Genetics (non-medical) Books

Book SynopsisThis textbook provides step-by-step protocols and detailed explanations for RNA Sequencing, ChIP-Sequencing and Epigenetic Sequencing applications. The reader learns how to perform Next Generation Sequencing data analysis, how to interpret and visualize the data, and acquires knowledge on the statistical background of the used software tools. Written for biomedical scientists and medical students, this textbook enables the end user to perform and comprehend various Next Generation Sequencing applications and their analytics without prior understanding in bioinformatics or computer sciences.Table of Contents

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Book SynopsisThis open access textbook leads the reader from basic concepts of chromatin structure and function and RNA mechanisms to the understanding of epigenetics, imprinting, regeneration and reprogramming. The textbook treats epigenetic phenomena in animals, as well as plants. Written by four internationally known experts and senior lecturers in this field, it provides a valuable tool for Master- and PhD- students who need to comprehend the principles of epigenetics, or wish to gain a deeper knowledge in this field. After reading this book, the student will: Have an understanding of the basic toolbox of epigenetic regulation Know how genetic and epigenetic information layers are interconnected Be able to explain complex epigenetic phenomena by understanding the structures and principles of the underlying molecular mechanisms Understand how misregulated epigenetic mechanisms can lead to disease Table of Contents

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Book SynopsisThis comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.Table of Contents1. The recombinant DNA technology era 1.1 Important timelines 1.2 Scientist and their contribution to recombinant DNA technology 1.3 Recent advances2. Vectors: Guide to gene delivery vehicles 2.1 Plasmid, Cosmid, Phasmid, Bacteriophage 2.2 YAC and BAC 2.3 Shuttle vector 2.4 Expression vector3. Gene isolation methods: Beginners guide 3.1 Isolation of genomic and PCR amplification 3.2 Gene synthesis 3.3 cDNA library 3.4 Genomic library4. Gene cloning strategies: overview 4.1 Restriction and ligation-based cloning 4.2 Ligation-independent cloning 4.3 PCR-based cloning4.4 Recombination-based cloning 4.5 Mating-Assisted Genetically Integrated Cloning5. DNA cutters and connecters in recombinant DNA technology 5.1 RM system 5.2 Restriction enzymes 5.3 Sequence specific, G+C content, Methylation 5.4 Elimination of RM in host6 Fundamental techniques of recombinant DNA transfer 6.1 Chemical transformation in Bacteria, Fungi and animal cell 6.2 Physical transformation in Bacteria, Fungi and animal cell 7. Screening of recombinants 7.1 Colony hybridisation 7.2 Antibiotic based screening 7.3 Colony PCR 7.4 Plasmid Miniprep 7.5 Southern blotting 7.6 DNA Sequencing 7.7 Protein expression SDS PAGE, western blotting8. Recent Trends and Advances 8.1 Basics of gene editing 8.2 Zinc finger nucleases 8.3 Transcription activators like effectors 8.4 RNA interference 8.4 CRISPR Cas system 8.5 Cre-lox mechanism9. Application of Recombinant DNA Technology 9.1 Health, Medical and Diagnostics, Pharmaceuticals, Agriculture, , Industry 9.2 Health: Gene therapy, Diagnostics, 9.3 Pharmaceuticals: Vaccines, Insulin, Antibiotics, antibodies 9.4 Environment: Bioremediation, Bio indicators 9.5 Industry: Enzyme 9.6 Agriculture: Yield improvement, tolerant plants, transgenic for secondary metabolites 10. Ethical and safety concerns of recombinant DNA technology 10.1 Questionable knowledge dissemination 10.2 Uncertainties about misuse of technology 10.3 Potential risk of engineered organisms

