Cellular biology (cytology) Books
Taylor & Francis Ltd Gene Targeting and Embryonic Stem Cells
Book SynopsisGene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.Table of Contents1. An Overview of Gene Targeting and Stem Cells 2. Advances in Gene Targeting in Murine Embryonic Stem Cells 3. Nuclear Transfer with Murine ES Cells 4. Gene Targeting in Sheep 5. RNA Interference 6. Human Embryonal Carcinoma Cells: Surrogate Tools in the Study of Human Embryonic Stem Cells 7. Isolation of Human ES Cells 8. In Vitro Culture and Differentiation of Human Embryonic Stem Cells 9. Transfection of Human Embryonic Stem Cells
£63.64
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Morphogenesis and Pathogenicity in Fungi
Book SynopsisInfectious fungal diseases continue to take their toll in terms of human suffering and enormous economic losses. Invasive infections by opportunistic fungal pathogens are a major cause of morbidity and mortality in immuno-compromised individuals. At the same time, plant pathogenic fungi have devastating effects on crop production and human health. New strategies for antifungal control are required to meet the challenges posed by these agents, and such approaches can only be developed through the identification of novel biochemical and molecular targets. However, in contrast to bacterial pathogens, fungi display a wealth of “lifestyles” and modes of infection. This diversity makes it extremely difficult to identify individual, evolutionarily conserved virulence determinants and represents a major stumbling block in the search for common antifungal targets. In order to activate the infection programme, all fungal pathogens must undergo appropriate developmental transitions that involve cellular differentiation and the introduction of a new morphogenetic programme. How growth, cell cycle progression and morphogenesis are co-ordinately regulated during development has been an active area of research in fungal model systems such as budding and fission yeast. By contrast, we have only limited knowledge of how these developmental processes shape fungal pathogenicity, or of the role of the cell cycle and morphogenesis regulators as true virulence factors. This book combines state-of-the-art expertise from diverse pathogen model systems to update our current understanding of the regulation of fungal morphogenesis as a key determinant of pathogenicity in fungi. Trade ReviewFrom the reviews:“This book describes the molecular basis of morphogenesis and pathogenicity in the fungi and highlights new approaches. … All the chapters are well organized and each chapter contains an introduction at the beginning and a conclusion at the last that can be very useful for the reader. … This book is very important to scientists, researchers and teachers as well as students who are interested in fungal morphogenesis and pathogenicity. Therefore this book should be available in all schools, research laboratories, community and university libraries.” (Kasun M. Thambugala, Fungal Diversity, Vol. 57 (1), November, 2012)Table of ContentsMolecular basis of morphogenesis in fungi.- Tropic orientation responses of pathogenic fungi.- Hyphal fusion.- Signalling of infectious growth in Fusarium oxysporum.- Integrating Cdk signaling in Candida albicans environmental sensing networks.- Cell cycle and morphogenesis connections during the formation of the infective filament in Ustilago maydis.- Appressorium Function in Colletotrichum orbiculare and Prospect for Genome Based Analysis.- Morphogenesis in Candida albicans: How to stay focused.- Morphogenesis in Paracoccidioides brasiliensi.- Morphogenesis of Cryptococcus neoformans.- Morphogenesis and infection in Botrytis cinere.- Morphogenesis, growth and development of the grass symbiont Epichlöe festucae.- Cryptococcus-neutrophil interaction.-
£116.99
Springer Fachmedien Wiesbaden Botanisches Grundpraktikum
Book SynopsisDiese einmalige Kombination aus Lehrbuchtext und Praktikumsanleitung bietet sowohl einen theoretischen Hintergrund als auch eine praktische Anleitung, um die Histologie von Pflanzen kennenzulernen – ideal für Bachelor-Studierende. Ohne diese Grundlagen ist ein Verständnis von komplexen pflanzlichen Entwicklungsprozessen, die durch unterschiedliche Zelltypen und Gewebe mit verschiedenen Aufgaben geprägt sind, nicht möglich.In der 3. Auflage sind einige Objekte hinzugekommen, um das Spektrum des Praktikums zu erweitern. Verschiedene Präparate, die den Niederen Pflanzen zuzurechnen sind, wurden aufgenommen, um durch einfach gebaute Organismen das Verständnis für die Funktionsweise vielzelliger Pflanzen zu entwickeln. Das Kapitel Methoden haben die Autoren um die Bedienung des Lichtmikroskops, die Objektpräparation und Färbemethoden erweitert. Das Botanische Grundpraktikum ist vom Umfang her besonders für Bachelorstudierende geeignet.Table of ContentsDie Pflanzenzelle.- Die Sprossachse.- Das Blatt.- Die Wurzel.- Fortpflanzung und Entwicklung.- Methoden.- Lösungen zu den Aufgaben.- Glossar.- Literatur
£32.99
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Von der Zellenlehre zur Chromosomentheorie: Naturwissenschaftliche Erkenntnis und Theorienwechsel in der frühen Zell- und Vererbungsforschung
Table of Contents1 Wachstum naturwissenschaftlicher Erkenntnis: Einführung in die Wissenschaftstheorie von Thomas Kuhn.- 1.1 Paradigma, Anomalie, Krise und Revolution: Die Begriffe bei Thomas Kuhn.- 1.2 Kuhns Paradigma-Begriff aus der Sicht der Gestalttheorie.- 1.3 Bedeutung von Musterbeispielen in der Welterfahrung des heranwachsenden Kindes.- 1.4 Erster Exkurs: Unmögliche Gestalten.- 2 Wachstum naturwissenschaftlicher Erkenntnis: Von der Zelltheorie bis zur Chromosomentheorie der Vererbung.- 2.1 Zur Entwicklungsgeschichte der Mikroskopie und Histologie.- 2.2 Vorläufer der Zelltheorie: Franz Julius Ferdinand Meyen.- 2.3 Die Zelltheorie bei Schleiden und Schwann.- 2.4 Wissenschaftstheorie bei Schwann und Schleiden.- 2.5 Krise der Zelltheorie: Wie bilden sich Zellen?.- 2.6 Zellkern und Befruchtungslehre.- 2.7 Die Entdeckung der Chromosomen.- 2.8 Zweiter Exkurs: Zur Rolle von Wissenschaftssprache und esoterischen Objekten beim Wachstum wissenschaftlicher Erkenntnis.- 2.9 Kontinuität oder Auflösung der Chromosomen in der Interphase? Das Paradigma der Chromosomenindividualität.- 2.10 Chromosomentheorie der Vererbung vor 1900: August Weismanns Versuch einer „realen“ Theorie.- 2.11 Die Geburt der Genetik: Das Paradigma von Gregor Mendel.- 2.12 Chromosomentheorie der Vererbung nach 1900: Walter S. Sutton und Theodor Boveri.- 2.13 Cytogenetik in der Lyssenko-Ära: Ein illegitimer Paradigmawechsel.- 3 Betrachtungen zum Wachstum wissenschaftlicher Erkenntnis.- 3.1 Kuhns Theorie des Paradigmawechsels und die Geschichte der frühen Zell- und Verer-bungsforschung: Ein kritischer Vergleich.- 3.2 Wie wächst wissenschaftliche Erkenntnis?.- a) Was bedeutet „Wachstum“ im Erkenntnisprozeß?.- b) Gibt es Regeln für den Entwurf naturwissenschaftlicher Paradigmata? Anmerkungen zur Rolle von Theorie und Beobachtung.- 3.3 Der Wunsch nach geschlossenen Theorien und die Unvermeidbarkeit von Krisen im Wachstum der Erkenntnis. Bemerkungen aus dem Blickwinkel einer evolutionären Erkenntnistheorie.- 3.4 Sehen und räumliches Vorstellungsvermögen aus dem Blickwinkel einer evolutionären Erkenntnistheorie: der Mesokosmos als ,kognitive Nische‘ des Menschen.- Dritter Exkurs: Eschers Belvedere und das Raumproblem.- Vierter Exkurs: Naive und naturwissenschaftliche Raumerfahrung in einem zweidimensionalen Mesokosmos.- 3.5 Schlußbetrachtung: Entwicklung der Chromosomentheorie und Menschenbild.- 4 Anmerkungen.- 5 Postscriptum — Ludwik Fleck (1896–1961) der Vorläufer von Thomas Kuhn: Die Theorie vom Denkstil und den Denkstilumwandlungen in wissenschaftlichen Gemeinschaften.- 6 Literatur.- 7 Namenverzeichnis.- 8 Sachverzeichnis.
£61.74
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Cell Components
Book SynopsisModern Methods of Plant Analysis When the handbook Modern Methods of Plant Analysis was first introduced in 1954 the considerations were 1. the dependence of scientific progress in biology on the improvement of existing and the introduction of new methods; 2. the inavailability of many new analytical methods concealed in specialized journals not normally accessible to experimental plant biologists; 3. the fact that in the methods sections of papers the description of methods is frequently so compact, or even sometimes so incomplete, that experiments are difficult to reproduce. These considerations still stand today. The series was highly successful, seven volumes appearing between 1956 and 1964. Since today there is still a demand for the old series, the publisher has decided to resume publication of Modern Methods of Plant Analysis. It is hoped that the New Series will be as acceptable to those working in plant sciences and related fields as the early volumes undoubtedly were. It is difficult to single out the major reasons for success of any publication, but we believe that the methods published in the first series were up-to-date at the time and the descriptions as applied to plant material so complete in themselves that there was little need to consult other publications.Table of ContentsCell-Wall-Isolation, General Growth Aspects.- 1 Introduction.- 2 Isolation Procedures.- 2.1 Cell Breakage.- 2.2 Cell-Wall Recovery.- 2.3 Removal of Contaminants.- 3 Composition and Ultrastructure of Plant Cell Walls.- 3.1 Chemical Composition of Plant Cell Walls.- 3.1.1 Standard Extraction Procedures.- 3.1.2 Analysis of Polysaccharide Fractions.- 3.1.2.1 Chemical Methods.- 3.1.2.2 Physical Methods.- 3.2 Supramolecular Organization of Plant Cell Walls.- 3.2.1 Morphological Observations.- 3.2.2 Selective Staining of Polysaccharides.- 3.2.2.1 Visualization of Esterified Carboxyl Groups.- 3.2.2.2 Detection of Acidic Functions.- 3.2.2.3 Periodic Oxidation of Glycol Groups.- 3.2.3 Visualization of Lignin.- 3.2.4 Identification of Wall Components by Means of Affinity Methods.- 3.2.5 Detection and Estimation of Cations.- 3.2.6 Ultracryotomy.- 4 Properties of Plant Cell Walls.- 4.1 Exchange Properties of Plant Cell Walls.- 4.2 Enzymatic Properties.- 4.2.1 Cytochemical Investigations.- 4.2.1.1 Cell-Wall Phosphatase Activities.- 4.2.1.2 Cell-Wall Peroxidase Activities.- 4.2.2 Biochemical Investigations.- 4.2.2.1 Properties of Immobilized and Solubilized Cell-Wall Enzymes.- 4.2.2.2 Biological Functions.- 4.3 Mechanical Properties.- 5 Growth Aspects.- 5.1 Cell-Wall Loosening.- 5.1.1 Wall-Loosening-Inducing Agents.- 5.1.2 Nature of the Broken Bonds.- 5.2 Deposition of Wall Material.- 5.3 Growth Direction.- References.- Cell-Wall Chemistry, Structure and Components.- 1 Introduction.- 2 Histochemical Analysis of Cell Walls.- 2.1 Specific Stainings.- 2.2 Staining with Fluorescent Brightener.- 2.3 Anisotropy Test.- 2.4 Selective Dissolution.- 2.4.1 Alkali Treatment.- 2.4.2 Cuprammonium Solution (Schweitzer’s Reagent) Treatment.- 2.4.3 Enzymatic Digestion.- 3 Quantitative Analysis of Cell Walls.- 3.1 Plant Materials.- 3.1.1 Pure Culture.- 3.1.2 Synchronous Culture.- 3.1.3 Harvesting of Cells.- 3.2 Measurement of Cell Growth.- 3.3 Preparation and Fractionation of Cell Walls.- 3.3.1 Disruption of Cells.- 3.3.2 Separation and Purification of Cell Walls.- 3.3.3 Fractionation of Cell Walls.- 3.4 Quantitative Analysis of Whole Cell Walls.- 3.4.1 Gravimetry.- 3.4.2 Turbidimetry.- 3.4.3 Colorimetry.- 4 Qualitative Analysis of Cell-Wall Materials.- 4.1 Acid Hydrolysis.- 4.2 Enzymatic Hydrolysis.- 5 Chromatographic Analysis of Cell-Wall Constituents.- 5.1 Thin-Layer Chromatography.- 5.1.1 Neutral Sugars and Uronic Acids.- 5.1.2 Amino Acids and Amino Sugars.- 5.1.3 Thin-Layer Chromatographic Analyses of the Constituents of Chlorella Cell Walls.- 5.2 Liquid Chromatography.- 5.2.1 Amino Acids and Amino Sugars.- 5.2.2 Neutral Sugars.- References.- Protoplasts—for Compartmentation Studies.- 1 Introduction.- 2 Advantages of the Use of Protoplasts for Compartmentation Studies.- 3 Protoplast Isolation and Its Effect on Cellular Metabolism.- 3.1 Isolation Procedures.- 3.2 Effect of Isolation pH.- 3.3 Effect of Plasmolysis.- 3.4 Effect of Enzyme Contaminants.- 4 Protoplast Lysis.- 5 Protoplast Fractionation.- 5.1 Density Gradient Fractionation.- 5.2 Rapid Fractionation Procedures.- 6 Methods to Relate Protoplast Activity to That of Intact Tissue.- 7 Concluding Remarks.- References.- The Marker Concept in Cell Fractionation.- 1 Introduction.- 2 The Marker Concept.- 2.1 Basic Concepts.- 2.2 Types of Marker.- 2.2.1 Morphological.- 2.2.2 Cytochemical.- 2.2.3 Biochemical.- 3 Preservation of Marker Enzyme Activity During Cell Disruption.- 3.1 Choice of Material.- 3.2 Homogenization Procedure.- 3.3 Use of Additives in the Homogenization Medium.- 3.4 Gel Filtration to Remove Soluble Hydrolytic Activity.- 4 Methods Used to Separate Markers.- 4.1 General Approaches to Cell Fractionation.- 4.2 Differential Centrifugation.- 4.2.1 Preparative vs. Analytical Cell Fractionation.- 4.2.2 Need for Quantitation.- 4.2.3 Problems with Complete Quantitation and Interpretation of Data.- 4.3 Linear Density Gradient Centrifugation.- 4.3.1 Density Gradient Material.- 4.3.2 Pelleted vs. Unpelleted Overlays.- 4.3.3 Soluble Enzyme Contamination in Gradients.- 4.3.4 Equilibrium Density Centrifugation (Isopycnic Conditions).- 4.3.5 Other Factors Which Influence Marker Enzyme Profiles Across a Gradient.- 4.3.6 Need for Quantitation and Lack of Negative Marker Activity.- 5 Concluding Remarks.- References.- Plasma Membranes.- 1 Introduction.- 2 Theory of Phase Partition.- 2.1 The Phase System.- 2.2 Partitition of Membrane Particles.- 2.3 Effects of Polymer Concentrations.- 2.4 Effects of Salts.- 2.5 Multistep Procedures.- 3 Experimentals.- 3.1 Chemicals.- 3.2 Preparation Procedure.- 4 Purity of the Preparations.- 4.1 Specific Staining.- 4.2 K+-Stimulated, Mg2+-Dependent ATPase.- 4.3 Glucan Synthetase II.- 4.4 Light-Reducible b-Cytochrome.- 4.5 Markers for Contaminants.- 5 Protein and Lipid Composition.- 6 Surface Properties of the Isolated Vesicles.- References.- Vacuoles.- 1 Introduction.- 2 Methods of Isolation.- 2.1 Isolation of Vacuoles from Meristematic Tissues.- 2.2 Isolation of Vacuoles from Mature Plant Tissue.- 2.2.1 Isolation of Mature Vacuoles from Protoplasts—Methods pre 1981.- 2.2.2 Isolation of Mature Vacuoles from Protoplasts—Methods post 1981.- 2.2.3 Isolation of Mature Vacuoles Directly from Tissue—Methods pre 1981.- 2.2.4 Isolation of Mature Vacuoles Directly from Tissue—Methods post 1981.- 2.2.5 Preparation of Lutoids from Hevea Latex.- 2.2.6 Comments on Methods for Isolating Vacuoles from Higher Plants.- 2.2.7 Isolation of Proton-Pumping Vesicles.- 2.2.8 Preparation of Vacuoles from Yeast Neurospora.- 3 Isolation of Tonoplast and Tonoplast Markers.- 4 Comments on Physiological Functions.- 5 Concluding Remarks.- References.- Protein Bodies.- 1 Introduction.- 2 Special Consideration in Isolation of Protein Bodies.