Genetics (non-medical) Books
Springer The Management and Health of Farmed Deer: A Seminar in the CEC Programme of Coordination of Research in Animal Husbandry, held in Edinburgh on 10–11 December 1987
Book SynopsisThe farming of deer as an alternative to traditional livestock enterprises is now firmly established and is expanding within several countries of the European Economic Community. However, the successful farming of deer requires the adoption of appropriate management schemes to accommodate the biological requirements of these animals. Much experience has now been gained and it is essential that this information becomes readily available througout the Community. In addition, as the volume of deer farming has increased a number of health problems have become recognised which present features distinct from other domestic ruminants. Although knowledge is still incomplete it would appear that deer may react to certain pathogens in a very different way to other domestic ruminants, presenting new problems of diagnosis and control. The rapid detection of these conditions and development of appropriate control strategies will be essential for the establishment of an economically viable deer farming industry in the Community. Much of the information on the management of farmed deer and their diseases is anecdotal and fragmented and the purpose of this meeting was to accelerate the dissemination of this knowledge between scientists in the Community committed to the development of this area of agricultural industry. The meeting, financed by the Commission of the European Communities from its budget for the Coordination of Agricultural Research in the Community was held in Scotland, on the 10th to 11th December, 1987.Table of ContentsManagement and Nutrition of Farmed.- Session I.- Studies on The Epidemiological Pattern and Control of Nematode Infection in Cerv/Idae.- Epidemiology and Control of Parasitic Diseases in Danish Deer Farms.- Pulmonary Parasites: Pathology and Control.- Cryptosporidiosis in Red Deer.- Conclusions From Session I.- Session II.- General and Economic Aspects of Deer Farming.- Deer Farming in Denmark, With Special Emphasis on The Management and Handling of Fallow Deer.- Diagnostic Examinations of Autopsy Material Submitted From Farmed Deer in Denmark.- Slaughter of Deer.- Conclusions From Session II.- Session III.- Health Aspects of Deer Farming.- Tuberculosis in Farmed Red Deer.- Paratuberculosis.- Experience on A Scottish Experimental Deer Farm.- Serological Survey in Free-Living Red Deer in France.- Ethological Aspects of Deer Farming.- Conclusions From Session II.- Session IV.- Studies on The Epidemiology and Pathogenisis of Alphaherpesviruses From Red Deer and Reindeer.- Herpesvirus Infections of Red Deer and Other Wild Ruminants in France and Belgium.- The Diagnosis of Malignant Catarrhal Fever in Deer.- The Aetiology of Malignant Catarrhal.- Conclusions From Session iv.- Session V.- The Transport of Deer.- Early Nutrition, Growth and Reproductive Performance In Young Scottish Red Deer Hinds, and Their Economic Significance in Commercial Herds.- Farm Tour of Macaulay Land Use Research Station At Glensaugh: A Summary of The Main Features.- Conclusions From Session V.- Final Conclusion.- List of Participants.
£42.74
Pan Stanford Publishing Pte Ltd MicroRNA Profiling in Cancer: A Bioinformatics
Book SynopsisThis book presents advances in the interdisciplinary field of microRNA research of human cancers from a unique perspective of quantitative sciences: bioinformatics, computational and systems biology, and mathematical modeling. This volume contains adaptations and critical reviews of state-of-the-art studies, ranging from technological advances in microRNA detection and profiling, clinically oriented microRNA profiling in several human cancers, to a systems biology analysis of global patterns of microRNA regulation of signaling and metabolic pathways. It discusses interactions with transcription factor regulatory networks and mathematical modeling of microRNA regulation.Trade Review"Topics on microRNAs have taken scientists by storm in the last seven years. Today more than 5500 publications related to microRNAs can be found in PubMed. Do we really need another book for readers already saturated with this topic? The answer is a definite yes. Yuriy Gusev has wisely put together a fine book linking various aspects of the study of microRNAs starting with profiling of microRNoma, the functional study of pathway networks involving miRNAs as well as evolutionary models of microRNoma evolution or mathematical models of posttranscriptional regulation of microRNAs. Readers will find the book different compared with others already published. One thing is certain: the curiosity of the readers will increase with every page turned."—George A. Calin, MD Anderson Cancer CenterTable of ContentsMeasurement and Interpretation of MiRNA Expression Profiles. MicroRNA Expression Analysis by LNA Enhanced Microarrays. Analysis and QC for Real-Time QPCR Arrays of Human MicroRNAs. Massively Parallel MicroRNA Profiling in the Haematologic Malignancies. Who Dunit? MicroRNAs Involved in Prostate Cancer. Intronic MicroRNA. Creation, Evolution and Regulation. Mirome Architecture and Genomic Instability. Interconnection of MicroRNA and Transcription Factor-Mediated Regulatory Networks. Analysis of MicroRNA Profiling Data with Systems Biology Tools. Mathematical and Computational Modeling of Post-Transcriptional Gene Regulation by MicroRNAs.
£93.09
Pan Stanford Publishing Pte Ltd Cancer Genetics and Genomics for Personalized
Book SynopsisThis book covers almost all fields of cancer genetics and genomics for personalized medicine. Targeted therapy, or precision medicine, or personalized medicine is becoming a standard treatment for many diseases, including cancer. However, how much do we know about the personalized medicine approach? This lucid book helps undergraduate and graduate students, professional researchers, and clinicians to better understand the key concept of personalized medicine.The most up-to-date topics on personalized medicine in this book cover the recent trends in and updates on lung, gastric, liver, breast, and other types of cancers. Circulating tumor cell, cell-free circulating DNA, and microRNAs are discussed as new diagnostic and prognostic markers for cancer. The avatar mouse model is also discussed for maximizing treatment efficacy and prognosis prediction, and so is microenvironment as a drug resistance mechanism. With classical and new pathological approaches, the book provides a systemic overview of personalized immunotherapies and hyperthermic intraperitoneal chemotherapy, followed by new emerging fields of hereditary cancer, thereby equipping readers to eventually contribute in developing more advanced tools and therapies for curing cancer.Trade Review"Likely as the recent Cancer Moonshot Initiative highlights, this book well describes the importance of genetics/genomics-based personalized therapies to improve cancer treatments. This field is growing rapidly, and it is certain that many scientists should obtain professional knowledge of ‘cancer precision medicine’ to cure more cancer patients."—Prof. Yusuke Nakamura, University of Chicago, USA"At a time when an explosive increase in data has combined with rapidly evolving treatment paradigms, it has become particularly challenging for modern clinicians and medical researchers alike to put these advances in knowledge and practice into a proper context. It is in light of this predicament that this new volume edited by Dr. Il-Jin Kim provides a much-needed organization and harmonic understanding to the cacophony of information regarding the elusive realm of precision medicine. Covering an exhaustive array of topics ranging from the basic science of tumor modeling to the biology of tumor microenvironments to the horizon of cancer immunotherapy and advances for specific cancer types, this clearly written and very readable compilation provides a solid foundation for comprehending the rapidly advancing dawn of a truly new, personalized, precision approach to the treatment and, hopefully, the cure of cancer."—Prof. Michael J. Mann, University of California, San FranciscoTable of ContentsPersonalized Medicine for Cancer: Introduction and Overview of the Book. Personalized Medicine in Lung Cancer. Genome-Based Personalized Medicine in Liver Cancer. Applications of Circulating DNA Analysis in Personalized Medicine. Circulating Tumor Cells and Personalized Medicine. Mouse Models in Personalized Cancer Medicine. Tumor Microenvironment, Therapeutic Resistance, and Personalized Medicine. Personalized Immune Therapy. Hyperthermic Intraperitoneal Chemotherapy (HIPEC) for Peritoneal Malignancies. Personalized Medicine in Hereditary Cancer Syndromes. Pathology in the Era of Personalized Medicine. MicroRNAs in Human Cancers. Pharmacogenomics of Tamoxifen.
£102.60
The University of Chicago Press Genomes and What to Make of Them
Book SynopsisFor the nonscientist, the claims and counterclaims are dizzying - what does it really mean to understand the genome? In this title, the authors offer an answer to this question and many more in a clear account of the genomic revolution and its promise.
£17.66
The University of Chicago Press Playing God
Book SynopsisTechnology evolves at a dazzling speed, particularly in the field of genetic engineering. But the public has not had much to say about the advancements in human genetics. This text asks why and explores the social forces that have led to the thinning out of public debate over genetic engineering.
£76.95
The University of Chicago Press Playing God Human Genetic Engineering and the
Book SynopsisTechnology evolves at a dazzling speed, particularly in the field of genetic engineering. But the public has not had much to say about the advancements in human genetics. This text asks why and explores the social forces that have led to the thinning out of public debate over genetic engineering.
£30.40
The University of Chicago Press Fins into Limbs
Book Synopsis
£47.50
The University of Chicago Press The Lysenko Affair
Book SynopsisThe Lysenko affair was perhaps the most bizarre chapter in the history of modern science. For thirty years, until 1965, Soviet genetics was dominated by a fanatical agronomist who achieved dictatorial power over genetics and plant science as well as agronomy. A standard source both for Soviet specialists and for sociologists of science.American Journal of Sociology Joravsky has produced . . . the most detailed and authoritative treatment of Lysenko and his view on genetics.New York Times Book Review
£38.00
The University of Chicago Press Lords of the Fly
Book SynopsisArgues that the fly was essentially a laboratory tool whose productivity opened many new lines of genetic research. Kohler also explores the moral economy of the Drosophilists. By closely examining their culture and customs, he reveals features of how experimental scientists do their work.
£34.20
The University of Chicago Press Truth Machine The Contentious History of DNA
Book SynopsisDNA profiling is often heralded as unassailable criminal evidence, a veritable "truth machine" that can overturn convictions based on eyewitness testimony, confessions, and other forms of forensic evidence. This book traces the controversial history of DNA fingerprinting by looking at court cases in the US and UK from the mid-1980s onwards.Trade Review"I could not put it down. This is a must-read for anyone interested in the history of science." (Times Higher Education) "An interesting read.... It illustrates that the controversy of DNA profiling is rooted not in the science, but mainly in the restrictions of the adversarial system." (Nature)"
£26.00
The University of Chicago Press The Theory of Evolution
Book Synopsis
£37.05
The University of Chicago Press Sewall Wright and Evolutionary Biology Science
Book SynopsisProvine's thorough and thoroughly admirable examination of Wright's life and influence, which is accompanied by a very useful collection of Wright's papers on evolution, is the best we have for any recent figure in evolutionary biology.Joe Felsenstein, NatureIn Sewall Wright and Evolutionary Biology . . . Provine has produced an intellectual biography which serves to chart in considerable detail both the life and work of one man and the history of evolutionary theory in the middle half of this century. Provine is admirably suited to his task. . . . The resulting book is clearly a labour of love which will be of great interest to those who have a mature interest in the history of evolutionary theory.-John Durant, ;ITimes Higher Education Supplement;X
£38.00
The University of Chicago Press Heredity under the Microscope
Book SynopsisBy focusing on chromosomes, Heredity under the Microscope offers a new history of postwar human genetics. Today chromosomes are understood as macromolecular assemblies and are analyzed with a variety of molecular techniques. Yet for much of the twentieth century, researchers studied chromosomes by looking through a microscope. Unlike any other technique, chromosome analysis offered a direct glimpse of the complete human genome, opening up seemingly endless possibilities for observation and intervention. Critics, however, countered that visual evidence was not enough and pointed to the need to understand the molecular mechanisms. Telling this history in full for the first time, Soraya de Chadarevian argues that the often bewildering variety of observations made under the microscope were central to the study of human genetics. Making space for microscope-based practices alongside molecular approaches, de Chadarevian analyzes the close connections between genetics and an array of scieTrade Review"The chromosome is at least as powerful an icon of heredity as DNA but so far has not held a central place in the historiography of twentieth-century life sciences. [Heredity under the Microscope] sets this record straight by directing its lense on the cytogenetic study of human chromosomes and the many contexts in which chromosome images came to matter. It opens fascinating new perspectives on the 'century of the gene' that go far beyond the earlier focus on the history of classical and molecular genetics." * British Society for the History of Science *"Heredity under the Microscope presents an accurate and sweeping view of the role that cytogenetics played, not only in the history of science after World War II but also as the means by which the current molecular view of the human chromosome was achieved. . . . [de Chadarevian's] is the first story of human cytogenetics told from the historical point of view. . . . The discussions are broad and comprehensive, with careful attention to details. With a few exceptions, they present all the main events occurring in the era of cytogenetics. . . . What is most remarkable to this reviewer, is that the longest section of the book is the notes (67 pp.). This section documents the multitude of sources for what is written in the five chapters. De Chadarevian spoke with a horde of researchers and listened to a great number of interviews with relevant cytogeneticists, and refers to an enormous amount of printed material. Everything in the five chapters is more than adequately supported, and the notes are a delight to read. . . . She deserves our thanks for putting together such a comprehensive survey of human cytogenetics." * FASEB Journal *"A fascinating read on the development of postwar human genetics. . . . This highly readable and impressive book demonstrates the overlapping concerns of science, medicine, law, and policy in the atomic age. De Chadarevian argues that the earlier microscopic research was central to the approach to studying human genetics. This book is a richly sourced survey of human cytogenetics and would be useful for undergraduate teaching." * Journal of the History of Ideas blog * "De Chadarevian's book fully meets its main goal: that of reintroducing chromosomes and cytogenetics into a still simplistic narrative of the history of the understanding of human heredity that traverses the twentieth century. . . . It is a most welcome development in the history of biology." * H-Sci-Med-Tech *"Images of the human chromosomes have enchanted scientists, inspired artists, and become symbols of human difference and pathology. In her engaging account, Soraya de Chadarevian deftly explores the visualization of these cellular 'colored bodies' and demonstrates their critical role in modern biology. The 'molecular revolution,' she suggests, was also a chromosomal revolution." -- M. Susan Lindee, University of Pennsylvania"De Chadarevian shows how much we have missed by looking at the history of biology since World War II as largely a history of how molecular geneticists cracked the genetic code. By focusing the lens on chromosomes, and their uses inside and outside the laboratory, her thorough yet elegant account exposes unexpected continuities, both with the eugenic past of heredity research and its genomic present." -- Staffan Müller-Wille, coauthor of The Gene: From Genetics to Postgenomics"Here we see most vividly how researchers debated the meanings and validity of visual evidence. Heredity Under the Microscope is a remarkable account of what was at stake in visualizing chromosomes." * Journal of the History of Biology *"The book is interesting, informative, and meticulously researched—a must-read for scientists and science studies scholars interested in the history of twentieth-century genetics. Chadarevian’s writing style is accessible and engaging, which also makes the book suitable for nonacademics. The argument, spread over five chapters, is nuanced and compelling." * Isis *Table of ContentsIntroduction 1. Radiation and Mutation 2. Chromosomes and the Clinic 3. X and Y 4. Scaling Up 5. Of Chromosomes and DNA Epilogue Acknowledgments Note on Sources Notes Bibliography Index
£92.15
The University of Chicago Press French DNA Trouble in Purgatory
Book Synopsis"Can a country claim to have its own genetic material?" Rabinow demonstrates that the answer to this question is far from simple. The work is cross-disciplinary, marrying news events, science and philosophical analysis to see how past events influenced current thinking.Trade Review"Can a country claim to have its own genetic material?... Rabinow demonstrates that the answer to this question is far from simple.... The wide variety of subjects that he treats guarantees the interest of a large group of readers: students, researchers, scientific managers from academic institutions as well as from industry, politicians, and interested laymen." - Wilhelm Ansorge, Science "A fast-paced story of personalities and research organizations, interspersed with chapters delving into French history and politics to analyse how past events influenced current thinking and decision making.... The author has done an excellent job of marrying presentation of 'news events,' science, and philosophical analysis, and a book such as this is valuable for its cross-disciplinary insights." - Clare Robinson, Endeavour "Rabinow's book introduces a dramatis personae that could fill the pages of a bio-tech corporate thriller." - Gary Lachman, Times Literary Supplement
£28.00
The University of Chicago Press Evolution and the Genetics of Populations Volum
Book Synopsis"Wright's views about population genetics and evolution are so fundamental and so comprehensive that every serious student must examine these books firsthand. . . . Publication of this treatise is a major event in evolutionary biology."-Daniel L. Hartl, "BioScience"
£47.50
The University of Chicago Press Evolution and the Genetics of Populations
Book Synopsis"Wright's views about population genetics and evolution are so fundamental and so comprehensive that every serious student must examine these books firsthand. . . . Publication of this treatise is a major event in evolutionary biology."-Daniel L. Hartl, "BioScience"
£47.50
The University of Chicago Press Evolution and the Genetics of Populations Volume
Book SynopsisWright's views about population genetics and evolution are so fundamental and so comprehensive that every serious student must examine these books firsthand. . . . Publication of this treatise is a major event in evolutionary biology.-Daniel L. Hartl, BioScience
£52.25
The University of Chicago Press Evolution and the Genetics of Populations Volume
Book Synopsis"Wright's views about population genetics and evolution are so fundamental and so comprehensive that every serious student must examine these books firsthand. . . . Publication of this treatise is a major event in evolutionary biology."-Daniel L. Hartl, "BioScience"
£52.25
Columbia University Press Genetics of Natural Populations
Book SynopsisA discussion of the life and wok of Theodosius Dobzhansky and an assessment of the current research that has the origins in his findings and contributions.
