Biochemistry Books

Book SynopsisThis book provides a comprehensive overview of carotenoid biosynthesis by different organisms, including bacteria, archaea, fungi, arthropods, and plants. Carotenoids are thought to provide health benefits in areas such as cancer, diabetes, osteoporosis, NAFLD, NASH, obesity, age-related functional decline, and as a result, they have received an increasing amount of attention.With contributions from leading experts in biology, biotechnology, and chemistry of carotenoid research, this volume discusses the biological functions of carotenoids such as astaxanthin, β-cryptoxanthin, and fucoxanthin, in addition to paprika carotenoids, capsanthin, and capsorubin. It also reveals the technologies behind the commercial production of some functional carotenoids.The book is targeted for academic and industrial readers in biology, biotechnology, nutrient physiology and related fields.Table of Contents​

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Book SynopsisThis book presents fundamental and practical information on food chemistry. Using 2-D barcodes, it illustrates the specific reactions and potential transformation mechanisms of food constituents during various manufacturing and storage processes, and each chapter features teaching activities, such as questions and answers, and discussions. Further, it describes various local practices and improvements in Asia. Divided into 12 chapters covering individual nutrients and components, including water, proteins, carbohydrates, lipids, vitamins, minerals, enzymes, pigments, flavoring substances, additives, and harmful constituents, it addresses their food chemistry, as well as their transformations during manufacturing processes, and typical or advanced treatments to improve food quality and safety. This book helps college students to gain a basic understanding of nutrients and food components, to discover and implement the practical industrial guidelines, and also to learn the latest developments in food chemistry.Table of ContentsIntroduction.Water.- Proteins.- Carbohydrates.- Lipids.- Vitamins.- Minerals.- Enzyme.- Pigments.- Food Flavor Substances.- Food additives.- Harmful Constituents.

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Book SynopsisThis edited book is focused on antioxidant compounds and their biosynthesis, up-regulation, mechanism of action for selective bioactivity, targeted role and the advancement of their bioactive potential during plant-microbe interaction and other stress conditions. This book also emphasizes on the role of antioxidants in recruiting beneficial microbes in plant surroundings. Antioxidants have multiple biological roles in plants especially in the signalling pathway. These compounds are secondary metabolites produced besides the primary biosynthetic pathway and are associated with growth and development. Besides they also have special role to play during oxidative stress produced via abiotic stimulants or pathogen attack. This understanding of the biosynthesis, signaling and function of antioxidant compounds in plants during stress condition is helpful in restoring plant ecosystem productivity and improve plant responses to a wide range of stress conditions. This book is a useful compilation for researchers and academicians in botany, plant physiology, plant biochemistry and stress physiology. Also the book serves as reading material for undergraduate and graduate students of environmental sciences, agricultural sciences and other plant science courses.Table of ContentsChapter 1. Antioxidants in Plant-Microbe Interaction.- Chapter 2. Plant microbe symbiosis led synthesis of bioactive compounds.- Chapter 3. Plant-Rhizobacteria communications with antioxidant system.- Chapter 4. Association of Non-Enzymatic Antioxidants in Plant Holobiont.- 5. Carotenoids and flavonoids in plant stress management.- Chapter 6. PGPR-the redeemer of rice from abiotic stress.- Chapter 7. Impact of PGPR on plant health and antioxidant enzymes under water stress conditions.- Chapter 8. Rhizospheric plant-microbe interactions releasing antioxidants and phytostimulating compounds in polluted agroecosystems.- Chapter 9. Role of Antioxidant in plant and microbes based remediation of metal stress.- Chapter 10. Amelioration of drought stress through PGPR mediated regulation of Antioxidant defensive machinery.- Chapter 11. Potential of plant growth promoting microbes in disease reduction by influencing the antioxidant enzymes of medicinal and spice plants.- Chapter 12. Antioxidants in spices: a review of the antioxidant components and properties of some common african spices and their role in human nutrition and plant-microbe interactions.- Chapter 13. Impact of plant growth promoting microbes (PGPM) in plant disease management by inducing non-enzymatic antioxidant.- Chapter 14. Antioxidants as modulators of plant defense against soil-borne fungal pathogens upon microbial interaction.- Chapter 15. A Promising Approach of Managing Seed Borne Pathogens through Plant Growth Promoting Microbes.- Chapter 16. Antioxidant Potential of Plant Growth Promoting Rhizobacteria (PGPR) in Agricultural Crops Infected with Root Knot Nematodes.- Chapter 17. Disease management and the role of antioxidants in combating plant pathogens upon PGPR inoculation with special reference to legumes.- Chapter 18. Rhizobacters as remedy of stress tolerance in potato plant.- Chapter 19. Secondary metabolites, boon for plants; their role in defence mechanism and antioxidant activity of Anthocephalus cadamba.- Chapter 20. Role of PGPR in conferring drought stress tolerance in Rice.- Chapter 21. Fenugreek-rhizobium symbiosis and Flavonoids under stress condition.- Chapter 22. Datura stramonium: An overview of its antioxidant system for plant benefits.- Chapter 23. PGPR mediated regulation of antioxidants: Prospects for abiotic stress management in plants.- Chapter 24. Prospects of PGPRs mediated antioxidants and S and P metabolism in plants under drought stress.- Chapter 25. Prominence of Antioxidant Potential of Plants and its induction by interaction with microorganisms.- Chapter 26. Bio-molecular painstaking utilization and assimilation of phosphorus under indigent stage in agricultural crops.- Chapter 27. Plant Antioxidant System Regulates Communication Under Abiotic Stress For Enhanced Plant Productivity.- Chapter 28. Nematophagous fungi in antioxidant mediated defence against plant parasitic nematodes.- Chapter 29. Biopriming and Nanopriming: Green Revolution Wings to Increase Plant Yield, Growth and Development under Stress Condition and Forward Dimensions.