- 3 Nonaqueous Preparation in Glycerol.- 4 Nonaqueous Preparation in Hexane and Carbon Tetrachloride.- 5 Aqueous Preparation in Sources Gradients.- 6 Subfractionation of Isolated Protein Bodies.- 7 Analyses.- References.- Lipid Bodies.- 1 Introduction.- 2 Ontogeny.- 3 Isolation.- 4 Markers of Lipid Bodies.- 5 Assays.- 5.1 Fluorometric Assay.- 5.2 Colorimetrie Assay.- References.- Chloroplasts as a Whole.- 1 Introduction.- 2 Considerations of Integrity and Purity.- 3 Chloroplasts from Protoplasts.- 4 The Use of Silica Sols in Density Gradient Purification of Chloroplasts.- 5 General Notes on Isolation Procedures.- 6 Specific Isolation Protocols.- 6.1 Higher Plants.- 6.1.1 C3 Plants.- 6.1.2 C4 Plants.- 6.1.3 CAM Plants.- 6.2 Algae.- 6.2.1 Volvocales.- 6.2.2 (Ceramiaceae, Rhodophyta)—Griffithsiamonilis.- 6.2.3 Siphonales.- 6.2.4 (Xanthophyceae) Bumilleriopsisfiliformis.- 6.2.5 (Euglenophyceae) Euglenagracilis.- 7 Additional Comments on Chloroplast Isolation.- 8 Abbreviations.- References.- Purification of Inner and Outer Chloroplast Envelope Membranes.- 1 Introduction.- 2 General Considerations.- 3 The Procedure.- 3.1 Reagents and Equipment.- 3.1.1 Solutions.- 3.1.2 Materials.- 3.2 Growth of Peas and Purification of Intact Chloroplasts.- 3.3 Purification of Inner and Outer Envelope Membranes.- 4 Properties of the Isolated Membranes.- 4.1 Purity.- 4.1.1 Cross-Contamination by Envelope Membranes.- 4.1.2 Contamination by Thylakoids.- 4.1.3 Contamination by Stroma.- 4.2 Other Properties.- 5 Modifications of the Procedure.- 5.1 Alternate Methods of Chloroplast Rupture.- 5.2 Purification Subsequent to Rupture.- 5.3 Application to Other Tissues.- 6 Other Procedures.- References.- The Major Protein of Chloroplast Stroma, Ribulosebisphosphate Carboxylase.- 1 Introduction.- 2 Characteristics of RuBP Carboxylase.- 2.1 Molecular Arrangement and Physical Structure of Subunits.- 2.2 Molecular Structure.- 2.3 Biosynthesis and Assembly of Subunits.- 2.3.1 Large Subunit.- 2.3.2 Small Subunit.- 2.3.3 Subunit Heterogeneity.- 2.3.4 Coordinate Control of Subunit Synthesis.- 2.4 Catalytic Mechanism.- 2.4.1 Activation and Role of Mg2 +.- 2.4.2 Carboxylation of RuBP.- 2.4.3 Oxygenation of RuBP.- 2.4.4 Localization of Catalytic and Activator Site.- 3 Practical Aspects.- 3.1 Purification.- 3.1.1 Summary of Techniques.- 3.1.2 Interfering Compounds.- 3.1.3 Choice of Extraction Buffer and Grinding Procedures.- 3.1.4 Protein Determination.- 3.1.5 Example: RuBP Carboxylase from Soybean Leaves.- 3.2 Assay.- 3.2.1 Substrates.- 3.2.2 Activation of RuBP Carboxylase.- 3.2.3 Continuous Spectrophotometry Assay for RuBP Carboxylase Activity.- 3.2.4 Discontinuous Assays for RuBP Carboxylase Activity.- 3.2.4.1 Radiochemical Assay with [14C]NaHCO3.- 3.2.4.2 Radiochemical Assay Using [14C]NaHCO3 and [l-3H]RuBP.- 3.2.4.3 Discontinuous Assay Using Nonlabeled Substrates.- 3.2.5 Assays for RuBP Oxygenase.- 3.2.6 Kinetic Parameters of RuBP Carboxylase and RuBP Oxygenase.- 4 Conclusion.- References.- The Chloroplast Thylakoid Membrane—Isolation, Subfractionation and Purification of Its Supramolecular Complexes.- 1 Introduction.- 2 Function and Organization of the Thylakoid Membrane.- 3 Isolation of Thylakoid Membranes.- 4 Thylakoid Membrane Subfractionation.- 4.1 Photosystem I Stroma Lamellae Thylakoids.- 4.2 Photosystem II Oxygen Evolving Thylakoid Preparations.- 4.2.1 Isolation by Press Treatment and Phase Partition.- 4.2.2 Isolation by Detergent Fractionation.- 4.2.3 Choice of Preparation.- 4.3 Separation of Inside-Out and Right-Side-Out Thylakoid Vesicles with the Same Composition.- 5 Isolation of Thylakoid Supramolecular Complexes.- 5.1 The Photosystem I Complex and the Light-Harvesting Complex of Photosystem II (LHC II).- 5.2 The Light-Harvesting Complex of Photosystem I (LHC I).- 5.3 The Inner Core Complex of Photosystem II (CC II).- 5.4 The Cytochrome b/f Complex.- 5.5 The ATP Synthase (CF0-CF1).- References.- The Isolation and Characterization of Nongreen Plastids.- 1 Introduction.- 2 The Terminology of Nongreen Plastids.- 2.1 Proplastids.- 2.2 Etioplasts.- 2.3 Chromoplasts.- 2.4 Amyloplasts.- 2.5 Leucoplasts.- 2.6 Other Nongreen Plastids.- 3 Basics of Plastid Isolation and Separation.- 3.1 Experimental Design.- 3.2 Isolation Medium.- 3.3 Tissue Disruption.- 3.4 General Methods of Chloroplast Isolation.- 4 Isolation of Nongreen Plastids from Developing Ricinus Endosperm.- 4.1 Rate-Zonal Sedimentation.- 4.1.1 Protocol.- 4.1.2 Analysis.- 4.1.3 Comments.- 4.2 Isopycnic Banding on Linear Sucrose Gradients.- 4.2.1 Protocol.- 4.2.2 Comments.- 4.3 Rate-Zonal Sedimentation on Linear Sucrose-Magnesium Co-Gradients.- 4.3.1 Protocol.- 4.3.2 Comments.- 4.4 Rate-Zonal Sedimentation on Discontinuous Sucrose Gradients.- 4.4.1 Protocol.- 4.4.2 Comments.- 4.5 Rate-Zonal Sedimentation on Discontinuous Percoll Gradients.- 4.5.1 Protocol.- 4.5.2 Comments.- 4.6 Nonaqueous Methods.- 4.6.1 Isopycnic Banding on Linear Hexane-CCl Gradients.- 4.6.2 Silicon Oil Centrifuged Filtration.- 4.7 Noncentrifugal Methods.- 4.7.1 Gel Permeation.- 4.7.1.1 Materials.- 4.7.1.2 Protocol.- 4.7.1.3 Comments.- 4.7.2 Phase Partition.- 4.7.3 Unit-Gravity Sedimentation.- 5 Metabolic Capabilities of Ricinus Endosperm Plastids.- 5.1 Glycolysis, the Pentose-Phosphate Pathway and Fatty Acid Synthesis.- 5.2 The Calvin Cycle.- 5.3 Nitrogen Metabolism.- 5.4 Terpenoid Metabolism.- 6 Composition and Biochemical Properties.- 6.1 Structure.- 6.2 Protein Composition.- 6.3 Membranes.- 6.4 Nucleic Acids.- 7 Future Prospects.- References.- Mitochondria.- 1 Introduction.- 2 Preparation for DNA Analysis.- 2.1 Cytoplasmic Male Sterility and Structure ot Mitochondrial DNA.- 2.2 Isolation of Mitochondria for DNA Preparation.- 2.3 Preparation of Mitochondrial DNA.- 2.4 Electrophoresis of Mitochondrial DNA.- 2.5 Restriction Analysis of Mitochondrial DNA.- 2.6 Notes on Mitochondrial DNA Studies.- 3 Preparation of Intact Mitochondria for Oxidative Studies.- 3.1 Introduction.- 3.2 Mitochondrial Preparation and Purification.- 3.3 Tests for Integrity of Mitochondria.- 3.3.1 Succinate: Cytochrome c Reductase.- 3.4 Tests for Integrity.- 3.4.1 Measurement for Oxygen Consumption for Respiratory Control and P/O Ratios.- 3.5 Notes on the Methods.- References.- Endoplasmic Reticulum.- 1 Introduction.- 2 Structure and Organization of the ER.- 3 Interactions Between Tubular and Cisternal ER.- 3.1 Role in Protein Transport.- 3.2 Role in Cell Division.- 4 Synthesis and Degradation of ER.- 4.1 Membrane Proteins.- 4.2 Membrane Lipids.- 5 Isolation and Characterization of ER.- 5.1 Isolation Media.- 5.2 Tissue Homogenization.- 5.3 Organelle Isolation.- 5.3.1 Molecular Sieve Chromatography.- 5.3.2 Differential Centrifugation.- 5.3.3 Density Gradient Centrifugation.- 5.4 Identification of ER Membranes.- 5.4.1 Magnesium Shift.- 5.4.2 Marker Enzymes.- 5.4.3 Auxin Binding.- 5.4.4 Calcium Transport.- 5.4.5 Structural Proteins.- 5.5 Concluding Remarks.- References.- Polyribosomes.- 1 Introduction.- 2 Isolation of Polysomes from Plant Cells.- 2.1 Factors that Affect the Stability and Recovery of Polyribosomes.- 2.2 Tissue Preparation.- 2.3 Subcellular Fractionation and Polysome Isolation.- 3 Purification and Analysis of Polyribosomes.- 3.1 Sucrose Gradient Centrifugation.- 3.2 Purification of Polysomes with Discontinuous Sucrose Gradients.- 3.3 Analysis of Polyribosome Profiles.- 4 Polyribosome Extraction Buffers.- 4.1 pH.- 4.2 Potassium Chloride.- 4.3 Magnesium Chloride.- 4.4 Reducing Agents.- 4.5 Chelation of Divalent Metals.- 4.6 Proteinase K.- 4.7 Other Ribonuclease Inhibitors.- 5 Uses of Purified Polyribosomes.- 5.1 Changes in Protein Synthetic Activity.- 5.2 In Vitro Protein Synthesis.- 5.3 Purification of mRNA’s.- 5.4 Subcellular Distribution of mRNA’s.- References.- The Nucleus—Cytological Methods and Isolation for Biochemical Studies.- 1 Introduction.- 2 Structure of the Plant Nucleus and Implications for Nuclear Isolation and Staining.- 3 Cytology.- 3.1 Nuclei of Whole Cells.- 3.1.1 Feulgen Microspectrophotometric Methods.- 3.1.2 Microfluorometry DNA Determination.- 3.2 Staining Nuclei During Isolation.- 3.3 Nucleolus Staining.- 3.4 Chromosome Staining.- 3.5 Other Nuclear Stains.- 4 Isolation of Plant Nuclei—General.- 4.1 Isolation of Plant Nuclei—Methods.- 4.1.1 Nuclei from Tobacco Callus Cultures for RNA Synthesis Studies.- 4.1.2 Nuclei from Tobacco Cells in Culture—for General Purpose Studies.- 4.1.3 Nuclei from Soybean Cells—for DNA Studies.- 4.1.4 Plant-Root Nuclei—for DNA Analysis.- 5 Summary.- References.- Microtubules.- 1 Introduction.- 2 Extraction of Microtubule Proteins.- 3 Purification of Tubulin and MAP’s.- 3.1 DEAE-Sephadex Ion Exchange Chromatography.- 3.2 Phosphocellulose Chromatography.- 3.3 Affinity Chromatography.- 3.4 Cycles of Polymerisation and Depolymerisation.- 3.4.1 Pre-Conditions for Microtubule Assembly.- 3.4.2 Microtubule Assembly in the Presence or Absence of Glycerol.- 3.4.3 The Dynamics of Polymerisation and the Use of Taxol.- 3.4.4 Co-Polymerisation.- 4 Fractionation and Identification of Tubulin by SDS-PAGE.- 5 Colchicine-Binding Assay for Tubulin.- 6 Immunochemical Methods of Analysis.- 6.1 Radioimmunoassay.- 6.2 Enzyme-Linked Immunosorbent Assay (ELISA).- 6.3 Antibody Purification of Antigen-Affinity Column.- 6.4 Western Blots.- 7 Concluding Remarks.- References.
£85.49
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Intracellular Antibodies: Development and Applications
Book SynopsisRecent advances in the field of recombinant antibodies have permitted the manipulation of genes encoding specific antibodies, thus allowing their ectopic expression in a wide variety of non-lymphoid cells. This volume describes how the ectopic expression of antibodies, as secreted or as intracellularly retargeted molecules, can be exploited to block biological functions or to confer new phenotypic traits (e.g. resistance to a virus). This is the first book describing this emerging technology, which is receiving increasing attention for application in many different fields and biological systems - from human gene therapy to plant biotechnology.Table of Contents1. Intracellular and Intercellular Immunization.- 2. Current Methods for Genotypic and Phenotypic Knock-Outs in Mammalian Cells.- 3. Recombinant Antibodies for Ectopic Expression.- 4. Assembly and Folding of Antibodies in Natural and Artificial Environments.- 5. Protein Sequence Motifs Involved in Intracellular Trafficking.- 6. Intercellular Immunization.- 7. Intracellular Immunization.- 8. Gene Therapy and Research Applications of Intrabodies for Human Infectious Diseases.- 9. Plantibodies: Immunomodulation and Immunotherapeutic Potential.- 10. From Phage Libraries to Intracellular Immunization.- 11. Perspectives and Conclusions.
£42.74
Springer Fachmedien Wiesbaden PCR - Polymerase-Kettenreaktion
Book SynopsisZiel dieses handlichen, deutschsprachigen Laborhandbuches ist es, die Standard- und Spezialanwendungen der PCR in praxisnaher und verständlicher Form darzustellen. Es wendet sich an Diplomanden, Doktoranden, Wissenschaftler und TAs, die die Möglichkeiten der PCR für ihre molekularbiologischen oder diagnostischen Fragestellungen nutzen möchten.In vorliegender 2. Auflage wurden die etablierten Methoden aktualisiert und wichtige neue Applikationen (z.B. Next Generation Sequencing oder die Emulsions-PCR) hinzugefügt. Da die Automation bei den molekularbiologischen Applikationen fortschreitet, erfordern gerade die letztgenannten Methoden immer weniger Handarbeit, wobei das generelle Verständnis über die einzelnen Schritte vorhanden sein muss. Dieses ‚Know-How‘ erhalten Sie im vorliegenden PCR-Methodenbuch.Table of ContentsEinleitung.- Allgemeine PCR-Parameter.- PCR als Detektionsmethode.- PCR als Klonierungsmethode.- PCR für die Standard-Klonierung.- T/A-Cloning.- Ligase-unabhängige-Klonierung (LIC).- UNG-Klonierung.- Surf-Klonierung.- Megaprime-PCR.- RT-PCR.- RACE-PCR.- Quantitative PCR.- Real-Time-PCR.- Colony-PCR.- PCR zur Mutationsanalyse.- Nested-PCR.- DOP-PCR.- Alu- (IRS) PCR.- PCR-Optimierung.- 1-Sekunden-PCR.- Long Distance-PCR.- Genomtypisierung mit der PCR.- Differential Display PCR Firmenverzeichnis Internet-Adressen rund um die PCR Sachregister.
£52.24
Springer-Verlag Berlin and Heidelberg GmbH & Co. KG Signal Transduction: Pathways, Mechanisms and
Book SynopsisTable of ContentsG Proteins and G Protein–Coupled Receptors.- Traditional GPCR Pharmacology and Beyond.- Regulation of Intraneuronal Trafficking of G-Protein-Coupled Receptors by Neurotransmitters In Vivo.- Small GTPases and Their Role in Regulating G Protein-Coupled Receptor Signal Transduction.- Regulation of G Protein Receptor Coupling, Mood Disorders and Mechanism of Action of Antidepressants.- Dysregulation of G Protein-Coupled Receptor Signaling in Cancer.- Growth Factors.- Insulin Signaling in Normal and Diabetic Conditions.- Epidermal Growth Factor (EGF) Receptor Signaling and Cancer.- Leptin Signaling Pathway.- Signaling in Normal and Pathological Angiogenesis.- Signaling Platforms.- Spatial and Temporal Control of Cell Signaling by A-Kinase Anchoring Proteins.- Mitochondria, a Platform for Diverse Signaling Pathways.- Mitogen-Activated Protein Kinases and Their Scaffolding Proteins.- Molecular and Functional Determinants of Ca2+ Signaling Microdomains.- Nuclear Receptors / Transcription.- Eukaryotic Gene Transcription.- Estrogen Signaling Mechanisms.- Signal Transduction Pathways Involved in Glucocorticoid Actions.- Reactive Signaling Molecules.- Cellular Signaling by Reactive Oxygen Species: Biochemical Basis and Physiological Scope.- Soluble Guanylyl Cyclase: The Nitric Oxide Receptor.- Cell Cycle, Cell Death and Cancer.- Distinct Roles of the Pocket Proteins in the Control of Cell Cycle.- Activation of the p53 Tumor Suppressor and its Multiple Roles in Cell Cycle and Apoptosis.- Aging and Cancer: Caretakers and Gatekeepers.- Signal Transduction in Embryonic Stem Cells and the Rise of iPS Cells.- Erratum to.
£170.99
Harwood-Academic Publishers Programmed Cell Death
Book SynopsisAlthough general morphological features have been used to consistently identify the changes in cell ultrastructure occurring during apoptosis, as distinct from necrosis, important advances have been achieved more recently in the investigation of the cellular and molecular aspects of this process. This book brings together the latest international research on the complex subject of programmed cell death, and covers such areas as the biochemical mechanisms, introduction of DNA fragmentation, enetic regulation, and the importance of apoptosis in the immune system, particularly during T-cell development, and in cancer. The comparison of a number of common signal transduction pathways with those involved in cell growth highlights an important relationship between apoptosis and the control of cell proliferation.Table of ContentsBiochemical Mechanisms; DNA Fragmentation; Genetic Regulation; Apoptosis in the Immune System; Apoptosis in Cancer.
