplication, the cell cycle and trichome, fruit and endosperm development, the hormonal control of cell division and environmental stress, and cell cycle exit. The editor of this important bookTrade Review"Excellent new volume ... .An essential purchase for research teams ... .All libraries ... should have copies of this essential and timely volume." Biotechnology, Agronomy, Society and Environment “Welcome and timely ... .Much of the information was relatively new to me; an enjoyable read … .Full of excellent information, carefully and thoughtfully presented.” Annals of BotanyTable of Contents1. The growing family of plant cyclin-dependent kinases with multiple functions in cellular and developmental regulation. 2. The plant cyclins. 3. CDK inhibitors. 4. The UPS: an engine that drives the cell cycle. 5. CDK phosphorylation. 6. E2F-DP transcription factors. 7. Function of the retinoblastoma-related protein in plants. 8. Auxin fuels the cell cycle engine during lateral root initiation. 9. Cell cycle control during leaf development. 10. Physiological relevance and molecular control of the endocycle in plants. 11. Insights into the endocycle from trichome development. 12. Cell cycle control and fruit development. 13. Cell cycle and endosperm development. 14. Hormonal regulation of cell cycle progression and its role in development. 15. Cell cycle and environmental stresses
£172.76
Taylor & Francis Inc Copper Amine Oxidases
Book SynopsisAlthough the amount of research on copper amine oxidases has grown rapidly and substantially in the past decade, the field unfortunately suffers from lack of cohesion and significant confusion surrounds aspects as simple as confirmation of enzyme identities. This book describes the structure of the enzymes, the role of copper, and of the unusual cofactor 6-hydroxydopa quinine derived from a posttranslational modification of a tyrosine residue. It also covers the differences of between AOs from bacteria, plants, and mammals. Finally, the text examines the importance of this ubiquitous class of enzymes in physiology and in metabolism of biogenic amines.Table of ContentsHistory. Nomenclature and Potential Functions of Copper Amine Oxidases. Cofactors of Amine Oxidases: Copper Ion and Its Substitution and the 2, 4, 5-Trihydroxylphenylalanine Quinone. Copper Amine Oxidases from Plants. Soluble Copper Amine Oxidases from Mammals. Membrane-Bound Copper Amine Oxidases. Copper Amine Oxidase Genes. Mechanism of TPQ Biogenesis in Prokaryotic Copper Amine Oxidase. Copper Amine Oxidase Crystal Structures. Plasma Amine Oxidases in Various Clinical Conditions and in Apoptosis. Copper Amine Oxidases in Intestine Diseases. Copper Amine Oxidases in Adipose Tissue-Related Disorders. Copper Amine Oxidases in Adhesion Molecules in Renal Pathology and in Alzheimer’s Disease and VAP-1 in Leukocyte Migration. Inhibitors of Copper Amine Oxidases: Past, Present, and Future. Pharmacological Applications of Copper Amine Oxidases. Copper Amine Oxidases as Antioxidant and Cardioprotective Agents. Biotechnological Aspects of Copper Amine Oxidases. Concluding Remarks. References.
