Scientific equipment, experiments and techniques Books

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Book SynopsisMedical imaging of cardiovascular disease has improved rapidly with faster multislice CT angiography. The ability to visualise 3D reconstructed vascular structures has given clinicians a much better appreciation of the anatomy and the disease process. This book provides an overview of the diagnostic applications of CT virtual intravascular endoscopy (VIE) in cardiovascular disease. It is intended to fill the gap by providing a systematic overview of the diagnostic applications of VIE in cardiovascular disease. This book serves as a comprehensive piece of literature that encompasses all aspects from the technical principles of VIE image generation to diagnostic value and potential pitfalls of VIE in cardiovascular imaging. The primary motion to write this book is to provide an overview of the CT VIE in cardiovascular disease with a focus on the clinical applications and diagnostic value of this unique visualisation tool. It is expected that readers, especially clinicians will gain a good understanding of the diagnostic value of CT VIE in the diagnosis of cardiovascular disease, so that their medical practice is enhanced with use of this exciting complementary visualisation tool.

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Book SynopsisBased on the mature development of microfabrication technology, micro-scale electrodes, i.e., microelectrodes, for the electrical observation of activities in micro-scale environment become realisable. This technique opened a new avenue for micro-scale observation and measurement besides the optical technique. This book presents the techniques, structures, and applications of using microelectrodes in a micro-scale environment. It covers a broad spectrum of topics and the state of the art techniques in the field. As such, the book is divided into four sections, comprising 11 chapters written by recognised experts from leading groups around the world. The reader will gain a unique perspective on the challenges and emerging opportunities in the technology of microelectrodes.

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Book SynopsisFrom the science of bodies to insects and beyond, science is gross and this book is full of fun ways to learn about it. Explore the layers of your skin and or make a model of intestines to figure out the science of poop. Investigate how germs spread, or the makeup of a virus and why snot helps keep you healthy. When you’ve had enough of anatomy, you can take stock of the creepy critters (dustmites!) in your own bed. Emma Vanstone, author of This Is Rocket Science and Snackable Science Experiments, has 60 new activities to investigate everything icky so you can find out exactly how all the smelliest, stickiest, scariest things work. Whether you want to discover the funny things a body does or the ghastly creatures all around you, there’s always something new to learn. Find out what makes your breath so smelly or why stinky foods taste better when you hold your breath. Do your own easy, exciting dissections with homemade models for a brain or heart. Experiment in your own home by making spooky mirror messages or take a terrifying bath with the Bug Bath Bomb. There’s plenty of cool, revolting activities to help you learn about the world. The book has 60 crafting projects and 75 colour photosgraphs.

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Book SynopsisForrest M. Mims is a revered contributor to Make: magazine, where his popular columns about science-related topics and projects for Makers are evergreen treasures. Collected together here for the first time, these columns range from such simple projects as building an LED tracker for hand-launched night rockets to such challenging builds as transforming strings of data into unique musical compositions. A variety of photography and imaging projects are featured, including an ultra-sensitive twilight photometer that measures the elevation of layers of dust, smoke, and smog from around 3,000 feet to the top of the stratosphere at 31 miles! Most of the projects can be done with a collection of simple electronic components, such as LEDs, transistors, resistors, and batteries. To inspire and motivate readers, the book also includes profiles of such famous Makers as President Thomas Jefferson and Microsoft co-founder Paul Allen.