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Book SynopsisThis volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags. It also discusses techniques and factors that affect the amine and biotin-streptavidin coupling and methods to optimize the interactions. Next, it describes fragment preparation for screening in SPR and presents methods to calculate equilibrium dissociation constant (KD) and ligand efficiency (LE). It reviews techniques of next-generation injections that improve the efficiency of the characterization process over traditional SPR by determining the kinetics and affinity in a single step. Finally, the book elucidates a comprehensive yet representative description of challenges associated with the molecular interaction of proteins using SPR.Table of ContentsChapter 1_Introduction to SPR.- Chapter 2_ Principle of SPR (OneStep).- Chapter 3_Identification of Fragment library.- Chapter 4_Preparation of protein with Histidine Tag.- Chapter 5_Preparation of protein with Biotin Tag.- Chapter 6_Protein stability using Thermal Shift Assay (TSA) - pH.- Chapter 7_Protein stability using Thermal Shift Assay (TSA) – DMSO.- Chapter 8_Preparation of Sensor.- Chapter 9_ Optimization and validation of Amine coupling.- Chapter 10_ Optimization and validation of Biotin-strepavidin coupling.- Chapter 11_ Preparation of Fragment for Screening in SPR.- Chapter 12_ Screening of Fragment in SPR.- Chapter 13_ Analysis of Data.- Chapter 14_ Identification and arraying of HITS.- Chapter 15_ Confirmation of Hits – Kinetics (kon, koff, KD, LE).

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Book SynopsisBasic principles of applied life sciences such as recombinant DNA technology is used in most life sciences industries marketing bio-formulations for designing more effective protein-based drugs, such as erythropoietin and fast-acting insulin etc. In recent times genetically engineered host cells from mammal, animal and plants are also being used in life sciences industries to manufacture biologics. This book discusses the most basic as well advanced issues on biological products for successfully managing a life sciences industry. It elucidates the life cycle of biological molecules, right from the conceptual development of different types of biopolymers, and their subsequent transfer from the conical flasks in laboratory to life sciences industries for large scale production and marketing. It focuses on sustainable longevity in the life cycle of commercial biopolymers. Cumulative facts and figures in this volume would immensely help in inspiring life sciences industry promoters to monitor value chain transfer process of biologics for better profitability. Additionally, it would serve as a perusal document for the students and researchers interested in entrepreneurial ventures or having their own start-up projects for the commercialization of biologics. Table of ContentsChapter 1. Prelusion Significance of Livestock.- Chapter 2. Mass Balance concept in Livestock Farming.- Chapter 3. Goat and Sheep Farming.- Chapter 4. Cattle and Buffaloes Farming.- Chapter 5. Factors Influencing Livestock Way of Life.- Chapter 6. Sustainable Livestock Farming.- Chapter 7. Conceptual Development of Livestock Supply Chain Management.

