Description
Book SynopsisA comprehensive introduction to the blood-brain barrier and methodology of its study which will be an important information resource for a wide range of medical specialists from researcher to clinician. Advances in both methodology and biology of the blood-brain barrier are detailed in 50 chapters from international authorities.
Trade Review"This book, introducing the essential methodologies, biology and pathology of the blood-brain barrier, is welcome, especially when edited by a known expert in the field, William M. Pardridge...recommended reading to those wishing to enter this challenging area of drug delivery" Journal of Controlled Release
Table of ContentsList of contributors; 1. Blood-brain barrier methodology and biology; Part I. Methodology: 2. The carotid artery single injection technique; 3. Development of the Brain Efflux Index (BEI) method and its application to the blood-brain barrier efflux transport study; 4. In situ brain perfusion; 5. Intravenous injection/pharmacokinetics; 6. Isolated brain capillaries: an in vitro model of blood-brain barrier research; 7. Isolation and behavior of plasma membrane vesicles made from cerebral capillary endothelial cells; 8. Patch clamp technique with isolated brain microvessel membranes; 9. Tissue culture of brain endothelial cells - induction of blood-brain barrier properties by brain factors; 10. Brain microvessel endothelial cell culture systems; 11. Intracerebral microdialysis; 12. Blood-brain barrier permeability measured with histochemistry; 13. Measuring local cerebral capillary permeability-surface area products by quantitative autoradiography; 14. Measurement of blood-brain barrier permeability in humans using indicator diffusion; 15. Measurement of blood-brain barrier permeability in humans with positron emission tomography; 16. Magnetic resonance imaging of blood-brain barrier permeability; 17. Molecular biology of brain capillaries; Part II. Transport Biology: 18. Biology of the blood-brain glucose transporter; 19. Glucose transporters in mammalian brain development; 20. Blood-brain barrier amino acid transport; 21. P-glycoprotein, a guardian of the brain; 22. Blood-brain barrier ion transport; 23. Ion channels in endothelial cells; 24. Interactions of lipoproteins with the blood-brain barrier; 25. Fatty acid and lipid intermediate transport; 26. Blood-brain barrier transport of drugs; Part III. General Aspects of CNS Transport: 27. The blood-CSF barrier and the choroid plexus; 28. Arachnoid membrane, subarachnoid CSF and pia-glia; 29. Circumventricular organs of the brain; 30. Transport in the developing brain; Part IV. Signal Transduction/Biochemical Aspects: 31. Regulation of brain endothelial cell tight junction permeability; 32. Chemotherapy and chemosensitization; 33. Lipid composition of brain microvessels; 34. Brain microvessel antigens; 35. Molecular dissection of tight junctions: occludin and ZO-1; 36. Phosphatidylinositol pathways; 37. Nitric oxide and endothelin at the blood-brain barrier; 38. Role of intracellular calcium in regulation of brain endothelial permeability; 39. Cytokines and the blood-brain barrier; 40. Blood-brain barrier and monoamines, revisited; Part V. Pathophysiology in Disease States: 41. Cerebral amyloid angiopathy; 42. Brain microvasculature in multiple sclerosis; 43. Hemostasis and the blood-brain barrier; 44. Microvascular pathology in cerebrovascular ischemia; 45. HIV infection and the blood-brain barrier; 46. Hypertension; 47. The blood-brain barrier in brain tumours; 48. The pathophysiology of blood-brain barrier dysfunction due to traumatic brain injury; 49. Cerebral malaria and the brain microvasculature; 50. Molecular basis of tissue tropism of bacterial meningitis.