£93.60
Columbia University Press Genes and DNA
Book SynopsisCovers various aspects of contemporary biology including gene therapy, the Human Genome Project, DNA testing, and genetic engineering, and fundamental concepts. This book discusses classical and molecular genetics, quantitative and population genetics including cloning and genetic diseases, and the many applications of genetics to the world.Trade ReviewThe book covers much of the material in a high school textbook...but Omoto and Lurquin write in a way that makes things relevan to any interested adult. I think this is an excellent book that will be of great value in any public library collection...also in university and college libraries. -- Margaret Henderson E-Stream Very useful introduction to genes and genetic applications...Recommended. General readers. -- P. M. Watt Choice As a society we are asked to make informed decisions on complex issues such as stem cell research and the labeling of our food based on its level of genetic modification. We have a lot of homework to do, and this book is a good start. -- Stephen Jones Washington State MagazineTable of ContentsPreface: Why Is Genetics Important? 1. What Are Genes? Try This at Home: Extract DNA from Vegetables in Your Kitchen 2. Inheritance of Single-Gene Traits 3. Mendelian Traits in Humans Try This at Home: Pedigree Game 4. From Genes to Phenotype Try This at Home: DNA Replication, Transcription, and Translation Game 5. Using Bacteria as Protein Factories 6. Genetically Modified Plants 7. When Things Go Wrong 8. Mutagens, Teratogens, and Human Reproduction 9. Linkage and Mapping: Gene Discovery Try This at Home: Independent Assortment of Chromosomes and the Making of a Unique Individual Try This at Home: Explore Genetics Databases 10. Genetics of Populations and Genetic Testing 11. Survival of the Fittest? Try This at Home: Demonstrations of the Effects of Small Population Size 12. Nature Versus Nurture 13. Genetically Modified Animals and the Applications of Gene Technology for Humans Appendix A. Internet Resources Appendix B. Glossary of Scientific Names of Organisms Appendix C. Glossary of Human Genetic Diseases Appendix D. Glossary of Terms
£28.80
Columbia University Press The Theory That Changed Everything
Book SynopsisThe renowned cognitive scientist Philip Lieberman demonstrates that there is no better guide to the world’s living—and still evolving—things than Darwin and that the phenomena he observed are still being explored at the frontiers of science. Lieberman relates the insights that led to groundbreaking discoveries in both Darwin’s time and our own.Trade ReviewLieberman's The Theory That Changed Everything takes Darwin's theory out of academia's dusty lecture halls, returning it to where it began—with a young person curious about the world around them. Engagingly, Lieberman explains how Darwin developed his theories and why those theories matter today. The final chapter, 'What would Darwin Think About…' will energize high school and college biology classes for years to come. -- John J. Shea, Stony Brook UniversityAn awesome accompanying book for anyone who reads On the Origin of Species. -- Rob DeSalle, curator of entomology at the American Museum of Natural HistoryStrikes a balance between the historical context in which Darwin made his remarkable contributions to science and contemporary scientific work. -- Christina Behme, Brandon UniversityLieberman clearly explains complex issues such as epigenetic mechanisms...engage[s] readers interested in the evolution of humans. * Publishers Weekly *An enjoyable and well-written book for those who appreciate Species and its impact on our lives today. * Library Journal *Written with an infectious delight in the way that Darwin’s thinking continues to guide scientific inquiry across disciplines. * Los Angeles Review of Books *Big brains, language, and almost infinite cultural flexibility are quintessential human features. This lucidly written little gem from a leader in the study of human language evolution uses language as a case study to explain how a Darwinian approach is the only way to understand the evolution of human brains and behavior. Written in a very personal voice, it provides a fully accessible synthesis of research in fields as diverse as linguistics, functional genomics, psychology, neuroanatomy, and evolutionary theory. It makes excellent supplementary reading for a diverse range of courses, from evolutionary biology to anthropology and linguistics. -- David Pilbeam, Harvard UniversitySuited for general readers and students interested in the sciences. * Choice *Table of ContentsPrefaceAcknowledgments1. Strawberries2. No Cats, No Flowers3. Grandfather Erasmus4. Crafting the Human Brain5. What Would Darwin Think About . . .NotesBibliographyIndex
£19.00
University of Notre Dame Press Controlling Our Destinies
Book SynopsisThis work considers the complex, far reaching issues surrounding the Human Genome Project - an international scientific enterprise aimed at attaining a complete sequence and locator map of the human genetic structure by the year 2005 - offering the elimination of genetic abnormalities and diseases.Trade Review“The Human Genome Project seeks to pinpoint the genetic basis of virtually any human trait, but it also offers ethical and theological issues which will change human perspective and meaning. Contributors discuss the project’s background, issues, and impact with an eye to revealing the many underlying social changes which will occur as a result of the project.” —Bookwatch“Anyone who is interested in human genetic research will find that this book offers a relevant and interesting analysis of the full range of ethical, philosophical and theological implications of such work. ...[T]he contents of this book are of great importance....” —Addiction Biology“Sloan is absolutely correct in helping his readers see implications well beyond the ethics of medical genetics.… These scientific, historical and philosophical contributions make this volume an invaluable and unique contribution to the growing literature that is exploring the moral and ethical implications of the Human Genome Project....” —National Catholic Bioethics Center“... an innovative contribution to the conversation about human genetics....This is a tremendous book: a unique addition to the scholarly discussion and suited to upper division and graduate biology and humanities courses that seek interdisciplinary inquiry into the implications of human genetic knowledge.” —Religious Studies Review“A fruitful text, the reader is bound to find several essays that meet his or her needs and interests.” —Metapsychology
£25.19
Yale University Press Jacobs Legacy
Book SynopsisWho are the Jews? Where did they come from? What is the connection between an ancient Jewish priest in Jerusalem and today's Israeli sunbather on the beaches of Tel Aviv? This book answers these questions.
£18.57
Yale University Press Ancient DNA
Book SynopsisThe untold story of the rise of a new scientific field, ancient DNA research, and how Jurassic Park and popular media influenced its developmentTrade Review“Fun and thought-provoking. . . . Jones builds a wry, often wise, study of science as a very human endeavor. She makes a powerful case that ancient-DNA research feeds off media attention as much as the media feeds off it.”—Victoria L. Herridge, Nature“Elizabeth D. Jones reveals ancient DNA to be a field of scientific research driven by two forms of contamination—DNA from living organisms and public celebration of the idea of old DNA. She demonstrates the often-underappreciated power of celebrity in driving modern science.”—Beth Shapiro, author of How to Clone a Mammoth and Life As We Made It“A fascinating narrative history of ancient DNA. . . . Elizabeth D. Jones’s insightful arguments and riveting storytelling make this book a pleasure to read.”—Caitlin D. Wylie, author of Preparing Dinosaurs: The Work behind the Scenes“Ancient DNA is a clearly written, fascinating portrait of the development of a high-profile scientific field that was shaped by popular beliefs about DNA and dinosaurs—a wild story of lab results timed to coincide with movie premieres.”—Susan Lindee, Janice and Julian Bers Professor, University of Pennsylvania“Groundbreaking. This book not only explains in careful and clear detail the gradual development of ancient DNA techniques, together with the successes, but also interweaves skillfully the story of how the movie Jurassic Park influenced the science. If you read but one book this year on the making of science, it should be this one.”—Michael Ruse, author of Darwinism as Religion: What Literature Tells Us about Evolution“Ancient DNA fills a major gap in the history of a relatively new science, and in the intersection of modern culture and science communication and practice. I expect it will become very influential and likely will attract the same kind of media attention that its subject generates.”—Dennis O’Rourke, Foundation Distinguished Professor of Anthropology, University of Kansas “Elizabeth Jones’s original contribution to science communication studies richly conceptualizes a novel type of scientific field—a ‘celebrity science,’ one that evolved within the dynamics of publicity, journalism, and popular culture.”—Declan Fahy, author of The New Celebrity Scientists: Out of the Lab and into the Limelight
£28.50
WW Norton & Co MindBrainGene Toward Psychotherapy Integration
Book SynopsisAn exploration of the ways the immune system, epigenetics, affect regulation and attachment intersect in mental health.Trade Review"I did not find this book an easy read but it is a rich and rewarding one. It has both impacted on my understanding of myself and on how I work with others. I cannot recommend it highly enough." -- Transformations Journal
£28.49
John Wiley & Sons Inc Bioinformatics
Book SynopsisBioinformatics is an extremely popular and rapidly growing new discipline that has evolved around the use of algorithmic and computer techniques to analyze large datasets being generated in genomics and related fields. Bioinformatics: Genomics and Post-Genomics provides a clear and concise introduction to the popular new science of bioinformatics.Trade Review"...provides a clear and concise introduction to the popular new science of bioinformatics." (Bioautomation, volume 7)Table of ContentsChapter 1. Genome sequencing. 1.1 Automatic sequencing. 1.2 Sequencing strategies. 1.3 Fragmentation strategies. 1.4 Sequence assembly. 1.5 Filling gaps. 1.6 Obstacles to reconstruction. 1.7 Utilizing a complementary ‘large’ clone library. 1.8 The first large-scale sequencing project: The Haemophilus influenzae genome. 1.9 cDNA and EST. Chapter 2. Sequence comparisons. 2.1 Introduction: Comparison as a sequence prediction method. 2.2 A sample molecule: the human and rosterone receptor. 2.3 Sequence homologies - functional homologies. 2.4 Comparison matrices. 2.5 The problem of insertions and deletions. 2.6 Optimal alignment: the dynamic programming method. 2.7 Fast heuristic methods. 2.8 Sensitivity, specificity, and confidence level. 2.9 Multiple alignments. Chapter 3. Comparative genomics. 3.1 General properties of genomes. 3.2 Genome comparisons. 3.3 Gene evolution and phylogeny: applications to annotation. Chapter 4. Genetic information and biological sequences. 4.1 Introduction: Coding levels. 4.2 Genes and the genetic code. 4.3 Expression signals. 4.4 Specific sites. 4.5 Sites located on DNA. 4.6 Sites present on RNA. 4.7 Pattern detection methods. Chapter 5. Statistics and sequences. 5.1 Introduction. 5.2 Nucleotide base and amino acid distribution. 5.3 The biological basis of codon bias. 5.4 Using statistical bias for prediction. 5.5 Modeling DNA sequences. 5.6 Complex models. 5.7 Sequencing errors and hidden Markov models. 5.8 Hidden Markov processes: a general sequence analysis tool. 5.9 The search for genes - a difficult art. Chapter 6. Structure prediction. 6.1 The structure of RNA. 6.2 Properties of the RNA molecule. 6.3 Secondary RNA structures. 6.4 Thermodynamic stability of RNA structures. 6.5 Finding the most stable structure. 6.6 Validation of predicted secondary structures. 6.7 Using chemical and enzymatic probing to analyze folding. 6.8 Long-distance interactions and three-dimensional structure prediction. 6.9 Protein structure. 6.10 Secondary structure prediction. 6.11 Three-dimensional modeling based on homologous protein structure. 6.12 Predicting folding. Chapter 7. Transcriptome and proteome: macromolecular networks. 7.1 Introduction. 7.2 Post-genomic methods. 7.3 Macromolecular networks. 7.4 Topology of macromolecular networks. 7.5 Modularity and dynamics of macromolecular networks. 7.6 Inference of regulatory networks. Chapter 8. Simulation of Biological Processes in the Genome Context. 8.1 Types of simulations. 8.2 Prediction and explanation. 8.3 Simulation of molecular networks. 8.4 Generic post-genomic simulators. Index.
