Biochemistry Books
John Wiley & Sons Inc Instrumental Analysis of Intrinsically Disordered
Book SynopsisInstrumental techniques for analyzing intrinsically disordered proteins The recently recognized phenomenon of protein intrinsic disorder is gaining significant interest among researchers, especially as the number of proteins and protein domains that have been shown to be intrinsically disordered rapidly grows. The first reference to tackle this little-documented area, Instrumental Analysis of Intrinsically Disordered Proteins: Assessing Structure and Conformation provides researchers with a much-needed, comprehensive summary of recent achievements in the methods for structural characterization of intrinsically disordered proteins (IDPs). Chapters discuss: Assessment of IDPs in the living cell Spectroscopic techniques for the analysis of IDPs, including NMR and EPR spectroscopies, FTIR, circular dichroism, fluorescence spectroscopy, vibrational methods, and single-molecule analysis Single-molecule techniques aTrade Review"With contributions from an international selection of leading researchers, this book fills an important need in a rapidly growing field. It is required reading for biochemists, biophysicists, molecular biologists, geneticists, cell biologists, physiologists, and specialists in drug design and development, proteomics, and molecular medicine with an interest in proteins and peptides." (Anticancer Research, 1 August 2010)Table of ContentsPREFACE. INTRODUCTION TO THE WILEY SERIES ON PROTEIN AND PEPTIDE SCIENCE. LIST OF CONTRIBUTORS. LIST OF ABBREVIATIONS. PART I ASSESSING IDPs IN THE LIVING CELL. 1 IDPs and Protein Degradation in The Cell (Yosef Shaul, Peter Tsvetkov, and Nina Reuven). 2 The Structural Biology of IDPs Inside Cells (Philipp Selenko). PART II SPECTROSCOPIC TECHNIQUES. 3 Nuclear Magnetic Resonance Spectroscopy Applied to (Intrinsically) Disordered Proteins (Frans A. A. Mulder, Martin Lundqvist, and Ruud M. Scheek). 4 Atomic-Level Characterization of Disordered Protein Ensembles Using NMR Residual Dipolar Couplings (Martin Blackledge, Pau Bernadó, and Malene Ringkjøbing Jensen). 5 Determining Structural Ensembles for Intrinsically Disordered Proteins (Gary W. Daughdrill). 6 Site-Directed Spin Labeling EPR Spectroscopy (Valérie Belle,, Sabrina Rouger, Stéphanie Costanzo, Sonia Longhi, and André Fournel). 7 The Structure of Unfolded Peptides and Proteins Explored by Vibrational Spectroscopy (Reinhard Schweitzer-Stenner, Thomas J. Measey, Andrew M. Hagarman, and Isabelle C. Dragomir). 8 Intrinsically Disordered Proteins and Induced Folding Studied by Fourier Transform Infrared Spectroscopy (Antonino Natalello and Silvia Maria Doglia). 9 Genetically Engineered Polypeptides as a Model of Intrinsically Disordered Fibrillogenic Proteins: Deep UV Resonance Raman Spectroscopic Study (Natalya I. Topilina, Vitali Sikirzhytski, Seiichiro Higashiya, Vladimir V. Ermolenkov, John T. Welch, and Igor K. Lednev). 10 Circular Dichroism of Intrinsically Disordered Proteins (Robert W. Woody). 11 Fluorescence Spectroscopy of Intrinsically Disordered Proteins (Eugene A. Permyakov and Vladimir N. Uversky). 12 Hydration of Intrinsically Disordered Proteins From Wide-Line NMR (Kálmán Tompa, Monika Bokor, and Peter Tompa). PART III SINGLE-MOLECULE TECHNIQUES. 13 Single-Molecule Spectroscopy of Unfolded Proteins (Benjamin Schuler). 14 Monitoring the Conformational Equilibria of Monomeric Intrinsically Disordered Proteins by Single-Molecule Force Spectroscopy (Massimo Sandal, Marco Brucale, and Bruno Samorì). PART IV METHODS TO ASSESS PROTEIN SIZE AND SHAPE. 15 Analytical Ultracentrifugation, a Useful Tool to Probe Intrinsically Disordered Proteins (Florence Manon and Christine Ebel). 16 Structural Insights into Intrinsically Disordered Proteins by Small-Angle X-Ray Scattering (Pau Bernadó and Dmitri I. Svergun). 17 Dynamic and Static Light Scattering (Klaus Gast). 18 Analyzing Intrinsically Disordered Proteins by Size Exclusion Chromatography (Vladimir N. Uversky). PART V CONFORMATIONAL STABILITY 545 19 Conformational Behavior of Intrinsically Disordered Proteins: Effects of Strong Denaturants, Temperature, PH, Counterions, and Macromolecular Crowding (Vladimir N. Uversky). 20 Detecting Disordered Regions in Proteins by Limited Proteolysis (Angelo Fontana, Patrizia Polverino de Laureto, Barbara Spolaore, Erica Frare, and Marcello Zambonin). PART VI MASS SPECTROMETRY. 21 Mass Spectrometry Tools for the Investigation of Structural Disorder and Conformational Transitions in Proteins (Mária Šamalíková, Carlo Santambrogio, and Rita Grandori). PART VII EXPRESSION AND PURIFICATION OF IDPS. 22 Recombinant Production of Intrinsically Disordered Proteins for Biophysical and Structural Characterization (Dmitri Tolkatchev, Josee Plamondon, Richard Gingras, Zhengding Su, and Feng Ni). 23 Large-Scale Identifi cation of Intrinsically Disordered Proteins (Vladimir N. Uversky, Marc S. Cortese, Peter Tompa, Veronika Csizmok, and A. Keith Dunker). 24 Purification of Intrinsically Disordered Proteins (Aviv Paz, Tzviya Zeev-Ben-Mordehai, Joel L. Sussman, and Israel Silman). INDEX.
£142.16
John Wiley & Sons Inc Chemistry and the Sense of Smell
Book SynopsisThe book provides an account of the totality of fragrance chemistry in one volume. It describes the chemistry of odorous materials, how and why they are produced in nature, how they are produced and used commercially, how they are analyzed and characterized, the chemistry of how we perceive them, and their role in our everyday lives.Trade Review“I cannot recommend this fascinating book highly enough.” (Simon Cotton, Chemistry & Industry, September 2014) “In conclusion: A comprehensive introduction to the world of odours, not only for chemists.” (review in German: Monika Paduch, Gefahrstoffe - Reinhaltung Luft, October 2014)Table of ContentsPreface vii Acknowledgments ix Introduction 1 1 Why Do We Have a Sense of Smell? 4 2 The Mechanism of Olfaction 32 3 Analysis and Characterisation of Odour 188 4 The Sense of Smell in Our Lives 209 5 The Scents of Nature 237 6 Manufacture of Fragrance Ingredients 296 7 The Design of New Fragrance Ingredients 357 8 The Relationship Between Molecular Structure and Odour 388 9 Intellectual Challenges in Fragrance Chemistry and the Future 420 Glossary 428 Index 437
£116.06
John Wiley & Sons Inc CyclicNucleotide Phosphodiesterases in the
Book SynopsisThis book reviews advances in understanding phosphodiesterases within the central nervous system and theirtherapeutic applications. A range of expert authors from both academia and industry describe these, then focus on the areas of greatest scientific and medical interest to provide more detailed coverage. Therapeutic and drug discovery applications are covered for diseasesincluding Alzheimer''s, Parkinson''s, schizophrenia, erectile dysfunction, and spinal cord injuries. There is also a chapter on drug discovery tools such as in vitro assays and X-ray structures for medicinal chemistry studies.Table of ContentsPREFACE vii CONTRIBUTORS ix 1 PHOSPHODIESTERASES AND CYCLIC NUCLEOTIDE SIGNALING IN THE CNS 1 Marco Conti and Wito Richter 2 PUTTING TOGETHER THE PIECES OF PHOSPHODIESTERASE DISTRIBUTION PATTERNS IN THE BRAIN: A JIGSAW PUZZLE OF CYCLIC NUCLEOTIDE REGULATION 47 Michy P. Kelly 3 COMPARTMENTALIZATION AND REGULATION OF CYCLIC NUCLEOTIDE SIGNALING IN THE CNS 59 Manuela Zaccolo and Alessandra Stangherlin 4 PHARMACOLOGICAL MANIPULATION OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASE SIGNALING FOR THE TREATMENT OF NEUROLOGICAL AND PSYCHIATRIC DISORDERS IN THE BRAIN 77 Frank S. Menniti, Niels Plath, Niels Svenstrup, and Christopher J. Schmidt 5 RECENT RESULTS IN PHOSPHODIESTERASE INHIBITOR DEVELOPMENT AND CNS APPLICATIONS 115 David P. Rotella 6 CRYSTAL STRUCTURES OF PHOSPHODIESTERASES AND IMPLICATION ON DISCOVERY OF INHIBITORS 145 Hengming Ke, Huanchen Wang, Mengchun Ye, and Yingchun Huang 7 INHIBITION OF CYCLIC NUCLEOTIDE PHOSPHODIESTERASES TO REGULATE MEMORY 171 Han-Ting Zhang, Ying Xu, and James O’Donnell 8 EMERGING ROLE FOR PDE4 IN NEUROPSYCHIATRIC DISORDERS: TRANSLATING ADVANCES FROM GENETIC STUDIES INTO RELEVANT THERAPEUTIC STRATEGIES 211 Sandra P. Zoubovsky, Nicholas J. Brandon, and Akira Sawa 9 BEYOND ERECTILE DYSFUNCTION: UNDERSTANDING PDE5 ACTIVITY IN THE CENTRAL NERVOUS SYSTEM 223 Eva P.P. Bollen, Kris Rutten, Olga A.H. Reneerkens, Harry M.W. Steinbusch, and Jos Prickaerts 10 MOLECULAR AND CELLULAR UNDERSTANDING OF PDE10A: A DUAL-SUBSTRATE PHOSPHODIESTERASE WITH THERAPEUTIC POTENTIAL TO MODULATE BASAL GANGLIA FUNCTION 247 Erik I. Charych and Nicholas J. Brandon 11 ROLE OF CYCLIC NUCLEOTIDE SIGNALING AND PHOSPHODIESTERASE ACTIVATION IN THE MODULATION OF ELECTROPHYSIOLOGICAL ACTIVITY OF CENTRAL NEURONS 269 Sarah Threlfell and Anthony R. West 12 THE ROLE OF PHOSPHODIESTERASES IN DOPAMINE SYSTEMS GOVERNING MOTIVATED BEHAVIOR 303 Gretchen L. Snyder, Joseph P. Hendrick, and Akinori Nishi 13 INHIBITION OF PHOSPHODIESTERASES AS A STRATEGY FOR TREATMENT OF SPINAL CORD INJURY 353 Elena Nikulina and Marie T. Filbin INDEX 375
£100.76
John Wiley & Sons Inc Modern Tools for the Synthesis of Complex
Book SynopsisAn overview of the new technologies that have revolutionized organic chemistry and allowed easy access to complex bioactives, this book brings modern synthetic techniques and bioactives together. The synthesis of structurally complex molecules has become a real challenge for the synthetic community.Table of ContentsFOREWORD vii PREFACE ix CONTRIBUTORS xi CHAPTER 1 C–H FUNCTIONALIZATION: A NEW STRATEGY FOR THE SYNTHESIS OF BIOLOGICALLY ACTIVE NATURAL PRODUCTS 1 Sophie Rousseaux, Benoýˆt Lie´gault, and Keith Fagnou CHAPTER 2 THE NEGISHI CROSS-COUPLING IN THE SYNTHESIS OF NATURAL PRODUCTS AND BIOACTIVE MOLECULES 33 Evelina Colacino, Jean Martinez, and Fre´de´ric Lamaty CHAPTER 3 METAL-CATALYZED C–HETEROATOM CROSS-COUPLING REACTIONS 77 Renata Marcia de Figueiredo, Jean Marc Campagne, and Damien Prim CHAPTER 4 GOLDEN OPPORTUNITIES IN THE SYNTHESIS OF NATURAL PRODUCTS AND BIOLOGICALLY ACTIVE COMPOUNDS 111 Fabien Gagosz CHAPTER 5 METATHESIS-BASED SYNTHESIS OF COMPLEX BIOACTIVES 155 Jean-Alexandre Richard, Sin Yee Ng, and David Y.-K. Chen CHAPTER 6 ENANTIOSELECTIVE ORGANOCATALYSIS: A POWERFUL TOOL FOR THE SYNTHESIS OF BIOACTIVE MOLECULES 189 Mitsuru Shoji and Yujiro Hayashi CHAPTER 7 ASYMMETRIC PHASE-TRANSFER CATALYSIS 213 Seiji Shirakawa, Shin A. Moteki, and Keiji Maruoka CHAPTER 8 REARRANGEMENTS IN NATURAL PRODUCT SYNTHESIS 243 Jose´ Marco-Contelles and Elena Soriano CHAPTER 9 DOMINO REACTIONS IN THE ENANTIOSELECTIVE SYNTHESIS OF BIOACTIVE NATURAL PRODUCTS 271 Lutz F. Tietze, Scott G. Stewart, and Alexander Du¨fert CHAPTER 10 FLUOROUS LINKER-FACILITATED SYNTHESIS OF BIOLOGICALLY INTERESTING MOLECULES 335 Wei Zhang CHAPTER 11 THE EVOLUTION OF IMMOBILIZED REAGENTS AND THEIR APPLICATION IN FLOW CHEMISTRY FOR THE SYNTHESIS OF NATURAL PRODUCTS AND PHARMACEUTICAL COMPOUNDS 359 Rebecca M. Myers, Kimberley A. Roper, Ian R. Baxendale, and Steven V. Ley CHAPTER 12 SYNTHETIC APPROACHES TO BIOACTIVE CARBOHYDRATES 395 Xavier Guinchard, Se´bastien Picard, and David Crich CHAPTER 13 AMMONIUM YLIDES AS BUILDING BLOCKS FOR ALKALOID SYNTHESIS 433 Scott Bur and Albert Padwa CHAPTER 14 PRECURSOR-DIRECTED BIOSYNTHESIS OF POLYKETIDE AND NONRIBOSOMAL PEPTIDE NATURAL PRODUCTS 485 Colin J. B. Harvey and Chaitan Khosla CHAPTER 15 TARGET-ORIENTED AND DIVERSITY-ORIENTED ORGANIC SYNTHESIS 513 Raphae¨l Rodriguez CHAPTER 16 DNA AS A TOOL FOR MOLECULAR DISCOVERY 539 Michael Smietana, Jean-Jacques Vasseur, Janine Cossy, and Stellios Arseniyadis INDEX 557
£128.66
John Wiley & Sons Inc The Chemistry and Biology of Volatiles
Book SynopsisComing to a conclusion, this wonderful, informative and very interesting book presents an excellent overview of small volatile organic compounds and their role in our life and environment. Really fascinating is the entirety of scientific disciplines which were addressed by this book.Trade Review"Coming to a conclusion, this wonderful, informative and very interesting book presents an excellent overview of small volatile organic compounds and their role in our life and environment. Really fascinating is the entirety of scientific disciplines which were addressed by this book." (Flavour and Fragrance Journal, 2011) "In spite of its few shortcomings, this book deserves to be a well-used reference in the library of any laboratory specialising in VOC". (Chemistry World, 1 May 2011) "The Chemistry and Biology of Volatiles takes an interdisciplinary approach to volatile molecules". (Small Business VoIP, 14 December 2010)Table of ContentsForeword xiii List of Contributors xv Acknowledgements xvii Abbreviations xix 1 Volatiles – An Interdisciplinary Approach 1 Andreas Herrmann 1.1 Introduction 1 1.2 Geraniol – A Typical Example 2 1.3 Conclusion 8 References 8 2 Biosynthesis and Emission of Isoprene, Methylbutanol and Other Volatile Plant Isoprenoids 11 Hartmut K. Lichtenthaler 2.1 Introduction 11 2.2 Plant Isoprenoids 12 2.3 Two IPP-Yielding Pathways in Plants 15 2.4 Prenyl Chain Formation and Elongation 16 2.5 Compartmentation of Plant Isoprenoid Biosynthesis 16 2.6 The Enzyme Steps of the Plastidic DOXP/MEP Pathway of IPP Formation 17 2.7 Cross-Talk Between the Two IPP Biosynthesis Pathways 19 2.8 Biosynthesis and Emission of Volatile Isoprene at High Irradiance 22 2.8.1 Regulation of Isoprene Emission 25 2.9 Inhibition of Isoprene Biosynthesis 26 2.9.1 Fosmidomycin and 5-Ketoclomazone 26 2.9.2 Diuron 27 2.10 Inhibition of Carotenoid and Chlorophyll Biosynthesis by Fosmidomycin and 5-Ketoclomazone 27 2.11 Biosynthesis and Emission of Methylbutenol at High Irradiance 28 2.12 Source of Pyruvate for Isoprene and Methylbutenol Biosynthesis 29 2.13 Branching Point of DOXP/MEP Pathway with Other Metabolic Chloroplast Pathways 30 2.14 Is There a Physiological Function of Isoprene and MBO Emission? 