£209.00
Taylor & Francis Ltd Estrogen and the Vessel Wall
Book SynopsisEstrogen and the Vessel Wall marks the start of a new era in understanding the cardiovascular actions of estrogen. It examines the hypothesis, derived from a growing body of epidemiological data, that estrogen acts directly upon the vessel wall to produce beneficial effects in the cardiovascular system.Inspired by a satellite symposium of the IX International Vascular Biology Meeting, additional topics have been included, covered by leading experts. All subjects have been brought together here to produce an outstanding and integral publication, a milestone in the field of Vascular Research.Table of ContentsPart I - Regulation of Gene Expression by the Estrogen Receptor Part II - Vascular Endothelium 1. Role of Estrogen in Endothelial Cell Behaviour 2. Modulation of Cellular Adhesion Molecules and Endothelial Nitric Oxide Synthase by Estrogen 3. Non-Genomic Actions of Estrogens: Estrogens as Antioxidants Lipoprotein Metabolism in the Vessel Wall 4. Estrogen and Vasomotor Tone 5. Endothelium-Dependent Vasoconstrictors Part III - Lipoproteins, Hemostasis and Leukocytes 1. Estradiol and Accelerated Transplant Atherosclerosis 2. Statistical Analysis of Ionic Channel Current Fluctuations 3. Natural Variability in the Geometry of Dendritic Branching Patterns 4. The Multiple Equivalent Cylinder Model 5. Parameter Estimation Algorithms for the Shunt Cable Model 6. Determination of Cable Parameters for Neurons with Gap Junctions 6. Ionic Current Modeling of Neurons in the Outer Retina 7. Ephatic Interactions Between Neurons Part IV - Regulation of Vasomotor Function 1. Estrogens and Coronary Artery Reactivity in 2. Estrogen Receptor in the Cardiovascular System Part V - Atherosclerosis and Vascular Diseases 1. Atherosclerotic Monkeys 2. Cloning Expression and Function of a Novel Estrogen Receptor
£166.25
Gordon and Breach Cytokines and Cytokine Receptors: Physiology and
Book SynopsisThe field of cytokine research is expanding at a rapid pace Contributions from the major leading groups in the world on the structure and biological properties of cytokine and cytokine receptors, as well as integrated reviews on cytokines in various physiological and pathological conditions were presented in three issues of International Reviews of Immunology This collection of articles provided a unique source of information However, important discoveries are emerging very rapidly and some of the reviews written in 1997 are already outdated In this book, the editors assemble reviews that have been updated by their authors to include all the recent publications and unpublished data from the authors' laboratories This volume should serve as an excellent reference source for all those concerned by the multiple faces of cytokines in basic research and in the clinic
£200.00
Gordon and Breach Glycans in Cell Interaction and Recognition
Book SynopsisGlycoproteins play an important role in the regulation of gene expression, cell growth, migration, differentiation and apoptosis. Over the last decade, research has highlighted the therapeutic implications of glycoproteins for many physiological and pathological processes, such as inflammation, arthritis and metastasis. The first part of the book deals with the structure and biosynthesis of glycoconjugates; the second part addresses the pathological roles played by glycoconjugates and their potential therapeutic applications.
£125.00
Taylor & Francis Ltd Cell Adhesion and Migration in Skin Disease
Book SynopsisThe past few years have seen considerable advances in our understanding of the molecular basis underlying cutaneous cell adhesion mechanisms. Co-authored by a number of leading experts in the field ^Cell Adhesion and Migration in Skin Disease provides a comprehensive overview of the critical role played by cell adhesion in determining the structure and function of both healthy and diseased human skin. The book is divided into three main sections, with each one addressing a principal function of adhesion molecules.The first part focuses on the epidermis, which as the skin's outermost layer, acts as the human body's primary barrier of defence. Roles played by cytoskeletal intermediate filaments and junctional complexes in cutaneous cell adhesion are emphasised with descriptions of blistering skin diseases that can arise if these molecules malfunction.The second part describes the macromolecular interactions responsible for the anchorage of cells to the underlying extracellular basement membrane. The experimental approaches detailed in the text not only reveal how the molecular components of the dermal-epidermal junction have been elucidated, but also highlight how mutations in the genes which encode these molecules are responsible for many heritable skin diseases. Leukocytes continually infiltrate the skin and patrol it for potentially harmful pathogens. Control of leukocyte adhesion to resident cells within the skin and to the extracellular matrix plays a key role in controlling these processes. These mechanisms constitute the primary focus of the final section. The pivotal role of leukocytes is examined in conjunction with the chronic inflammatory diseases which arise when components of the skin's finely tuned defence strategy go awry and the potential for these anomalies to be pinpointed as important immunotherapeutic targets for skin diseases.Table of Contents1. Cell-Cell Attachment 2. The Cornified Cell Envelope 3. Keratins and Keratin Disorders 4. Desmosomes 5. Cell-Matrix Attachment 6. Protein-Protein Interactions at the Dermal-Epidermal BMZ 7. Biology and Pathology of Hemidesmosomes 8. Dermal-Epidermal Adhesion 9. Leukocyte Trafficking in Skin Diseases 10. Skin Homing Lymphocytes 11. T-cell Accessory Molecules 12. Animal Models of Skin Inflammation 13. Langerhans Cell Migration 14. Leukocyte Adhesion and Accessory Molecules as Therapeutic Targets for Inflammatory Skin Diseases
£190.00
Brill Cells, Proteins and Materials: Festschrift in
Book SynopsisProteins, Cells and Materials contains a collection of articles, which constitute together the complete Festschrift in honor of the 65th birthday of Dr. John L. Brash. For the first time these articles - published previously in several special issues of the Journal of Biomaterials Science Polymer Edition - have been compiled into one comprehensive volume. Over the past 40 years John Brash, a member of the Editorial Board of the Journal of Biomaterials Science Polymer Edition, has distinguished himself in the field of biomaterials. Much of his efforts have focused on detailed studies of blood–surface interactions, particularly those of plasma proteins. His multi-faceted approach recognises the importance of hemodynamics, transport and surface phenomena in the gross effects that result from blood–surface contact. In this book articles on the most recent development in these areas are collected and will thus provide a wealth of information of current research to specialists in the above-mentioned fields.Table of ContentsForeword, Introduction, Letter, I. Proteins, II. Cells, III. Materials
£218.50
Pan Stanford Publishing Pte Ltd Quantitative Proteome Analysis: Methods and
Book SynopsisThis book focuses on the advantages and disadvantages of each of the commonly used quantitative proteomic methods in terms of accuracy, sensitivity, and reproducibility. It also concentrates on the effective applications of these methods that resulted in many discoveries of the role of the proteins expressed in living cells and biological fluids. The book focuses on the description of advantages and disadvantages of each of the commonly used quantitative proteomic methods, and provides concise descriptions of the effective applications of these methods.Table of ContentsFD-LC-MS/MS. 2-D DIGE. Mass Spectrometry Utilizing ICAT. Proteomic Analyses of Post-translational Modifications. Cardiovascular Proteomic Analysis. Proteome in Neurodegenerative Diseases. Liver Disease-Related Proteome. Respiratory Disease Related Proteome. Renal Disease-Related Proteome. Aging-Related Proteome. Phosphoproteomics of Tumor Cell Lines. HCV Infection and Mitochondria Proteomics. Infectious Disease by SELDI. Quantitative Proteomic Analysis of HIV Infection.
£147.25
Pan Stanford Publishing Pte Ltd NanoCellBiology: Multimodal Imaging in Biology
Book SynopsisThis book provides a comprehensive understanding of the discovery of a new cellular structure the "porosome," which is the universal secretory machinery in cells; the protein assembly, biomineralization, and biomolecular interactions; the molecular evolution of protein structure; the use of magnetic nanoparticles for transformative application in medicine and therapy, and the new and novel imaging approach of electrical impedance spectroscopy in biology. It be used for college courses in nanomedicine, nano cell biology, advanced nanotechnology, and biotechnology at the undergraduate and graduate level.Trade Review"NanoCellBiology: Multimodal Imaging in Biology and Medicine, edited by Bhanu P. Jena and Douglas J. Taatjes, is a collection of chapters that describe examples of the use of AFM, electron microscopy, photon correlation spectroscopy, confocal microscopy, fluorescence/CD spectroscopy, and other imaging approaches for revealing important structures and their function in cells. A wonderful example is the subject of the first several chapters, which describe the discovery of the porosome. Discovered in the 1990s, first in pancreatic acinar cells, the porosome is now considered a universal secretory portal in cells. The remaining chapters add to this excellent collection of studies employing high-resolution imaging to examine, for example, amylin aggregation, mRNA nanomachines, DNA delivery nanosystems, and other interesting applications of nanocellbiology."Prof. James A. Spudich, Stanford University School of Medicine, USA"Bhanu P. Jena is a pioneer nanocell biologist, whose seminal discovery of a new cell structure called the ‘porosome,’ has provided a molecular understanding of the fractional release of intravesicular contents from cells during secretion. In this book, co-edited by Jena and Douglas J. Taatjes, experts in the field present examples of powerful imaging modalities that have been extremely valuable in elucidating a wide range of normal cellular events, as well as in studying disease progression, detection, and treatment. Chapters in the book provide a lucid explanation of the subject matter, with ample illustrations presented for clarity. This is a timely book, filled with useful resources—a must-read for both researchers and students in cell biology, physiology, biophysics, nanobiology, and nanomedicine."Prof. Walter F. Boron, Case Western Reserve University, USA"NanoCellBiology: Multimodal Imaging in Biology and Medicine is a delightful book co-edited by a pioneer in the subject, Bhanu P. Jena, whose discovery of a new cellular structure, the porosome, in 1996 resulted in a paradigm shift in our understanding of cell secretion and revolutionized our understanding of the unit of life—the cell. This book, edited by Bhanu P. Jena and Douglas J. Taatjes, introduces to the reader a number of exciting subjects within the field of nanoscience and nanomedicine, and the various tools and approaches to solve them for the benefit of science and humanity. I thoroughly enjoyed reading the book and highly recommend to both students in the pure sciences and medicine."Prof. Lloyd L. Anderson, Iowa State University, USATable of ContentsPorosomes -The Universal Secretory Portals In Cells: A Brief Essay. The Hair Cell Porosome: Molecular and Synaptic Implications. The Neuronal Porosome Complex in Mammalian Brain: A Study Using Electron Microscopy. Granule Size Distribution Suggests Mechanism: The Case for Granule Growth and Elimination as a Fusion Nano-Machine. Probing Protein Assembly, Biomineralization, and Biomolecular Interactions by Atomic Force Microscopy. High-Resolution Imaging of Amylin Aggregation and Internalization in Pancreatic Cells: Implications in Health and Diseaser. Repair of Nanodefects in a 2-Dimensional Crystal Anticoagulant Shield in the Antiphospholipid Syndrome: Novel Molecular Strategies Assessed by Atomic Force Microscopy. A novel approach to study molecular evolution: Detection of ancestral conformation hidden in present-day proteins using antibody as nanostructure probes. mRNA Nanomachines and Stress Reprogramming Following Brain Ischemia. Physical Properties and Biomedical Applications of Superparamagnetic Iron Oxide Nanoparticles. Atomic Force Microscopy Imaging of DNA Delivery Nano-systems. Impedance Spectroscopy for Characterization of Biological Functions. Index.
£109.25
Pan Stanford Publishing Pte Ltd Handbook of H+-ATPases
Book SynopsisThis handbook on vacuolar and plasma membrane H+-ATPases is the first to focus on an essential link between vacuolar H+-ATPase and the glycolysis metabolic pathway to understand the mechanism of diabetes and the metabolism of cancer cells. It presents recent findings on the structure and function of vacuolar H+-ATPase in glucose promoting assembly and signaling, in addition to describing the regulatory mechanisms of vacuolar H+-ATPase in yeast cells, neural stem cells, kidney cells, cancer cells, as well as under diabetic conditions. Table of ContentsVacuolar H+-ATPase Assembly. Structure of Prokaryotic V type ATPase/synthase. The function of V-ATPase in the degradation of gluconeogenic enzymes in the yeast vacuole. The Role of Vacuolar ATPase in the Regulation of Npt2a Trafficking. Cytosolic pH regulated by glucose promotes V-ATPase assembly. Vacuolar H+-ATPase (V-ATPase) activated by glucose, a possible link to diabetic disease. Vacuolar proton pump (V-ATPase) and insulin secretion. Role of V-ATPase, cytohesin-2/Arf6 and aldolase in regulation of endocytosis: Implications for diabetic nephropathy. Renal Vacuolar H+-ATPase Regulation. Long-term Regulation of Vacuolar H+-ATPase by Angiotensin II in Proximal Tubule Cells. Vacuolar H+-ATPase in Distal Renal Tubular Acidosis and Diabetes. Vacuolar H+-ATPase in Cancer and Diabetes. The a2 isoform of Vacuolar ATPase and Cancer-Related Inflammation. V-ATPases in oral squamous cell carcinoma. Vacuolar H(+)-ATPase : functional mechanism and potential as a target for cancer chemotherapy. Vacuolar H+-ATPase Maintains Neural Stem Cells in the Developing Mouse Cortex. The relationship between glucose-induced calcium signaling and activation of the plasma membrane H+-ATPase in Saccharomyces cerevisiae cells.
£147.25
Pan Stanford Publishing Pte Ltd Cancer Genetics and Genomics for Personalized
Book SynopsisThis book covers almost all fields of cancer genetics and genomics for personalized medicine. Targeted therapy, or precision medicine, or personalized medicine is becoming a standard treatment for many diseases, including cancer. However, how much do we know about the personalized medicine approach? This lucid book helps undergraduate and graduate students, professional researchers, and clinicians to better understand the key concept of personalized medicine.The most up-to-date topics on personalized medicine in this book cover the recent trends in and updates on lung, gastric, liver, breast, and other types of cancers. Circulating tumor cell, cell-free circulating DNA, and microRNAs are discussed as new diagnostic and prognostic markers for cancer. The avatar mouse model is also discussed for maximizing treatment efficacy and prognosis prediction, and so is microenvironment as a drug resistance mechanism. With classical and new pathological approaches, the book provides a systemic overview of personalized immunotherapies and hyperthermic intraperitoneal chemotherapy, followed by new emerging fields of hereditary cancer, thereby equipping readers to eventually contribute in developing more advanced tools and therapies for curing cancer.Trade Review"Likely as the recent Cancer Moonshot Initiative highlights, this book well describes the importance of genetics/genomics-based personalized therapies to improve cancer treatments. This field is growing rapidly, and it is certain that many scientists should obtain professional knowledge of ‘cancer precision medicine’ to cure more cancer patients."—Prof. Yusuke Nakamura, University of Chicago, USA"At a time when an explosive increase in data has combined with rapidly evolving treatment paradigms, it has become particularly challenging for modern clinicians and medical researchers alike to put these advances in knowledge and practice into a proper context. It is in light of this predicament that this new volume edited by Dr. Il-Jin Kim provides a much-needed organization and harmonic understanding to the cacophony of information regarding the elusive realm of precision medicine. Covering an exhaustive array of topics ranging from the basic science of tumor modeling to the biology of tumor microenvironments to the horizon of cancer immunotherapy and advances for specific cancer types, this clearly written and very readable compilation provides a solid foundation for comprehending the rapidly advancing dawn of a truly new, personalized, precision approach to the treatment and, hopefully, the cure of cancer."—Prof. Michael J. Mann, University of California, San FranciscoTable of ContentsPersonalized Medicine for Cancer: Introduction and Overview of the Book. Personalized Medicine in Lung Cancer. Genome-Based Personalized Medicine in Liver Cancer. Applications of Circulating DNA Analysis in Personalized Medicine. Circulating Tumor Cells and Personalized Medicine. Mouse Models in Personalized Cancer Medicine. Tumor Microenvironment, Therapeutic Resistance, and Personalized Medicine. Personalized Immune Therapy. Hyperthermic Intraperitoneal Chemotherapy (HIPEC) for Peritoneal Malignancies. Personalized Medicine in Hereditary Cancer Syndromes. Pathology in the Era of Personalized Medicine. MicroRNAs in Human Cancers. Pharmacogenomics of Tamoxifen.
£102.60
The University of Chicago Press In Search of Cell History
Book SynopsisThe origin of cells remains one of the most fundamental problems in biology, one that over the past two decades has spawned a large body of research and debate. This book offers a comprehensive, impartial take on that research and the controversies that keep the field in turmoil.Trade Review"This book is a rare pleasure: a beautiful, rational, wise, and eloquent framing of life's greatest mysteries, what remains to be known, and how we might get there. It should be read by anyone who wonders, seriously, how we came to be. If it does not provide all the answers, that is because we honestly do not know." (Nick Lane, University College London)"
£96.90
The University of Chicago Press The Cell
Book Synopsis
£36.55
The University of Chicago Press The Third Lens
Book SynopsisAn analysis of the role of metaphor in shaping the work and findings of science, using cell biology as the central case study.
£76.00
The University of Chicago Press The Third Lens
Book SynopsisAn analysis of the role of metaphor in shaping the work and findings of science, using cell biology as the central case study.
£26.00
The University of Chicago Press Plant Allometry The Scaling of Form and Process
Book SynopsisAllometry, the study of the growth rate of an organism's parts in relation to the whole, has produced various results in research on animals. This text applies allometry to studies of the evolution, morphology, physiology and reproduction of plants.