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Book SynopsisThis book highlights recently discovered aspects of “middle-size molecules,” focusing on (1) their unique bio-functions on the basis of derivatives and conjugates of natural products, saccharides, peptides, and nucleotides; (2) the synthesis of structurally complex natural products; (3) special synthetic methods for π-conjugated functional molecules; and (4) novel synthetic methods using flow chemistry. Given its scope, the book is of interest to industrial researchers and graduate students in the fields of organic chemistry, medicinal chemistry, and materials science. Table of ContentsPart I: Development of bio-functional middle molecules.- 1. Introduction.- 2. Total Synthesis, Biological Evaluation and 3D Structural Analysis of Cyclodepsipeptide Natural Products.- 3. Development of the Middle-size Molecules for Alkylation to Higher-Order Structures of Nucleic Acids.- 4. In Situ Synthesis of Glycoconjugates on Cell Surface: Selective Cell Imaging Using Low-Affinity Glycan Ligands.- 5. Assembled mid-sized agents that control intracellular protein-protein interactions.- 6. Macrocyclic mid-sized peptides with new chemical modalities.- Part II: Achievement of highly efficient synthesis of bioactive middle molecules.- 7. Enantioselective Total Synthesis of Cotylenin.- 8. Flow Chemistry for the Construction of Polycyclic Skeleton.- 9. Electrochemical Synthesis of Oligosaccharides as Middle-sized Molecules.- 10. Efficient Synthesis of Biologically Active Peptides based on Micro-flow Amide Bond Formation.- 11. Design and Concise De Novo Synthesis of Artemisinin Analogs.

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Book SynopsisThis book on potassium in abiotic stress tolerance deals with the ongoing trend in increasing abiotic stresses and interlinked issues food security. As mineral nutrient potassium holds an important place in agriculture and is involved in various physiological and biochemical processes. It takes part in protein synthesis, carbohydrate metabolism, enzyme activation, cation-anion balance, osmoregulation, water movement, energy transfer, and regulates stomata and photosynthesis. Potassium plays an important role as abiotic stress buster. This book will deal with potassium relevance to plant functions and adaptations, range of its biological functions, role of potassium in abiotic stress tolerance, analyses of mechanisms responsible for perception and signal transduction of potassium under abiotic stress, critical evaluation of and cross-talks on nutrients and phytohormones signaling pathways under optimal and stressful conditions, and interaction of potassium with other nutrients for abiotic stress tolerance. This book will be of interest to teachers, researchers, scientists working on abiotic stresses. Also the book serves as additional reading material for undergraduate and graduate students of agriculture, forestry, ecology, and environmental sciences. National and international agricultural scientists, policy makers will also find this to be a useful read.Table of ContentsChapter 1. Role of Potassium in Plant Photosynthesis, Transport, Growth and Yield.- Chapter 2. Potassium Role in Plants Response to Abiotic Stresses. Chapter 3. Molecular Approaches to Potassium Uptake and Cellular Homeostasis in Plants under Abiotic Stress.- Chapter 4. Soil Potassium Availability and Role of Microorganisms in Influencing Potassium Availability to Plants.- Chapter 5. Crosstalk of Potassium and Phytohormones under Abiotic Stress.- Chapter 6. Potassium (K+) Regulation by Phytohormones under Abiotic Stress.- Chapter 7. Role of Potassium in Drought Adaptation: Insights into Physiological and Biochemical Characteristics of Plants.- Chapter 8. Role of Potassium in Heavy Metal Stress.- Chapter 9. Salt Stress Alleviation Strategies to Maintain K+ Homeostasis in Plants.- Chapter 10. Potassium ion Homeostasis, Signaling and Changes in Transcriptomes and Metabolomes Enduring Salinity Stress.- Chapter 11. Potassium: A Potent Modulator of Plant Responses under Changing Environment.- Chapter 12. An Overview of Potassium in Abiotic Stress: Emphasis on Potassium Transporters and Molecular Mechanism.- Chapter 13. Nitric Oxide Synthesis Affects Potassium and Nitrogen Homeostasis in Plants for Salt Tolerance.

