Description
Book SynopsisChapters consists of six sections detailing modification of microtubule structures, observation and control of microtubule movement, material applications of microtubules in vitro, development of microtubule-binding molecules, comprehensive approaches to analyze properties of microtubules, and functionalization of microtubules in living cells.
Table of ContentsPART I: Preparation and Modification of Microtubules
1. Purification of Tubulin from Porcine Brain and its Fluorescence Dye Modification
Satsuki Ishii, Mousumi Akter, Jakia Jannat Keya, Mst. Rubaya Rashid, Farhana Afroze, Syeda Rubaiya Nasrin, and Akira Kakugo
2. Facile Method of Tubulin Purification from Goat Brain for Reconstitution of Microtubule Associated Intracellular Function
Satyajit Ghosh, Shubham Garg, Nabanita Mukherjee, and Surajit Ghosh
3. Functionalization of Tubulin: Approaches to Modify Tubulin with Biotin and DNA
Mousumi Akter, Jakia Jannat Keya, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, Satsuki Ishii, and Akira Kakugo
4. Electro-Modulation of Tubulin Properties and Function
Djamel Eddine Chafai and Michal Cifra
PART II: Observation and Control of Microtubule Movement
5. In Vitro Reconstitution of Microtubule Dynamics and Severing Imaged by Label-Free Interference Reflection Microscopy
Yin-wei Kuo and Jonathon Howard
6. Characterizing the Number of Kinesin Motors Bound to Microtubules in the Gliding Motility Assay using FLIC Microscopy
Virginia VanDelinder and George D. Bachand
7. Design of Mechanical and Electrical Properties for Multi-Directional Control of Microtubules
Hang Zhou, Taikopaul Kaneko, Naoto Isozaki, and Ryuji Yokokawa
8. Linear-Zero Mode Waveguides for Single-Molecule Fluorescence Observation of Nucleotides in Kinesin-Microtubule Motility Assay
Kazuya Fujimoto, Ryota Iino, and Ryuji Yokokawa
9. Microtubules and Quantum Dots Integration Leads to Conjugates with Applications in Biosensors and Bionanodevices
Xiao Hu and Cerasela Zoica Dinu
PART III: Microtubule-Based Active Matters
10. Assembling Microtubule-Based Active Matter
Alexandra M. Tayar, Linnea M. Lemma, and Zvonimir Dogic
11. Spontaneous Alignment of Microtubules via Tubulin Polymerization in a Narrow Space under a Temperature Gradient
Kazuhiro Shikinaka
12. Dynamic Pattern Formation of Active Matters Triggered by Mechanical Stimuli
Jakia Jannat Keya, Arif Md. Rashedul Kabir, Mousumi Akter, and Akira Kakugo
13. Spontaneously Beating Biomimetic Structures
Isabella Guido
14. Construction of Molecular Robots from Microtubules for Programmable Swarming
Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Mst. Rubaya Rashid, and Akira Kakugo
15. Fabrication of Artificial Muscle from Microtubules, Kinesins and DNA Origami Nanostructures
Jakia Jannat Keya, Mousumi Akter, Arif Md. Rashedul Kabir, Satsuki Ishi, and Akira Kakugo
PART IV: Microtubule-Binding Molecules
16. Encapsulation of Nanomaterials Inside Microtubules by Using a Tau-derived Peptide
Hiroshi Inaba and Kazunori Matsuura
17. Investigating Tubulin-Drug Interaction Using Fluorescence Spectroscopy
Anuradha Kumari and Dulal Panda
PART V: Analysis of Microtubule Dynamics and Structures
18. Visualization and Quantification of Microtubule Self-repair
Jérémie Gaillard, Laurent Blanchoin, Manuel Théry, and Laura Schaedel
19. Cargo Transport by Microtubule-Associated Motor Protein Along Mechanically Deformed Microtubules
Syeda Rubaiya Nasrin, Arif Md. Rashedul Kabir, and Akira Kakugo
20. Mechanical Deformation of Microtubules on a Two-Dimensional Elastic Medium
Syeda Rubaiya Nasrin, Farhana Afroze, Arif Md. Rashedul Kabir, and Akira Kakugo
21. Real-Time Imaging of Single γTuRC-Mediated Microtubule Nucleation Events In Vitro by TIRF Microscopy
Tanja Consolati, Gil Henkin, Johanna Roostalu, and Thomas Surrey
22. Microtubule Preparation for Investigation with High-Speed Atomic Force Microscopy
Christian Ganser and Takayuki Uchihashi
23. Crystallization Systems for the High-Resolution Structural Analysis of Tubulin-Ligand Complexes
Tobias Mühlethaler, Natacha Olieric, Valentin A. Ehrhard, Maximilian Wranik, Jörg Standfuss, Ashwani Sharma, Andrea E. Prota, and Michel O. Steinmetz
24. Cryo-EM Visualization of Neuronal Particles Inside Microtubules
Sylvie Gory-Fauré, Julie Delaroche, Camille Cuveillier, Christian Delphin, and Isabelle Arnal
25. Reconstituting the Interaction Between Purified Nuclei and Microtubule Network
Gokce Agsu, Jérémie Gaillard, Bruno Cadot, Laurent Blanchoin, Emmanuelle Fabre, and Manuel Théry
PART VI: Modulation of Cellular Microtubules
26. Photocontrolling Microtubule Dynamics with Photoswitchable Chemical Reagents
Oliver Thorn-Seshold and Joyce C.M. Meiring
27. Monitoring the Disruptive Effects of Tubulin Binding Agents on Cellular Microtubules
Anuradha Kumari and Dulal Panda
28. Pacific Blue-Taxoids as Fluorescent Molecular Probes of Microtubules
Angelo E. Andres, Digamber Rane, and Blake R. Peterson
29. Controlling Cell Shape and Microtubule Organization by Extracellular Matrix Micropatterning
Alessandro Dema, Shima Rahgozar, Laurent Siquier, Jeffrey van Haren, and Torsten Wittmann
