Description
Book SynopsisOver the last ten years, much effort has been devoted to improving the biophysical techniques used in the study of viruses. This has resulted in the visualization of these large macromolecular assemblages at atomic level, thus providing the platform for functional interpretation and therapeutic design. Structural Virology covers a wide range of topics and is split into three sections. The first discusses the vast biophysical methodologies used in structural virology, including sample production and purification, confocal microscopy, mass spectrometry, negative-stain and cryo-electron microscopy, X-ray crystallography and nuclear magnetic resonance spectroscopy. The second discusses the role of virus capsid protein structures in determining the functional roles required for receptor recognition, cellular entry, capsid assembly, genome packaging and mechanisms of host immune system evasion. The last section discusses therapeutic strategies based on virus protein structures, including the
Table of ContentsIntroduction: Principles of Virus Structure;Section 1 Methodologies for Structural Analysis of Viruses; Production and Purification of Viruses for Structural Studies; Microscopic (Confocal) Analysis of Viral Entry and Infection; Cryo-electron Microscopy of Virus Infection - Tomography and Asymmetric Structure Determination; Cryo-electron Microscopy of Viruses - 3D of Virus Capsids; X-ray Crystallography of Virus Capsids; Structural Studies of Viral Proteins; Probing Viral Capsid Structures in Solution; Section 2 Structure to Function Correlation for Viruses; Evolution of Viral Capsid Structures - the Three Domains of Life; Mechanisms of Virus Capsid Assembly; Mechanisms of Genome Packaging; Attachment and Viral entry - Receptor Recognition in Viral Pathogenesis; Attachment and Entry - Viral Cell Fusion; Virus Antibody Recognition; Section 3 Therapeutic Strategies Based on Viral Structures; Development of anti-HIV drugs; Design of Influenza Vaccines and Antiviral Agents; Engineering Viral Capsids as Nano Tools; Viral Vectors for Gene Delivery;