£62.65
John Wiley & Sons Inc Bioinformatics for Geneticists
Book SynopsisPraise from the reviews: Without reservation, I endorse this text as the best resource I''ve encountered that neatly introduces and summarizes many points I''ve learned through years of experience. The gems of truth found in this book will serve well those who wish to apply bioinformatics in their daily work, as well as help them advise others in this capacity. CIRCGENETICS This book may really help to get geneticists and bioinformaticians on ''speaking-terms''... contains some essential reading for almost any person working in the field of molecular genetics. EUROPEAN JOURNAL OF HUMAN GENETICS ... an excellent resource... this book should ensure that any researcher''s skill base is maintained. GENETICAL RESEARCH one of the best available and most accessible texts on bioinformatics and genetics in the postgenome age The writing is clear, with succinct subsections within each chapter.Without reservation, I endorse this textTrade Review"…provides insights into various areas…" (Books-On-Line)Table of ContentsForeword. Preface. Contributors. Glossary. SECTION I AN INTRODUCTION TO BIOINFORMATICS FOR THE GENETICIST. 1 Bioinformatics challenges for the geneticist (Michael R. Barnes). 1.1 Introduction. 1.2 The role of bioinformatics in genetics research. 1.3 Genetics in the post-genome era. 1.4 Conclusions. References. 2 Managing and manipulating genetic data (Karl W. Broman and Simon C. Heath). 2.1 Introduction. 2.2 Basic principles. 2.3 Data entry and storage. 2.4 Data manipulation. 2.5 Examples of code. 2.6 Resources. 2.7 Summary. References. SECTION II MASTERING GENES, GENOMES AND GENETIC VARIATION DATA. 3 The HapMap – A haplotype map of the human genome (Ellen M. Brown and Bryan J. Barratt). 3.1 Introduction. 3.2 Accessing the data. 3.3 Application of HapMap data in association studies. 3.4 Future Perspectives. References. 4 Assembling a view of the human genome (Colin A. M. Semple). 4.1 Introduction. 4.2 Genomic sequence assembly. 4.3 Annotation from a distance: the generalities. 4.4 Annotation up close and personal: the specifics. 4.5 Annotation: the next generation. References. 5 Finding, delineating and analysing genes (Christopher Southan and Michael R. Barnes). 5.1 Introduction. 5.2 Why learn to predict and analyse genes in the complete genome era? 5.3 The evidence cascade for gene products. 5.4 Dealing with the complexities of gene models. 5.5 Locating known genes in the human genome. 5.6 Genome portal inspection. 5.7 Analysing novel genes. 5.8 Conclusions and prospects. References. 6 Comparative genomics (Martin S. Taylor and Richard R. Copley). 6.1 Introduction. 6.2 The Genomic landscape. 6.3 Concepts. 6.4 Practicalities. 6.5 Technology. 6.6 Applications. 6.7 Challenges and future directions. 6.8 Conclusion. References. SECTION III BIOINFORMATICS FOR GENETIC STUDY DESIGN AND ANALYSIS. 7 Identifying mutations in single gene disorders (David P. Kelsell, Diana Blaydon and Charles A. Mein). 7.1 Introduction. 7.2 Clinical Ascertainment. 7.3 Genome-wide mapping of monogenic diseases. 7.4 The nature of mutation in monogenic diseases. 7.5 Considering epigenetic effects in mendelian traits. 7.6 Summary. References. 8 From Genome Scan Culprit Gene (Ian C. Gray). 8.1 Introduction. 8.2 Theoretical and practical considerations. 8.3 A stepwise approach to locus refinement and candidate gene identification. 8.4 Conclusion. 8.5 A list of the software tools and Web links mentioned in this chapter. References. 9 Integrating Genetics, Genomics and Epigenomics to Identify. Disease Genes (Michael R. Barnes). 9.1 Introduction. 9.2 Dealing with the (draft) human genome sequence. 9.3 Progressing loci of interest with genomic information. 9.4 In silico characterization of the IBD5 locus – a case study. 9.5 Drawing together biological rationale – hypothesis building. 9.6 Identification of potentially functional polymorphisms. 9.7 Conclusions. References. 10 Tools for statistical genetics (Aruna Bansal, Charlotte Vignal and Ralph McGinnis). 10.1 Introduction. 10.2 Linkage analysis. 10.3 Association analysis. 10.4 Linkage disequilibrium. 10.5 Quantitative trait locus (QTL) mapping in experimental crosses. 10.6 Closing remarks. References. SECTION IV MOVING FROM ASSOCIATED GENES TO DISEASE ALLELES. 11 Predictive functional analysis of polymorphisms: An overview (Mary Plumpton and Michael R. Barnes). 11.1 Introduction. 11.2 Principles of predictive functional analysis of polymorphisms. 11.3 The anatomy of promoter regions and regulatory elements. 11.4 The anatomy of genes. 11.5 Pseudogenes and regulatory mRNA. 11.6 Analysis of novel regulatory elements and motifs in. nucleotide sequences. 11.7 Functional analysis of non-synonymous coding polymorphisms. 11.8 Integrated tools for functional analysis of genetic variation. 11.9 A note of caution on the prioritization of in silico predictions for. further laboratory investigation. 11.10 Conclusions. References. 12 Functional in silico analysis of gene regulatory polymorphism (Chaolin Zhang, Xiaoyue Zhao, Michael Q. Zhang). 12.1 Introduction. 12.2 Predicting regulatory regions. 12.3 Modelling and predicting transcription factor-binding sites. 12.4 Predicting regulatory elements for splicing regulation. 12.5 Evaluating the functional importance of. regulatory polymorphisms. References. 13 Amino-acid properties and consequences of substitutions (Matthew J. Betts and Robert B. Russell). 13.1 Introduction. 13.2 Protein features relevant to amino-acid behaviour. 13.3 Amino-acid classifications. 13.4 Properties of the amino acids. 13.5 Amino-acid quick reference. 13.6 Studies of how mutations affect function. 13.7 A summary of the thought process. References. 14 Non-coding RNA bioinformatics (James Brown, Steve Deharo, Barry Dancis, Michael R. Barnes and Philippe Sanseau). 14.1 Introduction. 14.2 The non-coding (nc) RNA universe. 14.3 Computational analysis of ncRNA. 14.4 ncRNA variation in disease. 14.5 Assessing the impact of variation in ncRNA. 14.6 Data resources to support small ncRNA analysis. 14.7 Conclusions. References. SECTION V ANALYSIS AT THE GENETIC AND GENOMIC DATA INTERFACE. 15 What are microarrays? (Catherine A. Ball and Gavin Sherlock). 15.1 Introduction. 15.2 Principles of the application of microarray technology. 15.3 Complementary approaches to microarray analysis. 15.4 Differences between data repository and research database. 15.5 Descriptions of freely available research database packages. References. 16 Combining quantitative trait and gene-expression data (Elissa J. Chesler). 16.1 Introduction: the genetic regulation of endophenotypes. 16.2 Transcript abundance as a complex phenotype. 16.3 Scaling up genetic analysis and mapping models for microarrays. 16.4 Genetic correlation analysis. 16.5 Systems genetic analysis. 16.6 Using expression QTLs to identify candidate genes for the regulation of complex phenotypes. 16.7 Conclusions. References. 17 Bioinformatics and cancer genetics (Joel Greshock). 17.1 Introduction. 17.2 Cancer genomes. 17.3 Approaches to studying cancer genetics. 17.4 General resources for cancer genetics. 17.5 Cancer genes and mutations. 17.6 Copy number alterations in cancer. 17.7 Loss of heterozygosity in cancer. 17.8 Gene-expression data in cancer. 17.9 Multiplatform gene target identification. 17.10 The epigenetics of cancer. 17.11 Tumour modelling. 17.12 Conclusions. References. 18 Needle in a haystack? dealing with 500 SNP genome scans (Michael R. Barnes and Paul S. Derwent). 18.1 Introduction. 18.2 Genome scan analysis issues. 18.3 Ultra-high-density genome-scanning technologies. 18.4 Bioinformatics for genome scan analysis. 18.5 Conclusions. References. 19 A bioinformatics perspective on genetics in drug discovery and development (Christopher D. Southan, Magnus Ulvsb¨ack and Michael R. Barnes). 19.1 Introduction. 19.2 Target genetics. 19.3 Pharmacogenetics (PGx). 19.4 Conclusions: toward ‘personalized medicine’. References. Appendix I. Appendix II. Index.
£170.06
John Wiley & Sons Inc Statistical DNA Forensics
Book SynopsisStatistical methodology plays a key role in ensuring that DNA evidence is collected, interpreted, analyzed and presented correctly. With the recent advances in computer technology, this methodology is more complex than ever before. There are a growing number of books in the area but none are devoted to the computational analysis of evidence. This book presents the methodology of statistical DNA forensics with an emphasis on the use of computational techniques to analyze and interpret forensic evidence.Table of ContentsPreface. List of figures. List of tables. 1. Introduction. 1.1 Statistics, forensic science and the law. 1.2 The use of statistics in forensic DNA. 1.3 Genetic basis of DNA profiling and typing technology. 1.3.1 Genetic basis. 1.3.2 Typing technology. 1.4 About the book. 2. Probability and statistics. 2.1 Probability. 2.2 Dependent events and conditional probability. 2.3 Law of total probability. 2.4 Bayes’ Theorem. 2.5 Binomial probability distribution. 2.6 Multinomial distribution. 2.7 Poisson distribution. 2.8 Normal distribution. 2.9 Likelihood ratio. 2.10 Statistical inference. 2.10.1 Test of hypothesis. 2.10.2 Estimation and testing. 2.11 Problems. 3. Population genetics. 3.1 Hardy-Weinberg equilibrium. 3.2 Test for Hardy-Weinberg equilibrium. 3.2.1 Observed and expected heterozygosities. 3.2.2 Chi-square test. 3.2.3 Fisher’s exact test. 3.2.4 Computer software. 3.3 Other statistics for analysis of a population database. 3.3.1 Linkage equilibrium. 3.3.2 Power of discrimination. 3.4 DNA profiling. 3.5 Subpopulation models. 3.6 Relatives. 3.7 Problems. 4. Parentage testing. 4.1 Standard trio. 4.1.1 Paternity index. 4.1.2 An example. 4.1.3 Posterior odds and probability of paternity. 4.2 Paternity computer software. 4.2.1 Steps in running the software. 4.2.2 The software to deal with an incest case. 4.3 A relative of the alleged father is the true father. 4.4 Alleged father unavailable but his relative is. 4.5 Motherless case. 4.5.1 Paternity index. 4.5.2 Computer software and example. 4.6 Motherless case: relatives involved. 4.6.1 A relative of the alleged father is the true father. 4.6.2 Alleged father unavailable but his relative is. 4.6.3 Computer software and example. 4.7 Determination of both parents. 4.8 Probability of excluding a random man from paternity. 4.9 Power of exclusion. 4.9.1 A random man case. 4.9.2 A relative case. 4.9.3 An elder brother case: mother available. 4.10 Other issues. 4.10.1 Reverse parentage. 4.10.2 Mutation. 4.11 Problems. 5. Testing for kinship. 5.1 Kinship testing of any two persons: HWE. 5.2 Computer software. 5.3 Kinship testing of two persons: subdivided populations. 5.3.1 Joint genotype probability. 5.3.2 Relatives involved. 5.4 Examples with software. 5.5 Three persons situation: HWE. 5.6 Computer software and example. 5.7 Three persons situation: subdivided populations. 5.7.1 Standard trio. 5.7.2 A relative of the alleged father is the true father. 5.7.3 Alleged father unavailable but his relative is. 5.7.4 Example. 5.7.5 General method and computer software. 5.8 Complex kinship determinations: method and software. 5.8.1 EasyPA_In_1_Minute software and the method. 5.8.2 EasyPAnt_In_1_Minute. 5.8.3 EasyIN_In_1_Minute. 5.8.4 EasyMISS_In_1_Minute. 5.8.5 Other considerations: probability of paternity and mutation. 5.9 Problems. 6. Interpreting mixtures. 6.1 An illustrative example. 6.2 Some common cases and a case example. 6.2.1 One victim, one suspect and one unknown. 6.2.2 One suspect and two unknowns. 6.2.3 Two suspects and two unknowns. 6.2.4 Case example. 6.2.5 Exclusion probability. 6.3 A general approach. 6.4 Population in Hardy-Weinberg equilibrium. 6.5 Population with multiple ethnic groups. 6.6 Subdivided population. 6.6.1 Single ethnic group: simple cases. 6.6.2 Single ethnic group: general situations. 6.6.3 Multiple ethnic groups. 6.7 Computer software and example. 6.8 NRC II Recommendation 4.1. 6.8.1 Single ethnic group. 6.8.2 Multiple ethnic groups. 6.9 Proofs. 6.9.1 The proof of Equation (6.6). 6.9.2 The proof of Equation (6.8). 6.9.3 The proof of Equation (6.9). 6.9.4 The proofs of Equations (6.11) and (6.12). 6.9.5 The proofs of Equations (6.14) and (6.15). 6.10 Problems. 7. Interpreting mixtures in the presence of relatives. 7.1 One pair of relatives: HWE. 7.1.1 Motivating example. 7.1.2 A probability formula. 7.1.3 Tested suspect with an unknown relative. 7.1.4 Unknown suspect with a tested relative. 7.1.5 Two related persons were unknown contributors. 7.1.6 An application. 7.2 Two pairs of relatives: HWE. 7.2.1 Two unknowns related respectively to two typed persons. 7.2.2 One unknown is related to a typed person and two other. unknowns are related. 7.2.3 Two pairs of related unknowns. 7.2.4 Examples. 7.2.5 Extension. 7.3 Related people from the same subdivided population. 7.3.1 Introductory example. 7.3.2 A simple case with one victim, one suspect and one relative. 7.3.3 General formulas. 7.3.4 An example analyzed by the software. 7.4 Proofs. 7.4.1 Preliminary. 7.4.2 The proof of Equation (7.5). 7.4.3 The proof of Equation (7.7). 7.4.4 The proof of Equation (7.9). 7.4.5 The proof of Equation (7.11). 7.4.6 The proof of Equation (7.13). 7.4.7 The proofs of Equations (7.18) and (7.20). 7.5 Problems. 8. Other issues. 8.1 Lineage markers. 8.2 Haplotypic genetic markers for mixture. 8.3 Bayesian network. 8.4 Peak information. 8.5 Mass disaster. 8.6 Database search. Solutions to Problems. Appendix A: The standard normal distribution. Appendix B: Upper 1% and 5% points of w2 distributions. Bibliography. Index.
