Description
Book SynopsisPlant-parasitic and free-living nematodes are increasingly important in relation to food security, quarantine measures, ecology (including pollution studies), and research on host-parasite interactions. Being mostly microscopic, nematodes are challenging organisms for research. Techniques for Work with Plant and Soil Nematodes introduces the basic techniques for laboratory and field work with plant-parasitic and free-living soil-dwelling nematodes. Written by an international team of experts, this book is extensively illustrated, and addresses both fundamental traditional techniques and new methodologies. The book covers areas that have become more widespread over recent years, such as techniques used in diagnostic laboratories, including computerized methods to count and identify nematodes. Information on physiological assays, electron microscopy techniques and basic information on current molecular methodologies and their various applications is also included. This book is an essential resource for students of nematology and parasitology, academic researchers, diagnostic laboratories, and quarantine and advisory service personnel. It provides a much-needed methodology standard for anyone involved in work on plant and soil nematodes.
Table of Contents1: Sampling 2: Methods for Nematode Extraction 3: Estimating Numbers 4: Screening Plants for Resistance/Susceptibility to Plant-parasitic Nematodes 5: Handling, fixing, staining and mounting nematodes 6: Culturing Techniques 7: Preparation of Figures, Measuring and Image Processing 8: Electron microscopy techniques 9: Behavioural and Physiological Assays 10: Staining Chromosomes 11: Isoelectric Focusing of Proteins 12: Molecular Identification of Nematodes using Polymerase Chain Reaction (PCR) 13: Isolation and Characterisation of Tandem Repeats in Nematode Genomes 14: Characterization of Nematode Mitochondrial Genomes 15: Phylogenetic Analysis of DNA Sequence Data
