Description
Book SynopsisComprehensive coverage of the entire induced pluripotent stem cell basic work flow
Pluripotent stem cells (PSC) can divide indefinitely, self-renew, and can differentiate to functionally reconstitute almost any cell in the normal developmental pathway, given the right conditions. This comprehensive book, which was developed from a training course, covers all of the PSCs (embryonic, embryonic germ, and embryonic carcinoma) and their functions. It demonstrates the feeder-dependent and feeder-free culture of hESC and hiPSC, which will be referred to in all protocols as PSCs. It also addresses the methods commonly used to determine pluripotency, as defined by self-renewal marker expression and differentiation potential.
Human Pluripotent Stem Cells: A Practical Guide offers in-depth chapter coverage of introduction to stem cell, PSC culture, reprogramming, differentiation, PSC characterization, and more. It also includes four appendixes containing information
Table of Contents
1 Introduction 1
1.1 Biosafety 1
1.2 Biosafety Levels 2
1.3 Aseptic Technique 2
1.4 Storage 6
1.5 Contamination 7
1.6 Pluripotent Stem Cells 8
1.7 Procedures 9
References 16
2 Pluripotent Stem Cell Culture 17
2.1 Introduction 17
2.2 Materials 18
2.3 Solutions 19
2.4 Methods 21
References 47
3 Reprogramming 49
3.1 Introduction 49
3.2 Materials 49
3.3 Solutions 54
3.4 Methods 57
References 97
4 Characterization 99
4.1 Introduction 99
4.2 Materials 101
4.3 Solutions 103
4.4 Methods 104
References 128
5 Differentiation 131
5.1 Introduction 131
5.2 Materials 131
5.3 Solutions 133
5.4 Methods 135
References 151
Index 153