£180.50
University of Toronto Press Dreams and Due Diligence
Book SynopsisDreams and Due Diligence vividly chronicles the work of two researchers who made medical history two men who possessed exactly the right complementary talents to achieve greatness and win nearly every award available in medical research.Table of ContentsContents Introduction Part I: Discovery 1. On a Sunday in 1960 2. After the A-Bomb, before the Beatles 3. The Impossible partnership Part II: Development 4. A bunch of kids having a good time 5. The progeny Part III: Today and Tomorrow 6. Ethics, hope and hype 7. The evil twin: the cancer stem cell 8. The beneficiary 9. The future 10. Little fame, no Nobel Conclusion
£26.99
Time Warner Trade Publishing The Breakthrough Immunotherapy and the Race to
Book SynopsisFollow along as this New York Times bestselling author details the astonishing scientific discovery of the code to unleashing the human immune system to fight in this captivating and heartbreaking book (The Wall Street Journal).For decades, scientists have puzzled over one of medicine''s most confounding mysteries: Why doesn''t our immune system recognize and fight cancer the way it does other diseases, like the common cold?As it turns out, the answer to that question can be traced to a series of tricks that cancer has developed to turn off normal immune responses -- tricks that scientists have only recently discovered and learned to defeat. The result is what many are calling cancer''s penicillin moment, a revolutionary discovery in our understanding of cancer and how to beat it.In The Breakthrough, New York Times bestselling author of The Good Nurse Charles Graeber guides readers through the revolutionary scientific re
£15.29
Springer-Verlag New York Inc. Cell and Muscle Motility
Book SynopsisMotility is a fundamental property of living systems, from the cytoplasmic streaming of unicellular organisms to the most highly differentiated and de veloped contractile system of higher organisms, striated muscle. Although of motility have a long and in scientific investigations into the mechanisms teresting history, the knowledge of molecular processes, especially in the area of regulation of control of motility, has been developing at an ever more rapid pace with the utilization of multidisciplinary approaches from physiology, cell biology, genetics, biochemistry, pharmacology, and biophysics. In Volume 3, Cell and Muscle Motility continues the same philosophy as that of the preceding volumes. The essays are meant to focus on topics of current interest, to be critical rather than exhaustive, and to indicate the current trends of research efforts. The series is intended to fosTable of Contents1 Intermediate Filaments in Striated Muscle: A Review of Structural Studies in Embryonic and Adult Skeletal and Cardiac Muscle.- 2 Biochemistry and Structure of Mammalian Neurofilaments.- 3 Organization of Contractile Fibers in Smooth Muscle.- 4 Regulation of Muscle Contraction.- 5 The Membrane System and Cytoskeletal Elements of Mammalian Myocardial Cells.- 6 Control of Gene Expression in Muscle Development.- 7 Cloning of Contractile Protein.- 8 Role of Microtubules and Centrioles in Growth Regulation of Mammalian Cells.
£40.49
Taylor & Francis Inc Cytochrome P450 2D6
Book SynopsisCytochromes are proteins that catalyze electron transfer reactions of well-known metabolic pathways and are classified in various superfamilies. The CYP, or P450, superfamily accounts for 90% of the oxidative metabolism of clinical drugs. One member of this superfamily, P450 2D6 (or CYP2D6), singlehandedly metabolizes about 25% of all medications in the human liver. Cytochrome P450 2D6: Structure, Function, Regulation, and Polymorphism reviews the current knowledge of CYP2D6 as well as the maturing body of evidence indicating its significance to clinical and pharmacological researchers and practitioners.This book focuses on the critical role CYP2D6 plays in the human liver. It examines the genetic, epigenetic, physiological, pathological, and structural factors of the gene that govern the highly variable metabolism of a number of drugs in clinical use. It highlights the impact of the functional roles of CYP2D6 on clinical practice and drug development and also dTable of ContentsIntroduction to Human Cytochrome P450 Superfamily. Mammalian CYP2D Members: A Comparison of Structure, Function, and Regulation. Substrates of Human CYP2D6. Inhibitors of Human CYP2D6. Regulation of Human CYP2D6. Structure and Function of Human CYP2D6. Clinical Pharmacogenomics of Human CYP2D6. General Discussion about Human CYP2D6.
£237.50
Taylor & Francis Inc Protocols for Cytogenetic Mapping of Arthropod
Book SynopsisArthropods are important to worldwide agriculture, food safety, human health, and energy production. Besides their practical significance, various species represent excellent model systems for biological investigations of evolution, development, physiology, reproduction, and social interaction. For these reasons, arthropod genomics is receiving increasing attention from researchers around the globe. Protocols for Cytogenetic Mapping of Arthropod Genomes is a collection of up-to-date, detailed protocols for physical chromosome mapping and their applications for studying genome organization and evolution in insects and ticks. This book brings together the expertise of cytogeneticists working on diverse groups of arthropods. Each chapter demonstrates approaches to tissue dissection, chromosome preparation, fluorescence in situ hybridization, and imaging. The book is a timely and complementary effort to the i5K initiative, which will obtain whole-genome sequences foTable of ContentsTephritid Fruit Flies. Hessian Flies. Tsetse Flies. Mosquitoes. Beetles. Silk Moths. Parasitoid Wasps. Bedbugs. Aphids. Spittlebugs. Grasshoppers. Ticks. Index.
