Insects (entomology) Books

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Book SynopsisThis title is a much needed update of Barbosa''s self-published Manual of Basic Techniques in Insect Histology. It is a laboratory manual of ''traditional'' and ''modern'' insect histology techniques, completely revised using cutting-edge methodology carried out today and includes new immunohistochemical techniques not previously looked at. Insect Histology is designed as a resource for student and professional researchers, in academia and industry, who require basic information on the procedures that are essential for the histological display of the tissues of insects and related organisms.Trade Review“A very useful book to have in your library.” (British Journal of Entomology & Natural History, 1 March 2015) Table of ContentsPreface ix Acknowledgements xi Introduction xiii About the companion website xiv 1 Problems of sclerotized chitin: Softening insect cuticle 1 1.1 Introduction 1 1.2 General Methods 3 1.3 Preparations of insect eggs 14 1.4 Double Embedding Techniques 16 References 19 2 Fixation 21 2.1 Introduction 21 2.2 Aldehyde based fixatives 21 2.3 Protein denaturing 30 2.4 Picric acid based 33 2.5 Mercuric chloride based 37 2.6 SEM/TEM 40 2.7 Other 46 References 51 3 Dehydrating, clearing, and embedding 54 3.1 Dehydration 54 3.2 Clearing 60 3.3 Embedding General 65 3.4 Embedding – Ester Wax 73 3.5 Embedding – Methacrylate 74 References 77 4 Staining 79 4.1 Single-contrast staining – Carmines 81 4.2 Single contrast staining – Nuclear Stains 83 4.3 Single contrast staining – General Stains 86 4.4 Single contrast staining – Golgi 89 4.5 Single contrast staining – Eggs 89 4.6 Single contrast staining – Silver Stains 90 4.7 Polychrome staining techniques – General 92 4.8 Polychrome staining – Brain/Nerve 102 4.9 Polychrome staining – blood 103 4.10 Single contrast procedures for chitinous material 105 4.11 Polychrome staining procedures for chitinous material 106 4.12 Polychrome staining for chitinous material – KOH 110 4.13 Polychrome staining for chitinous material – Differential staining of Individual Organs 111 4.14 Staining of specific tissues 113 4.15 Two dye combinations 114 References 117 5 Immunohistochemical techniques 119 5.1 Introduction 119 5.2 General immunostaining techniques 127 5.3 Immunolabeling of samples for Transmission Electron Microscopy (TEM) 135 5.4 Proliferation assays 140 5.5 Methods to detect specific proteins 142 References 144 6 Use of genetic markers in insect histology 146 6.1 Introduction 146 6.2 Inducible genetic markers 149 6.3 Mosaic gene expression 156 6.4 Fluorescent markers for live imaging and kinetic microscopy 165 References 169 7 Fluorescence 171 7.1 Introduction 171 References 192 8 Mounting 194 8.1 Introduction 194 References 206 9 Preparation of whole mounts 208 9.1 Introduction 208 References 229 10 Preparation of whole mounts for staining 231 10.1 Introduction 231 10.2 Detection of NAPDHd 237 10.3 SEM 238 10.4 In situ hybridization 240 References 244 11 Preparation of genitalia, mouthparts and other body parts 246 References 256 12 Preparation of chromosomes 258 References 288 13 Preparation of other specific insect organs and tissues 290 13.1 Introduction 290 References 323 Appendix Dissecting fluids and saline solutions 325 Index 333

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Book SynopsisPhil Lester’s first book, The Vulgar Wasp, was about one of the world’s most hated insects. His second is about just the opposite - the honey bee, arguably one of our best-loved six-legged creatures. People have revered honey bees for centuries. Today we celebrate them with toys, postage stamps and campaigns to raise awareness; we dress up in large bee suits to protest the use of pesticides; we’ve even sent bees into space and watched as they adjusted to microgravity.Bees are one of the world’s most efficient pollinators. Their work is vital to the success of many food crops, and hence to the world’s economy. So we need to take seriously any threats to their health - including parasites, pathogens, predators and pesticides - and, guided by evidence at every turn, find a way to minimise harm and keep bees thriving. As Healthy Bee, Sick Bee shows, this is no small task.In this book, entomologist Phil Lester explores the wonderfully complex and sometimes brutally efficient life history of honey bees, and the problems they face in New Zealand and around the globe. What causes a beehive to collapse? Are pesticides as big a problem as they appear? What can we do to improve the health?

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Book SynopsisThe worksheets and activities in Insects, Science and Society: Laboratory Manual are designed to help bring students into a hands-on laboratory-style setting while maintaining a structure for entry level study. By understanding the characteristics associated with arthropods and the "Super Seven" largest insect orders as well as techniques for exploration, identification, and collection of arthropods students can make connections to the diversity of life that encompasses us every day in nearly every setting. To know something about entomology is to know something about the world around us.Table of Contents Worksheets Introduction to Arthropods and Insects Dragonflies and Damselflies True Bugs: Hoppers, Cicadas, Aphids, Whiteflies, and Scales Beetles Grasshoppers, Crickets, and Katydids True Flies Bees, Wasps, and Ants Moths and Butterflies Activities External Morphology Scientific Method Isopod Rodeo Isopod Rodeo Lab Report: Hints, Tips, and Tricks Isopod Rodeo Lab Report Insect Collecting Techniques Insect Collection Project Insect Identification: Use the Simplified Dichotomous Key Insect Identification: Simplified Dichotomous Key Making a Dichotomous Key Grasshopper Internal Dissection Generalized Insect Alimentary Canal, Indicating the Three Primary Regions Population Dynamics Biological Control with Predators Food Web

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