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Book SynopsisThis new volume, Microscopy Applied to Materials Sciences and Life Sciences. focuses on recent theoretical and practical advances in polymers and their blends, composites, and nanocompos¬ites related to their microscopic characterization. It highlights recent accomplishments and trends in the field of polymer nanocomposites and filled polymers related to microstructural characterization. This book gives an insight and better understanding into the development in microscopy as a tool for characterization. The book emphasizes recent research work in the field of microscopy in life sciences and materials sciences mainly related to its synthesis, characterizations, and applications. The book explains the application of microscopic techniques in life sciences and materials sciences, and their applications and state of current research carried out.The book aims to foster a better understanding of the properties of polymer composites by describing new techniques to measure microstructure property relationships and by utilizing techniques and expertise developed in the conventional filled polymer composites. Characterization techniques, particularly microstructural characterization, have proven to be extremely difficult because of the range of length-scales associated with these materials. Topics include:•Instrumentation and Techniques: advances in scanning probe microscopy, SEM, TEM, OM. 3D imaging and tomography, electron diffraction techniques and analytical microscopy, advances in sample preparation techniques in-situ microscopy, correlative microscopy in life and material sciences, low voltage electron microscopy. •Life Sciences: Structure and imaging of biomolecules, live cell imaging, neurobiology, organelles and cellular dynamics, multi-disciplinary approaches for medical and biological sciences, microscopic application in plants, microorganism and environmental science, super resolution microscopy in biological sciences.•Materials Sciences: materials for nanotechnology, metals alloys and inter-metallic, ceramics, composites, minerals and microscopy in cultural heritage, thin films, coatings, surfaces and interfaces, carbon based materials, polymers and soft materials and self-assembled materials, semiconductors and magnetic materials. Polymers and inorganic nanoparticles. The volume will be of significant interest to scientists working on the basic issues surrounding polymers, nanocomposites, and nanoparticulate-filled polymers, as well as those working in industry on applied problems, such as processing. Because of the multidisciplinary nature of this research, the book will be valuable to chemists, materials scientists, physicists, chemical engineers, and processing specialists who are involved and interested in the future frontiers of blends.Table of ContentsScanning Electron Microscopy as a Powerful Morphological Characterization Technique for Polymer Blends and Polymer Nanocomposites. Atomic Force Microscopy: Principles and Applications in Polymer Composites. Barium Strontium Titanate: Preparation, Dielectric, Ferroelectric, and Microscopic Studies. Preparation and Characterization of Copper Thin Films for Antimicrobial Applications. Fabrication, Scanning Electron Microscopic and Electrochemical Studies of Nickel Hydroxide Battery Electrodes Modified with Zinc Oxide. Comparative Spectroscopic Study of Polyindole/Poly(Vinyl Acetate) Composites. Welding of Alloy C-276. Surface Plasmon Resonance Based Sensors. Design Issues in High Strain Rate Dynamic Compressive Failure of Structural Ceramics, Polymers, and Composites. Applications of Electron Backscatter Diffraction (EBSD) in Materials Science. Light Microscopy in Studying of Formation and Decomposition of Wood. Microstructural Analysis of Polymer Blends, Composites, and Nanocomposites. Graphene Modified Carbon Micro-Surfaces in Voltammetric Sensing Applications. Functionalized Nanomaterials for Biological and Catalytic Applications. Removal of Organic Cancer Carcinogens from Wastewater Using Green Synthesis Nanoparticles. Assessing Adipose Tissue Engineering In Vitro and In Vivo: A Microscopic Approach. The Contribution of Light Microscopy to Study Male Reprotoxicity of Cadmium. Microscopy Assessment of Emerging Contaminants Effects on Aquatic Species. Chromium: The Intriguing Element. What Biological Role Has It? (Cr(Iii)-Tris-Picolinate: Is It Safe or Not?). Treatment of Pharmaceutical Wastewater: A Case Study on Degradation in Electrochemical Oxidation.

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Book SynopsisInsects, and their close relatives, the arachnids, centipedes, millipedes and woodlice, make ideal material for study by the recreational microscopist. Moreover for the entomologist, the addition of the use of the microscope to their tool kit adds a whole new dimension to their study, revealing in finest detail the appearance and structure of these tiny creatures. This book reveals the basics of insect microscopy, explaining what equipment is needed and how to get the best out of it. Topics covered include insects and their relatives; trapping insects for study; dissection, slide mounting publishing your work.

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Book SynopsisBored of the same old dinner-party chitchat? Spice up your soirées, impress your guests and show up your brother-in-law with Dangerous Experiments for After Dinner, a book full of hilarious, and sometimes dangerous, after-dinner tricks and challenges.Twenty-one daredevil tricks are illustrated along with step-by-step instructions and explanations of the science behind them. From sabering a bottle of champagne to hammering a needle through a coin, each of these feats is guaranteed to wow your guests.

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Book SynopsisProficiency in volumetric analysis is a key skill for chemists in research and industry. This work seeks to ‘modernise’ approaches to volumetric analysis, by relating practical work to vocationally-relevant topics, whilst maintaining the rigor required for satisfactory performance in practical examinations. Written by someone who has experienced both teaching and working as a research chemist, this up to date textbook on practical volumetric analysis will provide the theoretical chemistry associated with volumetric analysis supported by a selection of practicals. There will also be suggestions for a number of investigations which could form the basis of project-based learning or coursework, particularly for those pursing vocational science courses. Section 1 will consist of three theory chapters, covering preliminary concepts (fundamentals of chemistry, essential quantitative chemistry and concepts of statistics). Section 2 will be divided into four chapters, based on the four main divisions of volumetric analysis (acid-base titrimetry, redox titrimetry, precipitation titrimetry and complexometric titrimetry). Each chapter in this section will start with a review of essential theory, with worked examples and illustrations where appropriate, and end with a selection of laboratory practicals. Each chapter will also contain a number of open-ended investigations, for use in project-based learning or coursework. Section 3 will address more advanced topics and be divided into four chapters (volumetric analysis in industry, further statistical concepts, mathematics of titrimetry and advanced titrimetry). Practical work and suggestions for further reading will be included where appropriate. Practical Volumetric Analysis is suitable for students taking modules in introductory chemistry and analytical chemistry on undergraduate degree courses as well as providing guidance to non-specialists teaching chemistry.Table of ContentsSECTION 1 PRELIMINARIES: Foundations of Chemistry; Essential Quantitative Chemistry; Statistical Concepts; SECTION 2 INTRODUCTORY VOLUMETRIC ANALYSIS: Basics of Volumetric Analysis; Acid-Base Titrimetry; Redox Titrimetry; Precipitation Titrimetry; Complexometric Titrimetry; SECTION 3 FURTHER TOPICS IN VOLUMETRIC ANALYSIS: Advanced Titrimetry; The Mathematics of Titrimetry; Volumetric Analysis in Industry; Further Statistical Concepts; Index