£83.66
John Wiley and Sons Ltd Human Population Genetics
Book SynopsisHuman Population Genetics will provide an introduction to mathematical population genetics, along with relevant examples from human (and some non-human primate) populations, and will also present concepts and methods of population genetics that are specific to the study of human populations.Trade Review“Relethford’s Human Population Geneticsis a superb attempt at facing the challenges of explaining the basics of population genetics to those with a limited background in evolutionary theory and a fear of the quantitative.” (The Quarterly Review of Biology, 1 September 2014) “For many students, and likely some instructors, who have found the mathematical underpinnings of evolutionary genetics daunting, this new volume will be a welcome addition to the bookshelf. It is an easy book to recommend either as a primary text in anthropological genetics courses, or as a recommended or adjunct text in upper division/beginning graduate courses in human biology, human genetics, or human evolution.” (American Journal of Physical Anthropology, 19 September 2013) Table of ContentsForeword vii Preface ix 1 Genetic, Mathematical, and Anthropological Background 1 I. The Scope of Population Genetics 2 II. Genetics Background 5 III. Principles of Probability 14 IV. The Anthropological Connection 17 V. A Closing Thought 21 2 Hardy–Weinberg Equilibrium 23 I. Genotype and Allele Frequencies 24 II. What is Hardy–Weinberg Equilibrium? 30 III. The Mathematics of Hardy–Weinberg Equilibrium 31 IV. Using Hardy–Weinberg Equilibrium 37 V. Extensions of Hardy–Weinberg Equilibrium 40 VI. Hardy–Weinberg Equilibrium and Evolution 44 VII. Summary 45 3 Inbreeding 49 I. Quantifying Inbreeding 51 II. Population Genetics and Inbreeding 62 III. Inbreeding in Human Populations 65 IV. Summary 75 4 Mutation 77 I. The Nature of Mutations 77 II. Models of Mutation 81 III. Mutational History and Anthropological Questions 88 IV. Summary 96 5 Genetic Drift 101 I. What is Genetic Drift? 102 II. Genetic Drift and Population Size 112 III. Effects on Genetic Variation 120 IV. Mutation and Genetic Drift 121 V. Coalescent Theory 125 VI. Summary 131 6 Models of Natural Selection 139 I. How Does Natural Selection Work? 140 II. A General Model of Natural Selection 145 III. Types of Natural Selection 147 IV. Other Aspects of Selection 160 V. Summary 167 7 Natural Selection in Human Populations 181 I. Case Studies of Natural Selection in Human Populations 182 II. Are Humans Still EvolVIng? 198 III. Summary 203 8 Gene Flow 205 I. The Evolutionary Impact of Gene Flow 206 II. Models of Gene Flow 208 III. Gene Flow and Genetic Drift 213 IV. Estimating Admixture in Human Populations 226 V. Summary 230 9 Human Population Structure and History 237 I. Case Studies of Human Population Structure 238 II. The Origin of Modern Humans 242 III. Case Studies of Population Origins 247 IV. Summary 255 Glossary 257 References 267 Index 279
£62.96
John Wiley and Sons Ltd ForwardTime Population Genetics
Book SynopsisThe only book available in the area of forward-time population genetics simulationsapplicable to both biomedical and evolutionary studies The rapid increase of the power of personal computers has led to the use of serious forward-time simulation programs in genetic studies. Forward-Time Population Genetics Simulations presents both new and commonly used methods, and introduces simuPOP, a powerful and flexible new program that can be used to simulate arbitrary evolutionary processes with unique features like customized chromosome types, arbitrary nonrandom mating schemes, virtual subpopulations, information fields, and Python operators. The book begins with an overview of important concepts and models, then goes on to show how simuPOP can simulate a number of standard population genetics modelswith the goal of demonstrating the impact of genetic factors such as mutation, selection, and recombination on standard Wright-Fisher models. The rest of the book is devoted to aTable of ContentsPreface ix Acknowledgments xiii List of examples xxiii 1. Basic concepts and models 1 1.1 Biological and genetic concepts 2 1.2 Population and evolutionary genetics 6 1.3 Statistical genetics and genetic epidemiology 17 2. Simulation of population genetics models 25 2.1 Random genetic drift 25 2.2 Demographic models 29 2.3 Mutation 31 2.4 Migration 34 2.5 Recombination and linkage disequilibrium 36 2.6 Natural selection 37 2.7 Genealogy of forward-time simulations 41 3. Ascertainment bias in population genetics 47 3.1 Introduction 47 3.2 Methods 49 3.3 Results 54 3.4 Discussion and Conclusions 58 4. Observing properties of evolving populations 63 4.1 Introduction 64 4.2 Simulation of the evolution of allele spectra 66 4.3 Extensions to the basic model 78 5. Simulating populations with complex human diseases 89 5.1 Introduction 89 5.2 Controlling disease allele frequencies at the present generation 91 5.3 Forward-time simulation of realistic samples 102 5.4 Discussion 119 6. Nonrandom mating and its applications 125 6.1 Assortative mating 126 6.2 More complex non-random mating schemes 132 6.3 Hetergeneous mating schemes 140 6.4 Simulation of age structured populations 145 Appendix: Forward-time simulations using stimulPOP 157 A.1 Introduction 157 A.2 Population 160 A.3 Operators 172 A.4 Evolve on or more populations 181 A.5 A complete stimuPOP script 185
£86.36
John Wiley & Sons Inc From Genes to Genomes
Book SynopsisThe latest edition of this highly successful textbook introduces the key techniques and concepts involved in cloning genes and in studying their expression and variation. The new edition features: Increased coverage of whole-genome sequencing technologies and enhanced treatment of bioinformatics. Clear, two-colour diagrams throughout. A dedicated website including all figures. Noted for its outstanding balance between clarity of coverage and level of detail, this book provides an excellent introduction to the fast moving world of molecular genetics.Trade Review“This third edition is absolutely necessary to incorporate the recent advances, such as genome sequencing, polymerase chain reaction, and microarray technology, in this field.” (Doody’s, 19 October 2012)Table of ContentsPreface xiii 1 From Genes to Genomes 1 1.1 Introduction 1 1.2 Basic molecular biology 4 1.2.1 The DNA backbone 4 1.2.2 The base pairs 6 1.2.3 RNA structure 10 1.2.4 Nucleic acid synthesis 11 1.2.5 Coiling and supercoilin 11 1.3 What is a gene? 13 1.4 Information flow: gene expression 15 1.4.1 Transcription 16 1.4.2 Translation 19 1.5 Gene structure and organisation 20 1.5.1 Operons 20 1.5.2 Exons and introns 21 1.6 Refinements of the model 22 2 How to Clone a Gene 25 2.1 What is cloning? 25 2.2 Overview of the procedures 26 2.3 Extraction and purification of nucleic acids 29 2.3.1 Breaking up cells and tissues 29 2.3.2 Alkaline denaturation 31 2.3.3 Column purification 31 2.4 Detection and quantitation of nucleic acids 32 2.5 Gel electrophoresis 33 2.5.1 Analytical gel electrophoresis 33 2.5.2 Preparative gel electrophoresis 36 2.6 Restriction endonucleases 36 2.6.1 Specificity 37 2.6.2 Sticky and blunt ends 40 2.7 Ligation 42 2.7.1 Optimising ligation conditions 44 2.7.2 Preventing unwanted ligation: alkaline phosphatase and double digests 46 2.7.3 Other ways of joining DNA fragments 48 2.8 Modification of restriction fragment ends 49 2.8.1 Linkers and adaptors 50 2.8.2 Homopolymer tailing 52 2.9 Plasmid vectors 53 2.9.1 Plasmid replication 54 2.9.2 Cloning sites 55 2.9.3 Selectable markers 57 2.9.4 Insertional inactivation 58 2.9.5 Transformation 59 2.10 Vectors based on the lambda bacteriophage 61 2.10.1 Lambda biology 61 2.10.2 In vitro packaging 65 2.10.3 Insertion vectors 66 2.10.4 Replacement vectors 68 2.11 Cosmids 71 2.12 Supervectors: YACs and BACs 72 2.13 Summary 73 3 Genomic and cDNA Libraries 75 3.1 Genomic libraries 77 3.1.1 Partial digests 77 3.1.2 Choice of vectors 80 3.1.3 Construction and evaluation of a genomic library 83 3.2 Growing and storing libraries 86 3.3 cDNA libraries 87 3.3.1 Isolation of mRNA 88 3.3.2 cDNA synthesis 89 3.3.3 Bacterial cDNA 93 3.4 Screening libraries with gene probes 94 3.4.1 Hybridization 94 3.4.2 Labelling probes 98 3.4.3 Steps in a hybridization experiment 99 3.4.4 Screening procedure 100 3.4.5 Probe selection and generation 101 3.5 Screening expression libraries with antibodies 103 3.6 Characterization of plasmid clones 106 3.6.1 Southern blots 107 3.6.2 PCR and sequence analysis 108 4 Polymerase Chain Reaction (PCR) 109 4.1 The PCR reaction 110 4.2 PCR in practice 114 4.2.1 Optimisation of the PCR reaction 114 4.2.2 Primer design 115 4.2.3 Analysis of PCR products 117 4.2.4 Contamination 118 4.3 Cloning PCR products 119 4.4 Long-range PCR 121 4.5 Reverse-transcription PCR 123 4.6 Quantitative and real-time PCR 123 4.6.1 SYBR Green 123 4.6.2 TaqMan 125 4.6.3 Molecular beacons 125 4.7 Applications of PCR 127 4.7.1 Probes and other modified products 127 4.7.2 PCR cloning strategies 128 4.7.3 Analysis of recombinant clones and rare events 129 4.7.4 Diagnostic applications 130 5 Sequencing a Cloned Gene 131 5.1 DNA sequencing 131 5.1.1 Principles of DNA sequencing 131 5.1.2 Automated sequencing 136 5.1.3 Extending the sequence 137 5.1.4 Shotgun sequencing; contig assembly 138 5.2 Databank entries and annotation 140 5.3 Sequence analysis 146 5.3.1 Identification of coding region 146 5.3.2 Expression signals 147 5.4 Sequence comparisons 148 5.4.1 DNA sequences 148 5.4.2 Protein sequence comparisons 151 5.4.3 Sequence alignments: Clustal 157 5.5 Protein structure 160 5.5.1 Structure predictions 160 5.5.2 Protein motifs and domains 162 5.6 Confirming gene function 165 5.6.1 Allelic replacement and gene knockouts 166 5.6.2 Complementation 168 6 Analysis of Gene Expression 169 6.1 Analysing transcription 169 6.1.1 Northern blots 170 6.1.2 Reverse transcription-PCR 171 6.1.3 In situ hybridization 174 6.2 Methods for studying the promoter 174 6.2.1 Locating the promoter 175 6.2.2 Reporter genes 177 6.3 Regulatory elements and DNA-binding proteins 179 6.3.1 Yeast one-hybrid assays 179 6.3.2 DNase I footprinting 181 6.3.3 Gel retardation assays 181 6.3.4 Chromatin immunoprecipitation (ChIP) 183 6.4 Translational analysis 185 6.4.1 Western blots 185 6.4.2 Immunocytochemistry and immunohistochemistry 187 7 Products from Native and Manipulated Cloned Genes 189 7.1 Factors affecting expression of cloned genes 190 7.1.1 Transcription 190 7.1.2 Translation initiation 192 7.1.3 Codon usage 193 7.1.4 Nature of the protein product 194 7.2 Expression of cloned genes in bacteria 195 7.2.1 Transcriptional fusions 195 7.2.2 Stability: conditional expression 198 7.2.3 Expression of lethal genes 201 7.2.4 Translational fusions 201 7.3 Yeast systems 204 7.3.1 Cloning vectors for yeasts 204 7.3.2 Yeast expression systems 206 7.4 Expression in insect cells: baculovirus systems 208 7.5 Mammalian cells 209 7.5.1 Cloning vectors for mammalian cells 210 7.5.2 Expression in mammalian cells 213 7.6 Adding tags and signals 215 7.6.1 Tagged proteins 215 7.6.2 Secretion signals 217 7.7 In vitro mutagenesis 218 7.7.1 Site-directed mutagenesis 218 7.7.2 Synthetic genes 223 7.7.3 Assembly PCR 223 7.7.4 Synthetic genomes 224 7.7.5 Protein engineering 224 7.8 Vaccines 225 7.8.1 Subunit vaccines 225 7.8.2 DNA vaccines 226 8 Genomic Analysis 229 8.1 Overview of genome sequencing 229 8.1.1 Strategies 230 8.2 Next generation sequencing (NGS) 231 8.2.1 Pyrosequencing (454) 232 8.2.2 SOLiD sequencing (Applied Biosystems) 235 8.2.3 Bridge amplification sequencing (Solexa/Ilumina) 237 8.2.4 Other technologies 239 8.3 De novo sequence assembly 239 8.3.1 Repetitive elements and gaps 240 8.4 Analysis and annotation 242 8.4.1 Identification of ORFs 243 8.4.2 Identification of the function of genes and their products 250 8.4.3 Other features of nucleic acid sequences 251 8.5 Comparing genomes 256 8.5.1 BLAST 256 8.5.2 Synteny 257 8.6 Genome browsers 258 8.7 Relating genes and functions: genetic and physical maps 260 8.7.1 Linkage analysis 261 8.7.2 Ordered libraries and chromosome walking 262 8.8 Transposon mutagenesis and other screening techniques 263 8.8.1 Transposition in bacteria 263 8.8.2 Transposition in Drosophila 266 8.8.3 Transposition in other organisms 268 