£42.75
The University of Chicago Press The Evolutionary Origins of Life and Death
Book SynopsisTrade Review"That life is connected to death, and to evolution, is incontrovertible. But what might be the connection between death and evolution? That is, might death be an adaptation? That’s the startling conclusion reached by Durand in this brief volume, which combines philosophy, biological theory, and empirical science in often thrilling ways... Durand thinks big thoughts but is careful, in his prose, to involve readers whose interest in theoretical biology exceeds their grasp. A very important book. Highly recommended." * Choice Connect *"[The Evolutionary Origins of Life and Death] constitutes an impressive effort to integrate into a unified and coherent framework different and dispersed pieces of empirical work about how life and death emerged on Earth, and about how these two phenomena coevolved to become deeply entangled." * History and Philosophy of Life Sciences *“An eye opener for many readers, biologists, and others interested in visiting the frontiers of today’s conceptual thinking on evolution. This book, compact and simple but densely packed with information and ideas, presents the new edifice of evolutionary biology, a vibrant, progressing field that goes far beyond the confines of neo-Darwinism.” -- Eugene V. Koonin, National Center for Biotechnology Information, from the foreword“There is no question that Durand is deeply learned. The way that he lays out his argument linking life and death is certainly innovative and I am sure will attract attention. Insightful and stimulating, The Evolutionary Origins of Life and Death will forward discussion on important issues.” -- Michael Ruse, Florida State UniversityTable of ContentsForeword (by Eugene V. Koonin) Preface List of abbreviations An introduction to the scientific study of life and deathPart One: The origin of life 1 Philosophical considerations and the origin of life 2 The biotic world 3 The theory of life’s origins 4 Life at the very beginning I: the chemistry of the first biomolecules 5 Life at the very beginning II: the emergence of complex RNA molecules 6 The origin of life was an evolutionary transition in individuality 7 A synthesis for the origin of lifePart Two: The origin of death 8 Philosophical considerations and the origin of death 9 Observations of death 10 Mechanisms and measures of programmed cell death in the unicellular world 11 True PCD: when PCD is an adaptation 12 Ersatz PCD: the non-adaptive explanations for PCD 13 Programmed cell death and the levels of selection 14 A synthesis for the origin of programmed cell deathPart Three: Origins of life and death, and their coevolution 15 Group selection and the origins of life and death 16 Life and death coevolution, and the emergence of complexity Postface Additional notes Reference list Index
£87.40
The University of Chicago Press The Evolutionary Origins of Life and Death
Book SynopsisTrade Review"That life is connected to death, and to evolution, is incontrovertible. But what might be the connection between death and evolution? That is, might death be an adaptation? That’s the startling conclusion reached by Durand in this brief volume, which combines philosophy, biological theory, and empirical science in often thrilling ways... Durand thinks big thoughts but is careful, in his prose, to involve readers whose interest in theoretical biology exceeds their grasp. A very important book. Highly recommended." * Choice Connect *"[The Evolutionary Origins of Life and Death] constitutes an impressive effort to integrate into a unified and coherent framework different and dispersed pieces of empirical work about how life and death emerged on Earth, and about how these two phenomena coevolved to become deeply entangled." * History and Philosophy of Life Sciences *“An eye opener for many readers, biologists, and others interested in visiting the frontiers of today’s conceptual thinking on evolution. This book, compact and simple but densely packed with information and ideas, presents the new edifice of evolutionary biology, a vibrant, progressing field that goes far beyond the confines of neo-Darwinism.” -- Eugene V. Koonin, National Center for Biotechnology Information, from the foreword“There is no question that Durand is deeply learned. The way that he lays out his argument linking life and death is certainly innovative and I am sure will attract attention. Insightful and stimulating, The Evolutionary Origins of Life and Death will forward discussion on important issues.” -- Michael Ruse, Florida State UniversityTable of ContentsForeword (by Eugene V. Koonin) Preface List of abbreviations An introduction to the scientific study of life and deathPart One: The origin of life 1 Philosophical considerations and the origin of life 2 The biotic world 3 The theory of life’s origins 4 Life at the very beginning I: the chemistry of the first biomolecules 5 Life at the very beginning II: the emergence of complex RNA molecules 6 The origin of life was an evolutionary transition in individuality 7 A synthesis for the origin of lifePart Two: The origin of death 8 Philosophical considerations and the origin of death 9 Observations of death 10 Mechanisms and measures of programmed cell death in the unicellular world 11 True PCD: when PCD is an adaptation 12 Ersatz PCD: the non-adaptive explanations for PCD 13 Programmed cell death and the levels of selection 14 A synthesis for the origin of programmed cell deathPart Three: Origins of life and death, and their coevolution 15 Group selection and the origins of life and death 16 Life and death coevolution, and the emergence of complexity Postface Additional notes Reference list Index
£31.00
University of Notre Dame Press Stem Cell Research
Book SynopsisThis volume brings together essays by an internationally distinguished and diverse group of scholars. Contributors thoughtfully explore the ethical, public policy, and scientific implications of embryonic and adult stem cell research. Part one of the book offers a variety of scientific and public policy perspectives, including essays on stem cell plasticity and using umbilical cord blood as an alternative source of pluripotent stem cells. Part two vigorously examines the ethics of stem cell research and considers issues of social justice, morality, and public policy. Scientific alternatives, a natural law perspective regarding federal funding, and a discussion of the possible moral complicity of Catholic researchers are among the distinctive contributions made to the stem cell research debate by this collection. The objective and balanced discussions contained in this volume serve as an accessible introduction to the bioethical questions, issues, and problems surroundiTrade Review“This collection covers a broad range of scientific, ethical, and public policy issues. Some articles provide general background and information, while others make highly original contributions to the ongoing debate. Ethical issues are discussed knowledgeably from a variety of religious and philosophical perspectives. Thoughtful disagreements among authors illustrate the difficulty of reaching a consensus on public policy.” —Carol A. Tauer, Professor of Philosophy Emerita, The College of St. Catherine, St. Paul, MN"In the book, Stem Cell Research, Nancy Snow provides a collection of essays that discuss different aspects of the stem cell debate. This is an important endeavor. . ." —Dialogue". . . this collection of essays is sure to speak to readers on either side of this divisive issue." —NationalJournal.com"[Stem Cell Research] provides a useful primer for many. . . of the ethical issues presented by the embryonic stem cell debate." —The Quarterly Review of Biology“This collection is an important resource for any bioethics course.” —Theological Studies“. . . so volatile and dynamic are the variables in the debates over this type of research, it would be a mistake not to look upon it as having lasting value. The authors are attentive to cutting edge science to be sure, but they effectively raise up time-tested principles from biomedical ethics and the natural law tradition in an effort to weigh the pros and cons.” —Catholic Library World“Snow’s volume is earnest . . . a plea for ethically informed science.” —Times Literary Supplement“. . . the contributions are thought-provoking and the arguments need to be addressed by proponents of the research.” —Conscience“This volume is a collection of ten essays developed out of a 2001 conference . . . the gathering convened scientists and ethicists to discuss scientific, public policy, and ethical issues concerning human stem cell research . . . the essays offer a wide range of topics, analyses, and arguments, and, as a whole, exhibit some of the complexity of issues involved in discussing human stem cell research-human embryonic stem cell research in particular. As such, the volume is an excellent introduction to the nuanced contours of this contentious realm.” —Pro Ecclesia
£22.79
Springer-Verlag New York Inc. An Atlas of Histology
Book SynopsisBridging the gap between textbook diagrams and the complex reality of histological preparations, this magnificent atlas of human microanatomy is designed to help students understand the complex structures encountered when viewing microscopic sections of tissues. Instead of simply depicting an individual section, each drawing is a compilation of the key structures and features seen in many preparations from similar tissues or organs. Invaluable to students in a range of life science and medical disciplines including human and veterinary medicine, dentistry, mammalian biology, pharmacy, and nursing.Table of Contents1. Epithelial Tissue.- 2. Connective Tissue.- 3. Cartilage and Bone.- 4. Blood Cells and Hemopoietic Cells.- 5. Muscular Tissue.- 6. Nervous Tissue and Nervous System.- 7. Circulatory System.- 8. Lymphatic Organs.- 9. Respiratory System.- 10. Digestive System.- 11. Urinary System.- 12. Male Reproductive System.- 13. Female Reproductive System.- 14. Endocrine Organs.- 15. The Integument.- 16. The Eye.- 17. The Ear.- References.
£123.49
John Wiley & Sons Inc Biophysical Chemistry of Biointerfaces
Book SynopsisBiointerfaces are central to biology and medicine and crucial in research relating to implants, biosensors, drug delivery, proteomics, and many other fields.Trade Review"Ohshima (pharmaceutical science, Tokyo U. of Science) sets out a set of tools for discussing various phenomena at biological interfaces - such as cell surfaces - in terms of biophysical chemistry." (SciTech Book News, December 2010) Table of ContentsPreface xiii List of Symbols xv Part I Potential and Charge at Interfaces 1 1 Potential and Charge of a Hard Particle 3 1.1 Introduction 3 1.2 The Poisson-Boltzmann Equation 3 1.3 Plate 6 1.3.1 Low Potential 8 1.3.2 Arbitrary Potential: Symmetrical Electrolyte 8 1.3.3 Arbitrary Potential: Asymmetrical Electrolyte 13 1.3.4 Arbitrary Potential: General Electrolyte 14 1.4 Sphere 16 1.4.1 Low Potential 17 1.4.2 Surface Charge Density-Surface Potential Relationship: Symmetrical Electrolyte 18 1.4.3 Surface Charge Density-Surface Potential Relationship: Asymmetrical Electrolyte 21 1.4.4 Surface Charge Density-Surface Potential Relationship: General Electrolyte 22 1.4.5 Potential Distribution Around a Sphere with Arbitrary Potential 25 1.5 Cylinder 31 1.5.1 Low Potential 32 1.5.2 Arbitrary Potential: Symmetrical Electrolyte 33 1.5.3 Arbitrary Potential: General Electrolytes 34 1.6 Asymptotic Behavior of Potential and Effective Surface Potential 37 1.6.1 Plate 38 1.6.2 Sphere 41 1.6.3 Cylinder 42 1.7 Nearly Spherical Particle 43 References 45 2 Potential Distribution Around a Nonuniformly Charged Surface and Discrete Charge Effects 47 2.1 Introduction 47 2.2 The Poisson-Boltzmann Equation for a Surface with an Arbitrary Fixed Surface Charge Distribution 47 2.3 Discrete Charge Effect 56 References 62 3 Modified Poisson-Boltzmann Equation 63 3.1 Introduction 63 3.2 Electrolyte Solution Containing Rod-like Divalent Cations 63 3.3 Electrolyte Solution Containing Rod-like Zwitterions 70 3.4 Self-atmosphere Potential of Ions 77 References 82 4 Potential and Charge of a Soft Particle 83 4.1 Introduction 83 4.2 Planar Soft Surface 83 4.2.1 Poisson–Boltzmann Equation 83 4.2.2 Potential Distribution Across a Surface Charge Layer 87 4.2.3 Thick Surface Charge Layer and Donnan Potential 90 4.2.4 Transition Between Donnan Potential and Surface Potential 91 4.2.5 Donnan Potential in a General Electrolyte 92 4.3 Spherical Soft Particle 93 4.3.1 Low Charge Density Case 93 4.3.2 Surface Potential–Donnan Potential Relationship 95 4.4 Cylindrical Soft Particle 100 4.4.1 Low Charge Density Case 100 4.4.2 Surface Potential–Donnan Potential Relationship 101 4.5 Asymptotic Behavior of Potential and Effective Surface Potential of a Soft Particle 102 4.5.1 Plate 102 4.5.2 Sphere 103 4.5.3 Cylinder 104 4.6 Nonuniformly Charged Surface Layer: Isoelectric Point 104 References 110 5 Free Energy of a Charged Surface 111 5.1 Introduction 111 5.2 Helmholtz Free Energy and Tension of a Hard Surface 111 5.2.1 Charged Surface with Ion Adsorption 111 5.2.2 Charged Surface with Dissociable Groups 116 5.3 Calculation of the Free Energy of the Electrical Double Layer 118 5.3.1 Plate 119 5.3.2 Sphere 120 5.3.3 Cylinder 121 5.4 Alternative Expression for Fel 122 5.5 Free Energy of a Soft Surface 123 5.5.1 General Expression 123 5.5.2 Expressions for the Double-Layer Free Energy for a Planar Soft Surface 127 5.5.3 Soft Surface with Dissociable Groups 128 References 130 6 Potential Distribution Around a Charged Particle in a Salt-Free Medium 132 6.1 Introduction 132 6.2 Spherical Particle 133 6.3 Cylindrical Particle 143 6.4 Effects of a Small Amount of Added Salts 146 6.5 Spherical Soft Particle 152 References 162 Part II Interaction Between Surfaces 163 7 Electrostatic Interaction of Point Charges in an Inhomogeneous Medium 165 7.1 Introduction 165 7.2 Planar Geometry 166 7.3 Cylindrical Geometry 180 References 185 8 Force and Potential Energy of the Double-Layer Interaction Between Two Charged Colloidal Particles 186 8.1 Introduction 186 8.2 Osmotic Pressure and Maxwell Stress 186 8.3 Direct Calculation of Interaction Force 188 8.4 Free Energy of Double-Layer Interaction 198 8.4.1 Interaction at Constant Surface Charge Density 199 8.4.2 Interaction at Constants Surface Potential 200 8.5 Alternative Expression for the Electric Part of the Free Energy of Double-Layer Interaction 201 8.6 Charge Regulation Model 201 References 202 9 Double-Layer Interaction Between Two Parallel Similar Plates 203 9.1 Introduction 203 9.2 Interaction Between Two Parallel Similar Plates 203 9.3 Low Potential Case 207 9.3.1 Interaction at Constant Surface Charge Density 208 9.3.2 Interaction at Constant Surface Potential 211 9.4 Arbitrary Potential Case 214 9.4.1 Interaction at Constant Surface Charge Density 214 9.4.2 Interaction at Constant Surface Potential 224 9.5 Comparison Between the Theory of Derjaguin and Landau and the Theory of Verwey and Overbeek 226 9.6 Approximate Analytic Expressions for Moderate Potentials 227 9.7 Alternative Method of Linearization of the Poisson–Boltzmann Equation 231 9.7.1 Interaction at Constant Surface Potential 231 9.7.2 Interaction at Constant Surface Charge Density 234 References 240 10 Electrostatic Interaction Between Two Parallel Dissimilar Plates 241 10.1 Introduction 241 10.2 Interaction Between Two Parallel Dissimilar Plates 241 10.3 Low Potential Case 244 10.3.1 Interaction at Constant Surface Charge Density 244 10.3.2 Interaction at Constant Surface Potential 251 10.3.3 Mixed Case 252 10.4 Arbitrary Potential: Interaction at Constant Surface Charge Density 252 10.4.1 Isodynamic Curves 252 10.4.2 Interaction Energy 258 10.5 Approximate Analytic Expressions for Moderate Potentials 262 References 263 11 Linear Superposition Approximation for the Double-Layer Interaction of Particles at Large Separations 265 11.1 Introduction 265 11.2 Two Parallel Plates 265 11.2.1 Similar Plates 265 11.2.2 Dissimilar Plates 270 11.2.3 Hypothetical Charge 276 11.3 Two Spheres 278 11.4 Two Cylinders 279 References 281 12 Derjaguin’s Approximation at Small Separations 283 12.1 Introduction 283 12.2 Two Spheres 283 12.2.1 Low Potentials 285 12.2.2 Moderate Potentials 286 12.2.3 Arbitrary Potentials: Derjaguin’s Approximation Combined with the Linear Superposition Approximation 288 12.2.4 Curvature Correction to Derjaguin’ Approximation 290 12.3 Two Parallel Cylinders 292 12.4 Two Crossed Cylinders 294 References 297 13 Donnan Potential-Regulated Interaction Between Porous Particles 298 13.1 Introduction 298 13.2 Two Parallel Semi-infinite Ion-penetrable Membranes (Porous Plates) 298 13.3 Two Porous Spheres 306 13.4 Two Parallel Porous Cylinders 310 13.5 Two Parallel Membranes with Arbitrary Potentials 311 13.5.1 Interaction Force and Isodynamic Curves 311 13.5.2 Interaction Energy 317 13.6 pH Dependence of Electrostatic Interaction Between Ion-penetrable Membranes 320 References 322 14 Series Expansion Representations for the Double-Layer Interaction Between Two Particles 323 14.1 Introduction 323 14.2 Schwartz’s Method 323 14.3 Two Spheres 327 14.4 Plate and Sphere 342 14.5 Two Parallel Cylinders 348 14.6 Plate and Cylinder 353 References 356 15 Electrostatic Interaction Between Soft Particles 357 15.1 Introduction 357 15.2 Interaction Between Two Parallel Dissimilar Soft Plates 357 15.3 Interaction Between Two Dissimilar Soft Spheres 363 15.4 Interaction Between Two Dissimilar Soft Cylinders 369 References 374 16 Electrostatic Interaction Between Nonuniformly Charged Membranes 375 16.1 Introduction 375 16.2 Basic Equations 375 16.3 Interaction Force 376 16.4 Isoelectric Points with Respect To Electrolyte Concentration 378 Reference 380 17 Electrostatic Repulsion Between Two Parallel Soft Plates After Their Contact 381 17.1 Introduction 381 17.2 Repulsion Between Intact Brushes 381 17.3 Repulsion Between Compressed Brushes 382 References 387 18 Electrostatic Interaction Between Ion-Penetrable Membranes In a Salt-free Medium 388 18.1 Introduction 388 18.2 Two Parallel Hard Plates 388 18.3 Two Parallel Ion-Penetrable Membranes 391 References 398 19 van der Waals Interaction Between Two Particles 399 19.1 Introduction 399 19.2 Two Molecules 399 19.3 A Molecule and a Plate 401 19.4 Two Parallel Plates 402 19.5 A Molecule and a Sphere 404 19.6 Two Spheres 405 19.7 A Molecule and a Rod 407 19.8 Two