31 2.15 Biosynthesis and Emission of Monoterpenes, Sesquiterpenes and Diterpenes 33 2.15.1 Monoterpenes 35 2.15.2 Diterpenes 36 2.15.3 Sesquiterpenes 36 2.16 Some General Remarks on the Regulation of Terpene Biosynthesis in Plants 36 2.17 Volatile Terpenoids as Aroma Compounds of Wine 37 2.18 Function of Terpenes in Plant Defence 38 2.19 Conclusion 38 Acknowledgements 39 References 40 3 Analysis of the Plant Volatile Fraction 49 Patrizia Rubiolo, Barbara Sgorbini, Erica Liberto, Chiara Cordero and Carlo Bicchi 3.1 Introduction 49 3.2 Sample Preparation 50 3.2.1 ‘Liquid’ Phase Sampling 51 3.2.2 Headspace Sampling 51 3.2.3 Headspace–Solid Phase Microextraction 52 3.2.4 In-Tube Sorptive Extraction 54 3.2.5 Headspace Sorptive Extraction 55 3.2.6 Static and Trapped Headspace 56 3.2.7 Solid-Phase Aroma Concentrate Extraction 56 3.2.8 Headspace Liquid-Phase Microextraction 56 3.2.9 Large Surface Area High Concentration Capacity Headspace Sampling 59 3.3 Analysis 59 3.3.1 Fast-GC and Fast-GC-qMS EO Analysis 61 3.3.2 Qualitative Analysis 65 3.3.3 Quantitative Analysis 66 3.3.4 Enantioselective GC 70 3.3.5 Multidimensional GC Techniques 75 3.4 Further Developments 76 3.5 Conclusion 85 Acknowledgements 87 References 87 4 Plant Volatile Signalling: Multitrophic Interactions in the Headspace 95 Andre Kessler and Kimberly Morrell 4.1 Introduction 95 4.2 The Specificity and Complexity of Herbivore-Induced VOC Production 97 4.2.1 Plant Endogenous Wound Signalling 99 4.2.2 Herbivore-Derived Elicitors of VOC Emission 102 4.3 Ecological Consequences of VOC Emission 104 4.3.1 Within-Plant Defence Signalling 104 4.3.2 Herbivore-Induced VOC Emission as Part of a Metabolic Reconfiguration of the Plant 105 4.3.3 Herbivores Use VOCs to Select Host Plants 107 4.3.4 VOCs as Indirect Defences Against Herbivores 108 4.3.5 VOCs in Plant–Plant Interactions 111 4.4 Conclusion 112 Acknowledgements 114 References 114 5 Pheromones in Chemical Communication 123 Kenji Mori 5.1 Introduction 123 5.1.1 Definition of Pheromones 123 5.1.2 Classification of Pheromones 123 5.2 History of Pheromone Research 125 5.3 Research Techniques in Pheromone Science 127 5.3.1 The Collecting of Pheromones 127 5.3.2 Bioassay-Guided Purification 128 5.3.3 Structure Determination and Synthesis 128 5.3.4 Field Bioassay 129 5.3.5 Structure Elucidation of the Male-Produced Aggregation Pheromone of the Stink Bug Eysarcoris lewisi – A Case Study 129 5.4 Structural Diversity Among Pheromones 132 5.5 Complexity of Multicomponent Pheromones 137 5.6 Stereochemistry and Pheromone Activity 139 5.6.1 Only a Single Enantiomer is Bioactive and its Opposite Enantiomer Does Not Inhibit the Response to the Active Isomer 139 5.6.2 Only One Enantiomer is Bioactive, and its Opposite Enantiomer Inhibits the Response to the Pheromone 139 5.6.3 Only One Enantiomer is Bioactive, and its Diastereomer Inhibits the Response to the Pheromone 139 5.6.4 The Natural Pheromone is a Single Enantiomer, and its Opposite Enantiomer or Diastereomer is Also Active 140 5.6.5 The Natural Pheromone is a Mixture of Enantiomers or Diastereomers, and Both of the Enantiomers, or All of the Diastereomers are Separately Active 141 5.6.6 Different Enantiomers or Diastereomers are Employed by Different Species 141 5.6.7 Both Enantiomers are Necessary for Bioactivity 141 5.6.8 One Enantiomer is More Active Than the Other, but an Enantiomeric or Diastereomeric Mixture is More Active Than the Enantiomer Alone 141 5.6.9 One Enantiomer is Active on Males, While the Other is Active on Females 142 5.6.10 Only the meso-Isomer is Active 142 5.7 Pheromones With Kairomonal Activities 142 5.8 Mammalian Pheromones 143 5.9 Invention of Pheromone Mimics 145 5.10 Conclusion 147 Acknowledgements 147 References 147 6 Use of Volatiles in Pest Control 151 J. Richard M. Thacker and Margaret R. Train 6.1 Introduction 151 6.2 Repellents (DEET, Neem, Essential Oils) 151 6.3 Volatile Synthetic Chemicals and Fumigants 154 6.4 Pheromones 158 6.5 Volatile Allelochemicals 165 6.6 Plant Volatiles and Behavioural Modification of Beneficial Insects 166 6.7 Concluding Comments 167 References 168 7 Challenges in the Synthesis of Natural and Non-Natural Volatiles 173 Anthony A. Birkbeck 7.1 Introduction – The Art of Organic Synthesis 173 7.2 Overcoming Challenges in the Small-Scale Synthesis of Natural Volatile Compounds 174 7.2.1 D,L-Caryophyllene (1964) 174 7.2.2 b-Vetivone (1973) 175 7.3 Overcoming Challenges in the Large-Scale Synthesis of Nature Identical and Non-Natural Molecules 176 7.3.1 (Z)-3-Hexenol 176 7.3.2 Citral 177 7.3.3 (–)-Menthol 179 7.3.4 Habanolide 180 7.4 Remaining Challenges in the Large-Scale Synthesis of Natural and Non-Natural Volatiles 180 7.5 Design and Synthesis of Novel Odorants and Potential Industrial Routes to a Natural Product 182 7.5.1 Cassis (Blackcurrant) 182 7.5.2 Patchouli 184 7.5.3 Musk 187 7.5.4 Sandalwood 189 7.6 Other Challenges 193 7.7 Conclusion 193 Acknowledgements 194 Dedication 195 References 195 8 The Biosynthesis of Volatile Sulfur Flavour Compounds 203 Meriel G. Jones 8.1 Introduction: Flavours as Secondary Metabolites 203 8.2 Sulfur in Plant Biology 204 8.3 Sulfur Compounds as Flavour Volatiles 205 8.4 The Alk(en)yl Cysteine Sulfoxide Flavour Precursors 206 8.5 Biosynthesis of the Flavour Precursors of Allium 207 8.5.1 The Biosynthesis of Allium Flavour Precursors via g-Glutamyl Peptides 208 8.5.2 The Biosynthesis of Allium Flavour Precursors via Cysteine Synthases 209 8.6 Formation of Volatiles from CSOs 210 8.6.1 S-Methyl-L-cysteine sulfoxide 210 8.6.2 Release of the Allium CSOs 211 8.7 The Allium Flavour Volatiles 212 8.8 The Enzyme Alliinase 213 8.9 The Enzyme Lachrymatory Factor Synthase 214 8.10 The Biological Roles of the Flavour Precursors 215 8.11 The Glucosinolate Flavour Precursors 216 8.12 GS and Their Biosynthetic Pathways 216 8.13 Release of Volatile GS Hydrolysis Products 218 8.14 The Biological Role of Glucosinolates 220 8.15 Application of Transgenic Technology to Applied Aspects of GS Biosynthesis 222 8.16 Volatile Sulfur Compounds from Other Plants 222 8.16.1 Complex Organic Sulfur Volatiles 222 8.16.2 Simple Sulfur Volatiles 223 8.16.3 Hydrogen Sulfide 223 8.16.4 Methanethiol 224 8.17 Conclusion 224 References 224 9 Thermal Generation of Aroma-Active Volatiles in Food 231 Christoph Cerny 9.1 Introduction 231 9.2 The Maillard Reaction 233 9.2.1 The Amadori Rearrangement 234 9.2.2 Deoxyosones 235 9.2.3 Retro-Aldolization 235 9.3 Formation of Aroma Compounds in the Later Stages of the Maillard Reaction 237 9.3.1 2-Furfurylthiol 237 9.3.2 4-Hydroxy-2,5-dimethyl-3(2H)-furanone 239 9.3.3 Alkyl and Alkenylpyrazines 239 9.3.4 2-Acetyl-1-pyrroline 241 9.4 The Strecker Degradation 241 9.5 Caramelization 244 9.6 Thiamin Degradation 246 9.7 Ferulic Acid Degradation 246 9.8 Fat Oxidation 247 9.9 Conclusion 250 References 250 10 Human Olfactory Perception 253 Alan Gelperin 10.1 Introduction 253 10.2 Historical Perspective on Olfactory Perception 254 10.3 Human Olfactory Pathway 255 10.4 Functional Studies in Human Subjects 256 10.5 Functional Studies in Brain-Damaged Subjects 259 10.6 Single Odorants, Binary Mixtures and Complex Odour Objects 259 10.7 Olfactory Versus Trigeminal Odorant Identification 262 10.8 Orthonasal Versus Retronasal Odour Perception 263 10.9 Specific Anosmias 264 10.10 MHC-Correlated Odour Preferences in Human Subjects 265 10.11 Odour Deprivation and Odour Perception 266 10.12 Age-Related Decline in Olfactory Perception 267 10.13 New Neurons in Adult Brains 268 10.14 Epidemiological Studies of Human Olfaction 268 10.15 Active Sampling and Olfactory Perception 269 10.16 Human Olfactory Imagery 270 10.17 Top-Down Influences on Olfactory Perception 271 10.18 Reproductive State and Olfactory Sensitivity 272 10.19 Olfaction, Hunger and Satiety 273 10.20 Odour Perception Bias by Odour Names 274 10.21 Olfaction and Disease States 275 10.22 Prenatal and Postnatal Influences on Infant Odour/Flavour Preferences 276 10.23 Future Directions 277 Acknowledgements 277 References 278 11 Perfumery – The Wizardry of Volatile Molecules 291 Christophe Laudamiel 11.1 The Big Picture 291 11.2 Wizardry No. 1: Full Holograms Create Real Emotions 292 11.3 Volatiles Need a Language Wizard 296 11.4 Wizardry No. 2: The Perfumer in the Jungle of Volatiles to Create Emotions 298 11.5 Wizardry No. 3: End Results Are Music to the Nose 303 References 304 12 Microencapsulation Techniques for Food Flavour 307 Youngjae Byun, Young Teck Kim, Kashappa Goud H. Desai and Hyun Jin Park 12.1 Demands 307 12.2 Microencapsulation in the Food Industry 307 12.3 Techniques and Materials for Flavour Microencapsulation 308 12.3.1 Spray Drying 308 12.3.2 Extrusion 312 12.3.3 Cyclodextrin Inclusion Complexes 314 12.3.4 Helical Inclusion Complexes 316 12.3.5 Fluidized Bed Coating 318 12.3.6 Top Spray Fluidized Bed Coating 318 12.3.7 Bottom Spray System 318 12.3.8 Wurster System 320 12.3.9 Tangential Spray or Rotary Fluidized Bed Coating 320 12.3.10 Coacervation 320 12.3.11 Double or Multiple Emulsion with Freeze Drying 321 12.3.12 Co-Crystallization 322 12.3.13 Spray Chilling and Spray Cooling 322 12.3.14 Supercritical Fluids 323 12.3.15 Other Techniques 323 12.4 Conclusion and Future Trends 325 References 326 13 Profragrances and Properfumes 333 Andreas Herrmann 13.1 Introduction 333 13.2 Release of Alcohols 335 13.2.1 Enzymatic Hydrolysis 335 13.2.2 Neighbouring-Group-Assisted, Non-Enzymatic Hydrolysis 340 13.3 Release of Carbonyl Derivatives 346 13.3.1 Oxidations 346 13.3.2 Reversible Systems 350 13.3.3 Retro 1,4-Additions 354 13.4 Profragrance and Properfume Strategies 356 13.4.1 Performance and Cost Efficiency 356 13.4.2 Stability 357 13.5 Conclusion 357 Acknowledgements 358 References 358 14 Reactions of Biogenic Volatile Organic Compounds in the Atmosphere 363 Russell K. Monson 14.1 Introduction 363 14.2 The Relative Importance of Anthropogenic Versus Biogenic VOC Emissions to Atmospheric Chemistry 364 14.3 Overview of BVOC Oxidation 365 14.4 The Types of Emitted BVOCs and General Roles in Atmospheric Chemistry 370 14.5 Gas Phase Oxidation of BVOCs 372 14.6 Gas Phase Chemistry of BVOCs in Urban and Suburban Airsheds 374 14.7 Gas Phase Chemistry Within and Above Forests 375 14.8 BVOC Emissions and SOA Formation 377 14.9 Conclusion 381 References 381 Index 389
£107.06
John Wiley and Sons Ltd Biopharmaceuticals
Book SynopsisThe latest edition of this highly acclaimed textbook, provides a comprehensive and up--to--date overview of the science and medical applications of biopharmaceutical products.Trade Review"…contains just about everything that anyone would want to know about the subject…It's all here in this easy-to-read textbook.” (Biochemistry and Molecular Education, March/April 2004) "...well written… (and) copiously illustrated..." (Chemistry & Industry, 17th January 2005) “This book should be recommended reading for all under-graduate course in pharmacy and the pharmaceutical sciences …”. (Cell Biochemistry & Function, March-April 2005)Table of ContentsPreface xvii Chapter 1 Pharmaceuticals, biologics and biopharmaceuticals 1 Introduction to pharmaceutical products 1 Biopharmaceuticals and pharmaceutical biotechnology 1 History of the pharmaceutical industry 3 The age of biopharmaceuticals 5 Biopharmaceuticals: current status and future prospects 8 Traditional pharmaceuticals of biological origin 12 Pharmaceuticals of animal origin 13 The sex hormones 14 The androgens 14 Oestrogens 15 Progesterone and progestogens 17 Corticosteroids 19 Catecholamines 21 Prostaglandins 23 Pharmaceutical substances of plant origin 27 Alkaloids 28 Atropine and scopalamine 28 Morphine and cocaine 29 Additional plant alkaloids 30 Ergot alkaloids 30 Flavonoids, xanthines and terpenoids 30 Cardiac glycosides and coumarins 33 Aspirin 33 Pharmaceutical substances of microbial origin 33 The macrolides and ansamycins 38 Peptide and other antibiotics 39 Conclusion 39 Further reading 40 Chapter 2 The drug development process 43 Drug discovery 44 The impact of genomics and related technologies upon drug discovery 45 Gene chips 47 Proteomics 49 Structural genomics 50 Pharmacogenetics 51 Plants as a source of drugs 52 Microbial drugs 53 Rational drug design 54 Combinatorial approaches to drug discovery 56 Initial product characterization 57 Patenting 57 What is a patent and what is patentable? 