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Book SynopsisVarious parallels have been drawn between wolves and humans from the perspective of their social organisation. Therefore, studying wolves may well shed light on the evolutionary origins of complex human cognition and, in particular, on the role that cooperation played in its development. Humans closely share their lives with millions of dogs – the domesticated form of wolves. Biologically, wolves and dogs can be considered to be the same species; yet only dogs are suitable living companions in human homes, highlighting the importance of cognitive and emotional differences between the two forms. The behaviour of wolves and dogs largely depends on the environment the animals grew up and live in. This book reviews more than 50 years of research on the differences and similarities of wolves and dogs. Beyond the socio-ecology, the work explores different theories about when and how the domestication of wolves might have started and which behaviours and cognitive abilities might have changed during this process. Readers will discover how these fascinating animals live with their conspecifics in their social groups, how they approach and solve problems in their daily lives and how they see and interact with their human partners.Trade Review“For the layperson wishing an enticing introduction to the study of wolves and dogs, this book’s several short, highlighted sections will be key. … While this book is readable by an intelligent wolf-dog enthusiast with a dictionary at hand, Wolves and Dogs: between Myth and Science is most approachable by the serious aca­demic. … The book … introduces the reader to basic information about wolves, then takes a deep academic dive into findings and questions around many topics … .” (Nancy jo Tubbs, Nancy joInternational Wolf, Spring, 2023)Table of ContentsSee attachments

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Book SynopsisPreface.- What is a RASopathy.- Neuro?bromatosis Type 1.- Legius Syndrome.- Noonan Syndrome.- Noonan Syndrome with Multiple Lentigines.- Costello Syndrome.- Cardio-facio-cutaneous Syndrome.- Capillary Malformation Arteriovenous Malformation Syndrome.- SYNGAP1 The Gene and Syndrome.- Central Conducting Lymphatic Anomalies.- Defining Pathogenic Variants in RASopathies.- Molecular Genetics of Noonan Syndrome and other RASopathies.- Comprehensive Care for Patients with RASopathies.- Precision Medicine in RASopathies: Tailored Disease Management through Enhanced Approaches, Disease Delineation, and Pathway-Specific Therapies.- Genetic Counseling and the RASopathies.- The Importance of Advocacy in the RASopathies.- The RAS Signaling Network and Cancer.- The RAS-regulated RAF-MEK1/2-ERK1/2 Protein Kinase Pathway the path most travelled in RASopathies.- The non-canonical RAS/MAPK pathway and the RASopathies.- Understanding the Ras in RASopathies.- RAS Family Interactions: The SHOC2-MRAS-PP1 complex in Noonan syndrome.- RASopathy Genes: Germline Risk and Somatic Cancers.- Cancer in Neurofibromatosis type 1.- Prenatal Manifestations of the RASopathies.- Cardiovascular Disease in the RASopathies.- Cognitive Development and Learning in RASopathies.- Behavioral Profile in RASopathies.- Autism and Social Behavior in RASopathies.- Epilepsy in RASopathies.- Speech-Language Pathology in the RASopathies.- Ophthalmic Manifestations in RASopathies.- Dermatological Associations in the RASopathies.- Mosaic RASopathies.- Craniofacial and Dental Development in the RASopathies.- Skeletal Muscle Development in the RASopathies.- Orthopedic Issues of the RASopathies.- Drosophila models of RASopathies.- Neurofibromatosis Type 1 Mouse Models.- Neurofibromatosis type I: Preclinical Modeling.- Modeling the non-NF1 RASopathies.- Clinical Studies and Small Molecule Inhibitors for RASopathy Treatment.- Index.

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Book SynopsisChapter 1 - MDIG in breast cancer progression and metastasis.- Chapter 2 - Tumor Microenvironment and Epigenetic Implications in Breast Cancer Progression.- Chapter 3 - The Epigenetic Landscape of Breast Cancer, Metabolism, and Obesity.- Chapter 4 - Epigenetic Modulations by Microbiome in Breast Cancer.- Chapter 5 - Facilitates chromatin transcription in breast and other cancers.- Chapter 6 - Advances in epigenetic therapeutics for breast cancer.- Chapter 7 - Detection methods for epigenetic mechanisms in breast cancer.

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Book SynopsisChapter 1Introduction.- Chapter 2-Genetics Analysis for Health Analytics.- Chapter 3-Determining Phenotypic Traits from Single Nucleotide Polymorphism (SNP) Data.- Chapter 4-Clinical Genetic Databases: ClinVar, ACMG Clinical Practice Guidelines.- Chapter 5-Inferring Disease Risk from Genetics.- Chapter 6-Challenges in Health Analytics Due to Lack of Diversity in Genetic Research: Implications and Issues with Published Knowledge.- Chapter 7-Clinical Data and Health Data Types.- Chapter 8-Clinical Datasets: Open Access Electronic Health Records Datasets.- Chapter 9-Association Mining with Clinical Data: Phenotype-Wide Association Studies (PheWAS).- Chapter 10-Organizing a Clinical Study Across Multiple Clinical Systems: Common Data Models.- Chapter 11-Environmental Health Data Types for Health Analytics.- Chapter 12-Geospatial Analysis Using Environmental Health Data.- Chapter 13-Social Determinants of Health Data for Health Analytics.- Chapter 14-Geospatial Analysis Using Social Determinants of Health, Clinical Data and Spatial Regression Methods.- Chapter 15Ethics.