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Book SynopsisThis book is a comprehensive account of recent advances in the endophytic research. It covers recent perspective of endophytic research, molecular diversity, bioprospecting of novel genes using high throughput molecular techniques, and most importantly application of endophytes in practicing sustainable agriculture. Endophytic micro-organisms are mysterious living component associated mutually with plant roots and soil microbes. Various endophytic bacteria have attracted considerable attention for their ability to promote plant growth through direct mechanisms or by acting as biocontrol agents. Endophytes also find use in biocontrol, medicine, agriculture and food industry. This is a useful reading for the student of agriculture, environmental microbiology and biotechnology.Table of Contents1 Endophytic Bacteria: Application against biotic and abiotic stresses and Plant Health Improvements for Sustainable Agriculture.- 2 Endophytic Bacteria: Mitigating Abiotic Stress from Inside.- 3 Diversity and bioactive potential of endophytic bacteria from high-value medicinal plants.- 4 Plant Growth Promoting Rhizobacteria (PGPR) Assisted Phytoremediation of Contaminated Soils.- 5 Endophytic Bacteria: Role in Phosphorous Solubilization.- 6 Endophytes of Medicinal plants: Diversity and Bioactivity.- 7 Title: Biotechnological applications of Bacterial Endophytes.- 8 Genetic Basis of Fungal Endophytic Bioactive Compounds Synthesis, Modulation, and Their Biotechnological Application.- 9 Endophytic bacteria for plant growth promotion.- 10 Bacterial Endophytes and Bio-nanotechnology.- 11 Role of Endophyte Metabolites in Plant Protection and other Metabolic Activities.- 12 Role of Bacterial Endophytes in the Promotion of Plant Growth.- 13 Bacterial endophytes and abiotic stress mitigation.

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Book SynopsisThis book presents the state of the skill of understanding brassinosteroids (BRs) signaling plus crosstalk with phytohormone and their association in plant adaptation to abiotic stresses comprising physiological, biochemical, and molecular developments. Due to progressively adverse environmental conditions and scarce natural resources, high-efficient crops have become more important than ever. For the successful improvement of stress-tolerant plants, it is vital to understand the precise signaling appliances that plants practice to abide stresses as well as how much these mechanisms are convinced by phytohormone. However, it is also debatable on which step plants can attain brassinosteroids (BRs) signaling from an evolutionary viewpoint. BRs are involved in modulating a large array of important functions throughout a plant’s life cycles. BRs are considered as one of the most important plant steroidal hormones that show a varied role in observing a wide range of developmental practices in plants. Our grip on brassinosteroids signaling has quickly extended over the past two decades, owing in part to the isolation of the constituents intricate in the signal transduction trail. The book proposes a useful guide for plant researchers and graduate students in connected areas. Table of ContentsChapter 1Signal transduction of brassinosteroids under abiotic StressesChapter 2Cross talk between brassinosteroids and nitric oxide regulate plant improvement during abiotic stressChapter 3Brassinosteroids and strigolactones signaling in plantsChapter 4Interaction between brassinosteroids and hydrogen peroxide networking signal molecules in plantsChapter 5The production of high-value secondary metabolites through hairy root transformation in the presence of brassinosteroidsChapter 6Role of brassinosteroids in protein folding under high temperature stressChapter 7Mechanism associated with brassinosteroids cross talk with gibberellic acid in plantsChapter 8Brassinosteroids and ethylene mediated cross talk in plant growth and developmentChapter 9Interplay of brassinosteroids and auxin for understanding of signaling pathwayChapter 10Brassinosteroids cross talk with ABA under stress condition.Chapter 11Cross talk between brassinosteroids and cytokinins in relation to plant growth and developmentsChapter 12Plant proteomics and metabolomics investigations in regulation of brassinosteroidsChapter 13Mechanism associated with brassinosteroids mediated detoxification of pesticides in field grown plantsChapter 14Role of brassinosteroids and its crosstalk with other phytohormone in plant responses to heavy metal stressChapter 15Glyphosate: is brassinosteroids application a remedy?Chapter 16Molecular mechanism of brassinosteroids in boosting crop yield