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Book SynopsisHoneybee Anatomy Brought to LifeThis book uses photo essays to set out and display the anatomy, internal, external of the honeybee with over 350 detailed micrographs, together with slide images and drawings. Pests and other additional hive activities are also included. It will appeal to anyone interested in this fascinating insect and be particularly valuable to beekeepers studying for their British Beekeepers Association Module 5 examination.About the authorGraham Kingham is a retired mechanical quality engineer who keeps a few hives in Devon. He started looking down the microscope in 2005 at yeast and bacteria in his beer. To further his interest in microscopy and beekeeping he took the British Beekeeping Association microscopy exam and has continued to explore the fascinating world of the honeybee through the lens. His first bee book was dedicated to the male bee, the drone, followed by an anthology about all things bees.

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Book SynopsisThe first part of this book presents the philosophy behind Kasetsu Hypothesis–Experiment classes and Classbooks (Jugyosho). The second part includes English versions of four of the HEC Classbooks. Teachers are encouraged to find ways of allowing students' own curiosity and thinking to guide their discovery of scientific ideas.Trade ReviewDr. Kiyonobu ITAKURA has inspired researchers and teachers around the world to reconceptualize the use of experimentation in classrooms and instruction. Observing what is known as the Itakura method in action in a Japanese classroom, I saw how it places students at the forefront of the lesson, empowering them to explain their ideas to each other, argue—sometimes vehemently—and reach new insights. May we all honor Dr. Itakura by continuing to refine and extend this method."" — Marcia C. Linn, PhD, Professor, Graduate School of Education, University of California at Berkeley""The book would be a useful addition to the science teacher’s bookshelf. It provides suggestions for a series of well-researched ‘hands on, minds on’ practical activities. The Hypothesis–Experiment Class described is similar in some aspects to the ‘predict-observe-explain’ approach (White & Gunstone, 1992). There is also a useful description of the research carried out in the development of the activities, together with detailed suggestions of how teachers might carry out the activities."" —Mary Whitehouse, Chair, The Association for Science Education""Kiyonobu Itakaura’s first scientific paper, written in 1953 as a graduate student, was exciting to read. He emphasized that geocentric theory persisted for centuries not just because of social reasons but because it was supported by most astronomers. He taught me the vital importance of learning that “truth is not determined by the majority”. This lesson is still pertinent for scientists today as dogmatism continues to play a role in confounding scientific judgement. I hope that HEC will also inspire students to think for themselves."" —Toshimitsu Yamazaki, Dr. of Science, Professor Emeritus, University of Tokyo, Member of the Japan AcademyTable of Contents Preface Acknowledgements Biography of Dr. Kiyonobu Itakura About the editorial committee members A Collection of Articles and Essays by Kiyonobu Itakura 1 The Process of Establishing Mental Recognition ??in Science 2 What is the Hypothesis–Experiment Class? ??History and Classroom Management 3 Hypothesis–Experiment Class as Democratic Education 4 Memorandum Regarding Hypothesis–Experiment Class First Appearances and Research History Further Reading The Kasetsu Class Album Appendices: HEC Classbooks (Jugy?sho) 1 Objects and their Weight 2 Force and Motion (1) 3 If You Could See an Atom 4 How Many Legs?