57 Patent types 62 The patent application 63 Patenting in biotechnology 64 Delivery of biopharmaceuticals 66 Oral delivery systems 66 Pulmonary delivery 67 Nasal, transmucosal and transdermal delivery systems 68 Pre-clinical trials 69 Pharmacokinetics and pharmacodynamics 69 Toxicity studies 71 Reproductive toxicity and teratogenicity 71 Mutagenicity, carcinogenicity and other tests 72 Clinical trials 73 Clinical trial design 75 Trial size and study population 75 Randomized control studies 76 Additional trial designs 76 The role and remit of regulatory authorities 78 The Food and Drug Administration 78 The investigational new drug application 80 The new drug application 82 European regulations 84 National regulatory authorities 84 The EMEA and the new EU drug approval systems 85 The centralized procedure 86 Mutual recognition 88 Drug registration in Japan 88 World harmonization of drug approvals 89 Conclusion 89 Further reading 89 Chapter 3 The drug manufacturing process 93 International pharmacopoeia 93 Martindale, the Extra Pharmacopoeia 94 Guides to good manufacturing practice 94 The manufacturing facility 97 Clean rooms 98 Cleaning, decontamination and sanitation (CDS) 101 CDS of the general manufacturing area 102 CDS of process equipment 102 Water for biopharmaceutical processing 104 Generation of purified water and water for injections (WFI) 105 Distribution system for WFI 107 Documentation 109 Specifications 110 Manufacturing formulae, processing and packaging instructions 110 Records 111 Generation of manufacturing records 111 Sources of biopharmaceuticals 112 E. coli as a source of recombinant, therapeutic proteins 112 Expression of recombinant proteins in animal cell culture systems 116 Additional production systems: yeasts 116 Fungal production systems 117 Transgenic animals 118 Transgenic plants 122 Insect cell-based systems 123 Production of final product 124 Cell banking systems 127 Upstream processing 128 Microbial cell fermentation 129 Mammalian cell culture systems 133 Downstream processing 134 Final product formulation 140 Some influences that can alter the biological activity of proteins 142 Proteolytic degradation 143 Protein deamidation 144 Oxidation and disulphide exchange 145 Alteration of glycoprotein glycosylation patterns 147 Stabilizing excipients used in final product formulations 150 Final product fill 153 Freeze-drying 155 Labelling and packing 158 Analysis of the final product 159 Protein-based contaminants 159 Removal of altered forms of the protein of interest from the product stream 160 Product potency 161 Determination of protein concentration 163 Detection of protein-based product impurities 164 Capillary electrophoresis 166 High-pressure liquid chromatography (HPLC) 167 Mass spectrometry 168 Immunological approaches to detection of contaminants 168 Amino acid analysis 169 Peptide mapping 170 N-terminal sequencing 171 Analysis of secondary and tertiary structure 173 Endotoxin and other pyrogenic contaminants 173 Endotoxin, the molecule 174 Pyrogen detection 176 Dna 179 Microbial and viral contaminants 180 Viral assays 181 Miscellaneous contaminants 182 Validation studies 183 Further reading 185 Chapter 4 The cytokines —the interferon family 189 Cytokines 189 Cytokine receptors 194 Cytokines as biopharmaceuticals 195 The interferons 196 The biochemistry of interferon-a 197 Interferon-b 198 Interferon-g 198 Interferon signal transduction 198 The interferon receptors 199 The JAK–STAT pathway 199 The interferon JAK–STAT pathway 202 The biological effects of interferons 203 The eIF-2a protein kinase system 207 Interferon biotechnology 207 Production and medical uses of IFN-a 210 Medical uses of IFN-b 213 Medical applications of IFN-g 214 Interferon toxicity 216 Additional interferons 218 Conclusion 219 Further reading 219 Chapter 5 Cytokines: interleukins and tumour necrosis factor 223 Interleukin-2 (IL-2) 225 IL-2 production 228 IL-2 and cancer treatment 228 IL-2 and infectious diseases 230 Safety issues 231 Inhibition of IL-2 activity 231 Interleukin-1 (IL-1) 232 The biological activities of IL- 1 233 IL-1 biotechnology 234 Interleukin-3: biochemistry and biotechnology 235 Interleukin- 4 236 Interleukin- 6 238 Interleukin- 11 240 Interleukin- 5 241 Interleukin- 12 244 Tumour necrosis factors (TNFs) 246 TNF biochemistry 246 Biological activities of TNF-a 247 Immunity and inflammation 248 TNF receptors 249 TNF: therapeutic aspects 250 Further reading 252 Chapter 6 Haemopoietic growth factors 255 The interleukins as haemopoietic growth factors 257 Granulocyte colony stimulating factor (G-CSF) 258 Macrophage colony-stimulating factor (M-CSF) 259 Granulocyte-macrophage colony stimulating factor (GM-CSF) 259 Clinical application of CSFs 261 Leukaemia inhibitory factor (LIF) 263 Erythropoietin (EPO) 264 The EPO receptor and signal transduction 267 Regulation of EPO production 267 Therapeutic applications of EPO 268 Chronic disease and cancer chemotherapy 271 Additional non-renal applications 272 Tolerability 273 Thrombopoietin 273 Further reading 275 Chapter 7 Growth factors 277 Growth factors and wound healing 277 Insulin-like growth factors (IGFs) 279 IGF biochemistry 280 IGF receptors 280 IGF-binding proteins 282 Biological effects 282 IGF and fetal development 283 IGFs and growth 283 Renal and reproductive effects 284 Neuronal and other effects 285 Epidermal growth factor (EGF) 285 The EGF receptor 286 Platelet-derived growth factor (PDGF) 287 The PDGF receptor and signal transduction 288 PDGF and wound healing 289 Fibroblast growth factors (FGFs) 289 Transforming growth factors (TGFs) 290 TGF-a 290 TGF-b 292 Neurotrophic factors 293 The neurotrophins 294 Neurotrophin receptors 296 The neurotrophin low-affinity receptor 297 Ciliary neurotrophic factor and glial cell line-derived neurotrophic factor 297 Neurotrophic factors and neurodegenerative disease 298 Amyotrophic lateral sclerosis (ALS) and peripheral neuropathy 298 Neurotrophic factors and neurodegenerative diseases of the brain 298 Further reading 300 Chapter 8 Hormones of therapeutic interest 303 Insulin 303 Diabetes mellitus 304 The insulin molecule 304 The insulin receptor and signal transduction 307 Insulin production 307 Enzymatic conversion of porcine insulin 311 Production of human insulin by recombinant DNA technology 312 Formulation of insulin products 314 Engineered insulins 317 Additional means of insulin administration 320 Treating diabetics with insulin-producing cells 321 Glucagon 321 Human growth hormone (hGH) 324 Growth hormone releasing factor (GHRF) and inhibitory factor (GHRIF) 325 The GH receptor 325 Biological effects of GH 327 Therapeutic uses of GH 328 Recombinant hGH (rhGH) and pituitary dwarfism 328 Idiopathic short stature and Turner’s syndrome 330 Metabolic effects of hGH 330 GH, lactation and ovulation 331 The gonadotrophins 331 Follicle stimulating hormone (FSH), luteinizing hormone (LH) and human chorionic gonadotrophin (hCG) 331 Pregnant mare serum gonadotrophin (PMSG) 335 The inhibins and activins 337 LHRH and regulation of gonadotrophin production 338 Medical and veterinary applications of gonadotrophins 339 Sources and medical uses of FSH, LHand hCG 340 Recombinant gonadotrophins 342 Veterinary uses of gonadotrophins 344 Gonadotrophin releasing hormone (GnRH) 345 Additional recombinant hormones now approved 345 Conclusions 348 Further reading 348 Chapter 9 Blood products and therapeutic enzymes 351 Disease transmission 351 Whole blood 353 Platelets and red blood cells 353 Blood substitutes 353 Dextrans 354 Albumin 355 Gelatin 357 Oxygen-carrying blood substitutes 357 Haemostasis 358 The coagulation pathway 358 Terminal steps of coagulation pathway 361 Clotting disorders 365 Factor VIII and haemophilia 366 Production of Factor VIII 368 Factors IX, VII a and XIII 371 Anticoagulants 372 Heparin 372 Vitamin Kantimetabolites 375 Hirudin 375 Antithrombin 379 Thrombolytic agents 380 Tissue plasminogen activator (tPA) 381 First-generation tPA 383 Engineered tPA 383 Streptokinase 385 Urokinase 386 Staphylokinase 386 a1 -Antitrypsin 388 Enzymes of therapeutic value 389 Asparaginase 390 DNase 392 Glucocerebrosidase 393 a-Galactosidase and urate oxidase 395 Superoxide dismutase 397 Debriding agents 397 Digestive aids 398 Lactase 400 Further reading 400 Chapter 10 Antibodies, vaccines and adjuvants 403 Polyclonal antibody preparations 403 Anti-D immunoglobulin 406 Normal immunoglobulins 407 Hepatitis Band tetanus immunoglobulin 407 Snake and spider antivenins 408 Monoclonal antibodies 409 Production of monoclonals via hybridoma technology 411 Antibody screening: phage display technology 412 Therapeutic application of monoclonal antibodies 414 Tumour immunology 415 Antibody-based strategies for tumour detection/destruction 417 Drug-based tumour immunotherapy 424 First-generation anti-tumour antibodies: clinical disappointment 426 Tumour-associated antigens 426 Antigenicity of murine monoclonals 428 Chimaeric and humanized antibodies 429 Antibody fragments 432 Additional therapeutic applications of monoclonal antibodies 433 Cardiovascular and related disease 433 Infectious diseases 433 Autoimmune disease 434 Transplantation 434 Vaccine technology 435 Traditional vaccine preparations 436 Attenuated, dead or inactivated bacteria 438 Attenuated and inactivated viral vaccines 439 Toxoids, antigen-based and other vaccine preparations 440 The impact of genetic engineering on vaccine technology 441 Peptide vaccines 444 Vaccine vectors 445 Development of an AIDS vaccine 447 Difficulties associated with vaccine development 450 AIDS vaccines in clinical trials 450 Cancer vaccines 452 Recombinant veterinary vaccines 452 Adjuvant technology 453 Adjuvant mode of action 455 Mineral-based adjuvants 455 Oil-based emulsion adjuvants 455 Bacteria/bacterial products as adjuvants 457 Additional adjuvants 458 Further reading 460 Chapter 11 Nucleic acid therapeutics 463 Gene therapy 463 Basic approach to gene therapy 464 Some additional questions 467 Vectors used in gene therapy 468 Retroviral vectors 468 Additional viral-based vectors 472 Manufacture of viral vectors 474 Non-viral vectors 476 Manufacture of plasmid DNA 480 Gene therapy and genetic disease 482 Gene therapy and cancer 485 Gene therapy and AIDS 486 Gene-based vaccines 488 Gene therapy: some additional considerations 488 Anti-sense technology 488 Anti-sense oligonucleotides 490 Uses, advantages and disadvantages of ‘oligos’ 491 Delivery and cellular uptake of oligonucleotides 493 Manufacture of oligonucleotides 493 Vitravene, an approved antisense agent 494 Antigene sequences and ribozymes 494 Conclusion 495 Further reading 496 Appendix 1 Biopharmaceuticals thus far approved in the USA or European Union 499 Appendix 2 Some Internet addresses relevant to the biopharmaceutical sector 509 Appendix 3 Two selected monographs reproduced from the European Pharmacopoeia with permission from the European Commission: I. Products of recombinant DNA technology 515 II. Interferon a-2 concentrated solution 520 Appendix 4 Manufacture of biological medicinal products for human use. (Annex 2 from The Rules Governing Medicinal Products in the European Community, Vol. 4, Good Manufacturing Practice for Medicinal Products) 527 Index 533
£81.65
John Wiley & Sons Inc Methods in Molecular Biology Protein Chemistry
Book SynopsisThe advances made in molecular biology have allowed scientists to manipulate DNA. This means that molecular biology has become a tool for answering scientific questions that may be quite unrelated to genetics and cell biology. The techniques have become a means to an end, rather than an end in themselves. This work deals with this area.Table of ContentsPreface Introduction and Background Part 1 Molecular Biology Experiment 1 Instructions for Pipetman Use Experiment 2 Preparation of Plasmid Template DNA Experiment 3 Estimation of Plasmid DNA Concentration PCR Amplification of LTB Gene from Plasmid DNA Experiment 4 Agarose Gel Eletrophoresis Recovery of PCR Product Experiment 5 Restriction Digest of LTB Insert Plate Preparation Experiment 6 Purification of Digested Insert and Vector by Agarose Gel Eletrophoresis Recovery of Digested Insert and Vector Experiment 7 Determination of DNA Concentrations Ligation of Insert into Vector Experiment 8 Transformation of Host Cells by Construct Experiment 9 Colony-pick PCR to Check for Presence of Insert Streak Plates with Potential Constructs Inoculate Cultures/Run PCR Experiment 10 Agarose Gel Electrophoresis to Verify Presence of Insert Purification of the Plasmid Construct using a Plasmid Miniprep Kit Experiment 11 DNA Concentration Determination Ethanol Precipitation of Plasmid DNA Sequencing Reaction Experiment 12 Purification of Extension Reaction Products Sequencing Gel Demonstration Experiment 13 Analysis of Sequencing Data Verification of Insert Sequence Experiment 14 Gene Expression Transformation into Expression Host Experiment 15 Induction of LTB Gene Expression Part 2 Protein Chemistry Experiment 16 SDS-PAGE of Induction Time Course Transfer of Protein to Membrane for Western Blot Experiment 17 Visualization of Western Blot Experiment 18 Large Scale Culture Preparation Experiment 19 Isolation of LTB by Affinity Chromatography Experiment 20 SDS-PAGE of Column Fractions Immunoblot to verify Presence of LTB Experiment 21 Protein Concentration Protein Crystallization Experiment 22 Setting up an ELISA Microtiter Plate Experiment 23 ELISA of a Panel of Ganglioside Ligands Experiment 24 Protein Structure Determination: X-ray Diffraction Techniques Experiment 25 Characterization of a Protein Crystal Experiment 26 Primer Design
£111.56
John Wiley & Sons Inc Immunoinformatics
Book SynopsisThe astounding diversity of the immune system and the complexity of its regulatory pathways makes immunology a combinatorial science. Computational analysis has therefore become an essential element of immunology research and this has led to the creation of the emerging field of immunoinformatics. This book is the first to feature thorough coverage of this new field. Immunoinformatics facilitates the understanding of immune function by modelling the interactions among immunological components. Biological research provides ever deeper insights into the complexity of living organisms while computer science provides an effective means to store and analyse large volumes of complex data. Combining the two fields increases the efficiency of biological research and offers the potential for major advances in the study of biological systems. This book encompasses key developments in immunoinformatics, including immunological databases, sequence analysis, structure modelling, mathematiTable of ContentsChair's Introduction (H. Rammensee). Immunoinformatics - the new kid in town (V. Brusic & N. Petrovsky). The fututre of computational modelling and prediction systems in clinical immunology (N. Petrovsky, et al.). Immunoinformatics in personalized medicine (K. Gulukota). From immunome to vaccine: epitope mapping and vaccine design tools (A. De Groot & W. Martin). Insights from MHC-bound peptides (H. Margalit & Y. Altuvia). General discussion I. Computation vaccinology: quanitative approaches (D. Flower, et al). IMGT, the international ImMunoGeneTics information System®, http://imgt.cines.fr (M. Lefranc). Generating data for databases—the peptide repertoire of HLA molecules (S. Stevanovic, et al.). HLA nomenclature and the IMGT/HLA Sequence Database (S. Marsh). From immunogenetics to immunomics: functional prospecting of genes and transcripts (C. Schönbach). Mathematical models of HIV and the immune system (D. Wodarz). General discussion II. Immunogenomics: towards a digital immune system (S. Beck). Viral bioinformatics: computational views of host and pathogen (P. Kellam, et al.). Final general discussion Closing remarks (H. Rammensee). Index of Contributors. Subject Index.