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Book SynopsisThe free-radical chemistry of DNA had been discussed in some detail in 1987 in my book The Chemical Basis of Radiation Biology. Obviously, the more recent developments and the concomitant higher level of understanding of mechanistic details are missing. Moreover, in the living cell, free-radical DNA damage is not only induced by ionizing radiation, but free-radical-induced DNA damage is a much more general phenomenon. It was, therefore, felt that it is now timely to review our present knowledge of free-radical-induced DNA damage induced by all conceivable free-radical-generating sources. Originally, it had been thought to include also a very important aspect, the repair of DNA damage by the cell’s various repair enzymes. Kevin Prise (Cancer Campaign, Gray Laboratory, L- don) was so kind to agree to write this part. However, an adequate description of this strongly expanding area would have exceeded the allocated space by much, and this section had to be omitted. The directors of the Max-Planck-Institut für Strahlenchemie (now MPI für Bioanorganische Chemie), Karl Wieghardt and Wolfgang Lubitz, kindly allowed me to continue to use its facilities after my retirement in 2001. Notably, our - brarian, Mrs. Jutta Theurich, and her right-hand help, Mrs. Rosemarie Schr- er, were most helpful in getting hold of the literature. I thank them very much. Without their constant help, this would have been very difficult indeed.Table of ContentsFormation of Reactive Free Radicals in an Aqueous Environment.- The Hydroxyl Radical.- Hydrogen Atom and Hydrated Electron.- Inorganic Radicals.- Carbon-Centered Radicals.- Heteroatom-Centered Radicals.- Peroxyl Radicals.- Polymer Radicals.- Nucleobases, Nucleosides and Nucleotides.- Polynucleotides and Single-Stranded DNA Fragments.- DNA and Double-Stranded Oligonucleotides.- Methods.

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Book SynopsisThis open-access book provides a comprehensive overview of current methodologies for improving resistance to leaf rust in coffee, one of the world's most important cash crops and beverages. Coffea arabica L. (Arabica) accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book introduces essential concepts of mutation breeding as an efficient tool to increase the genetic diversity of Arabica coffee and presents practical methods on mutation induction and screening for resistance to CLR. Current breeding approaches, challenges and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Practical protocols for mutation induction and screening for resistance to CLR are described, including novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter has an introduction and is supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications.Table of ContentsIntroduction coffee breeding and challenges Choice of materials for mutation induction in arabica coffee Improved in-vitro establishment and germination of Coffea arabica seed Induced mutagenesis in coffee (Coffea arabica L.) using chemical agents Mutation induction using gamma irradiation and high frequency embryogenic callus from coffee (Coffea arabica) Chemical mutagenesis of Coffea arabica mature seed using EMS Physical mutagenesis of coffee seeds In-vitro regeneration of Coffea arabica var. Venecia through somatic embryogenesis Protocol on mutation induction in Coffea arabica using in vivo grafting and cuttings Protocol on mutation induction in coffee using in vitro tissue cultures Screening for resistance to coffee leaf rust Protocol to send samples of coffee leaf rust to CIFC Coffee leaf rust (Hemileia vastatrix) inoculation and evaluation under laboratory conditions Development of a PCR-Based Molecular Detection Technique for the Early Diagnosis of Coffee Leaf Rust Caused by Hemileia vastatrix Protocols for chromosome preparations: molecular cytogenetics and studying genome organization in coffee