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Book Synopsis

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Book Synopsis

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Book Synopsis

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Book SynopsisThis book features reviews by leading experts on the methods and applications of modern forms of microscopy. The recent awards of Nobel Prizes awarded for super-resolution optical microscopy and cryo-electron microscopy have demonstrated the rich scientific opportunities for research in novel microscopies. Earlier Nobel Prizes for electron microscopy (the instrument itself and applications to biology), scanning probe microscopy and holography are a reminder of the central role of microscopy in modern science, from the study of nanostructures in materials science, physics and chemistry to structural biology.Separate chapters are devoted to confocal, fluorescent and related novel optical microscopies, coherent diffractive imaging, scanning probe microscopy, transmission electron microscopy in all its modes from aberration corrected and analytical to in-situ and time-resolved, low energy electron microscopy, photoelectron microscopy, cryo-electron microscopy in biology, and also ion microscopy. In addition to serving as an essential reference for researchers and teachers in the fields such as materials science, condensed matter physics, solid-state chemistry, structural biology and the molecular sciences generally, the Springer Handbook of Microscopy is a unified, coherent and pedagogically attractive text for advanced students who need an authoritative yet accessible guide to the science and practice of microscopy.Trade Review“This book highlights the unity of electron microscopy, X-ray microscopy and optical microscopy … . This handbook is a recommended source of information on microscopy theory, instrumentation, applications, limitations, trade-offs and pitfalls. … The contributors have produced outstanding chapters, which should be noted for their comprehensive analysis of the theory and the instruments, their critical selection of figures, and their selection of key references.” (Barry R. Masters, Optics & Photonics News, April 9, 2020)Table of ContentsPart A: Electron and Ion Microscopy.- Kirkland et al.: Atomic Resolution Transmission Electron Microscopy.- Nellist: Scanning Transmission Electron Microscopy.- Ross & Minor: In situ Transmission Electron Microscopy.- Plitzko & Baumeister: Crytoelectron TEM.- Erdmann et al: Scanning Electron Microscopy.- Thiel: Variable Pressure Scanning Electron Microscopy.- Botton, Pradhudev: Analytical Electron Microscopy.- Campbell et al: High-Speed Electron Microscopy.- Bauer: LEEM, SPLEEM and SPELEEM.- Feng & Scholl: Photoemission Electron Microscopy.- Tromp: Spectroscopy with the Low Energy Electron Microscope.- Van Aert: Model-Based Electron Microscopy.- Hawkes & Krivanek: Aberration Correctors, Monochromators, Spectrometer.- Hlawacek: Ion Microscopy.-Kelly: Atom-Probe Tomography.- Part B: Holography, Ptychography and Diffraction.- Dunin-Borkowski et al.: Electron Holography.-Rodenburg & Maiden: Ptychography.- Zuo: Electron Nanodiffraction.- Musumeci & Li: High-Energy Time-Resolved Electron Diffraction.- Spence: Diffractive Imaging of Single Particles.- Part C: Photon-based Microscopy.- Diaspro et al: Fluorescence Microscopy.- Sahl et al.: Far-Field Fluorescence Microscopy.- Jacobson et al: Zone-Plate X-Ray Microscopy.- Lin et al: Microcomputed Tomography.- Part D: Applied Microscopy.- Huey et al: Scanning Probe Microscopy in Materials Science.- Leary & Midgeley: Electron Tomography in Materials Science.- Sutter: Scanning Tunneling Microscopy in Surface Science.- Hamidian et al: Visualizing electronic quantum matter.- Ma et al (Terasaki): Microscopy of Nanoporous Crystals.- Wen: Biomedical X-Ray Phase-Contrast Imaging and Tomography.- Amrein & Stamov: Atomic Force Microscopy in the Life Sciences.- Jones: Microscopy in Forensic Sciences.

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Book SynopsisThis book offers a beginner’s guide to using light microscopes. It begins with a brief introduction to the physics of optics, which will give the reader a basic grasp of the behaviors of light. In turn, each part of the microscope is explained using clear and simple English, together withdetailed photographs and diagrams. The reader will learn the function, care and correct use of each part. A troubleshooting section also helps resolve some of the most common issues encountered in light microscopy. Most people have a general idea of how to use a microscope, but many never get the full benefit, because they receive no training. With easy-to-follow steps and detailed images, this guide will help everyone achieve the best results, and be confident using their microscope. This book is intended for anyone using a light microscope, such as university students, people in lab environments, hobbyists, educators who teach science to young children, and anyone with a general interest in these valuable tools. Table of Contentssee attachment

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Book SynopsisThis textbook shall introduce the students to 3D cell culture approaches and applications. An overview on existing techniques and equipment is provided and insight into various aspects and challenges that researchers need to consider and face during culture of 3D cells is given. The reader will learn the importance of physiological cell, tissue and organ models and gains important knowledge on 3D analytics. This textbook deepens selected aspects of the textbook “Cell Culture Technology”, which also is published in this series, while offering extended insight into 3D cell culture. The concept of the textbook encompasses various lectures ranging from basics in cell cultivation, tissue engineering, biomaterials and biocompatibility, in vitro test systems and regenerative medicine. The textbook addresses Master- and PhD students interested and/or working in the field of modern cell culture applications and will support the understanding of the essential strategies in 3D cell culture and waken awareness for the potentials and challenges of this application.Table of ContentsChapter 1. Introduction to 3D cell culture.- Chapter 2. Lab equipment for 3D cell culture.- Chapter 3. A view from the cellular perspective.- Chapter 4. Biological, natural and synthetic 3D matrices.- Chapter 5. Hydrogels for 3D cell culture.- Chapter 6. Vascularization in 3D cell culture.- Chapter 7. Application of scaffold-free 3D models.- Chapter 8. 10. Microfluidic Systems and Organ (Human) on a Chip.- Chapter 9. 3D-Bioprinting.- Chapter 10. Non-destructive and label-free monitoring of 3D cell constructs.

