Description
Book SynopsisThis book provides in-depth information about the ecology, diversity and applications of Actinomycetes. The book is divided into two major parts. The first part discusses the diversity, chemical biology and ecology of Actinomycetes. It also covers the discovery of natural products from soil, endophytic and marine-derived Actinomycetes. It includes natural product discovery, chemical biology, new methods for discovering secondary metabolites, structure elucidation and biosynthetic research of natural products. The chapters in this part focus on the effects of biological and chemical elicitation at molecular level on secondary metabolism in Actinomycetes. The second part of the book discusses genomic and synthetic biology approaches in Actinomycetes drug discovery. This part includes chapters focused on the application of metabolic engineering to optimize natural product synthesis and the use of omics data in engineering of regulatory genes. It covers the advanced tools of synthetic biology and metabolic engineering including cluster assembly, CRISPR/Cas9 technologies, and chassis strain development for natural product overproduction in Actinomycetes. It describes the use of bioinformatics tools for reprogramming of biosynthetic pathways through polyketide synthase and non-ribosomal peptide synthetase engineering. These advanced genomic and molecular tools are expected to accelerate the discovery and development of new natural products from Actinomycetes with medicinal and other industrial applications. The book is useful to researchers and students in the field of microbiology, pharmaceutical sciences and drug discovery.
Table of ContentsSection 1: Diversity, Chemical biology and Ecology of Actinomycetes; and the Discovery of Natural Products
Chapter 1: An overview on Natural products from Actinomycetes: their discovery and biosynthesis.
Chapter 2: Isolation, identification (cultural and molecular techniques), and systematics of Actinomycetes from various sources (marine, soil, extremophilic environment).
Chapter 3: Studies on diversity of nonribosomal peptide synthetase and polyketide synthase gene clusters among taxonomically close Actinomycetes strains.
Chapter 4: Chemical elicitors and signaling molecules and their role in elicitation of secondary metabolism in Actinomycetes.
Chapter 5: Regulation of antibiotic biosynthesis genes in Actinomycetes at molecular level.
Chapter 6: Screening strategies for inhibitors against cancer cells from natural Products repository of Actinomycetes.
Chapter 7: Rare and endophytic Actinomycetes in the biosynthesis of bioactive metabolites.
Chapter 8: Advances in bioactivity guided isolation of natural products from Actinomycetes.
Chapter 9: Natural product discovery from non-Streptomyces Actinomycetes.
Chapter 10: Use of high-efficiency CRISPR/Cas9-mediated genome editing to study biology of Actinomycetes.
Section 2: Genomic approaches and synthetic biology tools in Actinomycetes drug discovery and industrial compounds
Chapter 11: An overview of biomedical, biotechnological and industrial applications of Actinomycetes
Chapter 12: Genomics and proteomics approach in natural product discovery in Actinomycetes.
Chapter 13: Structural dereplication and strain dereplication strategies and metabolomics in natural product research.
Chapter 14: Genome mining of rare Actinomycetes and cryptic pathways for secondary metabolites.
Chapter 15: Glycosylated bioactive compound discovery by from Actinomycetes by combination of genome mining, gene inactivation and activation of silent biosynthetic clusters.
Chapter 16: Genetic engineering for overproducing bioactive secondary metabolites in Actinomycetes
Chapter 17: Transcriptional gene cluster refactoring for increased secondary metabolites production
Chapter 18: Synthetic biology in Actinomycetes for natural product discovery
Chapter 19: Metabolic engineering of antibiotic factories: new tools for antibiotic production in Actinomycetes
Chapter 20: Bioinformatic tools for use in pks and nrps discovery in Actinomycetes.