£142.16
John Wiley and Sons Ltd Antimicrobial Resistance in the Environment
Book Synopsis* Offers textbook level introductions into basic microbiology as it relates to human and ecological risk assessment of antibiotics and antibiotic resistance genes. * The unique multidisciplinary platform is intended to integrate environmental microbiology into human and ecological risk assessment.Trade Review“This is an excellent publication, including primary data and detailed explanations of specific studies and techniques as well as review chapters. As both an introduction to a research area and a call for greater study and thought into how we regulate antimicrobial use in all spheres of society to limit further resistance, I highly recommend Antimicrobial resistance in the environment.” (Phenotype, 1 February 2013) “This book will be an ideal read for anyone seeking a comprehensive introduction to the many reservoirs and routes a resistance gene may pass through before or after its appearance in a clinical setting. Even experts in the field stand to gain knowledge regarding the complex web that is the issue of antibiotic resistance in the environment.” (The Quarterly Review of Biology, 1 December 2012) Table of ContentsPREFACE xi CONTRIBUTORS xv PART I SOURCES 1 Chapter 1 Introduction 3 Stuart B. Levy Chapter 2 Path to Resistance 7 Vivian Miao, Dorothy Davies, and Julian Davies Chapter 3 Antibiotic Resistome: A Framework Linking the Clinic and the Environment 15 Gerard D. Wright Chapter 4 Ecological and Clinical Consequences of Antibiotic Subsistence by Environmental Microbes 29 Gautam Dantas and Morten O. A. Sommer Chapter 5 Importance of Adaptive and Stepwise Changes in the Rise and Spread of Antimicrobial Resistance 43 Lucia Fernandez, Elena B. M. Breidenstein, and Robert E. W. Hancock Chapter 6 Environmental Reservoirs of Resistance Genes in Antibiotic-Producing Bacteria and Their Possible Impact on the Evolution of Antibiotic Resistance 73 Paris Laskaris, William H. Gaze and Elizabeth M. H. Wellington Chapter 7 Mechanisms of Bacterial Antibiotic Resistance and Lessons Learned from Environmental Tetracycline-Resistant Bacteria 93 Marilyn C. Roberts Chapter 8 Environmental Antibiotic Resistome: New Insights from Culture-Independent Approaches 123 Isabel S. Henriques, Artur Alves, Maria Jose´ Saavedra, Mark H. M. M. Montforts, and Anto´nio Correia PART II FATE 149 Chapter 9 Environmental Pollution by Antibiotic Resistance Genes 151 Jose Luis Martinez and Jorge Olivares Chapter 10 Quantifying Anthropogenic Impacts on Environmental Reservoirs of Antibiotic Resistance 173 Amy Pruden and Mazdak Arabi Chapter 11 Antibiotic Resistance in Swine-Manure-Impacted Environments 203 Joanne Chee-Sanford, Scott Maxwell, Kristy Tsau, Kelly Merrick, and Rustam Aminov Chapter 12 Antimicrobial-Resistant Indicator Bacteria in Manure and the Tracking of Indicator Resistance Genes 225 Christina S. Ho¨lzel and Karin Schwaiger Chapter 13 Municipal Wastewater as a Reservoir of Antibiotic Resistance 241 Timothy Lapara and Tucker Burch Chapter 14 Strategies to Assess and Minimize the Biological Risk of Antibiotic Resistance in the Environment 251 Thomas Schwartz Chapter 15 Antibiotic Resistance in Animals—The Australian Perspective 265 Olasumbo Ndi and Mary Barton PART III ANTIMICROBIAL SUBSTANCES AND RESISTANCE 291 Chapter 16 Detection and Occurrence of Antibiotics and Their Metabolites in Pig Manure in Bavaria (Germany) 293 Katrin Harms and Johann Bauer Chapter 17 Fate and Transport of Antibiotics in Soil Systems 309 Alistair B. A. Boxall Chapter 18 Antibiotics in the Aquatic Environment 325 Klaus Ku¨mmerer Chapter 19 Residues of Veterinary Drugs in Wild Fish 337 Thomas Heberer Chapter 20 Role of Quaternary Ammonium Compounds on Antimicrobial Resistance in the Environment 349 Ulas Tezel and Spyros G. Pavlostathis PART IV EFFECTS AND RISKS 389 Chapter 21 Human Health Importance of use of Antimicrobials in Animals and Its Selection of Antimicrobial Resistance 391 Scott A. McEwen Chapter 22 Antimicrobial Resistance Associated with Salmonid Farming 423 Claudio D. Miranda Chapter 23 Effect of Veterinary Medicines Introduced via Manure into Soil on the Abundance and Diversity of Antibiotic Resistance Genes on Their Transferability 453 Holger Heuer, Christoph Kopmann, Ute Zimmerling, Ellen Kro¨gerrecklenfort, Kristina Kleineidamm, Michael Schloter, Eva M. Top and Kornelia Smalla Chapter 24 Tracking Antibiotics and Antibiotic Resistance Genes through the Composting Process and Field Distribution of Poultry Waste: Lessons Learned 465 Patricia L. Keen and Nancy De With Chapter 25 Environmental Microbial Communities Living Under Very High Antibiotic Selection Pressure 483 Anders Janzon, Erik Kristiansson, and D. G. Joakim Larsson Chapter 26 Antibiotic Use During an Influenza Pandemic: Downstream Ecological Effects and Antibiotic Resistance 503 Andrew C. Singer and Heike Schmitt Chapter 27 Use of Veterinary Antibacterial Agents in Europe and the United States 539 Ingeborg M. van Geijlswijk, Nico Bondt, Linda F. Puister-Jansen, and Dik J. Mevius Chapter 28 Regulatory Research on Antimicrobial Resistance in the Environment 549 Emily A. McVey and Mark H. M. M. Montforts INDEX 569
£121.46
John Wiley & Sons Inc Plant Chemical Biology
Book SynopsisThe application of plant chemical biology is currently limited to specialized subfields of plant research.Table of ContentsPreface vii Contributors ix part one Introduction 1 1.1 From Herbal Remedies to Cutting-Edge Science: A Historical Perspective of Plant Chemical Biology 3 Michelle Q. Brown, Abel Rosado, and Natasha V. Raikhel part Two Sources of small molecules 19 2.1 Compound Collections 21 Reg Richardson 2.2 Combinatorial Chemistry Library Design 40 Robert Klein and Stephen D. Lindell 2.3 Natural Product-Based Libraries 64 Alan L. Harvey part three Identification of new chemical tools by High-Throughput Screening 73 3.1 Assay Design for High-Throughput Screening 75 Frank W. An and Jose R. Perez part four Use of chemical biology to study plant physiology 93 4.1 Use of Chemical Biology to Understand Auxin Metabolism, Signaling, and Polar Transport 95 Ken-ichiro Hayashi and Paul Overvoorde 4.2 Brassinosteroids Signaling and Biosynthesis 128 Takeshi Nakano and Tadao Asami 4.3 Chemical Genetic Approaches on ABA Signal Transduction 145 Eunjoo Park and Tae-Houn Kim 4.4 Jasmonic Acid 160 Christian Meesters and Erich Kombrink 4.5 Chemical Genetics as a Tool to Study Ethylene Biology in Plants 184 Yuming Hu, Filip Vandenbussche, and Dominique Van Der Straeten part five Use of chemical biology to study plant cellular processes 203 5.1 The Use of Small Molecules to Dissect Cell Wall Biosynthesis and Manipulate the Cortical Cytoskeleton 205 Darby Harris and Seth DeBolt 5.2 The Use of Chemical Biology to Study Plant Cellular Processes: Subcellular Trafficking 218 Ash Haeger, Malgorzata £angowska, and Stéphanie Robert part six Target identification 233 6.1 Target Identification of Biologically Active Small Molecules 235 Paul Overvoorde and Dominique Audenaert part seven Translation of plant chemical biology from the lab to the field 247 7.1 Prospects and Challenges for Translating Emerging Insights in Plant Chemical Biology into New Agrochemicals 249 Terence A. Walsh 7.2 In Vitro Propagation 263 Hans Motte, Stefaan Werbrouck, and Danny Geelen Index 289
£104.36
John Wiley & Sons Inc Infrared Spectroscopy of Biomolecules
Book SynopsisThis edited volume spans the latest developments and applications in a new and exciting field of spectroscopy - Fourier Transform Infra-Red (FTIR) spectroscopy.Table of ContentsTheoretical Analyses of the Amide I Infrared Bands of GlobularProteins (H. Torii & M. Tasumi). Fourier Transform Infrared Spectroscopy of Enzyme Systems (J.Alben). Light-Induced Fourier Transform Infrared Difference Spectroscopy ofthe Primary Electron Donor in Photosynthetic Reaction Centers (E.Nabedryk). Equipment: Slow and Fast Infrared Kinetic Studies (F.Siebert). Ultrafast Infrared Spectroscopy of Biomolecules (B. Cohen & R.Hochstrasser). Infrared Spectroscopy of Nucleic Acids (J. Liquier & E.Taillandier). Fourier Transform Infrared Spectroscopy in the Study of HydratedLipids and Lipid Bilayer Membranes (R. Lewis & R.McElhaney). Fourier Transform Infrared Spectroscopic Studies of Cell SurfacePolysaccharides (K. Brandenburg & U. Seydel). Fourier Transform Infrared Spectroscopy of Biomembrane Systems (P.Haris & D. Chapman). What Can Infrared Spectroscopy Tell Us About the Structure andComposition of Intact Bacterial Cells? (D. Naumann, et al.). Biomedical Infrared Spectroscopy (M. Jackson & H.Mantsch). New Trends in Isotope-Edited Infrared Spectroscopy (H. Fabian, etal.). Index.
£223.16
John Wiley & Sons Inc Quantitative Characterization of Ligand Binding
Book SynopsisThe book fills the void between rigorous theoretical discussions of ligand binding for physical chemists (often beyond the needs of bench experimentalists in the life sciences) and compilations of experimental recipes that offer scant regard for the appropriateness of the protocol for the particular ligand interaction under investigation.Table of ContentsIntroduction: The Analysis of Simple Binding Responses. Experimental Methods Based on Phase Separation. Spectral Studies of Ligand Binding. Other Direct Procedures for Characterizing Ligand Binding. Binding Constants from Competitive Binding Assays. Sigmoidal Binding Curves: Analysis and Interpretation. Complicating Factors in the Analysis of Binding Curves. Studies of DNA-Ligand Interactions. Concluding Remarks. Glossary of Symbols. References. Index.
£128.66
John Wiley & Sons Inc Molecular Dynamics Simulation
Book SynopsisProvides a lot of reading pleasure and many new insights. -Journal of Molecular Structure This is the most entertaining, stimulating and useful book which can be thoroughly recommended to anyone with an interest in computer simulation. -Contemporary Physics A very useful introduction . . . more interesting to read than the often dry equation-based texts. -Journal of the American Chemical Society Written especially for the novice, Molecular Dynamics Simulation demonstrates how molecular dynamics simulations work and how to perform them, focusing on how to devise a model for specific molecules and then how to simulate their movements using a computer. This book provides a collection of methods that until now have been scattered through the literature of the last 25 years. It reviews elements of sampling theory and discusses how modern notions of chaos and nonlinear dynamics explain the workings of molecular dynamics. Stresses easy-to-use molecules Table of ContentsFundamentals. Hard Spheres. Finite-Difference Methods. Soft Spheres. Static Properties. Dynamic Properties. Appendices. Notation. Bibliography. Index.