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Book SynopsisThis book covers fundamental microscopic techniques for Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Atomic Force Microscopy (AFM), and other microscopic tools. It provides step-by-step instructions and explanations of the basic fundamental concepts and mechanisms and guides the reader on resolving queries related to taking and analyzing microscopy images. The latest advancements and developments in microscopic equipment are described. Theoretical background on microscopy is also provided to enhance the reader’s understanding of microscopy techniques and tools. Microscopic Techniques for the Non-Expert is an ideal book for undergraduate and postgraduate students, as well as researchers with a background in environmental science, materials science, biomedicine, engineering, or bio-nanotechnology.Table of ContentsA Beginner’s Guide to Different Types of Microscopes Principles of Light and Fluorescence Microscopy Confocal and Multiphoton Microscopy Scanning Probe Microscopy: tipping the path towards atomic visions Atomic Force Microscopy - An Advanced Imaging Technique: From Molecules to Morphologies Exploring the microcosm at atomic precision using Atomic Force Microscopy Scanning Electron Microscopy (SEM): Learning to generate and interpret the topographical aspects of materials Recent updates on methods, applications, and practical uses of scanning electron microscopy in various life sciences Transmission electron microscopy - A powerful and novel scientific technique with nanoscale resolution for characterization of materials Preparation of Biological Samples for SEM: Techniques and Procedures

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Book SynopsisThe Covid-19 pandemic changed the world. Indeed a real race took place worldwide between SARS-CoV-2 on the one hand and researchers on the other – especially those specializing in messenger RNA vaccines. Four years after its emergence, the pandemic is not over, but some decisive battles have been won, thanks to the great success of mRNA vaccines. The Marathon of The Messenger presents the history of these mRNA vaccines, combining a scientific background with historical and economic perspectives. It appears that an important page in the history of these new vaccines was written in Europe, thanks to the crucial work of German and French scientists; this effort began in 1993 and continues to this day. In the face of a prevailing single-mindedness, these researchers pushed through a new therapeutic concept and defined the biotechnological keys that would open the way to the production of therapeutic messenger RNA in the fight against cancer and viral infections. Written for a broad audience and accompanied by humorous cartoons, this book will appeal to anyone looking for scientific and historical answers about mRNA vaccines. Readers will discover not only the technical and scientific knowledge of how these vaccines work, but also the economic levers that were necessary to create this technology. This book has been written in collaboration with Dr. Steve Pascolo, former director of CureVac, and the RNA messenger expert Professor Chantal Pichon. It also features a preface by Dr. Pierre Meulien, former director of the European Union public-private partnership Innovative Medicines Initiative (IMI).Table of ContentsChapter 1. Introduction: The Marathon of the Messenger RNA.- Part I. Messenger RNA, an essential role and challenge.- Chapter 2. A short history of vaccination.- Chapter 3. From DNA to RNA.- Chapter 4. Messenger RNA (mRNA): from transcription to protein translation.- Chapter 5. The mechanism of viral infection.- Chapter 6. The immune response.- Part II. From preliminary studies to clinical trials.- Chapter 7. Promising studies in the 1990s.- Chapter 8. The use of mRNA - the initial technical obstacles.- Chapter 9. The birth of CureVac: the era of the pioneers.- Chapter 10. Solutions for mRNA optimization.- Chapter 11. Modified and unmodified mRNA: for what purpose?.- Chapter 12. Experiments and clinical trials conducted: the power of therapeutic mRNA.- Chapter 13. Experiments and clinical trials against infectious diseases.- Chapter 14. Experiments and clinical trials against cancer.- Chapter 15. Experiments and clinical trials carried out in other therapeutic fields.- Chapter 16. Modified mRNA versus unmodified mRNA: not just a scientific issue.- Part III. The triumph of messenger RNA.- Chapter 17. A very progressive diffusion in scientific circles.- Chapter 18. The development of future biotechs in the 2010s.- Chapter 19. The importance of intellectual property and patents.- Chapter 20. 2020: the triumph of anti-Covid mRNA vaccines.- Chapter 21. mRNA, a technology of the future

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Book SynopsisFor most people locations that hold a particular importance for the development of our society and for the advancement of science and technology often remain hidden from view. They are separate and protected, such as CERN, the European Organization for Nuclear Research, close to the city of Geneva. CERN is best known for its giant particle accelerator. Here researchers from around the world take part in a diverse array of fundamental physical research, in the pursuit of knowledge that will perhaps one day revolutionize our understanding of the universe and life on our planet. The Swiss photographer Andri Pol mixed with this multicultural community of researchers and followed their work over an extended period of time. In doing so he created a unique portrait of this fascinating underworld.A" The cutting-edge research is given a human face and even if we don't fully understand the processes at work, the pictures allow us to perceive how in this world of the tiniest particles the biggest connections are searched for. With an explanatory text and scientific-philosophical essay.

