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Book Synopsis

Plant virus nanoparticle (VNP) is a useful platform with biocompatibility and versatile monodisperse protein structures that can be engineered with bioactive cues, offering opportunities to functionalize bioinert hydrogels for tissue engineering. The hypothesis in this study was that osteogenesis of human mesenchymal stem cells (hMSCs) and biomineralization could be enhanced by incorporating VNPs, which were engineered with osteogenesis-associated or cell-adhesive peptides, into cell-laden hydrogels. Cellular responses to VNPs were examined in both 2D and 3D cultures, including VNP-cell distribution, cell attachment, morphology, and osteogenesis. VNP-laden agarose or agarose-collagen hydrogels were characterized in terms of release rate, mineralization effect, mechanical properties, and usage as bioink. The results revealed enhanced osteogenic differentiation when cells were cultured on VNP-coated surfaces, and attachment of VNPs to cells as well as at least 84 % of VNP retention we

Material and cell biological characterization of

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    A Paperback by Ph.D. Lin Dr Ying-Ying

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      Publisher: Verlag G. Mainz
      Publication Date: Publication Date: 01/09/2021
      ISBN13: 9783958864276, 978-3958864276
      ISBN10: 3958864279

      Description

      Book Synopsis

      Plant virus nanoparticle (VNP) is a useful platform with biocompatibility and versatile monodisperse protein structures that can be engineered with bioactive cues, offering opportunities to functionalize bioinert hydrogels for tissue engineering. The hypothesis in this study was that osteogenesis of human mesenchymal stem cells (hMSCs) and biomineralization could be enhanced by incorporating VNPs, which were engineered with osteogenesis-associated or cell-adhesive peptides, into cell-laden hydrogels. Cellular responses to VNPs were examined in both 2D and 3D cultures, including VNP-cell distribution, cell attachment, morphology, and osteogenesis. VNP-laden agarose or agarose-collagen hydrogels were characterized in terms of release rate, mineralization effect, mechanical properties, and usage as bioink. The results revealed enhanced osteogenic differentiation when cells were cultured on VNP-coated surfaces, and attachment of VNPs to cells as well as at least 84 % of VNP retention we

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