£140.35
John Wiley & Sons Inc Safety in the Chemistry and Biochemistry
Book SynopsisChemical and biochemical Laboratories are full of potentially dangerous chemicals and equipment. 'Safety in the Chemistry and Biochemistry Laboratory' provides the necessary information needed for working with these chemicals and apparatus to avoid: fires, explosions, toxic fumes, skin burns, poisoning and other hazards.Table of ContentsFrom the Contents: Chemical Risks: The Handling of Chemical Substances, Neutralization and Destruction of Chemical Substances/ Other Risks in the Chemistry Laboratory: Biological Risks, Laboratory Risks Associated with Non-Ionizing Radiation, Laboratory Risk Associated with Radioelements/ Bibliography/ Index/ Appendices
£160.16
John Wiley & Sons Inc Microbial Polyesters
Book SynopsisThis is the first book to present a topical overview of the research and development of microbial polyesters. Comprehensive and amply illustrated, it covers the * biosynthesis * structure * properties * applications of these biodegradable and biocompatible thermoplastics. The book provides the reader with the necessary background to understand the nature and mechanism of biological polymerization. It unites aspects from both the biological and polymer sciences and is a typical modern example of the analysis of biological macromolecules.Table of ContentsChapter 1 Introduction 1.1 Microbial Poly(3-hydroxybutyrate) 1.2 Microbial Poly(hydroxyalkanoates) 1.3 Environmentally Degradable Polyesters References Chapter 2 Fermentation and Analysis of Microbial Polyesters 2.1 Fermentation Production 2.1.1 Poly(3-hydroxybutyrate) 2.1.2 Poly(hydroxyalkanoates) 2.2 Polymer Isolation 2.2.1 Solvent Extraction 2.2.2 Alkaline Hypochlorite Treatment 2.2.3 Enzyme Treatment 2.3 Analysis 2.3.1 Polyester Content of Cells 2.3.2 Composition of Copolymers 2.3.3 Molecular Weight References Chapter 3 Microorganisms and Poly(3-hydroxyalkanoates) 3.1 Poly(3-hydroxybutyrate) in Microorganisms 3.1.1 Functions of Poly(3-hydroxybutyrate) 3.1.2 Structure of Native P(3HB) Granules 3.2 Biosynthesis of Poly(3-hydroxyalkanoates) 3.2.1 Alcaligenes eutrophus 3.2.2 Pseudomonas oleovorans 3.2.3 Other Bacterial Strains 3.3 Molecular Structures of Poly(3-hydroxyalkanoates) 3.3.1 Poly(3-hydroxybutyrate-co-3-hydroxyalerate) 3.3.2 Poly(3-hydroxyalkanoates-co-3-hydroxy--chloroalkanoates) References Chapter 4 Poly(3-hydroxyalkanoates) Metabolism 4.1 Pathways of Poly(3-hydroxybutyrate) Synthesis 4.2 Pathways of Poly(3-hydroxyalkanoates) Synthesis 4.3 Enzymology of Poly(3-hydroxyalkanoates) Synthesis 4.3.1 3-Ketothiolase 4.3.2 Acetoacetyl-CoA Reductase 4.3.3 P(3HB) Synthase 4.4 Pathways of P(3-hydroxybutyrate) Degradation 4.5 Cyclic Nature of Poly(3-hydroxyalkanoates) Metabolism 4.5.1 Replacement of P(3HB) by P(3HB-co-3HV) 4.5.2 Replacement of P(3HB-co-3HV) by P(3HB) 4.5.3 Application to PHA Fermentation References Chapter 5 Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) 5.1 Alcaligenes eutrophus and Carbon Substrates 5.2 Molecular Structure 5.3 Biosynthetic Pathway References Chapter 6 Structure and Properties of Poly(3-hydroxybutyrate) 6.1 Crystal Structure and Properties 6.1.1 Crystal Structure 6.1.2 Solid-State Properties 6.2 Solution Properties References Chapter 7 Solid-State Properties of Copolyesters 7.1 Composition and Physical Properties 7.1.1 X-Ray Diffraction Analysis 7.1.2 Solid-State CP/MAS 13C-NMR Analysis 7.1.3 Mechanical Properties 7.2 Thermal Properties 7.2.1 Melting Temperatures 7.2.2 Glass-Transition Temperatures 7.2.3 Thermal Stability 7.3 Kinetics of Crystallization References Chapter 8 Biodegradation of Microbial Polyesters 8.1 Extracellular P(3HB) Depolymerase 8.1.1 Pseudomonas lemoignei 8.1.2 Alcaligenes faecalis 8.2 Enzymatic Hydrolysis of Copolyesters 8.3 Simple Hydrolysis of Polyesters 8.4 Applications and Prospects 8.4.1 Environmentally Degradable Plastics 8.4.2 Medical Applications References Index
£177.26
John Wiley & Sons Inc Inorganic Biochemistry
Book SynopsisThe text will provide a set of problems covering mechanistic, structural and spectroscopic issues in inorganic chemistry. Specific areas to be covered include coordination chemistry, physiochemical aspects of solution chemistry, inorganic chemistry of biological systems (both natural biomolecules and bioinorganic models). Illustrative worked examples will be included. The problems will be categorized by topic chapters for ease of reference and use in courses. They will provide a valuable resource for instructors, providing a means of testing and developing the many principles covered in texts and advanced courses. Often students find it difficult to find practical problems to test the principles they have learned in class. This text will provide a series of questions to test understanding and worked examples as a pedagogical aid.Table of ContentsFundamentals of Inorganic Biochemistry. Experimental Methods. Transport and Storage. Metalloproteins and Metalloenzymes: (I) Oxygen Carriers and Hydrolases. Metalloproteins and Metalloenzymes: (II) Redox Chemistry. Alkali and Alkaline Earth Metals. Metals in the Regulation of Biochemical Events. Cell Toxicity and Chemotherapeutics. Metal Complexes as Probes of Structure and Reactivity. Case Studies. Appendices. Index.
£132.26
John Wiley & Sons Inc The Total Synthesis of Natural Products Volumes
Book SynopsisThese two volumes provide a comprehensive look into one of chemistry's fastest growing specialities - sesquiterpene synthesis. They provide a useful and practical tool for researchers interested in locating any of the major classes of sesquiterpene compounds.Table of ContentsAcknowledgments Introduction Reagent Glossary III. Bicyclic Sesquiterpenes A. Eudesmanes 1. β-Eudesmol, α-Eudesmol, Intermediol, Neointermediol, Amiteol, Cycloeudesmol, Maaliol, α-Selinene, β-Selinene, γ-Selinene, Selinadiene, AE 1, Vetiselinene 2. β-Dictyopterol, Selin-4(15)-ene-1β, 11-diol, Balanitol, Occidentalol, Isochamaecynone, Chamaecynone, Emmotin-G, Occidol 3. Amitermes Defensive Substance, Isocelorbicol 4. α-Cyperone, β-Cyperone, Carissone 5. Eudesma-4(14),7(11)-diene-8-one, Arctiol, β-Costol, β-Costal, Isocostic Acid, 3-Oxoisocostic Acid, 1-Oxocostic Acid 6. α-Costal, 7-Hydroxycostol, 7-Hydroxycostal 7. 3-Oxoeudesmatrienoic Acid, 12,15-Dioxaselina-4,11-diene 8. Junenol, Acolamone 9. Atractylon, Lindestrene, Tubipofuran 10. Glutinosone 11. Dehydroasterolide, Dihydrocallitrisin, Isoalantolactone, Septuplinolide, Ivalin, Oxodiplophyllin, Yomogin 12. Frullanolide, Tuberiferin, Deoxybrachylaenolide, Gazanolide, Arbusculin D, 3-Oxo-11-hydroxy-eudesmadienolide, Magnolialide, Maritimin, Dihydrosantamarine, α-Santonin B. Cadinanes 1. Calamenene, Hydroxycalamenenes, Methoxycalamenenes 2. Mansonones, Perezinone 3. 7,8-Dihydroxy-11,12-dehydrocalamenene, Heritol 4. Lacinilene C 5. α-Cadinene, γ2-Cadinene, ε-Cadinene, ε-Muurolene, β-Cadinene, δ-Cadinene 6. Zonarene, Dihydropernetic Acid B, Veticadinol, Torreyol, Isocalamenediol, Cubenol, Epicubenol 7. 1,4-Epoxycadinane, Verboccidentafuran, Hibiscone, Arteannuin B 8. Halipanicine, Sclerosporin, Sclerosporal, Khusilal C. Drimanes 1. Bicyclofarnesic Acid, Drimenol, Albicanyl Acetate, Albicanol, Farnesiferol A 2. Euryfuran, Valdiviolide, Confertifolin, Isodrimenin, Cinnamolide, Polygodial, Drimenin 3. Fragrolide, Cinnamodial, Warburganal, Isotadeonal, Muzigidial, Colorata-4(13),8-dienolide, 3β-Acetoxydrimenin, Pereniporin A, Pereniporin B 4. Driman-8,11-diol, Drim-9(11)-en-8-ol, 7-Oxo-11-nordrimen-8-en-12-oic Acid, 1,1,5,6-Tetramethyltetralin D. Eremophilanes 1. Nootkatone, Valencene, Isovalencenic Acid, Isovalencenol, Eremoligenol, Eremophilene, Valerianol, Eremophilone, Dehydrofukinone, Aristolone, Isopetasol, Petasol, Petasitolone 2. Phomenone, Eremofortin B, Sporogen-AO 1 3. Ligularenolide, Ligularone, Petasalbine, Eremophilenolide, Furanoeremophilone, Furanoeremophilan-14,6a-olide E. Miscellaneous Hydronaphthalenes 1. Acetyldehydrorishitinol, Cinalbicol, 4-Methoxyisocadalene, 4-Hydroxyisocadalene, 1-Hydroxyisocadal-4-one, 2-Methoxy-7-norcalamenene, Maturone, Platyphyllide 2. Ambrox, Pallescensin A 3. Furodysin, Furodysinin 4. Lemnal-5a-en-2-one, Kanshone A, Nardosinone 5. Valerane, Valeranone, Cryptofauronol, Fauronyl Acetate 6. Isoacanthodoral, Nanaiomoal 7. Thujopsene F. Fused Ring Compounds: 6,3 1. Sirenin, Sesquicarene, Isosesquicarene G. Fused Ring Compounds: 6,5 1. Pterosins 2. Pacifigorgiol, Tamariscol 3. Brasilenol, Conocephalenol 4. Axamide-1, Axisonitrile-1, Axamide-4, Axisonitrile-4, Axisothiocyanate-4, Oppositol 5. Pinguisone, Deoxopinguisone 6. Alliacolide 7. Chiloscyphone 8. Coriamyrtin, Asteromurin, Picrotoxinin, Picrotin 9. Oplopanone, Anhydrooplopanone 10. Bakkenolide A, Homogynolide A, Homogynolide B, Palmosalide C 11. Thapsane 12. Valerenal 13. 8-Deoxyanisatin, Anisatin, Neoanisatin, Noranisatin 14. Isovelleral, Stearoylvelutinal 15. Marmalerin H. Fused Ring Compounds: 6,7 1. ar-Himachalene, α-Himachalene, β-Himachalene, γ-Himachalene 2. Perforenone, Guadalupol, Epiguadalupol, Perforene 3. Widdrol 4. Pallescensin E, Pallescensin F, Pallescensin G I. Fused Ring Compounds: 6,8 1. Neolemnane, Neolemnanyl Acetate, Parvifolin J. Fused Ring Compounds: 5,7 1. Lettucenin A 2. β-Bulnesene, α-Bulnesol 3. Gnididione 4. Curcumenol, Curcumol 5. Liguloxide, Kessane 6. Alismol 7. Guaianolides: Compressanolide, Estafiatin 8. Guaianolides: Arborescin, 3-Epizaluzanin C, Zaluzanin C, Mokko Lactone, Dehydrocostus Lactone, Eremanthin 9. Guaianolides: Grosshemin, Oxoisodehydroleucodin, Jalcagauianolide 10. Pseudoguaianolides: Ambrosanolides-Confertin, Rudmollin 11. Pseudoguaianolides: Helananolides-Aromatin, Aromaticin, Graveolide, Fastigilin C, Carpesiolin 12. Daucene, Aspterric Acid 13. Mintsulfide, Aphanamol-I, 2-Oxoisodauc-5-en-12-al 14. Furanol, Furanether A, Furanether B, Furandiol, Lactarorufin A, Furoscrobiculin B 15. Africanol 16. Spathulenol, Alloaromadendrane-4α,10α-diol, Cyclocolorenone, Didehydroaromadendrane 17. Clavukerin A, Clavukerin C, Clavularin A and B K. Fused Ring Compounds: 5,8 1. Precapnelladiene 2. Poitediol 3. Dactylol 4. Asteriscanolide L. Fused Ring Compounds: 4,9 1. Isocaryophyllene, Caryophyllene 2. Punctaporonin B M. Fused Ring Compounds: 5,5 1. Pentalenolactone E 2. Pentalenolactone, Pentalenolactone G, Pentalenolactone H, Pentalenolactone P N. Isolated Rings 1. Cuparene, Herbertene, Tochuinol 2. α-Cuparenone, β-Cuparenone 3. Laurene, Epilaurene, Isolaurene 4. Allolaurinterol, Cyclolaurene 5. Aplysin, Filiformin, Aplysinol, Isoaplysin 6. Trichodiene, Bazzanene 7. Trichodermol, Verrucarol 8. Calonectrin, Anguidine 9. 12,13-Epoxytrichothec-9-ene, 15-Hydroxytrichothec-9,12-diene 10. Neosporol, Sporol 11. Dihydrofomannosin Acetate, Fomannosin O. Spirocyclic Systems 1. Acoradiene, α-Acoradiene, Acoratriene, β-Acorenol, β-Acoradiene 2. Acorone, Acorenone, Acorenone B 3. Hinesol, Agarospirol, α-Vetispirene, β-Vetispirene 4. β-Vetivone, Anhydro-β-rotunol 5. Solavetivone, 15-nor-Solavetivone, 3-Hydroxysolavetivone, Aglycone A3, Lubimin, Oxylubimin, Isolubimin, Lubiminol 6. Vitrenal 7. Spirolaurenone 8. Erythrodiene 9. Cinera-5,7,11-trien-9-one 10. α-Chamigrene, β-Chamigrene, Bromomethylenechamigrenone P. Bridged Systems 1. β-Santalol, β-Santalene, epi-β-Santalene, α-Santalol, α-Santalene 2. α-cis-Bergamotene, β-cis-Bergamotene, β-trans-Bergamotene, (E)-endo-α-Bergamoten-12-oic acid, (E)-endo-β-Bergamoten-12-oic acid, Pinthunamide 3. Helminthosporal 4. Upial 5. Nakafuran-8, Nakafuran-9, Dihydropallescensin 6. Dihydrospiniferin-1, Spiniferin-1 IV. Tricyclic Sesquiterpenes A. Angular Triquinanes 1. Isocomene 2. Silphinene, 8-Oxo-silphinene 3. Pentalenene, Pentalenic Acid 4. Silphiperfolenes, Methyl Cantabrenonate, Subergorgic Acid B. Propellanes 1. Modhephene C. Linear Triquinanes 1. Hirsutene 2. Capnellenes 3. Coriolin, Hypnophilin 4. Hirsutic Acid C, Cerotopicanol D. Miscellaneous Fused Tricyclic Sesquiterpenes 1. β-Bourbonene, Panasinsene, Italicene 2. Protoillud-6-ene, 8-Hydroxy-protoillud-6-ene, Illudol 3. Punctatin A, Ptaquilosin, Illudin M 4. Sterpuric Acid, Sterpurene-3,12,14-triol, Sterpurene E. Bridged Tricyclic Sesquiterpenes 1. Khusimone, Zizaene, Zizanoic acid, Prezizaene, Prezizanol, Jinkohol 2. Patchouli alcohol, Norpatchoulenol, Seychellene, Cycloseychellene 3. Longifolene, Culmorin, Longiborneol 4. Copacamphor, Ylangocamphor, Cyclosativene, Sativene, cis-Sativenediol, Sinularene, Acetoxysinularene 5. Quadrone 6. Cedrene, Isocedrenes, 8,14-Cedranoxide, 8,14-Cedranediol, Biotol, Pipitzols 7. α-Copaene, β-Copaene, β-Ylangene, Lemnalol, Longipinene 8. 9-Isocyanopupukeanane 9. Gymnomitrol 10. Secoishwaranol, Ishwarane 11. Rhodolauradiol, Khusiol Epilogue References Skeleton Index Author Index Subject Index
£409.46
John Wiley & Sons Inc Advances in Enzymology and Related Areas of
Book SynopsisEnzymes are the primary catalysts of life processes. This book covers the latest advances in enzymology, explaining the behaviour of enzymes and how they can be utilized to develop novel drugs, synthesize known and novel compounds, and understand evolutionary processes.Table of ContentsContributors. Preface. Abstracts. Molecular Paleoscience: Systems Biology from the Past (Steven A. Benner, Slim O. Sassi, and Eric A. Gaucher). A Structure-Centric View of Protein Evolution, Design, and Adaptation (Eric J. Deeds and Eugene I. Shakhnovich). Mechanisms of Protein Evolution and Their Application to Protein Engineering (Margaret E. Glasner, John A. Gerlt, and Patricia C. Babbitt). Novel Enzymes Through Design and Evolution (Kenneth J. Woycechowsky, Katherina Vamvaca, and Donald Hilvert). Author Index. Subject Index.