Parallel Rods 408 19.9 A Molecule and a Cylinder 408 19.10 Two Parallel Cylinders 410 19.11 Two Crossed Cylinders 412 19.12 Two Parallel Rings 412 19.13 Two Parallel Torus-Shaped Particles 413 19.14 Two Particles Immersed In a Medium 417 19.15 Two Parallel Plates Covered with Surface Layers 418 References 419 20 DLVO Theory of Colloid Stability 420 20.1 Introduction 420 20.2 Interaction Between Lipid Bilayers 420 20.3 Interaction Between Soft Spheres 425 References 429 Part III Electrokinetic Phenomena at Interfaces 431 21 Electrophoretic Mobility of Soft Particles 433 21.1 Introduction 433 21.2 Brief Summary of Electrophoresis of Hard Particles 433 21.3 General Theory of Electrophoretic Mobility of Soft Particles 435 21.4 Analytic Approximations for the Electrophoretic Mobility of Spherical Soft Particles 440 21.4.1 Large Spherical Soft Particles 440 21.4.2 Weakly Charged Spherical Soft Particles 444 21.4.3 Cylindrical Soft Particles 447 21.5 Electrokinetic Flow Between Two Parallel Soft Plates 449 21.6 Soft Particle Analysis of the Electrophoretic Mobility of Biological Cells and Their Model Particles 454 21.6.1 RAW117 Lymphosarcoma Cells and Their Variant Cells 454 21.6.2 Poly(N-isopropylacrylamide) Hydrogel-Coated Latex 455 21.7 Electrophoresis of Nonuniformly Charged Soft Particles 457 21.8 Other Topics of Electrophoresis of Soft Particles 463 References 464 22 Electrophoretic Mobility of Concentrated Soft Particles 468 22.1 Introduction 468 22.2 Electrophoretic Mobility of Concentrated Soft Particles 468 22.3 Electroosmotic Velocity in an Array of Soft Cylinders 475 References 479 23 Electrical Conductivity of a Suspension of Soft Particles 480 23.1 Introduction 480 23.2 Basic Equations 480 23.3 Electrical Conductivity 481 References 484 24 Sedimentation Potential and Velocity in a Suspension of Soft Particles 485 24.1 Introduction 485 24.2 Basic Equations 485 24.3 Sedimentation Velocity of a Soft Particle 490 24.4 Average Electric Current and Potential 490 24.5 Sedimentation Potential 491 24.6 Onsager’s Reciprocal Relation 494 24.7 Diffusion Coefficient of a Soft Particle 495 References 495 25 Dynamic Electrophoretic Mobility of a Soft Particle 497 25.1 Introduction 497 25.2 Basic Equations 497 25.3 Linearized Equations 499 25.4 Equation of Motion of a Soft Particle 501 25.5 General Mobility Expression 501 25.6 Approximate Mobility Formula 503 References 506 26 Colloid Vibration Potential in a Suspension of Soft Particles 508 26.1 Introduction 508 26.2 Colloid Vibration Potential and Ion Vibration Potential 508 References 513 27 Effective Viscosity of a Suspension of Soft Particles 515 27.1 Introduction 515 27.2 Basic Equations 516 27.3 Linearized Equations 518 27.4 Electroviscous Coefficient 520 27.5 Approximation for Low Fixed-Charge Densities 523 27.6 Effective Viscosity of a Concentrated Suspension of Uncharged Porous Spheres 527 Appendix 27a 530 References 531 Part IV other Topics 533 28 Membrane Potential and Donnan Potential 535 28.1 Introduction 535 28.2 Membrane Potential and Donnan Potential 535 References 541 Index 543
£127.76
John Wiley & Sons Inc Plant Cell Culture Essential Methods
Book SynopsisThe ability to culture cells is fundamental for mass propagationand as a baseline for the genetic manipulation of plant nuclei andorganelles.Trade Review"In summary, this book is important for plant scientists as it thoroughly covers elementary technologies, providing the tools for comprehensive plant research." (The Quarterly Review of Biology, 1 September 2011) "For anyone working in the area of plant tissue culture, micro-propagation or transformation this book is essential reading." (The Journal of Experimental Agriculture, 1 October 2011)Table of ContentsPreface. Contributors. 1 Plant Micropropagation (Ivan Iliev, Alena Gajdosova, Gabriela Libiakova, Shri Mohan Jain). 1.1 Introduction. 1.2 Methods and approaches. 1.2.1 Explants and their surface disinfection. 1.2.2 Culture media and their preparation. 1.2.3 Stages of micropropagation. 1.2.4 Techniques of micropropagation. 1.3 Troubleshooting. References. 2 Thin Cell Layers: The Technique (Jaime A. Teixeira da Silva and Michio Tanaka). 2.1 Introduction. 2.2 Methods and approaches. 2.2.1 TCL. 2.2.2 Choice of material: Cymbidium hybrid. 2.3 Troubleshooting. 2.3.1 General comments. References. 3 Plant Regeneration – Somatic Embryogenesis (Kim E. Nolan, Ray J. Rose). 3.1 Introduction. 3.2 Methods and approaches. 3.2.1 Selection of the cultivar and type of explant. 3.2.2 Culture media. 3.2.3 Preparation of culture media. 3.2.4 Sterilization of tissues and sterile technique. 3.2.5 Culture and growth of tissue. 3.2.6 Culture and induction of somatic embryos. 3.2.7 Embryo development. 3.2.8 Transfer to soil – the final stage of regeneration. 3.3 Troubleshooting. References. 4 Haploid Plants (Sant S. Bhojwani and Prem K. Dantu). 4.1 Introduction. 4.2 Methods and approaches. 4.2.1 Androgenesis. 4.2.2 Diploidization. 4.3 Troubleshooting. References. 5 Embryo Rescue (Traud Winkelmann, Antje Doil, Sandra Reinhardt and Aloma Ewald). 5.1 Introduction. 5.2 Methods and approaches. 5.2.1 Identification of the time and type of barrier in hybridization. 5.2.2 Isolation of plant material after fertilization. 5.2.3 Culture conditions and media. 5.2.4 Confirmation of hybridity and ploidy. 5.2.5 Conditions for regeneration of embryos to plants. 5.3 Troubleshooting. References. 6 In vitro Flowering and Seed Set: Acceleration of Generation Cycles (Sergio J. Ochatt and Rajbir S. Sangwan). 6.1 Introduction. 6.2 Methods and approaches. 6.2.1 Protein legumes [7]. 6.2.2 Arabidopsis thaliana [13]. 6.3 Troubleshooting. References. 7 Induced Mutagenesis in Plants Using Physical and Chemical Agents (Chikelu Mba, Rownak Afza, Souleymane Bado and Shri Mohan Jain). 7.1 Introduction. 7.2 Methods and approaches. 7.2.1 Determination of the optimal doses of mutagens for inducing mutations. 7.3 Troubleshooting. 7.3.1 Factors influencing the outcome of mutagenesis using chemical mutagens. 7.3.2 Factors influencing the outcome of mutagenesis using physical mutagens. 7.3.3 Facts about induced mutations. References. 8 Cryopreservation of Plant Germplasm (E.R. Joachim Keller and Angelika Senula). 8.1 Introduction. 8.2 Methods and approaches. 8.2.1 Main principles. 8.2.2 Slow (two-step) freezing. 8.2.3 Vitrification. 8.2.4 Encapsulation–dehydration. 8.2.5 DMSO droplet freezing. 8.2.6 Combined methods. 8.2.7 Freezing of cold-hardened buds. 8.2.8 Freezing of orthodox seeds. 8.2.9 Freezing of pollen and spores. 8.3 Troubleshooting. References. 9 Plant Protoplasts: Isolation, Culture and Plant Regeneration (Michael R. Davey, Paul Anthony, Deval Patel and J. Brian Power). 9.1 Introduction. 9.2 Methods and approaches. 9.2.1 Protoplast isolation. 9.2.2 Protoplast culture. 9.3 Troubleshooting. References. 10 Protoplast Fusion Technology – Somatic Hybridization and Cybridization (Jude W. Grosser, Milica Calovic and Eliezer S. Louzada). 10.1 Introduction. 10.2 General applications of somatic hybridization. 10.3 Methods and approaches. 10.4 Troubleshooting. References. 11 Genetic Transformation – Agrobacterium (Ian S. Curtis). 11.1 Introduction. 11.2 Methods and approaches. 11.2.1 Agrobacterium as a natural genetic engineer. 11.2.2 Vector systems for transformation. 11.2.3 Inoculation procedures. 11.3 Troubleshooting. References. 12 Genetic Transformation – Biolistics (Fredy Altpeter and Sukhpreet Sandhu). 12.1 Introduction. 12.2 Methods and approaches. 12.2.1 Biolistic technology. 12.2.2 Optimization of gene delivery parameters. 12.2.3 Target tissues. 12.2.4 Reporter gene assays. 12.2.5 Selection and plant regeneration. 12.3 Troubleshooting. References. 13 Plastid Transformation (Bridget V. Hogg, Cilia L.C. Lelivelt, Aisling Dunne, Kim-Hong Nguyen and Jacqueline M. Nugent). 13.1 Introduction. 13.2 Methods and approaches. 13.2.1 Principles of plastid transformation. 13.2.2 Biolistic-mediated plastid transformation. 13.2.3 PEG-mediated plastid transformation. 13.2.4 Identification and characterization of transplastomic plants. 13.3 Troubleshooting. 13.3.1 Biolistic-mediated transformation. 13.3.2 PEG-mediated transformation. References. 14 Molecular Characterization of Genetically Manipulated Plants (Cristiano Lacorte, Giovanni Vianna, Francisco J.L. Aragao and El´ıbio L. Rech). 14.1 Introduction. 14.2 Methods and approaches. 14.2.1 Plant DNA extraction. 14.2.2 Polymerase chain reaction. 14.2.3 Southern blot technique. 14.2.4 Analysis of the integration site: inverse PCR (iPCR) and thermal asymmetric interlaced PCR (Tail-PCR). 14.3 Troubleshooting. References. 15 Bioreactors (Spiridon Kintzios). 15.1 Introduction. 15.2 Methods and approaches. 15.2.1 Medium scale disposable or semidisposable airlift reactors. 15.2.2 The RITA temporary immersion reactor. 15.2.3 The LifeReactor. 15.2.4 Immobilized cell bioreactors. 15.2.5 Mini-bioreactors. 15.3 Troubleshooting. References. 16 Secondary Products (Kexuan Tang, Lei Zhang, Junfeng Chen, Ying Xiao, Wansheng Chen and Xiaofen Sun). 16.1 Introduction. 16.2 Methods and approaches. 16.2.1 Plant cell cultures. 16.2.2 Scale-up and regulation of secondary metabolite production. 16.2.3 Detection of secondary products. 16.3 Troubleshooting. References. 17 Plant Cell Culture – Present and Future (Jim M. Dunwell). 17.1 Introduction. 17.2 Micropropagation. 17.3 Embryogenesis. 17.3.1 Background. 17.3.2 Commercial exploitation of somatic embryos. 17.3.3 Molecular aspects of somatic embryogenesis. 17.3.4 Microspore derived embryos. 17.4 Haploid methodology. 17.4.1 Haploids and their exploitation. 17.4.2 Induction of haploid plants. 17.4.3 Molecular aspects of haploid induction from microspores. 17.4.4 Ab initio zygotic-like embryogenesis from microspores. 17.5 Somaclonal variation. 17.6 Transgenic methods. 17.6.1 Background. 17.6.2 Regeneration and transformation techniques. 17.6.3 Chloroplast transformation. 17.6.4 Biopharming. 17.7 Protoplasts and somatic hybridization. 17.8 Bioreactors. 17.8.1 Production of plant products. 17.8.2 Production of pharmaceuticals. 17.8.3 Production of food ingredients. 17.8.4 Production of cosmetics. 17.8.5 Analytical methodology. 17.9 Cryopreservation. 17.10 Intellectual property and commercialization. 17.10.1 Background. 17.10.2 Sources of patent and other relevant information. 17.11 Conclusion. References. Index.
£77.36
John Wiley & Sons Inc Culture of Hematopoietic Cells
Book SynopsisThis guide provides a comprehensive synthesis of proven methods for the culture and experimental manipulation of haemopoietic cells. Covering a range of human and mouse cell types, and featuring step-by-step laboratory procedures, it gives detailed instructions for reagent preparation.Table of ContentsPartial table of contents: PRIMITIVE PROGENITOR ASSAYS. Cobblestone Area Forming Cell (CAFC) Assay (R. Ploemacher). Assay for Murine Blast Cell Colonies (N. Katayama & M.Ogawa). Serum-Deprived Cultures of Primary Hematopoietic Cells (G.Migliaccio & A. Migliaccio). COLONY-FORMING ASSAYS FOR MALIGNANT CELLS. Colony-Forming Assays for Acute Myeloid Leukemia (S. Rogers, etal.). LONG-TERM ASSAYS AND IMMORTALIZATION. Long-Term Culture of Human Myeloid Cells (H. Sutherland & C.Eaves). Immortalization of Hematopoietic Cells (C. MacDonald). PURIFICATION OF PROGENITOR CELLS. Purification of Murine Granulocyte-Macrophage Progenitor Cells(CFU-GM) Using Counterflow Centrifugal Elutriation (S. Cooper &H. Broxmeyer). OTHER SYSTEMS AND ASSAYS. Culture of Human and Mouse Bone Marrow Stromal Cells (S. Perkins& R. Fleischman). List of Suppliers. Index.
£171.86
Wiley Medical Biofilms v2 Detection Prevention and
Book SynopsisBiofilms are formed by microorganisms growing on surfaces and comprise a series of microcolonies interspersed with spaces through which fluids and other microorganisms move. This book focuses on medical biofilms, covering information on the problems of biofilms, how to detect them and how to control their presence.Table of ContentsContributors. Preface. Glossary. 1. Microbial Biofilms in Medicine (J, Jass, S. Surman and J. Walker). 2. Biofilms Associated with Medical Devices and Implants. Problems of Biofilms Associated with Medical Devices and Implants (R. Donlan). Pathogenesis and Detection of Biofilm Formation, on Medical Impants (C. Von Eiff and G. Peters). Control of Biofilms Associated With Implanted Medical Devices (P. Gilbert, et al.). 3. Microbial Adhesion and Biofilm Formation on Tissue Surfaces. Biofilm-Related Infections on Tissue Surfaces (S. Wai, Y. Mizunoe and J. Jass). Interaction of Biofilms with Tissues (M. Olson, H. Ceri and D. Morck). Control of Microbial Adhesion and Biofilm Formation on Tissue Surfaces (G. Reid, et al. ). 4. Dental Plaque and Bacterial Colonization of Dental Materials. Dental Plaque and Bacterial Colonization (D. Spratt). Detection of Microorganisms in Dental Plaque (D. Dymock). Control of Dental Plaque (R. Sammons). 5. Biofilms Past, Present and Future-New Methods and Control Strategies in Medicine (J. Walker, S. Surman and J. Jass). Index.
£145.76
John Wiley and Sons Ltd Molecular Methods in Ecology
Book Synopsis* Provides up-to-date summaries of the latest molecular approaches in this rapidly expanding field. * Gives guidance on the appropriate choice of methods for particular problems in ecology, and their strengths and limitations.Table of Contents1. Molecular Ecology (A J Baker). 2. General Molecular Biology (R E Carter). 3. Polymerase Chain Reaction (T P Birt & A J Baker). 4. Protein Electrophoresis (A J Baker). 5. Solution DNA-DNA Hybridisation (A H Bledsoe & F H Sheldon). 6. DNA Fingerprinting using minisatellite probes (R E Carter). 7. Mitochondrial DNA (E Randi). 8. Characterization and Evolution of Major Histocompatibility Complex (Mhc) Geners in Non-Model Organisms, with Examples from Birds (S V Edwards, J Nusser & J Gasper). 9. DNA-fragment markers in plants (C Ritland & K Ritland). 10. Microsatellites: Evolutionary and Methodological Background and Empirical Applications at individual, population and phylogenetic levels (K T Scribner & J M Pearce). 11. Introns (V Friesen). 12. Sex Identification using DNA markers (R Griffiths)
£98.06
John Wiley and Sons Ltd Route Maps in Gene Technology
Book SynopsisGene-based techniques have revolutionized our understanding of biology and had a direct impact on many aspects of our lives, from clinical medicine to agriculture. This book allows the reader to choose from pathways in discovering the concepts of molecular biology, the principles of a recombinant DNA technology and its key methods.Table of ContentsPreface. About the Route Maps format. The Concept Of Genes Is Developed. Genes Are Located To Chromosomes. Genes Are Composed Of DNA. The Chemical Building Blocks Of Nucelic Acids. Formation Of The DNA Double Helix. Packaging Of DNA Within Cells. Chromatin Structure And The Functional Activity Of Genes. Types And Functions Of DNA-Protein Interactions. Organization Of Genomes Into Multiple Chromosomes. Distribution Of Nucleic Acids Within Eukaryotic Cells. Types Of RNA Molecules. The Anatomy Of Eukaryotic Chromosomes. The Organisation Of Genes Within Chromosomes. The Molecular Anatomy Of Eukaryotic Genes. Chromosome Aberrations And Human Disease. Types Of Mutations And Their Effects. Forms Of Chemically Altered DNA; DNA Repair Mechanisms. Linkage Analysis. Pedigree Analysis And Modes Of Inheritance. Genes Dictate The Nature Of Proteins. The Nature Of The Genetic Code. Transcription: Forming Genetic Messages. Post-Transcriptional Processing Of Messenger RNA. Transfer And Ribosomal RNA Processing/Modification. Mechanisms Regulating Gene Expression. Transcriptional Regulatory Sequences. Operons And Prokaryotic Control Of Gene Expression. Transcription Factors And Gene Expression. In Vivo Translation: Decoding Genetic Messages. Sequences Involved In Cellular Protein Targeting. Eukaryotic Cell Division: Mitosis And Meiosis. Molecular Mechanisms Of Cell Cycle Control. Genetic Recombination Mechanisms. Gene Transfer During Bacterial Reproduction. Transposable Genetic Elements: Transposons. In Vivo DNA Replication. Genetic Control Of Development. The Natural Biology Of Bacteriophages. Bacteriophage Genetics. Recombinant DNA Technology. Enzymes Commonly Used In Molecular Biology Methods. Restriction Endonucleases. Restriction Fragment Length Polymorphisms. Isolation Of Nucleic Acids From Cells And Tissues. Visualising Mucleic Acids. Electrophoresis Of Nucleic Acids. In Vitro Hybridisation. Types Of Hybridisation Assay Formats. Southern Blotting; In Situ Hybridisation. Measuring Transcriptional Activity Via Messenger RNA. Converting Messenger RNA Into Complementary DNA (Cdna). Methods For Determining DNA Nucleotide Sequences. The Polymerase Chain Reaction. Alternatives To PCR-Based In Vitro DNA/RNA Amplification. In Vitro Translation Methods. Types And Methods Of Gene Probe Generation. Chemical Synthesis Of Oligonucleotides. Types And Applications Of Nucleotide Analogues. Methods For Labelling Gene Probes. Fundamental Principles Of Cloning. The Nature Of Cloning Vectors. Inserting Foreign DNA Into Vectors. The Development Of Bacteriophage Vectors. Plasmids: Development As Clonign Vectors. Yeast-Derived Plasmid Vectors. Phagemids: Hybrid Phage And Plasmid Vectors. Vectors For Use In Plant And Animal Cells. Delivering DNA Into Cells; Principal Genomic And Cdna Cloning Strategies. Strategies For Identifying Desirable Recombinant Clones. Gene Mapping Techniques. Detecting DNA-Protein Interaction Sites. Detecting Promoter And Enhancer Sequences. Methods For Identifying Protein Encoding Sequences. Genetic Fingerprinting. Analysing Ancient Dnas. In Vitro Mutagenesis Methods. Genetically Modified Micro-Organisms. Genetically Engineered Plants. Genetically Engineered Animals. Molecular Techniques In Prenatal Diagnosis. The Genetics Of Cystic Fibrosis. The Dystrophin Gene And Muscular Dystrophies. Identifying The Gene For Huntingdon's Disease. Lipoprotein Genes And Heart Disease. The Detection Of Microbial Infections. Molecular Biology Of Human Immunodeficiency Virus And AIDS. Engineering Microbial Bioluminescence. Recombinant DNA Techniques In Vaccine Development. Receptors And Cellular Signalling Mechanisms. Oncogenes And The Molecular Basis Of Cancer. Molecular Diagnosis And Therapy Of Cancers. Drug Development Using Recombinant DNA Approaches. Protein Engineering. Immunoglobulin Genetics. Genetic Engineering Of Recombinant Antibodies. Current Approaches To Gene Therapy. The Human Genome Mapping Project. Bibliography. Index.