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Book SynopsisLernen Sie, wie Sie durch die wissenschaftlichen Erkenntnisse der Selbstheilung und der Wechselwirkung zwischen Ernährung und Genom Ihre Gesundheit positiv beeinflussen können. Tauchen Sie ein in die Welt der Epigenetik, die Ihnen erklärt, wie Sie Ihre Gesundheit fördern und das Beste aus sich selbst herausholen können.Zunächst führt Sie das Werk in die molekularen Grundlagen ein, wie jede einzelne Zelle funktioniert und Information über ihre Umgebung verarbeitet. Letzteres hat sehr viel mit Epigenetik zu tun, die ein zentrales Thema in diesem Buch ist. Die Epigenetik, die für die Identität der mehr als 400 verschiedenen Zelltypen unseres Körpers entscheidend ist, hat auch einen dynamischen Aspekt. Sie lernen, wie intrazellulärer Signalübertragungswege über extrazelluläre Signale aktiviert werden und damit Transkriptionsfaktoren und Chromatin-modifizierenden Enzymen beeinflussen. Viele dieser Signale stammen direkt oder indirekt von unserer Ernährung. Letztere ist das wichtigste externe Signal, dem wir täglich ausgesetzt sind. Das führt zum zweiten zentralen Thema dieses Buches, der Nutrigenomik. Die Nahrung, die wir täglich zu uns nehmen, ist weit mehr als eine Ansammlung von Kohlenhydraten, Proteinen, Lipiden, Vitaminen und Mineralien, die Energie und Bausteine für unseren Körper liefert. Das faszinierende Fachgebiet der Nutrigenomik beschreibt die Wechselwirkung zwischen Nahrungsmolekülen, ihren Metaboliten und unserem (Epi)genom. Viele Nahrungsbestandteile, wie beispielsweise ungesättigte Fettsäuren, aktivieren direkt Transkriptionsfaktoren oder verursachen Veränderungen in den Konzentrationen von Metaboliten. Diese beeinflussen wiederum die Aktivität von Chromatin-modifizierenden Enzymen. Auf diese Weise „sprechen“ Moleküle unseres Frühstücks, Mittagessen oder Abendessen mit unserem Genom bzw. Epigenom.Tauchen Sie ein in die faszinierende Welt der Selbstheilung und Verantwortung für Ihre Gesundheit!Table of Contents1 Das menschliche Genom 1.1 Migration und evolutionäre Herausforderungen des Homo sapiens 1.2 Vielfalt menschlicher Populationen 1.3 Varianten des menschlichen Genoms 2 Epigenetik, Chromatin und Genexpression 2.1 Was ist Epigenetik? 2.2 Die epigenetische Landschaft 2.3 Nukleosomen: zentrale Einheiten des Chromatins 2.4 Chromatinarchitektur: Epigenetik in 3D 2.5 Chromatinorganisation 2.6 Epigenetik und Genexpression 3 Epigenetische Regulation 3.1 DNA-Methylierung 3.2 Genetische Prägung 3.3 Histone und ihre Modifikationen: der Histoncode 3.4 Genregulation über Chromatin-modifizierende Enzyme 3.5 Chromatinremodellierer 3.6 Lange ncRNA-Moleküle als Chromatinorganisatoren 4 Molekulare Sensoren von Makro- und Mikronährstoffen 4.1 Grundlagen des Stoffwechsels 4.2 Kohlenhydrate, Aminosäuren und Lipide 4.3 Kernrezeptoren als Nährstoffsensoren 4.4 Vitamine und ihre Rezeptoren 5 Nutrigenomik und Stoffwechselregulation 5.1 Nutrigenomik und Nutrigenetik 5.2 Epigenetische Signalübertragung von Metaboliten des Intermediärstoffwechsel 5.3 Zirkadiane Kontrolle von Stoffwechselprozessen 6 Ernährung und Volkskrankheiten 6.1 Evolution der menschlichen Ernährung 6.2 Ernährung und Stoffwechselerkrankungen 6.3 Ernährung und Krebs 6.4 Molekulare Auswirkungen von körperlicher Aktivität 6.5 Kalorienrestriktion und der zelluläre Energiestatus 7 Embryogenese und Zelldifferenzierung 7.1 Epigenetische Veränderungen während der Embryogenese 7.2 Stammzellen und zelluläre Pluripotenz 7.3 Epigenetische Dynamik während der Differenzierung 8 Das Immunsystem 8.1 Epigenetik der Hämatopoese 8.2 Akute und chronische Entzündungen 8.3 Die Rolle der Epigenetik bei Immunantworten 9 Fettleibigkeit und Diabetes 9.1 Fettleibigkeit 9.2 Entzündung im Fettgewebe 9.3 Metabolischer Stress im ER 9.4 Energiehomöostase und hormonelle Regulation der Nahrungsaufnahme 9.5 Glukosehomöostase und Insulinresistenz 9.6 β-Zell-Versagen und T2D 10 Herz-Kreislauf-Erkrankungen und das metabolische Syndrom 10.1 Bluthochdruck und Arteriosklerose 10.2 Lipoproteine und Dyslipidämien 10.3 Metabolisches Syndrom 11 Epigenetik von Krebs 11.1 Epimutationen bei Krebs 11.2 Epigenom-weite Störungen als Kennzeichen von Krebs 11.3 Epigenetische Umprogrammierung bei Krebs 11.4 Epigenetische Mechanismen von Krebs 12 Das zentrale Nervensystem 12.1 Die Rolle der Epigenetik bei der neuronalen Entwicklung 12.2 Epigenetische Grundlagen des Gedächtnisses 12.3 Epigenetik neurodegenerativer Erkrankungen 13 Ernährung und Altern 13.1 Evolutionäre Anpassungen des menschlichen Genoms 13.2 Generationsübergreifende epigenetische Vererbung 13.3 Konservierte nährstoffassoziierte Signalwege und das Altern 13.4 Epigenetik des Alterns 14 Der gesunde Lebensstil 14.1 Epigenom-weite Varianten 14.2 Personalisierte Medizin und Ernährung 14.3 Epigenetische Therapie von Krankheiten

