Description
Book SynopsisThis second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume III guides readers through chapters on intercellular mitochondrial transfer, Cytoplasmic transfer methods, Ndufs4 knockout mouse, in vivo assessment of mitochondrial oxygen consumption, mitochondrial network analysis by imaging, mitochondrial respiration in platelets, Heterologous Inferential Analysis (HIA), bioenergetic profiling, integrative methods for studying cardiac energetics, and isolation and deep sequencing of mitochondrial DNA.Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible,Mitochondrial Medicine, Second Edition, Volume 3: Manipulating Mitochondria and Disease- Speci
Table of Contents1. Allotopic Expression of ATP6 in the Mouse as a Transgenic Model of Mitochondrial Disease
David A. Dunn and Carl A. Pinkert
2. Mitochondrial Transplantation for Ischemia Reperfusion Injury
Ilias P. Doulamis and James D. McCully
3. Quantitatively Controlled Intercellular Mitochondrial Transfer by Cell Fusion-Based Method Using a Microfluidic Device
Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, and Mizuo Maeda
4. Lipophilic Conjugates for Carrier-Free Delivery of RNA Importable Into Human Mitochondria
Ilya Dovydenko, Mariya Meschaninova, Anne-Marie Heckel, Ivan Tarassov, Alya Venyaminova, and Nina Entelis
5. Drug Discovery Assay to Identify Modulators of the Mitochondrial Ca2+ Uniporter
Daniela M. Arduino, Valerie Goh, Dejana Mokranjac, and Fabiana Perocchi
6. Cytoplasmic Transfer Methods for Studying the Segregation of Mitochondrial DNA in Mice
Thomas Kolbe, Ralf Steinborn, and Joerg P. Burgstaller
7. Electron Attachment to Isolated Molecules as a Probe To Understand Mitochondrial Reductive Processes
Stanislav A. Pshenichnyuk
and Alberto Modelli
8. Assessment of Short- and Medium-Chain Fatty Acids on Mitochondrial Function in Severe Inflammation
Matthias Hecker, Natascha Sommer, and Konstantin Mayer
9. Assessment of the Effects of Drugs on Mitochondrial Respiration
Jana Hroudová and Zdeněk Fišar
10. The Ndufs4 Knockout Mouse: A Dual Threat Model of Childhood Mitochondrial Disease and Normative Aging
Anthony S. Grillo, Alessandro Bitto, and Matt Kaeberlein
11. Suborganellar Localisation of Mitochondrial Proteins and Transcripts in Human Cells
Anna Smirnova, Ludovic Richert, Alexandre Smirnov, Yves Mély, and Ivan Tarassov
12. In vivo Assessment of Mitochondrial Oxygen Consumption
Floor A. Harms and Egbert G. Mik
13. Update of Mitochondrial Network Analysis by Imaging: Proof of Technique in Schizophrenia
Yekaterina Yatchenko and Dorit Ben-Shachar
14. Heterologous Inferential Analysis (HIA) and other Emerging Concepts
In Understanding Mitochondrial Variation in Pathogenesis: There is no More Low Hanging Fruit
Antón Vila-Sanjurjo, Paul M. Smith, and Joanna L. Elson
15. Accurate Measurement of Cellular and Cell-Free Circulating Mitochondrial DNA Content from Human Blood Samples Using Real Time Quantitative PCR
Hannah Rosa and Afshan Malik
16. Measurement of Mitochondrial Respiration in Platelets
Zdeněk Fišar and Jana Hroudová
17. Isolation and Electron Microscopic Analysis of Liver Cancer Cell Mitochondria
Yaschar Kabiri, Carola Eberhagen, Sabine Schmitt, Percy A. Knolle, and Hans Zischka
18. Assessment of Mitochondrial Reactive Oxygen Species and Redox Regulation in Stem Cells
Madhavee Thumiah-Mootoo, Tina Podinic, and Mireille Khacho
19. Single Cell Approaches for Studying the Role of Mitochondrial DNA in Neurodegenerative Disease
Laura J. Bailey, Joanna L. Elson, and Ilse S. Pienaar
20. A New Method for Sequencing the Mitochondrial Genome by Using Long Read Technology
Sophie Dhorne-Pollet, Nicolas Pollet, and Eric Barrey
21. Mitochondrial DNA as a Sensitive Biomarker of UV-induced Cellular Damage in Human Skin
Amy Bowman and Mark A. Birch-Machin
22. Isolation of Mitochondria-associated ER Membranes (MAMs), Synaptic MAMs and Glycosphingolipid Enriched Microdomains (GEMs) from Brain Tissues and Neuronal Cells
Ida Annunziata, Jason Andrew Weesner, and Alessandra d’Azzo
23. Mitochondrial Diagnostics: A Discovery-Based Biochemical Platform for Phenotyping Human Peripheral Blood Cell Mitochondria
Margaret A. M. Nelson and Kelsey H. Fisher-Wellman
24. Bioenergetic Profiling Of Human Pluripotent Stem Cells
Gizem Inak-Girrbach, Marie-Thérèse Henke, and Alessandro Prigione
25. Integrative Methods for Studying Cardiac Energetics
Philippe Diolez, Véronique Deschodt-Arsac, Guillaume Calmettes, Gilles Gouspillou, Laurent Arsac, Pierre Jais, Michel Haissaguerre, and Pierre dos Santos
26. Isolation of Mitochondria from Retinal Pigment Epithelial Cell Cultures and an Application Of High Resolution Respirometric Assay (XF
e96 Seahorse assay)
Masaaki Ishii, Gyda Beeson, Craig Beeson, and Bärbel Rohrer
27. The Isolation and Deep Sequencing of Mitochondrial DNA
Alexander G. Bury, Fiona M. Robertson, Angela Pyle, Brendan A. I. Payne, and Gavin Hudson
28. Ultrastructure of the Mitochondria-Associated Membranes in Human Tumor Specimens
Gabriel Arismendi-Morillo