£84.50
Harvard University Press The Generation of Diversity
Book SynopsisThis book is an intellectual history of the major theoretical problem in immunology and its resolution in the postâWorld War II period. In recent years immunology has been one of the most excitingâand successfulâfields of biomedical research; this book provides essential background for understanding the conceptual conflicts occurring in the field.Trade ReviewScott Podolsky and Alfred Tauber have written a dense and exhaustive scholarly treatise about the history of [the generation of diversity]. They explore the intellectual setting in which the Tonegawa experiment was performed by reviewing original literature and interviewing the major actors in the drama...Everyone interested in the history of immunology and genetics should read this book. -- Fred S. Rosen * Nature *The Generation of Diversity is a remarkable mixture of formal history, literature review, and 'immunophilosophy.' It traces the origins of Burnet's clonal selection theory as a central paradigm of contemporary immunology, then reconciles that theory with the discoveries of the molecular era, and concludes with consideration of 'the immune self'...The authors' meticulously detailed background to the experimental work is a tour de force, which one would never expect to be attempted again, and it stands as a vibrant testament to an extraordinary era for immunology. -- Ian R. Mackay * Science *This book is thoroughly researched, well written, and obviously intended to be definitive with respect to clonal selection and the generation of antibody diversity… Despite, or perhaps because of, the great amount of detail in this book, I enjoyed reading it… For an immunologist, it is satisfying to have the history of two major areas of research recapitulated. This book includes many details I missed earlier or had forgotten, and it is of interest to compare the authors’ assignment of priorities for correct conclusions with those of the principals, as expresses in their own reviews of the topic. This book will be an essential part of the collection of anyone with an interest in the history of immunology. -- Alfred Nisonoff * New England Journal of Medicine *This scholarly historiography is a must for those with more than a passing interest in immunology. Nine well-structured chapters cover the issues that have led to a general acceptance of the clonal selection theory and the establishment of molecular immunology as the arbiter of current thinking… Scott Podolsky and Alfred Tauber’s conceptual grasp of the ideas is anchored by a thorough explanation of critical experiments. They bring an almost extravagant degree of research, and attention to every argument, rendering the finished product as readable as a good novel. -- Richard Lake * Times Higher Education Supplement *Several recent studies have shown the central role of techniques, instruments, reagents and experimental systems in the ‘molecularization of biology and medicine’, first by focusing on the structure of proteins, then on the structure of nucleic acids (DNA and RNA). The Generation of Diversity is a fascinating account of how exactly this change took place in immunology. The book’s main strength [is] telling a very detailed story of a transformation of a single domain of scientific inquiry. -- Ilana Löwy * Medical History [UK] *A balanced and lucid conceptual history of postwar immunology, documenting one of its most fundamental transformations. This is an important and original contribution to the history of twentieth century biology. -- Angela Creager, Princeton UniversityTable of ContentsA conceptual orientation; the Burnet legacy; CST and molecular immunology - a dogmatic alliance; interlude - the conventional history; germline, somatic mutation and recombinatorial models - 1960-1974; immunobiological theories of antibody diversity; from protein to DNA; the recombinant revolution; interlude - a historigraphic reappraisal; heavy chain diversity and the molecular finale; an accounting; the fate of the immune self.
£37.36
Harvard University Press Cancer Stem Cells
Book SynopsisA new therapeutic strategy could break the stalemate in the war on cancer by targeting not all cancerous cells but the small fraction that lie at the root of cancers. Lucie Laplane offers a comprehensive analysis of cancer stem cell theory, based on an original interdisciplinary approach that combines biology, biomedical history, and philosophy.Trade ReviewLaplane places us right in the center of an urgently important discussion of cancer stem cells and the therapies that should accompany different theories. Her fresh philosophical perspective introduces us to a science in process, where the outcome is unknown and even the terms of debate remain contested. -- Jane Maienschein, author of Embryos under the MicroscopeIf you had doubts about the utility of philosophy for science, read this book. Its lucid exploration of stem cells will convince you that even cancer therapy can benefit from the lights of philosophy. -- Michel Morange, author of A History of Molecular BiologyBuilds a much broader framework for understanding the biology of stem cells of all types…Laplane’s rigorous analyses unveil deep semantic and conceptual problems in the field…Laplane’s stemness framework should be of great value…A philosopher may indeed have straightened out the stem-cell field. -- Hans Clever * Nature *
£31.41
Harvard University Press Body Messages
Book SynopsisWhether classified as regulators of inflammation, metabolism, or other functions, a distinctive set of molecules enables the body to convey information from one cell to another. Giamila Fantuzzi offers a primer on molecular mediators that coordinate complex bodily processes, and explores the consequences of their discovery for modern medicine.Trade ReviewFrom the way our brain thinks to how our body fights germs, the wonders of human life are fundamentally about how our cells and tissues interact. This book is a tour de force about how this communication happens. Examined with passion and insight, a wondrous and important story unfolds about how the human body really works above and beyond the level of individual genes and cells. -- Daniel M. Davis, author of The Compatibility GeneThe scholarship in the book is truly impressive. The interviews are revealing portraits of accomplished scientists. The writing style is lucid and purged of jargon so as to be intelligible to non-scientists. All in all this is a valuable and eminently readable record of the birth and development of a field that has had a dramatic impact on human health. -- Scott Durum, Center for Cancer Research, National Cancer InstituteReading this work will keep the reader current on a substantial number of recent, important studies on cell-to-cell information transfer. The reader will enjoy the personal essays and then want to read more about the scientific contributions. It is fascinating to find a work that is genuinely informative and also a joy to read. No matter the nature of the reader’s background, he or she will learn an immense amount of information from this book. -- F. W. Yow * Choice *
£31.41
Harvard University Press Adaptive Oncogenesis
Book SynopsisJames DeGregori proposes a way of thinking about cancer as a disease of evolutionone in which mutated cells outcompete healthy cells in the ecosystem of the body's tissues. By tying cancer's progression to natural selection and evolved strategies for reproductive success, his theory goes far in explaining who gets cancer, when it appears, and why.Trade ReviewWith engaging prose and an authoritative review of new research, Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us corrects the fundamental attribution error that has focused cancer research on malignant cells and their genes. Adaptive oncogenesis, or ‘EcoOncogenesis,’ shows that the ecosystems surrounding cells are equally important, responsible for creating selection forces that speed or slow the evolution of cancer. With huge implications for prevention and treatment, this book is required reading for cancer researchers and clinicians, and a pleasure read for anyone who appreciates fascinating new science. -- Randolph Nesse, Center for Evolution and Medicine at Arizona State UniversityDeGregori’s vision of cancer as an adaptive ecosystem is insightful and cogent. Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us, an evolutionary explanation for the risk of cancer as we age, is a provocative and refreshing revision of the prevailing gene-centric paradigm. -- Mel Greaves, The Institute of Cancer ResearchTo understand how and why tumors progress, James DeGregori argues that we must apply evolutionary principles to cancer. This book is a seamless discussion of natural evolutionary processes, ranging from longevity in rodents to bacterial antibiotic resistance, and drawing parallels to tumorigenesis. It is a must-read for everyone who wants to understand tumor evolution. -- Kornelia Polyak, Dana-Farber Cancer InstituteTo paraphrase Dobzhansky, cancer only makes sense in the light of evolution. In Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us, DeGregori challenges the commonly held view that it takes time for a normal cell to mutate into a cancer cell. He makes the compelling case that the ability to select against cancer becomes weaker as an individual gets older and the odds of reproduction decline. -- Angelika Amon, Koch Institute for Integrative Cancer Research at MITAt every point in our lives, the body holds the mutations necessary to cause cancer; why then does cancer develop primarily at certain times or after specific exposures? With crisp thinking and engaging prose, James DeGregori’s surprisingly readable book argues that cancer is a disease caused by damaged tissues rather than gene mutations. The implications of this revolutionary work have the potential to change the focus of cancer research, and especially research on cancer prevention, with emphasis on using the body’s own calculus of natural selection to keep cancers in check. -- Garth Sundem, author of Brain Trust: 93 Top Scientists Reveal Lab-Tested Secrets to Surfing, Dating, Dieting, Gambling, Growing Man-Eating Plants, and More!Adaptive Oncogenesis: A New Understanding of How Cancer Evolves inside Us is a highly readable and entertaining book, offering a fascinating new look at cancer through an evolutionary and ecological lens. With novel insights and thoughtful observations, James DeGregori guides his audience through the promise of new ideas, examining novel applications of Darwin’s principles as well as modern technological advancements connected to cancer biology and treatment. -- Robert A. Gatenby, Moffitt Cancer CenterA fresh view on how cancer originates and evolves, challenging the mutation-centric dogma of cancer genesis…An important book, accessible to general readers, teachers, and nonexpert biologists, and a must read for medical doctors and drug developers aiming to design effective cancer therapies, as it introduces the fundamental concept of evolution and tissue homeostasis into therapy design. -- Miguel Costa Coelho * Quarterly Review of Biology *
£34.81
Wiley Basic Molecular and Cell Biology
Book SynopsisThis third, fully revised edition, brings the reader up to date with recent advances made in the study of disease at the molecular and cellular level, and examines the new possibilities for treatment. Subjects covered include methods used in molecular medicine and the polymerase chain reaction.Table of ContentsMolecular and cell biology in clinical medicine; methods in molecular medicine; the polymerase chain reaction - a tool for molecular medicine; an introduction to cells; stem cells in normal growth and disease; cell reproduction; apoptosis (programmed cell death); cell to cell and cell to matrix adhesion; how do receptors at the cell surface transmit signals to the cell interior?; membrane traffic, from cell to clinic; cytoskeleton and disease; the cell nucleus' gene regulation and transcription factors; genes and cancer; human congenital malformations - insights from molecular genetics; molecular genetics of common diseases; impact of molecular biology on clinical genetics; monoclonal antibodies in medicine; production and use of therapeutic agents; gene therapy.
£42.70
John Wiley and Sons Ltd Plant Tropisms
Book SynopsisTropisms, the defined vectorial stimuli, such as gravity, light, touch, humidity gradients, ions, oxygen, and temperature, which provide guidance for plant organ growth, is a rapidly growing and changing field. The last few years have witnessed a true renaissance in the analysis of tropisms.Table of ContentsContributors. Preface. 1. Mechanisms of Gravity Perception in Higher Plants: Aline H. Valster and Elison B. Blancaflor. 1.1 Introduction. 1.2 Identification and characterization of gravity perception sites in plant organs. 1.2.1 Roots. 1.2.2 Hypocotyls and inflorescence stems (dicotyledons). 1.2.3 Cereal pulvini (monocotyledons). 1.3 The Starch-statolith hypothesis. 1.3.1 A variety of plant organs utilize sedimenting amyloplasts to sense gravity. 1.3.2 Amyloplast sedimentation is influenced by the environment and developmental stage of the plant. 1.4 The gravitational pressure model for gravity sensing. 1.5 The cytoskeleton in gravity perception. 1.6 Concluding remarks and future prospects. 1.7 Acknowledgment. 1.8 Literature Cited. 2. Signal Transduction in Gravitropism: Benjamin R. Harrison, Miyo T. Morita, Patrick H. Masson and Masao Tasaka. 2.1 Introduction. 2.2 Gravity signal transduction in roots and above-ground organs. 2.2.1 Do mechano-sensitive ion channels function as gravity receptors?. 2.2.2 Inositol 1,4,5 trisphosphate seems to function in gravity signal transduction. 2.2.3 Do pH changes contribute to gravity signal transduction?. 2.2.4 Proteins implicated in gravity signal transduction. 2.2.5 Global ‘-omic’ approaches to the study of root gravitropism. 2.2.6 Re-localization of auxin transport facilitators or activity regulation?. 2.2.7 Could cytokinin also contribute to the gravitropic signal?. 2.3 Gravity signal transduction in organs that do not grow vertically. 2.4 Acknowledgments. 2.5 Cited Literature. 3. Auxin Transport and the Integration of Gravitropic Growth: Gloria K. Muday and Abidur Rahman. 3.1 Introduction to auxins. 3.2 Auxin transport and its role in plant gravity response. 3.3 Approaches to Identify Proteins that Mediate IAA Efflux. 3.4 Proteins that Mediate IAA Efflux. 3.5 IAA influx carriers and their role in gravitropism. 3.6 Regulation of IAA efflux protein location and activity during gravity response. 3.6.1 Mechanisms that may control localization of IAA efflux carriers. 3.6.2 Regulation of IAA efflux by synthesis and degradation of efflux carriers. 3.6.3 Regulation of auxin transport by reversible protein phosphorylation. 3.6.4 Regulation of auxin transport by flavonoids. 3.6.5 Regulation of auxin transport by other signaling pathways. 3.6.6 Regulation of gravity response by ethylene. 3.7 Overview of the mechanisms of auxin induced growth. 3.8 Conclusions. 3.9 Acknowledgements. 3.10 Cited Literature. 4. Phototropism and its Relationship to Gravitropism: Jack L. Mullen and John Z. Kiss. 4.1 Phototropism: General Description and Distribution. 4.2 Light Perception. 4.3 Signal Transduction and Growth Response. 4.4 Interactions with Gravitropism. 4.5 Importance to Plant Form and Function. 4.6 Conclusions and outlook. 4.7 References. 5. Touch Sensing and Thigmotropism: Gabriele B. Monshausen, Sarah J. Swanson and Simon Gilroy. 5.1 Introduction. 5.2 Plant mechanoresponses. 5.2.1 Specialized touch responses. 5.2.2 Thigmomorphogenesis and thigmotropism. 5.3 General principles of touch perception. 5.3.1 Gating through membrane tension: the mechanoreceptor for hypoosmotic stress in bacteria, MscL. 5.3.2 Gating through tethers: the mechanoreceptor for gentle touch in Caenorhabditis elegans. 5.3.3 Evidence for mechanically gated ion channels in plants. 5.4 Signal transduction in Touch & Gravity Perception. 5.4.1 Ionic signaling. 5.4.2 Ca2+ signaling in the touch and gravity response. 5.5 Insights from transcriptional profiling. 5.6 Interaction of touch and gravity signaling/response. 5.7 Conclusion and Perspectives. 5.8 Acknowledgements. 5.9 Cited Literature. 6. Other Tropisms and their Relationship to Gravitropism: Gladys I. Cassab. 6.1 Introduction. 6.2 Hydrotropism. 6.2.1 Early studies of hydrotoprism. 6.2.2 Genetic analysis of hydrotropism. 6.2.3 Perception of moisture gradients and gravity stimuli by the root cap and the curvature response. 6.2.4 ABA and the hydrotropic response. 6.2.5 Future experiments. 6.3 Electrotropism. 6.4 Chemotropism. 6.5 Thermotropism and oxytropism. 6.6 Traumatropism. 6.7 Overview. 6.8 Acknowledgments. 6.9 Literature cited. 7. Single-Cell Gravitropism and Gravitaxis: Markus Braun and Ruth Hemmersbach. Introduction. 7.1 Definitions of responses to environmental stimuli that optimize the ecological fitness of single-cell organisms. 7.2 Occurrence and significance of gravitaxis in single-cell systems. 7.3 Significance of gravitropism in single-cell systems. 7.4 What makes a cell a biological gravity sensor?. 7.5 Gravity susception - the initial physical step of gravity sensing. 7.6 Susception in the statolith-based systems of Chara. 7.7 Susception in the statolith-based system Loxodes. 7.8 Susception in the protoplast-based systems of Euglena and Paramecium. 7.9 Graviperception in the statolith-based systems of Chara. 7.10 Graviperception in the statolith-based system Loxodes. 7.11 Graviperception in the protoplast-based systems Paramecium and Euglena. 7.12 Signal transduction pathways and graviresponse mechanisms in the statolith-based systems of Chara. 7.13 Signal transduction pathways and graviresponse mechanisms in Euglena and Paramecium. 7.14 Conclusions. 7.15 Acknowledgements. 7.18 Cited Literature. 8. Space-Based Research on Plant Tropisms: Melanie J. Correll and John Z. Kiss. 8.1 Introduction - the variety of plant movements. 8.2 The microgravity environment. 8.3 Ground-based studies: mitigating the effects of gravity. 8.4 Gravitropism. 8.4.1 Gravitropism: gravity perception. 8.4.2 Gravitropism: signal transduction. 8.4.3 Gravitropism: the curving response. 8.5 Phototropism. 8.6 Hydrotropism, autotropism and oxytropism. 8.7 Studies of other plant movements in microgravity. 8.8 Spaceflight hardware used to study tropisms. 8.9 Future outlook and prospects. 8.10 Cited Literature. . 9. Plan(t)s for Space Exploration: Christopher S. Brown, Heike Winter Sederoff, Eric Davies, Robert J. Ferl, and Bratislav Stankovic. Introduction. 9.1 Human missions to space. 9.2 Life support. 9.3 Genomics and space exploration. 9.4 Nanotechnology. 9.5 Sensors, biosensors and intelligent machines. 9.6 Plan(t)s for space exploration. 9.7 Imagine…. 9.8 Literature cited