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Book SynopsisWe cannot imagine a world without plastics. Plastic products make our daily life safe, healthy and convenient. Besides all the benefits, the current plastics economy gives rise to environmental concerns with respect to fossil oil depletion and plastic waste accumulation. In a circular economy, however, plastics can be redesigned for reusability and recyclability. This book makes the topic of sustainable plastics approachable for students and career starters alike, describing the nature and chemistry of (bio)polymers as well as how to create a closed loop of plastic materials.

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Book SynopsisA classic brought up to date with new experiments using the latest methods. Modern spectroscopic techniques and current research topics make this an incomparable resource for undergraduate and graduate students, presenting a fascinating approach to inorganic chemistry by providing experiments that resemble real research. As a result, students learn to think in a research-oriented fashion and to research together in a group. The experiments have been thoroughly tested and safety instructions are included, while hazardous substances are replaced by less harmful ones. This new edition also has a special focus on environmentally friendly experiments. Trade Review"In my view the flexible, expertly informed approach that this book offers makes it essential reading foracademic and technical staff who are planning a modern inorganic course." (Education in Chemistry , 1 March 2011) "Like the first and second editions of Inorganic experiments, the third edition is clearly and concisely written and excellently produced." (Chemistry World, 1 February 2011)Table of ContentsIntroduction Introductory Experiments Intermediate Experiments Advanced Experiments

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Book SynopsisThis textbook helps you to prepare for your next exams and practical courses by combining theory with virtual lab simulations. The Labster Virtual Lab Experiments series gives you a unique opportunity to apply your newly acquired knowledge in a learning game that simulates exciting laboratory experiments. Try out different techniques and work with machines that you otherwise wouldn't have access to.In this book, you'll learn the fundamental concepts of basic biochemistry focusing on:Ionic and Covalent BondsIntroduction to Biological MacromoleculesCarbohydratesEnzyme KineticsIn each chapter, you'll be introduced to one virtual lab simulation and a true-to-life challenge. Following a theory section, you'll be able to play the relevant simulation that includes quiz questions to reinforce your understanding of the covered topics. 3D animations will show you molecular processes not otherwise visible to the human eye. If you have purchased a printed copy of this book, you get free access t

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Book SynopsisDieses Einsteiger-Lehrbuch bietet eine hochaktuelle, aber auch alltagstaugliche Gesamtschau der Bioanalytik. Drogen- und Virus-Tests, die Blutzucker-Bestimmung bei Diabetes, die Messung der körperlichen Fitness, Schwangerschaft und der lebensrettende Check eines Herzinfarkts sind einige der spannenden und lebensnahen Aspekte, die mit einer Vielzahl an vierfarbigen Grafiken und Fotos beschrieben werden. Wie das mit bereits fünf Auflagen erfolgreiche Werk des Autors Biotechnologie für Einsteiger soll auch dieses Buch zeigen: „Wissenschaft kann Spaß machen!" und die Neugier auf mehr wecken – und das schon alleine beim Durchblättern. Die abwechslungsreiche Gestaltung des Buches bietet neben Meinungen von Experten, die Standpunkte aus Forschung und Industrie veranschaulichen, auch geschichtliche Aspekte sowie die beliebte Nanoru-Cartoon-Geschichte, die, wie gewohnt, witzig in die Thematik einführt. Acht Fragen am Ende jedes Kapitels helfen bei der Selbstkontrolle von Lernbegierigen.Die zweite Auflage wurde gänzlich überarbeitet und – buchstäblich bis zur letzten Minute vor dem Druck – u.a. mit dem erläuterten Nachweis von Coronaviren auf den aktuellsten Stand gebracht.Table of ContentsDas Nanoru.- Biomoleküle auf dem Prüfstand.- Biokatalyse.- Bio-Affinität I.- Bio-Affinität II.- DNA, RNA und ihre Amplifikation.- Biosensoren.

