Description
Book SynopsisG protein-coupled receptors (GPCRs) are the largest single class of receptors in biology, often playing key roles in a remarkably large number of physiological and pathophysiological conditions. GPCRs or GPCR-dependent signalling pathways are the targets of a very large number of therapeutically useful drugs. Detailed knowledge about the molecular structure of GPCRs should therefore pave the way for the design of novel drugs with increased efficacy and specificity.
This volume provides a concise, up-to-date presentation of methods (including molecular genetic, biochemical, and biophysical) which have been used successfully in studying the structure and function of GPCRs. With contributions from international leaders in the field, the editor provides overviews of various techniques, followed by in-depth descriptions of basic procedures and discussions of critical experimental parameters.
Divided into specific, accessible sections, Structure-Function Analysis of G Prote
Table of ContentsPartial table of contents:
Overview of Mutagenesis Techniques (T. Fong).
The Substituted-Cysteine Accessibility Method (J. Javitch).
Metal-Ions as Atomic Scale Probes of G Protein-Coupled Receptor Structure (J. Schetz & D. Sibley).
Genetic Approaches for Studying the Structure and Function of G Protein-Coupled Receptors in Yeast (C. Sommers & M. Dumont).
Electron-Crystallographic Analysis of Two-Dimensional Rhodopsin Crystals (G. Schertler).
Site-Directed Spin-Labeling (SDSL) Studies of the G Protein-Coupled Receptor Rhodopsin (D. Farrens).
Lead Discovery and Development for G Protein-Coupled Receptors (D. Underwood & M. Cascieri).
Index.