£223.16
Springer-Verlag New York Inc. Pericytes
Book SynopsisTable of ContentsPreface…Table of Contents…Contributing Authors…1. Visualizing Pericyte Mimicry of Angiotropic Melanoma by Direct Labeling of the Angioarchitecture Roshini Prakash, Nikita Shivani Thareja, Thomas S. Carmichael, Raymond L. Barnhill, Claire Lugassy, and Laurent A. Bentolila2. Transplantation of Purified Human Pericytes for Myocardial Repair in SCID-hu MiceJames E. Baily and William C.W. Chen3. Assessment of Pericyte Phenotype by Flow CytometryN. S. Khan, C.C. West, F. Rossi, and M. Crisan4. Characterization of Pericyte Phenotype In Vivo by ImmunohistochemistryC.C. West, N.S. Khan, and M. Crisan5. A Metabolomics Based Approach to Identify Lineage Guiding Molecules in Pericyte Cultures E.V. Alakpa, C.C. West, L. Goldie, M. Harper, K. Burgess, R. Ulijn, and M.J. Dalby6. Pericyte Ontogeny: The Use of Chimeras to Track a Cell Lineage of Diverse Germ Line OriginsHeather C. Etchevers7. Imaging Pericytes and the Regulation of Cerebral Blood FlowKatie Boyd, Matthew Hammond-Haley, Rozan Vroman, and Catherine Hall8. Derivation of Pericytes from Human Pluripotent Stem CellsAyelet Dar and Joseph Itskovitz-Eldor9. Assessing the Bone-Forming Potential of Pericytes Carolyn A. Meyers, Chenchao Wang, Jiajia Xu, Hsin Chuan Pan, Jia Shen, Kang Ting, Chia Soo, Bruno Peault, and Aaron W. James10. Manipulating Pericyte Function with MicroRNAsMilena Vitiello, Ben Cathcart, Andrea Caporali, and Marco Meloni11. Isolation of Mesoangioblasts: A Subset of Pericytes with Strong Myogenic Potential from Mammals Giorgia Giacomazzi, Gaia Giovannelli, Alessio Rotini, Domiziana Costamagna, Mattia Quattrocelli, and Maurilio Sampaolesi12. Role of Pericytes in the Development of the Renin/Angiotensin System: Induction of Functional Renin in Cultures of Pericytes Ania Stefanska, Angela Briski, Nusrat Khan, Christopher Kenyon, John J. Mullins, and Bruno Péault13. Metastatic Dissemination Mimicked in a Multicellular Transwell AssayCourtney König and Anja RungeSubject Index List…
£98.99
Springer-Verlag New York Inc. Craniofacial Development
Book SynopsisThis volume explores scientific methodologies currently employed to integrate observational developmental biology, tissue explant and cell-based approaches and genetic/molecular technologies to develop a holistic understanding of craniofacial development. Chapters guide readers through the use of disparate models to study formation of the head and face (c. elegans, zebrafish, mouse, alongside human imaging approaches), together with cell culture, tissue explant and in vivo cell imaging and analysis techniques. At the molecular level, chapters include analysing gene expression using in-situ hybridisation and single-cell RNA-Sequencing (scRNA-SEQ), as well as genetic modification techniques such as CRISPR/Cas9-mediated deletion. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tiTable of Contents1. Using Caenorhabditis elegans as a model for mechanistic insights of craniofacial development Michael Gruss and Ann K. Corsi 2. Wholemount In-situ Hybridisation (WISH) in Zebrafish Embryos to Analyse Craniofacial DevelopmentNishanthi Mathiyalagan and Sebastian Dworkin 3. Elucidation of Gene Expression Patterns in The Craniofacial Tissues Of Mouse Embryos By Whole-Mount In Situ Hybridization Joshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam 4. Visualization of the Cartilage and Bone Elements in The Craniofacial Structures by Alcian blue and Alizarin Red Staining Joshua B Studdert, Heidi Bildsoe, V Pragathi Masamsetti, and Patrick P L Tam 5. Methodology for Free-Floating Organ Culture of Mid-Gestation Maxillary Primordial Tissue M. E. de Vries and S. Dworkin 6. Methods of Palate Culture in Later Palatogenesis: Elevation, Horizontal Outgrowth, and Fusion Jack D. Morgan and Jeremy B. A. Green 7. Single cell RNA-seq: Cell Isolation and Data Analysis Val Yianni and Paul T Sharpe 8. Generating Zebrafish RNA-less Mutant Alleles by Deleting Gene Promoters with CRISPR/Cas9 Priyanka Kumari, Morgan Sturgeon, Gregory Bonde, and Robert A. Cornell 9. Craniofacial Phenomics: Three-Dimensional Assessment of the Size and Shape of Cranial and Dentofacial Structures Mustafa Mian, Jenny Tan, Robin Yong, Ruth Williams, Agatha Labrinidis, Peter J. Anderson, and Sarbin Ranjitkar 10. Micro-CT-based Bone Microarchitecture Analysis of the Murine Skull Jenny Tan, Agatha Labrinidis, Ruth Williams, Mustafa Mian, Peter J. Anderson, and Sarbin Ranjitkar 11. Characterization of mammalian in vivo enhancers using mouse transgenesis and CRISPR genome editing Marco Osterwalder, Stella Tran, Riana D. Hunter, Eman M. Meky, Kianna von Maydell, Anne N. Harrington, Janeth Godoy, Catherine S. Novak, Ingrid Plajzer-Frick, Yiwen Zhu, Jennifer A. Akiyama, Veena Afzal, Evgeny Z. Kvon, Len A. Pennacchio, Diane E. Dickel, and Axel Visel 12. Histological Techniques for Sectioning Bones of the Vertebrate Craniofacial Skeleton Daniela M. Roth, Lakshmi Puttagunta, and Daniel Graf 13. Engineering Epithelial-Mesenchymal Microtissues to Study Cell-Cell Interactions in Development Jacob I. Reynolds, Ross A. Vitek, Peter G. Geiger, and Brian P. Johnson 14. Ex Vivo Culture of Human Cranial Suture Cells Peter J. Anderson 15. Scaffolds for Use in Craniofacial Bone Regeneration Katherine R. Hixon, Christopher T. Eberlin, Meghana Pendyala, Angela Alarcon de la Lastra, and Scott A. Sell 16. Mandible Explant Assay for The Analysis of Meckel’s Cartilage Development Sophie Wiszniak and Quenten Schwarz 17. High Resolution Histology for Craniofacial Studies On Zebrafish and Other Teleost Models A. Huysseune, M. Soenens, J.-Y. Sire, and P.E. Witten 18. Live Imaging the Dynamics Of Mammalian Neural Crest Cell Migration Emma L. Moore and Paul A. Trainor 19. Salivary Gland Development In Culture Marcia Gaete, Tathyane H. N. Teshima, Lemonia Chatzeli, and Abigail S. Tucker 20. Antenatal Ultrasound Imaging for Analysis of Human Craniosynostosis Sarah Constantine and Piotr Niznik
£161.99
Springer-Verlag New York Inc. Plasmodesmata
Book SynopsisThis fully updated book aims to facilitate the study of the nanochannels that connect plant cells, known as plasmodesmata, and to instigate new research that will further advance our knowledge of these structures. Beginning with the general structural composition and regulation of plasmodesmata as well as their role in plant development and disease, the volume continues with chapters exploring plasmodesmata architectures and distribution in cell interfaces, approaches to dissect plasmodesmata composition, protocols to quantify changes in plasmodesmata permeability using fluorescent tracers and mobile proteins, as well as a section with protocols that contribute to plasmodesmata research but fall outside the previous classifications. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooTable of ContentsPart I: Overview Articles 1. Plasmodesmata Structural Components and Their Role in Signaling and Plant Development Philip Kirk and Yoselin Benitez-Alfonso 2. Function of Plasmodesmata in the Interaction of Plants with Microbes and Viruses Caiping Huang and Manfred Heinlein Part II: Structure and Distribution of Plasmodesmata 3. Plasmodesmata Ultrastructure Determination Using Electron Tomography Jules D. Petit, Marie Glavier, Lysiane Brocard, and Emmanuelle Bayer 4. Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata Kamila Godel-Jędrychowska, Tilman Franke, and Ewa Kurczyńska 5. Serial Block Electron Microscopy to Study Plasmodesmata in the Vasculature of Arabidopsis thaliana Roots Andrea Paterlini and Ilya Belevich 6. Focused Ion Beam-Scanning Electron Microscopy for Investigating Plasmodesmal Densities Brandon C. Reagan, John R. Dunlap, and Tessa M. Burch-Smith 7. Measuring Plasmodesmata Density on Cell Interfaces of Monocot Leaves Using 3D Immunolocalization and Scanning Electron Microscopy Florence R. Danila 8. Super-Resolution Imaging of Plasmodesmata Using 3D-Structured Illumination Microscopy Kirsten Knox Part III: Compositional Analysis of Plasmodesmata 9. In Vivo Aniline Blue Staining and Semi-Automated Quantification of Callose Deposition at Plasmodesmata Caiping Huang, Jerôme Mutterer, and Manfred Heinlein 10. Immunofluorescence Detection of Callose in Plant Tissue Sections Sam Amsbury and Yoselin Benitez-Alfonso 11. Callose Detection and Quantification at Plasmodesmata in Bryophytes Arthur Muller, Tomomichi Fujita, and Yoan Coudert 12. Isolation of Plasmodesmata Membranes for Lipidomic and Proteomic Analysis Laetitia Fouillen, Stéphane Claverol, Emmanuelle M.F. Bayer, and Magali S. Grison 13. Methods for Detection of Protein Interactions with Plasmodesmata-Localized Reticulons Verena Kriechbaumer and Stanley W. Botchway 14. Studying Protein-Protein Interactions at Plasmodesmata by Measuring Förster Resonance Energy Transfer Patrick Blümke, Vicky Howe, and Rüdiger Simon 15. Quantifying the Organization and Dynamics of the Plant Plasma-Membrane across Scales Using Light Microscopy Joseph F. McKenna 16. Using Steady-State Fluorescence Anisotropy to Study Protein Clustering Ajeet Chaudhary and Kay Schneitz Part IV: Functional Analysis of Plasmodesmata 17. Quantification of Cell-to-Cell Connectivity Using Particle Bombardment Estee E. Tee, Sebastian Samwald, and Christine Faulkner 18. Investigating Plasmodesmata Function in Arabidopsis thaliana Using a Low-Pressure Bombardment System and GFP Movement Assay Jessica C. Fernandez and Tessa M. Burch-Smith 19. Quantifying Plasmodesmatal Transport with an Improved GFP Movement Assay Wilson Horner and Jacob O. Brunkard 20. An Arabidopsis Callus Grafting Method to Test Cell-to-Cell Mobility of Proteins Frank Machin, Yağmur Hasbioğlu, and Friedrich Kragler 21. Quantification of Plasmodesmata Permeability in Arabidopsis Leaves by Tracing the Movement of GFP Min Diao and Shanjin Huang 22. Tracking Intercellular Movement of Fluorescent Proteins in Bryophytes Takumi Tomoi, Yoan Coudert, and Tomomichi Fujita 23. Virus Genome-Based Reporter for Analyzing Viral Movement Proteins and Plasmodesmata Permeability Ekaterina A. Lazareva, Anastasia K. Atabekova, Alexander A. Lezzhov, Sergey Y. Morozov, Manfred Heinlein, and Andrey G. Solovyev 24. Analysis of the Distribution of Symplasmic Tracers during Zygotic and Somatic Embryogenesis Justyna Wróbel-Marek, Kamila Godel-Jędrychowska, and Ewa Kurczyńska 25. Quantifying Intercellular Movement and Protein Stoichiometry for Computational Modeling Lisa Van den Broeck, Mariah Gobble, and Rosangela Sozzani 26. Spatiotemporal Specific Blocking of Plasmodesmata by Callose Induction Dawei Yan 27. A Forward Genetic Approach to Identify Plasmodesmal Trafficking Regulators Based on Trichome Rescue Munenori Kitagawa and David Jackson Part V: Other Approaches Contributing to Plasmodesmata Research 28. In Vivo Visualization of Mobile mRNA Particles in Plants Using BglG Eduardo J. Peña and Manfred Heinlein 29. Multi-Angle In Vivo Imaging of the Arabidopsis thaliana Shoot Apical Meristem (SAM) Michael Fuchs and Jan U. Lohmann 30. More Insights from Ultrastructural and Functional Plasmodesmata Data Using PDinsight Eva E. Deinum 31. Measuring Intercellular Interface Area in Plant Tissues Using Quantitative 3D Image Analysis Gwendolyn V. Davis, Richard S. Smith, and George W. Bassel
£170.99
Springer-Verlag New York Inc. cAMP Signaling
Book SynopsisThis volume discusses the latest techniques used by researchers to measure cAMP activity at the cell population, whole cell, and subcellular level. The techniques covered in the book address questions related to cAMP compartmentalization, which look at relevant protein-protein interactions that increase the spatial and temporal resolution of cAMP signals detection, and that can help in the integration of the increasingly complex information that is becoming available in this field. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, cAMP Signaling: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers who are interested in learTable of ContentsPreface…Table of Contents...Contributing Authors…1. Real-Time Measurements of Intracellular cAMP Gradients using FRET-Based cAMP NanorulersCharlotte Konrad, Martin J. Lohse, and Andreas Bock2. Assaying Protein Kinase A Activity using a FRET-Based Sensor Purified from Mammalian CellsAshton J. Curtis, Ryan S. Dowsell, and Matthew G. Gold3. MultiFRET: A Detailed Protocol for High-Throughput Multiplexed Ratiometric FRETMasoud Ramuz, Ivan Diakonov, Chris Dunsby, and Julia Gorelik4. Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal ActivityThilo Henss, Martin Schneider, Dennis Vettkötter, Wagner Steuer Costa, Jana F. Liewald, and Alexander Gottschalk5. Imaging the cAMP Signaling Microdomain of the Primary Cilium using Targeted FRET-Based BiosensorsDanielle T. Arena and Aldebaran M. Hofer6. Methods to Assess Phosphodiesterase and/or Adenylyl Cyclase Activity via Heterologous Expression in Fission YeastMarek Domin and Charles S. Hoffman7. Time-Domain Fluorescence Lifetime Imaging of cAMP Levels with EPAC-Based FRET SensorsOlga Kukk, Jeffrey Klarenbeek, and Kees Jalink8. Disruptors of AKAP-Dependent Protein-Protein InteractionsRyan Walker-Gray, Tamara Pallien, Duncan C. Miller, Andreas Oder, Martin Neuenschwander, Jens Peter von Kries, Sebastian Diecke, and Enno Klussmann9. Micro-2D Cell Culture for cAMP Measurements using FRET Reporters in Human iPSC-Derived Cardiomyocytes Andreas Koschinski and Manuela Zaccolo10. Automated Image Analysis of FRET Signals for Subcellular cAMP QuantificationSilas J. Leavesley, Naga Annamdevula, Santina Johnson, DJ Pleshinger, and Thomas C. Rich11. In Vivo cAMP Dynamics in Drosophila Larval NeuronsIsabella Maiellaro12. Live Cell Imaging of Cyclic Nucleotides in Human CardiomyocytesKira Beneke and Cristina E. Molina13. Optogenetic Control of Heart Rhythm: Lightly Guiding the Cardiac PaceLolita Dokshokova, Nicola Pianca, Tania Zaglia, and Marco Mongillo14. Live Imaging of cAMP Signaling in D. discoideum Based on a Bright Bioluminescent Protein, Nano-LanternKazuki Horikawa and Takeharu Nagai15. Generation of Transgenic Mice Expressing Cytosolic and Targeted FRET Biosensors for cAMP and cGMPRoberta Kurelić and Viacheslav O. Nikolaev16. How to Make the CUTiest Sensor in 3 Simple Steps for Computational PedestriansFlorencia Klein, Cecilia Abreu, and Sergio Pantano17. Ion-Channel Based Reporters for cAMP DetectionThomas C. Rich, Wenkuan Xin, Silas J. Leavesley, C. Michael Francis, and Mark Taylor18. Quantitative Phosphoproteomics to Study cAMP SignallingKatharina Schleicher, Svenja Hester, Monika Stegmann, and Manuela Zaccolo 19. Biochemical Analysis of AKAP-Anchored PKA Signaling ComplexesDominic P. Byrne, Mitchell H. Omar, Eileen J. Kennedy, Patrick A. Eyers, and John D. Scott20. Fluorescent Translocation Reporters for Sub-Plasma Membrane cAMP ImagingOleg Dyachok, Yunjian Xu, Olof Idevall-Hagren, and Anders Tengholm21. A Live-Cell Imaging Assay for Nuclear Entry of cAMP-Dependent Protein Kinase Catalytic Subunits Stimulated by Endogenous GPCR Activation Grace E. Peng and Mark von Zastrow22. Measuring Spatiotemporal cAMP Dynamics within an Endogenous Signaling Compartment using FluoSTEP-ICUEJulia C. Hardy, Sohum Mehta, and Jin ZhangSubject Index List…
£143.99
Springer-Verlag New York Inc. TGFBeta Signaling
Book SynopsisThis detailed volume is devoted to the recent development of quantitative experiments and computational methods driving new transforming growth factor beta (TGF-ß) and other cell signaling knowledge.Table of Contents1. Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot Difan Deng and Zhike Zi 2. Fast Quantitation of TGF-β Signaling Using Adenoviral Reporter Adilson Fonseca Teixeira, Josie Iaria, and Hong-Jian Zhu 3. Complex Formation among TGF-b Receptors in Live Cell Membranes Measured by Patch-FRAP Szabina Szófia Szilágyi, Orit Gutman, and Yoav I. Henis 4. Branched Proximity Hybridization Assay for the Quantification of Nanoscale Protein-Protein Proximity Jianying Yang 5. Visualizing Dynamic Changes during TGF-β-Induced Epithelial to Mesenchymal Transition Abhishek Sinha, Pranav Mehta, Chuannan Fan, Jing Zhang, Dieuwke L. Marvin, Maarten van Dinther, Laila Ritsma, Pouyan E. Boukany, and Peter ten Dijke 6. Establishment of Embryonic Zebrafish Xenograft Assays to Investigate TGF-β Family Signaling in Human Breast Cancer Progression Chao Li, Jin Ma, Arwin Groenewoud, Jiang Ren, Sijia Liu, B. Ewa Snaar-Jagalska, and Peter ten Dijke 7. Generating Somatic Knockout Cell Lines with CRISPR-Cas9 Technology to Investigate SMAD Signaling Zixin Huang and Alexander Loewer 8. CRISPR-Based Screening in Three-Dimensional Organoid Cultures to Identify TGF-β Pathway Regulators Nina Frey and Gerald Schwank 9. Optogenetic Control of TGF-β Signaling Yuchao Li and Zhike Zi 10. Using Microfluidics and Live Cell Reporters to Dissect the Dynamics of TGF-β Signaling in Mouse Embryonic Stem Cells Fabien Furfaro, Carine Vias, and Benoit Sorre 11. Energy Landscape Analysis of the Epithelial-Mesenchymal Transition Network Leijun Ye and Chunhe Li 12. Discrete Logic Modeling of Cell Signaling Pathways Nensi Ikonomi, Silke D. Werle, Julian D. Schwab, and Hans A. Kestler 13. Mining of Single-Cell Signaling Time-Series for Dynamic Phenotypes with Clustering Maciej Dobrzyński, Marc-Antoine Jacques, and Olivier Pertz 14. Automated Classification of Cellular Phenotypes Using Machine Learning in Cellprofiler and CellProfiler Analyst Marja Kornhuber and Sebastian Dunst 15. Live Cell Imaging of Spatiotemporal Ca2+ Fluctuation Responses to Anticancer Drugs Zeyu Liu, Adrian Ramirez, and Xuedong Liu
£151.99
Springer-Verlag New York Inc. Celebrating J.D. Murrays Contributions to
Book SynopsisThe Bulletin of Mathematical Biology, the official journal of the Society for Mathematical Biology, disseminates original research findings and other information relevant to the interface of biology and the mathematical sciences. Contributions should have relevance to both fields. In order to accommodate the broad scope of new developments, the journal accepts a variety of contributions, including: Original research articles focused on new biological insights gained with the help of tools from the mathematical sciences or new mathematical tools and methods with demonstrated applicability to biological investigations Research in mathematical biology education Reviews Commentaries Perspectives, and contributions that discuss issues important to the profession All contributions are peer-reviewed.