£152.95
John Wiley & Sons Inc Thermodynamics of Biochemical Reactions
Book SynopsisThermodynamics of Biochemical Reactions emphasizes the fundamental equations of thermodynamics and the application of these equations to systems of biochemical reactions. This emphasis leads to new thermodynamic potentials that provide criteria for spontaneous change and equilibrium under the conditions in a living cell.Trade Review"Through the writing of his new book, Professor Alberty has performed a great service to future textbook authors and to their readers, as well as to the biochemical community in general.” (Biochemistry and Molecular Education, Nov/Dec 2003) "...a useful learning device...through the writing of his new book, Professor Alberty has performed a great service to future textbook authors and their readers, as well as to the biochemical community in general." (Biochemistry and Molecular Biology Education,Vol. 31, No. 6) "...this book will prove useful for students and working biochemists...highly recommended..." (Choice, September 2003) "...emphasizes the application of the fundamental equations of thermodynamics to systems of enzyme-catalyzed reactions making up the metabolism of living organisms." (Pharmaceutical Research, Vol. 20, No. 9, September 2003) "...written by the most experienced authority in this field...well-written and -organized, and nicely produced..." (Angewandte Chemie International Edition, Vol 42(37), 2003) "...the authors are to be congratulated on producing an updated, valuable and comprehensive review of the field..." (ChemPhysChem, Vol 5, 2004)Table of ContentsPreface. Chapter 1. Introduction to Apparent Equilibrium Constants. Chapter 2. Structure of Thermodynamics. Chapter 3. Chemical Equilibrium in Aqueous Solutions. Chapter 4. Thermodynamics of Biochemical Reactions at Specified pH. Chapter 5. Matrices in Chemical and Biochemical Thermodynamics. Chapter 6. Systems of Biochemical Reactions. Chapter 7. Thermodynamics of Binding of the Ligands by Proteins. Chapter 8. Phase Equilibrium in Aqueous Systems. Chapter 9. Oxidation-Reduction Reactions. Chapter 10. Calorimetry of Biochemical Reactions. Chapter 11. Use of Semigrand Partition Functions. Glossary. References.
£112.46
John Wiley & Sons Inc Bioseparations Engineering Principles Practice
Book SynopsisBioseparations engineering is the "multidisciplinary application of fundamental engineering and biological principles to the design of adsorbents, systems, and processes for the separation of biological molecules.Trade Review"a comprehensive text that has brought together the theory and practice of bioseperations in an intelligent and well-presented fashion." (Bioseperations Engineering (Food & Bioproducts Processing, December 2001) "...Ladish reviews the techniques that have been developed over the past couple decades...in the hope that the explanations will apply to bioproducts not yet invented and biological molecules not yet produced...as well as to those currently being used." (SciTech Book News, Vol. 25, No. 4, December 2001)Table of ContentsPreface. Acknowledgements. Bioseparations. Sedimentation, Centrifugation, and Filtration. Membrane Separations. Precipitation, Crystallization, and Extraction. Principles of Liquid Chromatography. Liquid Chromatography Scale-Up. Principles of Gradient Elution Chromatography. Principles of Bioseparations for Biopharmaceuticals and Recombinant Protein Products. Affinity Chromatography: Bridge Between Molecular Biology, Combinatorial Methods, and Separations Science. Author Index. Subject Index.
£161.95
John Wiley & Sons Inc Advances in Enzymology and Related Areas of
Book SynopsisBiological catalysis plays a dominant role both in fermentation and industrial process chemistry. This collection of chapters, written by a well-known biochemist and enzymologist, should serve as an invaluable reference to those investigators seeking to optimize the application of enzymatic catalysis for commercial purposes.Table of ContentsClassics in Enzymology: The Kinetics of the Enzyme-SubstrateCompound of Peroxidase (B. Chance). Rethinking Fundamentals of Enzyme Action (D. Northrop). Adenylosuccinate Synthetase: Recent Developments (R. Honzatko, etal.). Nucleoside Monophosphate Kinases: Structure, Mechanism, andSubstrate Specificity (H. Yan & M.-D. Tsai). Enzymology of NAD?+ Synthesis (G. Magni, et al.). Solution Structure and Mechanism of MutT Pyrophosphohydrolase ( A.Mildvan, et al.). The Enzymes of Glutathione Synthesis: gamma-GlutamylcysteineSynthetase (O. Griffith & R. Mulcahy). Indexes.
£250.16
John Wiley & Sons Inc StructureFunction Analysis of G ProteinCoupled
Book SynopsisG protein-coupled receptors (GPCRs) are the largest single class of receptors in biology, often playing key roles in a remarkably large number of physiological and pathophysiological conditions. GPCRs or GPCR-dependent signalling pathways are the targets of a very large number of therapeutically useful drugs. Detailed knowledge about the molecular structure of GPCRs should therefore pave the way for the design of novel drugs with increased efficacy and specificity. This volume provides a concise, up-to-date presentation of methods (including molecular genetic, biochemical, and biophysical) which have been used successfully in studying the structure and function of GPCRs. With contributions from international leaders in the field, the editor provides overviews of various techniques, followed by in-depth descriptions of basic procedures and discussions of critical experimental parameters. Divided into specific, accessible sections, Structure-Function Analysis of G ProteTable of ContentsPartial table of contents: Overview of Mutagenesis Techniques (T. Fong). The Substituted-Cysteine Accessibility Method (J. Javitch). Metal-Ions as Atomic Scale Probes of G Protein-Coupled Receptor Structure (J. Schetz & D. Sibley). Genetic Approaches for Studying the Structure and Function of G Protein-Coupled Receptors in Yeast (C. Sommers & M. Dumont). Electron-Crystallographic Analysis of Two-Dimensional Rhodopsin Crystals (G. Schertler). Site-Directed Spin-Labeling (SDSL) Studies of the G Protein-Coupled Receptor Rhodopsin (D. Farrens). Lead Discovery and Development for G Protein-Coupled Receptors (D. Underwood & M. Cascieri). Index.
£276.26
John Wiley & Sons Inc Chemistry and Biochemistry of B12
Book SynopsisThere have been dramatic advances in the field of researching Vitamin B12, and those advances have served to revitalize interest in Vitamin B12 among biochemists, organic and inorganic chemists, microbiologists, structural biologists, and many other science professions.Table of ContentsCHEMISTRY OF B_12. B_12 History (H. Hogenkamp). X-ray Crystallography of B_12 (B. Kräutler & C. Kratky). X-Ray Absorption Spectorscopy of B_12: Structural Changes of Intermediate States (M. Chance). Electronic Structure and Spectra of B_12: From Trans Effects to Protein Conformation I (J. Pratt). Electronic Structure and Spectra of B_12: From Trans Effects to Protein Conformation II (J. Pratt). EPR Spectroscopy of B_12-Dependent Enzymes (G. Gerfen). NMR Spectroscopy of B_12 (K. Brown). Vibrational Spectroscopy of B_12 and Related Compounds (L. Marzilli & S. Hirota). Magnetic Field Dependence of Cobalamin Photochemistry and Enzymes (C. Grissom). Stereospecificity of the Coenzyme B_12 Catalyzed Rearrangements and the Role of Negative Catalysis (J. Retey). Modeling the Structure of Cobalt Corrins by Molecular Mechanics and Molecular Dynamics Methods (H. Marques). B_12 Electrochemistry and Organometallic Electrochemical Synthesis (B. Kräutler). BIOCHEMISTRY AND BIOLOGY OF B_12. B_12 and Nutrition (S. Stabler). Inborn Errors of Cobalamin Metabolism (D. Rosenblatt & W. Fenton). Diagnostic and Therapeutic Analogs of Cobalamin (H. Hogenkamp, et al.). Intrinsic Factor and Haptocorrin and Their Receptors (D. Alpers & G. Russell-Jones). Transcobalamin II (S. Rothenberg, et al.). Mammalian Receptors of Vitamin B_12-Binding Proteins (S. Moestrup & P. Verroust). Cobalamin Transport in Bacteria (C. Bradbeer). Biosynthesis of B_12 in the Aerobic Organism Pseudomonas denitrificans (A. Battersby & F. Leeper). B_12 Biosynthesis: The Anaerobic Pathway (A. Scott, et al.). Biosynthesis of the 5-6 Dimethylbenzimidazole Moiety of Cobalamin and of the Other Bases Found in Natural Corrinoids (P. Renz). Regulation of Adenosylcobalamin Biosynthesis in Salmonella typhimurium (J. Semerena). X-ray Crystallography of B_12 Enzymes: Methylmalonyl-CoA Mutase and Methionine Synthase (M. Ludwig & P. Evans). The Acetogenic Corriniod Proteins (S. Ragsdale). The Role of Corrinoids in Methanogenesis (K. Sauer & R. Thauer). Methionine Synthase (R. Matthews). Methylmalonyl-CoA Mutase (R. Banerjee & S. Chowdhury). Ribonucleotide Reductases (M. Fontecave & E. Mulliez). Glutamate Mutase and 2-Methyleneglutarate Mutase (W. Buckel, et al.). Diol Dehydrase and Glycerol Dehydrase (T. Toraya). Ethanolamine Ammonia-Lyase (V. Bandarian & G. Reed). Anomutases (P. Frey). Isobutyryl-CoA Mutase (K. Zerbe-Burkhardt, et al.). Reductive Dehalogenases (G. Wohlfarth & G. Diekert).
£402.26
John Wiley & Sons Inc Basics of Toxicology Preserving the Legacy
Book SynopsisToxicology--the scientific study of environmental factors that are harmful to living organisms--was established more than 400 years ago by the Swiss physician Paracelsus.Table of ContentsIntroduction to Toxicology. Epidemiological and Toxicological Studies. Routes of Exposure. The Dose-Response Relationship. Absorption, Distribution, Metabolism, and Elimination of Toxics. Target Organ Effects. Survey of Toxic Substances. Radiation, Pathogens, and Naturally Occurring Toxins. Mutagens, Teratogens, and Carcinogens. Risk Assessment and Acute Exposure Treatment. Glossary. Bibliography. Index.
£108.86
John Wiley & Sons Inc Tandem Mass Spectrometry 2 Wiley Series on Mass
Book SynopsisThis volume describes the complete process of analyzing protein sequences by mass spectrometry including sample preparation, analysis, and data interpretation at a practical level. It provides sufficient experimental detail to carry out the experiments and consistently achieve good results.Trade Review"[the authors] present their own laboratory-tested protocols for the analysis of a wide variety of samples, providing sufficient detail for duplication of their methods..." (SciTech Book News Vol. 25, No. 2 June 2001) "...an informative, well-written and well-illustrated book with a large number of useful references.... It should prove invaluable to anyone working in the field of protein sequencing." (Spectroscopyeurope.com)Table of ContentsAn Introduction to Protein Sequencing Using Tandem Mass Spectrometry. The Primary Structure of Proteins and a Historical Overview of Protein Sequencing. Fundamental Mass Spectrometry. Collisionally Induced Dissociation of Protonated Peptide Ions and the Interpretation of Product Ion Spectra. Basic Polyacrylamide Gel Electrophoresis. The Preparation of Protein Digests for Mass Spectrometric Sequencing Experiments. Mass Spectrometric Analysis of Tryptic Digests. Protein Identification by Database Searching. Sequence Analysis of Novel Proteins. The Characterization of Post-Translationally Modified Proteins Using Tandem Mass Spectrometry. Index.
£147.56
John Wiley & Sons Inc Air Pollution and Plant Life
Book SynopsisProvides a comprehensive overview of direct and indirect effects of air pollutants on plant life. This book covers both physiological and biochemical impacts as well as effects at the whole plant/ecological level.Trade Review"...should be studied by many, whose interest lies in the preservation of air quality..." (International Journal of Environment Studies, Vol.60, No.2, 2003) "...Bell and Treshow have produced what I suspect will be a classic. (Times Higher Educational Supplement, 30 May 2003)Table of ContentsIntroduction (Nigel Bell & Michael Treshaw) Historical Perspective (Michael Treshaw & Nigel Bell) Emissions, Dispersion, Atmospheric Transformation (Roy Colville) Deposition and Uptake by Vegetation (David Fowler) Effects of Oxidants at the Biochemical, Cell and Physiological Levels with Particular Reference to Ozone (Stephen Long and Shawna Naidu) Effects of Oxidants at the Whole Plant and Community level (Michael Ashmore) Nitrogen Oxides: Old Problems and new Challenges (Terry Mansfield) Effects of Sulphur Dioxide (Allan Legge & Sagar Krupa) Effects of Fluorides (Len Weinstein & Del McCune) Effects of Volatile Organic Compounds (Christopher Collins & Nigel Bell) Effects of Particulates (Andrew Farmer) Effects of Increased Nitrogen Deposition (Roland Bobbink & Leon Laners) Effects of Wet Deposited Acidity (Trevor Ashenden) Effects of Pollutant Mixtures (Andreas Fangmeier, Juergen Bender, Hans-Joachim Weigel & Hans-Juergen Jager) Forest Decline and Air Pollution: An Assessment of "Forest Health" in the Forests of Europe, the Northeastern United States and Southeastern Canada (John Innes & John Skelly) Effects of Acidic Deposition on Aquatic Ecosystems (Ronald Harriman, Richard Batterbee & Don Monteith) Effects on Bryophytes and Lichens (Jeffrey Bates) Modification of Plant Response by Environmental Conditions (Gina Mills) Air Pollutant - Abiotic Stress Interactions (Alan Davison & Jeremy Barnes) Effects of Air pollutants on Biotic Stresses (Walter Fluckiger, Sabine Braun & Erika Hiltbrunner) Effects of Air Pollutants in Developing Countries (Fiona Marshall) Air Quality Guidelines and their Role in Pollution Control Policy (Michael Ashmore) Air Pollution and Climate Change (Victor Runeckles) Future Research Priorities and Directions (Nigel Bell)
£201.56
John Wiley & Sons Inc Air Pollution Plant Life Second Edition
Book SynopsisProvides a comprehensive overview of the direct and indirect effects of air pollutants on plant life. This book also covers both physiological and biochemical impacts as well as larger-scale effects at the whole plant/ecological level. It is useful for students in Environmental Science, and Biological Science and Agriculture.Trade Review"...should be studied by many, whose interest lies in the preservation of air quality..." (International Journal of Environment Studies, Vol.60, No.2, 2003) "...Bell and Treshow have produced what I suspect will be a classic. (Times Higher Educational Supplement, 30 May 2003)Table of ContentsIntroduction (Nigel Bell & Michael Treshaw) Historical Perspectives (Michael Treshaw & Nigel Bell) Emissions, Dispersion and Atmospheric Transformation (Roy Colville) Deposition and Uptake by Vegetation (David Fowler) Effects of Oxidants at the Biochemical, Cell and Physiological Levels with Particular Reference to Ozone (Stephen Long and Shawna Naidu) Effects of Oxidants at the Whole Plant and Community Level (Michael Ashmore) Nitrogen Oxides: Old Problems and New Challenges (Terry Mansfield) Effects of Sulphur Dioxide (Allan Legge & Sagar Krupa) Effects of Fluorides (Len Weinstein & Del McCune) Effects of Volatile Organic Compounds (Christopher Collins & Nigel Bell) Effects of Particulates (Andrew Farmer) Effects of Increased Nitrogen Deposition (Roland Bobbink & Leon Laners) Effects of Wet Deposited Acidity (Trevor Ashenden) Effects of Pollutant mixtures (Andreas Fangmeier, Juergen Bender, Hand-Joachim Weigel & Hans-Juergen Jager) Forest Decline and Air Pollution: An Assessment of "Forest Health" in the Forests of Europe, the Northeastern United States and Southeastern Canada (John Innes & John Skelly) Effects of Acidic Deposition on Aquatic Ecosystems (Ronald Harriman, Ricahrd Battarbee & Don Monteith) Effects on Bryophytes and Lichens (Jeffrey Bates) Modifications of Plant Response by Environmental Conditions (Gina Mills) Air Pollutant - Abiotic Stress Interactions (Alan Davison & Jeremy Barnes) Effects of Air Pollutants on Biotic Stresses (Walter Fluckiger, Sabine Braun & Erika Hiltbrunner) Effects of Air Pollutants in Developing Countries (Fiona Marshall) Air Quality Guidelines and their Role in Pollution Control Policy (Michael Ashmore) Air Pollution and Climate Change (Victor Runeckles) Future Research Priorities and Directions (Nigel Bell)
£74.66
John Wiley & Sons Inc Affinity Membranes
Book SynopsisExplores the latest findings for both selective and efficient separation devices in the field of kidney research. It is divided into three major sections. Part one deals with the ``biochemistry'''' part of the problem, including how to identify ligands of interest, how to link them to synthetic membranes, and some kinetic limitations of frontal elution chromatography. The second part comprehensively discusses the various substrata used in affinity separations and the formation processes of semi-permeable membranes. The final section explores the filtration processes using membranes and the kinetics of separations based on affinity membranes.Table of ContentsAffinity Adsorption. Substrates. Binding Chemistries. Ligand Types. Capacity of Affinity Matrices. Membrane Formation. Characterization. Microfiltration. Applications for Affinity Microfiltration Membranes. Appendix. Index.