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Book SynopsisThis book comprehensively reviews the association of homocysteine metabolism with the etiology of various human disorders. The well-defined chapters embedded the central and peripheral effects of homocysteine metabolism intricately related with cardiovascular, neurodegenerative, metabolic, and autoimmune disorders. Further, it discusses the mechanisms of perturbance of cellular proteostasis by elevated homocysteine levels and provides a comprehensive account of pathophysiological consequences and clinical implications of homocysteine-containing proteins. The book also reviews association of genetic variants of homocysteine metabolic genes with type 2 diabetes mellitus and obesity. It also describes the molecular mechanism of hyperhomocysteinemia in the negative/feedback regulation of neural stem cell proliferation and alterations in DNA methylation. Taken together, it summarizes the mechanisms of hyper homocysteinemia-induced endothelial dysfunction and physiological functions of hydrogen sulfide as the protective agent.Table of ContentsChapter 1_Overview of homocysteine. –Chapter 2_Homocysteine and Folic acid metabolism. –Chapter 3_ Hyper-homocysteinemia and nutrition. –Chapter 4_ Metabolic disorders and Homocysteine. –Chapter 5_Bone Health and homocysteine. –Chapter 6_Biofortification, homocysteine and nutrition. –Chapter 7_ Homocysteinemia and autoimmune diseases. –Chapter 8_Homocysteine metabolism pathway genes in risk of type-2 diabetes mellitus. –Chapter 9_Role of homocysteine metabolism in cardiovascular diseases (CVD). –Chapter 10_ Homocysteine and thyroidism. –Chapter 11_Homocysteine metabolism and cancer. –Chapter 12_Stem cell and Homocysteine metabolism in treatment and management of diseases. –Chapter 13_Homocysteine in Nutritional Determinants; Genetic Susceptibility to Neural Tube Defect (NTD). –Chapter 14_Homocysteine metabolism dependent computational drug development: current status and future perspectives. –Chapter 15_Hyper-homocysteinemia mediated neuro-inflammation and neurodegenerative disorders. –Chapter 16_Homocysteinemia and viral infection (Covid-19). ​

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Book SynopsisThe second volume of the Biomedical translational research discusses advancements in biomedical research for understanding the pathophysiology of various diseases towards improving diagnosis and treatment. It presents the integration of molecular-based technologies, clinical genomics, and medical informatics to improve diagnostic and treatment strategies. Further, the book reviews molecular genomics approaches for diagnosis and managing tuberculosis. It also covers the innovative strategies for cancer treatment through targeting metabolic pathways, tumor microenvironment, cancer stem cells, and immune cells. It also illuminates novel strategies for heart failure diagnosis and therapeutic approaches for the treatment of heart failure. It discusses improvements in translational research for discovery of new diagnostic tests, identifying novel biomarkers and drugable targets, and predicting optimal treatments based on understanding the underlying molecular basis of the disease. Lastly, it reviews the preclinical models of restenosis and their application and limitation in the evaluation of device-based interventional technologies for the treatment of coronary artery diseases.Table of Contents Application of clinical genomics in diagnosis and disease treatment Exosomes as novel biomarkers of disease Membrane Lipids: Implication for Diseases and Membrane Trafficking Human natural killer cells and their cytotoxic capacity Molecular genomic approaches for diagnosing and managing tuberculosis Exploring the origin of cancer with stem cell biology Therapeutic potential of skin stem cells in vitiligo Clinical applications of cancer stem cells Immuno therapeutic approaches for cancer treatment Future of uro-oncology Prostate Cancer: Circadian Gene Regulated Malignancy Role of adenosine pathway in cancer Immune modulation and VEGF Modulating tumour microenvironment in the treatment of cancer The endosymbiont role of mitochondria in cancer progression and therapy Interplay of cancer associated fibroblasts and tumour associated macrophages in metastasis Chimeric antigen receptor therapy : A cutting edge therapy for multiple myeloma The future therapies for heart failure Futuristic experimental studies on heart failure and ischemic heart diseases AGE-PAGE Stress in the pathophysiology of atherosclerosis and its treatment Genetics of Left Ventricular Dysfunction in Coronary Artery Disease Gene editing in liver disease Interrupting crystal to calculus conversion : The future of renal stone research Preclinical models of intimal hyperplastic and restenosis for the development of novel therapies Nanoparticles in counteracting multidrug resistant bacterial Rapid eye movement sleep and dream sleep

