Description
Book SynopsisMost research in the life sciences involves a core set of molecular-based equipment and methods, for which there is no shortage of step-by-step protocols. Nonetheless, there remains an exceedingly high number of inquiries placed to commercial technical support groups, especially regarding problems.
Trade Review"It will strengthen your ability to prevent, identify and solve problems in the lab..." (Medical Laboratory World, October 2001)
Table of ContentsPreface.
Contributors.
Preparing for Success in the Laboratory (P. Franciskovich).
Getting What You Need from a Supplier (T. Tyre & G. Krueger).
The Preparation of Buffers and Other Solutions: A Chemist's Perspective (E. Pfannkoch).
How to Properly Use and Maintain Laboratory Equipment (T. Troutman, et al.).
Working Safely with Biological Samples (C. Haidaris & E. Brownlow).
Working Safely with Radioactive Materials (W. Volny).
DNA Purification (S. Herzer).
RNA Purification (L. Martin, et al.).
Restriction Endonucleases (D. Robinson, et al.).
Nucleotides, Oligonucleotides, and Polynucleotides (A. Gerstein).
PCR (K. Aoyagi).
Electrophoresis (M. Booz).
Western Blotting (P. Riis).
Nucleic Acid Hybridization (S. Herzer & D. Englert).
E. coli Expression Systems (P. Bell).
Eukaryotic Expression (J. Trill, et al.).
Index.
