Description

Book Synopsis

Minimal Perturbation Analysis of mRNA Degradation Rates with Tet Off and RT qPCR.- Northern Blotting: Protocols for Radioactive and Non-Radioactive Detection of RNA.- RNA Blotting by Electrotransfer and RNA Detection.- Dynamic Evolution of Poly-A Tail Lengths Visualized by RNAse H Assay and Northern Blot Using Non-Radioactive Probes in Yeast.- Assessing Deadenylation Activity by Polyacrylamide Gel Electrophoresis Using a Fluorescent RNA Substrate.- Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay.- Purification of Enzymatically Active Xrn1 for Removal of Non-Capped mRNAs from In Vitro Transcription Reactions and Evaluation of mRNA Decapping Status In Vivo.- Production of Fully Capped mRNA for Transfection into Mammalian Cells: A Protocol for Enzymatic Degradation of Uncapped Transcripts after In Vitro mRNA Synthesis.- Transcriptome-Wide Analysis of the 5' Cap Status of RNA Using 5Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing.- RNA Decay Assay:

mRNA Decay

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    A Hardback by Maxime Wery

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      Publisher: Humana
      Publication Date: 11/14/2024
      ISBN13: 9781071641750, 978-1071641750
      ISBN10: 1071641751
      Also in:
      Molecular biology

      Description

      Book Synopsis

      Minimal Perturbation Analysis of mRNA Degradation Rates with Tet Off and RT qPCR.- Northern Blotting: Protocols for Radioactive and Non-Radioactive Detection of RNA.- RNA Blotting by Electrotransfer and RNA Detection.- Dynamic Evolution of Poly-A Tail Lengths Visualized by RNAse H Assay and Northern Blot Using Non-Radioactive Probes in Yeast.- Assessing Deadenylation Activity by Polyacrylamide Gel Electrophoresis Using a Fluorescent RNA Substrate.- Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay.- Purification of Enzymatically Active Xrn1 for Removal of Non-Capped mRNAs from In Vitro Transcription Reactions and Evaluation of mRNA Decapping Status In Vivo.- Production of Fully Capped mRNA for Transfection into Mammalian Cells: A Protocol for Enzymatic Degradation of Uncapped Transcripts after In Vitro mRNA Synthesis.- Transcriptome-Wide Analysis of the 5' Cap Status of RNA Using 5Monophosphate-Dependent Exonuclease Digestion and RNA Sequencing.- RNA Decay Assay:

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