Description
Book SynopsisGene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
Table of Contents1. An Overview of Gene Targeting and Stem Cells 2. Advances in Gene Targeting in Murine Embryonic Stem Cells 3. Nuclear Transfer with Murine ES Cells 4. Gene Targeting in Sheep 5. RNA Interference 6. Human Embryonal Carcinoma Cells: Surrogate Tools in the Study of Human Embryonic Stem Cells 7. Isolation of Human ES Cells 8. In Vitro Culture and Differentiation of Human Embryonic Stem Cells 9. Transfection of Human Embryonic Stem Cells