8.8.4 Signature-tagged mutagenesis 269 8.9 Gene knockouts, gene knockdowns and gene silencing 271 8.10 Metagenomics 273 8.11 Conclusion 274 9 Analysis of Genetic Variation 275 9.1 Single nucleotide polymorphisms 276 9.1.1 Direct sequencing 278 9.1.2 SNP arrays 279 9.2 Larger scale variations 280 9.2.1 Microarrays and indels 281 9.3 Other methods for studying variation 282 9.3.1 Genomic Southern blot analysis: restriction fragment length polymorphisms (RFLPs) 282 9.3.2 VNTR and microsatellites 285 9.3.3 Pulsed-field gel electrophoresis 287 9.4 Human genetic variation: relating phenotype to genotype 289 9.4.1 Linkage analysis 289 9.4.2 Genome-wide association studies (GWAS) 292 9.4.3 Database resources 294 9.4.4 Genetic diagnosis 294 9.5 Molecular phylogeny 295 9.5.1 Methods for constructing trees 298 10 Post-Genomic Analysis 305 10.1 Analysing transcription: transcriptomes 305 10.1.1 Differential screening 306 10.1.2 Other methods: transposons and reporters 308 10.2 Array-based methods 308 10.2.1 Expressed sequence tag (EST) arrays 309 10.2.2 PCR product arrays 310 10.2.3 Synthetic oligonucleotide arrays 312 10.2.4 Important factors in array hybridization 313 10.3 Transcriptome sequencing 315 10.4 Translational analysis: proteomics 316 10.4.1 Two-dimensional electrophoresis 317 10.4.2 Mass spectrometry 318 10.5 Post-translational analysis: protein interactions 320 10.5.1 Two-hybrid screening 320 10.5.2 Phage display libraries 321 10.6 Epigenetics 323 10.7 Integrative studies: systems biology 324 10.7.1 Metabolomic analysis 324 10.7.2 Pathway analysis and systems biology 325 11 Modifying Organisms: Transgenics 327 11.1 Transgenesis and cloning 327 11.1.1 Common species used for transgenesis 328 11.1.2 Control of transgene expression 330 11.2 Animal transgenesis 333 11.2.1 Basic methods 333 11.2.2 Direct injection 333 11.2.3 Retroviral vectors 335 11.2.4 Embryonic stem cell technology 336 11.2.5 Gene knockouts 339 11.2.6 Gene knock-down technology: RNA interference 340 11.2.7 Gene knock-in technology 341 11.3 Applications of transgenic animals 342 11.4 Disease prevention and treatment 343 11.4.1 Live vaccine production: modification of bacteria and viruses 343 11.4.2 Gene therapy 346 11.4.3 Viral vectors for gene therapy 347 11.5 Transgenic plants and their applications 349 11.5.1 Introducing foreign genes 349 11.5.2 Gene subtraction 351 11.5.3 Applications 352 11.6 Transgenics: a coda 353 Glossary 355 Bibliography 375 Index 379
£108.86
John Wiley & Sons Inc Protein Bioinformatics An Algorithmic Approach to
Book SynopsisThis book takes the novel approach to cover both the sequence and structure analysis of proteins in one volume and from an algorithmic perspective. Key features of the book include:* Provides a comprehensive introduction to the analysis of protein sequence and structure analysis.Table of ContentsPreface. Acknowledgements. Part I: SEQUENCE ANALYSIS. 1. Pairwise Global Alignment of Sequences. 1.1 Alignment and Evolution. 1.2 What is an Alignment? 1.3 A Scoring Scheme for the Model. 1.4 Finding Highest-Scoring Alignments with Dynamic Programming. 1.4.1 Determine Hi,j. 1.4.2 Use of matrices. 1.4.3 Finding the alignments that give the highest score. 1.4.4 Gaps. 1.5 Scoring Matrices. 1.6 Scoring Gaps: Gap Penalties. 1.7 Dynamic Programming for General Gap Penalty. 1.8 Dynamic Programming for Affine Gap Penalty. 1.9 Alignment Score and Sequence Distance. 1.10 Exercises. 1.11 Bibliographic notes. 2 Pairwise Local Alignment and Database Search. 2.1 The Basic Operation: Comparing Two Sequences. 2.2 Dot Matrices. 2.2.1 Filtering. 2.2.2 Repeating segments. 2.3 Dynamic Programming. 2.3.1 Initialization. 2.3.2 Finding the best local alignments. 2.3.3 Algorithms. 2.3.4 Scoring matrices and gap penalties. 2.4 Database Search: BLAST. 2.4.1 The procedure. 2.4.2 Preprocess the query: make the word list. 2.4.3 Scanning the database sequences. 2.4.4 Extending to HSP. 2.4.5 Introducing gaps. 2.4.6 Algorithm. 2.5 Exercises. 2.6 Bibliographic notes. 3. Statistical Analysis. 3.1 Hypothesis Testing for Sequence Homology. 3.1.1 Random generation of sequences. 3.1.2 Use of Z values for estimating the statistical significance. 3.2 Statistical Distributions. 3.2.1 Poisson probability distribution. 3.2.2 Extreme value distributions. 3.3 Theoretical Analysis of Statistical Significance. 3.3.1 The P value has an extreme value distribution. 3.3.2 Theoretical analysis for database search. 3.4 Probability Distributions for Gapped Alignments. 3.5 Assessing and Comparing Programs for Database Search. 3.5.1 Sensitivity and specificity. 3.5.2 Discrimination power. 3.5.3 Using more sequences as queries. 3.6 Exercises. 3.7 Bibliographic notes. 4 .Multiple Global Alignment and Phylogenetic Trees. 4.1 Dynamic Programming. 4.1.1 SP score of multiple alignments. 4.1.2 A pruning algorithm for the DP solution. 4.2 Multiple Alignments and Phylogenetic Trees. 4.3 Phylogeny. 4.3.1 The number of different tree topologies. 4.3.2 Molecular clock theory. 4.3.3 Additive and ultrametric trees. 4.3.4 Different approaches for reconstructing phylogenetic trees. 4.3.5 Distance-based construction. 4.3.6 Rooting of trees. 4.3.7 Statistical test: bootstrapping. 4.4 Progressive Alignment. 4.4.1 Aligning two subset alignments. 4.4.2 Clustering. 4.4.3 Sequence weights. 4.4.4 CLUSTAL. 4.5 Other Approaches. 4.6 Exercises. 4.7 Bibliographic notes. 5. Scoring Matrices. 5.1 Scoring Matrices Based on Physio-Chemical Properties. 5.2 PAM Scoring Matrices. 5.2.1 The evolutionary model. 5.2.2 Calculate substitution matrix. 5.2.3 Matrices for general evolutionary time. 5.2.4 Measuring sequence similarity by use of Mτ. 5.2.5 Odds matrices. 5.2.6 Scoring matrices (log-odds matrices). 5.2.7 Estimating the evolutionary distance. 5.3 BLOSUM Scoring Matrices. 5.3.1 Log-odds matrix. 5.3.2 Developing scoring matrices for different evolutionary distances. 5.4 Comparing BLOSUM and PAM Matrices. 5.5 Optimal Scoring Matrices. 5.5.1 Analysis for one sequence. 5.6 Exercises. 5.7 Bibliographic notes. 6. Profiles. 6.1 Constructing a Profile. 6.1.1 Notation. 6.1.2 Removing rows and columns. 6.1.3 Position weights. 6.1.4 Sequence weights. 6.1.5 Treating gaps. 6.2 Searching Databases with Profiles. 6.3 Iterated BLAST: PSI-BLAST. 6.3.1 Making the multiple alignment. 6.3.2 Constructing the profile. 6.4 HMM Profile. 6.4.1 Definitions for an HMM. 6.4.2 Constructing a profile HMM for a protein family. 6.4.3 Comparing a sequence with an HMM. 6.4.4 Protein family databases. 6.5 Exercises. 6.6 Bibliographic notes. 7. Sequence Patterns. 7.1 The PROSITE Language. 7.2 Exact/Approximate Matching. 7.3 Defining Pattern Classes by Imposing Constraints. 7.4 Pattern Scoring: Information Theory. 7.4.1 Information theory. 7.4.2 Scoring patterns. 7.5 Generalization and Specialization. 7.6 Pattern Discovery: Introduction. 7.7 Comparison-Based Methods. 7.7.1 Pivot-based methods. 7.7.2 Tree progressive methods. 7.8 Pattern-Driven Methods: Pratt. 7.8.1 The main procedure. 7.8.2 Preprocessing. 7.8.3 The pattern space. 7.8.4 Searching. 7.8.5 Ambiguous components. 7.8.6 Specialization. 7.8.7 Pattern scoring. 7.9 Exercises. 7.10 Bibliographic notes. Part II: STRUCTURE ANALYSIS 8. Structures and Structure Descriptions. 8.1 Units of Structure Descriptions. 8.2 Coordinates. 8.3 Distance Matrices. 8.4 Torsion Angles. 8.5 Coarse Level Description. 8.5.1 Line segments (sticks). 8.5.2 Ellipsoid. 8.5.3 Helices. 8.5.4 Strands and sheets. 8.5.5 Topology of Protein Structure (TOPS). 8.6 Identifying the SSEs. 8.6.1 Use of distance matrices. 8.6.2 Define Secondary Structure of Proteins (DSSP). 8.7 Structure Comparison. 8.7.1 Structure descriptions for comparison. 8.7.2 Structure representation. 8.8 Framework for Pairwise Structure Comparison. 8.9 Exercises. 8.10 Bibliographic notes. 9. Superposition and Dynamic Programming. 9.1 Superposition. 9.1.1 Coordinate RMSD. 9.1.2 Distance RMSD. 9.1.3 Using RMSD as scoring of structure similarities. 9.2 Alternating Superposition and Alignment. 9.3 Double Dynamic Programming. 9.3.1 Low-level scoring matrices. 9.3.2 High-level scoring matrix. 9.3.3 Iterated double dynamic programming. 9.4 Similarity of the Methods. 9.5 Exercises. 9.6 Bibliographic notes. 10. Geometric Techniques. 10.1 Geometric Hashing. 10.1.1 Two-dimensional geometric hashing. 10.1.2 Geometric hashing for structure comparison. 10.1.3 Geometric hashing for SSE representation. 10.1.4 Clustering. 10.2 Distance Matrices. 10.2.1 Measuring the similarity of distance (sub)matrices. 10.3 Exercises. 10.4 Bibliographic notes. 11. Clustering: Combining Local Similarities. 11.1 Compatibility and Consistency. 11.2 Searching for Seed Matches. 11.3 Consistency. 11.3.1 Test for consistency. 11.3.2 Overlapping clusters. 11.4 Clustering Algorithms. 11.4.1 Linear clustering. 11.4.2 Hierarchical clustering. 11.5 Clustering by Use of Transformations. 11.5.1 Comparing transformations. 11.5.2 Calculating the new transformation. 11.5.3 Algorithm. 11.6 Clustering by Use of Relations. 11.6.1 How many relations to compare? 11.6.2 Geometric relation. 11.6.3 Distance relation. 11.6.4 Use of graph theory. 11.7 Refinement. 11.8 Exercises. 11.9 Bibliographic notes. 12. Significance and Assessment of Structure Comparisons. 12.1 Constructing Random Structure Models. 12.1.1 Use of distance geometry. 12.2 Use of Structure Databases. 12.2.1 Constructing nonredundant subsets. 12.2.2 Demarcation line for similarity. 12.3 Reversing the Protein Chain. 12.4 Randomized Alignment Models. 12.5 Assessing Comparison and Scoring Methods. 12.6 Is RMSD Suitable for Scoring? 12.7 Scoring and Biological Significance. 12.8 Exercises. 12.9 Bibliographic notes. 13. Multiple Structure Comparison. 13.1 Multiple Superposition. 13.2 Progressive Structure Alignment. 13.2.1 Scoring. 13.2.2 Construction of consensus. 13.3 Finding a Common Core from a Multiple Alignment. 13.4 Discovering Common Cores. 13.4.1 Finding the multiple seed matches. 13.4.2 Pairwise clustering. 13.4.3 Determining common cores. 13.4.4 Scoring clusters. 13.5 Local Structure Patterns. 13.5.1 Local packing patterns. 13.5.2 Discovering packing patterns. 13.5.3 The approach. 13.5.4 Scoring the packing motifs. 13.6 Exercises. 13.7 Bibliographic notes. 14. Protein Structure Classification. 14.1 Protein Domains. 14.2 An Ising Model for Domain Identification. 14.3 Domain Classes. 14.3.1 Mainly-? domains. 14.3.2 Mainly-? domains. 14.3.3 ?–? domains. 14.4 Folds. 14.5 Automatic Approaches to Classification. 14.6 Databases for Structure Classification. 14.7 FSSP-Dali Domain Dictionary. 14.8 CATH. 14.8.1 Domains. 14.8.2 Class. 14.8.3 Architecture. 14.8.4 Topology (fold family). 14.8.5 Homologous superfamily. 14.8.6 Sequence families. 14.8.7 The CATH classification procedure. 14.9 Classification Based on Sticks. 14.10 Exercises. 14.11 Bibliographic notes. Part III: SEQUENCE-STRUCTUREANALYSIS. 15. Structure Prediction: Threading. 15.1 Protein Secondary Structure Prediction. 15.1.1 Artificial neural networks. 15.1.2 The PHD program. 15.1.3 Accuracy in secondary structure prediction. 15.2 Threading. 15.3 Methods Based on Sequence Alignment. 15.3.1 The 3D–1D matching method. 15.3.2 The FUGUE method. 15.4 Methods Using 3D Interactions. 15.4.1 Potentials of mean force. 15.4.2 Towards modelling methods. 15.5 Alignment Methods. 15.5.1 Frozen approximation. 15.5.2 Double Dynamic Programming. 15.6 Multiple Sequence/Structure Threading. 15.6.1 Simple multiple sequence threading. 15.7 Combined Sequence/Threading Methods. 15.8 Assessment of Threading Methods. 15.8.1 Fold recognition. 15.8.2 Alignment accuracy. 15.8.3 CASP and CAFASP. 15.9 Bibliographic notes. Appendix A: Basics in Mathematics, Probability and Algorithms. A.1 Mathematical Formulae and Notation. A.2 Boolean Algebra. A.3 Set Theory. A.4 Probability. A.4.1 Permutation and combination. A.4.2 Probability distributions. A.4.3 Expected value. A.5 Tables, Vectors and Matrices. A.6 Algorithmic Language. A.6.1 Alternatives. A.6.2 Loops. A.7 Complexity. Appendix B: Introduction to Molecular Biology. B.1 The Cell and the Molecules of Life: DNA–RNA Proteins. B.2 Chromosomes and Genes. B.3 The Central Dogma of Molecular Biology. B.4 The Genetic Code. B.5 Protein Function. B.5.1 The gene ontology. B.6 Protein Structure. B.7 Evolution. B.8 Insulin Example. B.9 Bibliographic notes. References. Index.