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Book SynopsisThis book discusses the potential roles of miRNA as a crucial regulator in cancer biology. It examines the regulation of drug resistance by miRNAs in cancers and the mechanism of their deregulated expression. The book discusses the role and molecular mechanism of miRNA in regulating cellular proliferation and cell cycle. It analyses circulating miRNAs as biomarkers for cancer diagnosis and prognosis. It also analyzes the role of miRNA as a modulator of the development and function of tumor-associated immune cells. It explores the cross-talk between miRNA and reactive oxygen species (ROS) in pathogenesis, cancer therapeutic tolerance, and resistance. It provides insights into the role of miRNA in cancer angiogenesis, metastasis and describes strategies and associated challenges of miRNA-based therapies.Table of ContentsChapter 1. Introduction to microRNAs and cancers.- Chapter 2. microRNAs and cancer signaling pathways.- Chapter 3. Role of microRNAs in cell growth proliferation and tumorigenesis.- Chapter 4. Impact of microRNAs in cell adhesion and tumor angiogenesis.- Chapter 5. Modulation of oxidative stress by microRNAs in cancers.- Chapter 6. microRNAs targeting tumor microenvironment and immune modulation.- Chapter 7. Circulating miRNA in prognosis, diagnosis and their role in controlling the fate of cancer stem cells.- Chapter 8. The role of miRNAs in the regulation of drug resistance among different cancers.- Chapter 9. The mechanisms of miRNA deregulation in cancer outbreak.- Chapter 10. Bioinformatic profiling of miRNAs in Cancers.

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Book SynopsisThis book provides a methodical compilation of deriving composites from the hidden treasure of the aquatic world.Continuous and rapid progress in the composite industries have increased the demand for resilient, economically viable, and sustainable composite materials having enhanced mechanical , thermal and electrical properties which better suits there respective applications.If the materials/organisms used for the production or conversion of composites are renewable, degradable and easily and abundantly available then it gives great opportunity to the researchers to work on different options or processes to make them a viable technology. This work describes the organisms and materials present in the aquatic environment for the production of composite materials. Elaborating the versatile green expedients and their potential applications in the field of composites. Since, growing ecological and environmental consciousness has driven efforts for development of new innovative materials for various end-use applications.Therefore the LCA an circular bio-economy will be discussed to be efficient and sustainable. This book is ideal for the students, academicians, researchers and industry players. It also cover the present scenario, applications and future perspectives of composites derived from aquatic organisms. This compiled book features chapters that discuss the conversion of different materials and organisms present in aquatic environment to composite materials like micro-algae, seaweeds, chitosan, collagen, agar cyanobacteria etc.in a viable manner.Table of ContentsOverview of composites from the aquatic environment.- LCA of microalgae biopolymer composites.- Microalgae based biopolymer composites: Role in circular bioeconomy.- Recent development in water hyacinth fiber composite and its application.- Characterization of water hyacinth biopolymer composites.- Chitosan (sea shell) based composites:Recent trends and future scope.- Preparation and characterization of chitosan biocomposites.- Development and characterization of agar biopolymer based composites.- Performance of Fish scale composites.- Development and characterization of collagen composites.- Recent advances in composites from seaweeds.- Nipah (nypa fruticans) palm fibre extraction process for nipah fibre reinforced polymer composites.- Starch/carrageenan blend based biocomposite as packaging materials.