£239.36
John Wiley & Sons Inc Concepts in Biochemistry
Book SynopsisA comprehensive, up-to-date treatment of the biochemistry essential for an understanding of molecular and cellular biological processes. This third edition offers new units covering the chemistry of life, bioenergetics, energy transfer molecules, regulation of enzymes and reaction sequences, lab techniques for purification of proteins and nucleic acids, and lab techniques of molecular genetics. Also, each unit contains more applications to biological systems. The text provides a well-organized and rigorous approach suitable for classroom use or self-instruction. Each unit begins with a 1- to 2-page presentation of basic concepts, followed by about 20 questions and problems with sample responses. Self-tests appear after every 2 to 3 units and there is a cumulative self-test at the end of the book.Table of ContentsIntroduction to the Chemistry of Life. Particles, Atoms, and Molecules. Chemical Groups. Ions, Ionic Bonds, and Crystals. Polarity, Hydrogen Bonds, Solubility, and HydrophobicInteractions. Chemical Equilibrium. Carbohydrates I: Monosaccharides and Related Molecules. Carbohydrates II: Disaccharides, and Polysaccharides. Amino Acids. Proteins I: Peptide Bonding and Polypeptides. Proteins II: Levels of Structure and Conjugated Proteins. Lipids: Fats, Phospholipids, Sterols, and Prostaglandins. Nucleic Acids. Bioenergetics. Energy Transfer Molecules. Enzymes. Regulation of Enzymes and Reaction Sequences. Molecular Weight and Molar, Percent, Mg Percent, and ppmSolutions. pH and H?+ Concentration. Weak Acids, Dissociation Constants, and Buffers. Self-Test. Laboratory Methods. Cumulative Self-Test. Index.
£94.95
John Wiley & Sons Inc Proteomic Applications in Cancer Detection and
Book SynopsisBridging the knowledge gap between scientists that develop and apply proteomics technologies and oncologists who focus on understanding the biological basis behind cancer manifestation and progression, this title provides an account of how the multiple facets of proteomics have been applied to cancer.Trade Review“By guiding readers through the latest proteomic technologies and their applications in cancer research, “Proteomic Applications in Cancer Detection and Discovery" enhances the ability of researchers in proteomics and researchers in oncology to collaborate in order to better understand cancer and develop strategies to prevent and treat it.” (Newbooks.lib, 11 September 2014Table of ContentsPreface vii Acknowledgments ix 1 Systems Biology 1 2 Mass Spectrometry in Cancer Research 27 3 Quantitative Proteomics 59 4 Proteomic Analysis of Posttranslational Modifications 87 5 Characterization of Protein Complexes 117 6 Global Phosphorylation Analysis 145 7 the Search for Biomarkers in Biofluids 171 8 Proteomic Patterns: a New Paradigm in Diagnostics And Therapeutics? 195 9 the Emergence of Protein Arrays 215 10 the Role of Proteomics in Personalized Medicine 241 11 the Critical Role of Bioinformatics 263 12 Future Prospects of Proteomics in Cancer Research 281 Index 305
£71.06
John Wiley & Sons Inc Drug Metabolism Basic Concepts and Practice
Book SynopsisDrug Metabolism in Drug Design and Development provides a practical description of what happens during the drug design and development process and focuses on issues such as: what data are needed; what experiments and analytical methods are typically employed; and how to interpret and apply data.Trade Review"I highly recommend this book to anyone interested in drug metabolism." (Journal of Medicinal Chemistry, 2008)Table of ContentsPreface. Contributors. Part I: Basic Concepts of Drug Metabolism. 1. Overview: Drug Metabolism in the Modern Pharmaceutical Industry (Scott J. Grossman) 2. Oxidative, Reductive, and Hydrolytic Metabolism of Drugs (F. Peter Guengerich) 3. Conjugative Metabolism of Drugs (Rory Remmel, Swati Nagar and Upendra Argikar) 4. Enzyme Kinetics (Timothy S. Tracy) 5. Metabolism-Mediated Drug-Drug Interactions (Hongjian Zhang, Michael W. Sinz, and A. David Rodrigues) 6. Drug Transporters in Drug Disposition, Drug Interactions, and Drug Resistance (Cindy Q. Xia, Johnny J. Yang, and Suresh K. balani) 7. Regulatory Considerations of Drug Metabolism and Drug Interaction Studies (Xiaoxiong Wei and Mingshe Zhu) Part II: Role of Drug Metabolism in the Pharmaceutical Industry. 8. Drug Metabolism Research as an Integral Part of the Drug Discovery Process (W. Griffith Humphreys) 9. Role of Drug Metabosim in Drug Development (Ramaswamy Iyer and Donglu Zhang) Part III: Analytical techniques in Drug Metabolism. 10. Applications of Liquid Radiochromatography Techniques in Drug Metabolism Studies (Mingshe Zhu, Weiping Zhao, and W. Griffith Humphreys) 11. Application of Liquid Chromatogrpahy/Mass Spectrometry for Metabolite Identification (Shuguang Ma and Swapan K. Chowdhury) 12. Introductin to NMR and Its Application in Metabolite Structure Determination (Xiaohua Huang, Robert Powers, Adrienne Tymiak, Robert Espina, and Vikram Roongta) Part IV: Common Experimental Approaches and Protocols. 13. Determination of Metabolic Rats and Enzyme Kinetics (Zhi-Yi Zhang and Laurence S. Kaminsky) 14. Protocols for Assessment of In vitro and In vivo Bioactivation Potential of Drug Candidates (Zhoupeng Zhang and Jinping Gan) 15. Reaction Phenotyping (Susan Hurst, J. Andrew Williams, and Steven Hansel) 16. Analysis of In vitro Cytochrome P450 Inhibition in Drug Discovery and Development (Magang Shou and Renke Dai) 17. Testing Drug Candidates for CYP3A4 Induction (Gang Luo, Liang-Shang Gan, and Thomas M. Guenthner) 18. ADME Studies in Animals and Humans: Experimental Design, Metabolite Profoling and Identification, and Data Presentation (Donglu Zhang and S. Nilgun Comezoglu) Index.
£128.66
John Wiley & Sons Inc Introduction to Proteomics
Book SynopsisProteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases.Trade Review"The Author offers an expert perspective on the entire field, including: Easily accessible overview of the principles of proteomics; Coverage of real-world, cutting-edge medical applications, including personalized medicine; Clear guidelines on how to operate the complex instrumentation involved in proteomics; Discussion of the future of proteomics". (Anticancer Research, 1 August 2010) This book provides a highly authoritative introduction to the promising and fast-advancing field of proteomics, examining the role proteomics plays in the study of biological systems in general and disease in particular. It helps readers understand the structure, function, and interactions of proteins and how this knowledge is used for identifying diseases and developing new drugs." (Forbes.com, 1 November 2010) "For undergraduate and graduate biology and non-biology students and new researchers, Mishra (molecular genetics and genomics, U. of South Carolina) introduces the field of proteomics and its role in the study of biological systems and disease." (Book News, September 2010)Table of ContentsFOREWORD. PREFACE. ABOUT THE AUTHOR. CHAPTER 1 HISTORICAL PERSPECTIVES. 1.1. Introduction to Proteomics. 1.2. Proteome and Proteomics. 1.3. Genetics of Proteins. 1.4. Molecular Biology of Genes and Proteins. 1.5. Protein Chemistry Before Proteomics. References. Further Reading. CHAPTER 2 PROTEOMICS—RELATION TO GENOMICS, BIOINFORMATICS. 2.1. Genomics. 2.2. Bioinformatics and Computational Biology. References. Further Reading. CHAPTER 3 METHODOLOGY FOR SEPARATION AND IDENTIFICATION OF PROTEINS AND THEIR INTERACTIONS. 3.1. Separation of Proteins Via a Multidimensional Approach. 3.2. Determination of the Primary Structure of Proteins. 3.3. Determination of the 3D Structure of a Protein. 3.4. Determination of the Amount of Proteins. 3.5. Structural and Functional Proteomics. References. Further Reading. CHAPTER 4 PROTEOMICS OF PROTEIN MODIFICATIONS. 4.1. Phosphorylation and Phosphoproteomics. 4.2. Glycosylation and Glycoproteomics. 4.3. Ubiquitination and Ubiquitinomics. 4.4. Miscellaneous Modifications of Proteins. References. Further Reading. CHAPTER 5 PROTEOMICS OF PROTEIN–PROTEIN INTERACTIONS/INTERACTOMES. 5.1. Protein—Protein Interactions (PPI) in Vivo. 5.2. Analysis of Protein Interactions in Vitro. 5.3. Analysis of Protein Interactions in Silico. 5.4. Synthetic Genetic Methods to Determine Protein Interactions. 5.5. Interactomes. 5.6. Evolution and Conservation of Interactomes. 5.7. Interactomes and the Complexity of Organisms: It is the Number of Interactomes that Matters in Understanding the Complexity of an Organism and not the Number of Genes. 5.8. Interaction of Proteins with Small Molecules. References. Further Reading. CHAPTER 6 APPLICATIONS OF PROTEOMICS I: PROTEOMICS, HUMAN DISEASE, AND MEDICINE. 6.1. Diseasome. 6.2. Medical Proteomics. 6.3. Clinical Proteomics. 6.4. Metaproteomics and Human Health. 6.5. Proteomics in Biotechnology and Industry of Drug Production. 6.6. Metaproteomics of Microbial Fermentation. 6.7. Beef Industry. 6.8. Bioterrorism and Biodefense. References. Further Reading. CHAPTER 7 PROTEOMICS—FUTURE DEVELOPMENTS. 7.1. Technical Scope of Proteomics—Beyond Protein Identification. 7.2. Scientific Scope of Proteomics—Control of Epigenesis. 7.3. Medical Scope of Proteomics. 7.4. Proteomics, Energy Production, and Bioremediation. 7.5. Proteomics and Biodefense. References. Further Reading. INDEX.
£90.86
John Wiley & Sons Inc Redox Biochemistry
Book SynopsisRedox Biochemistry provides an excellent text and useful resource for students and practicing researches on redox biochemistry in medical biochemistry, enzyme structure and catalysis, environmental biochemistry, and nutrition.Table of ContentsContributors. List of Abbreviations. Preface. 1. Redox Metabolism and Life (Ruma Banerjee) 2. Antioxidant Molecules and Redox Cofactors (Donald Becker) 3. Antioxidant Enzymes (Vadim Gladyshev) 4. Redox Regulation of Physiological Processes (Martin Dickman) 5. Pathological Processes Related to Redox (Ruma Banerjee) 6. Specialized Methods (Stephen Ragsdale) 7. Index.
£121.46
John Wiley & Sons Inc FluorineContaining Amino Acids
Book SynopsisIn recent years, organo-fluorine chemistry has made a marked impacton the design and synthesis of a large variety of biologicallyactive molecules, such as steroids, carbohydrates, amines, aminoacids, peptides and other natural products. Naturally occurringamino acids play a pivotal role in living systems, and thereforesynthetic fluorine-containing amino acids have been of significantinterest to researchers working towards the understanding andmodification of physiological processes. Fluorine-containing AminoAcids: * is the first volume devoted to the synthesis and properties offluorine-containing amino acids * pays special attention to the preparation of enantiomericallypure acids (which are essential to the modern pharmaceuticalindustry) * deals with a rapidly expanding field of research * has been written by experienced researchers who are responsiblefor many developments in the field * highlights the interdisciplinary nature of this topic Fluorine-containing Table of ContentsPartial table of contents: Syntheses of Fluorine-Containing Amino Acids by Methods ofClassical Amino Acid Chemistry (V. Tolman). Preparation of Fluorine-Containing Amino Acids by Methods ofOrganofluorine Chemistry (V. Kukhar). Synthesis of Fluorine-Containing Amino Acids by Means ofHomogeneous Catalysis (I. Ojima & Q. Dong). Synthesis of beta-Fluorine-Containing Amino Acids (N. Sewald &K. Burger). Asymmetric Synthesis of Fluorine-Containing Amino Acids (V. Kukhar,et. al). Enzymatic Synthesis of Fluorine-Containing Amino Acids (Y.Matsumura & M. Urushihara). Enzymatic Resolution of Racemic Fluorine-Containing Amino Acids (T.Miyazawa). High-Performance Liquid Chromatography of Fluorine-Containing AminoAcids (S. Galushko). Renin Inhibitors with Fluorine-Containing Amino Acid Derivatives(H. Sham). Index.
£612.86
John Wiley & Sons Inc Immunoassays Essential Data 11 Essential Data
Book SynopsisImmunoassays are in use in many laboratories and across many disciplines. They involve the use of antibodies to detect and quantitate proteins. The Essential Data Series is a series of handy pocket-sized books which allow researchers to have all the key experimental facts at their fingertips.Table of ContentsHistory and Classification (R. Edwards). Antibodies and Antisera (R. Edwards). Assay Design (R. Edwards). Seperation (R. Edwards). Radiolabeled Immunoassays (R. Edwards). Enzyme-labeled Immunoassay (J. Little). Flurescent and Phosphorescent Labeled Assays (S. Blincko). Chemiluminescent and Bioluminescent Labeled Assays (S. Blincko). Precipitation and Agglutination Methods (J. Little). Dry Surface Immunoassays and Immunosensors (R. Edwards). Data Processing (I. Howes). Manufacturers and Suppliers. References. Index.
£77.36
John Wiley & Sons Inc Progress in Pesticide Biochemistry and Toxicology
Book SynopsisThis study explores the movement of pesticides into ground water, describing how computers can be used to model this phenomenon. It features novel approaches to the analysis of environmental samples and reviews analytical methods for the detection of agrochemical run-off.Table of ContentsEstimation of Physicochemical Properties: Theoretical andExperimental Approaches (P. Fisk). Recommended Approaches to Assess Pesticide Mobility in Soil (M.Russell). Soils and Pesticide Mobility (C. Brown, et al.). Pesticide Leaching Models and Their Use for Management Purposes (N.Jarvis, et al.). Pesticides in Run-Off and Surface Waters (B. Burgoa & R.Wauchope). The Volatization of Pesticide Residues (A. Taylor). Biosensors for Detection of Pesticides (M. Eldefrawi, etal.). Techniques and Procedures for the Determination of Pesticides inWater (C. Eadsforth & A. Woodbridge). Index.