£67.49
Springer-Verlag New York Inc. The Plant Cytoskeleton
Book SynopsisThis detailed volume explores the development of technologies and protocols that are currently being used to understand the nature and activities of the plant cytoskeleton. A focus for many of the chapters is on sample preparation, as the quality of plant organ/tissue preparation, from single to multicellular samples, determines the quality of the data. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Plant Cytoskeleton: Methods and Protocols serves as an ideal guide for researchers interested in or starting to be interested in plant cell and molecular biology research.Table of Contents1. Quantification of Microtubule Bundling Activity of MAPs Using TIRF Microscopy Sharol Schmidt-Marcec, Austin Ross, and Andrei Smertenko 2. Actin: Static and Dynamic Studies Huaqiang Ruan, Sha Zhang, Yi Zhang, and Haiyun Ren 3. 3D Visualization of Microtubules in Epidermal Pavement Cells Amir J. Bidhendi, Bara Altartouri, and Anja Geitmann 4. Quantitative Analysis of Microtubule Organization in Leaf Epidermis Pavement Cells Sandra Klemm, Jonas Buhl, Birgit Möller, and Katharina Bürstenbinder 5. Single Cell Confinement Methods to Study Plant Cytoskeleton Pauline Durand-Smet, Antoine Chevallier, Léia Colin, Alice Malivert, Isaty Melogno, and Olivier Hamant 6. Documentation of Microtubule Collisions with Myosin VIII ATM1 Containing Membrane-Associated Structures Eduard Belausov, Vikas Dwivedi, Sela Yechezkel, Sefi Bar-Sinai, and Einat Sadot 7. Imaging the Plant Cytoskeleton by High-Pressure Freezing and Electron Tomography Janice Pennington and Marisa S. Otegui 8. Confocal Microscopic Assays of Mitotically Active Proteins in an Agrobacterial Infiltration-Based, Cell Division-Enabled Leaf System of Tobacco (Nicotiana benthamiana) Yuh-Ru Julie Lee, Calvin H. Huang, and Bo Liu 9. Assessment of Spindle Shape Control by Spindle Poleward Flux Measurements and FRAP Bulk Analysis Sabine Müller 10. Expansion Microscopy of Plant Cells (PlantExM) Timothy J. Hawkins, Joanne L. Robson, Bethany Cole, and Simon J. Bush 11. Microfluidic Device for High-Resolution Cytoskeleton Imaging and Wash Out Assays in Physcomitrium (Physcomitrella) patens Mari W. Yoshida and Elena Kozgunova 12. Using Spinning Disk Microscopy to Observe the Mitotic and Cytokinetic Apparatus in Physcomitrium patens Yuji Hiwatashi and Takashi Murata 13. Gaining Insight into Large Gene Families with the Aid of Bioinformatic Tools Fatima Cvrčková and Radek Bezvoda 14. Cell-to-Cell Connectivity Assays for the Analysis of Cytoskeletal and Other Regulators of Plasmodesmata Zoe Barr and Jens Tilsner 15. Studying Nuclear Dynamics in Response to Actin Disruption In Planta Joseph F. McKenna and Katja Graumann 16. Cytoskeleton Remodelling in Arabidopsis Stigmatic Cells Following Pollination Lucie Riglet and Isabelle Fobis-Loisy 17. Investigation of ROP GTPase Activity and Cytoskeleton Dynamics during Tip Growth in Root Hairs and Pollen Tubes Lei Zhu and Ying Fu 18. Functional Analysis of Phospholipid Signaling and Actin Dynamics: The Use of Apical Growing Tobacco Pollen Tubes in a Case Study Teresa Braga, Fernando Vaz Dias, Marta Fratini, Susana Serrazina, Ingo Heilmann, and Rui Malhó 19. Microtubule Reorganization during ABA-Induced Stomatal Closure in Arabidopsis Liru Dou, Xiangfeng Wang, and Tonglin Mao 20. Imaging of Cortical Microtubules in Plants under Salt Stress Shuwei Wang, Liyuan Xu, Changjiang Li, Lei Zhu, Ying Fu, and Yan Guo 21. Analysis of Actin Array Rearrangement during the Plant Response to Bacterial Stimuli Bingxiao Wang, Minxia Zou, Qing Pan, and Jiejie Li 22. Live-Cell Imaging of Cytoskeletal Responses and Trafficking during Fungal Elicitation Amber J. Connerton, Stefan Sassmann, and Michael J. Deeks 23. Visualization and Quantification of the Dynamics of Actin Filaments in Arabidopsis Pollen Tubes Qiaonan Lu, Xiaonan Liu, Xiaolu Qu, and Shanjin Huang 24. Non-Invasive Long-Term Imaging of the Cytoskeleton in Arabidopsis Seedlings Felix Ruhnow, Staffan Persson, and René Schneider 25. Visualization of Cytoskeleton Organization and Dynamics in Elongating Cotton Fibers by Live-Cell Imaging Guangda Wang, Yanjun Yu, and Zhaosheng Kong 26. Methods to Visualize and Quantify Cortical Microtubule Arrays in Arabidopsis Conical Cells Xie Dang, Lilan Zhu, Huibo Ren, and Deshu Lin 27. Studying the Organization of the Actin Cytoskeleton in the Multicellular Trichomes of Tomato Zhijing Xu, Xiaolu Qu, Shuang Wu, and Pengwei Wang 28. Light Microscopy Technologies and the Plant Cytoskeleton Timothy J. Hawkins 29. Investigating Plant Protein-Protein Interactions Using FRET-FLIM with a Focus on the Actin Cytoskeleton Patrick Duckney and Patrick J. Hussey
£179.99
Springer-Verlag New York Inc. Cell Migration in Three Dimensions
Book SynopsisThis detailed collection serves as a unique and excellent collection of state-of-the-art methods and protocols to interrogate cell migration in a wide variety of different contexts and model organisms, as well as advanced image analysis and quantitative assessment of a diverse array of parameters related to cell migration.Table of Contents1. Cell Migration in Three Dimensions Coert Margadant Part I: The Cell Biology of Cell Migration 2. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, and David A. Calderwood 3. Probing the ER-Focal Adhesion Link during Cell Migration Noemi Antonella Guadagno and Cinzia Progida 4. Mapping the Localization of Proteins within Filopodia Using FiloMap Guillaume Jacquemet 5. Extended Methods for 2D Confinement Juan Manuel García-Arcos, Kevin Gateau, Larisa Venkova, and Matthieu Piel 6. Visualization of Exosome Release and Uptake during Cell Migration Using the Live Imaging Reporter pHluorin_M153R-CD63 Bong Hwan Sung and Alissa M. Weaver 7. Approaches to Determine Nuclear Shape in Cells during Migration through Collagen Matrices Martin Svoren, Elena Camerini, Merijn van Erp, Feng Wei Yang, Gert-Jan Bakker, and Katarina Wolf Part II: Developmental Model Systems to Assess Cell Migration during Morphogenesis 8. Dissecting Collective Cell Behavior in Migrating Testis Myotubes in Drosophila Maik C. Bischoff and Sven Bogdan 9. Whole Mount In Situ Hybridization for Detection of Migrating Zebrafish Endodermal Cells Antonius L. van Boxtel 10. Live Imaging Analysis of Epithelial Zippering during Mouse Neural Tube Closure Matteo A. Molè, Gabriel L. Galea, and Andrew J. Copp 11. Time-Lapse Imaging and Morphometric Analysis of Tracheal Development in Drosophila Sofia J. Araújo and Marta Llimargas 12. A Guide Toward Multi-Scale and Quantitative Branching Analysis in the Mammary Gland Edouard Hannezo and Colinda L.G.J. Scheele 13. Analysis of Integrin-Dependent Melanoblast Migration during Development Amanda Haage and Guy Tanentzapf Part III: Cell Migration in Cancers and the Tumor Microenvironment 14. Invadopodia Methods: Detection of Invadopodia Formation and Activity in Cancer Cells Using Reconstituted 2D and 3D Collagen-Based Matrices David Remy, Anne-Sophie Macé, Philippe Chavrier, and Pedro Monteiro 15. Analysis of Energy-Driven Leader-Follower Hierarchy during Collective Cancer Cell Invasion Jian Zhang and Cynthia A. Reinhart-King 16. Visualizing and Quantifying mRNA Localization at the Invasive Front of 3D Cancer Spheroids Konstadinos Moissoglu, Stephen J. Lockett, and Stavroula Mili 17. Multimodal Techniques to Study Tumor Growth, Basement Membrane Breaching, and Invasion in 3D Matrices Daan Smits and Antoine A. Khalil 18. Analysis of Collective Migration Patterns within Tumors Ralitza Staneva and Andrew G. Clark 19. An In Vivo Model to Study Cell Migration in XYZ-T Dimension Followed by Whole-Mount Re-Evaluation Ann L.B. Seynhaeve and Timo L.M. ten Hagen Part IV: Blood Vessel Formation and Interactions 20. Angiogenesis Invasion Assay to Study Endothelial Cell Invasion and Sprouting Behavior Yuechao Dong, Florian Alonso, Tiya Jahjah, Isabelle Fremaux, and Elisabeth Génot 21. Live-Cell Labeling and Artificial Intelligence Approaches for High-Resolution XYZT Imaging of Cytoskeletal Dynamics during Collective Cell Migration Maxime Cammeraat, Marko Popovic, Wendy Stam, and Coert Margadant 22. Analysis of mRNA Subcellular Distribution in Collective Cell Migration Joshua J. Bradbury, Holly E. Lovegrove, Marta Giralt-Pujol, and Shane P. Herbert 23. A Bio-Engineered Model for Studying Vascular-Pericyte Interactions of the Placenta Marta Cherubini and Kristina Haase 24. Analysis of Vascular Morphogenesis in Zebrafish Marvin Hubert and Wiebke Herzog 25. Analysis of Monocyte Recruitment during Inflammation by Intravital Imaging Jose M. Gonzalez-Granado, Alberto Del Monte, Laura Piqueras, Vicente Andres, and Cristina Rius
£107.99
Springer-Verlag New York Inc. Liposomes
Book SynopsisThis third edition provides new and updated chapters detailing preparation of liposomes, physicochemical characterization of liposomes, lipid analysis, drug encapsulation, surface modification, stimuli response as well as cellular interaction, and biodistribution. Also included is an updated chapter on the history and evolution of the field of liposomology. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Liposomes: Methods and Protocols, Third Edition aims to serve as a reference for graduate students, post-doctoral researchers as well as established investigators utilizing lipid-based systems.Table of Contents1. From olive oil emulsions to COVID-19 Vaccines - Liposomes Came First Volkmar Weissig 2 Preparation of DRV liposomes Sophia G. Antimisiaris 3 Preparation of small unilamellar vesicles using detergent dialysis method Qingyue Zhong and Hongwei Zhang 4 Thin-film hydration followed by extrusion method for liposome preparation Hongwei Zhang 5 Ethanol injection method for liposome preparation Guangsheng Du and Xun Sun 6 Preparation of giant vesicles with mixed single-tail and double-tail lipids Lauren A Lowe and Anna Wang 7 Scalable Liposome Synthesis by High Aspect Ratio Microfluidic Flow Focusing Jung Yeon Han, Zhu Chen, and Don L. DeVoe 8 Preparation of doxorubicin liposomes by remote loading method Jian Chen 9 Magnetic thermosensitive liposomes loaded with Doxorubicin Mohamad Alawak, Alice Abu Dayyih, Ibrahim Awak, Bernd Gutberlet, Konrad Engelhardt, and Udo Bakowsky 10 Preparation and physical characterization of DNA binding cationic liposomes Vaibhav Saxena 11 Tunable pH sensitive lipoplexes Helene Dhotel, Michel Bessodes and Nathalie Mignet 12 Solid Lipid Nanoparticles for Drug Delivery Wei-Chung Luo and Xiuling Lu 13 Stable Discoidal Bicelles – Formulation, Characterization and Functions Ying Liu, Yan Xia, Armin Tahmasbi Rad, Wafa Aresh, Justin M. Fang, and Mu-Ping Nieh 14 The post-insertion method for the preparation of PEGylated liposomes Sherif E. Emam, Nehal E. Elsadek, Taro Shimizua, and Tatsuhiro Ishidaa 15 Click chemistry for liposome surface modification Maria Vittoria Spanedda, Marcella De Giorgi, Béatrice Heurtault, Antoine Kichler, Line Bourel-Bonnet and Benoît Frisch 16 Surface Modification of Liposomes Using Folic Acid Mengran Guo, Zhongshan He, Xi He and Xiangrong Song 17 Preparation and Characterization of Trastuzumab Fab conjugated liposomes (immunoliposomes) Yuhong Xu 18 Pyrophosphorylated cholesterol modified bone-targeting liposomes formulation procedure Yanzhi Liu, Zhenshan Jia, Luoyang Ma, and Dong Wang 19 Method of simultaneous analysis of liposome components using HPTLC/FID Sophia Hatziantoniou and Costas Demetzos 20 HPLC-MS/MS Method for Identification and Quantification of Lipids in Liposomes Yujie Shi and Xiaona Li 21 DPH probe method for liposome-membrane fluidity determination Wei He 22 Imaging of Liposomes by Negative Staining Transmission Electron Microscopy and Cryogenic Transmission Electron Microscopy Anand S. Ubhe 23 Visualization and characterization of liposomes by Atomic Force Microscopy (AFM) Konrad Engelhardt, Eduard Preis and Udo Bakowsky 24 Determination of the sub-cellular distribution of fluorescently labeled liposomes using confocal microscopy Melani A. Solomon 25 Liposome biodistribution via europium complexes Nathalie Mignet and Daniel Scherman 26 Quantification of a fluorescent lipid DOPE-NBD by an HPLC method in biological tissue: application to study liposome’s uptake by human placenta Louise Fliedel, Nathalie Mignet, Thierry Fournier, Karine Andrieux, and Khair Alhareth
£179.99