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Book SynopsisThe book presents a systematic and detailed introduction on starch debranching enzymes concerning the classification, biochemical properties, features on sequences and structures, enzyme engineering, production, and current applications. All relevant contents are organized to focus on characteristics, productions and industrial applications of the starch debranching enzymes. It is purposed to deepen the understandings on the pre-existing researches, developments, and bottlenecks, and also to discuss the research hotspots and application perspectives of starch debranching enzymes. The book is written for researchers, professional/practitioners and graduate students in the field of enzymology, microbiology, and food science etc.Table of ContentsChapter 1. An Overview on Starch Processing and Key Enzymes.- Chapter 2. Classification and Enzyme Properties of Starch Debranching Enzymes.- Chapter 3. Sequence, Structure, and Engineering of Microbial Starch Debranching Enzymes.- Chapter 4. Production and the Applications in Preparation of Branched Sugar Products of Starch-Debranching Enzymes.- Chapter 5. Recombinant Expression of Starch Debranching Enzymes in Escherichia coli.- Chapter 6. Production of Starch Debranching Enzymes in Bacillus Strains. Chapter 7. Applications of Starch Debranching Enzymes in Starch Processing

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Book SynopsisThis book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods, and provides concise descriptions of the effective applications of these methods.Table of ContentsFD-LC-MS/MS. 2-D DIGE. Mass Spectrometry Utilizing ICAT. Proteomic Analyses of Post-translational Modifications. Cardiovascular Proteomic Analysis. Proteome in Neurodegenerative Diseases. Liver Disease-Related Proteome. Respiratory Disease Related Proteome. Renal Disease-Related Proteome. Aging-Related Proteome. Phosphoproteomics of Tumor Cell Lines. HCV Infection and Mitochondria Proteomics. Infectious Disease by SELDI. Quantitative Proteomic Analysis of HIV Infection.

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Book SynopsisThis book describes the design and the use of bioluminescent biosensors. It introduces beginners and experienced researchers starting in the microbiological biosensor domain to the practical aspects of building a luminescent microbial biosensor. It is also a source of information about other applications that use microbial cells. Each chapter focuses as far as possible on the technological conception of the presented biosensor with a clear demonstration of the issues in the design and how to reach the proof of concept. The book is divided into three practical sections facilitating the reader to easily access the information.Table of ContentsLuminescent Biosensors with Free Suspended Cells. Fully Automated Fluidic Analyzer for Food Quality Assessment Using Bioluminescent Biosensors. Technological Design of Optical Bacterial Biosensors for the Online Environmental Monitoring of Water Pollutants. Luminescent Biosensors with Immobilized Cells. Fiber Optic Biosensors for Environmental Monitoring. Non-Fiber Optic Bioluminescent Biosensors. Biosensor with Immobilized Cells and Lensless Imaging Detection Luca CeveniniaA biosensor with Genetically Modified Bacteria Immobilized on a Fiber and a Glass Slide. Development and Characterization of a Living-Cell Bioluminescent Bioreporter Integrated Circuit (BBIC). BOD Sensor with Immobilized Luminous Cells on ChipBioluminescent Whole-Cell Sensors: Integration of Bacterial Reporter Cells onto Hardware Platforms. Luminescent Biosensors with Bioluminescent Sporulated Cells ("Sporosensor"). Luminescent Biosensing Systems Based on Genetically Engineered Spore-Forming Bacteria.

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Book SynopsisUnderstanding Enzymes: Function, Design, Engineering, and Analysis focuses on the understanding of enzyme function and optimization gained in the past decade, past enzyme function analysis, enzyme engineering, and growing insights from the simulation work and nanotechnology measurement of enzymes in action in vitro or in silico. The book also presents new insights into the mechanistic function and understanding of enzyme reactions, as well as touching upon structural characteristics, including X-ray and nuclear magnetic resonance (NMR) structural methods. A major focus of the book is enzyme molecules’ dependency on dynamic and biophysical environmental impacts on their function in ensembles as well as single molecules. A wide range of readers, including academics, professionals, PhD and master’s students, industry experts, and chemists, will immensely benefit from this exclusive book.Table of ContentsA practical guide to the quantitative analysis of engineered enzymes. Protein Conformational Motions - Enzyme Catalysis. Enzymology meets Nanotechnology: Single-molecule methods for observing enzyme kinetics in real time. Interfacial enzyme function visualized using neutron, x-ray and light scattering methods. Folding dynamics and structural basis of the enzyme mechanism of ubiquitin C-terminal hydroylases. Stabilisation of Enzymes by metal binding: Structures of Alkalophilic Bacillus proteinases and analysis of the second metal binding site of subtilases. Structure and Functional Roles of Surface Binding Sites in Amylolytic Enzymes. Interfacial Enzymes and their Interactions with Surfaces: Molecular Simulation Studies. Sequence, structure, function: What we learn from analyzing protein families. Bioinformatic analysis of protein families to select function-related variable positions. Decoding life secrets in sequences by chemicals. Role of tunnels and gates in enzymatic catalysis. Molecule descriptors for the structureal analysis of enzyme active sites. Hydration effects on enzyme properties in nonaqueous media analysed by MD simulations. Understanding esterase and amidase reaction specificities by molecular modelling. Towards new non-natural TIM-barrel enzymes using computational design and directed evolution approaches. Handling the Numbers Problem in Directed Evolution. Hints from Nature: Metagenomics in Enzyme Engineering. A functional and structural assessment of circularly permuted Bacillus circulans xylanase and Candida antarctica lipase B. Ancestral Reconstruction in Enzymes. High throughput screening or selection methods for evolutionary enzyme engineering. Nanoscale Enzyme Screening Technologies. Computational Enzyme Engineering: Activity Screening using Quantum Chemistry. In silico screening of enzyme variants by molecular dynamics simulation. Kinetic and thermodynamic stability of variant enzymes.