£69.26
John Wiley and Sons Ltd Seed Genomics
Book SynopsisThis up-to-date review of seed genomics, from basic seed biology to practical applications in crop science, provides a thorough background understanding of seed biology from a basic science perspective.Table of ContentsContributors xi Introduction 1 Philip W. Becraft Chapter 1 Large-Scale Mutant Analysis of Seed Development in Arabidopsis 5 David W. Meinke Introduction 5 Historical Perspective 5 Arabidopsis Embryo Mutant System 7 Large-Scale Forward Genetic Screens for Seed Mutants 7 Approaches to Mutant Analysis 8 Strategies for Approaching Saturation 10 SeedGenes Database of Essential Genes in Arabidopsis 11 Embryo Mutants with Gametophyte Defects 13 General Features of EMB Genes in Arabidopsis 14 Value of Large Datasets of Essential Genes 15 Directions for Future Research 16 Acknowledgments 17 References 17 Chapter 2 Embryogenesis in Arabidopsis: Signaling, Genes, and the Control of Identity 21 D. L. C. Kumari Fonseka, Xiyan Yang, Anna Mudge, Jennifer F. Topping, and Keith Lindsey Introduction 21 Cellular Events 21 Genes and Signaling – the Global Picture 23 Coordination of Genes and Cellular Processes: a Role for Hormones 25 Genes and Pattern 30 Conclusion and Future Directions 36 References 36 Chapter 3 Endosperm Development 43 Odd-Arne Olsen and Philip W. Becraft Introduction 43 Overview of Endosperm Structure and Development 43 Endosperm Cell Fate Specification and Differentiation 48 Genomic Resources 53 Transcriptional Profiling of Endosperm Development 54 Gene Imprinting in Cereal Endosperm 56 Conclusion 57 Acknowledgments 58 References 58 Chapter 4 Epigenetic Control of Seed Gene Imprinting 63 Christian A. Ibarra, Jennifer M. Frost, Juhyun Shin, Tzung-Fu Hsieh, and Robert L. Fischer Introduction 63 Genomic Imprinting and Parental Conflict Theory 63 Epigenetic Regulators of Arabidopsis Imprinting 65 Mechanisms Establishing Arabidopsis Gene Imprinting 69 Imprinting in the Embryo 74 Imprinting in Monocots 75 Evolution of Plant Imprinting 77 Conclusion 78 Acknowledgments 78 References 78 Chapter 5 Apomixis 83 Anna M. G. Koltunow, Peggy Ozias-Akins, and Imran Siddiqi Introduction 83 Biology of Apomixis in Natural Systems 84 Phylogenetic and Geographical Distribution of Apomixis 89 Inheritance of Apomixis 90 Genetic Diversity in Natural Apomictic Populations 93 Molecular Relationships between Sexual and Apomictic Pathways 94 Features of Chromosomes Carrying Apomixis Loci and Implications for Regulation of Apomixis 95 Genes Associated with Apomixis 96 Transferring Apomixis to Sexual Plants: Clues from Apomicts 97 Synthetic Approach to Building Apomixis 98 Synthetic Clonal Seed Formation 102 Conclusion and Future Prospects 103 References 103 Chapter 6 High-Throughput Genetic Dissection of Seed Dormancy 111 Jose M. Barrero, Colin Cavanagh, and Frank Gubler Introduction 111 Profiling of Transcriptomic Changes 113 Use of New Sequencing Platforms and Associated Techniques to Study Seed Dormancy 114 Visualization Tools 116 Coexpression Studies and Systems Biology Approaches 116 Mapping Populations for Gene Discovery 117 Perspective 118 Acknowledgments 119 References 119 Chapter 7 Genomic Specification of Starch Biosynthesis in Maize Endosperm 123 Tracie A. Hennen-Bierwagen and Alan M. Myers Introduction 123 Overview of Starch Biosynthetic Pathway 124 Genomic Specification of Endosperm Starch Biosynthesis in Maize 126 Conclusion 134 References 134 Chapter 8 Evolution, Structure, and Function of Prolamin Storage Proteins 139 David Holding and Joachim Messing Introduction 139 Prolamin Multigene Families 139 Endosperm Texture and Storage of Prolamins 143 Conclusion 154 References 154 Chapter 9 Improving Grain Quality: Wheat 159 Peter R. Shewry Introduction 159 Grain Structure and Composition 159 End Use Quality 161 Redesigning the Grain 163 Manipulation of Grain Protein Content and Quality 163 Manipulation of Grain Texture 167 Development of Wheat with Resistant Starch 168 Improving Content and Composition of Dietary Fiber 169 Wheat Grain Cell Walls 169 Conclusion 173 Acknowledgments 173 References 173 Chapter 10 Legume Seed Genomics: How to Respond to the Challenges and Potential of a Key Plant Family? 179 Mélanie Noguero, Karine Gallardo, Jérôme Verdier, Christine Le Signor, Judith Burstin, and Richard Thompson Introduction 179 Development of Genomics Tools 180 Applications of Genomics Tools to Legume Seed Biology 185 Future Challenges 192 References 193 Chapter 11 Cotton Fiber Genomics 203 Xueying Guan and Z. Jeffrey Chen Introduction 203 Cotton Fiber Development 204 Roles for Transcription Factors in Development of Arabidopsis Leaf Trichomes, Seed Hairs, and Cotton Fibers 204 Fiber Cell Expansion through Cell Wall Biosynthesis 208 Regulation of Phytohormones during Cotton Fiber Development 209 Cotton Fiber Genes in Diploid and Tetraploid Cotton 210 Roles for Small RNAs in Cotton Fiber Development 211 Conclusion 212 References 213 Chapter 12 Genomic Changes in Response to 110 Cycles of Selection for Seed Protein and Oil Concentration in Maize 217 Christine J. Lucas, Han Zhao, Martha Schneerman, and Stephen P. Moose Introduction 217 Background on the Illinois Long-Term Selection Experiment 217 Phenotypic Responses to Selection 219 Additional Traits Affected by Selection 220 Unlimited Genetic Variation? 221 Genetic Response to Selection: QTL Mapping in the Crosses of IHP x ILP and IHO x ILO 222 New Mapping Population: Illinois Protein Strain Recombinant Inbreds 223 Characterization of Zein Genes and Their Expression in Illinois Protein Strains 225 Contribution of Zein Regulatory Factor Opaque2 to Observed Responses to Selection in Illinois Protein Strains 227 Major Effect QTL May Explain IRHP Phenotype 228 Zein Promoter-Reporter Lines to Investigate Regulation of 22-kDa α-Zein Gene Expression in Illinois Protein Strains 229 Regulatory Changes in FL2-mRFP Expression When Crossed to Illinois Protein Strains 230 Regulation of FL2-mRFP 232 Acknowledgments 233 References 234 Chapter 13 Machine Vision for Seed Phenomics 237 Jeffery L. Gustin and A. Mark Settles Introduction 237 High-Energy Imaging: X-ray Tomography and Fluorescence 238 Optical Imaging: Visible Spectrum 240 Resonance Absorption: Infrared Spectrum 242 Resonance Emission: Nuclear Magnetic Resonance 245 Conclusion 246 Acknowledgments 246 References 246 Color plate section found between pages 42 and 43. Index 253
£166.46
John Wiley and Sons Ltd Root Genomics and Soil Interactions
Book SynopsisFully integrated and comprehensive in its coverage, Root Genomics and Soil Interactions examines the use of genome-based technologies to understand root development and adaptability to biotic and abiotic stresses and changes in the soil environment.Table of ContentsContributors ix Preface xv Chapter 1 Genomics of Root Development 3Boris Parizot and Tom Beeckman Introduction 3 Genomics of LRI 7 Rise of New Technologies to Understand Lateral Root Development 19 ComparativOmics, the Future 20 Acknowledgments 21 References 21 Chapter 2 The Complex Eukaryotic Transcriptome: Nonprotein-Coding RNAs and Root Development 29F. Ariel, A.B. Moreno, F. Bardou, and M. Crespi Genomic Approaches Reveal Novel Aspects of the Eukaryotic Transcriptome 29 The Role of RNA-Binding Proteins in npcRNA Metabolism and Activity 34 Nonprotein-Coding RNAs in Root Development 38 Future Perspectives 42 Acknowledgments 42 References 42 Chapter 3 Genomics of Auxin Action in Roots 49Elisabeth L. Williams and Ive De Smet Introduction 49 The Basis of Auxin Biology 49 Auxin Genomics in Root Development 55 Auxin and Root Hair Development 56 Auxin in Gravitropism 57 Auxin in LR Initiation 57 Conclusion 58 Acknowledgments 58 References 58 Chapter 4 Cell-Type Resolution Analysis of Root Development and Environmental Responses 63Jose R. Dinneny Introduction 63 Tools for Cell-Type Resolution Analysis 64 Analysis of Spatiotemporal Expression Patterns in the Arabidopsis Root 69 Analysis of Cell-Type-Specific Expression Patterns in the Rice Root 70 Cell-Type-Specific Analysis of Auxin 71 Cell-Type-Specific Analyses of Chromatin 71 Cell-Type-Specific Analyses of Responses to Environmental Change 72 Future Prospects 76 Acknowledgments 76 References 77 Chapter 5 Toward a Virtual Root: Interaction of Genomics and Modeling to Develop Predictive Biology Approaches 79Julien Lavenus, Leah Band, Alistair Middleton, Michael Wilson, Mikael Lucas, Laurent Laplaze, and Malcolm Bennett Assembling Root Gene Regulatory Pathways Using Genomics 79 Modeling Well-Characterized Small Root Gene Regulatory Networks 81 Building New Large-Scale Root Gene Regulatory Network 84 Multi-Scale Modeling Approaches to Study Root Growth and Development 88 Conclusions and Future Challenges 89 References 91 Chapter 6 Genomics of Root Hairs 93Hyung-Taeg Cho Genomics with Single Cells 93 Root Hair Development 94 High-Throughput Approaches for the Characterization of Root Hairs 95 Functions of Root Hair-Specific Genes 103 The Regulatory Pathway for Root Hair-Specific Genes 110 Perspective 111 Acknowledgments 111 References 112 Chapter 7 The Effects of Moisture Extremes on Plant Roots and Their Connections with Other Abiotic Stresses 117Laura M. Vaughn and Henry T. Nguyen Introduction 117 Low Water Availability—Drought 118 Excess Water—Soil Waterlogging, Flooding, and Submergence 128 Common Plant Root Responses to Abiotic Stressors 135 Continuing Challenges in Breeding for Plant Root Systems Tolerant to Abiotic Stress 137 Acknowledgments 138 References 138 Chapter 8 Legume Roots and Nitrogen-Fixing Symbiotic Interactions 145Philippe Laporte, Andreas Niebel, and Florian Frugier Genetic Dissection of the Legume Root System 145 Functional Genomic Analyses of Legume Nodules and Roots 155 Concluding Remarks 161 Acknowledgments 162 References 162 Chapter 9 What the Genomics of Arbuscular Mycorrhizal Symbiosis Teaches Us about Root Development 171Damien Formey, Cyril Jourda, Christophe Roux, and Pierre-Marc Delaux Forward and Reverse Genetics for Identifying Myc Mutants 172 Comparative Transcriptomics of AM Symbiosis: Toward Identification of Genes Involved in Root Development 175 Small RNAs in AM Symbiosis 181 Acknowledgments 183 References 183 Chapter 10 How Pathogens Affect Root Structure 189Michael Quentin, Tarek Hewezi, Isabelle Damiani, Pierre Abad, Thomas Baum, and Bruno Favery Introduction 189 Root Infection and Feeding Cell Ontogenesis 190 Genome-Wide Analysis of the Plant Response to Infection 192 The Plant Cytoskeleton Is Targeted by Root Pathogens 193 Root Pathogens Hijack Cell Cycle Regulators 194 Severe Cell Wall Remodeling Is Associated with Feeding Site Formation 195 Phytohormones Regulating Development and Defense May Control Feeding Site Formation 196 Role of miRNAs in Feeding Site Formation and Function 198 Nematode Effectors That Alter Root Cell Development during Parasitism 200 Conclusion 203 Acknowledgments 204 References 204 Chapter 11 Genomics of the Root—Actinorhizal Symbiosis 211Valerie Hocher, Nicole Alloisio, Laurent Laplaze, Didier Bogusz, and Philippe Normand Introduction 211 Actinorhizal Symbiosis 212 Development of Actinorhizal Nodules 214 Genomic Resources for Studying Actinorhizal Symbiosis 217 What Did We Learn from Actinorhizal Genomics? 220 Conclusion and Future Directions 222 Acknowledgments 222 References 223 Chapter 12 Plant Growth Promoting Rhizobacteria and Root Architecture 227Thais L.G. Carvalho, Paulo C.G. Ferreira, and Adriana S. Hemerly Introduction 227 Different Root Niches for PGPR Colonization 228 PGPR Recognition by Plants 229 Modulation of Root Growth and Architecture by PGPRs 232 Mechanisms of Plant Growth Promotion by PGPRs 234 Plant Genetic Programs Controlling Modulation of Root Growth and Architecture by PGPRs 240 Conclusions 241 Acknowledgments 242 References 242 Chapter 13 Translational Root Genomics for Crop Improvement 249Reyazul Rouf Mir, Mahendar Thudi, Siva K. Chamarthi, L. Krishnamurthy, Pooran M. Gaur, and Rajeev K. Varshney Introduction 249 Molecular Dissection of Root Trait 258 Molecular Breeding for Root Traits 259 Summary and Outlook 260 Acknowledgments 260 References 260 Index 265
£171.86
John Wiley & Sons Inc Infrared Spectroscopy of Biomolecules
Book SynopsisThis edited volume spans the latest developments and applications in a new and exciting field of spectroscopy - Fourier Transform Infra-Red (FTIR) spectroscopy.Table of ContentsTheoretical Analyses of the Amide I Infrared Bands of GlobularProteins (H. Torii & M. Tasumi). Fourier Transform Infrared Spectroscopy of Enzyme Systems (J.Alben). Light-Induced Fourier Transform Infrared Difference Spectroscopy ofthe Primary Electron Donor in Photosynthetic Reaction Centers (E.Nabedryk). Equipment: Slow and Fast Infrared Kinetic Studies (F.Siebert). Ultrafast Infrared Spectroscopy of Biomolecules (B. Cohen & R.Hochstrasser). Infrared Spectroscopy of Nucleic Acids (J. Liquier & E.Taillandier). Fourier Transform Infrared Spectroscopy in the Study of HydratedLipids and Lipid Bilayer Membranes (R. Lewis & R.McElhaney). Fourier Transform Infrared Spectroscopic Studies of Cell SurfacePolysaccharides (K. Brandenburg & U. Seydel). Fourier Transform Infrared Spectroscopy of Biomembrane Systems (P.Haris & D. Chapman). What Can Infrared Spectroscopy Tell Us About the Structure andComposition of Intact Bacterial Cells? (D. Naumann, et al.). Biomedical Infrared Spectroscopy (M. Jackson & H.Mantsch). New Trends in Isotope-Edited Infrared Spectroscopy (H. Fabian, etal.). Index.
