Description

Book Synopsis
A comprehensive introduction to the art and science used by the biochemist and process engineer in the design and optimization of large-scale protein-purification processes. Covers the basics of proteins'' properties, the unit operations involved in protein purification as well as important related issues of process design, integration with upstream operations, cost and timing to market. Includes an abundance of figures and tables.

Table of Contents
Process Design and Competitive Advantage.

Strategies for Process Design.

Protein Characteristics.

Analytical Methods.

Impact of Upstream Operations.

Cell Disruption.

Centrifugation.

Precipitation and Extraction.

Filtration.

General Chromatography.

Ion Exchange Chromatography.

Hydrophobic Interaction Chromatography and Reversed PhaseChromatography.

Affinity Chromatography.

Gel Filtration Chromatography.

Large-Scale Examples.

Index.

Protein Purification

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    A Hardback by Scott M. Wheelwright

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      Book details

      Publisher John Wiley & Sons Inc
      Published 23 May 1994
      ISBN-13 9780471037231
      978-0471037231
      ISBN-10 0471037230
      Also in:
      Chemistry Industrial applications of scientific research Polymer chemistry

      Description

      Book Synopsis
      A comprehensive introduction to the art and science used by the biochemist and process engineer in the design and optimization of large-scale protein-purification processes. Covers the basics of proteins'' properties, the unit operations involved in protein purification as well as important related issues of process design, integration with upstream operations, cost and timing to market. Includes an abundance of figures and tables.

      Table of Contents
      Process Design and Competitive Advantage.

      Strategies for Process Design.

      Protein Characteristics.

      Analytical Methods.

      Impact of Upstream Operations.

      Cell Disruption.

      Centrifugation.

      Precipitation and Extraction.

      Filtration.

      General Chromatography.

      Ion Exchange Chromatography.

      Hydrophobic Interaction Chromatography and Reversed PhaseChromatography.

      Affinity Chromatography.

      Gel Filtration Chromatography.

      Large-Scale Examples.

      Index.

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