Optogenetics represents a breakthrough technology capable of dynamically modulating molecular and cellular activity in live cells with high precision. This transformative technology made it possible to reversibly interrogate protein actions, cellular events, and animal behaviors with a simple flash of light. Manipulating the temporal and spatial profile of light enabled precise control of membrane potentials. This feature has inspired non-opsin-based optogenetics, which not only inherits the precise spatiotemporal resolution but also expands the targets into a diverse pool of biomolecules such as membrane receptors, ion channels, kinases, GTPases, and transcription factors. This book is unique because it emphasizes the design and applications of opsin-free optogenetic tools.
- Describes new developments in non-opsin-based optogenetic tools
- Introduces cutting-edge methods for precise modulation of cell signaling
Trade Review
“This book differs markedly from these other, more general volumes by virtue of its concentration on next generation, non-opsin photo-switches. The result is a focused, highly informative, yet compact and easy-to-read volume that offers a model for how these works should be constructed.”
Doody Review Peter J. Kennelly, PhD (Virginia Tech)
Table of Contents
Table of Content
Chapter 1
Optogenetic dissection of a two-component calcium influx pathway
Xiaoxuan Liu, Yingshan Wang, Yubin Zhou, and Guolin Ma*
Chapter 2
High-throughput engineering of a light-activatable Ca2+ channel
Lian He*, Liuqing Wang, and Youjun Wang*
Chapter 3
Optogenetic activation of TrkB signaling
Peiyuan Huang, Zhihao Zhao, Lei Lei, and Liting Duan*
Chapter 4
Spatiotemporal modulation of neural repair
Huaxun Fan, Qin Wang, Kai Zhang*, and Yuanquan Song*
Chapter 5
Optogenetic control of neural stem cell differentiation
Yixun Su, Taida Huang, Kai Zhang, and Chenju Yi*
Chapter 6
An optogenetic toolbox for remote control of programmed cell death
Ningxia Zhang and Ji Jing*
Chapter 7
Control of protein levels in Saccharomyces cerevisiae by optogenetic modules that act on protein synthesis and stability
Sophia Hasenjäger, Jonathan Trauth, and Christof Taxis*
Chapter 8
Optogenetics as a tool to study neurodegeneration and signal transduction
Prabhat Tiwari* and Nicholas S. Tolwinski*
Chapter 9
Opsin-free optogenetics: brain and beyond
Jongryul Hong, Yeonji Jeong, and Won Do Heo*
Chapter 10
Constructing a far-red light-induced split-Cre recombinase system for controllable genome engineering
Meiyan Wang, Jiali Wu, and Haifeng Ye*
Chapter 11
Tools and Technologies for Wireless and Non-invasive Optogenetics
Guangfu Wu, Vagif Abdulla, Yiyuan Yang, Michael Schneider, and Yi Zhang*