£223.16
John Wiley & Sons Inc Encyclopedia of Molecular Biology 4 Volume Set
Book SynopsisThe field of molecular biology has revolutionized the study of biology. The applications to medicine are enormous, ranging from diagnostic techniques for disease and genetic disorders, to drugs, to gene therapy. Focusing on the fundamentals of molecular biology and encompassing all aspects of the expression of genetic information, the Encyclopedia of Molecular Biology will become the first point of reference for both newcomers and established professionals in molecular biology needing to learn about any particular aspect of the field.
£2,168.05
John Wiley & Sons Inc InSitu PCR Techniques
Book SynopsisThis book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.Table of ContentsOverview; Review of the PCR Technique; Preliminary Solution-Based Reactions; Preparation of Glass Slides and Tissues; In Situ PCR: DNA and RNA Targets; Special Applications of In Situ Amplification; Hybridization Reactions; Validation and Controls; Materials and Methods; Select Biography; Appendices; Index; About the Authors.
£125.96
John Wiley & Sons Inc Applied Antisense Oligonucleotide Technology
Book SynopsisThis text aims to address the need for investigators to understand the basic concepts, as well as the practical concerns, associated with the use of antisense oligonucleotides in modifying gene expression.Trade Review"This book will be most useful to workers in the field and advanced graduate students." --The Quarterly Review of Biology, June 1999Table of ContentsCHEMISTRY, OLD AND NEW. Oligonucleoside Methylphosphonates: Synthesis and Properties (P. Miller). Oligo(Nucleoside Phosphorothioate)s (P. Guga, et al.). Modified Oligodeoxynucleotides as Antisense Therapeutics (P. Seeberger & M. Caruthers). Novel Chemistry (K. Altmann, et al.). OLIGONUCLEOTIDE INTERNALIZATION, MECHANISM OF ACTION, AND NON-SEQUENCE SPECIFICITY. Cellular Uptake and Biodistribution of Oligodeoxynucleotides (B. Hanss, et al.). Use of Cationic Lipid Complexes for Antisense Oligonucleotide Delivery (C. Bennett). Nonantisense Effects of Antisense Oligonucleotides (L. Neckers & K. Iyer). Ribonuclease H-Mediated Antisense Effects of Oligonucleotides and Controls for Antisense Experiments (D. Tidd). SEQUENCE-SPECIFIC INHIBITION OF GENE EXPRESSION. The Development of C-5 Propyne Oligonucleotides as Inhibitors of Gene Function (W. Flanagan & R. Wagner). The Use of Antisense Oligonucleotides to Inhibit Expression of Isozymes of Protein Kinase C (N. Dean, et al.). BCR-ABL as a Target for Antisense Intervention (S. O'Brien & T. Smetsers). The NF-kB Transcription Factor (R. Narayanan). Disruption of the Map Kinase Signaling Pathway Using Antisense Oligonucleotide Inhibitors Targeted to RAS and RAF Kinase (B. Monia). Protein Kinase A-Directed Antisense Blockade of Cancer Growth: Single Gene-Based Therapeutic Approach (Y. Cho-Chung). Use of Antisense Oligonucleotides in the Central Nervous System: Why Such Success? (M. McCarthy). APPLIED ANTISENSE OLIGONUCLEOTIDE THERAPEUTICS. Perturbing Hematopoietic Cell Gene Expression with Oligodeoxynucleotides: Research and Clinical Applications (A. Gewirtz & M. Ratajczak). BCL2 (B. Jansen & B. Brown). Biological Activity of Guanosine Quartet-Forming Oligonucleotides (R. Rando & M. Hogan). OLIGONUCLEOTIDES AS ANTI-HIV AGENTS. Perspectives on Antisense Technology Against HIV (J. Gee, et al.). In Vivo Pharmacokinetics of Oligonucleotides (S. Agrawal). Early Clinical Trails with Gem 91, A Systemic Oligodeoxynucleotide (R. Martin). THERAPEUTIC OLIGONUCLEOTIDE DATA BASE: PHARMACOKINETICS, IMMUNE STIMULATION, AND USE AS ANTIRESTENOTIC AGENTS. Antisense Therapy to Inhibit Angioplasty Restenosis (L. Rabbani & W. Wang). Pharmacokinetics of Oligonucleotides: A Review of Current Knowledge and Issues for the Future (T. Wallace & P. Cossum). Leukocyte Stimulation by Oligodeoxynucleotides (A. Krieg). SELECTED OLIGODEOXYNUCLEOTIDE DEVELOPMENTAL TOPICS: SPLICING AND TRIPLEXES. Modification of Alternative Splicing of Pre-mRNA by Antisense Oligonucleotides (R. Kole). Gene-Targeted Triple-Helix-Forming Oligonucleotides (F. Svinarchuk & C. Malvy). Triplex-Forming Oligonucleotides for Genetic Manipulation: An Alternative View (A. Faruqi & P. Glazer). A REVIEW OF RIBOZYME TECHNOLOGY. Therapeutic Ribozymes: Principles, Applications, and Problems (J. Rossi). Index.
£250.16
John Wiley & Sons Inc Plant Breeding Reviews Volume 15
Book SynopsisPlant Breeding Reviews is an ongoing series presenting state-of-the art review articles on research in plant genetics, especially the breeding of commercially important crops. Articles perform the valuable function of collecting, comparing, and contrasting the primary journal literature in order to form an overview of the topic. This detailed analysis bridges the gap between the specialized researcher and the broader community of plant scientists.Table of ContentsDedication: Arnel R. Hallauer, Scientist, Maize Breeder,Quantitative Geneticist (K. Lamkey). Molecules Involved in Self-Incompatibility in Flowering Plants (P.Dodds, et al.). Genetic Mosaics and Plant Improvement (M. Marcotrigiano & T.Gradziel). Quantitative Trait Loci: Separating, Pyramiding, and Cloning (Y.Xu). Doubled Haploid Breeding in Cereals (S. Raina). Spelt: Agronomy, Genetics, and Breeding (K. Campbell). Cowpea Breeding (A. Hall, et al.). Recurrent Selection in Soybean (K. Lewers & R. Palmer). Gene Action and Plant Breeding (D. Fasoula & V. Fasoula). Indexes.
£278.96
John Wiley & Sons Inc Monte Carlo Methods in Chemical Physics Volume
Book SynopsisIn Monte Carlo Methods in Chemical Physics: An Introduction to the Monte Carlo Method for Particle Simulations J. Ilja Siepmann Random Number Generators for Parallel Applications Ashok Srinivasan, David M. Ceperley and Michael Mascagni Between Classical and Quantum Monte Carlo Methods: Variational QMC Dario Bressanini and Peter J. Reynolds Monte Carlo Eigenvalue Methods in Quantum Mechanics and Statistical Mechanics M. P. Nightingale and C.J. Umrigar Adaptive Path-Integral Monte Carlo Methods for Accurate Computation of Molecular Thermodynamic Properties Robert Q. Topper Monte Carlo Sampling for Classical Trajectory Simulations Gilles H. Peslherbe Haobin Wang and William L. Hase Monte Carlo Approaches to the Protein Folding Problem Jeffrey Skolnick and Andrzej Kolinski Entropy Sampling Monte Carlo for Polypeptides and Proteins Harold A. Scheraga and Minh-Hong Hao Macrostate Dissection of Thermodynamic Monte Carlo Integrals Bruce W. Church, Alex Ulitsky, and David Shalloway Simulated AnTable of ContentsAn Introduction to the Monte Carlo Method for Particle Simulations (J. Siepmann). Random Number Generators for Parallel Applications (A. Srinivasan, et al.). Between Classical and Quantum Monte Carlo Methods: "Variational" QMC (D. Bressanini & P. Reynolds). Monte Carlo Eigenvalue Methods in Quantum Mechanics and Statistical Methods (M. Nightingale & C. Umrigar). Adaptive Path-Integral Monte Carlo Methods for Accurate Computation of Molecular Thermodynamic Properties (R. Topper). Monte Carlo Sampling for Classical Trajectory Simulations (G. Peslherbe, et al.). Monte Carlo Approaches to the Protein Folding Problem (J. Skolnick & A. Kolinski). Entropy Sampling Monte Carlo for Polypeptides and Proteins (H. Scheraga & M. Hao). Macrostate Dissection of Thermodynamic Monte Carlo Integrals (B. Church, et al.). Simulated Annealing-Optimal Histogram Methods (D. Ferguson & D. Garrett). Monte Carlo Methods for Polymeric Systems (J. de Pablo & F. Escobedo). Thermodynamic-Scaling Methods in Monte Carlo and Their Application to Phase Equilibria (J. Valleau). Semigrand Canonical Monte Carlo Simulation: Integration Along Coexistence Lines (D. Kofke). Monte Carlo Methods for Simulating Phase Equilibria of Complex Fluids (J. Siepmann). Reactive Canonical Monte Carlo (J. Johnson). New Monte Carlo Algorithms for Classical Spin Systems (G. Barkema & M. Newman). Indexes.
£405.86
John Wiley & Sons Inc Tandem Mass Spectrometry 2 Wiley Series on Mass
Book SynopsisThis volume describes the complete process of analyzing protein sequences by mass spectrometry including sample preparation, analysis, and data interpretation at a practical level. It provides sufficient experimental detail to carry out the experiments and consistently achieve good results.Trade Review"[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." (SciTech Book News Vol. 25, No. 2 June 2001) "...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)Table of ContentsAn Introduction to Protein Sequencing Using Tandem Mass Spectrometry. The Primary Structure of Proteins and a Historical Overview of Protein Sequencing. Fundamental Mass Spectrometry. Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra. Basic Polyacrylamide Gel Electrophoresis. The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments. Mass Spectrometric Analysis of Tryptic Digests. Protein Identification by Database Searching. Sequence Analysis of Novel Proteins. The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry. Index.
£147.56
John Wiley & Sons Inc Genomics
Book SynopsisA unique exploration of the principles and methods underlying the Human Genome Project and modern molecular genetics and biotechnology-from two top researchers In Genomics, Charles R. Cantor, former director of the Human Genome Project, and Cassandra L. Smith give the first integral overview of the strategies and technologies behind the Human Genome Project and the field of molecular genetics and biotechnology. Written with a range of readers in mind-from chemists and biologists to computer scientists and engineers-the book begins with a review of the basic properties of DNA and the chromosomes that package it in cells. The authors describe the three main techniques used in DNA analysis-hybridization, polymerase chain reaction, and electrophoresis-and present a complete exploration of DNA mapping in its many different forms. By explaining both the theoretical principles and practical foundations of modern molecular genetics to a wide audience, the book brings the scientific communiTrade Review"this book is to be highly recommended..."(Human Genetics, May 2000)Table of ContentsDNA Chemistry and Biology. A Genome Overview at the Level of Chromosomes. Analysis of DNA Sequences by Hybridization. Polymerase Chain Reaction and Other Methods for In Vitro DNA Amplification. Principles of DNA Electrophoresis. Genetic Analysis. Cytogenetics and Pseudogenetics. Physical Mapping. Enhanced Methods for Physical Mapping. DNA Sequencing: Current Tactics. Strategies for Large-Scale DNA Sequencing. Future DNA Sequencing Without Length Fractionation. Finding Genes and Mutations. Sequence-Specific Manipulation of DNA. Results and Implications of Large-Scale DNA Sequencing. Appendix. Index.