£166.25
Taylor & Francis Inc Connective Tissue
Book SynopsisConnective tissue is a multicomponent, polyfunctional complex of cells and extracellular matrix that serves as a framework for all organs, combining to form a unified organism. It is a structure responsible for numerous vital functions such as tissueorgan integration, morphogenesis, homeostasis maintenance, biomechanical support, and more. The regeneration potential of connective tissue affects healing of damaged tissue and organs, while trauma, stress, and other factors that cause damage to connective tissue can lead to numerous disorders.Connective Tissue: Histophysiology, Biochemistry, Molecular Biology brings together crucial knowledge of mammalian connective tissue (including human) and its components, both cellular and noncellular, in one authoritative reference. The breadth and depth of information has fundamental scientific significance as well as applied relevance in clinical medicine. The first half of the book covers the structure, classification, biochemicTable of ContentsPeculiarities of Connective Tissue Histophysiology, Biochemistry, and Molecular Biology. Cellular Elements of Connective Tissue. Extracellular Matrix of Connective Tissue: Biochemistry and Histophysiology. Regulation of Connective Tissue Metabolic Functions. Biochemical and Molecular Biological Mechanisms and Manifestations of Connective Tissue Ageing. Bone – An Organ of the Support and Locomotor Apparatus Containing All Types of Connective Tissue. Cartilage – Cartilaginous Tissue: Structural, Biochemical and Molecular Biological Characteristics. Bone Tissue: The Structural-Functional, Biochemical and Molecular Biological Characteristics of Its Components. Biochemical Characteristics of Synovial Membrane and Synovia. Molecular Biological and Biochemical Regularities of Connective Tissue Structures Ontogenesis. References. List Abbreviations.
£199.50
Taylor & Francis Inc Cell Membrane Nanodomains
Book SynopsisCell Membrane Nanodomains: From Biochemistry to Nanoscopy describes recent advances in our understanding of membrane organization, with a particular focus on the cutting-edge imaging techniques that are making these new discoveries possible. With contributions from pioneers in the field, the book explores areas where the application of these novel techniques reveals new concepts in biology. It assembles a collection of works where the integration of membrane biology and microscopy emphasizes the interdisciplinary nature of this exciting field.Beginning with a broad description of membrane organization, including seminal work on lipid partitioning in model systems and the roles of proteins in membrane organization, the book examines how lipids and membrane compartmentalization can regulate protein function and signal transduction. It then focuses on recent advances in imaging techniques and tools that foster further advances in our understanding of signaling nTable of ContentsPart 1 Diffraction-limited microscopy. Part 2 Super resolution microscopy and advancements in probes and data analysis. Part 3 Protein and lipid nanodomains.
£175.75
Taylor & Francis Inc Liver Metabolism and Fatty Liver Disease
Book SynopsisNonalcoholic fatty liver disease is one of the most common liver diseases worldwide affecting patients from all ages, races, and ethnic backgrounds. It comprises a spectrum of hepatic pathology ranging from simple steatosis, in which there is an increase of fat accumulation in hepatocytes, to nonalcoholic steatohepatitis and cirrhosis. The significant prevalence of this diseasebetween 15 and 45 percent of the general populationmeans that it contributes to an increased burden of ill health both today and in the future.Liver Metabolism and Fatty Liver Disease addresses the current understanding of the development of nonalcoholic fatty liver disease as well as the clinical aspects of the disease by examining the current knowledge surrounding metabolism in the liver. The book discusses various topics including the involvement of oxidative stress, metabolic effects, and inflammation as well as the effect of nutrition on the development and progression of the disease.Table of ContentsIntroduction to Fatty Liver Disease and Consequences. Fatty Liver Disease and Consequences. Nutrition Role in Liver Protection and Damage.