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Book SynopsisDas bewährte Standardwerk Bioanalytik beschreibt und erläutert alle analytischen Methoden, die heute in der Biochemie und Molekularbiologie eingesetzt werden - in fünf großen Abschnitten: Proteinanalytik, 3D-Strukturaufklärung, Spezielle Stoffgruppen, Nucleinsäureanalytik, Systematische Funktionsanalytik. Die komplett überarbeitete 3. Auflage berücksichtigt zahlreiche methodische Weiterentwicklungen und greift auch hochaktuelle Trends in der Forschung auf. Großer Wert wurde auf eine kritische, praxisbezogene Darstellung der Methoden und auf eine Vernetzung der verschiedenen Kapitel untereinander gelegt. So wird die Neuauflage dieses kompetenten und informationsreichen Lehr- und Handbuches wieder all jenen, die sich in der Vielfalt der biologisch-chemischen Labormethoden zurechtfinden müssen, als zuverlässiger Wegweiser dienen.Table of Contents1 Bioanalytik - eine eigenständige Wissenschaft.- Teil I Proteinanalytik.- 2 Proteinreinigung.- 3 Proteinbestimmungen.- 4 Enzymatische Aktivitätstests.- 5 Mikrokalorimetrie.- 6 Immunologische Techniken.- 7 Chemische Modifikation von Proteinen und Proteinkomplexen.- 8 Spektroskopie.- 9 Lichtmikroskopische Verfahren – Imaging.- 10 Spaltung von Proteinen .- 11 Chromatographische Trennmethoden.- 12 Elektrophoretische Verfahren.- 13 Kapillarelektrophorese.- 14 Aminosäureanalyse.- 15 Proteinsequenzanalyse.- 16 Massenspektrometrie.- 17 Massenspektrometriebasierte Immunassays.- 18 Bildgebende Massenspektrometrie.- 19 Protein-Protein-Wechselwirkungen.- 20 Bio- und biomimetische Sensoren.- Teil II 3D-Strukturaufklärung.- 21 Magnetische Resonanzspektroskopie von Biomolekülen.- 22 EPR-Spektroskopie an biologischen Systemen.- 23 Elektronenmikroskopie.- 24 Rasterkraftmikroskopie.- 25 Röntgenstrukturanalyse.- Teil III Spezielle Stoffgruppen.- 26 Analytik synthetischer Peptide.- 27 Kohlenhydratanalytik.- 28 Lipidanalytik.- 29 Analytik posttranslationaler Modifikationen: Phosphorylierung und oxidative Cysteinmodifikation von Proteinen.- Teil IV Nucleinsäureanalytik.- 30 Isolierung und Reinigung von Nucleinsäuren.- 31 Aufarbeitung und chemische Analytik von Nucleinsäuren.- 32 RNA-Strukturaufklärung durch chemische Modifikation.- 33 Polymerasekettenreaktion.- 34 DNA-Sequenzierung.- 35 Analyse der epigenetischen Modifikationen.- 36 Protein-Nucleinsäure-Wechselwirkungen.- Teil V Systematische Funktionsanalytik.- 37 Sequenzanalyse.- 38 Hybridisierung fluoreszenzmarkierter DNA zur Genomanalyse in der molekularen Cytogenetik.- 39 Physikalische, genetische und funktionelle Kartierung des Genoms.- 40 DNA-Microarray-Technologie.- 41 Silencing-Technologien zur Analyse von Genfunktionen.- 42 Proteomanalyse.- 43 Metabolomics- 44 Interactomics - systematische Protein-Protein-Wechselwirkungen.- 45 Chemische Biologie.- 46 Toponomanalyse.- 47 Organ-on-Chip.- 48 Systembiologie.- Anhang

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Book SynopsisThis second volume of the Applied Human Cryobiology series contains presentations on the second German scientific symposium 2014 in Dresden as well as contributions of outstanding scientists in cryonics. Included are laudations to the awardees of the Robert Ettinger Medal. The brain as the only totally individualized human organ cannot be replaced (e.g. by cloning or stem cells). Therefore analyses of brain structure as well as studies in the postmortem stability of this organ are crucial for methods of vitrification and the rewarming of cryonics patients. Other organs and organisms are useful models for the development and testing of cryopreservation methods. These require strategies for the control and prevention of cryopreservation damage as well as damage caused by dying. New technologies can help to achieve these aims. An important field of research in this context is molecular repair. The further development of cryonics needs self-control, e.g. by analysis of its historical development and actual progress. Cryonics represents a method of life span extension and can be supported by other methods favoring longevity. This volume demonstrates that substantial progress has been made in all of these fields of research as well as in the application of the results of this research.

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Book SynopsisAprender las operaciones básicas matemáticas —suma, resta, multiplicación y división— es realmente sencillo y divertido con ayuda de los mayas, No hace falta memorizar tablas. Solo necesitas seguir tu intuición, usar los mismos puntos, rayas y caracoles que utilizaban los antiguos mayas y seguir las indicaciones y ejemplos que te proponemos con ayuda de los tableros que encontrarás dentro del libro. Verás que las matemáticas, además de divertidas, son fáciles de aprender y rápidas y de utilizar.

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Book SynopsisLas fracciones tienen muchos usos en la vida diaria, desde medir longitudes, capacidades y pesos, hasta comparar fracciones, encontrar fracciones equivalentes y realizar operaciones. Este libro trata de exponer todas las maneras posibles de enseñar el tema, acompañadas de actividades divertidas y juegos. El libro incluye un CD con ejercicios y soluciones; así como diez bandas de colores divididas en medios, tercios, cuartos, etcétera.

