Description
Book SynopsisThis textbook introduces readers to the modern light microscope. It can either be used alone or in conjunction with a practical course. The book begins with an account of how the light microscope works, and how to set up the instrument for optimum performance.
Table of ContentsAbout the Author ix
Acknowledgements xi
Look-Up Guide to Feature Boxes by Theme xii
Glossary and Definitions xiv
Notes xxiv
Introduction xxvii
1 Our Eyes and the Microscope 1
2 Light 29
3 Basic Microscope Optics 55
4 Microscope Anatomy and Design 75
5 Ergonomics 91
6 Optical Aberrations of the Microscope 101
7 The Microscope Objective 127
8 Condensers and Eyepieces 161
9 Illumination in the Microscope 177
10 Diffraction and Image Formation in Microscopy 211
11 Contrast Generation and Enhancement 243
12 Reflected-Light Microscopy 289
13 Polarised-Light Microscopy: Part 1 – Theory 317
14 Polarised-Light Microscopy: Part 2 – Applied 347
15 Fluorescence Microscopy 383
16 Fluorophores and Fluorescent Proteins 405
17 Optical Sectioning and Confocal Microscopy 425
18 Operating the Confocal Microscope 447
19 Light-Sheet Microscopy 483
20 Bleed-Through and Spectral Unmixing 507
21 Deconvolution 523
22 Multi-Photon Microscopy 543
23 Total Internal Reflection Fluorescence Microscopy 561
24 FRAP and FRET 569
25 Colocalisation 587
26 Super-Resolution Fluorescence Microscopy 613
27 Choosing a Microscope Platform and Core Imaging Facilities 637
28 Biological Specimen Preparation 663
29 Materials Specimen Preparation 687
30 Recording the Image: Part 1 – Theory 707
31 Recording the Image: Part 2 – Applied 733
Appendices
1 Buying, and Tendering for, a Light Microscope 769
2 Troubleshooting Poor Image Quality 773
3 The Michel-Lévy Interference Colour Chart 775
4 Cleaning and Maintenance of the Light Microscope 779
5 Selected Suppliers 783
6 Historical Background 787
7 Timeline of Key Events 791
Index 799