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Book SynopsisChapter 1 Photosynthetic microbes Evolution, Classification and Physiology.- Chapter 2 Structural and functional dynamics of microbial photosystem complexes.- Chapter 3 Photosynthetic CO2 fixation in unicellular microbes.- Chapter 4 Techniques in photosynthetic physiology and cultivation of photosynthetic microbes.- Chapter 5 Approaches in wastewater utilization using photosynthetic microbes.- Chapter 6 Microalgae photosynthesis for nutrient recovery and value addition.- Chapter 7 Bacterial Photosynthesis for nutrient recovery and value Addition.- Chapter 8 Advent of genetic and metabolic engineering in improving microbial photosynthesis.- Chapter 9 Industrial symbiosis of photosynthetic microbial biorefineries for circular economy.- Chapter 10 Artificial photosynthesis nexus of photon energy and bioreactors.

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Book SynopsisThe book is divided into six major sections 1) Enzyme Catalysis A Perspective, 2) Enzyme Kinetic Practice and Measurements, 3) Elucidation of Kinetic Mechanisms, 4) Chemical Mechanisms and Catalysis, 5) Exploiting Enzymes, and 6) An end piece on Frontiers in Enzymology.

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Book Synopsis1. Introduction to Inulin.- 2. Extraction and Purification of Inulin.- 3. Inulin: A Versatile Polymer for Drug Delivery System.- 4. Inulin as Stabilizer.- 5. Inulin as a Coating Agent.- 6. Inulin as a Prebiotic and its Effect on Gut Microbiota.- 7. Inulin as a Natural Ingredient in Cosmetics and Personal Care Products.- 8. Inulin as a Pharmaceutical Excipient.- 9. Toxicity and Safety Aspects of Inulin.- 10. Role of Inulin in the Management of Metabolic Disorders.- 11. Inulin and Gastrointestinal Disorders.- 12. Inulin: A Versatile Polymer in Colon Drug-targeting.- 13. Regulatory Aspects of Inulin-Based Pharmaceutical Products.- 14. Therapeutic Role of Inulin in Disease Management.- 15. Future Prospects in Inulin Research.- 16. The Potential of Inulin to Revolutionize Pharmaceutical Industries.

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Book Synopsis1. Natural products for antibacterial infections in current scenario.- 2. Natural products in management of MDR.- 3. Natural Product-Based Treatment of Urinary Tract Infections.- 4. Natural Product-Based Treatment for Tuberculosis.- 5. Natural Product-Based Treatment for Leprosy.- 6. Natural Product-Based Treatment of Ear infection.- 7. Natural Product-Based Treatment for Skin Infections.- 8. Natural Product-Based Treatment for Gastritis.- 9. Natural Product-Based Treatment for Food Poisoning.- 10. Multi drugs resistance: Concepts, mechanisms and recent advances.- 11. Approaches of Bioinformatics in Antibacterial Drug Development.- 12. Antibiotic Drug Development Methods.

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