Description
Book SynopsisThe rapid expansion of the TRP field has generated a large amount of excellent original work across many different research fields. However, investigators are not necessarily familiar with the pros and cons of the variety of methods used to study TRP channels. Because of functional and genetic diversity, as well as the different physiological roles they play, techniques used for studying TRP channels range from single molecular analysis to behavioral animal studies. Methods in multiple areas, such as molecular biology, fluorescence imaging, electrophysiology, cell biology, genetics, proteomics, pharmacology, system physiology, and behavioral assessment, are employed to investigate various aspects of these channels. Choosing among many possible topics in these broad areas was a daunting task.
A comprehensive review of the field, TRP Channels spans the information gap by providing broad coverage of current methods and techniques commonly used in TRP channel rese
Table of Contents
High-Throughput Screening of TRPC Channel Ligands Using Cell-Based Assays. Methods to Study TRPC Channel Regulation by Interacting Proteins. Activation of TRP Channels in Mammalian Systems. Studying Subunit Interaction and Complex Assembly of TRP Channels. Proteomic Analysis of TRPC Channels. Lessons of Studying TRP Channels with Antibodies. Assessing TRPC Channel Function Using Pore-Blocking Antibodies. Strategies and Protocols to Generate Mouse Models with Targeted Mutations to Analyze TRP Channel Functions. Studying Endogenous TRP Channels in Visceral and Vascular Smooth Muscles. TRPC Channels in Neuronal Survival. Electrophysiological Methods for the Study of TRP Channels. High-Throughput Approaches to Study Mechanisms of TRP Channel Activation. Methods Used for Studying TRP Channel Functions in Sensory Neurons. Time-Resolved Activation of Thermal TRP Channels by Fast Temperature Jumps Fluorescence Microscopy in Thermo TRP Channel Research. Regulation of TRP Channels by Osmomechanical Stress. Study of TRP Channels in Cancer Cells. In Vitro and In Vivo Assays for the Discovery of Analgesic Drugs Targeting TRP Channels. Studying TRP Channels in Intracellular Membranes. Methods for Studying Drosophila TRP Channels. Studying TRP Channels in Caenorhabditis elegans. Index.