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Book Synopsis

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Book Synopsis

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Book Synopsis

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Book Synopsis

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Book SynopsisThe Voyage of the Beagle is a seminal work by means of the famend British naturalist Charles Darwin, recounting his 5-12 months' journey aboard the HMS Beagle, from 1831 to 1836. The book presents a charming and insightful account of the voyage, which took him round the sector and played a pivotal position within the development of his idea of evolution by herbal choice. The narrative takes readers on a systematic expedition, exploring the vegetation, fauna, geology, and various cultures encountered in South America, the Galápagos Islands, Australia, and other areas. Darwin's meticulous observations and the wealth of records accrued during the voyage laid the muse for his groundbreaking work, On the Origin of Species. In The Voyage of the Beagle, Darwin demonstrates his keen powers of statement and his capacity to synthesize geological and organic information. He offers a detailed report of the animals and plants he encountered, in conjunction with the geological formations and the insights gained from interactions with indigenous peoples. The book serves as a travelogue, herbal records account, and scientific exploration blended into one, presenting readers a glimpse into the thoughts of a younger scientist at the cusp of one of the most transformative medical discoveries in history.

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Book SynopsisThe Microscope is a seminal paintings authored by Andrew Ross, a outstanding English optician and microscope maker of the 19th century. This comprehensive e-book is a sizable contribution to the sphere of microscopy and clinical instrumentation. In this pioneering paintings, Ross delves into the principles, production, and realistic packages of microscopes. He offers distinctive insights into the design and mechanics of microscopes, in conjunction with instructions on how to use them successfully for clinical statement and research. The Microscope not handiest serves as a treasured guide for the ones inquisitive about microscopy but also highlights the essential role of microscopes in advancing medical understanding. Ross's meticulous descriptions and illustrations of microscope components and techniques made microscopy greater handy to a broader audience, inclusive of scientists, naturalists, and students. Moreover, Ross's work reflects the enthusiasm and curiosity of the generation's clinical network, as microscopes were instrumental in coming across previously unseen worlds and phenomena. His book contributed to the popularization of microscopy and the speedy advancements in organic and medical research throughout the nineteenth century. The Microscope via Andrew Ross stays a undying aid for anybody inquisitive about the history and realistic applications of microscopes.

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Book SynopsisEl autor muestra cómo la práctica del zen puede arrojar luz sobre los evangelios de Jesús y ayudar a que uno viva de manera más alegre y compasiva. La compasión de Buda y el amor de Cristo tienen mucho en común y el camino que propone el budismo zen puede acercarnos a una experiencia más plena de Dios, de nosotros mismos y de nuestro entorno.

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Book SynopsisBasic principles of applied life sciences such as recombinant DNA technology is used in most life sciences industries marketing bio-formulations for designing more effective protein-based drugs, such as erythropoietin and fast-acting insulin etc. In recent times genetically engineered host cells from mammal, animal and plants are also being used in life sciences industries to manufacture biologics. This book discusses the most basic as well advanced issues on biological products for successfully managing a life sciences industry. It elucidates the life cycle of biological molecules, right from the conceptual development of different types of biopolymers, and their subsequent transfer from the conical flasks in laboratory to life sciences industries for large scale production and marketing. It focuses on sustainable longevity in the life cycle of commercial biopolymers. Cumulative facts and figures in this volume would immensely help in inspiring life sciences industry promoters to monitor value chain transfer process of biologics for better profitability. Additionally, it would serve as a perusal document for the students and researchers interested in entrepreneurial ventures or having their own start-up projects for the commercialization of biologics. Table of ContentsChapter 1. Prelusion Significance of Livestock.- Chapter 2. Mass Balance concept in Livestock Farming.- Chapter 3. Goat and Sheep Farming.- Chapter 4. Cattle and Buffaloes Farming.- Chapter 5. Factors Influencing Livestock Way of Life.- Chapter 6. Sustainable Livestock Farming.- Chapter 7. Conceptual Development of Livestock Supply Chain Management.

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Book SynopsisThis book summarizes the related research achievements in Antibody-drug conjugates (ADCs) and their cell metabolism kinetics. The book has three main parts. The first part describes the basic theory of ADCs, including the basic concept and structure of ADCs, and the relationship between the targets of ADCs and their specific functions. The second part mainly introduces the endocytosis and intracellular metabolism of ADCs, including the relationship between endocytosis and ADC activity, the endocytosis and intracellular transport of ADCs, the distribution and metabolism of ADC in vivo. Then it discusses the new formats and research technology of ADCs, including the application of miniaturized antibodies in ADC synthesis, novel carriers for ADC design, the technology and application of site-specific conjugation, and approaches for analyzing the drug: antibody ratio (DAR), the study of pharmacokinetics of ADCs. This book combines the basic theory with the research technology. It can be used as a reference book for students, teachers and researchers of biomedical field.Table of ContentsAntibody-Drug Conjugates Basic Concepts and Structures.- Relationship between target and specific action of antibody-drugs conjugates.- The internalization and therapeutic activity of antibody drug conjugate.- The internalization and intracellular trafficking of ADC.- Distribution and metabolism of antibody-drug conjugates.- Application of Antibody fragments in ADCs.- Novel Targeting Carriers in Antibody-drug Conjugates.- Site-specified Conjugating Technology and Application.- Determination of drug-to-antibody ratio of ADCs.- Pharmacokinetic study of antibody-drug conjugates.

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