Search results for ""Author Gaspar""

To prepare synchronized cells representing different stages of the cell cycle has been a great challenge for researchers across the globe. In Cell Cycle Synchronization: Methods and Protocols, experts in the field supply detailed protocols providing first the theoretical background of the procedure then step-by-step instructions on how to implement synchronization as well as the latest techniques for the enhanced study of regulatory mechanisms to understand cell cycle events. Describing synchronized cells from asynchronous bacterial, plant, protozoan, yeast, fish, and mammalian cell cultures, the synchronization methods presented in the book are based principally on two major strategies: the “arrest-and-release” approach, which involves different chemical treatments to block cells at certain stages of the cell cycle, and the physical strategy, which contains physical methods to collect cells belonging to subpopulations of the cell cycle. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Detailed and easy to follow, Cell Cycle Synchronization: Methods and Protocols is an ideal guide for scientists who wish to make use of these powerful synchronization techniques but have no access to thorough, time-tested protocols designed for doing so.

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But its waters are also fascinating, interesting and have a wide selection of destinations to enjoy. This book describes 60 safe, viable and feasible routes between the Kent, Essex and Suffolk rivers for safe passage and wholly new time-planning tables which are now simplified with significantly additional and readily available information. The third edition retains all the popular features describing and illustrating each sector of each route, with waypoints and 'rolling road' diagrams, providing information and advice about potential or permanent hazards, the implications for the tide and guidance about shipping routes and anchorages. New simplified passage planning tables It includes new and unique passage planning tables which are simple, accurate and quick to use. Readers can select the most appropriate start time and will instantly see predicted arrival time so as to have sufficient time to continue up river to an ultimate destination. Options are offered for every 30 minutes of the day, whatever the day. The tables not only provide the best predicted start time - for the quickest passage duration - for each route at 4, 5, 6 or 7 knots boat speed, but display alternative passage durations, whatever the tide. Tables provide easier access and a much broader basis of information than in earlier editions, presented alongside more detailed chartlets and a new portrait format book. Sailors benefit from swatchways in the Estuary which shorten time and distance for many passages. However, as they're liable to move or change as sands shift, the author endeavours to survey several critical swatchways, monitors and provides illustrated Notices to Mariners weekly and receives reports from readers. Any significant changes or downloads are reported by updates on the website or by email to readers on the 'Readers' Update' list. www.crossingthethamesestuary.com www.crossingthethamesestuary.com

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