Description
Book SynopsisDespite progress in genetic research, knowledge about the exact structure of the chromosome continues to provide a challenge. Much of that challenge lies with the need for improved tools and methods that researchers require to perform novel analyses beyond the DNA level. Fortunately, rapid advances in nanotechnology, are now being employed to examine, analyze, and manipulate biological material at the chromosome level.
Chromosome Nanoscience and Technology reviews these advances and their contribution to trends and applications in chromosome research. In addition to offering a guide to current progress, this book serves as the culminating report on a Japanese nanobiology project in the field of chromosome science begun in 2000.
The project brought together researchers from disparate backgrounds that included molecular biology, biochemistry, protein science, immunology, genetics, anatomy, semiconductor production, polymer chemistry, material science, microscopy, and infor
Table of Contents
Preface. Mechanical Approaches to Elucidate Mechanisms of Chromosome Condensation at the Nano- and Micro-Level. Development of Novel AFM Probes for Chromosome Manipulation. Microchamber Array-Based Sequence-Specific DNA Detection from a Single Chromosome via Trace Volume PCR. On-Chip Chromosome Sorter Using Electric and Magnetic Fields. Fluorescence Microscopy for Analysis of Chromosome Dynamics. Fluorescence In Situ Hybridization (FISH) as a Tool for Comparative Genomics. Immunocytochemistry for Analyzing Chromosomes. Transmission and Scanning Electron Microscopy of Mammalian Metaphase Chromosomes. Atomic Force Microscopy of Human Chromosomes in Relation to Their Higher-Order Structure. Mechanism of Higher-Order Chromatin Folding Revealed by AFM Observation of In Vitro Reconstituted Chromatin. Scanning Near-Field Optical/Atomic Force Microscopy as a Tool for Simultaneous Specification of Chromosome Topography and Particular Gene Location on the Nanometer Scale. Isolation of Human and Plant Chromosomes as Nano-Materials. Proteome Analysis of Human Metaphase Chromosomes. Anti-Peptide Antibodies for Examining the Conformation and Molecular Assembly of an Intracellular Protein. Structure and Interactions of the Imitation SWI-Type Chromatin-Remodeling Complex, ATP-Dependent Chromatin-Assembly Factor. Dynamic and Functional Analysis of Chromosomal Proteins. Development of a Sustainable Chromosome Imaging Database. Image Database and Image Analysis of Chromosome Information.