£183.56
John Wiley & Sons Inc Opportunities Nutrigenomics The Opportunities and
Book SynopsisAs with all biotechnologies in the midst of rapid growth, nutrigenomics faces ethical, legal, and social implications that are likely to affect the public and the medical community. This title identifies and examines the anticipated risks and expected benefits of nutrigenomics from an ethical perspective.Trade Review"…a worthy acquisition for any medical library…along with students and instructors in nutrition and health care." (CHOICE, June 2007) "This concise overview of nutritional genomics covers a variety of topics surrounding this controversial topic." (Doody's Health Services)Table of ContentsPreface. Acknowledgments. 1 NUTRITIONAL GENOMICS: OPPORTUNITIES AND CHALLENGES. 1.1 Introduction. 1.2 What is Nutritional Genomics? 1.3 Methodology and Approach of this Book. 1.4 Opportunities and Challenges for Nutrigenomics. 1.4.1 Improved health. 1.4.2 Personalized dietary advice. 1.4.3 Improved diet. 1.4.4 More development of health-enhancing food products. 1.4.5 Consumer empowerment. 1.4.6 Reducing health disparities. 1.4.7 Health care savings. 1.5 Challenges and a Road Map of This Book. References. 2 THE SCIENCE OF NUTRIGENOMICS AND NUTRIGENETICS. 2.1 Introduction. 2.2 The Scientific Context. 2.2.1 Nutrigenomics. 2.2.2 Nutrigenetics. 2.3 The Case of MTHFR. 2.4 Room for Improvement. 2.4.1 Study design. 2.4.2 Epigenetics. 2.4.3 SNPs and haplotypes. 2.4.4 Dietary intake assessment. 2.4.5 Biomarkers. 2.4.6 Susceptibility and predictions. 2.4.7 Analytical and clinical validity. 2.4.8 Clinical utility. 2.5 Science and Technology Assessment. 2.6 Conclusion. References. 3 THE ETHICS OF NUTRIGENOMIC TESTS AND INFORMATION. 3.1 Introduction. 3.2 Ethical Principles. 3.3 Nutrigenomics Testing in the Clinical Setting. 3.3.1 Informed consent. 3.3.2 Confidentiality. 3.3.3 Secondary information. 3.3.4 Families. 3.3.5 Genetic testing of children and adolescents. 3.4 Use of Nutrigenomics Information for Research. 3.5 Use of Nutrigenomics Information by Private Third Parties. 3.5.1 Insurance. 3.5.2 Employment. 3.5.3 Legal and social responses to fears of discrimination. 3.6 Conclusion. References. 4 ALTERNATIVES FOR NUTRIGENOMIC SERVICE DELIVERY. 4.1 Introduction. 4.2 Considerations for Nutrigenomic Service Delivery. 4.2.1 Strength of the science. 4.2.2 Regulatory environment. 4.2.3 Human resource capacity and professional competence. 4.2.4 Funding policy. 4.2.5 Professional politics and culture. 4.2.6 Consumers and patients. 4.3 Four Alternative Models. 4.3.1 Consumer model. 4.3.2 Health practitioner model. 4.3.3 Blended models. 4.3.4 Public health model. 4.4 Conclusion. References. 5 NUTRIGENOMICS AND THE REGULATION OF HEALTH CLAIMS FOR FOODS AND DRUGS. 5.1 Introduction. 5.1.1 Genetic tests, service delivery, and genetic antidiscrimination. 5.2 Food Categories: Functional Foods, Nutraceuticals, Medicinal Foods, and Dietary Supplements. 5.2.1 Functional foods. 5.2.2 Nutraceuticals. 5.2.3 Medical or medicinal foods. 5.2.4 Dietary supplements. 5.3 Health-Related Claims Associated with Foods Compared to Drugs. 5.3.1 Structure–function claims. 5.3.2 Health claims. 5.3.3 Medical food claims. 5.3.4 Disease risk reduction claims. 5.4 Nutrigenomic Information and the Regulation of Foods Compared to Drugs. 5.4.1 The regulation of foods. 5.4.2 The regulation of drugs. 5.5 Food and Drug Regulations in Japan, the United States, and Canada. 5.5.1 Japan. 5.5.2 United States. 5.5.3 Canada. 5.6 Conclusion. References. 6 NUTRIGENOMICS: JUSTICE, EQUITY, AND ACCESS. 6.1 Introduction. 6.2 Industrialized Country Context. 6.2.1 Individualized nutrigenomic testing. 6.2.2 Population-based nutrigenomics. 6.3 Developing Country Context. 6.3.1 Individualized nutrigenomic testing. 6.4 Nutrigenomics and Intellectual Property. 6.4.1 An issue of access to scientific information. 6.5 Conclusion. References. 7 CONCLUSIONS AND RECOMMENDATIONS. 7.1 Introduction. 7.1.1 Nutrigenomic science. 7.1.2 Nutrigenomics and health information management. 7.1.3 Nutrigenomic service delivery. 7.1.4 Regulation of nutrigenomics. 7.1.5 Access and equity. 7.2 A Final Word. Index.
£75.56
John Wiley & Sons Inc Genetic Techniques for Biological Research A Case
Book SynopsisThis advanced textbook teaches the theory and practice of molecular genetic analysis to senior undergraduates and graduates studying genetics, molecular biology and cell biology. It uses a case study approach, with the yeast Saccharomyces as the model genetic organism, to explain the theory and practice of molecular genetic analysis.Trade Review"...Students new to yeast genetics and those of us keeping abreast of fusing disciplines should add this publication to our reading list..." (The Biochemist, 4 October 2002) "... useful addition to the literature..." (Biologist, Vol.50. No.2, 2003) "...a valuable addition to the reference library of any research laboratory at universities, research institutes and the industry." (Acta Biotechnologica, Vol.23, No.2-3, 2003) "...The aim of every teacher is to inspire students to move down from the stands, step up to the plate, and start hitting on their own. This book is a wonderful tool to help in that quest." (Cell Biology Education, 16 June 2003)Table of ContentsIntroduction. SECTION I: SACCHAROMYCES CEREVISIAE AS A GENETIC RESEARCH ORGANISM. Saccharomyces cerevisiae As a Genetic Model Organism Techniques in Cell and Molecular Biology Saccharomyces Cell Structure. SECTION II: TECHNIQUES OF GENETIC ANALYSIS. Mutant Hunts - To Select or to Screen (perhaps even by brute force). Complementation Analysis: How many Genes are Involved? Epistasis Analysis. Gene Isolation and Analysis of Multiple Mutant Alleles. Suppression Analysis. Enhancement and Synthetic Phenotypes Two-hybrid Analysis Advanced Concepts in Molecular Genetic Analysis Genomic Analysis SECTION III: CASE STUDIES FROM THE SACCHAROMYCES GENETIC LITERATURE. Case Study I: Glucose Sensing and signaling Mechanisms in Saccharomyces. Case Study II: Secretion, Exocytosis and Vesicle Trafficking in Saccharomyces. Case Study III: The Cell Division Cycle of Saccharomyces. Case Study IV: Mating-type Pheromone Response Pathway of Saccharomyces. Index.
£239.36
John Wiley & Sons Inc Gene Therapy Technologies Applications and
Book SynopsisGene Therapy Technologies, Applications and Regulations From Laboratory to Clinic Edited by Anthony Meager Division of Immunobiology, The National Institute for Biological Standards and Control, South Mimms, UK The development of gene-based technologies has been rapid over the past decade and has consequently resulted in a surge of interest in human gene therapy, the deliberate transfer of genes to somatic cells to cure or alleviate disease symptoms. Hundreds of clinical protocols involving variously designed vectors for efficient gene transfer have been developed. However, the use of such complex ''gene medicines'' containing potentially heritable genes has raised numerous concerns regarding quality, efficacy and safety. Encompassing recent developments in the field and addressing current concerns this book: * surveys many of the current technologies for preparing vectors for use in gene therapy protocols * reviews the application of gene-mediated therapies to a range of medicTrade Review"It succeeds in putting together, possibly for the first time, gene therapy vectors, clinical applications, and regulatory production issues." --Human Genetics, April 2000Table of ContentsCationic Liposomes for Gene Therapy Applications (J. Clancy & E. Sorscher). DNA Condensation and Receptor-mediated Gene Transfer (A. Ziady & T. Ferkol). Retroviral Vectors (W. Günzburg & B. Salmons). Lentiviral Vectors (A. Lever). Adenoviral Vectors (S. Connelly). Adeno-associated Viral Vectors (T. Flotte & B. Carter). Advances in Engineering HSV Vectors for Gene Transfer to the Nervous System (M. Soares, et al.). Mammalian Artificial Chromosomes: Prospects for Gene Therapy (T. Ebersole & C. Farr). Gene Therapy for Severe Combined Immunodeficiency (A. Thrasher, et al.). Gene Therapy for Haemophilia (R. Hoeben, et al.). Cystic Fibrosis: Gene Therapy Approaches (N. Caplen). Therapeutic Approaches to Haemoglobinopathies (Y. Beuzard). Gene Therapy Approaches to Duchenne Muscular Dystrophy (S. Murphy & G. Dickson). Lysosomal Storage Diseases (L. Lashford, et al.). Prospects for Gene Therapy of HIV Infections and AIDS (C. Lee, et al.). The Developemnt of the Regulatory Process in Europe for Biological Medicines: How it Affects Gene Therapy Products (A. Meager, et al.). Developement and Regulation of Gene Therapy Drugs in Germany (K. Cichutek). The Transfer of Technology from the Laboratory to the Clinic: In Process Controls and Final Product Testing (F. Borellini & J. Ostrove). Good Laboratory Practice in the Research and Development Laboratory (A. Shepherd). Facilities for Large-scale Production of Vectors Under GMP Conditions (J. Boyd). Addendum. Index.
£250.16
John Wiley & Sons Inc Coumarins
Book SynopsisCoumarins: Biology, Applications and Mode of Action predominantly focuses on the parent compound, coumarin, and its main metabolite in humans, 7-hydroxycoumarin. It describes in detail every facet of these compounds including history, toxicology, chemistry, metabolism, analysis, clinical, veterinary and other applications, their roles as immunomodulatory agents and speculates on their mode of action.Table of ContentsPartial table of contents: History of the Development and Applications of Coumarin and Coumarin-related Compounds (I. Weinmann). The Chemistry and Occurrence of Coumarins (G. Keating & R. O'Kennedy). Coumarin as an Immunomodulator (G. Zlabinger). Mode of Action of Coumarin in the Treatment of Thermal Injuries (N. Piller). The Potential Role of Coumarins in the Therapy of Prostate Cancer (J. Mohler, et al.). Clinical and Biological Observations Associated with Coumarins (R. Thornes). Analysis of Coumarins (D. Bogan, et al.). Suggested Modes of Action of Coumarins and Some Comments on their Significance (R. O'Kennedy & R. Thornes). Index.
£207.86
John Wiley & Sons Inc Population Genetics of Multiple Loci
Book SynopsisPopulation Genetics of Multiple Loci F.B. Christiansen University of Aarhus, Denmark "This is a very beautiful and powerful study of an area that Christiansen has dominated for many years. " - Marcus Feldman, Stanford University, USA Population genetics thrives on the constant interaction between theoretical and empirical knowledge.Trade Review"...a significant contribution to mathematical population genetics...recommended" --Monatshefte fur Mathematik, Vol 131/2, 2000 "This book makes an excellent contribution." --Genetical Research, Vol 75, 2000Table of ContentsInteractions among Genes. RECOMBINATION AND SEGREGATION. Random Mating: Sexes Equal. Random Mating: Sex Difference. Inbreeding: Partial Selfing. Migration and Mixing. Phenotypic Variation. SELECTION. Viability Selection. Symmetric Viability Selection. Fertility Selection. Mutation and Selection. Migration and Selection. Evolution of Recombination. Glossary. References. Index.
£231.26
University of California Press Engineering Trouble Biotechnology and Its Discontents
Book SynopsisTalk of genetically engineered organisms (GEOs) has moved from the hushed corridors of life science corporations to the front pages of major newspapers. This book examines these issues from the diverse perspectives of sociology, geography, law, environmental studies and political science.Table of ContentsList of Illustrations Acknowledgments Introduction. Biotechnology in the New Millennium: Technological Change, Institutional Change, and Political Struggle Rachel A. Schurman 1. Wonderful Potencies? Deep Structure and the Problem of Monopoly in Agricultural Biotechnology William Boyd 2. Building a Better Tree: Genetic Engineering and Fiber Farming in Oregon and Washington W. Scott Prudham 3. The Migration of Salmon from Nature to Biotechnology Dennis Doyle Takahashi Kelso 4. Making Biotech History: Social Resistance to Agricultural Biotechnology and the Future of the Biotechnology Industry Rachel A. Schurman and William A. Munro 5. Eating Risk: The Politics of Labeling Genetically Engineered Foods Julie Guthman 6. The Global Politics of GEOs: The Achilles' Heel of the Globalization Regime? Frederick H. Buttel 7. Biotech Battles: Plants, Power, and Intellectual Property in the New Global Governance Regimes Kathleen McAfee 8. From Molecules to Medicines: The Use of Genetic Resources in Pharmaceutical Research Astrid J. Scholz 9. The Brave New Worlds of Agricultural Technoscience: Changing Perspectives, Recurrent Themes, and New Research Directions in Agro-Food Studies David Goodman Conclusion. Recreating Democracy Dennis Doyle Takahashi Kelso Glossary Contributors Bibliography Index
£27.00