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Book SynopsisChapter 1. Metagenomics and Its application in environmental monitoring.- Chapter 2. Identification of beneficial microbes from metagenomic sequencing.- Chapter 3. Bioremediation techniques: principles, advantages, limitations and prospects.- Chapter 4. Bacteriophages: Biocontrol Tools in the Era of Antimicrobial Resistance.- Chapter 5. Next-Generation Sequencing: Application and data analysis.- Chapter 6. Genome editing technologies using CRISPR-Cas9.- Chapter 7. Application of nanotechnology in the agriculture and allied sector.- Chapter 8. Recent Developments in Biosensor Technology with Prospective Applications.- Chapter 9. Bioinformatics tools: Insights from structural approaches.- Chapter 10. Microarrays technology: Overview and current Status.- Chapter 11. An overview of quantitative proteomic approaches.- Chapter 12. Mass spectrometry-based approaches in metabolomics.- Chapter 13. A comparative overview of epigenomics.- Chapter 14. Nutrigenomics and its applications.- Chapter 15. Gene cloning and expression analysis.- Chapter 16. Tools for transcriptomics data analysis.

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Book SynopsisChapter 1. Introduction: Breeding Climate Resilient and Future Ready Oilseed Crops.- Chapter 2. Genomics, Pan-Genomics and Super Pan-Genomics of Major Oilseed Crops.- Chapter 3. Genetic Enhancement of Abiotic Stress Tolerance in Oilseeds through Contemporary Breeding Approaches.- Chapter 4. Breeding for Insect Pests Resistance in Oilseed Crops through Genomics Assisted Breeding.- Chapter 5. Breeding oil seed crops for resistance to fungal pathogens through genomics-assisted breeding.- Chapter 6. Genomic Approaches for Achieving Higher Nutrient Use Efficiency in Oilseeds.- Chapter 7. Application of Novel Breeding Methods to Achieve Rapid Genetic Gain in Oilseed Crops.- Chapter 8. Advances in Plant Phenotyping for Climate Resilient Oilseeds Breeding.- Chapter 9. Genome Editing: A Novel Breeding Strategy for Enhanced Stress Tolerance and Quality in Oil Seed Crops.- Chapter 10. Breeding Climate-Resilient Groundnut in the Climate Change Era: Current Breeding Strategies and Prospects.- Chapter 11. Breeding climate resilient Rapeseed-Mustard in climate change era: Current breeding strategies and prospects.- Chapter 12. Breeding Climate Resilient Soybean in Climate Change Era: Current Breeding Strategies and Prospects.- Chapter 13. Breeding climate-resilient sunflowers in the climate change era: Current breeding strategies and prospects.- Chapter 14. Adaption of next-generation breeding tools in the improvement of minor oilseed crops: Status and prospects.- Chapter 15. Breeding for high oleate oilseed crops: Opportunities, constraints, and prospects.- Chapter 16. Global Status of Genetic, Genomic and Bioinformatics Resources for Genetic Enhancement of Oilseed Crops.

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