Description
Book SynopsisSecond-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical applications. The book features contributions by experts in second-harmonic imaging, including many pioneering researchers in the field. Written for researchers at all levels, it takes an in-depth look at the current state of the art and possibilities of SHG microscopy.
Organized into three sections, the book:
- Provides an introduction to the physics of the process, step-by-step instructions on how to build an SHG microscope, and comparisons with related imaging techniques
- Gives an overview of the capabilities of SHG microscopy for imaging tissues and cellsincl
Trade Review
"... comprehensive material of fundamentals, mechanisms, theory, practical implementation, and multiple practical applications of the novel and prospective nonlinear optical imaging method. The editors are to be congratulated for this outstanding work, for collecting excellent chapters, and for book editing. ... useful information for experts in the field and also can serve as a tutorial for master's and PhD students specializing in biomedical engineering and medical science." -Microscopy and Microanalysis, August 2014 "This book provides up-to-date coverage of our current understanding of harmonic imaging principles and also the most important biomedical applications of this technique." -From the Foreword by Peter T.C. So, Harvard University
Table of Contents
Principles of SHG Imaging: Theoretical Framework for SHG Microscopy: A Matrix-Based Approach. How to Build an SHG Apparatus. THG Microscopy of Cells and Tissues: Contrast Mechanisms and Applications. SHG Microscopy and Its Comparison with THG, CARS, and Multiphoton Excited Fluorescence Imaging. Biomedical Imaging Using SHG: Molecular Structure and Order with Second-Harmonic Generation Microscopy. 3D SHG Imaging and Analysis of Fibrillar Collagen Organization. Second-Harmonic Generation Imaging of Microtubules. SHG and Optical Clearing. Holographic SHG Imaging. Imaging Membrane Potential with SHG. Applications of SHG: Second-Harmonic Generation Imaging of Self-Assembled Collagen Gels. SHG and Multiphoton Fluorescence Imaging of the Eye. Multiphoton Excitation Imaging of the Arterial Vascular Bed. Combined SHG/THG Imaging. SHG Imaging of Collagen and Application to Fibrosis Quantization. Quantitative Approaches for Studying the Role of Collagen in Breast Cancer Invasion and Progression. SHG in Tumors: Scattering and Polarization. SHG Imaging for Tissue Engineering Applications. Index.
