Description
Book SynopsisThis second edition has been extended to include new information and developments in the preservation of fungi but maintains the coverage of methodology as presented in the first edition published in 1983. The book enables readers to select suitable preservation procedures for their purposes, gives information on the growth and maintenance of fungi and provides the background to manage a collection of microorganisms both safely and effectively. The methods described are those in operation or experimented upon at IMI, and cover the simple to the more complex techniques. The methods listed are of use in all situations where living cultures of fungi are required, from teaching and research to industry and technology.
Table of Contents1: Foreword 2: Microbial resource collections: aims, management and information 3: Collection management 4: Acquisition of strains 5: Accession 6: Preservation and storage 7: Culture properties 8: Distribution 9: Information 10: Continuous growth and adaptations for storage 11: Growth 12: Methods of culture maintenance 13: Sub-culturing 14: Storage under mineral oil 15: Water storage 16: Summary of maintenance by growth techniques 17: Drying and freeze-drying 18: Drying 19: Silica gel storage 20: Soil storage 21: Freeze-drying 22: Factors affecting survival in freeze-drying 23: Cryopreservation 24: Method 25: Optimization of preservation conditions 26: Selection of preservation techniques 27: Recommended methods of preservation for taxonomic groups 28: Selection of preservation technique based upon collection requirements 29: Comparison of methods available 30: Suitability of the techniques for fungi 31: Summary 32: Special techniques and procedures 33: Cleaning and mite prevention 34: Discarding cultures 35: Cryogenic light microscopy 36: Means of achieving reproducible cooling rates