£417.56
John Wiley & Sons Inc The Methyl Bromide Issue
Book SynopsisWritten by international experts in the field, this book sets out the scientific arguments for and against the use of methyl bromide which is both naturally occurring and also a man-made product believed to have played a significant role in the depletion of the ozone layer.Trade Review"The four chapters covering the chemistry and mammalian toxicology of the compound, its effects on target organisms, uses in storage practice, uses in storage practice, and alternative method sof preventing infestation and spoilage are written by scientists with established reputations in the fields of fumigation and food storage practice from this institution. What makes the book particularly useful is the juxtaposition of these chapters with contributions from leading experts in atmospheric ozone depletion and environmental analysis (in both the chemical and political sense)." Pesticide ScienceTable of ContentsMethyl Bromide in Perspective (N. Price). Methyl Bromide in the Atmosphere (J. Butler & J.Rodriguez). Methyl Bromide and the Environment (M. Miller). Effects on Target Organisms (O. Macdonald & C.Reichmuth). Methyl Bromide as a Soil Fumigant (L. Klein). Methyl Bromide in Storage Practice and Quarantine (B.Chakrabarti). Alternatives--Chemicals (P. Annis & C. Waterford). Alternatives--Physical Methods and Emission Reduction (C.Bell). Indexes.
£190.76
John Wiley & Sons Inc Metal Speciation and Bioavailability in Aquatic
Book SynopsisMetal Speciation and Bioavailability in Aquatic Systems is thefirst comprehensive review to deal with fundamental concepts andmodels, speciation measurements and field applications in metalspeciation and bioavailability in aquatic environments. This volumeprovides a thorough review of current developments concerning theinteractions between trace metals and aquatic organisms. MetalSpeciation and Bioavailability in Aquatic Systems provides: * The first comprehensive approach to the subject covering allaspects of trace metal ecotoxicology in the environment. * Essential reading for researchers and graduate students who willappreciate critical reviews of classical and modern methods ofmetal speciation. * Contributions from leading scientists from many disciplinesassessing both analytical and physical methods applied to thisgrowing field of environmental analysis. * An introduction to the use of laboratory bioassays as predictivetools for understanding trace metal organism iTable of ContentsTransport of Metals Across Membranes (K. Simkiss & M.Taylor). Interactions between Trace Metals and Aquatic Organisms: A Critiqueof the Free-Ion Activity Model (P. Campbell). Environmental Organometallic Chemistry of Mercury, Tin and Lead:Present Status and Perspectives (E. Pelletier). Problems in Trace Metal Speciation Modeling (D. Turner). Trace Metal Speciation of Labile Chemical Species in Natural Water:Electrochemical Methods (A. Mota & M. Correia dosSantos). Trace Metal Speciation of Labile Chemical Species in Natural Watersand Sediments: Non-Electrochemical Approaches (S. Apter & G.Batley). Chromatographic Approaches to Trace Element Speciation ofNon-Labile Chemical Species (W. Marshall & G.-M. Momplaisir). The Trophic Transfer of Metals in Marine Systems (N. Fisher &J. Reinfelder). Biological Factors Involved in Metal Concentrations Observed inAquatic Organisms (W. Langston & S. Spence). Metal Detoxification in Aquatic Organisms (A. Mason & K.Jenkins). Prediction of Metal Toxicity in Nature from Bioassays: Limitationsand Research Needs (S. Luoma). Index.
£670.46
John Wiley & Sons Inc Practical Organic Mass Spectrometry
Book SynopsisPractical Organic Mass Spectrometry Second Edition A Guide for Chemical and Biochemical Analysis J. R. Chapman Kratos Analytical Instruments, Manchester, UK This volume provides a comprehensive survey of current techniques for the use of mass spectrometry in organic chemical and biochemical analysis. Every aspect of modern instrumentation and technique is discussed. The new edition retains the effective division of material applied in the author''s previous volume-theory, practical requirements and applications. However, it has been thoroughly revised and extended to include all recent advances in mass spectrometry, and is complete with extensive references. This is essentially a book for the practising mass spectroscopist which will appeal to both biochemists and organic chemists. Some familiarity with basic principles is assumed but the author has employed a style which makes this volume suitable for beginners and more advanced students alike. The present volume will be particularly Table of ContentsInstrumentation. Sample Introduction. Chemical Ionization: Ion-Molecule Reactions. Negative Ion Chemical Ionization. The Ionization of Labile Materials I. The Ionization of Labile Materials II. Tandem Mass Spectrometry: The Dissociation of Ions. Quantitative Analysis. Appendices. Index.
£138.56
John Wiley & Sons Inc Transgenic Plants
Book SynopsisTransgenic Plants: A Production System for Industrial andPharmaceutical Proteins provides a detailed guide to the principlesand practice of using transgenic plants as a system for theproduction of heterologous proteins. It is unique in that it coversthe complete process of heterologous protein production in plants,from the initial transformation of the plant, through totranscription, transgene stability and finally the downstreamingprocessing events for protein purification. Written by aninternational team of industrialists and academics, this bookdescribes: * the fundamental issues associated with expressing heterologousproteins in plants; * a number of detailed examples of the successful small-andlarge-scale production of proteins; * the essentials of patenting; and * the commercial exploitation. Transgenic Plants: A Production System for Industrial andPharmaceutical Proteins will be of interest to plantbiotechnologists, molecular biologists and protein biochemistTable of ContentsPartial table of contents: FUNDAMENTAL ASPECTS IN THE PRODUCTION OF RECOMBINANT PROTEINS INPLANTS. Expression in Plants. The Production of Recombinant Glycoproteins with DefinedNon-Immunogenic Glycans. Downstream Processing of Proteins from Transgenic Plants. Comparison of Host Systems for the Production of RecombinantProteins. Transgene Stability. EXAMPLES. Industrial Enzymes. Pharmaceutical Proteins. COMMERCIALIZATION. Patents and Biotechnology. Commercialization of Phytase-Containing Seed. Index.
£325.76
John Wiley & Sons Inc Handbook of Plant Lectins
Book SynopsisPlant lectins are extensively used as tools and as bioactiveproteins in different areas of biomedical and biological research.The Handbook of Plant Lectins provides a comprehensive yet conciseoverview of the biochemical properties, carbohydrate-bindingspecificity, biological activities and applications of most of thecurrently known plant lectins. This handbook consists of two majorsections: an introductory guide and a quick reference dictionary.Part I acquaints the newcomer to the lectin field with theessential information on lectins and their importance tobiomedicine: * what are lectins? * their carbohydrate-binding specificity * effects on nutrition and immunology * use in histochemistry * application as therapeutic agents Part II lists approximately 200 lectin entries in alphabeticalorder. Each entry deals with the lectin(s) of a particular plantand provides, (where known), details of: * isolation and characterisation; * sugar binding specificity;Table of ContentsINTRODUCTION TO LECTINS. Plant Lectins: A Special Class of Plant Proteins. Plant Lectins in Mammalian Nutrition, Immunology, Metabolism and asOral Therapeutic and Immune Agents. Lectin Histochemistry in Histology and Histopathology. LECTIN CLASSIFICATION. Key to the Use of the Lectin Entries. Classification Codes. Lectin Entries. Appendices. Index.
£334.76
John Wiley & Sons Inc Bioreaction Engineering Bioprocess Monitoring
a huge range and FREE tracked UK delivery on ALL orders.
£341.96
John Wiley & Sons Inc Endocrine and Hormonal Toxicology
Book SynopsisEndocrine and Hormonal Toxicology Edited by Philip W. Harvey, Kevin C. Rush and Andrew Cockburn AgrEvo UK Ltd, Saffron Walden, UK This is the first book to consider the integrated role of the classical endocrine system and hormones (including those from tissues outside the classical endocrine system) in toxicological responses. Although focusing on the latest knowledge on endocrine glands as target organs and the mechanistic and molecular basis for toxicity in these organs, Endocrine and Hormonal Toxicology has been written to cover toxicological responses at the whole body level mediated by endocrine or hormonal mechanisms. This whole body, multi-organ approach significantly broadens the relevance of this volume to toxicologists. Following an introductory section on the types of endocrine toxicity including primary, secondary and indirect mechanisms, the next section deals with endocrine organs as toxicological targets. International contributions focus on the pituitary, thyroid and pTrade Review"section III of this book is a classic" (Clinical Endocrinology, No.53 2000)Table of ContentsSection 1: An Integrated Approach to Endocrine and Hormonal Toxicology Section 2: Endocrine Organs as Toxicological Targets Section 3: Endocrine and Hormonal Toxicology: A Target System Approach Section 4: Endocrine Toxicology: Human and Environmental Health Perspectives
£337.46
John Wiley & Sons Inc Ecotoxicology of Wild Mammals Ecological
Book SynopsisThis landmark book, which contains contributions from the worlda s foremost mammalian ecotoxicologists, is a truly impressive summary of research undertaken in this field in recent years. It is also the first book to draw such information together in a seamless and unified approach.Trade Review"...these 15 papers consider the differing effects of toxins on various species, the accuracy of predictions...and the significance of toxins relative to other threats." (SciTech Book News, Vol. 25, No. 4, December 2001) "excellent reference book" (The BritishToxicology Society Newsletter, December 2001) "I believe this to be a unique reference work in the field of ecotoxicology which should be invaluable to researchers in the field." (Ecotoxicology, Vol.11, No. 75, 2002) "...a superb summary of over 30 years of toxicity studies available on wild mammals...unparalleled in its breadth and depth and should become a desktop reference for anybody in academics or industry who is involved with mammalian ecotoxicology." (Journal of Mammalogoy, Vol. 83, No. 4, November 2002)Table of ContentsList of Contributors. Series Foreword. Editors' Preface. Abbreviations. Environmental Contaminants (D. Hoffman, et al.). Mammal Classification and Characteristics (G. Amori & R. Nowak). Marsupialia and Monotremata (R. Bolton-Grob, et al.). Insectivora (W.-C. Ma & S. Talmage). Chiroptera (D. Clark & R. Shore). Rodentia and Lagomorpha (S. Sheffield, et al.). Carnivora (C. Mason & C. Wren). Pinnipedia (P. Ross & G.Troisi). Cetacea and Sirenia (T. O'Shea & A. Aguilar). Perissodactyla and Artiodactyla (A. Fr?slie, et al.). Biomarkers for Contaminant Exposure and Effects in Mammals (D. Peakall & K. McBee). Extrapolation of Laboratory Tests to Field Populations (D. Forsyth). Assessing Hazard and Risk of Chemical Exposures to Wild Mammals: Food-chain Analysis and its Role in Ecological Risk Assessment (G. Linder & G. Joermann). Putting the Impacts of Environmental Contamination into Perspective (A. Fairbrother). Summation (R. Ringer). Index.
£331.16
Wiley Oxidizing and Reducing Agents
a huge range and FREE tracked UK delivery on ALL orders.
£207.86
Wiley Catalysts for Fine Chemical Synthesis Hydrolysis
Book SynopsisA catalyst is a substance which alters rate at which a chemical reaction occurs but is itself unchanged at the end of the reaction. This book includes approximately 40 proceduces describing the use of different catalysts.Trade Review"Everyone who is concerned with...pure compounds will be able to use this handy book..." (Angewandte Chemie, International Edition, Vol. 42, 2003) "...the book clearly fulfills its purpose to provide a practical introduction?it will be useful..." (CATTECH, August 2003)Table of ContentsSeries Preface. Preface to Volume 1. Abbreviations. PART I: REVIEW. 1. The Integration of Biotransformations into the Catalyst Portfolio. PART II: PROCEDURES. 2. General Information. 3. Asymmetric Epoxidation. 4. Epoxidation of , -Unsaturated Carbonyl Compounds. 5. Epoxidation of Allylic Alcohols. 6. Epoxidation of Unfunctionalized Alkenes and , -Unsaturated Esters. 7. Asymmetric Hydroxylation and Aminohydroxylation. 8. Asymmetric Sulfoxidation. 9. Asymmetric Reduction of Ketones Using Organometallic Catalysts. 10. Asymmetric Reduction of Ketones Using Baker's Yeast. 11. Asymmetric Reduction of Ketones Using Nonmetallic Catalysts. 12. Asymmetric Hydrogenation of Carbon-Carbon Double Bonds Using Organometallic Cataluysts. 13. Employment of Catalysts Working in Tandem. Index.
£233.06
Wiley InsectPlant Interactions and Induced Plant
Book SynopsisInsect-Plant Interactions and Induced Plant DefenceChair: John A. Pickett, 1999 This book examines the sophisticated mechanisms that plants use to defend themselves against attack by insects and pathogens, focusing on the networks of plant signalling pathways that underlie these defences. In response to herbivory, plants release a complex blend of as many as 100 volatile chemicals, known as semiochemicals (''sign chemicals''). These act as an airborne SOS signal, revealing the presence of the herbivore to the predators and parasitoids that are its natural enemies. Plants also have endogenous defence mechanisms that can be induced in response to pathogens, and separate chapters deal with systemic acquired resistance, phytoalexins, and the interacting pathways in pathogen and pest resistance. The book discusses underlying biochemical mechanisms by which plant stress leads to the biosynthesis of chemical signals from pools of secondary metabolite precursors, or even from tTrade Review"...will be of immense value to researchers in the field." --Biologist, November 2000 "...an extremely stimulating book..." --Entomologica Fennica, 13th November 2000Table of ContentsPartial table of contents: Evolutionary Aspects of Plant-Carnivore Interactions (L. Vet). Attraction of Parasitic Wasps by Caterpillar-Damaged Plants (T. Turlings & M. Fritzsche). Specificity of Herbivore-Induced Plant Defences (M. Dicke). Aphids, Predators and Parasitoids (L. Wadhams, et al.). Functional Interactions in the Use of Direct and Indirect Defences in Native Nicotiana Plants (I. Baldwin). Plant Production of Volatile Semiochemicals in Response to Insect-Derived Elicitors (J. Tumlinson, et al.). The Hydroxamic Acid Pathway (A. Gierl & M. Frey). Cross-Talk Between the Signal Pathways for Pathogen-Induced Systemic Acquired Resistance and Grazing-Induced Insect Resistance (G. Felton, et al.). Genetics and Evolution of Insect Resistance in Arabidopsis (T. Mitchell-Olds). Exploiting Insect Responses in Identifying Plant Signals (J. Pickett, et al.). Final Discussion. Indexes.
£144.85
John Wiley and Sons Ltd Introduction to Ecotoxicology
Book SynopsisEnvironmental pollution is one of the most serious threats to the future health of our planet. This book takes a broad view of ecotoxicology starting with the nature, properties and behavior of environmental toxicants, and extends to dose/response relationships and effects on organisms, populations, communities and ecosystems.Table of ContentsPreface. 1 The Ecotoxicology Concept: Introduction. 2 Sources, Types and Properties of Ecotoxicants. 3 Distribution and Transformation of Chemicals in the Environment. 4 Molecular, Biochemical, Physiological and Behavioural Responses.of Organisms. 5 Effects on Population, Community and Ecosystem. 6 Dose and Concentration: Response Relationships. 7 Biomarkers and Biomonitoring of Hazards in the Environment. 8 Ecological Risk Assessment. 9 Ecotoxicology and Management of Chemicals. Index.
£63.86
