Description
Book SynopsisNeisseria meningitidis is a major causative agent, worldwide, of potentially life-threatening meningitis and septicaemia, which carry with them a high mortality rate and permanent physical and neurological issues for survivors. Neisseria meningitidis: Advanced Methods and Protocols offers a collection of methods and protocols that reflect the development and refinement of several new technologies applied to the meningococcus as presented by expert researchers. Several of the chapters in this book describe methods that rely on the collection of complete sets of biological data, for example, using the genome to generate transcriptomes, proteomes, and metabolomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Table of Contents
1. Neisseria meningitidis: Biology, Microbiology, and Epidemiology
Nadine G. Rouphael and David S. Stephens
2. Classification and Pathogenesis of Meningococcal Infections
Petter Brandtzaeg and Marcel van Deuren
3. Detection of Neisseria meningitidis in Cerebrospinal Fluid Using a Multiplex PCR and the Luminex Detection Technology
Jens Kjølseth Møller
4. Generating Knock-Out and Complementation Strains of Neisseria meningitides
Vincent van Dam and Martine P. Bos
5. Identification and Functional Characterization of sRNAs in Neisseria meningitides
Yvonne Pannekoek and Arie van der Ende
6. Expression, Purification, and Crystallization of Neisserial Outer Membrane Proteins
Muhammad Saleem, Jeremy Moore, and Jeremy P. Derrick
7. Genome-Scale Metabolic Models: Reconstruction and Analysis
Gino J.E. Baart and Dirk E. Martens
8. TMT Labeling for the Quantitative Analysis of Adaptive Responses in the Meningococcal Proteome
Karsten Kuhn, Christian Baumann, Jan Tommassen, and Thorsten Prinz
9. Meningococcal Ligands and Molecular Targets of the Host
Darryl J. Hill and Mumtaz Virji
10. In Vivo Imaging of Meningococcal Disease Dynamics
Hong Sjölinder and Ann-Beth Jonsson
11. Methods for Studying Neisseria meningitidis Biofilms
Michael A. Apicella, Jianqiang Shao, and R. Brock Neil
12. A Laminar-Flow Chamber Assay for Measuring Bacterial Adhesion Under Shear Stress
Magali Soyer and Guillaume Duménil
13. Techniques to Measure Pilus Retraction Forces
Nicolas Biais, Dustin Higashi, Magdalene So, and Benoit Ladoux
14. Human Dendritic Cell Culture and Bacterial Infection
Hannah E. Jones, Nigel Klein, and Garth L.J. Dixon
15. Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry (H/DX-MS) to Investigate Ligand-Receptor Interactions
Jessmi M.L. Ling, Leslie Silva, David C. Schriemer, and Anthony B. Schryvers
16. Visualizing PAMP-PRR Interactions Using Nanoscale Imaging
Kathy Triantafilou and Martha Triantafilou
17. Transcriptome Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells
Biju Joseph, Matthias Frosch, Christoph Schoen, and Alexandra Schubert-Unkmeir
18. The Use of the Pan-Neisseria Microarray and Experimental Design for Transcriptomics Studies of Neisseria
Nigel J. Saunders and John K. Davies
19. Analysis of Parameters Associated with Prevention of Cellular Apoptosis by Pathogenic Neisseriae and Purified Porins
Paola Massari and Lee M. Wetzler
20. Analysis of the Immune Response to Neisseria meningitidis Using a Proteomics Approach
Jeannette N. Williams, Myron Christodoulides, and John E. Heckels
21. Antigen Identification Starting from the Genome: A "Reverse Vaccinology" Approach Applied to MenB
Emmanuelle Palumbo, Luigi Fiaschi, Brunella Brunelli, Sara Marchi, Silvana Savino, and Mariagrazia Pizza
22. A DNA Vaccine Strategy for Effective Antibody Induction to Pathogen-Derived Antigens
Jason Rice and Myron Christodoulides
