Description
Book SynopsisThe go-to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM)
The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscopegiving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research a
Trade Review
Since the first commercial SEM (scanning electron microscope) was produced by Cambridge Instruments in 1965, the resolution,image contrast, and operability have improved dramatically which have turned field emission SEM into an increasingly valuable tool in the life science community. The challenge of life science microscopy is to provide the structural information for the correlation of structure and function in complex biological systems. For this reason, this two-volume edition comes as a welcome addition to the biologist’s library, since they encompass all the information needed to approach life science FEG SEM imaging challenges, including detailed descriptions of the instrumentation and analytical techniques currently available in the field.
There are many features of this edition that make it a useful resource for both the beginner and more advanced microscopist. First, the two volumes are composed of 31 self-contained chapters, each written by leading authorities on these subjects. Secondly, the chapters are richly illustrated and most chapters are complemented with a comprehensive, valuable, and up-to-date list of references. Accordingly, the user who seeks to purchase new equipment or select the appropriate technique will find useful information in both volumes.
In the initial nine chapters of vol. 1, the reader is given a comprehensive historical review and introduction to the possibilities of the current technology—choice of microscopy method and specimen preparation as well as cryo and room temperature options—edited by representatives from the major companies in the field. The three last chapters of vol. 1 are dedicated to a review of specimen preparation methods—chemical fixation and cryo methods—which are a must-read for any scientist in this field.
In vol. 2 in the first five chapters, the reader is introduced to more special applications and results of the techniques, spanning from pathology and infectious diseases, plant cell wall and nuclear envelope, yeast cell to food research.
The three following chapters focus on cryo-FEGSEM in biology, preparation protocols of vitrified cells for cryo-FIB microscopy, and ESEM (environmental scanning electron microscopy) including many valuable tips and tricks. Opportunities for "Life in 3D" research are described in great detail with chapters on Correlative Array Tomography and the Automatic Tape Collection Microtome followed by chapters on FIB-SEM for Biomaterials, FIB-SEM tomography, and 3D FEGSEM as a tool for structural biology. Vol. 2 is concluded with three chapters on image post-processing and resource management, which are relevant to all scientists—not just FEG SEM microscopists.
In conclusion, I highly recommend Biological Field Emission Scanning Electron Microscopy to anybody who wishes to implement and/or improve an available technology or method. This two-volume edition allows the reader to contemplate how to use existing equipment with some minor adjustments or simple application of sample preparation and analytical tools. Furthermore, this book provides a comprehensive analysis of the latest imaging technologies available in the field and should therefore be invaluable to any researcher who wishes to find a discussion of all these techniques and applications under one umbrella.
—Microscopy and Microanalysis (2020)
Klaus Qvortrup, Faculty of Health Sciences, University of Copenhagen, The Panum Institute, Denmark
Table of ContentsAbout the Editors xix
List of Contributors xxi
Foreword xxv
1 Scanning Electron Microscopy: Theory, History and Development of the Field Emission Scanning Electron Microscope 1
David C. Joy
2 Akashi Seisakusho Ltd – SEM Development 1972–1986 7
Michael F. Hayles
3 Development of FE-SEM Technologies for Life Science Fields 25
Mitsugu Sato, Mami Konomi, Ryuichiro Tamochi and Takeshi Ishikawa
4 A History of JEOL Field Emission Scanning Electron Microscopes with Reference to Biological Applications 53
Kazumichi Ogura and Andrew Yarwood
5 TESCAN Approaches to Biological Field Emission Scanning Electron Microscopy 79
Jaroslav Jiruše, Vratislav Košˇtál and Bohumila Lencová
6 FEG-SEM for Large Volume 3D Structural Analysis in Life Sciences 103
Ben Lich, Faysal Boughorbel, Pavel Potocek and Emine Korkmaz
7 ZEISS Scanning Electron Microscopes for Biological Applications 117
Isabel Angert, Christian Böker, Martin Edelman, Stephan Hiller, Arno Merkle and Dirk Zeitler
8 SEM Cryo-Stages and Preparation Chambers 143
Robert Morrison
9 Cryo–SEM Specimen Preparation Workflows from the Leica Microsystems Design Perspective 167
Guenter P. Resch
10 Chemical Fixation 191
Bruno M. Humbel, Heinz Schwarz, Erin M. Tranfield and Roland A. Fleck
11 A Brief Review of Cryobiology with Reference to Cryo Field Emission Scanning Electron Microscopy 223
Roland A. Fleck, Eyal Shimoni and Bruno M. Humbel
12 High-Resolution Cryo-Scanning Electron Microscopy of Macromolecular Complexes 265
Sebastian Tacke, Falk Lucas, Jeremy D. Woodward, Heinz Gross and Roger Wepf
13 FESEM in the Examination of Mammalian Cells and Tissues 299
Andrew Forge, Anwen Bullen and Ruth Taylor
14 Public Health/Pharmaceutical Research – Pathology and Infectious Disease 311
Paul A. Gunning and Bärbel Hauröder
15 Field Emission Scanning Electron Microscopy in Cell Biology Featuring the Plant Cell Wall and Nuclear Envelope 343
Martin W. Goldberg
16 Low-Voltage Scanning Electron Microscopy in Yeast Cells 363
Masako Osumi
17 Field Emission Scanning Electron Microscopy in Food Research 385
Johan Hazekamp and Marjolein van Ruijven
18 Cryo-FEGSEM in Biology 397
Paul Walther
19 Preparation of Vitrified Cells for TEM by Cryo-FIB Microscopy 415
Yoshiyuki Fukuda, Andrew Leis and Alexander Rigort
20 Environmental Scanning Electron Microscopy 439
Rudolph Reimer, Dennis Eggert and Heinrich Hohenberg
21 Correlative Array Tomography 461
Thomas Templier and Richard H.R. Hahnloser
22 The Automatic Tape Collection UltraMicrotome (ATUM) 485
Anwen Bullen
23 SBEM Techniques 495
Christel Genoud
24 FIB-SEM for Biomaterials 517
Lucille A. Giannuzzi
25 New Opportunities for FIB/SEM EDX in Nanomedicine: Cancerogenesis Research 533
Damjana Drobne, Sara Novak, Andreja Erman and Goran Drai´c
26 FIB-SEM Tomography of Biological Samples: Explore the Life in 3D 545
Caroline Kizilyaprak, Damien De Bellis, Willy Blanchard, Jean Daraspe and Bruno M. Humbel
27 Three-Dimensional Field-Emission Scanning Electron Microscopy as a Tool for Structural Biology 567
J.D. Woodward and R.A. Wepf
28 Element Analysis in the FEGSEM: Application and Limitations for Biological Systems 589
Alice Warley and Jeremy N. Skepper
29 Image and Resource Management in Microscopy in the Digital Age 611
Patrick Schwarb, Anwen Bullen, Dean Flanders, Maria Marosvölgyi, Martyn Winn, Urs Gomez and Roland A. Fleck
30 Part 1: Optimizing the Image Output: Tuning the SEM Parameters for the Best Photographic Results 625
Oliver Meckes and Nicole Ottawa
31 A Synoptic View on Microstructure: Multi-Detector Colour Imaging, nanoflight® 659
Stefan Diller
Index 679