£147.25
Springer New York Branching Processes in Biology
Trade Review“This book is the result … of a fruitful and long collaboration between a mathematician and a cell biologist. Capturing the best of both worlds, the book provides not only the biology and mathematical background for this topic, but also offers numerous examples which render it accessible to (post-graduate) students and researchers … . this book can be treated as an excellent textbook for a wide audience varying from students to lecturers.” (Irina Ioana Mohorianu, zbMATH 1312.92004, 2015)"This book treats the theory of several important types of branching processes and demonstrates their usefulness by many interesting and important applications. … Mathematical theory and biological applications are nicely interwoven. This text will be useful both to mathematicians (including graduate students) interested in relevant applications of stochastic processes in biology, as well as to mathematically oriented biologists working on the above mentioned topics." (R. Bürger, Monatshefte für Mathematik, Vol. 143 (1), 2004)"This is a significant book on applications of branching processes in biology, and it is highly recommended for those readers who are interested in the application and development of stochastic models, particularly those with interests in cellular and molecular biology." (Charles J. Mode, Siam Review, Vol. 45 (2), 2003)"This is a book written jointly by a mathematician and a cell biologist, who have collaborated on research in branching processes for more than a decade. In their own words, their monograph is intended for ‘mathematicians and statisticians who have had an introduction to stochastic processes but have forgotten much of their college biology, and for biologists who wish to collaborate with mathematicians and statisticians.’ They have largely succeeded in achieving their goal. The book can be strongly recommended to all students of branching processes; all libraries should have a copy." —ZENTRALBLATT MATH Table of ContentsMotivating Examples and Other Preliminaries.- Biological Background.- The Galton-Watson Process.- The Age-Dependent Process: Markov Case.- The Bellman-Harris Process.- Multitype Processes.- Branching Processes with Infinitely Many Types.- Genealogies of Branching Processes and their Applications.- References.
£61.74
Humana Press Inc. Organoids
Book SynopsisThis detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research.Table of Contents1. A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment Magdalena Sutcliffe and Madeline A. Lancaster 2. Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells Eva Tomaskovic-Crook and Jeremy M. Crook 3. Organoid Assay of Human Cancer Stem Cells Yohei Shimono, Piero Dalerba, Junko Mukohyama, Taichi Isobe, Darius Johnston, and Akira Suzuki 4. Construction of Thymus Organoids from Decellularized Thymus Scaffolds Asako Tajima, Isha Pradhan, Xuehui Geng, Massimo Trucco, and Yong Fan 5. Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres Robert E. Hynds, Colin R. Butler, Sam M. Janes, and Adam Giangreco 6. Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells Mahboobe Ghaedi and Laura E. Niklason 7. Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix Hiroko Hisha and Hiroo Ueno 8. Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension Valentina Benedetti, Valerio Brizi, and Christodoulos Xinaris 9. Efficient Culture of Intestinal Organoids with Blebbistatin Zhen Qi and Ye-Guang Chen 10. Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion Dustin J. Flanagan, Renate H.M. Schwab, Bang M. Tran, Toby J. Phesse, and Elizabeth Vincan 11. The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine Malgorzata Pierzchalska, Malgorzata Panek, Malgorzata Czyrnek, and Maja Grabacka 12. New Trends and Perspectives in the Function of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice Toshio Takahashi 13. Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System Jessica L. Forbester, Nicholas Hannan, Ludovic Vallier, and Gordon Dougan 14. Murine Colonic Organoid Culture System and Downstream Assay Applications Yang-Yi Fan, Laurie A. Davidson, and Robert S. Chapkin 15. Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions Giulia Nigro, Melissa Hanson, Cindy Fevre, Marc Lecuit, and Philippe J. Sansonetti 16. The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism Diana Li, Hongli Dong, and Alison B. Kohan 17. Oncogenic Transformation of Human-Derived Gastric Organoids Nina Bertaux-Skeirik, Jomaris Centeno, Jian Gao, Joel Gabre, and Yana Zavros 18. Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation Yun Chen, Chuan Li, Sheng-Hong Tseng, and Ya-Hui Tsai 19. Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections Winnie Y. Zou, Sarah E. Blutt, Sue E. Crawford, Khalil Ettayebi, Xi-Lei Zeng, Kapil Saxena, Sasirekha Ramani, Umesh Karandikar, Nicholas C. Zachos, and Mary K. Estes 20. Study Bacterial-Host Interactions Using Intestinal Organoids Yong-guo Zhang and Jun Sun 21. Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering Megan K. Eldred, Leila Muresan, and William A. Harris 22. Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo Kai Tong, Mahendra Wagle, and Su Guo 23. Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells Yoni Baert, Charlotte Rombaut, and Ellen Goossens 24. Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids Tiziana A.L. Brevini, Elena F.M. Manzoni, Sergio Ledda, and Fulvio Gandolfi 25. Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly Yuqing Zhu, Vahid Serpooshan, Sean Wu, Utkan Demirci, Pu Chen, and Sinan Güven 26. Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying Mozhdeh Imaninezhad, Era Jain, and Silviya Petrova Zustiak 27. Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues Soojung Hahn and Jongman Yoo 28. Drug Sensitivity Assays of Human Cancer Organoid Cultures Hayley E. Francies, Andrew Barthorpe, Anne McLaren-Douglas, William J. Barendt, and Mathew J. Garnett
£89.99
Taylor & Francis Inc Mechanisms Linking Aging Diseases and Biological
Book SynopsisThis book focuses on four of the hallmarks of aging: aspartic acid racemization, advanced glycation end products, telomere shortening and mitochondrial mutations; describing their role in aging and diseases; and their application to age-at-death estimation in forensic sciences in greater depth, displaying the interconnecting pathways among these processes. An additional chapter related to Epigenetics and its role in aging, diseases, and forensic age estimation is also included. This book is aimed at a broad audience: from students being introduced to aging, diseases, and forensic science research to scientists in biomedicine and forensics complementing their knowledge in their respective fields while also increasing their knowledge in other disciplines.Table of ContentsAspartic Acid Racemization. Introduction to Aspartic Acid Racemization. Aspartic Acid Racemization on Aging. Aspartic Acid Racemization and Aging in Cartilaginous Tissue. Racemization and isomerization of Aspartyl Residues in Amyloid Peptides Involved in the Development of Alzheimer´s Disease. Aspartic Acid Racemization: Applications to Forensic and Archaeological Age Estimation. Advanced Glycation Endproducts. Advanced Glycation Endproducts: An Introduction. Advanced Glycation and Aging. AGE-RAGE Axis in the Aging and Diabetic Heart: Prime Target for Adjunctive Therapeutic Intervention. Advanced Glycation Endproducts in Aging Skin. Advanced Glycation Endproducts and Neurological Diseases. Advanced Glycation Endproducts for Age at Death Estimation. Telomeres. Introduction to Telomere Biology. Role of Telomeres in Aging. Telomeres and Cardiovascular Disease: Facts, Controversies and Limitations. Telomeres in Cancer. Forensic Application of Telomere Shortening in Age-at-death Estimation. Mitochondrial DNA Mutations. Introduction to Mitochondria Biology. Mitochondrial DNA Mutations and Aging. Mitochondrial DNA Mutations and Mitochondrial Diseases. Reproductive Approaches to Prevent the Transmission of Mitochondrial Diseases. Mitochondrial DNA Mutations and Neurodegenerative Diseases. An Overview of mtDNA Analysis for Age-at-death Estimation in Forensic Sciences. Epigenetics. Epigenetics: Its Role in Aging, Diseases and Biological Age Estimation.
£147.25
Taylor & Francis Inc Regenerative Medicine Technology
Book SynopsisMiniaturization in the fields of chemistry and molecular biology has resulted in the lab-on-a-chip. Such systems are micro-fabricated devices capable of handling extremely small fluid volumes facilitating the scaling of single or multiple lab processes down to a microchip-sized format. The convergence of lab-on-a-chip technology with the field of cell biology facilitated the development of organ-on-a-chip systems. Such systems simulate the function of tissues and organs, having the potential to bypass some cell and animal testing methods. These technologies have generated high interest as applications for disease modeling and drug discovery. This book, edited by Drs. Sean Murphy and Anthony Atala, provides a comprehensive coverage of the technologies that have been used to develop organ-on-a-chip systems. Known leaders cover the basics to the most relevant and novel topics in the field, including micro-fabrication, 3D bio-printing, 3D cell culture techniques, biosensor designTable of ContentsIntroduction. Microfabrication and 3D Bioprinting. 3D Cell Culture/Tissue Spheroids. Biosensors and Microelectronics. Microfluidics. Big Data/Predictive Analysis. Lab-on-a-Chip. Lung-on-a-Chip. Liver-on-a-Chip. Heart-on-a-Chip. Skin-on-a-Chip. Kidney-on-a-Chip. Body-on-a-Chip. Liver Cancer-on-a-Chip. Lung Cancer-on-a-Chip. Breast Cancer-on-a-Chip. Cancer Metastasis-on-a-Chip. Cancer Microenvironment-on-a-Chip. Disease Modeling. Toxicology Testing. Detection of Bioweapon Pathogens. Personalized Medicine.
£175.75
