Description
Book SynopsisThis volume contains several reviews in the field as well as the step-by-step use of targeted and global approaches within the areas of genomics, epigenetics, proteomics, transcriptomics and metabolomics which aim to address this dilemma and to help pinpoint new treatment strategies. Chapters detail the generation of several models and methods for assessing health and provide researchers potential approaches for reversing or minimizing effects of disease. In addition, important information on disease mechanisms is provided, as each method is described in the context of a specific disease or therapeutic area. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical,Investigations of Early Nutrition Effects on Long-Ter
Table of ContentsPart I: Reviews
1. Nutritional Programming Effects on Development of Metabolic Disorders in Later Life
Thomas Prates Ong and Paul C. Guest
2. Effects of Prenatal Nutrition and the Role of the Placenta in Health and Disease
Leslie Myatt and Kent L Thornburg
3. Developmental Origins of Stress and Psychiatric Disorders
Francesca L Guest and Paul C. Guest
4. Proteomic Studies of Psychiatric Disorders
Paul C. Guest
5. Developmental Origins of Breast Cancer: A Paternal Perspective
Camile Castilho Fontelles, Raquel Santana da Cruz, Leena Hilakivi-Clarke, Sonia de Assis, and Thomas Prates Ong
6. Point-of-care Testing and Personalized Medicine for Metabolic Disorders
Francesca L. Guest and Paul C. Guest
7. Pregnancy and Lactation a Window of Opportunity to Improve Individual Health
Guadalupe L. Rodríguez-González, Diana C Castro-Rodríguez, and Elena Zambrano
8. Utility of Small Animal Models of Developmental Programming
Clare M Reynolds and Mark H Vickers
Part II: Protocols
9. Generation of Maternal Obesity Models in Studies of Developmental Programming in Rodents
Paul D Taylor, Phillippa A Matthews, Imran Y Khan, Douglas Rees, NozomiItani, Lucilla Poston
10. Generation of the Maternal Low Protein Rat Model for Studies of Metabolic Disorders
Dan Ma, Susan E. Ozanne, and Paul C. Guest
11. Investigation of Paternal Programming of Breast Cancer Risk in Female Offspring in Rodent Models
Camile Castilho Fontelles, Raquel Santana da Cruz, Leena Hilakivi-Clarke, Sonia de Assis, and Thomas Prates Ong
12. Studies of Isolated Peripheral Blood Cells as a Model of Immune Dysfunction
Hassan Rahmoune and Paul C. Guest
13. Studies of a Neuronal Cell Line as a Model of Psychiatric Disorders
Keiko Iwata
14. Assessment of Placental Transport Function in Studies of Disease Programming
Amanda N. Sferruzzi-Perri
15. Assessment of Fatty Liver in Models of Disease Programming
Kimberley D Bruce and Karen R Jonscher
16. Capillary Blood Sampling from the Finger
Kieron Rooney
17. Physical Activity Assessment in Clinical Studies of Substance use Disorder
Rhiannon Dowla, Bridin Murnion, Kia Roberts, Jonathan Freeston, and Kieron Rooney
18. Cardiopulmonary Exercise Testing
Derek Tran
19. Cardiovascular Assessment in Human Research
Marjan Mosalman Haghighi and Julian Ayer
20. Nutritional Programming Effects on the Immune System
Donald B. Palmer
21. Small RNA Sequencing - A Technique for miRNA Profiling
Lucas Carminatti Pantaleão and Susan E. Ozanne
22. Pulse-chase Biosynthetic Radiolabelling of Pancreatic Islets to Measure Beta Cell Function
Paul C. Guest
23. Rapid and Easy Protocol for Quantification of Next Generation Sequencing Libraries
Steve F.C. Hawkins and Paul C. Guest
24. Telomere length analysis - A Tool for Dissecting Ageing Mechanisms in Developmental Programming
Jane L. Tarry-Adkins and Susan E. Ozanne
25. Qualitative and quantitative NMR Approaches in Blood Serum Lipidomics
Banny Silva Barbosa, Lucas Gelain Martins, TássiaB. B. C. Costa, Guilherme Cruz, and LjubicaTasic
26. In vivo Electrical Stimulation for the Assessment of Skeletal Muscle Contractile Function in Murine Models
Kaio F. Vitzel, Marco A. Fortes, Gabriel N. Marzuca-Nassr, Maria V.M. Scervino, Carlos H. Pinheiro, Leonardo R. Silveira, and Rui Curi
27. Experimental Model of Skeletal Muscle Laceration in Rats
Phablo Abreu, Gabriel Nasri Marzuca-Nassr, Sandro Massao Hirabara, and Rui Curi
28. Neuropsychiatric Sequelae of Early Nutritional Modifications: A Beginner’s Guide to Behavioural Analysis
Ann-Katrin Kraeuter, Paul C. Guest, and Zoltán Sarnyai
29. Hyperlocomotion Test for Assessing Behavioural Disorders
Dan Ma and Paul C. Guest
30. 2D-DIGE Analysis of Eye Lens Proteins as a Measure of Cataract Formation
Paul C. Guest
31. Mass Spectrometry Profiling of Pituitary Glands
Divya Krishnamurthy, Hassan Rahmoune, and Paul C. Guest
32. Multiplex Immunoassay Profiling of Hormones Involved in Metabolic Regulation
Laurie Stephen and Paul C. Guest
33. Time Resolved Fluorescence Assays for Quantification of Insulin Precursors in Plasma and Serum
Kevin Taylor, Ian Halsall, Paul C. Guest, and Keith Burling
34. Identification of Neural Stem Cell Biomarkers by Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) Mass Spectrometry
Paul C. Guest
35. Lab-on-a-chip Device for Rapid Measurement of Vitamin D Levels
Harald Peter, Nikitas Bistolas, Soeren Schumacher, Cecilia Laurisch, Paul C. Guest, Ulrich Höller, and Frank F. Bier
36. Kidney Smartphone Diagnostics
PR Matías-García and JL Martinez-Hurtado
37. A User-friendly App for Blood Coagulation Disorders
Johannes Vegt and Paul C. Guest
38. Hormonal Smartphone Diagnostics
PR Matías-García, JL Martinez-Hurtado, A Beckley, M Schmidmayr, and V Seifert-Klauss
